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[Review Of] Fake Geek Girls: Fandom, Gender, And The Convergence Culture Industry, By Suzanne Scott, Kyra Hunting

Journalism and Media Faculty Publications

No abstract provided.

Develop A High-Throughput Screening Method To Identify C-P4h1 (Collagen Prolyl 4-Hydroxylase 1) Inhibitors From Fda-Approved Chemicals, Shike Wang, Kuo-Hao Lee, Nathália Victoria Araujo, Chang-Guo Zhan, Vivek M. Rangnekar, Ren Xu

Pharmaceutical Sciences Faculty Publications

Collagen prolyl 4-hydroxylase 1 (C-P4H1) is an α-ketoglutarate (α-KG)-dependent dioxygenase that catalyzes 4-hydroxylation of proline on collagen. C-P4H1-induced prolyl hydroxylation is required for proper collagen deposition and cancer metastasis. Therefore, targeting C-P4H1 is considered a potential therapeutic strategy for collagen-related cancer progression and metastasis. However, no C-P4H1 inhibitors are available for clinical testing, and the high content assay is currently not available for C-P4H1 inhibitor screening. In the present study, we developed a high-throughput screening assay by quantifying succinate, a byproduct of C-P4H-catalyzed hydroxylation. C-P4H1 is the major isoform of collagen prolyl 4-hydroxylases (CP4Hs) that contributes the majority prolyl 4-hydroxylase …

Upregulation Of Cpt1a Is Essential For The Tumor-Promoting Effect Of Adipocytes In Colon Cancer, Xiaopeng Xiong, Yang-An Wen, Rachelle Fairchild, Yekaterina Y. Zaytseva, Heidi L. Weiss, B. Mark Evers, Tianyan Gao

Markey Cancer Center Faculty Publications

Colon tumors grow in an adipose tissue-enriched microenvironment. Locally advanced colon cancers often invade into surrounding adipose tissue with a direct contact with adipocytes. We have previously shown that adipocytes promote tumor growth by modulating cellular metabolism. Here we demonstrate that carnitine palmitoyltransferase I (CPT1A), a key enzyme controlling fatty acid oxidation (FAO), was upregulated in colon cancer cells upon exposure to adipocytes or fatty acids. In addition, CPT1A expression was increased in invasive tumor cells within the adipose tissue compared to tumors without direct contact with adipocytes. Silencing CPT1A abolished the protective effect provided by fatty acids against nutrient …

Misinformation More Likely To Use Non-Specific Authority References: Twitter Analysis Of Two Covid-19 Myths, Joseph Mcglynn, Maxim Baryshevtsev, Zane A. Dayton

Communication Graduate Research

This research examines the content, timing, and spread of COVID-19 misinformation and subsequent debunking efforts for two COVID-19 myths. COVID-19 misinformation tweets included more non-specific authority references (e.g., “Taiwanese experts”, “a doctor friend”), while debunking tweets included more specific and verifiable authority references (e.g., the CDC, the World Health Organization, Snopes). Findings illustrate a delayed debunking response to COVID-19 misinformation, as it took seven days for debunking tweets to match the quantity of misinformation tweets. The use of non-specific authority references in tweets was associated with decreased tweet engagement, suggesting the importance of citing specific sources when refuting health misinformation.

Trait-Based Root Phenotyping As A Necessary Tool For Crop Selection And Improvement, Rebecca K. Mcgrail, David A. Van Sanford, David H. Mcnear Jr.

Plant and Soil Sciences Faculty Publications

Most of the effort of crop breeding has focused on the expression of aboveground traits with the goals of increasing yield and disease resistance, decreasing height in grains, and improvement of nutritional qualities. The role of roots in supporting these goals has been largely ignored. With the increasing need to produce more food, feed, fiber, and fuel on less land and with fewer inputs, the next advance in plant breeding must include greater consideration of roots. Root traits are an untapped source of phenotypic variation that will prove essential for breeders working to increase yields and the provisioning of ecosystem …

Everything Is Science: A Free City-Wide Science Festival, Jarrod W. Creameans, Michelle G. Pitts, Olivia White, Kellen M. Greenwell, Kristie Colón, Sylvie Garneau-Tsodikova, Vincent J. Venditto

Pharmaceutical Sciences Faculty Publications

A week-long, city-wide science festival called Everything is Science (EiS) was developed to educate the community in an informal manner. The festival serves as a platform for presenters from diverse professions to give engaging talks (without PowerPoint slides) to the public, free of charge, in restaurants and bars around town. Over 350 people attended the events over 5 days with 33 presenters. Surveys completed by attendees and session coordinators indicate strong support for this festival. Altogether, the EiS festival serves as a no-cost method to engage with the community and improve science literacy with potential for adoption in other cities.

Lstm Forecasts For Smart Home Electricity Usage, Rosemary E. Alden, Huangjie Gong, Cristinel Ababei, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

With increasing of distributed energy resources deployment behind-the-meter and of the power system levels, more attention is being placed on electric load and generation forecasting or prediction for individual residences. While prediction with machine learning based approaches of aggregated power load, at the substation or community levels, has been relatively successful, the problem of prediction of power of individual houses remains a largely open problem. This problem is harder due to the increased variability and uncertainty in user consumption behavior, which make individual residence power traces be more erratic and less predictable. In this paper, we present an investigation of …

Rapid In Vitro Multiplication Of Non-Runnering Fragaria Vesca Genotypes From Seedling Shoot Axillary Bud Explants, Babul C. Sarker, Douglas D. Archbold, Robert L. Geneve, Sharon T. Kester

Horticulture Faculty Publications

Fragaria vesca L. has become a model species for genomic studies relevant to important crop plant species in the Rosaceae family, but generating large numbers of plants from non-runner-producing genotypes is slow. To develop a protocol for the rapid generation of plants, leaf explants were compared to single axillary bud shoot explants, both from in vitro-grown Fragaria vesca seedlings, as sources of shoots for new plant production in response to benzyladenine (BA) or thidiazuron (TDZ) combined with indolebutyric acid (IBA) on Murashige and Skoog’s Basal Salt (MS) medium. BA at 2.0 and 4.0 mg L⁻¹ and TDZ at 1.5 …

Discussing The Gender Gap In The Venue Management Industry: It’S An Interesting Idea But..., Jill Schinberg

Arts Administration Faculty Publications

Gender disparity is nothing new—not in the arts and culture sector nor the various administrative fields of arts and entertainment management. However, our willingness to bring gender representation issues and equal opportunity to light has changed over time. In 2017, the International Association of Venue Managers took the first steps toward a more inclusive industry. This paper examines the impetus for the first diversity survey of its kind in the public assembly sphere, discusses the data as they pertain to gender, assesses the association’s first conference session on the topic, contextualizes the results, and proposes directions for future research in …

Some Basics Of Capitalism And Socialism And Implications For Human Liberty, Morality, And Fairness, John Garen

Institute for the Study of Free Enterprise Working Papers

This essay outlines the big-picture aspects of capitalism and socialism, and uses this overview to discuss human liberty and economic liberty in each system. Additionally, I note that some have argued that capitalism is unfair or immoral and so I consider three specific and common moral standards in this regard. They are: (i) “From each according to his ability, to each according to his needs;” (ii) “People should get what they earn;” and (iii) “’Agreement without conformity’ or ‘conformity without agreement.’” Capitalism is centered on individual rights and private ownership, while socialism awards decision-making powers to government. The former is …

Designing Works In Sangallo’S ‘Circle’, Julia Smyth-Pinney

Architecture Faculty Publications

No abstract provided.

Staying Inspired In Difficult Times, Jennifer A. Bartlett

Library Faculty and Staff Publications

This issue's column suggests a few new releases to prompt professional reflection and renewal.

The Effect Of High Efficiency Building Technologies And Pv Generation On The Energy Profiles For Typical Us Residences, Evan S. Jones, Rosemary E. Alden, Huangjie Gong, Andrew G. Frye, Donald G. Colliver, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

The penetrations of high efficiency technologies and photovoltaic (PV) generation are increasing in the residential sector. Technologies such as improved insulation and efficient HVAC systems significantly affect the energy profile of a house. This effect varies due to climate characteristics, i.e. temperature, solar radiation, relative humidity, and wind speeds. The effect of other technologies, such as efficient water heaters, lighting, or kitchen appliances, is mainly governed by human behavior, which may be represented by a schedule. This paper studies the performance of both climate-influenced and scheduled household devices among different levels of efficiency through combined computational and experimental methods. Three …

On The Feasibility Of Electrification For Large Mobile Cranes, Donovin Lewis, Damien Lawhorn, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Trends towards vehicle electrification to reduce dependence on fossil fuels and increase drive train efficiency have led vehicle manufacturers to seek out paths towards gradual hybridization. For heavy duty construction vehicles, electrification consists of two principle components: electric hybridization of the vehicle carrier and the vehicle's auxiliary function. Economic and physical feasibility for the transition to electrical replacements for critical system components is important for the gradual development of electrified systems. In this paper, we present an investigation into multiple pathways for the hybridization of mobile cranes paired with simulations that analyze the feasibility of system electrification. ADVISOR was used …

Teaching Undergraduates With Primary Sources 2020 Research Study Report, Jay-Marie Bravent, Deirdre Scaggs, Matthew Strandmark, Danielle Gabbard

Library Reports and White Papers

This report presents the findings of an exploratory examination of the pedagogical practices of social sciences and humanities instructors who teach undergraduates with primary sources at the University of Kentucky (UK). Conducted in December 2019 and January 2020 by a research team from the University of Kentucky Libraries Special Collections Research Center, the study reveals areas of success within existing programs and services, the benefits and drawbacks of teaching with digitized primary sources, as well as inherent pedagogical challenges to overcome. A list of recommendations based on the findings seeks to address these challenges and concludes the report. As part …

Longer Service Life Bridge Coatings, Danny Wells, Theodore Hopwood, Bobby Meade, Sudhir Palle

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) has maintenance oversight responsibilities for approximately 1,100 steel bridges; it also helps local governments and other entities with maintenance painting on other steel bridges. Because maintaining steel bridges is costly, Cabinet officials asked Kentucky Transportation Center (KTC) researchers to identify materials and methods that will prolong their service lives. Steel bridges are uniquely vulnerable to deterioration resulting from exposure to atmospheric conditions (e.g. moisture, ultraviolet rays) and chlorides, the latter due to the application of deicing materials. This study examines two strategies for extending service lives. First, it appraises a novel method of hot dip …

Vulnerabilities To Online Social Network Identity Deception Detection Research And Recommendations For Mitigation, Max Ismailov, Michail Tsikerdekis, Sherali Zeadally

Information Science Faculty Publications

Identity deception in online social networks is a pervasive problem. Ongoing research is developing methods for identity deception detection. However, the real-world efficacy of these methods is currently unknown because they have been evaluated largely through laboratory experiments. We present a review of representative state-of-the-art results on identity deception detection. Based on this analysis, we identify common methodological weaknesses for these approaches, and we propose recommendations that can increase their effectiveness for when they are applied in real-world environments.

Potential For Bacteriophage Cocktail To Complement Commercial Sanitizer Use On Produce Against Escherichia Coli O157:H7, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan

Animal and Food Sciences Faculty Publications

The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p < 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p < 0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential …

Network Architecture For Generating A Labeled Overhead Image, Nathan Jacobs, Scott Workman

Computer Science Faculty Patents

A computer-implemented process is disclosed for generating a labeled overhead image of a geographical area. A plurality of ground level images of the geographical area is retrieved. A ground level feature map is generated, via a ground level convolutional neural network, based on features extracted from the plurality of ground level images. An overhead image of the geographical area is also retrieved. A joint feature map is generated, via an overhead convolutional neural network based on the ground level feature map and features extracted from the plurality of ground level images. Geospatial function values at a plurality of pixels of …

Effect Of Niacin Monotherapy On High Density Lipoprotein Composition And Function, Scott M. Gordon, Marcelo J. Amar, Kianoush Jeiran, Michael Stagliano, Emma Staller, Martin P. Playford, Nehal N. Mehta, Tomas Vaisar, Alan T. Remaley

Saha Cardiovascular Research Center Faculty Publications

BACKGROUND: Niacin has modest but overall favorable effects on plasma lipids by increasing high density lipoprotein cholesterol (HDL-C) and lowering triglycerides. Clinical trials, however, evaluating niacin therapy for prevention of cardiovascular outcomes have returned mixed results. Recent evidence suggests that the HDL proteome may be a better indicator of HDL's cardioprotective function than HDL-C. The objective of this study was to evaluate the effect of niacin monotherapy on HDL protein composition and function.

METHODS: A 20-week investigational study was performed with 11 participants receiving extended-release niacin (target dose = 2 g/day) for 16-weeks followed by a 4-week washout period. HDL …

Scale And Numerical Modeling To Determine Operating Points Of A Non-Clogging Vortecone Filter In Mining Operation, Ashish R. Kumar, Sampurna Arya, Adam Levy, Steven Schafrik, William C. Wedding, Kozo Saito

Progress in Scale Modeling, an International Journal

Numerical and scale modeling studies of Vortecone, a pressure-driven wet-scrubber device for the efficient capture of over-sprayed paint in the automobile industry, are presented. In this manuscript, Vortecone was tested for removing dust particles from underground mining operations. The pressures required to operate Vortecone and the airflow rates through the Vortecone are the two most important factors for mine ventilation systems. This study used dry, no-particle conditions to obtain relationship between these two parameters and then designed a Vortecone filter ventilation system for mining operations. Commercial software, SC/Flow, was used to generate the CFD model with unstructured meshes and a …

The Behavior Of Micro Explosive Charge Underwater Explosion Near A Rigid Wall, Kazutaka Kitagawa, Atsushi Abe

Progress in Scale Modeling, an International Journal

The interactions between an underwater explosion and underwater structures is a research topic related to understanding strong impulsive forces for disaster preparation and prevention. This study is a part of series studying the behavior of underwater explosion bubbles near different boundaries and structures because understanding the boundary phases and the pulsation of bubbles could be a useful predictive tool. Micro explosive underwater explosions were conducted by detonating a very small amount of silver azide; the time evolution and attenuation of effects from the explosion were studied. Both numerical and experimental data were acquired and compared for underwater shock waves, gas …

Scaling Realistic Fire Scenarios, James G. Quintiere

Progress in Scale Modeling, an International Journal

A review is made of work on scale modeling in fire and presented from the experience of the author. Primarily, scale modeling in air is discussed but there is a brief discussion of a scale model with salt and fresh water for smoke movement. A complete set of dimensionless groups is presented for fire, then it is illustrated how selections were made for the partial scaling of specific fire scenarios. Studies have been motivated by basic research interests as well as for fire investigations. The dynamics of floorcovering fire spread in a corridor is studied to reveal many features of …

Therapeutic Trem2 Activation Ameliorates Amyloid-Beta Deposition And Improves Cognition In The 5xfad Model Of Amyloid Deposition, Brittani R. Price, Tiffany L. Sudduth, Erica M. Weekman, Sherika Johnson, Danielle Hawthorne, Abigail E. Woolums, Donna M. Wilcock

Sanders-Brown Center on Aging Faculty Publications

BACKGROUND: Triggering receptor expressed on myeloid cell-2 (TREM2) is a lipid and lipoprotein binding receptor expressed by cells of myeloid origin. Homozygous TREM2 mutations cause early onset progressive presenile dementia while heterozygous, point mutations triple the risk of Alzheimer's disease (AD). Although human genetic findings support the notion that loss of TREM2 function exacerbates neurodegeneration, it is not clear whether activation of TREM2 in a disease state would result in therapeutic benefits. To determine the viability of TREM2 activation as a therapeutic strategy, we sought to characterize an agonistic Trem2 antibody (AL002a) and test its efficacy and mechanism of action …

Method And System For Terpene Production Platforms In Yeast, Joseph Chappell, Xun Zhuang, Shuiqin Wu

Pharmaceutical Sciences Faculty Patents

A method is provided for producing modified mutant yeast and the resulting yeast that can be used as a platform for terpene production. The method includes chemical mutagenesis to effect ergosterol dependent growth in yeast. Subsequently, these yeast are subjected to an erg9 knockout mutation to thereby produce ergosterol dependent growth/ erg9 knockout mutation yeast cell lines. The resulting yeast are well suited for use in the production of terpenes.

Near Simultaneous Laser Scanning Confocal And Atomic Force Microscopy (Conpokal) On Live Cells, Joree N. Sandin, Surya P. Aryal, Thomas E. Wilkop, Christopher I. Richards, Martha E. Grady

Physiology Faculty Publications

Techniques available for micro- and nano-scale mechanical characterization have exploded in the last few decades. From further development of the scanning and transmission electron microscope, to the invention of atomic force microscopy, and advances in fluorescent imaging, there have been substantial gains in technologies that enable the study of small materials. Conpokal is a portmanteau that combines confocal microscopy with atomic force microscopy (AFM), where a probe "pokes" the surface. Although each technique is extremely effective for the qualitative and/or quantitative image collection on their own, Conpokal provides the capability to test with blended fluorescence imaging and mechanical characterization. Designed …

The Effect Of Bacterial Endotoxin Lps On Serotonergic Modulation Of Glutamatergic Synaptic Transmission, Jate Bernard, Abigail Greenhalgh, Oscar Istas, Nicole T. Marguerite, Robin L. Cooper

Biology Faculty Publications

The release of the endotoxin lipopolysaccharides (LPS) from gram-negative bacteria is key in the induction of the downstream cytokine release from cells targeting cells throughout the body. However, LPS itself has direct effects on cellular activity and can alter synaptic transmission. Animals experiencing septicemia are generally in a critical state and are often treated with various pharmacological agents. Since antidepressants related to the serotonergic system have been shown to have a positive outcome for septicemic conditions impacting the central nervous system, the actions of serotonin (5-HT) on neurons also exposed to LPS were investigated. At the model glutamatergic synapse of …

Are College Students’ Attitudes Related To Their Application Of Sanctions For Campus Sexual Assault Cases?, Jaspreet K. Chahal, Caihong R. Li, Diane R. Follingstad, Claire M. Renzetti

CRVAW Faculty Journal Articles

With growing attention to adjudication of campus sexual assault cases, more is known regarding students’ views of sexual assault, but little the literature focuses on how students perceive “justice” in terms of assigning sanctions or guilt/responsibility for such cases. The present study focused on understanding whether college students’ preformed attitudes and beliefs were associated with the severity of sanctions they applied across a range of sexual assault cases as well as their assignments of guilt and responsibility to the parties involved. To determine students’ attitudes and beliefs mediating effects on sanction choices, five scales (i.e., rape myth acceptance, downplaying the …

An Aggregated And Equivalent Home Model For Power System Studies With Examples Of Building Insulation And Hvac Control Improvements, Huangjie Gong, Evan S. Jones, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Introducing new technologies into existing residences provides opportunities for the utility to enhance a community's power system performance. To validate the benefits of such technologies, an energy model is required in which their integration with a community's power system may be simulated. It can be difficult or impossible to properly model a power system without sufficient sample data. This paper proposes a method that uses Gaussian kernel density estimation (GKDE) to calculate the aggregated net power flow of a community's distribution system with only limited sample points at each time step. Example case studies that confirm the usefulness of the …

Telemedicine In The Emergency Department In The Era Of Covid-19: Front-Line Experiences From 2 Institutions, Joel M. Hamm, Chris Greene, Michael T. Sweeney, Setareh Mohammadie, Linda B. Thompson, Eric Wallace, Walter Schrading

Emergency Medicine Faculty Publications

During the COVID-19 pandemic, one of the major changes that has occurred in emergency medicine is the evolution of telemedicine. With relaxation of regulatory and administrative barriers, the use of this already available technology has rapidly expanded. Telemedicine provides opportunity to markedly decrease personal protective equipment (PPE) and reduce healthcare worker exposures. Moreover, with the convenience and availability of access to medical care via telemedicine, a more fundamental change in healthcare delivery in the United States is likely. The implementation of telemedicine in the emergency department (ED) in particular has great potential to prevent the iatrogenic spread of COVID-19 and …