Digital Commons Network^™

Fusion And Beyond: Satellite Cell Contributions To Loading-Induced Skeletal Muscle Adaptation, Kevin A. Murach, Christopher S. Fry, Esther E. Dupont-Versteegden, John J. Mccarthy, Charlotte A. Peterson

Center for Muscle Biology Faculty Publications

Satellite cells support adult skeletal muscle fiber adaptations to loading in numerous ways. The fusion of satellite cells, driven by cell-autonomous and/or extrinsic factors, contributes new myonuclei to muscle fibers, associates with load-induced hypertrophy, and may support focal membrane damage repair and long-term myonuclear transcriptional output. Recent studies have also revealed that satellite cells communicate within their niche to mediate muscle remodeling in response to resistance exercise, regulating the activity of numerous cell types through various mechanisms such as secretory signaling and cell–cell contact. Muscular adaptation to resistance and endurance activity can be initiated and sustained for a period of …

Competitive Rationales: Beneath The Surface Of Competitive Behavior, Goce Andrevski, Danny Miller, Isabelle Le Breton-Miller, Walter Ferrier

Management Faculty Publications

Competitive dynamics research has focused on studying whether rivals are able and likely to carry out competitive actions, typically by examining indirect reasons such as characteristics of the actions themselves, the firms involved, or the competitive context. We explore why rivals initiate a specific competitive action at a particular time and situation. Drawing from the philosophy of action literature, we introduce the concept of competitive rationales to examine the primary reasons that cause tactical actions. Given the rapid exchanges characterizing tactical competitive dynamics, we conducted an inductive, multicase study to explore the reasons behind over 800 discrete tactical decisions carried …

Knowledge And Beliefs Associated With Environmental Health Literacy: A Case Study Focused On Toxic Metals Contamination Of Well Water, Kathleen M. Gray, Victoria Triana, Marti Lindsey, Benjamin Richmond, Anna G. Hoover, Chris Wiesen

Preventive Medicine and Environmental Health Faculty Publications

Environmental health literacy (EHL) is developing as a framework that can inform educational interventions designed to facilitate individual and collective action to protect health, yet EHL measurement poses several challenges. While some studies have measured environmental health knowledge resulting from interventions, few have incorporated skills and self-efficacy. In this study, a process-focused EHL instrument was developed, using the Newest Vital Sign (NVS) health literacy instrument as a model and tailoring it for the context of private well contamination with toxic metals. Forty-seven (47) participants, including undergraduate students and residents of communities with contaminated well water, piloted a prototype EHL instrument …

In Situ Imaging Of Bacterial Outer Membrane Projections And Associated Protein Complexes Using Electron Cryo-Tomography, Mohammed Kaplan, Georges Chreifi, Lauren Ann Metskas, Janine Liedtke, Cecily R. Wood, Catherine M. Oikonomou, William J. Nicolas, Poorna Subramanian, Lori A. Zacharoff, Yuhang Wang, Yi-Wei Chang, Morgan Beeby, Megan J. Dobro, Yongtao Zhu, Mark J. Mcbride, Ariane Briegel, Carrie L. Shaffer, Grant J. Jensen

Veterinary Science Faculty Publications

The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs …

Power Factor And Reactive Power In Us Residences – Survey And Energyplus Modeling, Hope C. Anderson, Abdullah Al Hadi, Evan S. Jones, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Electric power systems are experiencing a growing number of electronic loads in the residential sector as modern appliance technology progresses, and it has become increasingly more important to consider the total power factor (PF) of residential communities. This paper provides a survey based on literature and publicly available information of typical appliance PF values and effects at the residential level as well as a discussion on appliance energy use and corresponding operation schedules. A procedure for the calculation of equivalent PF is proposed and exemplified with minutely experimental data at 15-minute and hourly time intervals, which correspond to smart metering …

Analysis Of Traffic Crash Data In Kentucky 2016-2020, Eric Green, Paul Ross, Christopher Blackden, Michael Fields, William Staats

Kentucky Transportation Center Research Report

This report documents analysis of traffic crash data in Kentucky. A primary objective of this study was to determine average crash statistics for Kentucky highways. Where used, rates were calculated for various highway types and for counties and cities. Difference criteria were used for exposure.

Average and critical numbers, SPFs, and crash rates were calculated for various highway types in rural and urban areas. These metrics rely on crashes identified on highways where Annual Average Daily Traffic (AADT) volumes were available. Data in this report may be used to help identify problem areas.

The other primary objective of this study …

Aqueous Photochemistry Of 2-Oxocarboxylic Acids: Evidence, Mechanisms, And Atmospheric Impact, Marcelo I. Guzman, Alexis J. Eugene

Chemistry Faculty Publications

Atmospheric organic aerosols play a major role in climate, demanding a better understanding of their formation mechanisms by contributing multiphase chemical reactions with the participation of water. The sunlight driven aqueous photochemistry of small 2-oxocarboxylic acids is a potential major source of organic aerosol, which prompted the investigations into the mechanisms of glyoxylic acid and pyruvic acid photochemistry reviewed here. While 2-oxocarboxylic acids can be contained or directly created in the particles, the majorities of these abundant and available molecules are in the gas phase and must first undergo the surface uptake process to react in, and on the surface, …

High Depth-Of-Discharge Zinc Rechargeability Enabled By A Self-Assembled Polymeric Coating, David J. Arnot, Matthew B. Lim, Nelson S. Bell, Noah B. Schorr, Ryan C. Hill, Andrew Meyer, Yang-Tse Cheng, Timothy N. Lambert

Chemical and Materials Engineering Faculty Publications

Zinc has the potential for widespread use as an environmentally friendly and cost-effective anode material pending the resolution of rechargeability issues caused by active material loss and shape change. Here, a self-assembled Nafion-coated Celgard 3501 (NC-Celgard) separator is shown to enable unprecedented cycle life of a Zn anode in alkaline electrolyte at high depth-of-discharge (DOD_Zn). Using commercially relevant energy-dense electrodes with high areal capacities of 60 mAh cm^–2, Zn–Ni cells tested at 20% DOD_Zn cells achieve over 200 cycles while 50% DOD_Zn cells achieve over 100 cycles before failure. The 20% and 50% DOD …

Intravesical Cd74 And Cxcr4, Macrophage Migration Inhibitory Factor (Mif) Receptors, Mediate Bladder Pain, Shaojing Ye, Fei Ma, Dlovan F. D. Mahmood, Katherine L. Meyer-Siegler, Raymond E. Menard, David E. Hunt, Lin Leng, Richard Bucala, Pedro L. Vera

Physiology Faculty Publications

BACKGROUND: Activation of intravesical protease activated receptor 4 (PAR4) leads to release of urothelial macrophage migration inhibitory factor (MIF). MIF then binds to urothelial MIF receptors to release urothelial high mobility group box-1 (HMGB1) and elicit bladder hyperalgesia. Since MIF binds to multiple receptors, we investigated the contribution of individual urothelial MIF receptors to PAR4-induced HMGB1 release in vivo and in vitro and bladder pain in vivo.

METHODOLOGY/PRINCIPAL FINDINGS: We tested the effect of intravesical pre-treatment with individual MIF or MIF receptor (CD74, CXCR4, CXCR2) antagonists on PAR4-induced HMGB1 release in vivo (female C57/BL6 mice) and in vitro (primary …

Production Of Orchard-Grass Cultivars (Dactylis Glomerata) In Mixtures With Alfalfa (Medicago Sativa), Under Grazing Conditions, R. A. Barbarossa, D. P. Miñón

IGC Proceedings (1977-2023)

In the Lower Valley of the Río Negro (northern Patagonia, Argentina), six binary mixtures of orchard-grass (Dactylis glomerata) cultivars (Porto, Currie, Filox, a Local ecotype, Pergamino El Cencerro MAG) and alfalfa (Medicago sativa) Monarca SP INTA, were tested under grazing and irrigation. Mixtures were evaluated during three growing seasons (96/97; 97/98 and 98/99). Total forage production of mixtures and botanical composition was measured (orchard-grass cultivars and alfalfa). Porto, Currie, Filox, Local and Cencerro cultivars reached similar yield, while Amba produced less forage (p< 0.05). Mixture yield showed no significant difference, except Porto + alfalfa, which was lower (p< 0.05). The grass was higher in spring (1^st grazing) of three growing seasons. Orchard-grass + alfalfa showed highest dry matter …

Short-Term Transport Stress And Supplementation Alter Immune Function In Aged Horses, Ashton B. Miller, Patricia A. Harris, Virginia D. Barker, Amanda A. Adams

Veterinary Science Faculty Publications

Long-distance transport is associated with stress-related changes in equine immune function, and shipping-associated illnesses are often reported. Horses are frequently transported short distances, yet the effects of short-term transport on immune function remain largely unknown. Twelve horses, aged 15–30 yr, were assigned to either the control (n = 6) or treatment (n = 6) groups; treatment horses received a daily antioxidant supplement 3 weeks before and after transport. All horses were transported for approximately 1.5–2 hr on Day 0. Blood was collected via jugular venipuncture at 15-min pre- and post-transport and on Days –21, 1, 3, 7, 14, and 21. …

Innate Immune Activation By Checkpoint Inhibition In Human Patient-Derived Lung Cancer Tissues, Teresa W. M. Fan, Richard M. Higashi, Huan Song, Saeed Daneshmandi, Angela L. Mahan, Matthew S. Purdom, Therese J. Bocklage, Thomas A. Pittman, Daheng He, Chi Wang, Andrew N. Lane

Center for Environmental and Systems Biochemistry Faculty Publications

Although Pembrolizumab-based immunotherapy has significantly improved lung cancer patient survival, many patients show variable efficacy and resistance development. A better understanding of the drug’s action is needed to improve patient outcomes. Functional heterogeneity of the tumor microenvironment (TME) is crucial to modulating drug resistance; understanding of individual patients’ TME that impacts drug response is hampered by lack of appropriate models. Lung organotypic tissue slice cultures (OTC) with patients’ native TME procured from primary and brain-metastasized (BM) non-small cell lung cancer (NSCLC) patients were treated with Pembrolizumab and/or beta-glucan (WGP, an innate immune activator). Metabolic tracing with ¹³C₆-Glc/ …

Utilizing Patient-Derived Epithelial Ovarian Cancer Tumor Organoids To Predict Carboplatin Resistance, Justin W. Gorski, Zhuwei Zhang, J. Robert Mccorkle, Jodi M. Dejohn, Chi Wang, Rachel W. Miller, Holly H. Gallion, Charles S. Dietrich Iii, Frederick R. Ueland, Jill M. Kolesar

Obstetrics and Gynecology Faculty Publications

The development of patient-derived tumor organoids (TOs) from an epithelial ovarian cancer tumor obtained at the time of primary or interval debulking surgery has the potential to play an important role in precision medicine. Here, we utilized TOs to test front-line chemotherapy sensitivity and to investigate genomic drivers of carboplatin resistance. We developed six high-grade, serous epithelial ovarian cancer tumor organoid lines from tissue obtained during debulking surgery (two neoadjuvant-carboplatin-exposed and four chemo-naïve). Each organoid line was screened for sensitivity to carboplatin at four different doses (100, 10, 1, and 0.1 µM). Cell viability curves and resultant EC₅₀ values …

Participatory Identification Of Factors Inhibiting Integrated Management Of Livestock And Wildlife Production On Kenyan Rangelands, E. N. Muthiani, S. K. Karimi

IGC Proceedings (1977-2023)

No abstract provided.

Resilience-Focused Hiv Care To Promote Psychological Well-Being During Covid-19 And Other Catastrophes, L. Lauren Brown, Erika G. Martin, Hannah K. Knudsen, Heather J. Gotham, Bryan R. Garner

Behavioral Science Faculty Publications

The COVID-19 pandemic has adversely affected people with HIV due to disruptions in prevention and care services, economic impacts, and social isolation. These stressors have contributed to worse physical health, HIV treatment outcomes, and psychological wellness. Psychological sequelae associated with COVID-19 threaten the overall well-being of people with HIV and efforts to end the HIV epidemic. Resilience is a known mediator of health disparities and can improve psychological wellness and behavioral health outcomes along the HIV Continuum of Care. Though resilience is often organically developed in individuals as a result of overcoming adversity, it may be fostered through multi-level internal …

Intended And Unintended Effects Of E-Cigarette Taxes On Youth Tobacco Use, Rahi Abouk, Charles J. Courtemanche, Dhaval Dave, Bo Feng, Abigail S. Friedman, Johanna Catherine Maclean, Michael F. Pesko, Joseph J. Sabia, Samuel Safford

Institute for the Study of Free Enterprise Working Papers

Over the past decade, rising youth use of e-cigarettes and other electronic nicotine delivery systems (ENDS) has prompted aggressive regulation by state and local governments. Between 2010 and 2019, ten states and two large counties adopted ENDS taxes. Applying a continuous treatment difference-in-differences approach to data from two large national datasets (Monitoring the Future and the Youth Risk Behavior Surveillance System), this study explores the impact of ENDS taxes on youth tobacco use. We find that ENDS taxes reduce youth e-cigarette consumption, with estimated e-cigarette tax elasticities of -0.06 to -0.21. However, we estimate sizable positive cigarette cross-tax elasticities, suggesting …

Modification Of Cell Wall Polysaccharide Guides Cell Division In Streptococcus Mutans., Svetlana Zamakhaeva, Catherine T Chaton, Jeffrey S. Rush, Sowmya Ajay Castro, Cameron W Kenner, Alexander E Yarawsky, Andrew B Herr, Nina M Van Sorge, Helge C Dorfmueller, Gregory I Frolenkov, Konstantin V Korotkov, Natalia Korotkova

Molecular and Cellular Biochemistry Faculty Publications

In ovoid-shaped, Gram-positive bacteria, MapZ guides FtsZ-ring positioning at cell equators. The cell wall of the ovococcus Streptococcus mutans contains peptidoglycan decorated with serotype c carbohydrates (SCCs). In the present study, we identify the major cell separation autolysin AtlA as an SCC-binding protein. AtlA binding to SCC is attenuated by the glycerol phosphate (GroP) modification. Using fluorescently labeled AtlA constructs, we mapped SCC distribution on the streptococcal surface, revealing enrichment of GroP-deficient immature SCCs at the cell poles and equators. The immature SCCs co-localize with MapZ at the equatorial rings throughout the cell cycle. In GroP-deficient mutants, AtlA is mislocalized, …

Carta De Jamaica: Argumentación Y Cohesión Discursiva Desde La Perspectiva Del Análisis Histórico Del Discurso Y La Lingüística De Corpus, Yanira B. Paz, Juncos Alicia

Hispanic Studies Faculty Publications

El objeto de este estudio es deconstruir la estructura argumentativa de la llamada Carta de Jamaica, un texto considerado como visionario en el imaginario histórico, político y cultural latinoamericano. Para este fin se empleó la perspectiva combinada del análisis histórico del discurso (AHD) y de la lingüística de corpus (LC). Dentro del AHD se analizaron los aspectos formales y funcionales del proceso argumentativo en un esquema integrado, destacando la puesta a prueba de la validez de la declaración a partir de los actos de habla que lo conforman. Asimismo, se estudió la función de los marcadores del discurso como …

Interprofessional Inconsistencies In The Diagnosis Of Shoulder Instability: Survey Results Of Physicians And Rehabilitation Providers, Constantine P. Nicolozakes, Xinning Li, Timothy L. Uhl, Guido Marra, Nitin B. Jain, Eric J. Perreault, Amee L. Seitz

Physical Therapy Faculty Publications

Background: Clinicians of many specialties within sports medicine care for athletes with shoulder instability, but successful outcomes are inconsistent. Consistency across specialties in the diagnosis of shoulder instability is critical for care of the athlete, yet the extent of divergence in its diagnosis is unknown.

Hypothesis: Physicians differ from rehabilitation providers in which findings they deem clinically important to differentiate shoulder instability from impingement, and in how they diagnose athlete scenarios with atraumatic shoulder instability.

Study Design: Cross-sectional study.

Methods: Physicians (orthopaedic surgeons, primary care sports medicine physicians) and rehabilitation providers (physical therapists, athletic trainers) were asked via an online …

Recent Innovations In Education‐Extension‐Research Networks Of Industrialised Agricultures, Mark Paine, Marianne Cerf

IGC Proceedings (1977-2023)

No abstract provided.

Stimulating Market Led Livestock Production Through Information Communication Technologies: Experiences From Pastoral Areas Of Eastern Africa, R. Kaitho, G. Kariuki, E. Kebebe, J. Gutta, J. Ndungu, M. Kingamkono, P. Dyke

IGC Proceedings (1977-2023)

No abstract provided.

How Students And Principals Understand Classdojo: Emerging Insights, Daniela Kruel Digiacomo, Spencer P. Greenhalgh, Sarah C. Barriage

Information Science Faculty Publications

ClassDojo is a classroom communication and behavior management app intended to “bring every family into [the] classroom” (www.classdojo.com). The features of the platform include a points system to facilitate classroom management, instant teacher-parent communication (on the individual or class level), and student portfolios (among others). While ClassDojo claims to be used in over 95% of schools in the United States, there is little known about how students or principals interact with and understand the platform’s features and data. Drawing upon a mixed-methods study in a small state in the Southeastern United States, this article offers empirically driven insight …

Fully Coupled Internal Radiative Heat Transfer For The 3d Material Response Of Heat Shield, Raghava S. C. Davuluri, Rui Fu, Kaveh A. Tagavi, Alexandre Martin

Mechanical Engineering Faculty Publications

The radiative transfer equation (RTE) is strongly coupled to the material response code KATS. A P-1 approximation model of RTE is used to account for radiation heat transfer within the material. First, the verification of the RTE model is performed by comparing the numerical and analytical solutions. Next, the coupling scheme is validated by comparing the temperature profiles of pure conduction and conduction coupled with radiative emission. The validation study is conducted on Marschall et al. cases (radiant heating, arc-jet heating, and space shuttle entry), 3D Block, 2D IsoQ sample, and Stardust Return Capsule. The validation results agree well for …

Food Supply Chains In Developed Countries And Livestock Production Systems, Alan Mcdermott

IGC Proceedings (1977-2023)

No abstract provided.

Review Of: Understanding And Dismantling Racism: Crowdsourcing A Pathway Model In Appalachia, Matthew F. Hudson

Journal of Appalachian Health

The Journal of Appalachian Health is committed to reviewing published media that relates to contemporary concepts affecting the health of Appalachia. Examining Institutional Racism’s impact on health, career advancement and outcomes in Appalachian communities, impacts our ability to address and identify solutions to inform the fundamental framing of health equity. Dr. Matthew F. Hudson critiques the website: Understanding and Dismantling Racism: Crowdsourcing a Pathway Model in Appalachia.

Review Of: Appalachians For Medicaid Website, Kendra Barker Dnp

Journal of Appalachian Health

The Journal of Appalachian Health is committed to reviewing published media that relates to contemporary concepts affecting the health of Appalachia. Access to care and the health disparities we face have a direct effect on our experience of illness. Dr. Kendra Barker reviews the website: Appalachians for Medicaid.

Establishing Peer Recovery Support Services To Address The Central Appalachian Opioid Epidemic: The West Virginia Peers Enhancing Education, Recovery, And Survival (Wv Peers) Pilot Program, Stephen M. Davis, Amanda Stover, Herb Linn, Jon Dower, Dan Mccawley, Erin Winstanley, Judith Feinberg

Journal of Appalachian Health

Introduction: Central Appalachia has been disproportionately affected by the opioid epidemic and overdose fatalities. We developed West Virginia Peers Enhancing Education, Recovery, and Survival (WV PEERS), a program based on peer recovery support, to engage individuals using opioids and link them with a range of services.

Methods: Community partners providing services to individuals with opioid use disorder (OUD) were identified and collaborations were formalized using a standardized memorandum of understanding. The program was structured to offer ongoing peer recovery support specialist (PRSS) services, not just a one-time referral. A website and cards describing the WV PEERS program were developed and …

Emergence Of Covid-19 And Patterns Of Early Transmission In An Appalachian Sub-Region, Abbey K. Mann, Timothy A. Joyner, Ingrid E. Luffman, Megan Quinn, William Tollefson, Ashley Frazier

Journal of Appalachian Health

Background: In mid-March 2020, very few cases of COVID-19 had been confirmed in the Central Blue Ridge Region, an area in Appalachia that includes 47 jurisdictions across northeast Tennessee, western North Carolina, and southwest Virginia. Authors described the emergence of cases and outbreaks in the region between March 18 and June 11, 2020.

Methods: Data were collected from the health department websites of Tennessee, North Carolina, and Virginia beginning in mid-March for an ongoing set of COVID-19 monitoring projects, including a newsletter for local healthcare providers and a Geographic Information Systems (GIS) dashboard. In Fall 2020, using these databases, authors …

Appalachia: Putting The "Critical" In Race And Crowdsourcing A Pathway Model On Institutional Racism, Lauri Andress, Keri Valentine

Journal of Appalachian Health

As the website Understanding and Dismantling Racism: Crowdsourcing a Pathway Model in Appalachia explains, we are seeking assistance in refining a pathway model that elucidates institutional racism from the unique standpoint of Appalachia. We think that Appalachia has a distinctive cultural toolkit that shapes its orientation on issues. Our goal is to use crowdsourcing to harness this unique Appalachian ethos to refine the Pathway model on Institutional Racism based on comments, edits, questions, and ideas left on the website.

Math Predictors Of Numeric Health And Non-Health Decision-Making Problems, Clarissa A. Thompson, Jennifer M. Taber, Charles J. Fitzsimmons, Pooja G. Sidney

Psychology Faculty Publications

People frequently encounter numeric information in medical and health contexts. In this paper, we investigated the math factors that are associated with decision-making accuracy in health and non-health contexts. This is an important endeavor given that there is relatively little cross-talk between math cognition researchers and those studying health decision making. Ninety adults (M = 37 years; 86% White; 51% male) answered hypothetical health decision-making problems, and 93 adults (M = 36 years; 75% White; 42% males) answered a non-health decision-making problem. All participants were recruited from an online panel. Each participant completed a battery of tasks involving objective math …