Digital Commons Network^™

Exploring Perceived Importance Of A Novel Emergency Food Program During Covid-19 And Program Recipient Characteristics, Makenzie L. Barr, Kendra Oonorasak, Kristin Hughes, Lauren Batey, Kaela Jackson, Haley Marshall, Tammy Stephenson

Dietetics and Human Nutrition Faculty Publications

Following rising unemployment rates and consequent loss of income due to COVID-19, many people have been seeking meal assistance. This study examines the impact of a community-based free meal distribution program during the pandemic in Kentucky, reviewing characteristics of recipients of the program. Demographics, health behaviors, food insecure classification, and rating of importance of the meal program were collected. Qualitative feedback on the impact of the program was collected via open response. Of the 92 participants using the meal service, the cohort was female, Black, 43 years of age (43.5 ± 15.0 years), with a household income under 30,000 USD …

Recent Advances In Wearable Sensing Technologies, Alfredo J. Perez, Sherali Zeadally

Information Science Faculty Publications

Wearable sensing technologies are having a worldwide impact on the creation of novel business opportunities and application services that are benefiting the common citizen. By using these technologies, people have transformed the way they live, interact with each other and their surroundings, their daily routines, and how they monitor their health conditions. We review recent advances in the area of wearable sensing technologies, focusing on aspects such as sensor technologies, communication infrastructures, service infrastructures, security, and privacy. We also review the use of consumer wearables during the coronavirus disease 19 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus …

Biotechnological Improvement In Timothy Breeding, S. Pulli, Y. D. Guo

IGC Proceedings (1977-2023)

Biotechnological improvements in timothy breeding by androgenic and somatic cell culture and molecular identification were achieved. The doubled haploid (DH) plants were obtained via anther and microspore culture. A series of factors which affect androgenic embryogenesis and green plant regeneration have been evaluated. The PG-96 induction media which sharply promote androgenic embryogenesis have been established. Over 400 DH plants of timothy were obtained. Timothy somatic callus induction was successfully established using immature inflorescences and seedling stems. Suspension cultures were initiated from friable nodule-forming callus. More than 100 regenerated green plants were obtained from somatic callus and suspension clumps. DNA-based fingerprinting …

A Roadmap For Building Data Science Capacity For Health Discovery And Innovation In Africa, Joseph Beyene, Solomon W. Harrar, Mekibib Altaye, Tessema Astatkie, Tadesse Awoke, Ziv Shkedy, Tesfaye B. Mersha

Statistics Faculty Publications

Technological advances now make it possible to generate diverse, complex and varying sizes of data in a wide range of applications from business to engineering to medicine. In the health sciences, in particular, data are being produced at an unprecedented rate across the full spectrum of scientific inquiry spanning basic biology, clinical medicine, public health and health care systems. Leveraging these data can accelerate scientific advances, health discovery and innovations. However, data are just the raw material required to generate new knowledge, not knowledge on its own, as a pile of bricks would not be mistaken for a building. In …

Characterization Of Indoor Arenas Through An Anonymous Survey, Staci Mcgill, Morgan D. Hayes, Kimberly I. Tumlin, Robert Coleman

Biosystems and Agricultural Engineering Faculty Publications

Equine farms are building both stables for the horses to live in and additional facilities to train and work horses (Kidd et al., 1997). For many of these farms, an outdoor arena that has an all-weather footing is the first working facility built. During inclement weather the ability to train in the outdoor arenas is inhibited, which in turn means the trainers, riders, and farms lose income as money is only made when horses are working, training, and competing. Indoor arenas allow for horses to continue to be worked no matter the weather conditions. The equine industry contributes a total …

Effect Of Storage Time And Temperature On Recovery Of Synergistes Jonesii From Rumen Fluid And Feces, W. M. Andrew, M. J. Williams, M. J. Allison, C. C. Chase Jr., C. G. Chambliss, R. S. Kalmbacher, W. E. Kunkle

IGC Proceedings (1977-2023)

Synergistes jonesii is a rumen bacterium that degrades 3,4-dihydroxypyridine (3,4 DHP), the toxic breakdown product of mimosine in leucaena (Leucaena leucocephala). Fecal culture is the most practical way to determine S. jonesii presence in zoological ruminants, particularly if feces can be collected from night penning facilities. Fresh rumen fluid and fecal or fecal slurry (sheep [Ovis spp.] only, 1:4 wt to vol. feces and culture media) from cattle (Bos spp.) and sheep, known to be colonized by S. jonesii, were subjected various storage times (0, 6, 12, and 24 h) and temperatures (5, 23, and …

Cellular Origins Of Egfr-Driven Lung Cancer Cells Determine Sensitivity To Therapy, Fan Chen, Jinpeng Liu, Robert M. Flight, Kassandra J. Naughton, Alexsandr Lukyanchuk, Abigail R Edgin, Xiulong Song, Haikuo Zhang, Kwok-Kin Wong, Hunter N. B. Moseley, Chi Wang, Christine F. Brainson

Toxicology and Cancer Biology Faculty Publications

Targeting the epidermal growth factor receptor (EGFR) with tyrosine kinase inhibitors (TKIs) is one of the major precision medicine treatment options for lung adenocarcinoma. Due to common development of drug resistance to first- and second-generation TKIs, third-generation inhibitors, including osimertinib and rociletinib, have been developed. A model of EGFR-driven lung cancer and a method to develop tumors of distinct epigenetic states through 3D organotypic cultures are described here. It is discovered that activation of the EGFR T790M/L858R mutation in lung epithelial cells can drive lung cancers with alveolar or bronchiolar features, which can originate from alveolar type 2 (AT2) cells …

Extensive Livestock Production And Emerging Social Ethics For Animals, Bernard E. Rollin

IGC Proceedings (1977-2023)

The last 50 years have witnessed a dazzling array of social ethical revolutions in Western society. Such moral movements as feminism, civil rights, environmentalism, affirmative action, consumer advocacy, homosexual rights, children’s rights, the student movement, antiwar activism, public rejection of biotechnology, have forever changed the way governments and public institutions comport themselves. And this is equally true for private enterprise; to be successful, businesses must be seen as operating solidly in harmony with changing and emerging social ethics. It is arguable that morally based boycotting of South African business was instrumental in bringing about the end of apartheid, and similar …

Hindlimb Suspension In Wistar Rats: Sex-Based Differences In Muscle Response, Marie Mortreux, Megan E. Rosa-Caldwell, Ian D. Stiehl, Dong-Min Sung, Nicholas T. Thomas, Christopher S. Fry, Seward B. Rutkove

Athletic Training and Clinical Nutrition Faculty Publications

Ground-based animal models have been used extensively to understand the effects of microgravity on various physiological systems. Among them, hindlimb suspension (HLS), developed in 1979 in rats, remains the gold-standard and allows researchers to study the consequences of total unloading of the hind limbs while inducing a cephalic fluid shift. While this model has already brought valuable insights to space biology, few studies have directly compared functional decrements in the muscles of males and females during HLS. We exposed 28 adult Wistar rats (14 males and 14 females) to 14 days of HLS or normal loading (NL) to better assess …

Assessment Of Cultural Sensitivity In Dietetics Education, Julie Plasencia, Heather Norman-Burgdolf, Lorraine Weatherspoon

Dietetics and Human Nutrition Faculty Publications

Cultural sensitivity and competency are skills needed for agricultural professionals including nutrition and dietetics practitioners. The objective of the current study was to examine the learning transference of cultural sensitivity topics taught in a cultural foods course into case study assessments of a capstone-level course. This study is a cross-sectional, content analysis of cultural sensitivity assessment rubric (CSAR) scores for two case study assessments. The study was conducted in a landgrant, research-intensive university and 55 students (60%) from a capstone-level dietetics course participated. T-tests were used to compare CSAR scores between students who had completed a cultural foods course and …

Design Optimization And Experimental Study Of Coreless Axial-Flux Pm Machines With Wave Winding Pcb Stators, Peng Han, Damien Lawhorn, Yaser Chulaee, Donovin Lewis, Greg Heins, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

This paper presents a systematic design optimization and experimental study of coreless axial-flux permanent magnet (AFPM) machines with wave winding printed circuit board (PCB) stators. Existing PCB winding configurations are briefly reviewed and compared to serve as a performance reference. A new concept –the “macro coil”– is proposed to model PCB stator windings in 3D finite element analysis (FEA) to facilitate large-scale multi-objective optimization with the winding eddy current losses evaluated in each design candidate. The detailed trace-by-trace model for a selected optimal design is then built to check the voltage difference between parallel paths and the resulting circulating currents. …

Association Between Pitch Break On The 4-Seam Fastball And Slider And Shoulder Injury In Major League Baseball Pitchers: A Case-Control Study, Brooks N. Platt, Anthony J. Zacharias, Caitlin Conley, Lindsay Hockensmith, Nicholas Stockwell, Aaron Sciascia, Austin V. Stone

Orthopaedic Surgery and Sports Medicine Faculty Publications

Background: Few specific risk factors are known for shoulder injury in professional pitchers. New pitch-tracking data allow for risk stratification based on advanced metrics.

Purpose/Hypothesis: The purpose of this study was to determine the association between shoulder injury, pitch frequency, and pitch metrics (velocity, total break, break angle, and spin rate) for the 4-seam fastball, curveball, and slider. We hypothesized that more frequent use of the 4-seam fastball would be associated with shoulder injury.

Study Design: Case-control study; Level of evidence, 3.

Methods: The Major League Baseball (MLB) database was queried for pitchers who had been placed on the injury …

Design Optimization Of A Very High Power Density Motor With A Reluctance Rotor And A Modular Stator Having Pms And Toroidal Windings, Peng Han, Murat G. Kesgin, Dan M. Ionel, Rohan Gosalia, Nakul Shah, Charles J. Flynn, Chandra Sekhar Goli, Somasundaram Essakiappan, Madhav Manjrekar

Power and Energy Institute of Kentucky Faculty Publications

This paper proposes a new high power density permanent magnet (PM) motor design for traction applications to achieve the 50kW/L target set by the US Department of Energy by increasing the torque capability and operating speed compared to conventional PM machine topologies. A large-scale multi-objective design optimization based on 2D finite element analysis (FEA) and differential evolution algorithm was conducted to achieve the best trade-off among high efficiency, high power density and high power factor. The torque-speed envelopes are also checked for the Pareto front designs to make sure they have a constant power speed ratio of at least 3:1. …

Generalized Energy Storage Model-In-The-Loop Suitable For Energy Star And Cta-2045 Control Types, Huangjie Gong, Evan S. Jones, A. H. M. Jakaria, Aminul Huque, Ajit Renjit, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

The paper proposes a generalized energy storage (GES) model for battery energy storage systems (BESS), electric water heaters (EWH) and heating, ventilation, and air-conditioning (HVAC) systems. The analogies, including state of charge versus water temperature differential, are identified and explained, and models-in-the-loop (MIL) are introduced, which are compatible with the Energy Star and CTA-2045 general specifications and command types. Emphasis is placed on the proposed EWH model as it needfully fulfills a gap in present literature. The corresponding MIL has been implemented in the DER integration testbed, which was originally developed by EPRI, and satisfactorily validated against experimental results. A …

On The Modeling Of Bearing Voltage And Current In Pwm Converter-Fed Electric Machines Using Electromagnetic Finite Element Analysis, Peng Han, Yibin Zhang, Murat G. Kesgin, Greg Heins, Dean Patterson, Mark Thiele, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Bearing voltages and resulting currents in electric machines driven by PWM converters with fast switching and high dv/dt can cause premature bearing failures. With the transition from conventional Si devices to wide bandgap (WBG) devices and increase in switching frequency, bearing voltages and currents become more significant and need to be addressed from the early design stage. This paper proposes to use coupled field-circuit electromagnetic finite element analysis (FEA) to model bearing voltage and current in electric machines, which takes into account the influence of distributed winding conductors and frequency-dependent winding RL parameters. The three known bearing current types are …

Article: Anti- Love Jihad Law: An Analysis Of Women’S Religious And Marital Rights, Snehal Sharma

Gender and Women's Studies Faculty Publications

In November 2020, Uttar Pradesh government enacted an ordinance – ‘Prohibition of Unlawful Religious Conversion Ordinance, 2020’. Since the ordinance has made the process of religious conversion and interfaith marriage complicated and prolonged in the state, it is commonly known as ‘anti- love jihad law’. This paper analyses and lays out the potential impact of this ordinance on inter-religious marriages, and how it conflicts with women’s religious, marital, and bodily autonomy. Here the author discusses the procedures of anti-conversion ordinance along with the Special Marriage Act (1954) and personal laws related to marriage to assess their impact on inter-religious couples …

Cross-Sectional Association Of Toxoplasma Gondii Exposure With Bmi And Diet In Us Adults, Joel Cuffey, Christopher A. Lepczyk, Shuoli Zhao, Nicholas M. Fountain-Jones

Agricultural Economics Faculty Publications

Toxoplasmosis gondii exposure has been linked to increased impulsivity and risky behaviors, which has implications for eating behavior. Impulsivity and risk tolerance is known to be related with worse diets and a higher chance of obesity. There is little known, however, about the independent link between Toxoplasma gondii (T. gondii) exposure and diet-related outcomes. Using linear and quantile regression, we estimated the relationship between T. gondii exposure and BMI, total energy intake (kcal), and diet quality as measured by the Health Eating Index-2015 (HEI) among 9,853 adults from the 2009–2014 National Health and Nutrition Examination Survey. …

Supporting And Retaining Early Career Mathematics Teachers Using An Online Community Of Practice, Paula Jakopovic, Travis Weiland, Maria Campitelli, Lorraine Males, Lisa Amick

Science, Technology, Engineering, and Mathematics (STEM) Education Faculty Publications

This study reports on efforts over several years to design and implement a yearlong intervention intended to support secondary mathematics teachers in their early years of teaching. The intervention is designed to retain early career mathematics teachers in the professions by engaging them in the development of meaningful professional relationships with a school-based mentors, and by creating an online community of practice for support with other professionals. The intervention itself consists of early career teachers and their mentors participating in monthly professional learning activities such as online meetings, videoconferencing panels with experts, and collaborative reading and discussing timely, purposeful, and …

No Effect Of Hypercholesterolemia On Elastase-Induced Experimental Abdominal Aortic Aneurysm Progression, Toru Ikezoe, Takahiro Shoji, Jia Guo, Fanru Shen, Hong S. Lu, Alan Daugherty, Masao Nunokawa, Hiroshi Kubota, Masaaki Miyata, Baohui Xu, Ronald L. Dalman

Saha Cardiovascular Research Center Faculty Publications

Objective: Epidemiological studies link hyperlipidemia with increased risk for abdominal aortic aneurysms (AAAs). However, the influence of lipid-lowering drugs statins on prevalence and progression of clinical and experimental AAAs varies between reports, engendering controversy on the association of hyperlipidemia with AAA disease. This study investigated the impact of hypercholesterolemia on elastase-induced experimental AAAs in mice. Methods: Both spontaneous (targeted deletion of apolipoprotein E) and induced mouse hypercholesterolemia models were employed. In male wild type (WT) C57BL/6J mice, hypercholesterolemia was induced via intraperitoneal injection of an adeno-associated virus (AAV) encoding a gain-of-function proprotein convertase subtilisin/kexin type 9 mutation (PCSK9) followed by …

Bacterial Biofilm Growth On Various Dental Stabilization Systems For Avulsed And Luxated Teeth, Mahmoud Mona, Clay Walker, Luciana M. Shaddox, Roberta Pileggi

Oral Health Practice Faculty Publications

With the increased incidence of traumatic injuries and the advanced understanding of the periodontal and alveolar healing process, teeth splinting has become a common practice for stabilizing traumatized teeth. Consequently, several splinting materials and techniques have been introduced in the past few years. Despite the detrimental role of bacterial biofilm on healing, the level of biofilm development on these material surfaces has not been well investigated. Bacterial biofilms are severely detrimental for periodontal healing of avulsed and luxated teeth. Thus, biofilm growth becomes a critical factor in selecting the material of choice for dental splints. In this study, we aim …

Association Of Manganese Biomarker Concentrations With Blood Pressure And Kidney Parameters Among Healthy Adolescents: Nhanes 2013–2018, Maria D. Politis, Jacob C. Freedman, Erin N. Haynes, Alison P. Sanders

Epidemiology and Environmental Health Faculty Publications

Deficiency or excess exposure to manganese (Mn), an essential mineral, may have potentially adverse health effects. The kidneys are a major organ of Mn site-specific toxicity because of their unique role in filtration, metabolism, and excretion of xenobiotics. We hypothesized that Mn concentrations were associated with poorer blood pressure (BP) and kidney parameters such as estimated glomerular filtration rate (eGFR), blood urea nitrogen (BUN), and albumin creatinine ratio (ACR). We conducted a cross-sectional analysis of 1931 healthy U.S. adolescents aged 12–19 years participating in National Health and Nutrition Examination Survey cycles 2013–2014, 2015–2016, and 2017–2018. Blood and urine Mn concentrations …

Co-Carriage Of Metal And Antibiotic Resistance Genes In Sewage Associated Staphylococci, Atena Amirsoleimani, Gail M. Brion, Patrice Francois

Civil Engineering Faculty Publications

Controlling spread of resistance genes from wastewater to aquatic systems requires more knowledge on how resistance genes are acquired and transmitted. Whole genomic sequences from sewage-associated staphylococcus isolates (20 S. aureus, 2 Staphylococcus warneri, and 2 Staphylococcus delphini) were analyzed for the presence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs). Plasmid sequences were identified in each isolate to investigate co-carriage of ARGs and MRGs within. BLASTN analysis showed that 67% of the isolates carried more than one ARG. The carriage of multiple plasmids was observed more in CC5 than CC8 S. aureus strains. Plasmid …

Neuron-Derived Extracellular Vesicles Modulate Microglia Activation And Function, Hui Peng, Brock T. Harvey, Christopher I. Richards, Kimberly Nixon

Pharmaceutical Sciences Faculty Publications

Microglia act as the immune cells of the central nervous system (CNS). They play an important role in maintaining brain homeostasis but also in mediating neuroimmune responses to insult. The interactions between neurons and microglia represent a key process for neuroimmune regulation and subsequent effects on CNS integrity. However, the molecular mechanisms of neuron-glia communication in regulating microglia function are not fully understood. One recently described means of this intercellular communication is via nano-sized extracellular vesicles (EVs) that transfer a large diversity of molecules between neurons and microglia, such as proteins, lipids, and nucleic acids. To determine the effects of …

“We Just Need The Developer To Develop”: Entrepreneurialism, Financialization And Urban Redevelopment In Lexington, Kentucky, Kevin Ward, Andrew Wood

Geography Faculty Publications

Since the 1980s US city governments have increased their use of more speculative means of financing economic redevelopment. This has involved experimenting with a variety of financial and taxation instruments as a way of growing their economies and redeveloping their built environments. This very general tendency, of course, masks how some cities have done well through the use of these instruments while others have not. The work to date has tended to pivot around a “winner-loser dichotomy”, which emphasises either the capacity of US cities to be able to experiment and speculate through the use of one financial instrument or …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

1. Advancing Prediction of Headwater Flow Permanence and Stream Expansion and Contraction Using a Process-Based Hydrologic Model
2. Developing ANN Model for Predicting Lake Michigan E.Coli Counts
3. Examining Long Term Trends in Rainfall and Stream Flow at Upper Wabash River Basin Using Self Organizing Map
4. Investigating Water and Sediment Transport Processes with High-Resolution Sensor Measurements and Hysteresis Analysis in the Cane Run Royal Spring Basin, Kentucky, USA
5. Blue Water Farms: Edge-of-Field Water Quality Monitoring of Nutrient and Sediment Loss from No-Till Corn and Soybean Fields in the Lower Cumberland River Watershed
6. Determination of Microcystin Cyanobacterial Toxins in Kentucky Lakes by Diffusive Gradients …

Development Of Predictive Equations For Thermal Conductivity Of Compost Bedding, Flávio A. Damasceno, Joseph L. Taraba, George B. Day V, Randi A. Black, Jeffrey M. Bewley, Tales J. Fernandes, Carlos E. A. Oliveira, Rafaella R. Andrade, Matteo Barbari, Patrícia F. P. Ferraz, Lorenzo Leso

Biosystems and Agricultural Engineering Faculty Publications

Among animal facilities, compost-bedded pack (CBP) barns have attracted a lot of attention from milk producers and the scientific community. Systematic investigation of the main thermal properties utilizing sawdust in CBP barns is of environmental and economic relevance. In this paper, the aim was to (a) develop predictive equations for the thermal conductivity (k) of compost bedding as a function of moisture content (MC), the degree of compaction (DC_o), and particle size (PS); and (b) investigate the links between k and depth within bedding material. Samples of compost bedding materials were collected from 42 commercial CBP …

Prediction Of Herbage Accumulation Of Cynodon Grasses By An Empirical Model Based On Temperature And Daylength, H. R. De Medeiros, Carlos G. S. Pedreira, N. A. Villa Nova, L. G. Barioni, A. C. C. Mello

IGC Proceedings (1977-2023)

The objective of this work was to estimate dry matter production of Cynodon grasses as a function of photothermal units (PU). Total PU values were calculated for the period from February 1 to April 24, 1996, from a location in southeastern Brazil. Regression analysis was run between PU and herbage production from a published data set on five Cynodon cultivars, with an r² of 0.9983. Differences between measured and estimated values were under 5%. Model testing using an independent data set on herbage dry matter production from grazed Cynodon pastures showed an overestimation, probably due to water deficit during …

Smartphone Use And Psychological Well-Being Among College Students In China: A Qualitative Assessment, Cheng Dai, Zixue Tai, Shan Ni

Journalism and Media Faculty Publications

Background: Problematic smartphone use is widespread, and college-age youth faces an especially high risk of its associated consequences. While a promising body of research has emerged in recent years in this area, the domination of quantitative inquiries can be fruitfully and conceptually complemented by perspectives informed through qualitative research. Toward that end, this study aimed to interrogate the myriad behavioral, attitudinal, and psychological tendencies as a side effect of college students’ engagement with the smartphone in their everyday lived experience through in-depth interviews.

Methods: We recruited 70 participants from seven college campuses hailing from different geographic regions in China, and …

Real-World Evaluation Of Universal Germline Screening For Cancer Treatment-Relevant Pharmacogenes, Megan L. Hutchcraft, Nan Lin, Shulin Zhang, Catherine Sears, Kyle Zacholski, Elizabeth A. Belcher, Eric B. Durbin, John L. Villano, Michael J. Cavnar, Susanne M. Arnold, Frederick R. Ueland, Jill M. Kolesar

Pathology and Laboratory Medicine Faculty Publications

The purpose of this study was to determine the frequency of clinically actionable treatment-relevant germline pharmacogenomic variants in patients with cancer and assess the real-world clinical utility of universal screening using whole-exome sequencing in this population. Cancer patients underwent research-grade germline whole-exome sequencing as a component of sequencing for somatic variants. Analysis in a clinical bioinformatics pipeline identified clinically actionable pharmacogenomic variants. Clinical Pharmacogenetics Implementation Consortium guidelines defined clinical actionability. We assessed clinical utility by reviewing electronic health records to determine the frequency of patients receiving pharmacogenomically actionable anti-cancer agents and associated outcomes. This observational study evaluated 291 patients with …

Apoε4 Lowers Energy Expenditure In Females And Impairs Glucose Oxidation By Increasing Flux Through Aerobic Glycolysis, Brandon C. Farmer, Holden C. Williams, Nicholas A. Devanney, Margaret A. Piron, Grant K. Nation, David J. Carter, Adeline E. Walsh, Rebika Khanal, Lyndsay E. A. Young, Jude C. Kluemper, Gabriela Hernandez, Elizabeth J. Allenger, Rachel Mooney, Lesley R. Golden, Cathryn T. Smith, J. Anthony Brandon, Vedant A. Gupta, Philip A. Kern, Matthew S. Gentry, Josh M. Morganti, Ramon C. Sun, Lance A. Johnson

Physiology Faculty Publications

BACKGROUND: Cerebral glucose hypometabolism is consistently observed in individuals with Alzheimer's disease (AD), as well as in young cognitively normal carriers of the Ε4 allele of Apolipoprotein E (APOE), the strongest genetic predictor of late-onset AD. While this clinical feature has been described for over two decades, the mechanism underlying these changes in cerebral glucose metabolism remains a critical knowledge gap in the field.

METHODS: Here, we undertook a multi-omic approach by combining single-cell RNA sequencing (scRNAseq) and stable isotope resolved metabolomics (SIRM) to define a metabolic rewiring across astrocytes, brain tissue, mice, and human subjects expressing APOE4.

RESULTS: Single-cell …