Digital Commons Network^™

Black Youth Are Suffering In Silence: Advancing Social Work Education To Better Address The Mental Health Of African American Youth., Alicia Dawson

DSW Capstone Projects

African American youth’s rates of major depression, generalized anxiety disorder, post-traumatic stress disorder, and suicide have increased in the last three years due to COVID-19 and national social injustices. African American students who seek help at school are less likely to receive adequate treatment from school social workers. Mental health care for African American students should be available, and those services must be effective. African American youth continue to suffer in silence when school social workers lack African-centered conceptual teaching and therapeutic skills. Current social work education curriculums contribute to 'Whiteness,' which influences future social workers to carry biases and …

Oxidative Damage To Brain Cells Underlies: (I) Resistance To Radiation And Increased Tumor Cell Growth In Glioblastoma; (Ii) Apoe Allele Status And Pentose Phosphate Pathway Proteins In Alzheimer Disease Mouse Models, Nicole Rummel

Theses and Dissertations--Chemistry

Oxidative phosphorylation occurs within the inner mitochondrial membrane producing ATP for the cell’s energy needs. The Electron Transport Chain carries out the transfer of electrons from electron carriers through a series of proteins to form a proton gradient across the inner mitochondrial membrane. This gradient acts as the energy source needed to put a phosphate onto ADP and form ATP. With the large amounts of free electrons and oxygen within the mitochondria, an inevitable by-product of free radicals in the form of superoxide are produced. Superoxide (O^-̇₂) is a reactive radical that can react further to …

Synthesis Of 6,6- And 7,7-Difluoro-1-Acetamidopyrrolizidines And Their Oxidation Catalyzed By The Nonheme Fe Oxygenase Lolo, Nabin Panth

Theses and Dissertations--Chemistry

One of the remarkable steps in loline alkaloid biosynthesis is the installation of an ether bridge between two unactivated C atoms in 1-exo-acetamidopyrrolizidine (AcAP). LolO, a 2-oxoglutarate-dependent nonheme Fe oxygenase, catalyzes both the hydroxylation of AcAP and the resulting alcohol's cycloetherification to give N-acetylnornoline (NANL). The mechanism of hydroxylation is well understood, but the mechanism of the oxacyclization is not. I synthesized difluorinated analogs of AcAP in an attempt to further understand the mechanism of the unusual cycloetherification step.

I prepared 6,6-F₂-AcAP in eight steps from N,O-protected 4-oxoproline. The key step was a Dieckmann …

The Role Of Charge On Dna Packaging And Integrity Within Reconstituted Peptide-Dna Assemblies, Ehigbai Oikeh

Theses and Dissertations--Chemistry

In nature, DNA exists primarily in a highly compacted form. The compaction of DNA in vivo is mediated by cationic proteins; histone in somatic nuclei and arginine-rich peptides called protamines in sperm chromatin. The packaging in the sperm nucleus is significantly higher than somatic nuclei resulting in a final volume roughly 1/20^th that of a somatic nucleus. This tight packaging results in a near crystalline packaging of the DNA helices. While the dense packaging of DNA in sperm nuclei is considered essential for both efficient genetic delivery as well as DNA protection against damage by mutagens and oxidative species, …

Development Of Fluorescence Based Approaches To Understand Astrocyte Biology In The Context Of Nicotine And Nicotinic Receptor Activity, Surya P. Aryal

Theses and Dissertations--Chemistry

Smoking and tobacco use (STU) is a major global health problem and worldwide more than six million people die due to tobacco related diseases each year. Although majority of smokers try to quit smoking several times in their life, traditional therapeutic approaches, which focus only on neuronal cells, have a very low success rate. Understanding the effect of nicotine on glial cells, synaptic communication and blood vasculature in the brain can provide further insights on the neurobiology of substance abuse and can potentially help to design better therapeutic approaches. Glial cells are non-excitable cells in the brain which do not …

Application Of Mass Spectrometry For Characterization Of Plant-Based Phenolics And Alkaloids, Masoumeh Dorrani

Theses and Dissertations--Chemistry

Plant-derived compounds have the potential to produce value-added compounds with a variety of applications. For example, the lignin part of the lignocellulosic biomass, produced in large quantities as waste from the paper and pulp industries, is a rich source of phenolics with potential applications in the renewable energy sector, pharmaceutical, and chemical industries. On the other hand, plant alkaloids are the primary source for developing plant-derived therapeutics. Unfortunately, the recalcitrant nature of plant cell walls, low extraction yields of small secondary metabolites, and the lack of effective analytical methods for a rapid and accurate identification of plant-based compounds and plant’s …

Structural Basis Of Bacterial Flagellin For Naip5 Binding And Nlrc4 Inflammasome Activation And The Mechanism Of Flagellin Induced Release Of Cytokines In Vivo, Jian Cui

Theses and Dissertations--Chemistry

The bacterial flagellum is a whip-like structure that protrudes from the cell membrane and is one of the most complex and dynamic biological molecular machines that propels bacteria to swim toward beneficial environments and the sites of infection. It is composed of a basal body, a hook, and a long filament. The flagellar filament contains thousands of copies of the protein flagellin (FliC) monomer arranged helically and ending with a filament cap composed of oligomer protein FliD. The overall structure of the filament core is preserved across bacterial species, while the outer domains exhibit high variability, and in some cases …

Mechanism Of Antibiotic Permeability Through The Gram-Negative Bacterial Envelope, Olaniyi Alegun

Theses and Dissertations--Chemistry

The outer membrane of Gram-negative bacteria (GN) makes them distinct among superbugs that are associated with the development of antibiotic resistance. The outer membrane, and inner membrane, separated by the periplasm, form a double-membrane barrier to the entry of antibiotics into the cell. Several studies have been conducted to examine the role of outer membrane modifications such as porins, lipopolysaccharides, and efflux pumps on antibiotic resistance. However, there is a paucity of knowledge on how antibiotics behave in the periplasm, to gain access into their target region. My thesis focuses on understanding the mechanism of antibiotic permeability through the cellular …

Antibiotic Permeation In Gram-Negative Bacteria And Contribution Of Inflammasome Activation And Pyroptosis In Pathogenesis Of Salmonella Systemic Infection, Ankit Pandeya

Theses and Dissertations--Chemistry

Antibiotic resistance is one of the major global issues in the field of public health and medicine. Good antibiotic candidates need to be selectively toxic, inhibit cellular target, and effectively penetrate and accumulate in bacterial cells. The last factor is a formidable barrier in the development of antimicrobials effective in Gram-negative bacteria, due to the presence of two layers of cell envelope. The first half of my thesis focuses on understanding the permeation of small molecules through this formidable cell envelope, distribution inside the cell of Gram-negative bacteria, and design of novel methods to make small molecules effectively cross the …

Nicotine And Cotinine: Microbial Modulators Of The Immune Response, Jourdan Lakes

Theses and Dissertations--Chemistry

The consumption of tobacco products is a known contributor to a variety of health conditions and disease pathologies including cardiovascular and lung diseases. In addition to these well-known pathological effects of tobacco use, it has been established recently that tobacco consumption as well as nicotine consumption alone results in distinct, dysbiotic perturbations to the enteric microflora of the gastrointestinal tract. Previous research into the intestinal microbiome has demonstrated that these microbes are active contributors to the maintenance of a homeostatic and tolerogenic environment within the host. In fact, the presence of our enteric microflora is necessary for the proper development …

Succinylated Polyethylenimine Gene Delivery Agents For Enhanced Transfection Efficacy, Md. Nasir Uddin

Theses and Dissertations--Chemistry

Gene therapy aims to treat patients by altering or controlling gene expression. Today, most clinical approaches are viral-based due to their inherent gene delivery activity. However, there is still a significant interest in nonviral alternatives for gene delivery, particularly synthetic lipids and polymers, that do not suffer the immunogenicity, high cost, or mutagenesis concerns of viral vectors. Polymeric vectors are of particular interest due to the ability to further tune the polymer properties through the incorporation of additional functional units such as targeting ligands or shielding domains. Polyethylenimine (PEI), a highly cationic polymer, is often considered a benchmark for polymer-based …

Developments In Gold(Iii) Scaffolds For Protein Bioconjugation And Enhanced Anticancer Activity, Sailajah Gukathasan

Theses and Dissertations--Chemistry

Site-selective modifications of target proteins using specially designed small molecules is a powerful tool that has been extensively utilized in biomedicine. Small molecules can modify proteins either covalently or non-covalently depending on their structures and intrinsic chemical reactivity. Covalent chemical modification presents a more stable and often irreversible interaction with target proteins; unlike non-covalent binders, which form weak, reversible interactions with protein. Therefore, covalent modifiers represent an effective class of therapeutics due to their stability and irreversibility once bound to target proteins of interest. I hypothesized that tuning biocompatible, high-valent gold(III) complexes toward nucleophile-induced reductive elimination will lead to covalent …

The Design And Synthesis Of New Organic Dithiols For Environmental Applications, Shashika Nadishani Bandara

Theses and Dissertations--Chemistry

A thiol molecule, 2,6-pyridinediamidoethanethiol (PB9), was synthesized based on the pyridine-2,6-dicarboxamide scaffold with appended cysteamine groups. PB9 acts as an effective chelator for Pb(II) due to multiple binding sites (N₃S₂) through irreversible binding precipitating Pb(II). Removal of aqueous Pb(II) from solution was demonstrated by exploring the effects of time, initial PB9:Pb(II) ratios, pH, exposure time, and solution temperature. After 15 min the Pb(II) concentrations were reduced from 50.00 ppm to 0.30 ppm (99.4%) and 0.25 ppm (99.5%) for PB9:Pb ratios of 1:1 and 2:1, respectively. Removal of > 93% Pb(II) was observed over multiple pH values with …

1,2-Diamination Of Alkenes Via Reduction Of 1,2,3-Triazolinium Ions, Setareh Saryazdi

Theses and Dissertations--Chemistry

1,2-Diamine substructures are prevalent functional motifs in natural products, pharmaceutical compounds, and ligands. The interesting functionalities of 1,2-diamines have inspired many synthetic chemists to design various methodologies for preparing these structures from simple precursors such as alkenes. In this work, we described two different but related methods using simple and easily accessible reagents for 1,2-diamination of alkenes. In the first method, an alkene undergoes 1,3-dipolar cycloaddition with an organic azide to form a 1,2,3-triazoline. Subsequent N-alkylation of the generated 1,2,3-triazoline gives the 1,2,3-triazolinium ion, which is then hydrogenated over Raney Ni with a balloon of H₂ to produce …

The Role Of Organizational Leaders In Employee Self-Care: A Change Management Approach, Olivia Dawn Honaker

DSW Capstone Projects

Although literature demonstrates that helping professionals have had high levels of stress and burnout for decades, the COVID-19 pandemic has only exacerbated the issue. The already burdened healthcare workers, now facing increased workloads, long hours, and high-level exposure to trauma, have created an urgency to address this significant risk to helping professionals. The current capstone will bring awareness to organizational leaders regarding the importance of employee self-care and the benefits of implementing employee self-care programs. First, a systematic literature review will examine self-care in helping professions and explore how organizational leaders operationalize employee self-care programs. In addition, the capstone aims …

Bolstering Leadership Engagement To Community Coalition Work In Rural Appalachia, Melissa Slone

DSW Capstone Projects

Rural Appalachian communities are overwhelmed with high cancer rates, diabetes, disabilities, and substance use disorder. The challenges in addressing these increase when you factor in workforce shortages and limited funding. The literature documents the effectiveness of coalitions in addressing healthcare challenges within communities. The need is for leaders to reduce their hesitancy to engage in community coalition efforts and increase awareness of the impact community coalitions can have on addressing rural populations' health and well-being needs.

Product One was a systematic review focused on research that examined agency leaders’ perspectives on community coalition involvement. The purpose of conducting the systematic …

Healthcare Equity For Transgender Individuals, Mollie Staggs

DSW Capstone Projects

In the field of social justice and integrity, Social Work has dedicated itself to improving the welfare of the world, in which we all participate and interact. There are countless issues in our society that warrant professional attention for the fundamental purpose of positively impacting the livelihood of subcategories of people. For this particular Capstone Project, the focus is on transgender individuals’ systemic healthcare barriers that cisgender people within the United States do not similarly experience. The purpose is to unveil these disparities to then pose progressive alterations within the healthcare system to make the navigation and accessibility better for …

Children And High Conflict Divorce: Using Equine-Assisted Psychotherapy As Treatment, Ashley Berry

DSW Capstone Projects

The act of divorce has been commonplace throughout the world during the 20^th century, plateauing around the 1970s at around 50% of all marriages. While most divorces end peacefully, many contain ongoing conflict which can become detrimental to all involved, including the children. While historical treatment methods focus on changing the behavior of parents, there are instances where these methods may be ineffective, especially when the parents are unable or unwilling to change their conflictual behaviors. Equine-Assisted Psychotherapy (EAP), which has been used with individuals who have suffered from trauma, may be an option to use with this population …

Bolstering The Effectiveness Of Exposure-Response Prevention In The Treatment Of Obsessive-Compulsive Disorder: Using Self-Compassion As An Adjunctive Intervention, Whitney E. Dyer

DSW Capstone Projects

Individuals suffering with Obsessive-Compulsive Disorder (OCD) face many challenges receiving proper diagnostics and evidence-based treatment. Furthermore, Exposure-Response Prevention (ERP), the empirically supported gold standard approach for targeting OCD, presents its own challenges. Examining the influences of ERP treatment reluctance is paramount to bolstering the effectiveness of outcomes and safeguarding participant adherence and ultimate success. This Capstone highlights the overlooking of addressing shame within traditional ERP protocol and posits a practice application model that supports the adjunctive use of self-compassion, a potent antidote for shame, within the use of ERP. As treatment hesitation is an inhibitor to relief for OCD sufferers, …

Meta-Practice For The Bachelor Level: A Needed Compliment To The Generalist Model, Patrick Callahan

DSW Capstone Projects

Meta-practice in this concept is the web or roadmap, that is, the interconnectedness of the three pillars of the generalist model of social work. The generalist model teaches that micro, mezzo, and macro are interconnected. However, students and new professionals often do not understand precisely what the connection is. This work gathers the available literature, the conceptual idea of the introduction for the bachelor level of education, and an application of introducing it in a course for instruction. There is a need for introducing meta-practice at the bachelor’s level because of the continued globalization of society. By teaching the concept …

Neuroleadership A New Burnout Prevention Strategy, Zakery David Sloan

DSW Capstone Projects

This capstone project examines the current state of burnout, prevention strategies, prevalence, and introduces neuroleadership as a new leadership theory to advance burnout prevention among the public human service population. Neuroleadership presents a new conceptualization of leadership practice and burnout prevention method by utilizing neuro-informed leadership practices. This new neuro-informed leadership strategy is explored throughout the three major products of this capstone project including a systematic literature review, conceptual paper, and practice application paper.

The first product completed was a systematic literature review. This literature review included a total of 13 articles which were used to answer the research question, …

Defining Posttraumatic Stress In Law Enforcement: Normalizing Trauma Response, Britta D. Caudill

DSW Capstone Projects

Significant inequalities exist within the expectations of police officers regarding trauma exposure and the lack of mental health preventive efforts and treatment opportunities. The prevalence of stigma thrives within the thought process of this culture paired with the expectation for resiliency, as police culture focuses on the ability to repress emotion instead of utilizing appropriate coping methods. This review takes an interesting look at the history, definition, and diagnostic criteria of post-traumatic stress disorder (PTSD) by focusing on its evolution within the Diagnostic and Statistical Manual (DSM) over time. The primary goal is to establish a relationship between the definition …

Postpartum Depression: Mitigating Treatment Barriers For Women In Appalachia, Tabitha Simpson

DSW Capstone Projects

This capstone focuses on maternal mental needs, specifically postpartum care and postpartum depression. Each article in this capstone centers on postpartum depression, treatment barriers, introducing standard care of practice for women in rural communities, and identifying gaps leading to further research in postpartum education. Countless articles were read and utilized in conducting the systematic literature review to begin this process.

The first article in the capstone, the Systematic literature review, details the signs and symptoms of postpartum depression and how it differs from a major depressive disorder. A thorough review of the literature was conducted, identifying the central concern of …

Good Trouble: Disrupting The Disproportionality Of Black Boys In Special Education Due To Misdiagnosed And Unaddressed Trauma, Montrell Pryor

DSW Capstone Projects

Black boys experience a myriad of trauma as they navigate through society under the gaze of white patriarchy and archetypes. Whether engaging in trauma or witnessing it, it impacts Black boys tremendously, particularly in the educational setting. What typically looks like misbehavior in the classroom can be symptoms of unaddressed and untreated trauma that have been triggered and exacerbated in the school setting. As a result, this phenomenon contributes to the disproportionality of Black boys in special education and adds to the expansion of the school-to-prison pipeline.

This capstone provides a thorough examination of the literature in the form of …

Addressing The Mental Health Disparities Among Black Children During Natural Disasters: A Clinical Perspective And Response To The Covid-19 Pandemic., Brittany Reed

DSW Capstone Projects

The experiences of Black children resulting from the events surrounding the Covid-19 pandemic present a projection to create long-term mental health effects at higher rates than their peers of other races. While the long-term effects of this pandemic are still considered ongoing, the disparities within the Black community will subsequently develop into a trickle-down effect of secondary traumatic experiences faced by Black children. Then, where does that leave Black children and their mental health? This capstone project aims to examine how the Covid-19 pandemic will affect Black children, explore the mental health challenges and disparities faced by Black children resulting …

Sexual Orientation And Gender Expression As Predictors Of Sogie-Based Harassment, Michelle Tam

Theses and Dissertations--Psychology

The current study examined which individuals are most at risk for becoming targets of SOGIE-based harassment (specifically, White, male sexual minorities or White, male gender nonconforming individuals). The study also explored potential motivations behind SOGIE-based harassment (specifically, violations of normality and violations of morality) and whether these motivations are predicted by individual differences (specifically, sexual prejudice, beliefs in heteronormativity, tolerance of ambiguity, and adherence to gender norms). College students (n = 206; 67.5% female) were randomly assigned to one of four conditions, each with a different male target (straight/gender conforming, straight/gender nonconforming, gay/gender conforming, gay/gender nonconforming). Students saw a …

Early Maturing Out Of Problematic Alcohol Use, Sarah J. Peterson

Theses and Dissertations--Psychology

Most research suggests that alcohol use peaks in the college years then declines into the mid-thirties (Jochman & Fromme, 2010). However, there is evidence that some individuals mature out earlier: downward trends for some individuals begin in college, with as many as one third of students decreasing their drinking (Baer et al., 2001). It is crucial to identify factors that differentiate those who decrease their drinking early from those who persist in high levels of consumption; doing so would clarify risk for college-related alcohol problems and perhaps subsequent alcohol use disorder, and aid in earlier targeted prevention and intervention. This …

Mild Cognitive Impairment In Presurgical Deep Brain Stimulation For Parkinson's Disease, Elizabeth Roslyn Wallace

Theses and Dissertations--Psychology

Although clinically characterized by motor impairments, Parkinson's disease (PD) often affects cognition early in the disease course. Cognitive changes common in PD include visuospatial abnormalities and prominent executive function (EF) deficits, with 30% of individuals eventually developing Parkinson’s disease dementia (PDD). Mild cognitive impairment (MCI) has been identified as a transitional state between normal cognition and PDD. A large cohort of individuals with PD at the Kentucky Neuroscience Institute have undergone pre-surgical evaluations for deep brain stimulation, although cognitive performance in this cohort has never been probed. Baseline cognitive performance of this cohort from 2017-2020 was examined to characterize the …

Development And Cross-Validation Of Personality Assessment Inventory Decision Rules For The Identification Of Psychogenic Nonepileptic Seizures, Chelsea Marie Bosch

Theses and Dissertations--Psychology

The published literature on the Personality Assessment Inventory (PAI) for psychogenic nonepileptic seizure (PNES) diagnosis includes a variety of interpretation methods to distinguish PNES from epileptic seizures (ES) and offers mixed findings. The purpose of this study was to use a cross-validation approach to create and derive new decision rules for the PAI to best differentiate PNES from ES. Data from 773 patients (PNES n = 328, ES n = 445) who underwent long-term video EEG (vEEG) monitoring and completed a PAI were examined. Individuals with invalid PAI profiles were removed, and patients were randomly assigned to the “development” group …

Assessing The Temporal Relationship Between Changes In Neuroticism And Symptom Improvement In The Unified Protocol, Nicole Stumpp

Theses and Dissertations--Psychology

Neuroticism is defined as the tendency to experience frequent and intense negative emotions accompanied by the belief that one could not cope adequately in response to stress. Neuroticism is associated with the development and maintenance of a range of emotional disorders (e.g., anxiety disorders, depression) and targeting this trait in treatment (rather than symptoms) may represent a more efficient approach to care. However, researchers have rarely measured neuroticism and symptoms frequently enough to establish temporal precedence between these dimensions. The present study is a secondary analysis that examined the temporal relationship between neuroticism and anxiety and depressive symptoms during a …