Digital Commons Network^™

Second Heart Field–Derived Cells Contribute To Angiotensin Ii–Mediated Ascending Aortopathies, Hisashi Sawada, Yuriko Katsumata, Hideyuki Higashi, Chen Zhang, Yanming Li, Stephanie Morgan, Lang H. Lee, Sasha A. Singh, Jeff Z. Chen, Michael K. Franklin, Jessica J. Moorleghen, Deborah A. Howatt, Debra L. Rateri, Ying H. Shen, Scott A. Lemaire, Masanori Aikawa, Mark W. Majesky, Hong S. Lu, Alan Daugherty

Sanders-Brown Center on Aging Faculty Publications

BACKGROUND: The ascending aorta is a common location for aneurysm and dissection. This aortic region is populated by a mosaic of medial and adventitial cells that are embryonically derived from either the second heart field (SHF) or the cardiac neural crest. SHF-derived cells populate areas that coincide with the spatial specificity of thoracic aortopathies. The purpose of this study was to determine whether and how SHF-derived cells contribute to ascending aortopathies.

METHODS: Ascending aortic pathologies were examined in patients with sporadic thoracic aortopathies and angiotensin II (AngII)–infused mice. Ascending aortas without overt pathology from AngII-infused mice were subjected …

Development And Utilization Of An Isolation Box Test To Characterize Personality Traits Of Dairy Calves, Megan Marie Woodrum Setser, Heather W. Neave, Eric S. Vanzant, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

The use of an isolation box test (IBT) to characterize personality traits has been used in non-bovine species with success. We aimed to develop an IBT for dairy calves and determine if the behavioral responses to an IBT were associated with personality traits found from traditional tests (novel person, novel object, and a startle tests) and average daily gain (ADG; Kg/d) through weaning. Calf movement while in the IBT was measured via accelerometers attached to 5 locations on the exterior of the box. A total movement index (TMI) was calculated based on accelerometer readings during the IBT. We performed a …

Hpg80 (Circulating Progastrin), A Novel Blood-Based Biomarker For Detection Of Poorly Differentiated Neuroendocrine Carcinoma And Well Differentiated Neuroendocrine Tumors, Aman Chauhan, Alexandre Prieur, Jill Kolesar, Susanne Arnold, Léa Payen, Younes Mahi, Berengere Vire, Madison Sands, B. Mark Evers, Dominique Joubert, Lowell Anthony

Markey Cancer Center Faculty Publications

Current blood-based biomarkers for neuroendocrine neoplasms (NENs) lack both sensitivity and specificity. Human circulating progastrin (hPG₈₀) is a novel biomarker that can be easily measured in plasma by ELISA. This study is the first to examine hPG₈₀ in NENs. Plasma hPG₈₀ was quantified from 95 stage IV NEN patients, using DxPG₈₀ technology (ECS Progastrin, Switzerland) and compared with hPG₈₀ concentrations in two cohorts of healthy donor controls aged 50–80 (n = 252) and 18–25 (n = 137). Median hPG₈₀ in NENs patients was 5.54 pM compared to 1.5 pM for the …

Comparative Soil Organic Carbon Dynamics In Tropical And Subtropical Grassland Ecosystems, N. S. Pasricha, P. K. Ghosh

IGC Proceedings (1977-2023)

Grassland ecosystems play significant role in mitigating the climate change by sequestering atmospheric CO₂. One fifth of the total terrestrial C is stored in the root zone of grasslands as soil organic carbon. However, because of lack of proper management, overgrazing, and conversion to crop lands, these grasslands are becoming a source of CO₂ emissions. It has been observed that in Imperata grasslands of Northeast India, a third of total C captured annually is lost though CO₂ emissions. In the absence of intensified grazing and burning, these grasslands exhibit significantly high capacity to store SOC stocks. …

Climate Change Threats To Semi-Arid Transhumance Grazing Systems: Proposals For Adaptation In Cabo Verde, J. F. Castro, S. Tavares, M. Castro

IGC Proceedings (1977-2023)

Global warming has changed the rainfall regime in the Sahelian region, endangering and widespread poverty and chronic famines in Cape Verde, where rural communities' well-being and food security depend on rainy agriculture. Local responses to rainfall new patterns and scarcity have been livestock reduction with significant revenue losses. Together with dry mist occurrences, these environmental constraints demand to define priority actions to adapt and mitigate climate change's direct impacts. According to fifty household inquiries, this work explains the transhumant grazing practices on a semi-arid plateau in Tarrafal (Santiago, Cabo Verde), prospecting adaptation alternatives for sustainability. We related grazing patterns and …

Wild Red Deer Benefit The Conservation Of European Semi-Natural Open Habitats, F. Riesch, Bettina Tonn, Marcus Meißner, Sven Herzog, Niko Balkenhol, J. Isselstein

IGC Proceedings (1977-2023)

Traditional land use practices have shaped European landscapes for millennia. Agricultural intensification and declining popularity of pastoral farming in the past century have resulted in a tremendous loss of extensively used open landscapes and associated biodiversity. Today, conservation management needs to prevent secondary succession of many open habitats. Large or inaccessible target areas unsuitable for conventional conservation measures might benefit from grazing by wild herbivores, which do not require fencing nor regular welfare monitoring. In a military training area in Germany, we studied the quantitative and qualitative effects of wild red deer in two protected open habitat types (lowland hay …

Parasite Dynamics In Untreated Horses Through One Calendar Year, Ashley E. Steuer, Haley P. Anderson, Taylor Shepherd, Morgan Clark, Jessica A. Scare, Holli S. Gravatte, Martin K. Nielsen

Veterinary Science Faculty Publications

BACKGROUND: Horses are host to a plethora of parasites. Knowledge of the seasonality of parasite egg shedding and transmission is important for constructing parasite control programs. However, studies describing these patterns are sparse, and have largely been conducted only in the United Kingdom. This study evaluated strongylid egg shedding patterns and transmission dynamics of Strongylus vulgaris in naturally infected and untreated mares and foals through one calendar year in Kentucky, USA. The study also investigated the existence of a peri-parturient rise (PPR) in strongylid egg counts in foaling mares and collected information about Strongyloides westeri and Parascaris spp. in the …

Prostacyclin Promotes Degenerative Pathology In A Model Of Alzheimer’S Disease, Tasha R. Womack, Craig T. Vollert, Odochi Ohia-Nwoko, Monika Schmitt, Saghi Montazari, Tina L. Beckett, David Mayerich, M. Paul Murphy, Jason L. Eriksen

Molecular and Cellular Biochemistry Faculty Publications

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that is the most common form of dementia in aged populations. A substantial amount of data demonstrates that chronic neuroinflammation can accelerate neurodegenerative pathologies. In AD, chronic neuroinflammation results in the upregulation of cyclooxygenase and increased production of prostaglandin H2, a precursor for many vasoactive prostanoids. While it is well-established that many prostaglandins can modulate the progression of neurodegenerative disorders, the role of prostacyclin (PGI2) in the brain is poorly understood. We have conducted studies to assess the effect of elevated prostacyclin biosynthesis in a mouse model of AD. Upregulated prostacyclin expression …

Feasibility Of Using Ring-Mounted Halcyon Linac For Single-Isocenter/Two-Lesion Lung Stereotactic Body Radiation Therapy, Damodar Pokhrel, Aaron Webster, Richard Mallory, Justin Visak, Mark E. Bernard, Ronald C. Mcgarry, Mahesh Kudrimoti

Radiation Medicine Faculty Publications

PURPOSE: To demonstrate the plan quality and delivery efficiency of volumetric-modulated arc therapy (VMAT) with the Halcyon Linac ring delivery system (RDS) in the treatment of single-isocenter/two-lesion lung stereotactic body radiation therapy (SBRT).

MATERIALS/METHODS: Sixteen previously treated non-coplanar VMAT single-isocenter/two-lesion lung SBRT plans delivered with SBRT-dedicated C-arm TrueBeam Linac were selected. Prescribed dose was 50 Gy to each lesion over five fractions with treatment delivery every other day and AcurosXB algorithm as the final dose calculation algorithm. TrueBeam single-isocenter plans were reoptimized for Halcyon Linac with coplanar geometry. Both TrueBeam and Halcyon plans were normalized for identical combined target coverage …

Soil Health – It's Not All Biology, Mark S. Coyne, E. M. Pena-Yewtukhiw, John H. Grove, A. C. Sant'anna, D. Mata-Padrino

Plant and Soil Sciences Faculty Publications

Soil Health research tends to bias to a biology/microbiology emphasis. We believe this bias neglects important physical and chemical interactions in soil that are crucial to soil function. We offer several examples illustrating this bias, and how it may misrepresent management practices that have the greatest influence on Soil Health. Four suggestions are given as approaches to mitigate this bias. By appreciating soil structure as a foundation for Soil Health and its microbial community, we believe better recommendations can be made to assist the farm community in its stewardship of soil as a critical natural resource.

Kentucky’S Comprehensive Outreach Program For Horse Pasture Management And Education, Krista Lea, S. Ray Smith

IGC Proceedings (1977-2023)

The University of Kentucky (UK) Horse Pasture Evaluation Program began in 2005 to improve pasture quality and quantity on Kentucky horse farms. This on-farm program collects detailed data of pasture species composition, tests for tall fescue (Schedonorus arundinaceus (Schreb.) Dumort.) toxicity, and provides farm managers with comprehensive recommendations for management. Species composition is determined using the occupancy method. In 2019, the UK Horse Pasture Health Score Card was developed to provide useful, survey-level information on pastures and allow evaluators to cover far more acres in less time, requiring fewer resources. The objective of this research was to compare accuracy …

Improving Pasture Productivity And Persistence By Renovating Or Rejuvenating, A. Omokanye, D. Westerlund, G. Hernandez-Ramirez

IGC Proceedings (1977-2023)

Forages make up a large part of the feed requirements of beef cattle, and grazing remains the most economical form of feed delivery. However, several years after pasture establishment, forage productivity and longevity usually decline. Maintaining productive forage stands in the long term becomes a major challenge that beef producers face. A 3-yr on-farm study was conducted to determine the comparative effects of several pasture rejuvenation methods, such as spraying of herbicides to control weeds and brush, Spray herbicide + direct seed in spring (RSS), forage seeding methods, fertilizer application (FERT), and pasture rest as well as aeration/spiking in fall …

Cognitive Reserve And Mild Cognitive Impairment, Maryam Iraniparast, Yidan Shi, Ying Wu, Leilei Zeng, Colleen J. Maxwell, Richard J. Kryscio, Philip D. St. John, Karen S. Santacruz, Suzanne L. Tyas

Sanders-Brown Center on Aging Faculty Publications

Background and Objectives Little is known about the effect of education or other indicators of cognitive reserve on the rate of reversion from mild cognitive impairment (MCI) to normal cognition (NC) or the relative rate (RR) of reversion from MCI to NC vs progression from MCI to dementia. Our objectives were to (1) estimate transition rates from MCI to NC and dementia and (2) determine the effect of age, APOE, and indicators of cognitive reserve on the RR of reversion vs progression using multistate Markov modeling.

Methods We estimated instantaneous transition rates between NC, MCI, and dementia after accounting …

Dairy Cattle Genetics By Environment Interaction Mismatch Contributes To Poor Mitigation And Adaptation Of Grazing Systems To Climate Change Actions In The Peruvian High Andes: A Review, V. M. Velez Marroquin, J. Manhire, R. Garcia Ticllacuri, J. L. Bernal Madrid, C. S. Pinares-Patino

IGC Proceedings (1977-2023)

The high Andes of Peru includes fragile ecosystems. Nevertheless, it plays important ecosystem functions (e.g., biodiversity, water supply for the lowlands, CO₂ sinks in soil, etc). More than 80% of the livestock population of Peru is farmed in this area, supporting the livelihood of approximately 1’400,000 poor families, who are vulnerable to climate change (CC). Climate change in the high Andes is occurring at accelerated rates, compared to lowlands regions. Prevalent factors in the high Andes, such as hypoxia, high UV radiation, climatic extremes, large variation between maximum and minimum temperatures, seasonality in rainfall (determining highly seasonal forage growth) …

Exploring The Variation In Spatial Landscape Utilisation By Cows In The Pre- And Post-Partum Period Using Gnss Technology, A Z. Chang, M. G. Trotter

IGC Proceedings (1977-2023)

On-animal sensors refer to systems that monitor the location, behaviour, or physiological characteristics of livestock. These technologies provide a method to overcome many of the challenges that are associated with monitoring livestock within an extensive grazing system. A global navigation satellite system (GNSS) could be used to detect the changes in spatial utilisation associated with when a cow has a calf at foot. In this study, forty pregnant Belmont Red heifers were fitted with GNSS collars programmed to provide a location reading at 5 minute intervals. The data was cleaned and mapped in ArcGIS to identify changes in paddock area …

Sustainable Intensification Of Livestock Systems Using Forage Legumes, José C. B. Dubeux Jr., Lynn E. Sollenberger

IGC Proceedings (1977-2023)

Global human population is increasing and expected to reach 9.7 billion people by 2050. Sustainable intensification (SI) of agricultural systems is key to increase food production while minimizing impact on global natural resources. Forage legumes provide a myriad of ecosystem services (ES) and represent an important tool for promoting SI in livestock systems. Forage legumes associate with soil microorganisms to reduce atmospheric N₂. This N input represents a valuable contribution to increase net primary productivity with reduced C footprint. In addition, forage nutritive value generally increases, resulting in greater animal performance. When forage legumes are integrated into livestock …

The Optimum Grazing Time Based On Milk Fatty Acids And Yield For Holstein Lactating Cows Through Eco-Pastoral System In Alpine Grassland, M. Kim, J. Y. Kim, M. H. Jo, C. H. Kim, K. Sung

IGC Proceedings (1977-2023)

This study aimed to detect the optimum grazing time based on milk fatty acids and yield for the Holstein cow (Bos taurus) through eco-pastoral system in Korean alpine grassland. The treatment of grazing time was 3 levels: Non-grazing, Half-day grazing, Full-day grazing. First, the difference of milk fatty acids (saturated fatty acid, unsaturated fatty acid, Total ω-3, etc.) and milk yield was checked by grazing time via ANOVA. Second, various measurements of milk fatty acids were reduced to principal components for effective and obvious detecting the variation as a grazing time. Final, the variations of milk fatty acid …

Ppld Is A De-N-Acetylase Of The Cell Wall Linkage Unit Of Streptococcal Rhamnopolysaccharides, Jeffrey S. Rush, Prakash Parajuli, Alessandro Ruda, Jian Li, Amol Arunrao Pohane, Svetlana Zamakhaeva, Mohammad M. Rahman, Jennifer C. Chang, Artemis Gogos, Cameron W. Kenner, Gérard Lambeau, Michael J. Federle, Konstantin V. Korotkov, Göran Widmalm, Natalia Korotkova

Molecular and Cellular Biochemistry Faculty Publications

The cell wall of the human bacterial pathogen Group A Streptococcus (GAS) consists of peptidoglycan decorated with the Lancefield group A carbohydrate (GAC). GAC is a promising target for the development of GAS vaccines. In this study, employing chemical, compositional, and NMR methods, we show that GAC is attached to peptidoglycan via glucosamine 1-phosphate. This structural feature makes the GAC-peptidoglycan linkage highly sensitive to cleavage by nitrous acid and resistant to mild acid conditions. Using this characteristic of the GAS cell wall, we identify PplD as a protein required for deacetylation of linkage N-acetylglucosamine (GlcNAc). X-ray structural analysis indicates …

Forage News [2022-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

Prostacyclin Promotes Degenerative Pathology In A Model Of Alzheimer’S Disease, Tasha R. Womack, Craig T. Vollert, Odochi Ohia-Nwoko, Monika Schmitt, Saghi Montazari, Tina L. Beckett, David Mayerich, Michael Paul Murphy, Jason L. Eriksen

Sanders-Brown Center on Aging Faculty Publications

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that is the most common form of dementia in aged populations. A substantial amount of data demonstrates that chronic neuroinflammation can accelerate neurodegenerative pathologies. In AD, chronic neuroinflammation results in the upregulation of cyclooxygenase and increased production of prostaglandin H2, a precursor for many vasoactive prostanoids. While it is well-established that many prostaglandins can modulate the progression of neurodegenerative disorders, the role of prostacyclin (PGI2) in the brain is poorly understood. We have conducted studies to assess the effect of elevated prostacyclin biosynthesis in a mouse model of AD. Upregulated prostacyclin expression …

Kentucky Annual Economic Report 2022, Michael W. Clark, James P. Ziliak, Simon Sheather

Kentucky Annual Economic Report

This report is one of the important ways that the Center for Business and Economic Research fulfills its mission to examine various aspects of Kentucky’s economy as directed by the Kentucky Revised Statutes (KRS 164.738). The analysis and data presented here cover a variety of topics that range from a discussion of Kentucky’s current economic climate to a broad presentation of factors affecting the economy.

The report covers numerous dimensions of Kentucky’s economy including the effects of COVID-19. As the pandemic approaches its third year, COVID-19 continues to dominate the economic narrative. Many aspects of the economy have improved substantially …

Regulatory T Cells Control Effector T Cell Inflammation In Human Prediabetes, Rui Liu, Gabriella H. Pugh, Erin Tevonian, Katherine Thompson, Douglas A. Lauffenburger, Philip A. Kern, Barbara S. Nikolajczyk

Clinical and Translational Science Faculty Publications

A disparate array of plasma/serum markers provides evidence for chronic inflammation in human prediabetes, a condition that is most closely replicated by standard mouse models of obesity and metaflammation. These remain largely nonactionable and contrast with our rich understanding of inflammation in human type 2 diabetes. New data show that inflammatory profiles produced by CD4+ T cells define human prediabetes as a unique inflammatory state. Regulatory T cells (Treg) control mitochondrial function and cytokine production by CD4+ effector T cells (Teff) in prediabetes and type 2 diabetes by supporting T helper (Th)17 or Th1 cytokine production, respectively. These data suggest …

Serious Mental Illness And Mental Health Treatment Utilization Among Adults Residing In Non-Metropolitan And Metropolitan Counties, Tyrone F. Borders, Timothy Williams

Rural & Underserved Health Research Center Publications

Key Findings

The past year prevalence of serious mental illness (SMI) was significantly higher among non-metropolitan than metropolitan (5.90% vs. 5.18%, P < .03) adults.

Only 67.58% of non-metropolitan and 64.29% of metropolitan adults with SMI received any mental health (MH) treatment in the past year.

Additional analyses revealed the following non-metropolitan/metropolitan treatment differences:

• A higher percentage of non-metropolitan than metropolitan adults with SMI received only medication for MH treatment (24.50% vs. 18.53%, P < .02).
• A higher percentage of metropolitan than non-metropolitan adults with SMI received inpatient, outpatient, and medication (5.42% vs. 2.63%, P < .02).
• A significantly higher percentage of non-metropolitan than metropolitan adults with …

Volumetric Lattice Boltzmann Method For Wall Stresses Of Image-Based Pulsatile Flows, Xiaoyu Zhang, Joan Gomez-Paz, Xi Chen, James M. Mcdonough, Md Mahfuzul Islam, Yiannis Andreopoulos, Luoding Zhu, Huidan Yu

Mechanical Engineering Faculty Publications

Image-based computational fluid dynamics (CFD) has become a new capability for determining wall stresses of pulsatile flows. However, a computational platform that directly connects image information to pulsatile wall stresses is lacking. Prevailing methods rely on manual crafting of a hodgepodge of multidisciplinary software packages, which is usually laborious and error-prone. We present a new computational platform, to compute wall stresses in image-based pulsatile flows using the volumetric lattice Boltzmann method (VLBM). The novelty includes: (1) a unique image processing to extract flow domain and local wall normality, (2) a seamless connection between image extraction and VLBM, (3) an en-route …

Frequency And Types Of Healthcare Encounters In The Week Preceding A Sepsis Hospitalization: A Systematic Review, Alexander H. Flannery, Chad M. Venn, Amanda Gusovsky, Stephanie Henderson, Adam S. Kiser, Hallie C. Prescott, Chanu Rhee, Chris Delcher, Peter E. Morris

Pharmacy Practice and Science Faculty Publications

OBJECTIVES: Early recognition and treatment are critical to improving sepsis outcomes. We sought to identify the frequency and types of encounters that patients have with the healthcare system in the week prior to a sepsis hospitalization.

DATA SOURCES: PubMed, Cumulative Index to Nursing and Allied Health Literature, Scopus, and the Cochrane Library.

STUDY SELECTION: Observational cohort studies of patients hospitalized with sepsis or septic shock that were assessed for an outpatient or emergency department encounter with the healthcare system in the week prior to hospital admission.

DATA EXTRACTION: The primary outcome was the proportion of patients with a healthcare encounter …

Β-Aminopropionitrile-Induced Aortic Aneurysm And Dissection In Mice, Hisashi Sawada, Zachary A. Beckner, Sohei Ito, Alan Daugherty, Hong S. Lu

Saha Cardiovascular Research Center Faculty Publications

The mechanistic basis for the formation of aortic aneurysms and dissection needs to be elucidated to facilitate the development of effective medications. β-Aminopropionitrile administration in mice has been used frequently to study the pathologic features and mechanisms of aortic aneurysm and dissection. This mouse model mimics several facets of the pathology of human aortic aneurysms and dissection, although many variables exist in the experimental design and protocols that must be resolved to determine its application to the human disease. In the present brief review, we have introduced the development of this mouse model and provided insights into understanding its pathologic …

Using Culturally Focused Storytelling To Empower Appalachian Kentucky Youth To Understand And Address Cancer Disparities In Their Communities, Lauren K. Collett, Lauren Hudson, Chris Prichard, Nathan L. Vanderford

Markey Cancer Center Faculty Publications

Kentucky has the highest cancer incidence and mortality rates in the nation with the Appalachian region of the state being most affected. These rates are driven by health behaviors and inequities in social determinants of health. Herein, Appalachian Kentucky students with the University of Kentucky’s Appalachian Career Training in Oncology (ACTION) program were engaged in a storytelling exercise by writing culturally framed essays. Students discussed their personal experiences with cancer and their thoughts on the causes of and potential solutions to Appalachian Kentucky’s cancer disparities. Content analysis was used to identify common themes, subthemes, and subtopics in the essays regarding …

Avoidable And Unavoidable Repeat Breast Core Needle Biopsies, Xiaoqin Wang, Fara Shikoh, Mauro Hanaoka, Aurela Clark

Radiology Faculty Publications

Background: Breast core needle biopsies are not perfect and could miss cancer. The need for a repeat breast core biopsy is not uncommon and can occur for a multitude of reasons. Radiologists should carefully correlate the pathology results with imaging features after each breast biopsy and must recognize why certain core biopsies must be repeated to avoid missed or delayed cancer diagnosis. In this review, we discuss the main reasons for repeat core biopsies via case presentation with radiological images and pathological correlation. This review will help multidisciplinary breast care team recognize when to repeat a biopsy to reduce false …

Characteristics And Assessing Biological Risks Of Airborne Bacteria In Waste Sorting Plant, Abbas Norouzian Baghani, Somayeh Golbaz, Gholamreza Ebrahimzadeh, Marcelo I. Guzman, Mahdieh Delikhoon, Mehdi Jamshidi Rastani, Abdullah Barkhordari, Ramin Nabizadeh

Chemistry Faculty Publications

Examining the concentration and types of airborne bacteria in waste paper and cardboard sorting plants (WPCSP) is an urgent matter to inform policy makers about the health impacts on exposed workers. Herein, we collected 20 samples at 9 points of a WPCSP every 6 winter days, and found that the most abundant airborne bacteria were positively and negatively correlated to relative humidity and temperature, respectively. The most abundant airborne bacteria (in units of CFU m⁻³) were: Staphylococcus sp. (72.4) > Micrococcus sp. (52.2) > Bacillus sp. (30.3) > Enterococcus sp. (24.0) > Serratia marcescens (20.1) > E. coli (19.1) > Pseudomonas sp. (16.0) > Nocardia …

Perceptions On Governance For Effective Adaptation To Climate Change Within Community-Based Wildlife Conservancies In Kenya, S. Kibet, Oliver Vivian Wasonga

IGC Proceedings (1977-2023)

Community-based wildlife conservancies (CBC) represent a broad spectrum of new management arrangements and benefit sharing partnerships in natural resource management by none state agents, but who, by virtue of their collective location and activities, are critically placed to shape the present and future status of these resources. This approach of wildlife management is increasingly gaining popularity as an option for achieving sustainable co-existence and complementarity between wildlife conservation and livestock production in the drylands of Kenya. Despite difference in ownership and governance structure in place, all conservancies have instituted new rules of control and access. This study sought to understand …