Digital Commons Network^™

Neuronal Cell Model And Methods Of Use Therefor, Craig S. Miller, Robert J. Danaher, Robert J. Jacob

Microbiology, Immunology and Molecular Genetics Faculty Patents

The present invention provides, inter alia, a PC12 cell quiescently infected with a neurotropic virus. Preferred quiescently-infected neural cells are those wherein the neurotropic virus is a neurotropic herpes virus. Methods related to these and other materials are also provided.

Diagnostic Test And Therapy For Manganese Superoxide Dismutate (Msod) Associated Diseases, Daret K. St. Clair, Muneyasu Urano, Edward J. Kasarskis

Toxicology and Cancer Biology Faculty Patents

The present invention provides a diagnostic method and a kit for detection of mutations localized within the 5′ promoter region of the MnSOD gene. Such mutations are associated with diseases characterized by decreased MnSOD activity such as certain formes of cancer, and ALS. Accordingly, the diagnostic method this invention provides, comprising RFLP, direct sequencing, or PCR analysis of the region within 3 kb, the transcription initiation site will detect these disorders. This invention also provides a therapeutic method for such disorders comprising transfection of affected cells or tissues with high activity, MnSOD expression vectors, or the administration of exogenous MnSOD …

The Toxoplasma Gondii Protein Rop2 Mediates Host Organelle Association With The Parasitophorous Vacuole Membrane, Anthony P. Sinai, Keith A. Joiner

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii replicates within a specialized vacuole surrounded by the parasitophorous vacuole membrane (PVM). The PVM forms intimate interactions with host mitochondria and endoplasmic reticulum (ER) in a process termed PVM–organelle association. In this study we identify a likely mediator of this process, the parasite protein ROP2. ROP2, which is localized to the PVM, is secreted from anterior organelles termed rhoptries during parasite invasion into host cells. The NH₂-terminal domain of ROP2 (ROP2hc) within the PVM is exposed to the host cell cytosol, and has characteristics of a mitochondrial targeting signal. In in vitro assays, ROP2hc is …

Protection Against Traumatic Brain Injury, Stephen William Scheff

Sanders-Brown Center on Aging Faculty Patents

The present invention relates to therapeutic uses of cyclosporin A to reduce adverse effects of neural injury.

28th Annual Midwest/Midsouth Estate Planning Institute, Office Of Continuing Legal Education At The University Of Kentucky College Of Law

Continuing Legal Education Materials

Materials from the 28th Annual Midwest/Midsouth Estate Planning Institute held by UK/CLE in July 2001.

Estate Litigation In Kentucky, Office Of Continuing Legal Education At The University Of Kentucky College Of Law

Continuing Legal Education Materials

Materials from the conference on Estate Litigation in Kentucky held by UK/CLE in July 2001.

Forage News [2001-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Robinson Station Celebrates 75^TH Anniversary
• Economic Benefits of Stockpiled Fescue on Two Northeast Arkansas Farms
• Performance of Cattle on “HIMAG” Tall Fescue Infected with Novel Endophytes
• Determination of Pregnancy Outcome of Mares Grazed on a Toxic-Free Endophyte-Infected Tall Fescue (MaxQ)
• Cutting Height to Maximize Yield and Quality
• Sampling Medium Rectangular Bales: How Many Cores are Enough?
• Upcoming Events

Raising The Social Security Retirement Ages: Weighing The Costs And Benefits, Kathryn L. Moore

Law Faculty Scholarly Articles

The Social Security program faces a long-term funding deficit. The Board of Trustees of the Federal Old-Age and Survivors and Disability Insurance ("OASDI") Trust Funds predicts that unless corrective action is taken, Social Security benefit payments will exceed dedicated tax revenues by the year 2015, and the Social Security program will become insolvent—unable to pay promised benefits in full-by the year 2037. As a result of this projected deficit, Social Security has become "a lightning rod for far reaching reform proposals."

Proposals range from "traditional" proposals that would maintain the basics of the program's revenue and benefit structure but would …

The Kentucky Plan: An Innovative Approach To Professional Doctoral Education In Public Health., Joel M. Lee, F. Douglas Scutchfield

Preventive Medicine and Environmental Health Faculty Publications

The Doctor of Public Health (Dr.P.H.) degree is designed to prepare graduates for the highest levels of leadership in public health related careers in both public and private settings. The Kentucky School of Public Health has developed an innovative generalist professional degree program offering an extensive battery of courses based on the body of professional public health knowledge. The degree includes extensive supervised field experience as part of the academic training, linking theory with practice. Issues related to professional degrees, curriculum, accreditation and certification, admission, evaluation, plans for institutional cooperation, and demand are addressed.

State Road Fund Revenue Collection Processes: Differences And Opportunities Of Improved Efficiency, Robert J. Eger Iii, Merl M. Hackbart

Kentucky Transportation Center Research Report

Research regarding the administration and collection of road fund revenues has focused on gaining an understanding of the motivations for tax evasion, methods of evasion, and estimates of the magnitude of evasion for individual states. To our knowledge, little attention has been focused on the impact road fund collection, assessment, audit, and enforcement processes have on tax evasion. The purpose of this study is to review the current road fund assessment, collection, audit, and enforcement processes and procedures and to develop recommendations to improve the efficiency and effectiveness of the process.

Attitudes Towards Guns: Associations With Alcohol Use And Impulsive Behaviors, Catherine A. Martin, Arch G. Mainous, Harriett H. Ford, Rosalie Mainous, Susan Slade, David Martin, Hatim A. Omar

Psychiatry Faculty Publications

This study was an investigation of the association of attitudes toward guns with self-reports of alcohol/drug use, and impulsivity. Participants included 160 male and female high school students, who completed five questions regarding attitudes toward guns, in addition to questions about alcohol/drug use. Data were analyzed using t-tests. Males were more likely to feel that a home was safer with a gun. Feeling positively about a gun was associated with alcohol use in males and impulsive, aggressive behavior in males and females. A greater understanding of attitudes toward guns must take into account gender, alcohol use, and impulsive and aggressive …

Tetrafluoroazidoaniline And Method Of Making And Using The Same, Hans Peter Spielmann, Kareem A.H. Chehade

Molecular and Cellular Biochemistry Faculty Patents

The invention is directed to a novel compounds comprising 4-azidotetrafluoroaniline and the alkyl, acyl and sulfonamide derivatives thereof and to methods of making and using the same. The novel compounds are useful as a photoaffinity probe to study protein structure and function. Two methods for preparing 4-azidotetrafluoroaniline are disclosed, each employing a stable carbamate intermediate from which the 4-azidotetrafluoroaniline is derived.

Adolescent And Young Adult Substance Use: Association With Sensation Seeking, Self Esteem And Retrospective Report Of Early Pubertal Onset. A Preliminary Examination, Catherine A. Martin, T. K. Logan, Carl Leukefeld, Rich Milich, Hatim Omar, Richard Clayton

Psychology Faculty Publications

Structured questionnaires were administered to investigate the relationship between early pubertal onset, substance abuse, sensation seeking, and self-esteem. The current study presents data from 1,002 subjects, who were followed from the 6th to the 10th grades and again at the age of 20. In females, early pubertal onset was associated with greater cigarette use and lower self-esteem. Further the interaction of early pubertal onset and low self-esteem was predictive for cigarette use in females, as was early pubertal onset and high sensation seeking. Late pubertal onset was associated with decreased alcohol use and lower sensation seeking in males, with the …

The Impact Of A New Bypass Route On The Local Economy And Quality Of Life, Eric C. Thompson, Joseph Miller, Jonathon Roenker

Kentucky Transportation Center Research Report

Highway improvements such as bypass construction typically are motivated by a desire to improve the flow and safety of travel. But, given the importance of travel, transportation improvement projects often can affect the local economy and quality of life. This study assesses these potential impacts. The study was conducted by the University of Kentucky Center for Business and Economic Research, as part of a subcontract with the Kentucky Transportation Center. The study is one component of a larger Kentucky Transportation Center study entitled Planning Decisions Related to Bypass Construction, Life-Cycle Costs of Access Control, and Safety Impacts of Rural Road …

Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto

Veterinary Science Faculty Publications

In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I₅₀ of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according …

Pride Water Quality Assessment Report: Iv. Nutrient Assessment, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Research Reports

The efficient utilization of federal funds in improving the water quality and aquatic habitat of the region requires a mechanism for assessing and evaluating the impacts of the proposed and ongoing projects as well as some mechanism for prioritizing the allocation of additional funds. In order to evaluate the effectiveness of these projects it is important to provide a formal monitoring and assessment program based on sound scientific principles. This report provides an initial 10 year baseline assessment of the existing water quality conditions in the 40 county PRIDE region for the purpose of evaluating the impacts of the PRIDE …

Use Of The Naturally-Occuring Quinones Thymoquinone And Dithymoquinone As Antineoplastic And Cytotoxic Agents, Peter A. Crooks, David R. Worthen, Omar A. Ghosheh

Pharmaceutical Sciences Faculty Patents

Nigella sativa derivatives, thymoquinone (TM) and dithymoquinone (DIM) are used in treatment of parental and multi-drug resistant human cancers.

The Many Inventions Of Childhood: A Tentative Taxonomy, Leonora Ritter

disClosure: A Journal of Social Theory

No abstract provided.

Squirrel Nutkin, Faye Moskowitz

disClosure: A Journal of Social Theory

No abstract provided.

Childhood And Child Life. Disclosure Interviews Jo Boyden, Margo Kleinfeld

disClosure: A Journal of Social Theory

No abstract provided.

Cyber Children: Discursive And Subjective Practices In The Palace, Angela Thomas

disClosure: A Journal of Social Theory

No abstract provided.

Seaall Chapter Handbook And Procedures Manual, April 2001, Seaall

Administrative Materials

This handbook is a revised and updated version of the March 2000 SEAALL Chapter Handbook and Procedures Manual.

Innovative Financing Options For Kentucky’S Transportation Infrastructure, Merl M. Hackbart

Kentucky Transportation Center Research Report

A strong linkage exists between economic growth and the availability of an adequate and efficient transportation system. Due to its location, geography and economy, this linkage — particularly in regard to highway transportation — is critical to continued economic progress in the Commonwealth of Kentucky. However, Kentucky’s ability to provide the infrastructure investments needed to be competitive in the new “global economy” is constrained by the structure of its funding mechanism, the Road Fund. During the last two decades, Road Fund revenue growth has dramatically lagged the growth of motor vehicle travel. As a consequence, Kentucky faces highway investment needs …

Aluminium Toxicokinetics: An Updated Minireview, Robert A. Yokel, Patrick J. Mcnamara

Pharmaceutical Sciences Faculty Publications

This MiniReview updates and expands the MiniReview of aluminium toxicokinetics by Wilhelm et al. published by this journal in 1990. The use of ²⁶Al, analyzed by accelerator mass spectrometry, now enables determination of Al toxicokinetics under physiological conditions. There is concern about aluminium in drinking water. The common sources of aluminium for man are reviewed. Oral Al bioavailability from water appears to be about 0.3%. Food is the primary common source. Al bioavailability from food has not been adequately determined. Industrial and medicinal exposure, and perhaps antiperspirant use, can significantly increase absorbed aluminium. Inhalation bioavailability of airborne soluble Al …

Estrogen Protects Against The Synergistic Toxicity By Hiv Proteins, Methamphetamine And Cocaine, Jadwiga Turchan, Caroline Anderson, Kurt F. Hauser, Qinmiao Sun, Jiayou Zhang, Ying Liu, Phyllis M. Wise, Inna Kruman, William Maragos, Mark P. Mattson, Rosemarie Booze, Avindra Nath

Neurology Faculty Publications

BACKGROUND: Human immunodeficiency virus (HIV) infection continues to increase at alarming rates in drug abusers, especially in women. Drugs of abuse can cause long-lasting damage to the brain and HIV infection frequently leads to a dementing illness. To determine how these drugs interact with HIV to cause CNS damage, we used an in vitro human neuronal culture characterized for the presence of dopaminergic receptors, transporters and estrogen receptors. We determined the combined effects of dopaminergic drugs, methamphetamine, or cocaine with neurotoxic HIV proteins, gp120 and Tat.

RESULTS: Acute exposure to these substances resulted in synergistic neurotoxic responses as measured by …

3rd Annual Computer & Technology Law Institute, Office Of Continuing Legal Education At The University Of Kentucky College Of Law

Continuing Legal Education Materials

Materials from the 3rd Annual Computer & Technology Law Institute held by UK/CLE in March 2001.

Forage News [2001-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Alfalfa Awards
• 2001 Kentucky Grazing School
• Kentucky Cattle Numbers Rise Slightly
• How Widespread is the Practice of Management Intensive Rotational (MIRG) Grazing in Wisconsin?
• Little Benefit from Sclerotinia-resistant Alfalfa Varieties
• 21^ST Kentucky Alfalfa Conference
• Upcoming Events

Value Of Research: Spr Projects From 1995 To 1999, Donald L. Hartman

Kentucky Transportation Center Research Report

This report provides summary documentation of the 45 “SPR” research projects funded at the Kentucky Transportation Center (University of Kentucky) from 1995 through 1999. Eleven of these projects were selected for highlighting with up-to-date information regarding results and benefits. They represent a diverse range of research and demonstrate considerable breadth in the implementation of results. Abstracts for all the projects are include in the appendix. A brief discussion of the need/purpose of research, the research process, and the program areas of research at the Kentucky Transportation Center is included.

Report On County Road Finance And Fhwa Biennial Form 1998-1999, Doug Kreis

Kentucky Transportation Center Research Report

This report shows the revenues and expenditures associated with county and city resources for fiscal year 1998-1999 within the state of Kentucky. Federal Highway Form 536, which is included as the primary product of this project, shows where all revenues were generated as well as all allocations of the local highway funds. Also included within this report is a detailed report on county road finance, which is widely used within government throughout Kentucky to determine where allocated funding has been spent.

Enhanced Expression Of Human Platelet-Derived Growth Factor In Pichia Pastoris, David M. Kaetzel

Pharmacology and Nutritional Sciences Faculty Patents

Enhanced yields of the platelet-derived growth factor (PDGF) B-chain are obtained using the Pichia pastoris yeast system. Yields of both wild-type and mutant human proteins are enhanced when the yeast is transformed with a vector containing the yeast mating factor promoter fused to a sequence encoding the mature protein. The secreted wild-type protein is indistinguishable from mature 29-32 kDa protein isolated from human and Chinese hamster ovary (CHO) cells. An RKK→EEE mutant exhibited reduced association with the cell surface and accumulated in the culture medium as 29-32 kDa forms. Stable transfection of U87 astrocytoma cells with RKK→EEE mutants of either …