Digital Commons Network^™

Reactive Oxygen Species Mediate Tnfr1 Increase After Trpv1 Activation In Mouse Drg Neurons, Fei Ma, Liping Zhang, Karin N. Westlund

Physiology Faculty Publications

Background: Transient receptor potential vanilloid subtype 1 (TRPV1) is activated by low pH/protons and is well known to be involved in hyperalgesia during inflammation. Tumor necrosis factor α (TNF-α), a proinflammatory cytokine, is involved in nociceptive responses causing hyperalgesia through TNF receptor type 1 (TNFR1) activation. Reactive oxygen species (ROS) production is also prominently increased in inflamed tissue. The present study investigated TNFR1 receptors in primary cultured mouse dorsal root ganglion (DRG) neurons after TRPV1 activation and the involvement of ROS. C57BL/6 mice, both TRPV1 knockout and wild type, were used for immunofluorescent and live cell imaging. The L4 and …

Opportunities For Comparative Research In Public Health Pbrns : A Baseline Analysis Of Local Practice Settings, Glen P. Mays, Sharla A. Smith, Elaine B. Wootten, Sylvia J. Porchia

Health Management and Policy Presentations

This analysis describes the organizational and operational characteristics of local public health agencies participating in an initial cohort of five (5) public health PBRNs in the U.S. We examine variation in practice settings within and between PBRNs; compare practice settings to state and national norms; and identify opportunities for comparative research that can be conducted through PBRNs.

Externalities In The Classroom: How Children Exposed To Domestic Violence Affect Everyone’S Kids, Scott E. Carrell, Mark L. Hoekstra

University of Kentucky Center for Poverty Research Discussion Paper Series

There is widespread perception that externalities from troubled children are significant, though measuring them is difficult due to data and methodological limitations. We estimate the negative spillovers caused by children from troubled families by exploiting a unique data set in which children’s school records are matched to domestic violence cases. We find that children from troubled families significantly decrease their peers’ reading and math test scores and increase misbehavior in the classroom. The achievement spillovers are robust to within-family differences and when controlling for school-by-year effects, providing strong evidence that neither selection nor common shocks are driving the results.

A Retrospective Claims Analysis Of Combination Therapy In The Treatment Of Adult Attention-Deficit/Hyperactivity Disorder (Adhd), Gerhardt M. Pohl, David L. Van Brunt, Wenyu Ye, William W. Stoops, Joseph A. Johnston

Behavioral Science Faculty Publications

BACKGROUND: Combination therapy in managing psychiatric disorders is not uncommon. While combination therapy has been documented for depression and schizophrenia, little is known about combination therapy practices in managing attention-deficit/hyperactivity disorder (ADHD). This study seeks to quantify the combination use of ADHD medications and to understand predictors of combination therapy.

METHODS: Prescription dispensing events were drawn from a U.S. national claims database including over 80 managed-care plans. Patients studied were age 18 or over with at least 1 medical claim with a diagnosis of ADHD (International Classification of Diseases, Ninth Revision, Clinical Modification [ICD-9-CM] code 314.0), a pharmacy claim for …

Phylogenetic Analyses Reveal Monophyletic Origin Of The Ergot Alkaloid Gene Dmaw In Fungi, Miao Liu, Daniel G. Panaccione, Christopher L. Schardl

Plant Pathology Faculty Publications

Ergot alkaloids are indole-derived mycotoxins that are important in agriculture and medicine. Ergot alkaloids are produced by a few representatives of two distantly related fungal lineages, the Clavicipitaceae and the Trichocomaceae. Comparison of the ergot alkaloid gene clusters from these two lineages revealed differences in the relative positions and orientations of several genes. The question arose: is ergot alkaloid biosynthetic capability from a common origin? We used a molecular phylogenetic approach to gain insights into the evolution of ergot alkaloid biosynthesis. The 4-γ,γ-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway. Amino acid sequences deduced …

Cancer Information-Seeking Experiences: The Implications Of Hispanic Ethnicity And Spanish Language, Robin C. Vanderpool, Julie Kornfeld, Lila Finney Rutten, Linda Squiers

Health, Behavior & Society Faculty Publications

BACKGROUND: Strategies to support cancer information-seeking among Hispanics are needed.

METHODS: We analyzed data from the 2005 Health Information National Trends Survey (HINTS) to explore cancer information-seeking experiences among respondents according to ethnicity and language of interview.

RESULTS: Over 80% of Spanish-speaking Hispanics had never looked for cancer information. Compared to English-speaking respondents, Spanish-speaking Hispanics who sought cancer information indicated their search took a lot of effort (67%), was hard to understand (54%), and frustrating (42%). Spanish-speaking Hispanics noted minimal confidence in obtaining cancer information.

CONCLUSIONS: Language and cultural differences must be considered in the design, implementation, and dissemination of …

Bridging The Gaps: Biomedicine, Complementary And Alternative Medicine In A Holistic Approach, Hatim A. Omar, Joav Merrick

Pediatrics Faculty Publications

The 4^th international conference on holistic health and medicine was held during September 24-26, 2008 in Lexington, Kentucky in the United States of America. This conference brought many participants from 23 countries, both mainstream health care providers as well as holistic health practitioners to address the latest in knowledge and research, and in turn to find ways to collaborate and work together to provide the best possible care for their patients and clients. These conferences are held to build bridges between the various fractions of medicine and health care providers. The scientific program included plenary sessions, keynotes, workshops and …

Weight Gain Associated With Hormonal Contraception Use In Adolescents Is Not Different From Control, Abdel Salous, Valerie Jones, Maria Melguizo Castro, Linah Al-Alem, Hatim A. Omar

Pediatrics Faculty Publications

The use of hormonal contraception is commonly associated with weight gain. The purpose of this retrospective study was to explore the association between the use of hormonal contraception and weight change in an outpatient adolescent patient population in Lexington, Kentucky. Data were gathered from the charts of 259 adolescent female patients seen in an outpatient adolescent clinic for up to 72 months. At the initial visit, all patients were evaluated for risk-taking behavior and received preventive counseling. Patients were categorized as either contraception users or non-users (control group). Contraception users were subdivided into Depot Medroxyprogesterone Acetate (DMPA) user group or …

It Is More Than Just A Reproductive Healthcare Visit: Experiences From An Adolescent Medicine Clinic, Marlene B. Huff, Kimberly K. Mcclanahan, Heather A. Brown, Hatim A. Omar

Pediatrics Faculty Publications

The gynecologist may be the only physician that an adolescent depends on for primary and reproductive healthcare services. Because adolescent females often make personal healthcare decisions without the benefit of supportive parents/caregivers, it is imperative that the gynecologist understands the developmental issues encountered during this phase of life. The objective of this study is to identify cited complaints of adolescent females seeking reproductive care and, thus, provide gynecologists with needed information about the medical/mental health issues that may be encountered during routine reproductive care exams. The study group was adolescent females between the ages of 12 and 20 years old. …

Holistic Approach To Health, Hatim A. Omar, Joav Merrick

Pediatrics Faculty Publications

No abstract provided.

Driver Of A Semi-Tractor Pulling Twin Trailers Fatally Injured In Single Vehicle Crash, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Motor Vehicle

In the spring of 2008, a 40-year-old tractor-trailer driver died after his vehicle left the interstate highway, crashed, and was engulfed in flames. The tractor drifted over the fog line and rumble strip onto the right shoulder, began to skid and left the highway. The tractor slid through the grass beside the roadway, struck a concrete culvert, sideswiped a small tree and then hit a large tree head-on. The tractor and trailers then skidded back down the embankment toward the highway where the entire unit came to rest. The tractor and lead trailer overturned onto the driver’s side. The second …

Information Remix: Meeting Service Challenges In A Self Service Environment, Antoinette Paris Greider

Library Presentations

No abstract provided.

Genes Encoding Several Poly (Adp-Ribose) Glycohydrolase (Parg) Enzymes, The Proteins And Fragments Thereof, And Antibodies Immunoreactive Therewith, Myron Jacobson, Elaine L. Jacobson, Jean-Christoph Amé, Winston Lin

Pharmaceutical Sciences Faculty Patents

The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods …

A Gene Expression Predictor Of Response To Egfr-Targeted Therapy Stratifies Progression-Free Survival To Cetuximab In Kras Wild-Type Metastatic Colorectal Cancer, Justin M. Balko, Esther P. Black

Pharmaceutical Sciences Faculty Publications

BACKGROUND: The anti-EGFR monoclonal antibody cetuximab is used in metastatic colorectal cancer (CRC), and predicting responsive patients garners great interest, due to the high cost of therapy. Mutations in the KRAS gene occur in ~40% of CRC and are a negative predictor of response to cetuximab. However, many KRAS-wildtype patients do not benefit from cetuximab. We previously published a gene expression predictor of sensitivity to erlotinib, an EGFR inhibitor. The purpose of this study was to determine if this predictor could identify KRAS-wildtype CRC patients who will benefit from cetuximab therapy.

METHODS: Microarray data from 80 metastatic CRC patients subsequently …

Coal Truck Driver Dies In Rollover After Tire On Trailer Fails, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Motor Vehicle

In the fall of 2006, a twenty-three year old male tractor-trailer driver who was hauling coal, died when a rear outer tire on the trailer failed. After the tire failed, the trailer slipped off the right side of the road, overturned, and went over an embankment. The trailer remained attached to the tractor as both went over the embankment, flipping the entire unit upside down. A witness called emergency medical services who arrived and called the county coroner. The coroner arrived and declared the driver dead at the scene. It was determined by Kentucky State Police that the driver was …

University Of Kentucky Adult Learning Focused Institution (Alfi) Task Force: Findings And Recommendations, Randolph Hollingsworth

Undergraduate Education White Papers

This report to Provost Kumble Subbaswamy details the wide-ranging work over two years of the UK Adult Learning Focused Institution (ALFI) Task Force. The findings in sum assert that the undergraduate population at the University is primarily of a traditional age and that little explicit attention is given to adult learners in UK's stated policies or procedures. The recommendations are that by becoming more "adult friendly" the University could also provide more flexibility and promise for success to all its students.

Striatal Neuroinflammation Promotes Parkinsonism In Rats, Dong-Young Choi, Mei Liu, Randy L. Hunter, Wayne A. Cass, Jignesh D. Pandya, Patrick G. Sullivan, Eun-Joo Shin, Hyoung-Chun Kim, Don M. Gash, Guoying Bing

Neuroscience Faculty Publications

BACKGROUND: Sporadic Parkinson's disease (PD) is a progressive neurodegenerative disorder with unknown cause, but it has been suggested that neuroinflammation may play a role in pathogenesis of the disease. Neuroinflammatory component in process of PD neurodegeneration was proposed by postmortem, epidemiological and animal model studies. However, it remains unclear how neuroinflammatory factors contribute to dopaminergic neuronal death in PD.

FINDINGS: In this study, we analyzed the relationship among inducible nitric oxide synthase (iNOS)-derived NO, mitochondrial dysfunction and dopaminergic neurodegeneration to examine the possibility that microglial neuroinflammation may induce dopaminergic neuronal loss in the substantia nigra. Unilateral injection of lipopolysaccharide (LPS) …

Chemical Weathering In A Dry Ash Dump: An Insight From Physicochemical And Mineralogical Analysis Of Drilled Cores, M W. Gitari, O Fatoba, A Nyamihingura, L F Petrik

World of Coal Ash Proceedings

No abstract provided.

Biochemical Response Of Landfill With Manufactured Aggregates As A Daily Cover, Zalleris Escobar, Yahaira Lugo, Sangchul Hwang

World of Coal Ash Proceedings

No abstract provided.

Environmental And Health Aspects Of Ashes Produced At Co-Combustion Of Biomass, Henk Te Winkel

World of Coal Ash Proceedings

No abstract provided.

Compressive Strength And Embodied Energy Optimization Of Fly Ash Based Geopolymer Cement Concrete, Brett Tempest, Olanrewaju Sanusi, Janos Gergely, Vincent Ogunro

World of Coal Ash Proceedings

No abstract provided.

Life Cycle Cost Minimization For Cement Production Under Various Constraints, Hakob Avetisyan

World of Coal Ash Proceedings

No abstract provided.

Case Study: Use Of Circulating Fluidized Bed Boiler Byproduct To Solidify Oily Sludge, J.F. Beech, Scott Elder

World of Coal Ash Proceedings

No abstract provided.

Practical Recommendations For The Use Of Pulverised Fuel Ash As An Engineering Fill In The Uk, Ian Fox, Bob Coombs

World of Coal Ash Proceedings

No abstract provided.

Coal Combustion By-Product Tools And Mine Pools: Environmental And Geotechnical Engineering Comments From Maryland, Paul A. Petzrick

World of Coal Ash Proceedings

No abstract provided.

Influence Of Industrial Solid Wastes On Soil-Plant Interactions In Rice Under Acid Lateritic Soil, S. Karmakar, B. N. Mittra, B. C. Ghosh

World of Coal Ash Proceedings

No abstract provided.

Potential Use Of Surfactant-Modified Zeolite For Attenuation Of Trace Elements In Fly Ash Leachate, Ghanashyam Neupane, Rona J. Donahoe

World of Coal Ash Proceedings

No abstract provided.

Soil Chemistry Still Affected 23 Years After Large Application Of Fluidized Bed Combustion Ash, Eton E. Codling, Akanksha W. Raja

World of Coal Ash Proceedings

No abstract provided.

Mercury Inhalation Risks In Indoor Air From Use Of Coal Combustion Products (Ccps) In Building Materials, Christopher M. Long, Ari S. Lewis, Sonja S. Sax

World of Coal Ash Proceedings

No abstract provided.

Mercury Inhalation Risks From Use Of Coal Combustion Products (Ccps) As Structural Fill And From Disposal Of Ccp-Containing Wallboard And Concrete In Landfills, Ari S. Lewis, Sonja S. Sax, Christopher M. Long

World of Coal Ash Proceedings

No abstract provided.