Digital Commons Network^™

Bridging The Care Continuum: Patient Information Needs For Specialist Referrals., Carol L. Ireson, Svetla Slavova, Carol L. Steltenkamp, F. Douglas Scutchfield

Preventive Medicine and Environmental Health Faculty Publications

BACKGROUND: Information transfer is critical in the primary care to specialist referral process and has been examined extensively in the US and other countries, yet there has been little attention to the patient's perspective of the information transfer process. This cross-sectional study examined the quality of the information received by patients with a chronic condition from the referring and specialist physician in the specialist referral process and the relationship of the quality of information received to trust in the physicians.

METHODS: Structured telephone interviews were conducted with a random sample of 250 patients who had experienced a referral to a …

Transcriptional Profiling Differences For Articular Cartilage And Repair Tissue In Equine Joint Surface Lesions, Michael J. Mienaltowski, Liping Huang, David D. Frisbie, C. Wayne Mcilwraith, Arnold J. Stromberg, Arne C. Bathke, James N. Macleod

Veterinary Science Faculty Publications

BACKGROUND: Full-thickness articular cartilage lesions that reach to the subchondral bone yet are restricted to the chondral compartment usually fill with a fibrocartilage-like repair tissue which is structurally and biomechanically compromised relative to normal articular cartilage. The objective of this study was to evaluate transcriptional differences between chondrocytes of normal articular cartilage and repair tissue cells four months post-microfracture.

METHODS: Bilateral one-cm2 full-thickness defects were made in the articular surface of both distal femurs of four adult horses followed by subchondral microfracture. Four months postoperatively, repair tissue from the lesion site and grossly normal articular cartilage from within the same …

Workers Electrocuted While Working In Elevated Buckets, Kentucky Injury Prevention And Research Center

Kentucky Haz Alerts--Motor Vehicle

To prevent worker electrocutions while working near over-head power lines:

• Employers should implement and enforce a formal written electrical safety training program that includes work around overhead lines.
• An observer should be designated by the employer to perform an electrical hazard
• evaluation prior to commencing new work activities.
• A hazard assessment of the job site and route of travel, including aerial and ground hazards and obstacles, within the job site should be performed by a competent person before work commences.

Distribution Of News Information Through Social Bookmarking: An Examination Of Shared Stories In The Delicious Website, Deborah S. Chung, Kwan Yi

Journalism and Media Faculty Publications

Introduction. This study examined the selection and sharing of news stories from Delicious, a popular social bookmarking site, in order to identify the most frequently consulted news information sources and news topics.

Method. Targeting US-specific sources through initial computer screening of URLs, we employed content analysis to further analyse story topics and sources that were unclassified through the initial computer screening method.

Analysis. After examining frequencies and percentages of the variables, a qualitative analysis was employed to assess relationships between story sources and story topics.

Results. Findings document the diverse nature of stories from both traditional and new media …

Economic Stress And Domestic Violence, Claire M. Renzetti

CRVAW Faculty Research Reports and Papers

The severe economic recession that began in December 2007 has renewed interest in the relationship between economic stress and domestic violence (DV). Although analyses of current financial statistics in relation to reported DV incidents have yet to be completed, a sizable body of research that examines various economic indicators provides a framework for understanding how economic stress may contribute to DV. At the same time, available research indicates that DV may also produce financial hardship for DV victims. This paper reviews the research on the reciprocal economic stressóDV relationship, focusing in particular on employment issues; social support networks; physical and …

Sexuality And Persons With Down Syndrome. A Study From Brazil, Bruna Marques Bononi, Maria José Carvalho Sant'anna, André Chao Vasconcellos De Oliveira, Tadeu Silveira Renattini, Carla Franchi Pinto, Maria Lúcia Passarelli, Verônica Coates, Hatim A. Omar

Pediatrics Faculty Publications

In recent years, important gains and changes have been observed in the life of teenagers with Down syndrome (DS) with increased inclusion into society. This review will discuss adolescence and sexuality in teenagers with DS from a descriptive study of 50 patients with DS between the ages of 10 and 20 years. The mean age was 13.5 years, 50% females; 86% went to school with 62.2% in school for over six years. Of the patients that attended school, 60% went to special education school and only 10% read and wrote correctly. In an evaluation of autonomy, 66% took showers, 78% …

Effects Of Genetic Deficiency Of Cyclooxygenase-1 Or Cyclooxygenase-2 On Functional And Histological Outcomes Following Traumatic Brain Injury In Mice, Matthew L. Kelso, Stephen W. Scheff, James R. Pauly, Charles D. Loftin

Pharmaceutical Sciences Faculty Publications

BACKGROUND: Neuroinflammation contributes to the pathophysiology of acute CNS injury, including traumatic brain injury (TBI). Although prostaglandin lipid mediators of inflammation contribute to a variety of inflammatory responses, their importance in neuroinflammation is not clear. There are conflicting reports as to the efficacy of inhibiting the enzymes required for prostaglandin formation, cyclooxygenase (COX) -1 and COX-2, for improving outcomes following TBI. The purpose of the current study was to determine the role of the COX isoforms in contributing to pathological processes resulting from TBI by utilizing mice deficient in COX-1 or COX-2.

RESULTS: Following a mild controlled cortical impact injury, …

Jnk1 Activation Predicts The Prognostic Outcome Of The Human Hepatocellular Carcinoma, Qingshan Chang, Jianguo Chen, Kevin J. Beezhold, Vince Castranova, Xianglin Shi, Fei Chen

Toxicology and Cancer Biology Faculty Publications

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide with an extremely poor prognosis. The classification of HCC based on the molecular signature is not well-established.

RESULTS: In the present study, we reported HCC signature genes based on the JNK1 activation status in 31 HCC specimens relative to the matched distal noncancerous liver tissue from 31 patients. The HCCs with high JNK1 (H-JNK1) and low JNK1 (L-JNK1) were sub-grouped. Two different signature gene sets for both H-JNK1 and L-JNK1 HCC were identified through gene expression profiling. A striking overlap of signature genes was observed between the H-JNK1 …

Integration Of Irradiation With Cytoplasmic Incompatibility To Facilitate A Lymphatic Filariasis Vector Elimination Approach, Corey L. Brelsfoard, William St. Clair, Stephen L. Dobson

Entomology Faculty Publications

BACKGROUND: Mass drug administration (MDA) is the emphasis of an ongoing global lymphatic filariasis (LF) elimination program by the World Health Organization, in which the entire 'at risk' human population is treated annually with anti-filarial drugs. However, there is evidence that the MDA strategy may not be equally appropriate in all areas of LF transmission, leading to calls for the augmentation of MDA with anti-vector interventions. One potential augmentative intervention is the elimination of vectors via repeated inundative releases of male mosquitoes made cytoplasmically incompatible via an infection with Wolbachia bacteria. However, with a reduction in the vector population size, …

Muc1 Is A Downstream Target Of Stat3 And Regulates Lung Cancer Cell Survival And Invasion, Jingchun Gao, Matthew J. Mcconnell, Bin Yu, Jiannong Li, Justin M. Balko, Esther P. Black, Joseph O. Johnson, Mark C. Lloyd, Soner Altiok, Eric B. Haura

Pharmaceutical Sciences Faculty Publications

Signal transducer and activator of transcription 3 (STAT3) is aberrantly activated in human cancer including lung cancer and has been implicated in transformation, tumorigenicity, and metastasis. One putative downstream gene regulated by Stat3 is MUC1 which also has important roles in tumorigenesis. We determined if Stat3 regulates MUC1 in lung cancer cell lines and what function MUC1 plays in lung cancer cell biology. We examined MUC1 expression in non-small cell lung cancer (NSCLC) cell lines and found high levels of MUC1 protein expression associated with higher levels of tyrosine phosphorylated STAT3. STAT3 knockdown downregulated MUC1 expression whereas constitutive STAT3 expression …

Cd19 Signaling Is Impaired In Murine Peritoneal And Splenic B-1 B Lymphocytes, Trivikram Dasu, Vishal Sindhava, Stephen H. Clarke, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

B-1 cells reside predominantly within the coelomic cavities, tonsils, Peyer's patches, spleen (a minor fraction – ∼5%) and are absent in the lymph nodes. They are the primary sources of natural IgM in the body. B-1 cells express polyreactive B cell receptors (BCRs) that cross react with self-antigens and are thus implicated in auto-immune disorders. Previously, we reported that peritoneal B-1 cells are deficient in CD19-mediated intracellular signals leading to Ca²⁺ mobilization. Here, we find that splenic B-1 cells, like peritoneal B-1 cells, are defective in Ca²⁺ release upon B cell activation by co-cross-linking BCR and CD19. In …

Solid Waste Driver Dies After Single Vehicle Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Motor Vehicle

On the morning of a fall day in 2008, a 59 year-old male solid waste worker (Driver 1) entered the cab of a company solid waste truck parked in his driveway and drove to a restaurant a few miles away. After leaving the restaurant, the driver was observed weaving by another solid waste driver (Driver 2) at least two times in the northbound lane of a two-lane state highway. After he had driven 1.8 miles in a little over 2 minutes away from the restaurant, the driver entered a right-hand curve in the highway. While in the curve, the driver …

On Quality Control Measures In Genome-Wide Association Studies: A Test To Assess The Genotyping Quality Of Individual Probands In Family-Based Association Studies And An Application To The Hapmap Data, David W. Fardo, Iuliana Ionita-Laza, Christoph Lange

Biostatistics Faculty Publications

Allele transmissions in pedigrees provide a natural way of evaluating the genotyping quality of a particular proband in a family-based, genome-wide association study. We propose a transmission test that is based on this feature and that can be used for quality control filtering of genome-wide genotype data for individual probands. The test has one degree of freedom and assesses the average genotyping error rate of the genotyped SNPs for a particular proband. As we show in simulation studies, the test is sufficiently powerful to identify probands with an unreliable genotyping quality that cannot be detected with standard quality control filters. …

Evolution Of A Subtilisin-Like Protease Gene Family In The Grass Endophytic Fungus Epichloë Festucae, Michelle K. Bryant, Christopher L. Schardl, Uljana Hesse, Barry Scott

Plant Pathology Faculty Publications

BACKGROUND: Subtilisin-like proteases (SLPs) form a superfamily of enzymes that act to degrade protein substrates. In fungi, SLPs can play either a general nutritive role, or may play specific roles in cell metabolism, or as pathogenicity or virulence factors.

RESULTS: Fifteen different genes encoding SLPs were identified in the genome of the grass endophytic fungus Epichloë festucae. Phylogenetic analysis indicated that these SLPs belong to four different subtilisin families: proteinase K, kexin, pyrolysin and subtilisin. The pattern of intron loss and gain is consistent with this phylogeny. E. festucae is exceptional in that it contains two kexin-like genes. Phylogenetic analysis …

Lipid Nanoparticles With Accessible Nickel As A Vaccine Delivery System For Single And Multiple His-Tagged Hiv Antigens, Weili Yan, Anekant Jain, Ronan O'Carra, Jerold Woodward, Wenxue Li, Guanhan Li, Avindra Nath, Russell J. Mumper

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Lipid-based nanoparticles (NPs) with a small amount of surface-chelated nickel (Ni-NPs) were developed to easily formulate the HIV his-tagged Tat protein, as well as to formulate and co-deliver two HIV antigens (his-p24 and his-Nef) on one particle. Female BALB/c mice were immunized by s.c. injection with his-Tat/Ni-NP formulation (1.5 μg Tat-his/mouse) and control formulations on day 0 and 14. The day 28 anti-Tat specific IgG titer with his-Tat/Ni-NP was significantly greater than that with Alum/his-Tat. Furthermore, splenocytes from his-Tat/Ni-NP immunized mice secreted significantly higher IFN-γ than those from mice immunized with Alum/his-Tat. Although Ni-NPs did not show better adjuvant activity …

Programmable Multi-Dose Intranasal Drug Delivery Device, Daniel Wermeling, Ryan Vallance, Aravind Balasubramanian, Bruce L. Walcott, John A. Main, James E. Lumpp

Pharmacy Practice and Science Faculty Patents

An apparatus and method for the self-administration of a plurality of doses of an intranasal liquid pharmaceutical composition, including opioid analgesics, that includes a drug delivery device containing a plurality of sealed vials, each vial containing a predetermined volume of the pharmaceutical composition, a pump assembly for conveying the liquid pharmaceutical composition from the interior of the vial and discharging it as a nasal spray in response to manual activation by the patient, and programmable means for sequentially advancing a vial to the ready position after passage of a prescribed time interval following the last activation of the delivery device.

Dna-Binding By Haemophilus Influenzae And Escherichia Coli Ybab, Members Of A Widely-Distributed Bacterial Protein Family, Anne E. Cooley, Sean P. Riley, Keith Kral, M. Clarke Miller, Edward Demoll, Michael G. Fried, Brian Stevenson

Microbiology, Immunology, and Molecular Genetics Faculty Publications

BACKGROUND: Genes orthologous to the ybaB loci of Escherichia coli and Haemophilus influenzae are widely distributed among eubacteria. Several years ago, the three-dimensional structures of the YbaB orthologs of both E. coli and H. influenzae were determined, revealing a novel "tweezer"-like structure. However, a function for YbaB had remained elusive, with an early study of the H. influenzae ortholog failing to detect DNA-binding activity. Our group recently determined that the Borrelia burgdorferi YbaB ortholog, EbfC, is a DNA-binding protein. To reconcile those results, we assessed the abilities of both the H. influenzae and E. coli YbaB proteins to bind DNA …

Method And System For In Situ Spectroscopic Evaulation Of An Object, Robert A. Lodder

Pharmaceutical Sciences Faculty Patents

A method and system for spectroscopically determining surface and product characteristics is employed for rapid detection of product characteristics and/or the presence or absence of suspected analytes, and the concentration of the analyte. The method and system uses a signal wide band detector that does not require focusing optics in many environments. It can be used for cleaning validation of pharmaceutical products and process equipment.

Objective And Subjective Residential Context And Urban Children’S Weight Status And Physical And Sedentary Activities, Rachel Tolbert Kimbro, Jeanne Brooks-Gunn, Sara Mclanahan

University of Kentucky Center for Poverty Research Discussion Paper Series

This paper fills this gap by addressing two research questions: first, we ask, are the activity patterns (outdoor play and television watching) of five-year-old children living in large cities associated with children’s weight status? Second, we ask, is residential context, and neighborhood safety in particular, associated with children’s activity patterns? Consistent with past research, we find that outdoor play is negatively associated with weight status, while television watching is positively associated with weight status. We also find, unexpectedly, that the poorest children are playing outdoors the most and watching the most television. Finally, we find that three measures of residential …

Race Disparities In Birth Outcomes In The U.S. South And The Rest Of The Nation, Lenna Nepomnyaschy

University of Kentucky Center for Poverty Research Discussion Paper Series

There are well-documented and as yet unexplained disparities in birth outcomes by race in the United States, even after controlling for socioeconomic status. This paper examines the sources of disparities in low birth weight between blacks and whites in the U.S., by focusing on differences in disparities between two very distinct geographic areas, the Deep South and the rest of the country. Two findings from prior research drive the analyses: First, health overall is worse in the Deep South states; Second, race disparities are smaller in the Deep South than in the rest of the nation. A number of potential …

Violence And Women's Mental Health: The Pain Unequalled, Part One, Carol E. Jordan

Office for Policy Studies on Violence Against Women Publications

No abstract provided.

The North American Transportation Security Center – Serri Analysis Update, Steven Douglas Kreis, Michael M. Barclay

Kentucky Transportation Center Research Report

Executive Summary

There are over 800,000 hazardous materials (hazmat) shipments over the nation’s roads each day. According to the U.S. Department of Homeland Security (DHS), terrorist activity related to the transportation of hazardous materials represents a significant threat to public safety and the nation’s critical infrastructure. Specifically, the federal government has identified the government’s inability to track hazmat shipments on a real-time basis as a significant security vulnerability.

In 2004, the U.S. Federal Motor Carrier Safety Administration (FMCSA) completed a study to determine if “smart truck” technology such as GPS tracking, wireless modems, panic buttons, and on-board computers could be …

A Place At The Blackboard: Including Lesbian, Gay, Bisexual, Transgender, Intersex, & Queer/Questioning Issues In The Education Process, Todd A. Savage, Debra A. Harley

Early Childhood, Special Education, and Counselor Education Faculty Publications

We know from history that lesbian, gay, bisexual, and transgender people have always existed in society (Campos, 2003; Sullivan, 2003). Lesbian, gay, bisexual, transgender, intersexed, and queer/questioning (LGBTIQ) individuals, collectively known as sexual minorities, represent approximately 10% of the population. As many as nine students in every classroom of 30 are in some measure affected by sexual minority issues (e.g., having a gay or lesbian relative or being gay oneself) (AFSC Gay/Lesbian Youth Program, 1991). “Yet even with this substantial number, the code of silence in our nation’s school systems concerning homosexuality remains” (Fontaine, 1997, pp. 101-102).

Carolus Linnaeus (Carl Von Linné), 1707-78: The Swede Who Named Almost Everything, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

No abstract provided.

Georges-Louis Leclerc, Compte De Buffon (1701-1788): The French Rival Of Linnaeus, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

No abstract provided.

Vegf-A As An Inhibitor Of Angiogenesis And Methods Of Using Same, Jayakrishna Ambati

Ophthalmology and Visual Science Faculty Patents

The invention relates to methods and compositions for the treatment or prevention of ocular angiogenesis and neovascularization associated with neovascular disease. Administration of vascular endothelial growth factor (VEGF)-A into the eye when macrophage infiltration is reduced inhibits ocular angiogenesis.

Housing Affordability In Lexington, Kentucky, Christopher Jepsen, Kenneth R. Troske, Nola Ogunro

CBER Research Report

Excerpt from the executive summary:

The rapid rise in housing prices that occurred between 1990 and 2006 led many communities, including Lexington, Kentucky, to become concerned about whether individuals who hold “typical” jobs—such as teachers and police officers—could continue to live in the communities where they worked. Unfortunately, given the lack of recent detailed studies examining the affordability of housing in Fayette County (which contains Lexington), it is hard to know whether concerns about affordable housing are justified. In response to this lack of information, the Home Builders Association of Lexington and the Lexington-Bluegrass Association of Realtors (LBAR) requested that …

T10b9 Monoclonal Antibody: A Short-Acting Nonstimulating Monoclonal Antibody That Spares Γδ T-Cells And Treats And Prevents Cellular Rejection, Thomas H. Waid, John S. Thompson, Maria Siemionow, Stephen A. Brown

Internal Medicine Faculty Publications

T10B9.1A-31/MEDI-500 is a nonmitogenic immunoglobulin M kappa murine monoclonal antibody (mAb) directed against the alpha-beta (αβ) heterodimer of the T-lymphocyte receptor complex. The hybridoma was first produced by fusing spleen cells from BALB/C mice immunized with human peripheral blood T-lymphocytes with SP2/O-Ag14 mutant myeloma cells. The mAb is produced and purified using multistep ion exchange and molecular sieve chromatography protocols. T10B9 has been used successfully to treat acute cellular rejection in renal transplantation and as an immunosuppression induction agent in heart and simultaneous kidney-pancreas transplantation. Because T10B9 is nonmitogenic and causes minimal cytokine release, both treatment of rejection and induction …

Conservation Of The Glucan Phosphatase Laforin Is Linked To Rates Of Molecular Evolution And The Glucan Metabolism Of The Organism, Matthew S. Gentry, Rachel M. Pace

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: Lafora disease (LD) is a fatal autosomal recessive neurodegenerative disease. A hallmark of LD is cytoplasmic accumulation of insoluble glucans, called Lafora bodies (LBs). Mutations in the gene encoding the phosphatase laforin account for approximately 50% of LD cases, and this gene is conserved in all vertebrates. We recently demonstrated that laforin is the founding member of a unique class of phosphatases that dephosphorylate glucans.

RESULTS: Herein, we identify laforin orthologs in a protist and two invertebrate genomes, and report that laforin is absent in the vast majority of protozoan genomes and it is lacking in all other invertebrate …

Hibernation-Like State Induced By An Opioid Peptide Protects Against Experimental Stroke, Cesar V. Borlongan, Teruo Hayashi, Peter R. Oeltgen, Tsung-Ping Su, Yun Wang

Pathology and Laboratory Medicine Faculty Publications

BACKGROUND: Delta opioid peptide [D-ala2,D-leU5]enkephalin (DADLE) induces hibernation in summer ground squirrels, and enhances preservation and survival of isolated or transplanted lungs and hearts. In the present study, we investigated the protective effect of DADLE in the central nervous system.

RESULTS: Adult Sprague-Dawley rats were pretreated with DADLE (4 mg/kg every 2 h x 4 injections, i.p.) or saline prior to unilateral occlusion of the middle cerebral artery (MCA). Daily behavioral tests revealed that ischemic animals treated with DADLE did not show any significant behavioral dysfunctions compared with saline-treated ischemic animals. Opioid antagonists only transiently inhibited the protective effect of …