Digital Commons Network^™

Effects Of A Short-Term Mindfulness Intervention On Depression And Immune Function, Erin C. Walsh

Theses and Dissertations--Psychology

Pro-inflammatory cytokines have been implicated in the pathophysiology and maintenance of depression. This study investigated the effects of a short mindfulness intervention on pro-inflammatory correlates of depression (IL-6 and TNF-α) and selfreported psychological health. Sixty-four college females were assigned to a four-week mindfulness training group or a contact-control group. Cytokines and psychological health were assessed at baseline, post-treatment, and 3-month follow-up (mindfulness group only). IL-6 and TNF-α significantly decreased from baseline to post-treatment in the mindfulness group only; these changes were sustained at 3-month follow-up. No between-group differences in psychological health emerged. Although reductions in proinflammatory cytokines in the mindfulness …

Making Reproductive Health Meaningful: An Anthropological Study Of Planned Parenthood Personnel In Lexington, Ky, Hannah M. Wohltjen

Theses and Dissertations--Anthropology

This thesis focuses on how reproductive health is made meaningful in the context of a Planned Parenthood clinic in Kentucky. Using ethnographic field methods, including participant observation and semi-structured interviews, the paper explores how staff members negotiate definitions of reproductive health as employees of Planned Parenthood health center. The analysis addresses reproductive health discourse among the clinic staff and how reproductive health is used as a site of intervention. It also explores the sociocultural processes and interactions the staff members engage in at the national and local levels and the role these play in shaping the conceptualization of reproductive health …

The Effect Of Client Self-Disclosure On The Physiological Arousal Of The Therapist, Kristyn M. Blackburn

University of Kentucky Master's Theses

This quantitative study investigated the effect of client self-disclosure on the physiological arousal of the therapist and subsequent ratings of the therapeutic alliance, session smoothness, and session depth. Three therapists and 10 clients participated in a 40-minute videotaped therapy session while being attached to sensors that measured heart rate and skin conductance. The participants completed self-report questionnaires designed to assess the therapeutic alliance and session smoothness and depth immediately following the therapy session. The videotaped therapy sessions were later transcribed and coded by two independent coders for the occurrence of client self-disclosure. Correlation analyses were utilized to determine whether or …

Global Transformations, Local Activism: “New” Unionism’S Engagement With Economic And Health Care Transformation In Urban Central Appalachia, Rebecca Adkins Fletcher

University of Kentucky Doctoral Dissertations

It has long been argued that the organization of the U.S. health care system is shaped by the struggles between capital and labor, and this relationship is of increasing significance today. Transformations from an industrial to a service economy, rising insurance costs, neoliberal social policies, and decreased labor union power have increased the number of Americans with reduced access to health care, especially for service workers and women. This dissertation is an ethnographic study of how workers in two leading unions in the “new” unionism movement, the Retail, Wholesale, and Distribution Service Union (RWDSU) and the United Steelworkers (USW) in …

Role Of Angiotensin Converting Enzyme 2 (Ace2) In Obesity-Associated Hypertension, Manisha Gupte

University of Kentucky Doctoral Dissertations

The purpose of this research was to determine whether adipocytes express ACE2 and its role in obesity-associated hypertension with diet-induced obesity.

To determine if ACE2 was expressed in adipose tissue and its regulation in the setting of diet-induced obesity, we fed male mice either a low fat (LF) or high fat (HF) diet acutely (1 week) or chronically ( 4 months). We demonstrated that ACE2 was regulated specifically in adipose tissue with consumption of a HF diet. However, with chronic HF feeding adipose ACE2 was dysregulated resulting in activation of the systemic RAS and increased blood pressure.

To determine the …

Character Education And 4-H Youth Development, Jessica Lynn Mullins

University of Kentucky Master's Theses

Character education assumes responsibility for influencing and educating youth on morals, values, beliefs and positive character attributes in order to influence them into being positive and contributing members of society. Character education is most often taught in the home, schools and community. One community organization that integrates character education into its programming is 4-H Youth Development. The purpose of this research is to view the relationships between character education and 4-H, and more so whether Kentucky 4-H programs influence the knowledge, attitudes and practices of positive character among youth leaders. This study also looked at length of 4-H involvement and …

“All Must Combine In The Struggle Against The Microbes” Global Biopolitics And Twentieth-Century Health Organizations, Patrick Kothe

University of Kentucky Master's Theses

The following paper explores the rise of global biopolitics by focusing on the League of Nations Health Organization (LNHO) and the World Health Organization (WHO) as pivot points around which an international system transitioned into a global system. The central thesis of the paper is that the LNHO served as the first true site of deployment for global discourses on health and hygiene, not as recent scholarship has suggested, the WHO. The purpose of the paper, however, is to provide an overview of the larger transformation of public health in the twentieth century, beginning with the proliferation of nineteenth-­‐century international …

Comparison Of Muscle Activation And Kinematics During The Deadlift Using A Double‐Pronated And Overhand/Underhand Grip, Luke Allen Beggs

University of Kentucky Master's Theses

This study examined muscle activation and relative joint angles during a conventional deadlift while using either a double‐pronated or overhand/underhand (OU) grip. Ten weight‐trained individuals performed the deadlift with 60% and 80% of their 1‐repetition maximum, with three different grip variations. EMG recordings were taken of the left and right biceps brachii, brachioradialis, upper trapezius, and upper latissimus dorsi. Motion capture was used to measure angles of the wrist, elbow, knee, and hip. With an OU grip, significant bilateral asymmetry was seen in EMG activity of biceps brachii and brachioradialis. Mean wrist and elbow angle also showed significant bilateral asymmetry …

Everyday Speech Production Assessment Measure (E-Spam): Reliability And Validity, Tracy N. Watts

University of Kentucky Master's Theses

Purpose: The Everyday Speech Production Assessment Measure (E-SPAM) is a novel test for assessing changes in clients‟ speech production skills after intervention. This study provides information on reliability and validity for the test and overviews its clinical application.

Method & Procedures: E-SPAM, oral reading, and sequential motion rate tasks were administered to 15 participants with motor speech disorders (MSDs). E-SPAM responses were scored using a 5-point system by four graduate students to assess inter-scorer and temporal reliability and to determine validity for E-SPAM.

Results: Findings of this study indicate that the E-SPAM can be scored with sufficient …

Becoming Bodies: How Preadolescent Girls Consume And Produce Media In 21St Century America, Margaret Louise Mcgladrey

University of Kentucky Master's Theses

This study investigates preadolescent girls’ interpretations of images of and messages about women’s bodies presented in both traditional and online media in the American cultural context. Using qualitative methods including in-depth interviews, email diaries, and digital photo collages, this study gives voice to girls aged nine to eleven from diverse racial and socioeconomic backgrounds so that they might tell their stories about interacting with media that is relevant to their relationships with their bodies. Employing objectification theory as well as concepts from the cultural studies tradition, the findings suggest that the process of becoming a female body in the 21 …

What Now? What Next? A Narrative Analysis Of Cross-Cultural Adaptation And College Student Retention, Jason Matthews Martin

University of Kentucky Doctoral Dissertations

First year college student retention is important to colleges and universities nationwide (Bean, 2005). Most of the research on retention focuses on self-report data collected from students after they withdraw from the institution. The present study focuses, instead, on student stories about school, as well as at and about “home” during their first semester.

The experiences of students who transition from high school to college are sometimes likened to those of individuals who enter a new culture for the first time. Thus, this dissertation is grounded in cross-cultural adaptation theory (Kim, 1988, 2001), which posits that successful adaptation occurs via …

Structures, Roles And Relationships Within Public Health’S Response To The 2009-2010 H1n1 Outbreak: The Ties That Bind Public Information Officers And Emergency Risk Communication Efforts, Kathleen G. Vidoloff

University of Kentucky Doctoral Dissertations

Little is known about the role of public health public information officers (PIOs) during public health emergencies. This study uses interpretative methods to learn about the organizational structures that facilitate and constrain emergency risk communication efforts during public health emergencies. Interpretive thematic comparative analysis of PIOs experiences and reflections about their involvement in the 2009-2010 H1N1 response will be used to illustrate how social interactions between and among PIOs, public health staff, and representatives from other agencies create implicit and explicit structures that facilitate and constrain emergency risk communication. The application of three specific concepts from structuration theory, namely, agent, …

Pollution Knowledge And Urban Water Politics In The Ganges River Basin (India), Sya Buryn Kedzior

University of Kentucky Doctoral Dissertations

Millions of people rely upon the Ganges River as a source of water provision and a site of disposal for sewage, solid waste, agricultural runoff and industrial effluent. The river is also a goddess in the Hindu pantheon who is worshipped for her purificatory powers, despite water quality levels that fall far short of standards for use in bathing, washing, and drinking. In recent years, a number of non-governmental organizations (NGOs) have formed to oppose both pollution of the river and the failure of state-run pollution abatement programs. They are joined by an increasingly frequent number of seemingly spontaneous protests …

Atomic Force Microscopy Method Development For Surface Energy Analysis, Clare Aubrey Medendorp

University of Kentucky Doctoral Dissertations

The vast majority of pharmaceutical drug products are developed, manufactured, and delivered in the solid-state where the active pharmaceutical ingredient (API) is crystalline. With the potential to exist as polymorphs, salts, hydrates, solvates, and cocrystals, each with their own unique associated physicochemical properties, crystals and their forms directly influence bioavailability and manufacturability of the final drug product. Understanding and controlling the crystalline form of the API throughout the drug development process is absolutely critical. Interfacial properties, such as surface energy, define the interactions between two materials in contact. For crystal growth, surface energy between crystal surfaces and liquid environments not …

Biological Control Of The Black Cutworm, Agrotis Ipsilon (Lepidoptera: Noctuidae), And Endophyte Mediated Tritrophic Interactions In Turfgrass, Andrea Jeanne Bixby-Brosi

University of Kentucky Doctoral Dissertations

Components of successful pest management programs must be complementary and not antagonistic. This project examined interactions between natural enemies of the black cutworm, Agrotis ipsilon (Hufnagel), an important turfgrass pest, and host plant resistance by endophytic grass.

Agrotis ipsilon nucleopolyhedrovirus (AgipMNPV) was examined as a bio-insecticide for controlling A. ipsilon in turfgrass. Fresh (1-week-old) AgipMNPV residues killed 76−86% of neonates hatching from eggs on golf course tees, however, residual control of implanted larvae lasted no more than a few weeks. Combinations of AgipMNPV with adjuvants, such as optical brightener and lignin, failed to accelerate or extend …

Examining The Role Of Personality, Peers, And The Transition To College On Substance Use, Ursula Louise Bailey

University of Kentucky Doctoral Dissertations

It is well established that there is an increase in substance use among college students. In the literature, this increase in use has been attributed to different personality factors, such as sensation seeking. However, what has not received sufficient attention is the possibility that the new peer groups, afforded by the transition to college, introduce unique influence on the relationship between personality and substance use. The purposes of the current study were to explore whether personality predicted substance use across the transition to college whether peer substance use moderated that relationship.

The current study examined developmentally the relations among personality, …

Circadian And Homeostatic Regulation Of Sleep In Cast/Eij And C57bl/6j Mice, Peng Jiang

University of Kentucky Doctoral Dissertations

Sleep is essential for mammals and possibly for all animals. Advancing our knowledge of sleep regulation is crucial for the development of interventions in sleep-related health and social problems. With this aim, this study utilizes laboratory mice to explore sleep regulatory mechanisms at behavioral, molecular, and genetic levels.

Sleep is regulated by the interaction of circadian and homeostatic processes. The circadian clock facilitates sleep to occur at a favorable time of the day. Normal mice, such as the C57BL/6J (B6) strain, sleep mostly during the day and initiate activities at dark onset. Here, I show mice of the CAST/EiJ (CAST) …

Genetically Engineered Aequorin For The Development Of Novel Bioanalytical Systems, Krystal Teasley Hamorksy

University of Kentucky Doctoral Dissertations

The ability to rationally or randomly modify proteins has expanded their employment in various bioanalytical applications. The bioluminescent protein, aequorin, has been employed as a reporter for decades due to its simplistic, non-hazardous nature and its high sensitivity of detection. More recently aequorin has been subject to spectral tuning. Techniques such as random and site-directed mutagenesis, the incorporation of coelenterazine analogues and the incorporation of non-natural amino acids have expanded the palette of aequorin by altering their emission wavelengths and/or half-lifes. Due to the increased diversity of aequorin, it can be used in multianalyte detection.

Although aequorin has been studied …

Regulation Of Abcg5 And Abcg8 Sterol Transporters In Biliary Cholesterol Elimination, Reverse Cholesterol Transport And Dyslipidemia, Nadezhda Steliyanova Sabeva

University of Kentucky Doctoral Dissertations

ATP-binding cassette transporters ABCA1 and ABCG1 initiate reverse cholesterol transport generating HDL particles, whereas ABCG5/G8 promote biliary cholesterol secretion thereby facilitating the last step of reverse cholesterol transport. Mutations in the leptin axis result in obesity and dyslipidemia in ob/ob and db/db mice. These mice have defective HDL clearance, increased plasma cholesterol and decreased biliary cholesterol elimination. My studies demonstrate that ABCG5/G8 protein is low in these animals and can be restored with caloric restriction or leptin replacement. To directly test whether ABCG5/G8 alone is able to correct reverse cholesterol transport defect, liver specific ABCG5/G8 expression was achieved in db/db …

Restoration Of Tall Fescue Pastures To Native Warm Season Grasslands: Does A Fungal Endophyte Symbiosis Play A Role In Restoration Success?, Sarah Lynn Hall

University of Kentucky Doctoral Dissertations

Tall fescue, a cool-season grass native to Europe, central Asia, and northern Africa, has been widely distributed throughout the U.S. for use as turf and forage. Following its widespread planting, its ability to associate with a toxic fungal endophyte, Neotyphodium coenophialum, was discovered. Research has linked this fescue-endophyte association with increased biotic and abiotic stress resistance in endophyte-infected (E+) versus endophyte-free (E-) plants, and these differences may affect the ability of land managers to eradicate tall fescue and restore native grasslands. I conducted three studies to examine whether E+ tall fescue plants respond differently to management than E- plants, …

Using Linked Household-Level Datasets To Explain Consumer Response To Bse In Canada, Xin Wang

University of Kentucky Doctoral Dissertations

Household-level Canadian meat purchases from 2002-2008, a Food Opinions Survey conducted in 2008 at the national level and household-level egg purchases from 2002-2005 in Alberta and Ontario were used to explore consumer responses to Bovine Spongiform Encephalopathy (BSE) in Canada.

The opinions survey focused on nutritional priorities, general and specific food safety concerns, and trust in government and food industry decision makers. The egg data set contained specific product information allowing us to distinguish purchases of conventional eggs from those of value-added eggs with perceived health attributes. Thus, the egg purchase data appeared to be an interesting proxy of revealed …

Values In Place: Intersecting Values In Rails To Trails Landscapes, Lisa Rainey Brownell

University of Kentucky Doctoral Dissertations

This dissertation is a study of the values and meanings people attach to places and why exploring those values is important in trails and historic preservation planning. From a foundation in critical humanistic geography and values based preservation literatures, the dissertation examines three rails to trails projects as case studies. Primary research questions include: how does a landscape become valued, devalued, and/or revalued through time? In what ways do different values of different people or groups intersect in rails to trails landscapes and how do they shape the landscape? How do historic values intersect with economic, social, political, and other …

Role Of Pi3k-Akt Pathway In The Age Associated Decline In Tlr Mediated Activation Of Innate And Adaptive Immune Responses, Mosoka Papa Fallah

University of Kentucky Doctoral Dissertations

Immunosenescence results in reduced immune response to infections with Streptococcus pneumoniae as well as to pneumococcal polysaccharide vaccines. The antibody response to the capsular polysaccharide (CPS) provides protection against S. pneumoniae infection. CPS immunoresponse is T cell independent and needs the macrophage-derived cytokines such as IL-12, IL-6 and IL-1β to elicit an antibody response. We showed a cytokine dysregulation, i.e. a decrease in IL-12, IL-6 and TNF-α but an increase in IL-10, in the aged (18-24 months old comparable to >65 years in human) compared to young adult mouse (8-12 weeks less than 65 years old) splenic macrophages (SM) or …

Post-Transcriptional Regulation Of Afp And Igm Genes, Lilia M. Turcios

University of Kentucky Doctoral Dissertations

Gene expression can be regulated at multiple steps once transcription is initiated. I have studied two different gene models, the α-Fetoprotein (AFP) and the immunoglobulin heavy chain (IgM) genes, to better understand post-transcriptional gene regulation mechanisms. The AFP gene is highly expressed during fetal liver development and dramatically repressed after birth. There is a mouse strain-specific difference between adult levels of AFP, with BALB/cJ mice expressing 10 to 20-fold higher levels compared to other mouse strains. BALB/cJ mice express low levels of Zhx2 and thus incompletely repress AFP. Despite differences in steady state AFP mRNA levels in the adult liver …

Novel Mechanisms In Inflammatory Bowel Disease, Razvan I. Arsenescu

University of Kentucky Doctoral Dissertations

Inflammatory Bowel Diseases, Crohn's Disease and Ulcerative colitis, are idiopathic chronic conditions with multifactorial determinants. In general, terms, intestinal inflammation results from abnormal host-microbe interactions. Alterations in homeostasis involve host genetic factors, environmental cues and unique luminal microbial niches. We have examined the coordinated expressions of several molecular targets relevant to the mucosal immune system and identified signature biomarkers of IBD. Qualitative and quantitative changes in the composition of microbiota can be related to unique immuno-phenotypes. This in turn can have more systemic effects that involve energy metabolism. Adiponectin, an adipose tissue derived adipokine, can restore cellular ATP levels and …

The Ecology Of Disturbances And Global Change In The Montane Grasslands Of The Nilgiris, South India, Madhusudan P. Srinivasan

University of Kentucky Doctoral Dissertations

Biodiversity rich regions worldwide face threats from various global change agents. This research quantifies environmental influences on vegetation, and the impacts of exotic woody plant invasion and anthropogenic nitrogen (N) deposition in a global biodiversity hotspot. The study was conducted in the montane grasslands of the Nilgiris, Western Ghats, and outlines potential management options for this region. Specifically, I examined (1) the role of environmental factors in influencing native plant distribution and ecosystem properties, (2) the status and impact of exotic shrub (Scotch broom, henceforth broom) invasion, (3) the role of disturbances in the success of broom, (4) the role …

Factors Influencing Community Response To Locally Undesirable Land Uses: A Case Study Of Bluegrass Stockyards, Terry Logan Lunsford

University of Kentucky Doctoral Dissertations

Community development is an ongoing issue that faces communities as they develop. This is a case study where two communities where faced with an identical development proposal involving Bluegrass Stockyards. Bluegrass Stockyards a prominent livestock marketing business, located in Lexington, KY needed to relocate its facility and looked at communities in Lincoln and Woodford County Kentucky as possible new locations.

By looking at the case of Bluegrass Stockyards this study is able to use Conflict Theory, Growth Theory and Frame Analysis to look at the development process and issues that was associated with this development proposal. With the two communities …

Central Neural And Behavioral Correlates Of Voice Secondary To Induced Unilateral Vocal Fold Paralysis, Ashwini Joshi

University of Kentucky Doctoral Dissertations

Understanding the involvement of the central nervous system (CNS) in voice production is essential to incorporating principles of neuroplasticity into therapeutic practice for voice disorders. Early steps to attaining this goal require the identification of specific neural biomarkers of the changes occurring in the CNS from a voice disorder and its subsequent treatment. In the absence of an adequate animal vocalization model, the larynx has not been acutely and reversibly perturbed to concurrently examine the effect on both peripheral and central processing of the altered input/output.

Using a unique, reversible perturbation approach, it was the purpose of this study to …

Conditioning Democratization: Eu Membership Conditionality And Domestic Politics In Balkan Institutional Reforms, Ridvan Peshkopia

University of Kentucky Doctoral Dissertations

The uneven effects of EU membership conditionality on Eastern European reforms continue to puzzle the research community. Sometimes, the research focus has been too large, considering EU membership conditionality as a policy implemented uniformly across policy areas. Other efforts take a too narrow approach by trying to explain the effects of EU membership conditionality in single sectors. I suggest studying this phenomenon through a set of mid-level theories in a cross-country, cross-sectorial approach. I argue that both the intensity of EU membership conditionality and reform outcomes are contingent upon the policy sector context; hence, we should take a sectorial contextual …

Initial Response Of Invasive Exotic Plant Species To Timber Harvesting In Southeastern Kentucky Forests, Kevin Patrick Devine

University of Kentucky Master's Theses

The responses of invasive exotic plant species (IES) to silvicultural treatments one growing season after timber harvesting were examined in the Cumberland Plateau region of Southeastern Kentucky. Treatments included a commercial deferment harvest and unharvested control applied to five watersheds within University of Kentucky’s Robinson Forest. The effects of harvesting were compared between treatments and between preharvest and postharvest samplings. The spatial distribution and abundance of targeted IES throughout forest sites and trail systems were calculated from several sampling schemes. Additional analyses were performed to quantify forest disturbances derived from harvest activities to determine the relationships between soil, light levels, …