Digital Commons Network^™

Rhodococcus Equi Infection And Interferon-Gamma Regulation In Foals, Lingshuang Sun

Theses and Dissertations--Veterinary Science

Rhodococcus equi (R. equi) is one of the most serious causes of pneumonia in young foals. The clinical disease is of great concern to breeding farms worldwide due to the impact of mortality on economic losses. While adult horses are resistant to R. equi, foals exhibit a distinct age-associated susceptibility. The mechanism underlying this susceptibility in foals is not well understood. Interferon-gamma (IFNg) plays an important role in the clearance of R. equi, but its expression is impaired in neonatal foals. Moreover, the regulation of this age-related IFNg expression in foals remains unknown. In humans, IFNg …

Control And Passive Treatment Of Runoff From Horse Muck Storage Structures Using Rain Gardens, Hillary K. Otte

Theses and Dissertations--Forestry and Natural Resources

Runoff from livestock operations may contain a variety of pathogens and high levels of nutrients and other harmful contaminants, and is of particular concern in central Kentucky as watersheds are threatened by waste generated from a high concentration of equine activity. Rain gardens are a type of stormwater management tool used to capture and passively treat runoff. This project aimed to incorporate rain gardens into the horse muck storage structures at a thoroughbred facility in the Canr Run watershed in Lexington, Kentucky. Water quality data from soil water within two rain garden muck pads and two control pads, and grab …

Effects Of Methoprene On The Survivorship Of Adult Aedes Mosquitoes: A Strategy Or Inactivating Released Mosquitoes, Peter J. Brabant Iii

Theses and Dissertations--Entomology

Methoprene is a Juvenile Hormone (JH) analogue commonly used for the control of mosquito larvae. When applied to a mosquito breeding site, methoprene enters the haemolymph, where it mimics the function of JH and interferes with normal metamorphosis, resulting in larval mortality. Methoprene is commonly used for the control of larvae and has not been used as an adulticide, due to an absence of acute effects. This study evaluated possible chronic effects caused by the exposure of adult Aedes mosquitoes to methoprene. Methoprene was applied, in both technical grade and the commercially available Altosid®, topically to adults through droplet application …

An Analysis Of The Influence Of Nutrition Information On Consumers’ Restaurant Menu Choices, Joseph Adam Higgins

Theses and Dissertations--Nutrition and Food Systems

Obesity has risen drastically in the past few decades. Some experts contribute this to an increased prevalence of eating food away from home and consuming large portion sizes. A popular discussion in policy and legislation arenas has been mandatory implementation of nutrition information on restaurant chain menus. Menu labeling in restaurants that have 20 or more locations nationally was first implemented by New York City in an effort to increase consumer awareness of the calorie content of menu entrees. Since New York City’s implementation of restaurant menu labeling in 2008, there have been conflicting studies on whether nutrition menu labeling …

Kentucky Wic Participants’ Knowledge, Attitudes, And Beliefs Regarding Grains, Dustin Tyler Reed

Theses and Dissertations--Nutrition and Food Systems

Obesity, diabetes, cardiovascular disease, and poor dietary habits are major healthcare problems in the United States. These issues are especially prevalent in the state of Kentucky and among at-risk populations such as Women, Infants, and Children (WIC) participants. Studies have found that whole grains play a role in weight maintenance, protection against type 2 diabetes, and lowering cholesterol. Interventions aimed at improving WIC participant dietary behavior and intake has been successful as well. This study assessed Kentucky WIC participants’ knowledge of the benefits of consuming grains, attitudes and beliefs regarding food purchasing and grains, and identified grains consumed in a …

Acculturation Of Asian Indian Women In The United States, Varudhini Kankipati

Theses and Dissertations--Family Sciences

The United States is home to nearly three million Asian Indians. The difference in Asian Indian and American cultures creates a need for Asian Indians to acculturate, upon migration to the U.S. It has been theorized that acculturation becomes harder when the two cultures of contact are dissimilar. Particularly, immigrant women and children have been found to be more vulnerable than men to acculturative stress, where acculturative stress is defined as the psychological impact of adaptation to a new culture. Hence, this study focuses on acculturation of Asian Indian women and specifically on factors influencing their acculturation.

Research findings from …

Obesity Related Perceptions And Practices Among Educators In The Expanded Food And Nutrition Education Program, Poonam Mahajan

Theses and Dissertations--Nutrition and Food Systems

Childhood obesity has increased significantly in the past decade. The same factors putting adults at risk for obesity apply to children as well. For children, the family environment may be one of the largest factors. Obesity affects both adults and children of low socioeconomic status. It also affects families living in the Appalachian region of the United States more frequently than other regions. The purpose of this study was to examine the relationships between obesity related behaviors and nutrition education among Appalachian participants in the Expanded Food and Nutrition Education Program (EFNEP). For this study eleven educators from the Appalachian …

Perception And Usage Of Video Streaming/Electronic Media In Nutrition Education For Kentucky Individuals, Beth Ann Oldiges

Theses and Dissertations--Nutrition and Food Systems

Nutrition Education Professionals (NEP) working for Extension Agents of Kentucky were surveyed to evaluate their use of a variety of tools in nutrition education in three areas: nutrition, food safety, and food preparation. The purpose of this research was to determine the perception and usage of video streaming/electronic media among community nutrition professionals as a means to better educate individuals in Kentucky and furthermore, to determine if demographics of NEP affect their utilization of this technology. The results concluded that regardless of demographics, NEP perceive video streaming/electronic media to be effective in nutrition education. However, age, employment length, and video …

Nutrition Knowledge Of Consumers And Health Professionals And The Use Of Menu Calorie Labeling, Kate Louise Perkins

Theses and Dissertations--Nutrition and Food Systems

The purpose of this study is to assess the need for menu labeling of calories based on the ability of food consumers to identify lower calorie options. By surveying consumers in Kentucky and Ohio and health professionals in Kentucky about their awareness of caloric content, basic nutrition knowledge and ability to choose lower calorie options, we can predict the potential benefit of menu labeling initiatives and the amount of education that will be necessary for consumers to effectively use menu labeling to make informed decisions on calorie intake. Through online surveys, it was determined that consumers were better able to …

Evaluation Of The Body Composition Of Female Collegiate Athletes Using The Bod Pod, Adrienne Jennifer Glodt Baker

Theses and Dissertations--Nutrition and Food Systems

The body composition of female collegiate athletes was measured using the Bod Pod® device. The sample consisted of 75 student athletes, aged 18 to 22 years old. Five sports at the university level were represented, including basketball, gymnastics, soccer, swimming & diving, and soccer. Participants were measured at the preseason and postseason periods. Overall, participants in all five sports were not found to change significantly in total body mass, fat mass, fat free mass, percent body fat, or body mass index from the preseason period to the postseason period at the alpha = 0.05 level. On average, the members from …

Local Ghanaian Stakeholder Perceptions After One Year Of A New School Feeding Program In Adjeikrom, Ghana, Monica E. Fowler

Theses and Dissertations--Nutrition and Food Systems

The objective of this qualitative longitudinal study was to assess stakeholder perceptions after one year of a new school feeding program to contrast and compare to pre-program expectations. Semi-structured interviews were conducted using questions reviewed by an in-country collaborator for cultural sensitivity and appropriateness. Follow up questions were determined from participant response. The interviews were analyzed by performing content analysis using open and axial coding to find key concepts in the data. The results showed the school feeding program met stakeholder expectations regarding reduction in absenteeism and truancy, improving classroom behavior and increasing enrollment as well as relieving short term …

Cross-Cultural Comparison Of Perceptions About School Feeding Programs From Ghanaian And U.S. Adults, Yolanda L. Jackson

Theses and Dissertations--Nutrition and Food Systems

Because of an increase in food insecurity over the past decade, the U.S. and many developing countries are focusing efforts on issues relating to child hunger. School feeding programs have been in existence for roughly 220 years but modifications have been made over the years to improve the quality of food they serve. Little research has been conducted about adult perceptions of school feeding programs and the impact it has on the community in the U.S. as well as developing countries such as Ghana, West Africa. Because of the gap in literature on school feeding programs and adult perceptions about …

Influence Of Medical Marijuana Programs On Controlled Substance Utilization, James A. Farley Ii

MPA/MPP/MPFM Capstone Projects

Currently there are no studies examining the impact of medical cannabis programs on controlled substance prescribing. The intent of this study is to conduct a preliminary investigation into whether cannabis substitution for prescription drugs results in lower rates of controlled substance use. The hypothesis is that controlled substance use (in this project, defined by controlled substance supply) will decline in states after the introduction of medical cannabis programs.

State and year–specific per capita retail distributions of the seven most commonly prescribed opioid drugs (fentanyl, hydrocodone, hydromorphone, meperidine, methadone, morphine, and oxycodone) were obtained from the Automation of Reports and Consolidated …

Strategic Management And Job Satisfaction: Agency-Level Effects In The Federal Government, Tara Bopp

MPA/MPP/MPFM Capstone Projects

As strategic planning and human capital strategies become more regulated in the United States government, a decision must be made by federal agency heads. Will agencies fully participate and buy into the process of participative strategic planning and management or will they comply with regulations and reforms merely for the sake of compliance with little further effort? The intent of this study is to show that agencies that choose to do more than simply comply with GPRA and implement strategic planning and management can benefit from their increased effort in by increasing job satisfaction in their workforce.

The Government Performance …

Influence Of Prescription Drug Monitoring Programs On Acetaminophen-Related Liver Toxicity, Aaron Barcelo

MPA/MPP/MPFM Capstone Projects

No executive summary.

An Analysis Of Laboratory Inspections At The University Of Kentucky, Rachel Eldridge

MPA/MPP/MPFM Capstone Projects

Recent news stories about laboratory accidents in which students were severely injured and killed have brought much-needed attention to lab safety. Creating a positive safety climate on college and university campuses is very important in reducing accidents. At the University of Kentucky, all laboratories are inspected once every year, and the results are recorded in a lab inspection database. These data include the department, building, lab classification, type of violation, room number, and the name of the PI (Principal Investigator). I want to analyze inspections to find out whether department and the lab classification are significant in looking at violations. …

Can Drug Court Results Be Predicted? Completion And Termination Of Drug Court Participants, Christopher D. Geisler

MPA/MPP/MPFM Capstone Projects

The substantial growth in Kentucky’s prison population since the 1980s has resulted in increased spending on corrections, both the total amount and as a percentage of total general fund spending. The sustained growth in corrections spending combined with shrinking budgetary realities has created an incentive to explore ways to reduce costs without compromising public safety. Community supervision programs are one alternative to addressing this problem. They are an attractive approach to dealing with certain types of criminals because the cost is significantly lower than incarceration. Drug courts are an example of community supervision that allows drug offenders to avoid imprisonment …

Corporate Income Tax Credits: Examining The Relationship Between Tax Credits And Employment, Elena Yatskovskaya

MPA/MPP/MPFM Capstone Projects

Tax incentives - in particular, corporate tax credits - have been a prominent topic of discussion among government administrators, public economists, and political scholars. This capstone project will contribute to the discussion by addressing the relationship between tax credits and employment, and provide an empirical analysis to aid in determining the effect that tax credits may have on employment.

The study uses aggregate data spanning ten years, from 1999 to 2008, and representing 18 industrial sectors with 180 observations. The data are analyzed with the help of the two panel data regression models: Fixed Effects and Between Effects. The …

Federal Tax Policy And Charitable Giving: Revisiting The 1985 Study By Charles T. Clotfelter, Mary Catherine Tinnon

MPA/MPP/MPFM Capstone Projects

This study was inspired and given its basic research design by a study conducted in 1985 by Charles T. Clotfelter, which sought to explain charitable giving by both individuals and corporations in relation to federal tax policy. Clotfelter determined that federal tax policy did influence charitable giving by corporations. The issue to be addressed here is, do the Clotfelter findings still hold true today?

The focus of this current study is on charitable giving by corporations only and the dataset was built from the same IRS Statistics of Income that Clotfelter used. Additional data were added from the Bureau of …

Soil Microbial Community Response To Climate Change: Results From A Temperate Kentucky Pasture, Lindsey C. Slaughter

Theses and Dissertations--Plant and Soil Sciences

Climate change is likely to alter plant species composition and interactions between plants and soil microbes that together dictate the quantity and quality of forage produced in pastures, the base of animal production in central Kentucky. This study assessed the seasonal dynamics of soil microbes and their response to increased temperature (+3^oC) and growing season precipitation (+30% of the mean annual). Total soil microbial biomass, community composition, enzyme activities, potential carbon mineralization, and catabolic responses to selected substrates were measured seasonally in the different climate treatments. In this system, seasonal variability was a dominant driving factor for all …

Neuroinflammation In The Aging Down Syndrome Brain; Lessons From Alzheimer's Disease, Donna M. Wilcock

Physiology Faculty Publications

Down syndrome (DS) is the most genetic cause of mental retardation and is caused by the triplication of chromosome 21. In addition to the disabilities caused early in life, DS is also noted as causing Alzheimer's-disease-like pathological changes in the brain, leading to 50-70% of DS patients showing dementia by 60-70 years of age. Inflammation is a complex process that has a key role to play in the pathogenesis of Alzheimer's disease. There is relatively little understood about inflammation in the DS brain and how the genetics of DS may alter this inflammatory response and change the course of disease …

"Human Babesiosis": An Emerging Transfusion Dilemma, Helieh S. Oz, Karin N. Westlund

Physiology Faculty Publications

Babesiosis, a common disease of animals, can infect humans via vector "tick bite", particularly in endemic areas. The recent reports of fatal cases in Hepatitis C and postliver transplant patients resulting from transfusion of contaminated blood should alert the medical profession regarding this emerging dilemma in endemic as well as nonendemic areas and the need for accurate blood screening for transfusion. Here, we illustrate different stages of the parasite lifecycle, progression of babesiosis in animal model, some aspects of pathologic outcomes, ongoing therapeutic modalities, and a feasible Acridine Orange fluorescent methodology for the diagnostic evaluation of blood samples.

Nutrition, Inflammation, And Infectious Disease, Helieh S. Oz, Sung-Ling Yeh

Physiology Faculty Publications

No abstract provided.

Pegylated Arginine Deiminase Downregulates Colitis In Murine Models, Helieh S. Oz, Jian Zhong, Willem J. S. De Villiers

Physiology Faculty Publications

Arginine deiminase (ADI), an arginine-metabolizing enzyme involved in cell signaling, is dysregulated in multiple inflammatory diseases and cancers. We hypothesized that pegylated ADI (ADI-PEG) provide protection against colitis.

METHODS: Dextran sodium sulfate colitis was induced in IL-10-deficient and BALB/c (WT) mice. ADI-PEG was administered i.p., and inflammatory mediators and pathology were evaluated.

RESULTS: Acute colitis in mice was manifested by increases in inflammatory biomarkers, such as serum amyloid A (SAA, P < 0.001), IL-12 p40, and disease index (3-Fold). In contrast, ADI-PEG significantly decreased clinical disease index, SAA levels, and inflammatory cytokines in blood as well as in colonic explants. Animals developed moderate (2.2 ± 0.3 WT) to severe (3.6 ± 0.5 IL-10 deficient) colonic pathology; and ADI-PEG treatment significantly improved the severity of colitis (P < 0.05). Marked infiltration of CD68+ macrophages and iNOS expression were detected in colonic submucosa in colitic animals but not detected in ADI-PEG-treated animals.

CONCLUSION: ADI-PEG attenuated inflammatory responses by suppression of macrophage infiltration and iNOS expression in colitic animals. ADI-PEG can serve as a potential therapeutic value in IBD.

Mild Cognitive Impairment: Statistical Models Of Transition Using Longitudinal Clinical Data, Erin L. Abner, Richard J. Kryscio, Gregory E. Cooper, David W. Fardo, Gregory A. Jicha, Marta S. Mendiondo, Peter T. Nelson, Charles D. Smith, Linda J. Van Eldik, Lijie Wan, Frederick A. Schmitt

Sanders-Brown Center on Aging Faculty Publications

Mild cognitive impairment (MCI) refers to the clinical state between normal cognition and probable Alzheimer's disease (AD), but persons diagnosed with MCI may progress to non-AD forms of dementia, remain MCI until death, or recover to normal cognition. Risk factors for these various clinical changes, which we term "transitions," may provide targets for therapeutic interventions. Therefore, it is useful to develop new approaches to assess risk factors for these transitions. Markov models have been used to investigate the transient nature of MCI represented by amnestic single-domain and mixed MCI states, where mixed MCI comprised all other MCI subtypes based on …

Development And Deployment Of A Bioreactor For The Removal Of Sulfate And Manganese From Circumneutral Coal Mine Drainage, C. B. Mastin, J. D. Edwards, Christopher D. Barton, Anastasios D. Karathanasis, Carmen T. Agouridis, Richard C. Warner

Forestry and Natural Resources Faculty Publications

Surface mining, in the form of contour mining and mountain-top removal, is a common means for retrieving coal in the Appalachian Coal Belt region of Kentucky. Overburden or excess spoil generated by these two methods is placed in valley fills. Traditionally Constructed fills have been shown to adversely impact headwater ecosystems via stream burial and through alterations to the hydrology, sediment supply, water quality and biological composition of downstream reaches. Mine drainages emanating from the toe of valley fills often contain elevated levels of total dissolved solids and heavy metals. Drainage chemistry from Guy Cove, a valley fill located in …

Equitable Consequences? Issues Of Evidence, Equity And Ethics Arising From Outdoor Smoke-Free Policies, Ann Pederson, Wendy Rice, Phoebe M. Long, Natasha Jategaonkar, Lorraine Greaves, Steven Chasey, Natalie Hemsing, Chizimuzo T.C. Okoli, Joan L. Bottorff

Nursing Faculty Publications

From Introduction:

Kass argues that an ethical approach in public health is one that places the fewest burdens on individuals’ health without significantly reducing the potential benefits of intervening. Yet many population health regulations are highly intrusive, compromising individual liberty and imposing penalties for non-compliance. Moreover, the benefits of these regulations and the burdens they impose may not be shared equally. When developing interventions, the state has, therefore, an obligation to consider the benefits and burdens, particularly on those vulnerable to health inequities and other disparities.

Privacy Preserving Distributed Data Mining, Zhenmin Lin

Theses and Dissertations--Computer Science

Privacy preserving distributed data mining aims to design secure protocols which allow multiple parties to conduct collaborative data mining while protecting the data privacy. My research focuses on the design and implementation of privacy preserving two-party protocols based on homomorphic encryption. I present new results in this area, including new secure protocols for basic operations and two fundamental privacy preserving data mining protocols.

I propose a number of secure protocols for basic operations in the additive secret-sharing scheme based on homomorphic encryption. I derive a basic relationship between a secret number and its shares, with which we develop efficient secure …

C6 Pyridinium Ceramide Influences Alternative Pre-Mrna Splicing By Inhibiting Protein Phosphatase-1, Chiranthani Sumanasekera, Olga Kelemen, Monique Beullens, Brandon E. Aubol, Joseph A. Adams, Manjula Sunkara, Andrew J. Morris, Mathieu Bollen, Athena Andreadis, Stefan Stamm

Molecular and Cellular Biochemistry Faculty Publications

Alternative pre-mRNA processing is a central element of eukaryotic gene regulation. The cell frequently alters the use of alternative exons in response to physiological stimuli. Ceramides are lipid-signaling molecules composed of sphingosine and a fatty acid. Previously, water-insoluble ceramides were shown to change alternative splicing and decrease SR-protein phosphorylation by activating protein phosphatase-1 (PP1). To gain further mechanistical insight into ceramide-mediated alternative splicing, we analyzed the effect of C6 pyridinium ceramide (PyrCer) on alternative splice site selection. PyrCer is a water-soluble ceramide analog that is under investigation as a cancer drug. We found that PyrCer binds to the PP1 catalytic …

The American Recovery And Reinvestment Act Of 2009: An Investigation Into The Determinants Of Funds Awarded To The States, Kaitlin Bromann

MPA/MPP/MPFM Capstone Projects

The American Recovery and Reinvestment Act of 2009 is one of the largest government responses to an economic crisis in the history of the United States. The purpose of this paper is to examine the determinants of the total funds awarded to states by federal agencies in the Recovery Act. A review of budgetary theory, distributive politics, and electoral vote maximization theory provides context of historical determinants of resource allocation by the federal government.

Then from this literature I develop a model including economic and political variables. The dependent variable in my model is the total funds awarded to the …