Digital Commons Network^™

Prostate Cancer-Specific And Potent Antitumor Effect Of A Dd3-Controlled Oncolytic Virus Harboring The Pten Gene, Miao Ding, Xin Cao, Hai-Neng Xu, Jun-Kai Fan, Hong-Ling Huang, Dong-Qin Yang, Yu-Hua Li, Jian Wang, Runsheng Li, Xin-Yuan Liu

Molecular and Cellular Biochemistry Faculty Publications

Prostate cancer is a major health problem for men in Western societies. Here we report a Prostate Cancer-Specific Targeting Gene-Viro-Therapy (CTGVT-PCa), in which PTEN was inserted into a DD3-controlled oncolytic viral vector (OV) to form Ad.DD3.E1A.E1B(Δ55)-(PTEN) or, briefly, Ad.DD3.D55-PTEN. The woodchuck post-transcriptional element (WPRE) was also introduced at the downstream of the E1A coding sequence, resulting in much higher expression of the E1A gene. DD3 is one of the most prostate cancer-specific genes and has been used as a clinical bio-diagnostic marker. PTEN is frequently inactivated in primary prostate cancers, which is crucial for prostate cancer progression. Therefore, the Ad.DD3.D55-PTEN …

Acupuncture: The New Ancient, Connie Jennings

Year of China Events

No abstract provided.

Genetics Of Clusterin Isoform Expression And Alzheimer's Disease Risk, I-Fang Ling, Jiraganya Bhongsatiern, James F. Simpson, David W. Fardo, Steven Estus

Sanders-Brown Center on Aging Faculty Publications

The minor allele of rs11136000 within CLU is strongly associated with reduced Alzheimer's disease (AD) risk. The mechanism underlying this association is unclear. Here, we report that CLU1 and CLU2 are the two primary CLU isoforms in human brain; CLU1 and CLU2 share exons 2-9 but differ in exon 1 and proximal promoters. The expression of both CLU1 and CLU2 was increased in individuals with significant AD neuropathology. However, only CLU1 was associated with the rs11136000 genotype, with the minor "protective" rs11136000T allele being associated with increased CLU1 expression. Since CLU1 and CLU2 are predicted to encode intracellular and secreted …

Genetic Association Studies Of Copy-Number Variation: Should Assignment Of Copy Number States Precede Testing?, Patrick Breheny, Prabhakar Chalise, Anthony Batzler, Liewei Wang, Brooke L. Fridley

Biostatistics Faculty Publications

Recently, structural variation in the genome has been implicated in many complex diseases. Using genomewide single nucleotide polymorphism (SNP) arrays, researchers are able to investigate the impact not only of SNP variation, but also of copy-number variants (CNVs) on the phenotype. The most common analytic approach involves estimating, at the level of the individual genome, the underlying number of copies present at each location. Once this is completed, tests are performed to determine the association between copy number state and phenotype. An alternative approach is to carry out association testing first, between phenotype and raw intensities from the SNP array …

Positive Youth Development Constructs: Conceptual Review And Application, Daniel T. L. Shek, Rachel C. F. Sun, Joav Merrick

Pediatrics Faculty Publications

No abstract provided.

Sex And Gender In Tobacco Reduction - The State Of The Evidence, Iris Torchalla, Chizimuzo T.C. Okoli

Nursing Presentations

• Global prevalence, risk, and mortality from smoking

• Gender/Sex specific diseases associated with tobacco use

• Gender/Sex specific reasons for smoking

• Gender/Sex specific factors in treating tobacco use

The Tobacco Dependence Clinic: Gender Issues And Possibilities In Cessation Treatment, Chizimuzo T.C. Okoli, Milan Khara

Nursing Presentations

• The Tobacco Dependence Clinic (TDC) is a program that provides behavioural counselling and up to 6-7months of no-cost pharmacotherapy for clients through VCH Mental Health and Addiction Services

• Program is run with a team of nurses, counsellors, respiratory therapists, and a physician.

• Currently in 7 Addictions services located in community health centres in Vancouver.

Self-Harm And Suicidal Behaviors In Hong Kong Adolescents: Prevalence And Psychosocial Correlates, Daniel T. L. Shek, Lu Yu

Pediatrics Faculty Publications

The present paper examined the prevalence and psychosocial correlates of adolescent deliberate self-harm (DSH) and suicidal behavior in a representative sample of 3,328 secondary school students in Hong Kong. With reference to the previous year, 32.7% of the students reported at least one form of DSH, 13.7% of the respondents had suicide thoughts, 4.9% devised specific suicidal plans, and 4.7% had actually attempted suicide. Adolescent girls had significantly higher rates of DSH and suicidal behavior than did adolescent boys. Having remarried parents was related to an increased likelihood of DSH and suicide. While high levels of family functioning, overall positive …

Oral Microbial Biofilm Stimulation Of Epithelial Cell Responses, Rebecca Peyyala, Sreenatha S. Kirakodu, Karen F. Novak, Jeffrey L. Ebersole

Center for Oral Health Research Faculty Publications

Oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. However, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. This report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. Monoinfection biofilms were developed with Streptococcus sanguinis, Streptococcus oralis, Streptococcus gordonii, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis on rigid gas-permeable contact lenses. Biofilms, as well as planktonic cultures …

Global Ky (Spring 2012), University Of Kentucky International Center

Global Kentucky

No abstract provided.

Southeastern Law Librarian Spring 2012, Seaall

Newsletters

No abstract provided.

“Pico”: PRactice Ebm Skills, INcrease Student Interests With COllaboration Of Librarians And Improve OUtcomes, Archana Kudrimoti, Janice Kuperstein, Shari Levy, Tag Heister, Frank Davis

Library Presentations

Available literature on teaching evidence-based medicine (EBM) to medical students focuses on teaching critical appraisal skills, often in the context of a journal club, workshops or lectures. Being able to utilize EBM effectively means that a learner is able to take a clinical scenario, develop a clinically relevant question, search for the evidence, appraise that evidence, and apply the results of this appraisal back to the individual patient. Hence EBM activity is more likely to become a part of clinical decision-making if medical students practice the skills in the context of direct patient care.

A Non-Canonical E-Box Within The Myod Core Enhancer Is Necessary For Circadian Expression In Skeletal Muscle, Xiping Zhang, Samir P. Patel, John J. Mccarthy, Alexander G. Rabchevsky, David J. Goldhamer, Karyn A. Esser

Center for Muscle Biology Faculty Publications

The myogenic differentiation 1 (MyoD) gene is a master regulator of myogenesis. We previously reported that the expression of MyoD mRNA oscillates over 24 h in skeletal muscle and that the circadian clock transcription factors, BMAL1 (brain and muscle ARNT-like 1) and CLOCK (circadian locomotor output cycles kaput), were bound to the core enhancer (CE) of the MyoD gene in vivo. In this study, we provide in vivo and in vitro evidence that the CE is necessary for circadian expression of MyoD in adult muscle. Gel shift assays identified a conserved non-canonical E-box within the CE that …

Special Ops: Vulnerable Populations And Tobacco Treatment, Chizimuzo T.C. Okoli, Audrey Darville

Nursing Presentations

• Reasons for Smoking among individuals with co-morbid substance use (SUD)and/or psychiatric disorders (PD)

• Smoking cessation treatment for individuals with co-morbid substance use (SUD) and/or psychiatric disorders (PD)

• Smoking among the Homeless population

Drinking To Distraction: Does Alcohol Increase Attentional Bias In Adults With Adhd?, Walter Roberts, Mark T. Fillmore, Richard Milich

Psychology Faculty Publications

Previous research has shown that social drinkers continue to show attentional bias toward alcohol-related stimuli even after consuming a moderate dose of alcohol. In contrast, little is known about how alcohol acutely affects attentional bias in groups at risk to develop alcohol-related problems, such as adults with attentiondeficit/ hyperactivity disorder (ADHD). Such individuals may show increased attentional bias following alcohol relative to nonclinical controls. The present study tested this hypothesis by examining acute alcohol effects on attentional bias in 20 social drinkers with ADHD and 20 social drinkers with no history of ADHD. Participants performed a visual-probe task after receiving …

Evidence-Based Medicine Versus Personalized Medicine: Are They Enemies?, Jose De Leon

Psychiatry Faculty Publications

No abstract provided.

Evaluation Of The Training Program Of The Project P.A.T.H.S.: Findings Based On The Perspective Of The Participants From Different Cohorts, Daniel T. L. Shek, Yammy L. Y. Chak

Pediatrics Faculty Publications

Subjective outcome evaluation findings based on the perspective of the participants participating in a 3-day training program of the Project P.A.T.H.S. are reported in this paper. The findings were based on the data collected from the training workshops conducted between 2005 and 2009 (N = 4.167). Results showed that the respondents had good and positive perceptions of the training program and found it very valuable, particularly with respect to training instructors and familiarization with the project. Besides, the training participants were able to acquire attitude, knowledge and skills that are conducive to the successful implementation of the program. Based on …

Subjective Outcome Evaluation And Factors Related To Perceived Effectiveness Of The Project P.A.T.H.S. In Hong Kong, Daniel T. L. Shek, Lu Yu, Vicky Y. T. Ho

Pediatrics Faculty Publications

Based on a sample of 24,457 participated students, the present study investigated participants' subjective evaluation of the Tier 2 Program of the Project P.A.T.H.S. in the 2009/2010 academic year. Participants generally held positive views toward the Tier 2 Program and program instructor and perceived the program to be beneficial to their development. Programs involving adolescents alone were evaluated more positively than programs involving parents and/or teachers. Students' grade and program type did not show significant impact on participants' subjective evaluation of the project. Consistent with previous reports, perceived effectiveness of the program was significantly predicted by students' perceptions about the …

The Project P.A.T.H.S. In Hong Kong--Lessons Learned And Implications For Positive Youth Development Programs, Daniel T. L. Shek, Rachel C. F. Sun

Pediatrics Faculty Publications

The Project P.A.T.H.S. is a positive youth development program which attempts to promote holistic development of junior secondary schools in Hong Kong. It is ground breaking in terms of the number of schools participating in the project and financial resources injected into the project. Based on the experiences gained from the implementation of the project and evaluation data collected from 2005 to 2011, several issues pertinent to the development of positive youth development programs in the Chinese culture are discussed. These issues include complexity of program development, importance of training, identification of factors governing program implementation, need for evaluation, and …

Helping Adolescents With Greater Psychosocial Needs: Subjective Outcome Evaluation Based On Different Cohorts, Daniel T. L. Shek, Tak Yan Lee

Pediatrics Faculty Publications

The Tier 2 Program of the Project P.A.T.H.S. (Positive Adolescent Training through Holistic Social Programmes) is designed to help students with greater psychosocial needs. This paper examines nine sets of subjective outcome evaluation data collected from 2005 to 2009 (n = 60, 241 participants). Based on the consolidated data with schools as units, results showed that participants generally had positive perceptions of the program, implementers, and benefits of the program. The subjective outcome evaluation instrument was found to be internally consistent. Multiple regression analysis revealed that perceived qualities of the program and the program implementers predicted perceived effectiveness of the …

A Unique Treasure: Transylvania's 1,760 Medical Theses From The Early Nineteenth Century--11 On Yellow Fever, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

No abstract provided.

Sustainable Agriculture Undergraduate Degree Programs: A Land-Grant University Mission, Krista L. Jacobsen, Kim L. Niewolny, Michelle S. Schroeder-Moreno, Mark Van Horn, Alison H. Harmon, Yolanda H. Chen Fanslow, Mark A. Williams, Damian Parr

Horticulture Faculty Publications

There has been considerable growth in the number undergraduate degree programs in sustainable agriculture (SA) in universities and colleges across the country in the past 25 years. As a subset of this national trend, land-grant universities (LGUs) are emerging as catalysts in innovative SA program development, in part due to the LGU tripartite mission of education, extension, and research. This mission compels LGUs to develop undergraduate degree offerings to engage student, faculty, and community stakeholders who are increasingly interested in SA. In this article, which is an outcome of a gathering of faculty, staff and students from SA programs at …

Sustainable Agriculture Education And Civic Engagement: The Significance Of Community-University Partnerships In The New Agricultural Paradigm, Kim L. Niewolny, Julie M. Grossman, Carmen J. Byker, Jennifer L. Helms, Susan F. Clark, Julie A. Cotton, Krista L. Jacobsen

Horticulture Faculty Publications

Universities and colleges across the United States are making innovative strides in higher education programming to catalyze a more sustainable era of agriculture. This is clearly exemplified through the formation of community-university partnerships as critical illustrations of civic engagement (CE) for sustainable agriculture (SA) education. This paper explores the praxis of CE for SA education by focusing on the ways in which five land-grant universities (LGUs) with undergraduate programs in SA have developed and put into practice community-university partnerships. Drawing upon these programs and supportive literature, this article specifically attempts to describe the role and significance of CE for SA …

Chain Community Pharmacists' Willingness, Attitudes, And Barriers In Providing Self-Care Medication And Supplement Recommendations, Clark Kebodeaux, William M. Grise, Brooke Hudspeth, Holly Divine

Pharmacy Practice and Science Faculty Publications

BACKGROUND: Pharmacists are commonly called on as the first resource for patients when selecting an appropriate self-care medication or supplement. This study examines pharmacists’ over-the-counter (OTC) recommendations in the chain community pharmacy setting in the United States.

OBJECTIVES: The objective of the study was to assess chain community pharmacists’ willingness and attitudes to provide self-care recommendations to patients and to identify chain community pharmacists’ barriers to making OTC medication and supplement recommendations.

METHODS: An anonymous survey was created to identify pharmacists’ attitudes and their willingness to perform these recommendations. The questionnaire included specific questions to identify barriers that exist to …

Semi Tractor-Trailer Driver Dies In Median Crossover Crash; 10 Others Die, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Motor Vehicle

At approximately 5:00 AM on a spring morning, a 45 year-old male semi-tractor trailer driver headed south on an interstate highway with a back-haul of brake drums. He crossed a 60’ grass depressed median, drove over a cable barrier, was struck by a northbound 15-passenger van with 12 occupants, hit a cut rock wall and caught fire. The semi truck driver and 10 occupants within the van died.

To prevent future occurrences of similar incidents, the following recommendations have been made:

Recommendation No. 1: Commercial vehicle carriers should establish and implement an appropriate comprehensive safety and driver training program that …

Steel Worker Falls From Highway Bridge And Dies, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Falls

On a fall day in 2010, a 49 year-old steel worker fell from a highway bridge he was working from and died. The steel worker was in the process of leveling jacks for concrete forms. He had donned a fall protection harness with an attached lanyard then stepped outside the lifeline onto a 10”x2” wooden board to verify the jacks were level. As the steel worker stepped over the lifeline, he did not attach the lanyard and fell approximately 28 feet onto the railroad tracks below. He was taken to a nearby hospital where he died in surgery approximately two …

Does Emotional Memory Enhancement Assist The Memory-Impaired?, Lucas S. Broster, Lee X. Blonder, Yang Jiang

Behavioral Science Faculty Publications

We review recent work on emotional memory enhancement in older adults and patients with mild cognitive impairment (MCI) or Alzheimer dementia (AD) and evaluate the viability of incorporating emotional components into cognitive rehabilitation for these groups. First, we identify converging evidence regarding the effects of emotional valence on working memory in healthy aging. Second, we introduce work that suggests a more complex role for emotional memory enhancement in aging and identify a model capable of unifying disparate research findings. Third, we survey the neuroimaging literature for evidence of a special role for the amygdala in MCI and early AD in …

Inflammatory Responses To Induced Infectious Endometritis In Mares Resistant Or Susceptible To Persistent Endometritis, Mette Christoffersen, Elizabeth Woodward, Anders M. Bojesen, Stine Jacobsen, Morten R. Petersen, Mats H. T. Troedsson, Henrik Lehn-Jensen

Veterinary Science Faculty Publications

BACKGROUND: The objective of the study was to evaluate the gene expression of inflammatory cytokines (interleukin [IL]-1β, IL-6, IL-8, IL-10, tumor necrosis factor [TNF]-α, IL-1 receptor antagonist [ra] and serum amyloid A (SAA) in endometrial tissue and circulating leukocytes in response to uterine inoculation of 105 colony forming units (CFU) Escherichia coli in mares. Before inoculation, mares were classified as resistant or susceptible to persistent endometritis based on their uterine inflammatory response to infusion of 109 killed spermatozoa and histological assessment of the endometrial quality. Endometrial biopsies were obtained 3, 12, 24 and 72 hours (h) after bacterial inoculation and …

Oxidative Stress In Hpv-Driven Viral Carcinogenesis: Redox Proteomics Analysis Of Hpv-16 Dysplastic And Neoplastic Tissues, Federico De Marco, Elona Bucaj, Cesira Foppoli, Ada Fiorini, Carla Blarzino, Kozeta Filipi, Alessandra Giorgi, Maria Eugenia Schininà, Fabio Di Domenico, Raffaella Coccia, D. Allan Butterfield, Marzia Perluigi

Chemistry Faculty Publications

Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main etio-pathogenetic factor of cervical cancer, is not per se sufficient to induce tumour development. Oxidative stress (OS) represents an interesting and under-explored candidate as a promoting factor in HPV-initiated carcinogenesis. To gain insight into the role of OS in cervical cancer, HPV-16 positive tissues were collected from patients with invasive squamous cervical carcinoma, from patients with High Grade dysplastic HPV lesions and from patients with no clinical evidence of HPV lesions. After virological characterization, modulation of proteins involved in the redox status regulation was investigated. ERp57 and …

Motor Neuron Apoptosis And Neuromuscular Junction Perturbation Are Prominent Features In A Drosophila Model Of Fus-Mediated Als, Ruohan Xia, Yajuan Liu, Liuqing Yang, Jozsef Gal, Haining Zhu, Jianhang Jia

Markey Cancer Center Faculty Publications

BACKGROUND: Amyotrophic lateral sclerosis (ALS) is progressive neurodegenerative disease characterized by the loss of motor function. Several ALS genes have been identified as their mutations can lead to familial ALS, including the recently reported RNA-binding protein fused in sarcoma (Fus). However, it is not clear how mutations of Fus lead to motor neuron degeneration in ALS. In this study, we present a Drosophila model to examine the toxicity of Fus, its Drosophila orthologue Cabeza (Caz), and the ALS-related Fus mutants.

RESULTS: Our results show that the expression of wild-type Fus/Caz or FusR521G induced progressive toxicity in multiple tissues of the …