Digital Commons Network^™

Chronic Alcohol Consumption Induces Phenotypic And Functional Alterations Consistent With A Hyper-Inflammatory State In Peripheral Blood Mononuclear Cell-Derived Microglia In A Rhesus Macaque Model, Hami Hemati, Madison B. Blanton, Heather E. True, Jude Koura, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Alcohol-induced dysregulation of microglial activity is associated with neuroinflammation, cognitive decline, heightened risk for neurodegenerative diseases, alcohol dependence, and escalation of alcohol drinking. Given the challenge of longitudinally sampling primary microglia, we optimized an in vitro method to differentiate peripheral blood mononuclear cells (PBMC) from rhesus macaque (RM) into induced microglia-like cells (RM-iMGLs). The RM-iMGLs displayed transcriptional profiles distinct from monocyte progenitors and closely resembling primary microglia. Notably, morphological features showed that differentiated RM-iMGLs derived from subjects with chronic alcohol consumption (CAC), while bigger, exhibited a bipolar-like morphology. Additionally, dysregulation in key inflammatory and regulatory markers, along with increased baseline …

Adapting A Mobile Health Intervention To Improve Prep Adherence Among Young Black Msm In Fulton County, Georgia, Kassidy Maust

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Young Black men who have sex with men (YBMSM) in the southern United States, particularly in Fulton County, Georgia, face disproportionately high rates of HIV and unique structural barriers to pre-exposure prophylaxis (PrEP) uptake and adherence. Sustained adherence among YBMSM remains low due to intersecting structural, social, and behavioral barriers. To address these disparities, this paper presents an adapted implementation of the evidence-based M-Cubed (Mobile Messaging for Men) mHealth intervention. Prior studies of M-Cubed and similar mHealth interventions have shown promising outcomes in increasing PrEP adherence among Black MSM populations. The adapted intervention integrates culturally tailored messaging specific to behaviors …

Air Pollution Exposures And Cognitive Function Among Older Americans, Clarisse Nyondo Okoma

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Background: New findings indicate that environmental factors, such as air pollution, could contribute to cognitive decline among older adults. Nonetheless, extensive longitudinal studies in the U.S. investigating this connection are few and far between.

Objective: This study assessed the relationship between long-term exposure to ambient air pollutants and cognitive function in older adults in the United States.

Methods: We examined data from the Health and Retirement Study (2010-2018), which included 234,250 observations from adults aged 50 and older. Cognitive performance was evaluated based on immediate and delayed word recall, a 27-point cognitive summary score, backward from 20, and …

Implementing An Opioid Overdose Education And Naloxone Distribution (Oend) Program Among Law Enforcement Officers In Lee County, Ky, Keelie Jolly

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This grant proposal describes an implementation strategy for introducing an OEND training program among law enforcement officers (LEOs) to reduce opioid overdose deaths. The goal of the project is to increase LEOs' awareness and knowledge of opioid overdose and Naloxone administration and usage of Naloxone.

Rct Pre-Post Training Analysis Of The Vipcorps Maternal Mortality Prevention Course With A Focus On Intimate Partner Violence (Ipv), Rose A. Wood

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Maternal mortality rates rob families across the U.S. of a mother figure, and each death is preventable. The U.S. has the highest maternal mortality rate of high-earning countries. This study assesses the effects of the VIPCorps program, a course on the top 3 risk factors of maternal mortality, on improving knowledge and attitudes towards victims of IPV. Students in helping professions at the University of Kentucky (n = 223) were recruited via on campus advertising and remotely allocated to either the VIPCorps program or a mock maternal mortality training control. Generalized linear modeling was used to estimate the difference in …

Improving End-Of-Life Care: A Nursing Education Initiative, Spring C. Kingsley

DNP Projects

Background: This study addresses the gap in education and training for medical intensive care unit (MICU) nurses regarding the care of hospice patients. The traditional medical model of healthcare prioritizes illness treatment, often leading to inadequate end-of-life support and increased hospitalization during patients' final days. The rise of in-hospital hospice programs, or scatter-bed hospice models, highlights the need for nurses to enhance their skills in providing end-of-life compassionate care. The MICU at the University of Kentucky’s Chandler Hospital serves as a focal point for this initiative, given its role in caring for critically ill patients who require hospice attention.

Purpose: …

Demonstration Of An Enhanced Dosing Pattern For Debulking Large And Bulky Unresectable Tumors Via Differential Hole-Size Spatially Fractionated Radiotherapy, Joshua Misa, William St. Clair, Damodar Pokhrel

Radiation Medicine Faculty Publications

Purpose/objective

We propose a novel lattice deployment for spatially fractionated radiotherapy (SFRT) treatments. In this approach, a larger diameter high-dose sphere is centrally placed in the bulky tumor mass and surrounded by smaller diameter high-dose spheres.

Materials/methods

Thirty SFRT patients (10 head and neck [HN], 10 abdominal/pelvis, and 10 chest/lung cases) treated with an MLC-based crossfire method were retrospectively analyzed. Eleven differential hole-size lattice patterns were benchmarked against the clinically delivered SFRT plans (1 cm diameter cylinders, 2 cm spacing) and the standard uniform lattice pattern (1.5 cm diameter spheres, 3 cm spacing). These patterns varied in core diameter (C: …

In Vitro Α/Β Ratio Variations In Cervical Cancer, With Consequent Effects On Equivalent Dose In 2 Gy Fraction In High-Dose-Rate Brachytherapy, Cameron Thayer-Freeman, Brien Washington, Denise Fabian, Dennis C. Cheek, William St. Clair, Mark E. Bernard, Wei Luo

Radiation Medicine Faculty Publications

Purpose: To determine the distributions of published α/β values measured in vitro and the resulting uncertainties in the equivalent dose in 2 Gy fractions (EQD ₂ ) when applied to high-dose-rate brachytherapy (HDR-BT) for cervical cancer.

Methods and Materials: An analysis of 98 published α/β values from 31 papers was conducted, spanning 23 cervical cancer cell lines. Values were further divided into squamous cell carcinoma and adenocarcinoma histologies. Probability distributions were fit to histograms using the bootstrapped Kolmogorov-Smirnov goodness-of-fit test. Both average and most probable α/β values for cervical cancer were determined. The probability distributions were then applied to three …

Alternative Visions Of Swedish State Feminism, Lukas Bullock

Theses and Dissertations--Gender and Women's Studies

Sweden has been lifted as a prominent example of a successful feminist policy enactor, and has built credibility as a humanitarian actor both domestically and transnationally. Simultaneously, intersectionally marginalized groups have attested to patterns of social exclusion and feelings of political disenfranchisement in Swedish society, calling into question Sweden’s reputation for feminist exceptionalism. This dissertation project centers on unpacking this dynamic by examining how Swedish gender equality policymaking institutions design and implement feminist policy, and how those efforts are interpreted and contested by intersectionally marginalized groups of Afro-Swedish feminist activists. Drawing from a mixed-methods framework that includes feminist ethnography (interviews, …

Black Women, Bold Science: Navigating Race, Gender, And Identity In Science, Mia L. Brown

Theses and Dissertations--Science, Technology, Engineering, and Mathematics (STEM) Education

This research study examines the challenges Black women encounter when in predominantly white, male academic and professional science settings, where they experience dual discrimination because of their race and gender. These biases perpetuate the negative stereotype that Black women are less competent than white men. This mixed methods study examines how Black women navigate their race, gender, and science identities within predominantly white institutions through their doctoral programs and professional science careers. The study implements Critical Race Mixed Methodology to combine survey data for a wide reach with interview data for detailed exploration. The study involved eight Black women science …

The Role Of Cooperative Extension In Community Development: A Study Of Success Stories And Leadership Practices, George Grant

Theses and Dissertations--Educational Leadership Studies

The Cooperative Extension System (CES) plays a vital role in community education and development across the United States. CES agents and specialists work directly with community members to identify needs, mobilize existing assets, and empower action. Despite the overlap in the fields of CES and community development, few studies have systematically analyzed the specific practices used by extension personnel who act as facilitators of change within communities. To address this gap in knowledge, a qualitative study was conducted using directed content analysis of CES success stories published through the University of Kentucky to identify practices used among two integral positions …

A Survey Study Of Professional Competencies For Resident Directors: Perspectives From Resident Directors And Supervisors, Lauren Doerner

Theses and Dissertations--Educational Leadership Studies

Resident directors (RDs) play a critical role in the success of residence life programs on college campuses. However, there is limited research on the professional competencies necessary for success in this role. This study explores the relationship between the ACPA and NASPA professional competencies RDs and their supervisors identify as most critical for RD success. In this study, RDs and their supervisors ranked key competencies, assessed RDs' mastery of these competencies, and RDs identified how they acquired them. Using a survey research design, quantitative data were collected from RDs and their supervisors at Research I institutions in the southeastern United …

Navigating Stress In Higher Education Leadership: The Role Of Mindful Meditation For Women Administrators, Christine T. Harper

Theses and Dissertations--Educational Leadership Studies

Women higher education administrators encounter distinct stressors which can significantly impact wellbeing and lead to burnout. These challenges include implicit bias, difficulty achieving work-life balance, and increasing institutional expectations. This study utilizes a Mixed Methods Action Research (MMAR) framework to assess stress levels, trait mindfulness, coping mechanisms, and the effectiveness of a structured mindfulness-based intervention. While the quantitative analysis revealed only a slight increase in trait mindfulness and no notable reduction in stress levels, the qualitative findings showcased meaningful personal benefits to the participants. They reported heightened awareness of stress triggers, improved emotion regulation, and an ability to apply the …

International School Leaders’ Lived Experiences Of Crises And Perceptions Of Trust, Shary L. Marshall

Theses and Dissertations--Educational Leadership Studies

International school leaders worldwide have navigated their schools through a variety of crises. Their unique lived experiences offer insights into the role of trust in leadership, particularly during times of crisis.

This qualitative study employed a phenomenological approach to explore the lived experiences of 12 experienced international school leaders. Data was collected through semi-structured interviews. Using the theoretical lenses of authentic leadership, sensemaking, loose coupling, and crisis leadership, this study illuminates the central role of trust in leading schools through crises.

Three significant themes emerged from the analysis. First, trust is foundational to leadership and is built through intentional, transparent …

Path-Goal Leadership Dynamics In Advanced Manufacturing Dual Credit Career And Technical Education (Cte) Classrooms In Connecticut, Kyle R. Pepin

Theses and Dissertations--Educational Leadership Studies

This dissertation examined the adaptive instructional leadership practices of instructors teaching dual credit career and technical education (CTE) classes in Connecticut, particularly in advanced manufacturing technologies (AMT). The study drew on survey responses from a broader group of course instructors and a purposeful sample of six instructors and six adult students who participated in semi-structured interviews. All instructors taught dual credit classes in partnership with Connecticut State Community College in hands-on manufacturing, engineering, or similar workshop environments designed to prepare high school aged students for careers and develop practical, job-ready skills in settings that closely replicate industry conditions. This under-researched …

Innovative Biomechanical And Engineering-Based Assessments Of Hip Mechanics In Individuals With Hip-Related Pain, Holly M. Stanze

Theses and Dissertations--Kinesiology and Health Promotion

Individuals with hip-related pain exhibit altered movement patterns, and worse fear of movement (kinesiophobia), compared to asymptomatic controls. Exacerbation of hip pain during movement and the role of hip symptom duration may help further our understanding of specific movement patterns that are associated with hip-related pain. A better understanding of the potential connections between hip-related symptom duration, kinesiophobia, hip pain during movement, and lower limb mechanics in those with hip-related pain may allow for more efficient referrals to clinicians and lead to more effective rehabilitation. In addition, the impact of hip-related pain on intra-articular hip loading patterns is not well …

Psychological Impacts In Exercise Riders And Professional Jockeys Following Injury, Kelley M. Renner

Theses and Dissertations--Kinesiology and Health Promotion

One of the most well-known equestrian sports, horse racing, is not only a cornerstone of Kentucky’s economy, but also a vital part of its culture, with professional jockeys playing an essential role in the local communities and traditions that define the state. Despite its popularity and financial impact, mental health challenges within the sport have continually been shrouded in silence and ignored in research (Ball et al., 2007). Equally concerning is the high injury prevalence, and psychological impact of these injuries (e.g., psychological distress, depression, anxiety, loss of identity, and self-esteem (King et al., 2022; Losty et al., 2019; McConn-Palfreyman …

The Effects Habitual Training Shoe Use Has On Running Economy When Transitioning To High Performance Running Shoes, Dalton C. Harris

Theses and Dissertations--Kinesiology and Health Promotion

Background: The recent increase in popularity of minimal shoes in endurance running is linked to potential improvements in running economy and reduced injury risk. However, high performance running shoes, specifically designed to improve running economy remain the fastest shoe to wear when racing. Purpose: Knowing this, the aim of this paper is to examine whether or not the habitual use of minimal shoes increases the benefits of the high performance shoes. This will guide practitioners and athletes when establishing shoe rotations and training plans during race preparation. Methods: The study consisted of 4 sessions: Sessions 1 and 2 were maximal …

Student Spouses Of Retired Military Veterans: Exploring Their Journeys To Postsecondary Degrees, Elizabeth Austin

Theses and Dissertations--Educational Policy Studies and Evaluation

The purpose of this qualitative narrative study is to explore the experiences of spouses, specifically female spouses, of retired U. S. military veterans who pursued the completion of postsecondary degrees following the retirement of the military service member. The study employs narrative inquiry, guided by the Biographic Narrative Interview Method approach to interviewing. The generated data is analyzed utilizing thematic and narrative analysis methods, and a framework of two researchers’ sensitizing concepts is applied to understand and create meaning from the participants’ narratives. The participants’ narratives reveal a powerful truth about the impact of their lived experiences on their return …

Proximity And Density Of Tobacco Retail Locations To Kentucky Schools And The Relationship To Youth Perceptions Of Harm And Susceptibility To E-Cigarette Use, Hannah Cardona

Theses and Dissertations--Kinesiology and Health Promotion

Purpose: To explore the potential relationship between tobacco retailer proximity/density around Kentucky elementary, middle, and high schools, and students’ perceptions, perceived norms, susceptibility to, and use of e-cigarettes. Design: A mixed-methods analysis of youth’s self-reported survey results and geographical data. Setting: 106 Kentucky elementary, middle, and high schools which participated in #iCANendthetrend’s tobacco education program between September 2023 and May 2024. Subjects: Convenience sample of students from Ky schools (N = 7,823) Measures: Items assessing e-cigarette use, susceptibility, perceptions, and perceived norms. Analysis: ANOVA tests used to compare responses of elementary, middle, and high school students. Chi-squared tests were used …

Optimizing The Challenge: A Study On Promoting Intrinsic Motivation In Youth Physical Activity, Fan Zhang Dr.

Theses and Dissertations--Kinesiology and Health Promotion

This study investigates the intricate relationship between intrinsic motivation and various factors within the context of physical education (PE) among high school students. Drawing upon Self-Determination Theory as a conceptual framework, the study aims to unravel the influence of perceived challenge, perceptions towards physical activity (PA) during PE, personal characteristics, gender differences, PA levels, and their interactions on students' intrinsic motivation in PE.

A quantitative approach was adopted, utilizing surveys including the Challenge Scale, Intrinsic Motivation Inventory, Relatedness Scale, and Physical Activity Questionnaire for Adolescents. Data was collected from a convenience sample of 92 high school students from Great Crossing …

Exploring College Students’ Motivation For Physical Activity: Applications Of Self-Determination Theory And The Role Of Health And Wellness Education, Anne Russell Blevins

Theses and Dissertations--Kinesiology and Health Promotion

This dissertation includes three pieces of writing that aim to show the importance of physical activity for all ages but specifically college-aged students. The first piece of writing (Chapter 2) includes a teaching tip written for the Journal of Physical Education, Recreation, and Dance. Research has shown that dance was a subject of the past and many teachers did not feel comfortable teaching it. This practical piece was written to help show K-12 physical education teachers effective ways to involve students in physical activity specifically through teaching dance, mainly through using the “Teaching Dance for Understanding” model.

The second section …

You’Re Too Slow! Self-Selected Volitional Efforts Reveal Unexploited Neuromuscular Reserve Capacity Compared To Maximal Efforts During Fatiguing, Load Equated Resistance Exercise, Brian Benitez

Theses and Dissertations--Kinesiology and Health Promotion

This investigation examined how movement intent influences neuromuscular excitation strategies during fatiguing resistance exercise, comparing maximal intended concentric efforts versus self-selected volitional efforts during load-equated Smith machine back squat exercise to quantify reserve capacity and characterize underlying neuromuscular mechanisms. Twenty-three resistance-trained participants (12 males, 11 females) completed a repeated-measures design, with replication, across six visits, performing smith machine back squat repetitions-to-failure at 70% 1RM under maximal intent (accelerate rapidly) and volitional intent (self-selected effort) conditions. Maximal voluntary isometric contractions used rapid (instantaneous 10-second effort) and ramp (5-second build-up, 5-second maximal) protocols. Surface electromyography (sEMG) was recorded from vastus lateralis and …

Bridging Awareness And Access: Outdoor Recreation And Physical Activity In Eastern Kentucky, Timothy Mckay

Theses and Dissertations--Kinesiology and Health Promotion

Physical inactivity remains a pressing challenge in Appalachian Kentucky, where high obesity rates intersect with limited access to structured exercise facilities. This dissertation investigated the extent to which participation in outdoor recreation supports adherence to physical activity (PA) recommendations and explored community awareness of local public lands. Surveys from 52 adult participants in Knox County revealed strong engagement in outdoor activity, with participation significantly predicting compliance with national PA guidelines. Educational attainment was also associated with higher rates of PA compliance, pointing to the influence of literacy and awareness in health behaviors. Reliability testing of the Outdoor Physical Activity Attitude …

Magnetic Heating Efficiency Of Magnetite Nanoparticles: Dependence On Size And Alternating Magnetic Field, Sarah E. Kubican

Theses and Dissertations--Biomedical Engineering

Magnetic heating mediated by magnetic iron oxide nanoparticles (MION) has several clinical applications including cancer thermal therapy, tissue thawing for organ preservation, and magnetogenetics. However, current models of magnetic heating, including the widely used linear response theory, inadequately predict MION heating efficiency. An experimental study covering alternating magnetic field (AMF) strengths and frequencies ranging from 3.98 – 27.85 kA/m and 103.6 – 984.1 kHz, respectively, with a range of MION core diameters 9.5- 30.3 nm was conducted to elucidate the relationships between these AMF parameters and MION sizes and the resulting heating efficiencies. Heating efficiency was found to increase approximately …

Pathogen Surveillance Of Viruses And Antimicrobial Resistance Through Wastewater Based Epidemiology, William Strike

Theses and Dissertations--Biomedical Engineering

Pandemic surveillance is a cornerstone in public health safety, as it enables identification of pathogens circulating within a community before they become widespread outbreaks. Wastewater based epidemiology has blossomed in recent years due to its success in the SARS-CoV-2 pandemic, but this form of public health surveillance has a global equity problem. A large majority of the wastewater-based surveillance work has been done in large metropolitan cities with access to advanced testing infrastructure, which poses a challenge as we consider where pandemics have historically emerged. Therefore, there is a need to identify, adapt, and develop workflows to meet the needs …

Measuring Accessibility Of Electric Vehicle Charging Infrastructure: A Case Study In Kentucky, Oluwasola Elijah Fagbohun

Theses and Dissertations--Civil Engineering

This thesis assesses spatial accessibility to electric vehicle charging infrastructure across Kentucky by investigating methodological approaches to measure accessibility: provider-topopulation ratios, regional accessibility metrics, and several methods in the family of gravity models. The Enhanced Two-Step Floating Catchment Area method was employed to measure electric vehicle charging station accessibility across Kentucky using a network-based approach in ArcGIS Pro. The analysis integrated 2023 American Community Survey 5-year Census block group data, Kentucky road network datasets, charging station locations from the Alternative Fuels Data Center, and Alternative Fuel Corridor designations. We examined existing infrastructure and proposed stations outlined in the Kentucky electric …

A Scoring Approach For Evaluating And Ranking Complete Streets Projects, Eirini Stavropoulou

Theses and Dissertations--Civil Engineering

Complete Streets is a vital strategy for developing an equitable transportation network that provides access to all users and transportation options to communities. A growing interest in this concept has been noted in the United States, with over 1,700 Complete Streets-related policies implemented nationwide. The Kentucky Transportation Cabinet (KYTC) has developed the “Complete Streets, Roads, and Highways Manual” and the “Complete Streets Policy” aiming to promote a safer and more inclusive transportation system statewide through Complete Streets implementation. A recent KYTC initiative was the development of a scorecard to systematically evaluate Complete Streets projects competing for funding. The scorecard is …

Adjusting Benefit Estimation For Pedestrian And Bicycle Facilities For Kentucky, Dionysios Tzamakos

Theses and Dissertations--Civil Engineering

The growing shift of commuters towards active transportation (AT) in the U.S. has led transportation agencies to place greater emphasis on improving bicycle and pedestrian facilities. Such improvement efforts can be integrated into multi-modal highway planning and development or be implemented through stand-alone pedestrian and bicycle projects both resulting in additional AT demand. Measuring the benefits of infrastructure improvement is central to prioritizing projects, as active transportation contributes to health, congestion reduction, safety, and recreational benefits. This study aims to calibrate the demand estimates for walking and cycling and estimate the associated benefits for the state of Kentucky. Population density …

Advancing Vapor Intrusion Science Using Systematic Frameworks To Foster Informed Decision Making, Hong Cheng Tay

Theses and Dissertations--Civil Engineering

Vapor intrusion, the migration of subsurface vapors into indoor air spaces, is an important source of indoor air contamination to consider at hazardous waste sites contaminated by volatile organic compounds (VOCs). There is considerable uncertainty and variability in the factors that influence vapor intrusion processes, which poses a significant challenge for decision making, despite advancements in vapor intrusion science. This dissertation consolidates knowledge from existing scientific advances and investigates scientific gaps associated with the vapor intrusion preferential pathway conceptual model (i.e. conduit vapor intrusion), associated with the vapor entry pathway into buildings via sewer systems. The overall goal of this …