Digital Commons Network^™

A Twenty-First Century Approach To Darius Milhaud's Saudades Do Brasil, Paige H. Crain

Theses and Dissertations--Music

French composer Darius Milhaud (1892-1974) lived in Rio de Janeiro, Brazil from February 1917 to November 1918. Milhaud’s experience in Rio was overwhelmingly positive, and several of his works were influenced by this period of his life, one being a suite for solo piano titled Saudades do Brasil, Op. 67. Inspired by Brazilian popular music, this piece is an example of exoticism, a trend of writing music based on cultures other than the composer’s own. Considering that exoticism historically has the potential to perpetuate harmful stereotypes or generate false information, this research delves into Saudades do Brasil, exploring …

Professional Experiences Of Disabled Music Therapists: An Adapted Multiple Case Study, August Lily Tuggle

Theses and Dissertations--Music

Literature about disability often focuses on disabled people as recipients of professional services and there is scant literature about disabled professionals. Although music therapists work primarily with disabled individuals, there is little research on or from the perspectives of disabled music therapists. A growing body of qualitative research, grey literature, and media platforms provides perspectives from the disability community regarding music therapy. Disabled individuals both within and outside the profession critique common music therapy attitudes and practices that conflict with the expressed needs of the disability community. However, much of this literature contains the perspectives of one individual or a …

Analysis Of State And Local Requirements For Special Taxing District Fiduciary Managers And How Kentucky Could Improve, Zach Waller

MPA/MPP/MPFM Capstone Projects

This paper examines the oversight and training requirements for fiduciary managers within Special Purpose Government Entities (SPGEs), comparing practices in Kentucky with those in a select group of peer states. The analysis highlights notable deficiencies in fiduciary training, enforcement, and accountability standards in Kentucky, which lead to ongoing issues of financial mismanagement. A review of exemplary practices from other states highlights the importance of mandatory training, robust oversight frameworks, and effective enforcement measures in enhancing fiduciary outcomes. Recommended actions involve implementing mandatory fiduciary training programs, strengthening enforcement authority, and improving transparency mechanisms.

To evaluate Kentucky’s oversight of SPGEs compared to …

Beyond The Gas Tax: Evaluating Oregon’S Road Usage Charge (Orego) As A Model For Sustainable Infrastructure Funding, Gilles David Kwekam

MPA/MPP/MPFM Capstone Projects

Fuel tax revenues are declining nationwide due to electric vehicle adoption, improved fuel efficiency, and long periods without rate adjustments. This erosion has created long-term instability in transportation funding, including in Oregon, where revenue no longer aligns with road use. Road usage charges (RUCs) offer a potential replacement by charging drivers per mile traveled rather than per gallon consumed. However, their success depends less on technology and more on how programs are designed and governed.

This capstone examines which design features make RUC programs effective, using Oregon’s OReGO program as the central case. A Qualitative Comparative Analysis (QCA) comparing Oregon, …

Mind The Gap: Supporting Freshmen Voice Students Transitioning To Higher Education Through Coaching Conversations, Erica L. Gabriel

Theses and Dissertations--Music

The experiences one may encounter while matriculating through the K-12 system of education are vastly different from the world of higher education. While one feeds directly into the other, the transition can be a difficult one for young singers also navigating the developmental leap from adolescence to adulthood. This is due to several systematic and instructional differences, compounded with unique challenges encountered by freshman voice students in the first year. Gaps in support systems compiled with new and increasing financial, social, and academic demands can lead to a decline in overall student well-being and an increase in stress.

This increase …

Evaluating The Acceptability Of Ptsd Treatment Among Justice-Involved Women In Kentucky With Histories Of Opioid Use, Jesse P. Mccann

Theses and Dissertations--Psychology

Rates of trauma and PTSD as well as histories of opioid use are high among justice-involved women, particularly in Kentucky. However, there are many barriers to receiving treatment. There is a pressing need for brief, trauma-focused interventions that can be more feasibly delivered in jail or prison settings as well as by community clinicians serving women on probation or parole. This study explores the acceptability of treatment for PTSD, particularly Written Exposure Therapy (WET), among justice-involved women on probation or parole with a history of illicit or nonmedical prescription opioid use. The research aims to deepen our understanding of factors …

The Impact Of Victim Posttraumatic Stress Disorder (Ptsd) On Legal Decision-Making In A Rape Trial, Mary M. Levi

Theses and Dissertations--Psychology

Prior psychology and law research has investigated juror perceptions of victims and defendants with mental health issues. However, one factor that has yet to be fully examined empirically is how the presentation of a victim who developed a mental health disorder, such as posttraumatic stress disorder (PTSD), following a rape impacts legal decision-making. Understanding this impact is crucial considering over 36 million individuals in the United States report being a victim of rape in their lifetime, many of which report developing psychological disorders following the crime. Thus, the present study aimed to investigate how presenting mock jurors with victim testimony …

Unveiling Secrecy: A Qualitative Analysis Of The Breonna Taylor Grand Jury Proceedings, Taylor Bettis

Theses and Dissertations--Psychology

Grand juries have faced heightened scrutiny in recent decades following high-profile cases involving police use of lethal force against Black civilians. In 2020, Police officers killed Breonna Taylor while executing a search warrant at her home. Despite widespread public outcry, it remains unclear how prosecutors failed to indict the officers involved on charges directly related to her death. Guided by critical race theory, procedural justice, and theories of authority, the current project used reflexive thematic analysis to examine the Breonna Taylor grand jury proceedings. We identified two main themes: structural influences and narrative influences. Structural influences examine how prosecutorial authority, …

Methylome–Proteome Integration After Late-Life Voluntary Exercise Training Reveals Regulation And Target Information For Improved Skeletal Muscle Health, Toby L Chambers, Andrea Dimet-Wiley, Alexander R Keeble, Amin Haghani, Wen-Juo Lo, Gyumin Kang, Robert Brooke, Steve Horvath, Christopher S Fry, Stanley J Watowich, Yuan Wen, Kevin A Murach

Physiology Faculty Publications

Abstract: Exercise is a potent stimulus for combatting skeletal muscle ageing. To study the effects of exercise on muscle in a preclinical setting, we developed a combined endurance–resistance training stimulus for mice called progressive weighted wheel running (PoWeR). PoWeR improves molecular, biochemical, cellular and functional characteristics of skeletal muscle and promotes aspects of partial epigenetic reprogramming when performed late in life (22–24 months of age). In this investigation, we leveraged pan-mammalian DNA methylome arrays and tandem mass-spectrometry proteomics in skeletal muscle to provide detailed information on late-life PoWeR adaptations in female mice relative to age-matched sedentary controls (n = 7–10 …

Nonresolving Neuroinflammation Regulates Axon Regeneration In Chronic Spinal Cord Injury, Andrew N. Stewart, Christopher C. Bosse-Joseph, Reena Kumari, William M. Bailey, Kennedy A. Park, Victoria K. Slone, John C. Gensel

Physiology Faculty Publications

Chronic spinal cord injury (SCI) lesions retain increased densities of microglia and macrophages. In acute SCI, macrophages induce growth cone collapse and facilitate axon retraction away from lesion boundaries. Little is known about the role of sustained inflammation in chronic SCI or whether chronic inflammation affects regeneration. We used the colony-stimulating factor-1 receptor inhibitor, PLX-5622, to deplete microglia and macrophages months after complete crush SCI in female mice. Transcriptional analyses revealed a significant inflammatory depletion within chronic SCI lesions after PLX-5622 treatment. Both transcriptional analyses and immunohistochemistry revealed that Iba1⁺ cells repopulate to predepleted densities after treatment removal. Neuronal-enriched …

Programmable Scanning Diffuse Speckle Contrast Imaging Of Cerebral Blood Flow, Faezeh Akbari, Xuhui Liu, Fatemeh Hamedi, Mehrana Mohtasebi, Li Chen, Lei Chen, Guoqiang Yu

Physiology Faculty Publications

Significance: Cerebral blood flow (CBF) imaging is crucial for diagnosing cerebrovascular diseases. However, existing large neuroimaging techniques with high cost, low sampling rate, and poor mobility make them unsuitable for continuous and longitudinal CBF monitoring at the bedside. Aim: We aimed to develop a low-cost, portable, programmable scanning diffuse speckle contrast imaging (PS-DSCI) technology for fast, high-density, and depth-sensitive imaging of CBF in rodents. Approach: The PS-DSCI employed a programmable digital micromirror device (DMD) for remote line-shaped laser (785 nm) scanning on tissue surface and synchronized a 2D camera for capturing boundary diffuse laser speckle contrasts. New algorithms were developed …

Racial And Rural Disparities In Financial Toxicity And Healthcare Transitions Among Adolescent And Young Adult Cancer Survivors In Kentucky, Jean S. Edward, Brent J. Shelton, Lauren Corum, Haafsah Fariduddin, Katie Brown, John A. D’Orazio, Kimberly D. Northrip

Physiology Faculty Publications

Introduction: Adolescent and young adult cancer survivors, especially racial/ethnic minorities and rural residents are particularly vulnerable to financial toxicity due to limited healthcare access, socioeconomic disparities, and cultural/language barriers. These social determinants of health compound financial hardship and contribute to poor healthcare transitions from pediatric to adult care, leading to worse outcomes and higher mortality rates. Methods: Our cross-sectional survey study examined racial (Black vs White) and geographic (rural vs urban) disparities in financial toxicity and healthcare transition outcomes among 260 adolescent and young adult cancer survivors through the Kentucky Cancer Registry. Survey data were collected on financial toxicity, healthcare …

Differential Effects Of Myosin Activators On Myocardial Contractile Function In Nonfailing And Failing Human Hearts, Joohee Choi, Patrick T. Wood, Joshua B. Holmes, Katherine L. Dominic, Cristobal G. Dos Remedios, Kenneth S. Campbell, Julian E. Stelzer

Physiology Faculty Publications

The second-generation myosin activator danicamtiv (DN) has shown improved function compared with the first-generation myosin activator omecamtiv mecarbil (OM) in nonfailing myocardium by enhancing cardiac force generation but attenuating slowed relaxation. However, whether the functional improvement with DN compared with OM persists in remodeled failing myocardium remains unknown. Therefore, this study aimed to investigate the differential contractile responses to myosin activators in nonfailing and failing myocardium. Mechanical measurements were performed in detergent-skinned myocardium isolated from donor and failing human hearts. Steady-state force, stretch activation responses and loaded shortening velocity were analyzed at submaximal [Ca2+] in the absence or presence of …

Gastrocnemius Myofiber Type And Mitochondrial Alterations Associated With Peripheral Artery Disease Severity, Kate Kosmac, Rena Dana Wang, Jada Stewart, Parminder Kaur, Ahmed Ismaeel, Haseeb Ahsan, Lisa Hartnell, Esther E. Dupont-Versteegden, Mary M. Mcdermott, Robert L. Sufit, Luigi Ferrucci, Charlotte A. Peterson

Physiology Faculty Publications

The extent of walking impairment varies among individuals with peripheral artery disease (PAD), which may reflect differences in the adaptability of lower extremity muscles to ischemia-reperfusion injury characteristic of the disease. Analyses of gastrocnemius muscle biopsies from 113 individuals with PAD [mean ankle-brachial index (ABI) = 0.65 ± 0.13, 38 (33.6%) women, 76 (67.2%) Black] showed a wide range of myofiber type distributions (9.6%-82.6% type 1 myofibers). The abundance of oxidative type 1 myofibers negatively correlated with ABI (r = −0.22, P = 0.02), a measure of PAD severity. The abundance of type 1 myofibers also negatively correlated to 2a/x …

Neuro293, Joshua T. Moses, Fahad B. Shah, Nicholas M. Mcvay, Dylan E. Capes, Christopher C. Bosse-Joseph, Jocelyn Salazar, Victoria K. Slone, John E. Eberth, Jonathan Satin, Andrew N. Stewart

Physiology Faculty Publications

Efficient interrogation of neurobiology remains bottlenecked by obtaining mature neurons. Immortalized cell lines still require lengthy differentiation periods to obtain neuron-like cells, which may not efficiently differentiate and are challenging to transfect with plasmids relative to other cell lines such as HEK-293's. To overcome challenges with limited access to cells that express mature neuronal proteins, we knocked out the RE1-silencing transcription factor (REST) from HEK-293's to create a novel neuron-like cell, which we name Neuro293. RNA-sequencing and bioinformatics analyses revealed a significant upregulation of genes associated with neurobiology and membrane excitability including pre-/post-synaptic proteins, voltage gated ion channels, neuron-cytoskeleton, as …

Skeletal Muscle Stem Cell Mitochondria Are Transferred To Muscle Fibers In Response To A Hypertrophic Stimulus, Jensen Goh, Julia G. Williams, Sarah E. Ogle, Jai K. Joshi, Logan N. Scott, Benjamin Burke, Alex R. Keeble, Nicholas T. Thomas, Christopher S. Fry, Ahmed Ismaeel, John J. Mccarthy

Physiology Faculty Publications

The fusion of skeletal muscle stem cell (MuSC) to myofibers during hypertrophy has exclusively focused on the transfer of the MuSC nucleus, leaving the fate of other MuSC organelles, such as mitochondria, largely unexplored. The objective of this study was to determine whether MuSCs transfer their mitochondria upon myofiber fusion in response to a hypertrophic stimulus. To achieve this goal, we specifically labeled MuSC mitochondria with Dendra2 fluorescence by crossing the MuSC-specific CreER (Pax7^CreER/CreER) mouse with the Rosa26-Dendra2 mouse to generate the Pax7-Dendra2 mouse. To induce the fusion of MuSC to myofibers, Pax7-Dendra2 mice underwent synergist ablation surgery …

A Unionid Mussel Biodiversity Hotspot Experiencing Unexplained Declines: Evaluating The Influence Of Chemical Stressors Using Caged Juveniles, W. Aaron Wilson, Christine Bergeron, Jennifer Archambault, Jason M. Unrine, Jess Jones, Braven Beaty, Damian Shea, Peter R. Lazaro, Jody L. Callihan, Jennifer J. Rogers

Plant and Soil Sciences Faculty Publications

Unionid mussel populations in a section of the Clinch River in Virginia, USA, has declined substantially, but the causes of the decline remain unknown. To investigate this zone of decline (ZOD), we deployed juvenile freshwater mussels (Villosa iris in 2012 and Lampsilis fasciola in 2013) in both cages and silos at sites within the Clinch River System. We analyzed mussel tissues for trace element and organic contaminant concentrations, shells for trace elements, and environmental media (total water, dissolved water, particulate sediment, and bedload sediment) for both inorganic and organic contaminants. We found a few differences between mussels deployed in cages …

Breaking Ground On Self-Efficacy: A Qualitative Exploration Of Intimate Partner Violence Survivors’ Experiences With Therapeutic Horticulture, Rachel Marie Reeves

Theses and Dissertations--Sociology

This dissertation explores how participation in a Therapeutic Horticulture program may support the self-efficacy of survivors of Intimate Partner Violence. Through a thematic analysis of interviews with 45 women living at a domestic violence shelter in Lexington, Kentucky, this study investigates whether and how gardening activities contributed to participants’ belief in their abilities to shape outcomes in their lives.

Findings are organized around Bandura’s four sources of self-efficacy: enactive mastery experiences, vicarious experience, verbal persuasion, and physiological/affective states. These insights contribute to feminist criminology and trauma-informed care literature by illuminating the transformative potential of nature-assisted healing programs.

Emplacing Deaths Of Despair Within The Rural Mortality Penalty: The Spatial Inequality Of Violent Death Across Rural America, Cameron A. Mcalister

Theses and Dissertations--Sociology

American life expectancy continues to diverge from most developed nations. Literature spanning several fields of scholarship has documented an uneven distribution of life expectancy between social groups and across geographic contexts. Frameworks such as “Deaths of Despair” and the rural mortality penalty describe the declining health trends among working class whites and rural America. But, conceptual development around these frameworks is still limited. This dissertation utilizes a rural sociological approach to bridging the gap between the Deaths of Despair framework and the rural mortality penalty by examining violent death patterns across diverse rural contexts. In doing so, I incorporate several …

Temporal Dynamics Of Programmed Genome Rearrangement In Sea Lamprey Embryogenesis: A Deep Learning & Lightsheet Imaging Approach To Studying Early Developmental Transitions, Kasturi Rajandran

Theses and Dissertations--Biology

Programmed genome rearrangement (PGR) in the sea lamprey (Petromyzon marinus) leads to the elimination of ~0.5Gb (20%) of the germline genome during early embryogenesis, yet its timing relative to the maternal-to-zygotic transition (MZT) remains unclear. In this study, we imaged embryos cleared at 3-hour intervals (up to 72 hours post fertilization (hpf)) using high-resolution lightsheet fluorescence microscopy and a custom deep-learning image-analysis pipeline (~80% accuracy) to quantify nuclei and micronuclei counts. Micronuclei, which are the hallmarks of PGR first appear between 24 to 27hpf (vs ~36hpf previously reported) indicating PGR starts earlier than previously reported in the sea …

Engaging Nurses In Effective Cost Of Care Conversations To Address Cancer-Related Financial Toxicity: Results From An Exploratory Survey, Jean Edward, Amanda T. Wiggins, Louis G. Baser, Haafsah Fariduddin, Joanna F. Doran, Monica F. Bryant, John August D'Orazio, Kimberly D. Northrip

Markey Cancer Center Faculty Publications

Few evidence-based trainings exist on how to equip healthcare providers, particularly nurses, with the skills to engage in cost of care conversations with patients/caregivers to mitigate the impact of cancer-related financial toxicity. This study evaluated a pilot training developed in collaboration with Triage Cancer® to prepare oncology nurses to identify and assist patients/caregivers facing financial and/or legal barriers to care. Ten pediatric oncology nurses completed the training and pre/post-surveys on behaviors related to financial and legal need screening, frequency and comfort level of answering questions, knowledge, and behavior changes, along with training evaluation questions. At baseline, six nurses reported never …

Urobilin Derived From Bilirubin Bioconversion Binds Albumin And May Interfere With Bilirubin Interacting With Albumin: Implications For Disease Pathology, Kevin I. Williams, Priyanka Suryadevara, Chang-Guo Zhan, Terry D. Hinds Jr., Zachary A. Kipp

Markey Cancer Center Faculty Publications

Background/Objectives: Bilirubin is a hydrophobic molecule that binds the carrier protein albumin for transport through systemic circulation. Bilirubin is cleared from the body through the liver and excreted into the intestines, where the microbiota modifies the chemical structure, forming urobilin, which can be reabsorbed into circulation by the hepatic portal vein. Urobilin has no known function. It is also unknown whether urobilin binds albumin for transport in circulation. We hypothesized that because of the likeness of their chemical structures, urobilin would also bind albumin like bilirubin does. Methods: First, we used in silico docking to predict if urobilin would bind …

Dissecting The Biophysical Mechanisms Of Oleate Hydratase Association With Membranes, William A. Lathram, Robert J. Neff, Ashley N. Zalla, James D. Brien, Vivekanandan Subramanian, Christopher D. Radka

Markey Cancer Center Faculty Publications

This study investigates the dynamics of oleate hydratase (OhyA), a bacterial flavoenzyme from Staphylococcus aureus, and its interactions with lipid membranes, focusing on the factors influencing membrane binding and oligomerization. OhyA catalyzes the hydration of unsaturated fatty acids, playing a key role in bacterial pathogenesis by neutralizing host antimicrobial fatty acids. OhyA binds the membrane bilayer to access membrane-embedded substrates for catalysis, and structural studies have revealed that OhyA forms oligomers on membrane surfaces, stabilized by both protein-protein and protein-lipid interactions. Using fluorescence correlation spectroscopy (FCS), we examined the effects of membrane curvature and lipid availability on OhyA binding to …

Engaging Nurses In Effective Cost Of Care Conversations To Address Cancer-Related Financial Toxicity: Results From An Exploratory Survey, Jean Edward, Abigail Wiggins, Louis G. Baser, Haafsah Fariduddin, Joanna F. Doran, Monica F. Bryant, John A. D'Orazio, Kimberly D. Northrip

Markey Cancer Center Faculty Publications

Few evidence-based trainings exist on how to equip healthcare providers, particularly nurses, with the skills to engage in cost of care conversations with patients/caregivers to mitigate the impact of cancer-related financial toxicity. This study evaluated a pilot training developed in collaboration with Triage Cancer® to prepare oncology nurses to identify and assist patients/caregivers facing financial and/or legal barriers to care. Ten pediatric oncology nurses completed the training and pre/post-surveys on behaviors related to financial and legal need screening, frequency and comfort level of answering questions, knowledge, and behavior changes, along with training evaluation questions. At baseline, six nurses reported never …