Digital Commons Network^™

Development Of Tools For Atom-Level Interpretation Of Stable Isotope-Resolved Metabolomics Datasets, Huan Jin

Theses and Dissertations--Toxicology and Cancer Biology

Metabolomics is the global study of small molecules in living systems under a given state, merging as a new ‘omics’ study in systems biology. It has shown great promise in elucidating biological mechanism in various areas. Many diseases, especially cancers, are closely linked to reprogrammed metabolism. As the end point of biological processes, metabolic profiles are more representative of the biological phenotype compared to genomic or proteomic profiles. Therefore, characterizing metabolic phenotype of various diseases will help clarify the metabolic mechanisms and promote the development of novel and effective treatment strategies.

Advances in analytical technologies such as nuclear magnetic resonance …

Effects Of Sleep Fragmentation On The Progression Of Alzheimer’S Disease, Lindsay Beechem

Lewis Honors College Capstone Collection

Alzheimer’s Disease (AD) is a neurodegenerative disease that is characterized by amyloid-beta (Aβ) plaques, neurofibrillary tangles, neuronal death, and profound cognitive impairment. Previous studies have indicated that increased Aβ and alterations in the daily sleep-wake cycle are early risk factors and possible predictors of AD. Acute sleep deprivation decreases Aβ clearance, and increased Aβ levels stimulate neuroinflammation and accelerate loss of neurons and synapses. Likewise, it has been shown that there are higher rates of sleep disorders in AD patients. However, limited studies have investigated whether sleep fragmentation accelerates the progression of AD pathology. This partial review will discuss experiments …

An Exploration Of Sleep Fragmentation And Sleep Enhancement In Mice, Frannie Salisbury

Lewis Honors College Capstone Collection

Alzheimer’s Disease (AD) is a neurodegenerative disease that is can be defined by amyloid beta (Aβ) plaques, neurofibrillary tangles, and neuronal death. AD patients have characteristically fragmented sleep, with frequent nighttime awakenings and daytime naps. Decreased clearance of Aβ from disrupted sleep may be a contributing factor in the progression of AD. This study investigated how sleep fragmentation effected the progression of AD in 3xTgAD mice. Results showed that sleep fragmented mice had significantly more Aβ build-up in the hippocampus and thus greater progression of AD when compared to control mice. Improving sleep may be one way to reduce the …

Cross Approximation Methods For Integral Equation Matrices With Complex Structure, Jordon N. Blackburn

Theses and Dissertations--Electrical and Computer Engineering

Electrical and computer engineers rely on electromagnetic field (EM) theory to formulate and design systems that utilize information or energy obtained from a signal. Over time these systems have been increased in scale and complexity and adapted to handle a wider array of problems. This has motivated substantial developments in computational sciences including the area of computational electromagnetics (CEM).The focus of CEM is the simulation of electromagnetic fields. At the University of Kentucky, the CEM group has developed several modeling tools that are based on the application of approximation theory to integral equations. This allows the physical problem to be …

Negative Regulatory Factors In Tombusvirus Replication: Known Proteins, Novel Roles, Paulina Alatriste González

Theses and Dissertations--Plant Pathology

Although host cells are a rather rich source for co-opted host factors, lipids and metabolites, positive stranded RNA viruses vastly rewire cellular pathways and remodel cellular membranes to support viral replication. To accomplish such major changes, these viruses depend on the availability of different host factors and the ability to readily assemble viral replication organelles (VROs). Genome-wide screens and proteomics approaches with Tomato Bushy Stunt Virus (TBSV) in a yeast model host indicated that tombusviruses rely on the cellular cytoskeleton to reorganize the cellular environment of their hosts. Using temperature-sensitive (ts) mutants of beta and gamma-tubulin proteins and pharmacological inhibitors, …

Machine Learning And Bioinformatic Insights Into Key Enzymes For A Bio-Based Circular Economy, Japheth E. Gado

Theses and Dissertations--Chemical and Materials Engineering

The world is presently faced with a sustainability crisis; it is becoming increasingly difficult to meet the energy and material needs of a growing global population without depleting and polluting our planet. Greenhouse gases released from the continuous combustion of fossil fuels engender accelerated climate change, and plastic waste accumulates in the environment. There is need for a circular economy, where energy and materials are renewably derived from waste items, rather than by consuming limited resources. Deconstruction of the recalcitrant linkages in natural and synthetic polymers is crucial for a circular economy, as deconstructed monomers can be used to manufacture …

The Effect Of Oil Type, Oil Quality, Vitamin E, And Phytase Supplementation On Broiler Performance, Apparent Nutrient And Energy Utilization, And The Fatty Acid Profile Of Fat And Liver In Broiler Chickens, Richard A. Adefioye

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted to examine the effects of oil type, oil quality, phytase, and vitamin E (VE) supplementation in broiler chickens. Experiment 1 used 378-day-old male by-product Cobb breeder chicks with 9 treatments, 7 replicates, and 6 birds per replicate, structured as a 2x2x2+1 factorial arrangement of treatments for 14 days. The first 8 treatments were based on a marginally non-phytate phosphorus (NPP) deficient diet (0.31%), while treatment 9 was a positive control (PC) diet with adequate NPP levels (0.45%). There were 2 levels of phytase (0 vs 1000 FTU/kg), 2 levels of oil quality (fresh soy oil; peroxide …

Impact Of Preferential Flow, Source Water Connectivity, And Agricultural Management Practices On Sediment And Particulate Phosphorus Dynamics In Midwestern Tile-Drained Landscapes, Saeid Nazari

Theses and Dissertations--Biosystems and Agricultural Engineering

Tile drainage is recognized as a significant transporter of sediment and particulate phosphorus (PP) in the Midwestern U.S., leading to proliferation of Harmful Algal Blooms (HABs). Numerous studies have focused on Dissolved Reactive Phosphorus (DRP) and Nitrogen (N) flux dynamics in tile-drained landscapes; however, the impact of preferential flow and agricultural management practices on fate and transport of sediment and PP has remained poorly understood. The overarching objective of this study was to improve understanding of sediment P delivery in tile-drained landscapes. This dissertation focuses on four studies. In the first study, forms and flow pathway dynamics of total phosphorus …

Investigations Into The Genetics Of Mixed Pathologies In Dementia, Adam Dugan

Theses and Dissertations--Epidemiology and Biostatistics

Alzheimer’s disease (AD) is an irreversible, progressive brain disorder that leads to a loss of memory and thinking skills. While tremendous progress has been made in our understanding of the genetics underlying AD, currently known genetic variants explain only approximately 30% of the heritable risk of developing AD. One hurdle to AD research is that it can only be definitively diagnosed at autopsy, making cruder, clinic-based diagnoses more common. In recent years, several brain pathologies that mimic AD’s clinical presentation have been identified including brain arteriolosclerosis, hippocampal sclerosis (HS), and, most recently, limbic-predominant age-related TDP-43 encephalopathy (LATE). It has become …

An Assessment Of Kentucky Birth Records, Focusing On Early-Onset Hypertensive Disorders Of Pregnancy, Environmental Metal Exposures, And Geocoding Precision, 2008-2017, Courtney J. Walker

Theses and Dissertations--Epidemiology and Biostatistics

Using live and stillbirth records from Kentucky (2008-2017), this dissertation assessed the county-level prevalence and geospatial patterns of early-onset hypertensive disorders of pregnancy (eHDP); examined the geocoding precision of addresses recorded on birth records, and evaluated the association between individual risk factors and environmental metal exposures on eHDP prevalence. After adjusting for maternal demographic factors and pre-existing health conditions, we observed that eHDP prevalence was 38% higher (aPR=1.38, 95%CI:1.16, 1.64) in counties with the highest prevalence of married women (> 53.8%) compared to lower prevalence areas (31.6%) had a 20% higher prevalence of eHDP(aPR=1.20, 95%CI:1.00, 1.44) compared to counties with …

Scalable Approaches For Auditing The Completeness Of Biomedical Ontologies, Fengbo Zheng

Theses and Dissertations--Computer Science

An ontology provides a formalized representation of knowledge within a domain. In biomedicine, ontologies have been widely used in modern biomedical applications to enable semantic interoperability and facilitate data exchange. Given the important roles that biomedical ontologies play, quality issues such as incompleteness, if not addressed, can affect the quality of downstream ontology-driven applications. However, biomedical ontologies often have large sizes and complex structures. Thus, it is infeasible to uncover potential quality issues through manual effort. In this dissertation, we introduce automated and scalable approaches for auditing the completeness of biomedical ontologies. We mainly focus on two incompleteness issues -- …

Geophysical Techniques Used For The Analysis And Prediction Of Soil Strength And Mechanical Behavior, Matthew A. Hurley

Theses and Dissertations--Civil Engineering

Determining soil properties involves extensive laboratory testing of samples at discrete timing and location. Non-destructive analysis methods, such as electrical and seismic, presents alternatives to means of gathering soil properties accompanied with increased flexibility due to spatial and temporal applications. This research examined the ability of seismic wave data to predict soil behavior such as stress-strain and pore pressures using a modified version of Duncan and Chang (1970). Friction angle predictions were also analyzed using shear wave velocity and a modified form of the Santos and Gomez-Correria (2001) equation. This research also analyzed the use of electrical data to predict …

Context Classification Of Roadways Using Nationally Available Gis Data, Laura Wright

Theses and Dissertations--Civil Engineering

A Context Classification System based on context attributes of the area surrounding a roadway provides detailed information about the environment of a roadway corridor, while enabling practitioners to cater roadway projects to the community they are within. This study sought to create a system that would automatically classify roadway segments into the correct context, using data sources that are available nationwide. The proposed approach was tested by classifying the roadways in Highway District 7 of the Kentucky Transportation Cabinet. This process would make context classification approachable to more organizations, as it would reduce the amount of time required to apply …

Using Semialgebraic Parametric Analysis By Metaprogramming In Portfolio Optimization, Philip Blaine Meersman

Lewis Honors College Capstone Collection

One classic problem in quantitative finance is portfolio optimization, which consists of assigning weights to assets in a portfolio to maximize one’s expected return while keeping the level of risk at a desired level. This problem can be modeled as a linear program (LP), using a risk aversion parameter mu. For a given single value of mu, the LP can be solved using any standard LP solver. In this work, however, the problem is considered parametrically: the optimal solution is sought for every possible value of mu. This describes how weights to the portfolio assets would be assigned from the …

Best Practices For Research Analytics & Business Intelligence Within The Research Domain, Baron Wolf, Terri Hall, Katherine Robershaw

Research Analytics Academic Publications

While an increased number of industries with business operations have been shaping their use of data analytics, the use of such tools and methods within the higher education research domain, specifically research administration, is still in its infancy. This mixed-methods study collected data to identify best practices in how universities and other research organizations use data analytics to drive their strategic agendas, create efficiency in operations, and promote complex research proposals throughout their institutions. Research methods included a survey to collect data on how research offices are using analytics and business intelligence tools, Rasch analysis (Rasch, 1993) to examine survey …

Three Essays On Price Analysis And International Trade, Wei Zhang

Theses and Dissertations--Agricultural Economics

The first chapter of this dissertation examines price discovery and volatility spillovers in the coffee spot-futures commodity market over the post-ICA period by employing the PT-IS and bivariate EGARCH models. This research covers two actively traded coffee types, Arabica and Robusta, and utilizes daily time-series price data over 1990:01-2020:04 for Arabica and over 2008:01-2020:04 for Robusta. The empirical results indicate that coffee spot markets play a dominant role in the coffee price discovery for both Arabica and Robusta. The results also show volatility spillovers take place from the coffee spot market to the futures markets. This study helps small-scale coffee …

Productivity And Efficiency Difference Among Kentucky Grain Farms, Ahmed Yahya Hussein

Theses and Dissertations--Agricultural Economics

This paper attempts to estimate productivity and efficiency for Kentucky grain farms by applying a two-stage Date Envelopment Analysis (DEA) and DEA-based Malmquist method. The study covers the years 1999-2015. Also, productivity and efficiency testing hypotheses among different farm sizes and years are estimated. In the first step, productivity and efficiency indices are estimated through deterministic DEA. In the second stage, a panel regression is run with exogenous variables to explain the productivity and efficiency variation. In general small farms were found to be the least scale efficient compared to mid-sized and large farms, even though the results show overall …

Work-Related Changes In The Trunk Stiffness Of Nursing Personnel, Clare Tyler

Theses and Dissertations--Biomedical Engineering

Low back pain (LBP) is a significant issue related to spinal stability and, therefore, to trunk stiffness. Due to the nature of their work, nursing personnel are exposed to potential risk factors for LBP, such as lifting and trunk flexion, which have been reported in the literature to lead to decreases in trunk stiffness. Consequently, the purpose of this study was to investigate potential occupational effects on the trunk stiffness in nursing personnel. Twenty-four nursing personnel participated in this study and completed two sessions (pre-shift and post-shift) during which two passive flexion tests (with and without an ~7.5-lb load) were …

Adjudicating On-The-Track Outcomes In Kentucky: Why Horse Administrators Should Provide Competitors A Right To Review, Howard Ball

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

A Within-Session Analysis Of The Interview Informed Synthesized Contingency Analysis In A University Clinic Setting, Jenna M. Venuto

Theses and Dissertations--Early Childhood, Special Education, and Counselor Education

In this study, a within-session analysis of the interview informed synthesized contingency analysis (IISCA) was conducted to determine if the function of challenging behavior could be determined within the first 5 min test session and the extent that results from the first 5 min test session aligned with results from the full IISCA. An alternating treatment design was used to evaluate differentiated rates of challenging behavior during a full IISCA and the within-session analysis for four clients in a clinic setting. Results indicated that contingencies maintaining challenging behavior could be identified within the first 5 min test session and align …

Rate To Measure Mathematics Teaching: Using The Many-Facet Rasch Modeling To Reevaluate The Mathematics Classroom Observation Protocol For Practices (Mcop2), Chunling Niu

Theses and Dissertations--Education Sciences

Rater-mediated classroom observation protocols are increasingly being used for teaching performance assessments, which makes identifying and controlling for various rater effects a central issue to ensure the rating quality. A series of validation studies under the classical test theory framework, including content validity, interrater reliability, and structure analysis, have been completed for the 16-item Mathematics Classroom Observation Protocol for Practices (MCOP²).

However, the MCOP² data have never been investigated under the Rasch framework. Due to the methodological limitations of the CTT approach for rater-mediated assessments, it is imperative to examine the MCOP² validity and reliability using …

The Potential Role Of Trauma-Informed Positive Education In The Secondary Mathematics Classroom In Disrupting The Preschool-To-Prison Pipeline, Jessica Michele Doering

Theses and Dissertations--Education Sciences

This mixed-methods study considered the potential for trauma-informed mathematics education to disrupt the preschool-to-prison (or school-to-prison) pipeline. Phenomenological qualitative interviews were conducted in conjunction with the use of the Attitudes Related to Trauma-Informed Care (ARTIC; Baker et al., 2016) scale to determine teacher perceptions of trauma-informed care practices, their thoughts regarding challenging classroom behaviors and the connection of these behaviors with trauma and the pipeline, and their ideas about how much of an impact teachers can have on students who present with challenging behaviors that might be symptoms of trauma or that might be an indicator of future incarceration. This …

Sediment Transport Investigation In A Karst Basin Defines Governing Transport Processes And Explains Hysteresis Patterns, Leonie Bettel

Theses and Dissertations--Civil Engineering

Water resources researchers have advanced our understanding of sediment transport in karst aquifers and cave systems in recent years. However, we find knowledge gaps for sediment transport in karst including knowledge of processes controlling sediment hysteresis patterns from sensor datasets, and knowledge of dominant sediment transport processes that lead to sediment transport formulas for prediction. To address these gaps, we analyze a new suspended sediment, electrical conductivity and water flowrate datasets from sinking streams and a phreatic cave system for an epigenetic karst basin in the inner bluegrass region of Kentucky USA.

Three sediment patterns are common including: pulses of …

Three Essays On Export Earnings Volatility, Yoonseon Han

Theses and Dissertations--Economics

My dissertation consists of three essays on export earnings volatility. In the first essay, I look at the determinants of export earnings volatility. The four main determinants suggested by the theoretical literature are export concentration, durability, vertical linkages, and financial vulnerability. A panel of bilateral trade data at the industry level, containing 178 exporting countries, 194 importing countries, and 590 SITC Rev. 1 four-digit industries from 1962 to 2011, is used and these aspects are incorporated to empirically examine their effects on export earnings volatility. The essay finds that diversifying exports across different industries and trade partners plays an important …

Essays On The Role Of Policies In Major Public Health Issues, Anh Le

Theses and Dissertations--Economics

This dissertation explores the role of policy on health outcomes and behaviors that relate to major public health concerns. Essay 1 and Essay 2 investigate the effects of Medicaid expansions on substance-use outcomes. Essay 3 examines the impacts of school reopenings in Texas on COVID-19 and mobility outcomes.

Essay 1 studies the effect of the Affordable Care Act Medicaid expansion. Using State Drug Utilization Data 2011-2017, I find that the Medicaid expansion is associated with an increase of 40-60 Medicaid-paid opioid prescriptions per 1,000 people aged 19–64. However, the results suggest that post-expansion prescriptions are, on average, shorter or prescribed …

How Does Occupational Licensing Affect Entry Into The Medical Field? An Examination Of Emts, Aaron Yelowitz, Samuel J. Ingram

Institute for the Study of Free Enterprise Working Papers

The COVID-19 pandemic has led to temporary suspensions of many occupational licensing laws, especially for health care professionals, in an effort to manage surges in health care demand. The crisis highlights more general concerns about occupational licensing laws, yet convincing empirical evidence on the degree to which such laws have inhibited entry into health care professions is scarce because most studies must rely on cross-sectional variation to identify such effects. In this study, we indirectly examine how occupational licensing affects the choice to become an Emergency Medical Technician (EMT) by exploiting the demand-side shock from the Affordable Care Act (ACA). …

Targeting Intensive Job Assistance To Ex-Offenders By The Nature Of Offense: Results From A Randomized Control Trial, Christopher R. Bollinger, Aaron Yelowitz

Institute for the Study of Free Enterprise Working Papers

As many as two-thirds of newly-released inmates will be arrested for a new offense within three years. This study evaluates the impact of job assistance on recidivism rates among ex-offenders. The job assistance program, run though the private company America Works, uses a network of employers to place clients. Ex-offenders were randomly assigned to intensive job assistance (treatment group) or the standard program (control group). The intensive program is meant to improve average work readiness for ex-offenders. It reduces the likelihood of subsequent arrest among non-violent ex-offenders, but has little effect on violent ex-offenders. The re-arrest rate for non-violent ex-offenders …

Risk Factors For Workplace Sexual Harassment In Female Truck Drivers, Kimberly Marie Riddle

Theses and Dissertations--Nursing

Sexual harassment is one of the most common forms of workplace violence in the United States. Sexual harassment is defined as unwanted verbal and physical behaviors of a sexual nature (e.g., physical advances, requests for sexual favors, inappropriate sexist or sexual comments or jokes, pornography, or other unwanted conduct) that creates an uncomfortable working environment or interferes with the employee’s job responsibilities. In general, it is estimated that nearly one in every two women have experienced sexual harassment at the workplace over their lifetime. In male-dominated occupations, such as truck driving, law enforcement, firefighting, and construction, females may have a …

Dexamethasone Serum Concentrations After Intravenous Administration In Horses During Race Training, S. Mcclure, C. K. Fenger, Bradley Brown, George Maylin, W. Duer, L. Dirikolu, Kimberly Brewer, Jacob Machin, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Dexamethasone (DXM) sodium phosphate is a widely used corticosteroid for inflammatory conditions in horses, regulated in racing jurisdictions in the USA by a 0.005 ng/ml serum/plasma threshold. This study seeks to describe serum concentrations of DXM at 48 and 72 h after intravenous administration of 20 mg DXM sodium phosphate over 1 to 5 days, and to identify a possible source of DXM overages. 74 horses (39 Thoroughbreds, 13 Standardbreds, 22 Quarter Horses) in active race training received 20 mg DXM sodium phosphate. Serum was collected before injection, at 48 and 72 h post last injection, and analysed by LC/MS-MS …

2020 Kentucky River Watershed Watch: Annual Report, Malissa Mcalister, Steven Evans

Kentucky River Watershed Watch

No abstract provided.