Digital Commons Network^™

Limitations In American Adults’ Awareness Of And Beliefs About Alcohol As A Risk Factor For Cancer, Marc T. Kiviniemi, Heather Orom, Jennifer L. Hay, Erika A. Waters

Health, Behavior & Society Faculty Publications

Alcohol is a carcinogen. Recommendations to reduce alcohol use to lower cancer risk are increasingly common. However, neither the beliefs of US adults about alcohol consumption and cancer risk, nor factors influencing those beliefs, are well understood. We used data from the 2019 Health Information National Trends Survey (analysis N = 4,470) to examine beliefs about whether drinking too much alcohol increases cancer risk. We compared those beliefs to beliefs for three other health problems, and examined whether believing alcohol is a cancer risk factor was related to demographics, risk perceptions, other beliefs about the nature of cancer, and alcohol …

A Cis-Acting Mutation In The Pxabcg1 Promoter Is Associated With Cry1ac Resistance In Plutella Xylostella (L.), Jianying Qin, Fan Ye, Linzheng Xu, Xuguo Zhou, Neil Crickmore, Xiaomao Zhou, Youjun Zhang, Zhaojiang Guo

Entomology Faculty Publications

The molecular mechanisms of insect resistance to Cry toxins generated from the bacterium Bacillus thuringiensis (Bt) urgently need to be elucidated to enable the improvement and sustainability of Bt-based products. Although downregulation of the expression of midgut receptor genes is a pivotal mechanism of insect resistance to Bt Cry toxins, the underlying transcriptional regulation of these genes remains elusive. Herein, we unraveled the regulatory mechanism of the downregulation of the ABC transporter gene PxABCG1 (also called Pxwhite), a functional midgut receptor of the Bt Cry1Ac toxin in Plutella xylostella. The PxABCG1 promoters of Cry1Ac-susceptible and Cry1Ac-resistant strains were …

Role Of Herbicides In Invasive Plant Management Systems, Robert A. Masters, Byron B. Sleugh

IGC Proceedings (1977-2023)

No abstract provided.

The Preparation Temperature Influences The Physicochemical Nature And Activity Of Nanoceria, Robert A. Yokel, Wendel Wohlleben, Johannes Georg Keller, Matthew L. Hancock, Jason M. Unrine, D. Allan Butterfield, Eric A. Grulke

Pharmaceutical Sciences Faculty Publications

Cerium oxide nanoparticles, so-called nanoceria, are engineered nanomaterials prepared by many methods that result in products with varying physicochemical properties and applications. Those used industrially are often calcined, an example is NM-212. Other nanoceria have beneficial pharmaceutical properties and are often prepared by solvothermal synthesis. Solvothermally synthesized nanoceria dissolve in acidic environments, accelerated by carboxylic acids. NM-212 dissolution has been reported to be minimal. To gain insight into the role of high-temperature exposure on nanoceria dissolution, product susceptibility to carboxylic acid-accelerated dissolution, and its effect on biological and catalytic properties of nanoceria, the dissolution of NM-212, a solvothermally synthesized nanoceria …

Understanding And Efficiently Managing Right-To-Take Challenges In Kentucky, Pam Clay-Young, Steve Waddle, Bryan Gibson

Kentucky Transportation Center Research Report

Executive Summary

Kentucky constitutional and statutory law requires that a fair process be in place to let a property owner challenge a condemnor’s right to acquire private property, including a right to appeal the initial decision. Property acquired by a condemnor must be for public use, and property owners have the right to an immediate and expedited hearing on right-to-take. Two of these requirements will remain in place regardless of statutory revisions: the requirement that property must be needed (i.e., not arbitrary) for public use, and the right to one appeal. Only an amendment(s) to the Kentucky Constitution would alter …

A Nation-Wide Survey Of High School Baseball Coaches’ Perceptions Indicates Their Arm Care Programs Play A Role In Injury Prevention, Kyle A. Matsel, E. E. Weiss, R. J. Butler, Matthew C. Hoch, Philip M. Westgate, Terry R. Malone, Timothy L. Uhl

Physical Therapy Faculty Publications

Background
Arm care programs consisting of upper extremity strengthening and stretching have been recommended for injury prevention for pitchers of all ages. There has been no investigation into high school baseball coaches’ usage and perceptions of arm care programs to mitigate physical impairments associated with injuries in baseball players.

Hypothesis/Purpose
The purpose of this study was to investigate the current usage of arm care programs by high school baseball coaches. The primary objective was to determine if coaches use group-based or individualized arm care programs. The secondary objective sought to determine if the use of arm care programs is influenced …

Investigation Of Workflow Processes And Best Practices In Kentucky’S Cdl Program, Valerie Keathley-Helil, Andrew Martin, Brian Howell, Jennifer Walton

Kentucky Transportation Center Research Report

Kentucky’s Division of Driver Licensing maintains the driver history records for all licensed drivers in Kentucky. It serves as the state driver licensing agency and is the locus for meeting the federal CDL requirements under 49 CFR 384. Kentucky relies on FMCSA’s Commercial Driver’s License Program Implementation (CDLPI) grant funding to subsidize salaries for Federally Funded Temporary Labor (FFTL). FFTLs verify and process documents as well as field phone calls from customers. DDL administrators say they cannot meet the CDL reporting timeframes without FFTL labor, but the agency is not able to fund temporary or full-time staff members on its …

Analysis Of Truck Weight Limit Regulations, P. Gayle Marks, Jon Wilcoxson, Bryan Gibson, Chris Van Dyke, Jennifer Walton, Doug Kreis

Kentucky Transportation Center Research Report

In the United States vehicle weight limits are set by laws and regulations enacted at the state and federal levels. On interstates the maximum allowable gross vehicle weight is 80,000 lbs. States use different rules for permitting overdimensional and overweight (OD/OW) vehicles, and most have carve outs that exempt specific commodities from standard weight limits. This results in a complex legal and regulatory landscape that enforcement personnel can find difficult to negotiate. This report discusses strategies that can be adopted in the state of Kentucky to improve enforcement and mitigate infrastructure damage caused by OD/OW loads. After presenting a thorough …

Integration Of Utility Engineering, Coordination, And Highway Design, Tim Taylor, Roy Sturgill, Kenny Franklin, Jeff Jasper, Shani A. Montes Victorio

Kentucky Transportation Center Research Report

State transportation agencies (STAs) strive to deliver highway projects on time and on budget. Utility relocations are frequently cited as a primary reason for delays. This report describes a new approach for enhancing the alignment of utility coordination and design. Researchers crafted this new approach based on the findings of a literature review, an evaluation of how utility coordination milestones and project development milestones are currently aligned at the Kentucky Transportation Cabinet (KYTC), and the experiences of other STAs. KYTC should use this guidance to facilitate implementation of the new approach and an associated Utility Coordination Training and Certification Program. …

Co-Targeting Plk1 And Dnmt3a In Advanced Prostate Cancer, Zhuangzhuang Zhang, Lijun Cheng, Qiongsi Zhang, Yifan Kong, Daheng He, Kunyu Li, Matthew Rea, Jianlin Wang, Ruixin Wang, Jinghui Liu, Zhiguo Li, Chongli Yuan, Enze Liu, Yvonne N. Fondufe-Mittendorf, Lang Li, Tao Han, Chi Wang, Xiaoqi Liu

Toxicology and Cancer Biology Faculty Publications

Because there is no effective treatment for late-stage prostate cancer (PCa) at this moment, identifying novel targets for therapy of advanced PCa is urgently needed. A new network-based systems biology approach, XDeath, is developed to detect crosstalk of signaling pathways associated with PCa progression. This unique integrated network merges gene causal regulation networks and protein-protein interactions to identify novel co-targets for PCa treatment. The results show that polo-like kinase 1 (Plk1) and DNA methyltransferase 3A (DNMT3a)-related signaling pathways are robustly enhanced during PCa progression and together they regulate autophagy as a common death mode. Mechanistically, it is shown that Plk1 …

Yeast Particle Encapsulation Of Scaffolded Terpene Compounds For Controlled Terpene Release, Ernesto R. Soto, Florentina Rus, Hanchen Li, Carli Garceau, Jeffrey Chicca, Mostafa Elfawal, David Gazzola, Martin K. Nielsen, Joseph F. Urban Jr., Raffi V. Aroian, Gary R. Ostroff

Maxwell H. Gluck Equine Research Center Faculty Publications

Terpenes are naturally occurring compounds produced by plants that are of great commercial interest in the food, agricultural, cosmetic, and pharmaceutical industries due to their broad spectra of antibacterial, antifungal, anthelmintic, membrane permeation enhancement, and antioxidant biological activities. Applications of terpenes are often limited by their volatility and the need for surfactants or alcohols to produce stable, soluble (non-precipitated) products. Yeast particles (YPs) are hollow, porous microspheres that have been used for the encapsulation of terpenes (YP terpenes) by passive diffusion of terpenes through the porous YP cell walls. We here report the development of a second generation YP encapsulated …

"Reasonable Expectations," Takings Law Analysis And Administrative Decisions In Light Of Rith V. United States, C. Phillip Wheeler Jr.

Journal of Natural Resources & Environmental Law

No abstract provided.

Lack Of Subject Matter Jurisdiction Or Political Deferral: An Analysis Of Bragg V. West Virginia Coal Association, Crystal Moore

Journal of Natural Resources & Environmental Law

No abstract provided.

Reconsidering Extension: Defining Urban Extension In Kentucky, Kristina D. Hains, Jeff Young, Addie Reinhard, Bryan J. Hains

Community & Leadership Development Faculty Publications

As the vast majority of the population in the United States shifts to dwelling within large population centers, it is necessary to examine the responsibility and role that Cooperative Extension has to serve urban communities. Throughout its history, the land-grant system, through Cooperative Extension, has demonstrated the ability to impact the lives of individual citizens and communities positively. Within this theoretical discussion, we illuminate Cooperative Extension’s responsibility to serve urban communities in the 21st Century and highlight essential milestones in the development of urban Extension throughout the past 100 years. Also, we explore the foundations and relevance of recently developed …

Development Of A Multilevel Intervention To Increase Colorectal Cancer Screening In Appalachia, Aaron J. Kruse-Diehr, Jill M. Oliveri, Robin C. Vanderpool, Mira L. Katz, Paul L. Reiter, Darrell M. Gray Ii, Michael L. Pennell, Gregory S. Young, Bin Huang, Darla Fickle, Mark Cromo, Melinda Rogers, David Gross, Ashley Gibson, Jeanne Jellison, Michael D. Sarap, Tonia A. Bivens, Tracy D. Mcguire, Ann Scheck Mcalearney, Timothy R. Huerta, Saurabh Rahurkar, Electra D. Paskett, Mark B. Dignan

Health, Behavior & Society Faculty Publications

Background

Colorectal cancer (CRC) screening rates are lower in Appalachian regions of the United States than in non-Appalachian regions. Given the availability of various screening modalities, there is critical need for culturally relevant interventions addressing multiple socioecological levels to reduce the regional CRC burden. In this report, we describe the development and baseline findings from year 1 of “Accelerating Colorectal Cancer Screening through Implementation Science (ACCSIS) in Appalachia,” a 5-year, National Cancer Institute Cancer Moonshot^SM-funded multilevel intervention (MLI) project to increase screening in Appalachian Kentucky and Ohio primary care clinics.

Methods

Project development was theory-driven and included the …

Identification Of Binding Regions Of Bilirubin In The Ligand-Binding Pocket Of The Peroxisome Proliferator-Activated Receptor-A (Pparalpha), Darren M. Gordon, Stephen H. Hong, Zachary A. Kipp, Terry D. Hinds Jr.

Pharmacology and Nutritional Sciences Faculty Publications

Recent work has shown that bilirubin has a hormonal function by binding to the peroxisome proliferator-activated receptor-α (PPARα), a nuclear receptor that drives the transcription of genes to control adiposity. Our previous in silico work predicted three potential amino acids that bilirubin may interact with by hydrogen bonding in the PPARα ligand-binding domain (LBD), which could be responsible for the ligand-induced function. To further reveal the amino acids that bilirubin interacts with in the PPARα LBD, we harnessed bilirubin’s known fluorescent properties when bound to proteins such as albumin. Our work here revealed that bilirubin interacts with threonine 283 (T283) …

A Screen Of Fda-Approved Drugs Identifies Inhibitors Of Protein Tyrosine Phosphatase 4a3 (Ptp4a3 Or Prl-3), Dylan R. Rivas, Mark Vincent C. Dela Cerna, Caroline N. Smith, Shilpa Sampathi, Blaine G. Patty, Donghan Lee, Jessica S. Blackburn

Molecular and Cellular Biochemistry Faculty Publications

Protein tyrosine phosphatase 4A3 (PTP4A3 or PRL-3) is highly expressed in a variety of cancers, where it promotes tumor cell migration and metastasis leading to poor prognosis. Despite its clinical significance, small molecule inhibitors of PRL-3 are lacking. Here, we screened 1443 FDA-approved drugs for their ability to inhibit the activity of the PRL phosphatase family. We identified five specific inhibitors for PRL-3 as well as one selective inhibitor of PRL-2. Additionally, we found nine drugs that broadly and significantly suppressed PRL activity. Two of these broad-spectrum PRL inhibitors, Salirasib and Candesartan, blocked PRL-3-induced migration in human embryonic kidney cells …

Forest Development Over A Twenty-Year Chronosequence Of Reforested Urban Sites, Kenton L. Sena, Zachary J. Hackworth, John M. Lhotka

Lewis Honors College Faculty Publications

Urbanization causes a variety of negative ecological impacts, impairing forests, streams, and other ecosystems. While urban forests are the subject of increasing research attention, planted urban forests are less well-understood than remnant forests; however, these systems may be distinct in terms of ecosystem structure and function. The current study investigates a chronosequence of reforested urban sites in Lexington, KY, USA, with a focus on overstory and understory woody plant community characteristics. Monitoring plots were established in each of the 20 sites; tree height, dbh, and species were surveyed for the overstory, and species and height were surveyed for the understory. …

Acute Inflammatory Profiles Differ With Sex And Age After Spinal Cord Injury, Andrew N. Stewart, John L. Lowe, Ethan P. Glaser, Caitlin A. Mott, Ryan K. Shahidehpour, Katelyn E. Mcfarlane, William M. Bailey, Bei Zhang, John C. Gensel

Physiology Faculty Publications

Background

Sex and age are emerging as influential variables that affect spinal cord injury (SCI) recovery. Despite a changing demographic towards older age at the time of SCI, the effects of sex or age on inflammation remain to be elucidated. This study determined the sex- and age-dependency of the innate immune response acutely after SCI.

Methods

Male and female mice of ages 4- and 14-month-old received T9 contusion SCI and the proportion of microglia, monocyte-derived macrophages (MDM), and neutrophils surrounding the lesion were determined at 3- and 7-day post-injury (DPI) using flow cytometry. Cell counts of microglia and MDMs were …

A Survey On Long-Range Wide-Area Network Technology Optimizations, Felipe S. Dantas Silva, Emidio P. Neto, Helder Oliveira, Denis Rosário, Eduardo Cerqueira, Cristiano Both, Sherali Zeadally, Augusto V. Neto

Information Science Faculty Publications

Long-Range Wide-Area Network (LoRaWAN) enables flexible long-range service communications with low power consumption which is suitable for many IoT applications. The densification of LoRaWAN, which is needed to meet a wide range of IoT networking requirements, poses further challenges. For instance, the deployment of gateways and IoT devices are widely deployed in urban areas, which leads to interference caused by concurrent transmissions on the same channel. In this context, it is crucial to understand aspects such as the coexistence of IoT devices and applications, resource allocation, Media Access Control (MAC) layer, network planning, and mobility support, that directly affect LoRaWAN’s …

Technological Trash: Should We Regulate The Disposal Of Cathode Ray Tubes In Waste Facilities?, Kenneth Clay Ratley

Journal of Natural Resources & Environmental Law

No abstract provided.

The Wto And The Environment: The Shrimp Case That Created A New World Order, Laura Yavitz

Journal of Natural Resources & Environmental Law

No abstract provided.

Cleanup Of Contaminated Oyster Beds Under The Clean Water Act And Louisiana's Environmental And Water Quality Laws, Erinn W. Neyrey, M. Michelle Marney

Journal of Natural Resources & Environmental Law

No abstract provided.

Pneumo Abex Corp. V. High Point, Thomasville & Denton Railroad, And Cercla "Arranger Liability", Jason C. Kuhlman

Journal of Natural Resources & Environmental Law

No abstract provided.

Dancing Around The Issue Of Fifra Preemption: Does It Really Still Matter That The Supreme Court Has Not Made A Decision?, Sandra L. Feeley

Journal of Natural Resources & Environmental Law

No abstract provided.

A Work Place Surveillance: Who's Watching?, Mark E. Heath

Journal of Natural Resources & Environmental Law

No abstract provided.

Tmdls For Nonpoint Sources In Kentucky: The Potential Impact Of Pronsolino V. Marcus, Henry L. Stephens Jr., Monica Dias

Journal of Natural Resources & Environmental Law

No abstract provided.

Mootness And Attorneys' Fees Under The Clean Water Act In The Old Timer, Inc. V. Blackhawk-Central City Sanitation District, Wanda A. Dotson

Journal of Natural Resources & Environmental Law

No abstract provided.

Ascriptive Characteristics And Perceptions Of Impropriety In The Rule Of Law: Race, Gender, And Public Assessments Of Whether Judges Can Be Impartial, Yoshikuni Ono, Michael A. Zilis

Political Science Faculty Publications

Perceptions of procedural fairness influence the legitimacy of the law and because procedures are mutable, reforming them can buttress support for the rule of law. Yet legal authorities have recently faced a distinct challenge: accusations of impropriety based on their ascriptive characteristics (e.g., gender, ethnicity). We study the effect of these traits in the context of the U.S. legal system, focusing on the conditions under which citizens perceive female and minority judges as exhibiting impropriety and how this compares with perceptions of their white and male counterparts. We find that Americans use a judge's race and gender to make inferences …

Measuring The Impact Of The Affordable Care Act Medicaid Expansion On Access To Primary Care Using An Interrupted Time Series Approach, Elizabeth A. Brown, Brandi M. White, Walter J. Jones, Mulugeta Gebregziabher, Kit N. Simpson

Health and Clinical Sciences Faculty Publications

BACKGROUND: The Patient Protection and Affordable Care Act of 2010, commonly referred to as the Affordable Care Act (ACA), was created to increase access to primary care, improve quality of care, and decrease healthcare costs. A key provision in the law that mandated expansion of state Medicaid programme changed when states were given the option to voluntarily expand Medicaid. Our study sought to measure the impact of ACA Medicaid expansion on preventable hospitalization (PH) rates, a measure of access to primary care.

METHODS: We performed an interrupted time series analysis of quarterly hospitalization rates across eight states from 2012 to …