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Validation Of An Automated Behavior Monitoring Collar, And Evaluation Of Heat Stress On Lactating Dairy Cow Behavior With Access To A Free Choice Soaker, Lori Nicole Grinter

Theses and Dissertations--Animal and Food Sciences

Precision dairy technologies (PDT) are becoming more accessible and are therefore becoming more common on commercial dairy farms and in dairy research. Prior to any use of PDT, one should understand the precision, accuracy and bias of the device by a validations studies before interpreting the behavior measurements. Thus, the objective of the first section of my thesis is to validate ruminating, feeding and resting measurements of a behavior monitoring collar used in the second section. Precision dairy technology is used in heat stress studies to compare behavior of cows exposed to different heat stress treatments or abatement strategies. Heat …

Responses Of Bovine Pituitary Transcriptome Profiles To Consumption Of Toxic Tall Fescue And Forms Of Selenium In Vitamin-Mineral Mixes, Qing Li

Theses and Dissertations--Animal and Food Sciences

The first goal of the current research was to determine whether gene expression profiles differed between whole pituitaries of growing beef steers grazing pastures containing high (HE) or low (LE) amounts of toxic endophyte-infected tall fescue. The global (microarray analysis) and selected targeted (RT-PCR) mRNA expression patterns of pituitaries collected from beef steers (BW = 266 ± 15.5 kg) that had been randomly assigned to undergo summer-long grazing (89 to 105 d) of either HE (0.52 ppm ergot alkaloids) or LE (< 0.03 ppm ergot alkaloids) pastures were compared. Gene expression data were subjected to one-way ANOVA. The pituitaries of HE steers had 542 differentially expressed genes, and the pattern of altered gene expression was dependent on treatment. Targeted RT-PCR analysis corroborated these findings, including decreased expression of DRD2, PRL, POU1F1, GAL, and VIP and that of POMC and PCSK1, respectively. Canonical pathway analysis (Integrated Pathway Analysis, IPA) identified HE-dependent alteration in signaling of additional pituitary-derived hormones, including growth hormone and GnRH. In conclusion, consumption of endophyte-infected tall fescue alters the pituitary transcriptome profiles of steers in a manner consistent with their negatively affected physiological parameters. The second goal of this project was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral (VM) mix would differentially alter pituitary transcriptome profiles in growing beef steers (BW = 183 ± 34 kg) commonly grazing an endophyte-infected tall fescue (HE) pasture. Steers were randomly selected from herds of fall-calving cows grazing HE pasture and consuming VM mixes that contained 35 ppm Se as either ISe, OSe, or MIX forms. Steers were weaned, depleted of Se for 98 d, and subjected to summer-long common grazing of a 10.1 ha HE pasture containing 0.51 ppm ergot alkaloids. Steers were assigned (n = 8) to the same Se-form treatments on which they were raised. Selenium treatments were administered by daily top-dressing 85 g of VM mix onto 0.23 kg soyhulls, using in-pasture Calan gates. Pituitaries were collected at slaughter and changes in global (microarray) and selected (RT-PCR) mRNA expression patterns determined. The effects of Se treatment on relative gene expression were subjected to one-way ANOVA. The form of Se affected the expression of 542 annotated genes. Integrated Pathway Analysis found a canonical pathway network between prolactin and POMC/ACTH/ α-MSH synthesis-related proteins, and that mitochondrial dysfunction was a top-affected canonical pathway. Targeted RT-PCR analysis found that the relative abundance of mRNA encoding prolactin and POMC/ACTH/ α-MSH synthesis-related proteins was affected by the form of Se, as were mitochondrial dysfunction-related proteins OSe steers appeared to have a greater prolactin synthesis capacity vs. ISe steers through decreased dopamine receptor D2 signaling, whereas MIX steers had a greater prolactin synthesis capacity and release potential by increasing TRH concentrations than ISe steers. OSe steers also had a greater ACTH and α-MSH synthesis potential than ISe steers. We conclude that form of Se in VM mixes affected genes responsible for prolactin and POMC/ACTH/α-MSH synthesis, and mitochondrial function in pituitaries of growing beef steers commonly grazing an HE pasture. The third goal was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral (VM) mix would differentially alter selenoprotein profiles in pituitaries and livers of growing beef steers commonly grazing an endophyte-infected tall fescue (HE) pasture (i.e., the same steers used in Goal 2). The effects of Se treatment on relative gene expression were subjected to one-way ANOVA. The mRNA content of 6 selenoproteins in the pituitary was affected by Se treatments, along with two selenoprotein P receptors, whereas the expression of two selenoproteins was altered in the liver. We conclude that the change in selenoprotein gene expression in pituitaries indicates that OSe steers have a greater potential capacity to manage against oxidative damage, maintain cellular redox balance, and have a better quality control of protein-folding in their pituitaries than ISe steers. The change in selenoprotein gene expression by the liver indicates that MIX steers have a greater redox signaling capacity and capacity to manage oxidative damage than ISe steers.

Behavioral And Physiological Adaptations Associated With Feed Intake During Transitioning Cattle To High-Grain Diets, Amanda M. Mclean

Theses and Dissertations--Animal and Food Sciences

Transitioning cattle from a high-forage to a high-concentrate diet increases the risk for ruminal acidosis and is often related to decreased feed intake, which compromises animal health and performance. Since control of feed intake and rumen motility are closely related, we hypothesized that a reduction in rumen motility may be associated with a reduction in feed intake during this transition. Computer programs were created to analyze feed disappearance and rumen pressure data for feeding behavior as well as identification and characterization of rumen contractions, respectively. This method enabled timely analysis of large datasets and removed subjectivity associated with manual analysis. …

An Appraisal Of The Effect Of Nursing Location On Weaning Weight Of Piglets And Its Dependence On The Feeding Of Essential Oils To Sows, Shannon L. Dierking

Theses and Dissertations--Animal and Food Sciences

The objective of these studies was to evaluate the effect of piglet nursing location on weaning weight, and its dependence on essential oil supplementation on sow and piglet performances.

Piglets that nursed anterior teat pairs had heavier weaning weights and higher gain for the lactation period. Additionally, piglet birthweight did not impact their overall teat selection and nursing location. These results provide some insight into the biological aspects of sow milk production, and implied that milk yield may vary between teat pairs along the udder line.

Supplementation of essential oils (EO) during late gestation and lactation had no effect on …

The Ecology And Behavior Of Spring Migrating Indiana Bats (Myotis Sodalis), Piper Lee Roby

Theses and Dissertations--Animal and Food Sciences

Migration of animals has been studied for decades and has included everything from large terrestrial and pelagic mammals traveling thousands of kilometers to many types of birds flying through several countries to insects going through multiple life cycles in a single migration. The migration of bats has been studied in broad terms to gather coarse information such as distance traveled, connecting summer and winter habitat, and a general understanding of timing. However, only recently have researchers begun to understand the specifics of bat migration including physiology and fine resolution behavior.

Using nine years of spring migration data collected on VHF …

Vital Rates And Habitat Selection Of Bull Elk (Cervus Canadensis Nelsoni) In Southeast Kentucky, John Tyler Hast

Theses and Dissertations--Animal and Food Sciences

Globally, male ungulate species are heavily managed for their sporting and trophy qualities. North American elk (Cervus canadensis) are typically managed using a male-biased harvest regime, placing increased chances of mortality on males in these hunted populations. To manage for trophy quality animals that typically represent older age classes, wildlife managers have implemented many age-biased harvest regulations, including spike-only tags and antler point restrictions. Many of these age-biased harvest regulations have fallen short of their desired goal of producing older bull elk. Consequently, the consensus has evolved to center on an overall reduction in harvest pressure.

The state …

Association Between Pm2.5 And The Coexistence Of Chronic Obstructive Pulmonary Disease And Coronary Heart Disease, Shan Gao

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Aim: To identify PM2.5 as a risk factor for chronic obstructive pulmonary disease (COPD) and coronary heart disease (CHD) respectively, and to explore the magnitude of the association of PM2.5 with the coexistence of CHD and COPD, by using the combined dataset of 1999-2014 EPA Air Data and 2017 BRFSS SMART data (age 18 and older).

Methods: Cross-sectional study was designed to identify the association between PM2.5 exposure and COPD and CHD. The PM2.5 exposure values were adapted from the county-level EPAAir Data, the yearly mean values of PM2.5 were used to determine the association. Characteristics of sex, age, race, …

A Cross Sectional Study Of Drinking Water Quality And Health Effects In The El Bua Tsáchila Community, Santo Domingo De Los Tsáchilas, Ecuador, Dannette Cook

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Background: Access to clean water and sanitation is known to decrease childhood mortality, improve health outcomes, and decrease risk of water-borne diseases. However, access to clean water is often restricted in impoverished or indigenous communities. This cross-sectional study evaluated the primary source of drinking water for forty homes, the Est. Cheche river, a local spring, and a local school in the TsáChila indigenous community of El Bua, Santo Domingo, Ecuador. This analysis focused on the presence of coliforms and E. coli in the water source (well or city/piped), the physical parameters of the water and the type of sanitation …

A Brain-Computer Interface For Closed-Loop Sensory Stimulation During Motor Training In Patients With Tetraplegia, Sarah Helen Thomas

Theses and Dissertations--Biomedical Engineering

Normal movement execution requires proper coupling of motor and sensory activation. An increasing body of literature supports the idea that incorporation of sensory stimulation into motor rehabilitation practices increases its effectiveness. Paired associative stimulation (PAS) studies, in which afferent and efferent pathways are activated in tandem, have brought attention to the importance of well-timed stimulation rather than non-associative (i.e., open-loop) activation. In patients with tetraplegia resulting from spinal cord injury (SCI), varying degrees of upper limb function may remain and could be harnessed for rehabilitation. Incorporating associative sensory stimulation coupled with self-paced motor training would be a means for supplementing …

A Possible Link Between R-Wave Amplitude Alternans And T-Wave Alternans In Ecgs, Sahar Alaei

Theses and Dissertations--Biomedical Engineering

Sudden Cardiac Death (SCD) is the largest cause of natural deaths in the USA, accounting for over 300,000 deaths annually. The major reason for SCD is Ventricular Arrhythmia (VA). Therefore, there is need for exploration of approaches to predict increased risk for VA. Alternans of the T wave in the ECG (TWA) is widely investigated as a potential predictor of VA, however, clinical trials show that TWA has high negative predictive value but poor positive predictive value. A possible reason that TWA has a large number of false positives is that a pattern of alternans known as concordant alternans, may …

Calibrated Short Tr Recovery Mri For Rapid Measurement Of Brain-Blood Partition Coefficient And Correction Of Quantitative Cerebral Blood Flow, Scott William Thalman

Theses and Dissertations--Biomedical Engineering

The high prevalence and mortality of cerebrovascular disease has led to the development of several methods to measure cerebral blood flow (CBF) in vivo. One of these, arterial spin labeling (ASL), is a quantitative magnetic resonance imaging (MRI) technique with the advantage that it is completely non-invasive. The quantification of CBF using ASL requires correction for a tissue specific parameter called the brain-blood partition coefficient (BBPC). Despite regional and inter-subject variability in BBPC, the current recommended implementation of ASL uses a constant assumed value of 0.9 mL/g for all regions of the brain, all subjects, and even all species.

The …

Noninvasive Multimodal Diffuse Optical Imaging Of Vulnerable Tissue Hemodynamics, Mingjun Zhao

Theses and Dissertations--Biomedical Engineering

Measurement of tissue hemodynamics provides vital information for the assessment of tissue viability. This thesis reports three noninvasive near-infrared diffuse optical systems for spectroscopic measurements and tomographic imaging of tissue hemodynamics in vulnerable tissues with the goal of disease diagnosis and treatment monitoring. A hybrid near-infrared spectroscopy/diffuse correlation spectroscopy (NIRS/DCS) instrument with a contact fiber-optic probe was developed and utilized for simultaneous and continuous monitoring of blood flow (BF), blood oxygenation, and oxidative metabolism in exercising gastrocnemius. Results measured by the hybrid NIRS/DCS instrument in 37 subjects (mean age: 67 ± 6) indicated that vitamin D supplement plus aerobic training …

Improving Safety Performance Of Highway Maintenance Crews Through Pre-Task Safety Toolbox Talks, Zamaan Al-Shabbani

Theses and Dissertations--Civil Engineering

The dangerous work environment in the construction industry and the inherent high risks associated with construction work make it the focus of safety training and regulations. Highway construction and maintenance has unique hazards but seemingly less directly applicable safety standards, regulations, and programs. Department of Transportation (DOT) employees working in highway maintenance are exposed to a variety of unique hazards specifically associated with their work and not relating to the adjacent traffic. Yet, highway construction and maintenance work has not received sufficient attention in terms of safety research and programs. The lack of safety training and education in highway construction …

Use Of Functionalized Bimetallic Membranes For Treatment Of Contaminated Groundwater At A Hazardous Waste Site In Kentucky, Lucy C. Pacholik

Theses and Dissertations--Civil Engineering

Providing access to clean drinking water will continue to be a challenge for civil engineers for generations to come. Since many communities around the world rely on groundwater as a source of drinking water, remediation technologies must be implemented at sites where groundwater contamination exists due to years of mismanagement of hazardous waste. Using nanosized zero-valent metals such as iron and zinc embedded within and on the surface of functionalized (PAA) membrane filters has shown to be an effective dechlorination technique for contaminated groundwater. Introducing a noble metal such as Pd or Ni increases reaction rates by acting a catalyst …

Decomposing A Watershed’S Nitrate Signal Using Spatial Sampling And Continuous Sensor Data, Evan Clare

Theses and Dissertations--Civil Engineering

Watershed features, physiographic setting, geology, climate, and hydrologic processes combine to produce a time-variant nutrient concentration signal at the watershed outlet. Anthropogenic influences, such as increased agricultural pressures and urbanization, have increased overall nutrient loadings delivered to the fluvial network. The impact of such increased nutrient loadings on Kentucky’s drinking water remains a potential threat to the region.

By coupling spatial sampling of nitrate concentrations in surface water with contemporary nutrient and water quality sensor technology, a decomposition of the Upper South Elkhorn watershed’s nitrate signal and an estimation of source timing and loading in the watershed was completed. The …

Biological Selenium Control: Selenium Reduction By Shigella Fergusonii Strain Tb42616 And Pantoea Vagans Strain Ewb32213-2 In Bioreactor Systems, Yuxia Ji

Theses and Dissertations--Civil Engineering

Se(VI) and Se(IV), as the two major species of selenium in water, are toxic to aquatic lives and may cause adverse health effects to humans at high levels. Biological reduction of Se(VI) is a two-stage process first from Se(VI) to Se(IV) and then from Se(IV) to Se(0) with potential accumulation of the more toxic Se(IV) due to the slower rate of the second stage.

Selenium reduction was first evaluated with batch cultures of Shigella fergusonii strain TB42616 (TB) and Pantoea vagans strain EWB32213-2 (EWB) isolated in our laboratory from sludge and coal slurry sediment samples, respectively. In order to facilitate …

Synthesis, Design, And Evaluation Of The Fluorescent Detection Of Polychlorinated Biphenyls(Pcbs) In Aqueous System, Irfan Ahmad

Theses and Dissertations--Chemical and Materials Engineering

The exposure to halogenated persistent organic pollutants (POPs), such as polychlorinated biphenyls (PCBs), has been linked to numerous inflammatory diseases, including diabetes, cancer and lowered immune response. PCBs have low solubility in water, and they interact with other contaminants, making their detection quite challenging. While, there have been several attempts at improving the ease of detection and sensing of PCBs, gas chromatography-mass spectrometry (GC-MS) remains the gold standard. However, despite its ubiquitous use, GC-MS is a challenging technique that requires high skill and careful sample preparation, which are time-consuming and costly. As such, there is still a need to develop …

Bioinformatic And Experimental Analyses Of Axolotl Regeneration, Nour W. Al Haj Baddar

Theses and Dissertations--Biology

Salamanders have an extraordinary ability to regenerate appendages after loss or amputation, irrespective of age. My dissertation research explored the possibility that regenerative ability is associated with the evolution of novel, salamander-specific genes. I utilized transcriptional and genomic databases for the axolotl to discover previously unidentified genes, to the exclusion of other vertebrate taxa. Among the genes identified were multiple mmps (Matrix metalloproteases) and a jnk1/mapk8 (c-jun-N-terminal kinase) paralog. MMPs function in extracellular matrix remodeling (ECM) and tissue histolysis, processes that are essential for successful regeneration. Jjnk1/mapk8 plays a pivotal role in regulating transcription in response to cellular stress stimuli, …

Variability In Groundwater Flow And Chemistry In The Houzhai Karst Basin, Guizhou Province, China, Joshua M. Barna

Theses and Dissertations--Earth and Environmental Sciences

Understanding how karst aquifers store and transmit water and contaminants is an ongoing problem in hydrogeology. Flowpath and recharge heterogeneity contribute to the complexity of these systems. This thesis explores karst-conduit connectivity and water chemistry variability in the Houzhai catchment in Guizhou province, China. Artificial tracer tests were conducted during both the monsoon and dry seasons to understand temporal variability in connectivity and water velocity between karst features. Multiple flowpaths through the catchment are activated during the monsoon season and partially abandoned during the dry season. Additionally, gradient reversals during monsoonal high-flow events and as a result of pumping can …

The Forestry Reclamation Approach: Measuring Sediment Mass Accumulation Rates In Reclaimed Mine Lands And Naturally Regenerated Logged Forests Of Eastern Kentucky, William E. Bond

Theses and Dissertations--Earth and Environmental Sciences

The spread of surface coal mining has resulted in loss of forests in the Appalachian region. The Forestry Reclamation Approach (FRA) was developed to provide guidance for restoring forests on reclaimed mined land. This study hypothesizes that the FRA will result in larger magnitude of sediment accumulation rates in reclaimed mine sites compared to those reclaimed using grassland reclamation. Three sediment cores and six trenches were sampled within four reclaimed mined and three previously logged sites in eastern Kentucky. Samples were processed for radionuclides, grain-size, stable isotopes (δ¹³C), and POC. LIDAR data were used to identify valley …

Nineteenth-Century Pets And The Politics Of Touch, Valerie L. Stevens

Theses and Dissertations--English

Nineteenth-Century Pets and the Politics of Touch examines texts of the era in which both humans and animals find empowerment at the point of physical encounter. I challenge contemporary perceptions of human-pet relationships as sweetly affectionate by focusing on touch. I uncover an earlier interest in the close reciprocal relationships between human and nonhuman animals, arguing that these nineteenth-century thinkers presented what I call a “politics of touch,” in which intimate and often jarring physical encounters allow for mutuality and autonomy. I first turn to Charlotte Brontë’s Shirley (1849) and protective violence, a condoned ferocity that frequently unites and guards …

But What Has Helga Crane To Do With The West Indies? Plantation Afterlives In The Black Atlantic, Rachel Mckenzie Carr

Theses and Dissertations--English

“But What Has Helga Crane to Do with the West Indies? Plantation Afterlives in the Black Atlantic” situates the emergence of the southern gothic in modernist American and Caribbean works as a response to the shifting cultural narrative of the plantation in the twentieth century. In this project, I argue that the plantation seeps out of its place and time to haunt landscapes it may never have touched and times in which slavery is long over. While the plantation system is broadly recognized as a literary, political, and cultural force in nineteenth-century literary studies, I conceive it is also a …

History Speaks From The Soil: A Case Study Of Commons Enclosure In The Clearance Era On North And South Uist, Anna Rachel Herrington

Theses and Dissertations--History

This thesis argues that commons enclosure in the Clearance Era on the Uist island group in the Outer Hebrides of Scotland was a direct result of the Clearances on those islands in the 18^th and 19^th centuries and how the enclosure of commons on these islands was catastrophic to those communities who had functioned, worked, and thrived in those regions for millennia. Commons and commons systems are those resources such as land, water, and produce either from agriculture or natural harvesting which contribute to human habitation and existence in a particular geographic area. Commons and commons systems on …

Agricultural Livelihoods In Harlan County: A Case Study Approach Of Two Farms, Heather Kinney

Theses and Dissertations--Community & Leadership Development

This thesis explores agricultural livelihoods in Harlan County with two case studies in order to challenge dominant narratives about Eastern Kentucky. Harlan County, and Appalachia more broadly, is often written about in terms of its relationship to extractive industries. Absentee landownership in Appalachia has been well documented, especially in the case of coal counties. However, the relationship between extraction and agricultural livelihoods in Appalachia warrants more attention. The story of agricultural livelihoods in the region is often pushed to the periphery much like the practices of “hillside” farming. While geography makes much of the land unsuitable for farming in Harlan …

Investigating The Feasibility Of Using Mesoporous Silica Particles To Deliver Glucose During Anaerobic Fermentation, Megan E. Walz

Theses and Dissertations--Biosystems and Agricultural Engineering

The study presented herein investigated a potential low-energy method to separate and concentrate glucose from a lignocellulosic hydrolysate. The motivation for this method was twofold: 1) to provide the fermentation microorganism an optimal glucose concentration and 2) to supply a fermentation media free of inhibitory compounds. Two sizes of porous silica particles (with 7 nm and 2.3 nm pores) were synthesized and their ability to adsorb glucose from solution confirmed. Next, the ability of two different microorganisms, Saccharomyces cerevisiae and Streptococcus bovis, to utilize sugars adsorbed to the porous silica particles was investigated.

Both the 7 nm and 2.3 …

Evaluation Of Air Movement In Equestrian Facilities And On Equine Athletes, Staci Elaine Mcgill

Theses and Dissertations--Biosystems and Agricultural Engineering

Ventilation and air movement are important aspects of animal agriculture and is frequently neglected in equine facilities. This paper discusses three different studies that examine different components of ventilation and air movement. One is a fan orientation study which examines how fans impact the stall environment, the second is a cooling study questioning whether forced air speed across a horse increases the rate of cool out after intense exercise, and the last is a survey examining ventilation, air quality, and health concerns in indoor arenas.

The stall fan study took place over two summers with the goal of determining how …

Design And Testing Of A Wind Energy Harnessing System For Forced Convective Drying Of Grain In Low Wind Speed, Warm And Humid Climates, Francis Akumabi Agbali

Theses and Dissertations--Biosystems and Agricultural Engineering

Forced convective drying using a wind turbine mechanically connected to a ventilation fan was hypothesized for low cost and rapid grain drying in developing countries. The idea was tested using an expandable wind turbine blade system with variable pitch, at low wind speeds in a wind tunnel. The design was based on empirical and theoretical models embedded in a graphical user interface (GUI) created to estimate airflow-power requirements for drying ear corn. Output airflow (0.0016 - 0.0052 m³kg^-1s^-1) increased within the study wind speed range (2.0 - 5.5 m/s). System efficiency peak (8.6%) was …

Rapid No• Measures In Rat Nucleus Accumbens And Frontal Cortex Following Nasal Administration Of Nitroglycerin, Victoria A. Scott

Theses and Dissertations--Medical Sciences

Nitric Oxide (NO) is a powerful endogenous free radical that has numerous biological functions including vasodilation and serves as a post synaptic second messenger in the central nervous system (CNS). Numerous studies implicate NO• involvement in CNS disorders such as schizophrenia and drug abuse. These studies address the direct in vivo determination of an FDA-approved NO• donor (nitroglycerin) on extracellular levels of NO• in the frontal cortex and core of the nucleus accumbens in a lightly anesthetized rat. State-of-the-art in vivo amperometric recording techniques coupled with a novel 4-channel low noise pre-amplifier system and new generation microelectrode arrays (MEAs) will …

The Stimulation Of Dendritic Cells By Cationic Lipids, John Peyton Bush

Theses and Dissertations--Medical Sciences

The discovery that cationic lipids can independently stimulate the immune system has generated interest in their potential as vaccine adjuvants. Here, we show that the cationic lipid R-DOTAP can independently stimulate type 1 interferon production in dendritic cells in both primary culture and immortalized cell culture. Levels of type 1 interferon production are cell line-dependent and limited in vitro by lipid-induced cell death. We show that cationic lipids can independently activate TLR-7 and TLR-9, suggesting a mechanism for type 1 interferon induction. This TLR-stimulatory activity is not restricted to R-DOTAP and can be extended to other similar cationic lipids in …

Integrin Α5Β1 As A Novel Target With The Small Peptide, Atn-161, In The Treatment Of Ischemic Stroke, Danielle Nichele Edwards

Theses and Dissertations--Neuroscience

Stroke is the 5^th leading cause of death and the leading cause of disability in the United States, but there are only two available therapies, tissue plasminogen activator and endovascular thrombectomy. As both therapies focus on removal of the clot, the subsequent pathologic processes, i.e. inflammation, cerebrovascular breakdown, ATP depletion, etc. are left untreated, contributing to worsened patient outcome. Many clinical trials have unsuccessfully attempted to address these mechanisms. The blood-brain barrier (BBB), a system of non-fenestrated endothelial cells, extracellular matrix, and astrocytic endfeet, is significantly impacted after ischemic stroke in its role of preventing the free movement of …