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Condensed Tannin Concentration And Herbage Accumulation Of A White Clover Bred For Increased Floral Condensed Tannin, V. T. Burggraaf, S. L. Woodward, D. R. Woodfield, E. R. Thom, G. C. Waghorn, P. D. Kemp May 2023

Condensed Tannin Concentration And Herbage Accumulation Of A White Clover Bred For Increased Floral Condensed Tannin, V. T. Burggraaf, S. L. Woodward, D. R. Woodfield, E. R. Thom, G. C. Waghorn, P. D. Kemp

IGC Proceedings (1977-2023)

White clover is a high quality feed for ruminants, however, its high protein content results in excessive urea excretion in urine and can cause bloat, reducing its potential value for animal production. The condensed tannins (CT) in some forages can reduce these problems, but plants may have poor agronomic performance. White clover produces CT in its flower heads, but herbage CT concentrations are normally too low to benefit animals. This paper reports CT concentrations and herbage accumulation over 2 years of an experimental line of white clover (HT) selected for increased flowering and floral CT concentrations.

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Effects Of Simulated High-Sugar Forages On Grazing Behaviour Of Sheep, Gerald P. Cosgrove, A. J. Parsons May 2023

Effects Of Simulated High-Sugar Forages On Grazing Behaviour Of Sheep, Gerald P. Cosgrove, A. J. Parsons

IGC Proceedings (1977-2023)

Sustaining an optimum composition in mixed-species pasture and in the diet of grazing animals is constrained by many factors. Altering the concentration of water soluble carbohydrate (WSC) of one species to improve its nutritional value for animals may not deliver the benefits in the assumed manner if it also affects preference and the balance of species in the pasture. Furthermore, associated changes in other constituents (e.g. fibre, protein) make it difficult to attribute animal responses solely to the manipulated trait (e.g. Lee et al. 2000). The objective of this study was to simulate changes in the concentration of water soluble …

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High Floral Tannin White Clover Reduces Rumen Ammonia Concentrations In Dairy Cows, V. T. Burggraaf, G. C. Waghorn, S. L. Woodward, D. R. Woodfield, E. R. Thom, P. D. Kemp May 2023

High Floral Tannin White Clover Reduces Rumen Ammonia Concentrations In Dairy Cows, V. T. Burggraaf, G. C. Waghorn, S. L. Woodward, D. R. Woodfield, E. R. Thom, P. D. Kemp

IGC Proceedings (1977-2023)

White clover produces high quality forage for ruminant production, but it results in high rumen ammonia concentrations, indicating extensive protein degradation. The excess ammonia is absorbed through the rumen and excreted as urea in urine, at a cost to the animal and the environment. Condensed tannins (CT) contained in some forages reduce proteolysis in the rumen, which can lead to increased amino acid absorption and therefore improved animal performance. White clover produces CT in its flower heads, but concentrations are normally too low to benefit animals. This paper reports on comparisons of rumen ammonia concentrations in dairy cows grazing HT …

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Variation In Protein Quality Of Forage Legumes During Spring Growth, M. Gierus, J. Kleen, F. Taube May 2023

Variation In Protein Quality Of Forage Legumes During Spring Growth, M. Gierus, J. Kleen, F. Taube

IGC Proceedings (1977-2023)

The utilisation of forage legumes in combination with reduced N inputs as fertilisers is an alternative for the production of high quality forage. Although white clover (WC) is widely used in grassland and has a high content of crude protein (CP), a combination of this high CP content with a high proportion of rapidly-available proteins and fast degradation rate in the rumen may reduce the efficiency of N use by ruminants in comparison with other forage legumes. The objective of the present study was to investigate the variation in content of CP fractions A, B and C in different forage …

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Effects Of Particle Size In Forage Samples For Protein Breakdown Studies, G. Pichard, C. Tapia May 2023

Effects Of Particle Size In Forage Samples For Protein Breakdown Studies, G. Pichard, C. Tapia

IGC Proceedings (1977-2023)

Coupling ruminal processes of hydrolysis and synthesis continues to be a research issue where more progress is needed. This requires the development of good protein assessment methods, particularly when representing the breakdown processes that occur in fresh pastures eaten by herbivores. Laboratory analyses need to deal with small and homogeneous samples, but the mechanical reduction of particle size may not reflect the actual digestion kinetics occurring when the original fresh forage is consumed. Such physical traits may alter the release of non-structural compounds and the penetration of microbial enzymes (Boudon et al., 2002). The objective of this work was …

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Ruminal Dry Matter, Neutral Detergent Fibre And Acid Detergent Fiber Degradation Kinetics Of Dominant Pasture Forages In Kurdestan Province Of Iran, A. A. Sadeghi, P. Shawrang, A. Nikkhah May 2023

Ruminal Dry Matter, Neutral Detergent Fibre And Acid Detergent Fiber Degradation Kinetics Of Dominant Pasture Forages In Kurdestan Province Of Iran, A. A. Sadeghi, P. Shawrang, A. Nikkhah

IGC Proceedings (1977-2023)

Neutral detergent fiber (NDF) is a major chemical component of forages and its degradability (dNDF) is an essential parameter in predicting their energetic value. Moreover, dNDF has been used in models to estimate the physical fill of fibrous feeds in the rumen and, therefore, the intake capacity of animals. As the available information on the nutritive value of pastoral forages is limited a study was undertaken to measure chemical composition and cell wall degradation kinetics of eight pasture forages in the rumen.

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Herbage Mass And In Situ Dry Matter Ruminal Degradation Kinetics Of Brachiaria Spp, E. Valencia-Chin, A. A. Rodríguez-Carías, R. Ramos-Santana, S. Pagán-Riestra May 2023

Herbage Mass And In Situ Dry Matter Ruminal Degradation Kinetics Of Brachiaria Spp, E. Valencia-Chin, A. A. Rodríguez-Carías, R. Ramos-Santana, S. Pagán-Riestra

IGC Proceedings (1977-2023)

In Puerto Rico, Brachiaria decumbens cv. Basilisk has been promoted as a potential forage for acid soils and humid areas, but with limited success. Recently, B. brizantha cv. Marandú and a hybrid (B. brizantha x B. Ruziziensis) cv. Mulato were introduced for evaluation on acid soils and as a potential replacement for cv. Basilisk, but little information is available on yield performance under grazing or nutritive value. The objective of this study was to assess herbage mass and nutritive value of grazed pastures consisting of Basilisk, Marandú, and Mulato and determine the rate of in situ dry matter …

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Effects Of Forage Species And Stage Of Maturity On In Situ Disappearance Of Organic Matter And Fibre Fractions, H. S. Hussein May 2023

Effects Of Forage Species And Stage Of Maturity On In Situ Disappearance Of Organic Matter And Fibre Fractions, H. S. Hussein

IGC Proceedings (1977-2023)

Nutrient utilisation by ruminants is altered by the forage species and its maturity. Maturity is the major factor affecting forage morphology and quality. Forage quality is reduced with maturity due to a decrease in the leaf:stem ratio and an increase in fibre components (Ugherughe, 1986). Improving forage utilisation by ruminants depends on accurate measurements of their nutritive value by using in vitro and in situ methods. The objective of this study was to assess the nutritive value (i.e., extent of in situ disappearance of organic matter [OM], neutral detergent fibre [NDF], and acid detergent fibre [ADF]) of four grass species …

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The Effect Of Nitrogen Fertiliser And Season On The In Situ Degradability Of Irish Perennial Ryegrass In Cattle, V. Olsson, J. J. Murphy, F. P. O'Mara, K. O'Connell, J. Humphreys, F. J. Mulligan May 2023

The Effect Of Nitrogen Fertiliser And Season On The In Situ Degradability Of Irish Perennial Ryegrass In Cattle, V. Olsson, J. J. Murphy, F. P. O'Mara, K. O'Connell, J. Humphreys, F. J. Mulligan

IGC Proceedings (1977-2023)

In light of increasing environmental and economic pressure on agriculture to utilise resources more efficiently, protein feeding and its effects are fundamentally important. As grazed grass is the predominant feed in Irish dairy and beef cattle production systems, it is necessary to establish protein values for different grass varieties and cultivars fed. It is also important to investigate the extent of ruminal nitrogen (N) degradability for these grasses since this characteristic greatly influences environmentally damaging urinary N excretion.

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The Effect Of Cow-Diet On The Fermentation Of Forages, J. L. Burke, G. C. Waghorn, S. L. Woodward, I. M. Brookes May 2023

The Effect Of Cow-Diet On The Fermentation Of Forages, J. L. Burke, G. C. Waghorn, S. L. Woodward, I. M. Brookes

IGC Proceedings (1977-2023)

In vitro fermentation of fresh forages minced to resemble chewed material have enabled net proteolysis and volatile fatty acid (VFA) production to be measured using rumen inocula from a cow fed lucerne hay (Burke et al., 2000). However both cow and diet affect the rumen cellulolytic bacterial populations (Weimer et al., 1999) and are able to influence in vitro digestion kinetics (Mertens et al., 1998). The objective of this study was to measure cow-diet effects on in vitro digestion and fermentation of perennial ryegrass (P; Lolium perenne), sulla (S; Hedysarum coronarium), maize (M; Zea maize …

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Fibre Degradation Rate Of Perennial Ryegrass Varieties Measured Using Three Techniques: In Situ Nylon Bag, In Vivo Rumen Evacuation And In Vitro Gas Production, H. Z. Taweel, B. M. Tas, B. A. Williams, A. Elgersma, J. Dijkstra, S. Tamminga May 2023

Fibre Degradation Rate Of Perennial Ryegrass Varieties Measured Using Three Techniques: In Situ Nylon Bag, In Vivo Rumen Evacuation And In Vitro Gas Production, H. Z. Taweel, B. M. Tas, B. A. Williams, A. Elgersma, J. Dijkstra, S. Tamminga

IGC Proceedings (1977-2023)

In Western Europe, perennial ryegrass is the most widely used grass species for grazing cattle, because of its high productivity, palatability and nutritive value. However, the low dry matter intake (DMI) of perennial ryegrass pasture has been identified as a major factor limiting milk production of high producing dairy cows. Altering the chemical, physical and mechanical characteristics that contribute to its low DMI through grass breeding and the choice of variety may be a way forward in trying to maximise its DMI. This study aimed to examine whether perennial ryegrass varieties differ in their NDF degradation rates (kdNDF).

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Perennial Ryegrass Variety Differences In Nutritive Value Characteristics, T. J. Gilliland, R. E. Agnew, A. M. Fearon, F. E. A. Wilson May 2023

Perennial Ryegrass Variety Differences In Nutritive Value Characteristics, T. J. Gilliland, R. E. Agnew, A. M. Fearon, F. E. A. Wilson

IGC Proceedings (1977-2023)

Animal grazing performance at grass is predominately determined by herbage intake rates, with high yielding dairy cows requiring up to 20 kg/d DM within a limited grazing time (Gibb, 1998). Grass nutritional factors such as seasonal patterns in digestibility and water-soluble carbohydrate levels have been linked to animal productivity (Davies et al., 1991), while sward surface height, herbage mass, bulk density and green leaf mass have been shown to promote high grazing intake (Barrett et al., 2001). Furthermore, fatty acid profiles have been shown to improve the unsaturated fatty acid composition of milk, with potential human health benefits …

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Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger May 2023

Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger

IGC Proceedings (1977-2023)

Tropical grasses are the primary staple diet of most of the domesticated ruminants in the tropical and subtropical regions of the world. However, dry matter (DM) digestibility (DMD) and intake levels of these C4 grasses are considerably low, partly because of high lignin contents. Ferulic acid also impedes fibre digestion in such forages due to formation of cross linkages with digestible xylans. Certain esterase enzymes have been shown to cleave ferulic acid cross linkages in wheat bran but no studies have examined whether such enzymes can be used to increase the digestion of tropical grasses. Therefore, the objective of …

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Mating Ewes On Condensed Tannin-Containing Forages Increases Ewe Reproductive Rate And Reduces Lamb Mortality, T. N. Barry, C. A. Ramirez-Restrepo, E. L. Mcwilliam, N. Lopez-Villalobos, P. D. Kemp May 2023

Mating Ewes On Condensed Tannin-Containing Forages Increases Ewe Reproductive Rate And Reduces Lamb Mortality, T. N. Barry, C. A. Ramirez-Restrepo, E. L. Mcwilliam, N. Lopez-Villalobos, P. D. Kemp

IGC Proceedings (1977-2023)

Action of condensed tannin (CT) reduces forage protein degradation in the rumen and increases the absorption of amino acids from the small intestine (Barry & McNabb 1999). This paper reports the effects of grazing ewes on two CT-containing forages during mating upon ewe reproductive rate and lamb mortality.

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Ruminal Proteolysis In Forages With Distinct Endopeptidases Activities, G. Pichard, C. Tapia, R. Larraín May 2023

Ruminal Proteolysis In Forages With Distinct Endopeptidases Activities, G. Pichard, C. Tapia, R. Larraín

IGC Proceedings (1977-2023)

Improving livestock efficiency in utilization of nitrogen resources continues to be a major environmental and economic objective. Zhu et al. (1999) have shown that plant endopeptidases are activated as a response to cutting stress. Previous work in our laboratory explored over 300 entries of forage genotypes and found a broad diversity in enzymatic activity by means of hydrolysis in gelatine and direct autolysis assays in forage tissues. The objective of this work was to assess if the species previously identified as having high or low endopeptidase activity, would behave consistently when exposed to ruminal microbial proteolysis.

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The Death Of Plants In Animals, M. K. Theodorou, A. Kingston-Smith, P. Morris May 2023

The Death Of Plants In Animals, M. K. Theodorou, A. Kingston-Smith, P. Morris

IGC Proceedings (1977-2023)

It is necessary first to understand some of the basic concepts associated with the digestion of the plant biomass within the rumen when considering mechanisms for altering/enhancing N-conversion efficiency in the forage-fed ruminant. Although it is generally assumed that breakdown of plant proteins in the rumen is mediated by microbial enzymes, there is increasing evidence to suggest that both plant and microbial proteases are active during degradation of ingested fresh forage (Beha et al., 2002; Kingston-Smith & Theodorou, 2000; Kingston-Smith et al., 2003, 2004). After fresh plant biomass enters the rumen and prior to extensive plant cell wall …

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Anthelmintic Effects Of Sericea Lespedeza Hay Fed To Goats Infected With Haemonchus Contortus, S. A. Shaik, T. H. Terrill, J. E. Miller, B. Kouakou, G. Kannan, R. M. Kaplan, J. M. Burke, J. A. Mosjidis May 2023

Anthelmintic Effects Of Sericea Lespedeza Hay Fed To Goats Infected With Haemonchus Contortus, S. A. Shaik, T. H. Terrill, J. E. Miller, B. Kouakou, G. Kannan, R. M. Kaplan, J. M. Burke, J. A. Mosjidis

IGC Proceedings (1977-2023)

Infection with gastrointestinal nematodes (GIN), particularly Haemonchus contortus, is the major hindrance to economic goat production in the southern USA. Grazing forages high in condensed tannins (CT) or adding purified CT to the diet has been shown to reduce numbers of parasite eggs in sheep and goat faeces (Min & Hart, 2003). An alternative to grazing is feeding hay from CT-containing forages to livestock to reduce the effects of GIN (Shaik et al., 2004). The purpose of the current study was to test potential anthelmintic effects of feeding sericea lespedeza [(Lespedeza cuneata (Dum.-Cours.) G. Don] hay to …

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Effect Of Pre-Planting Seed Treatment Options On Dormancy Breaking And Germination Of Ziziphus Mucronata, Abubeker Hassen, N. F. G. Rethman, Willem A. Van Niekerk May 2023

Effect Of Pre-Planting Seed Treatment Options On Dormancy Breaking And Germination Of Ziziphus Mucronata, Abubeker Hassen, N. F. G. Rethman, Willem A. Van Niekerk

IGC Proceedings (1977-2023)

Ziziphus mucronata (Buffalo thorn) is a multipurpose tree, widely adapted to a range of ecological conditions and tolerant of extreme climatic conditions, including frost and drought (Venter & Venter, 1996). It is a valuable fodder tree for livestock and game animals, especially in the drier parts of Africa (Rothauge et al. 2003). Similar to many other leguminous species, establishment is constrained by low and erratic germination of the seed, which has been attributed mainly to the physical barrier of the stony endocarp and dormancy associated with seed coat impermeability . This experiment aimed to compare the suitability of various …

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Seed Production And Resource Allocation In Three Cultivars Of Achnatherum Hymenoides, Nevada, Usa, Barry L. Perryman, C. A. Busso, Hudson A. Glimp May 2023

Seed Production And Resource Allocation In Three Cultivars Of Achnatherum Hymenoides, Nevada, Usa, Barry L. Perryman, C. A. Busso, Hudson A. Glimp

IGC Proceedings (1977-2023)

Plant production is partially determined by resource allocation among various organs (Monsi & Murata, 1970), however, studies on dry matter partitioning among different plant organs are scarce in general (Marceli, 1996), and lacking in Achnatherum hymenoides. This study compared dry matter production and partitioning among three commercial cultivars (Paloma, Nezpar and Rimrock) of A. hymenoides and identified growth and developmental characteristics that could indicate potential seed production. In addition, the relationship between an organ weight as a percent of total aerial plant biomass was assessed.

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Effect Of Three Legumes Containing Different Condensed Tannin Concentrations On The In Vitro Formation Of The Pastoral Flavour Compound; Skatole, N. M. Schreurs, M. H. Tavendale, G. A. Lane, T. N. Barry, W. C. Mcnabb May 2023

Effect Of Three Legumes Containing Different Condensed Tannin Concentrations On The In Vitro Formation Of The Pastoral Flavour Compound; Skatole, N. M. Schreurs, M. H. Tavendale, G. A. Lane, T. N. Barry, W. C. Mcnabb

IGC Proceedings (1977-2023)

Feeding legumes, such as white clover (Trifolium repens), results in higher intakes and increased animal production compared to grasses (Ulyatt, 1981). Skatole is produced in the rumen from plant protein fermentation and is associated with undesirable pastoral flavours in meat (Young et al. 2002). Feeding white clover causes a greater skatole concentration in the rumen compared to perennial ryegrass (Lolium perenne) or Lotus corniculatus, as the protein in white clover is highly soluble and rapidly degraded (Schreurs et al., 2004). The condensed tannins (CT) in Lotus species slow protein degradation in the rumen (Aerts …

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Shearing Strength And Chemical Composition In The Selection For Quality In Brachiaria Brizantha, Cacilda B. Valle, F. E. Torres, B. Lempp May 2023

Shearing Strength And Chemical Composition In The Selection For Quality In Brachiaria Brizantha, Cacilda B. Valle, F. E. Torres, B. Lempp

IGC Proceedings (1977-2023)

Selection of quality pasture forages for productivity, nutritive value and animal performance require long-term, expensive trials. Simpler and accurate techniques to detect quality differences among genotypes have been proposed (Mackinnon et al., 1988; Hughes et al., 2000). This paper discusses the use of shearing strength in Brachiaria brizantha ecotypes to correlate physical traits with chemical composition: the objective being the identification of cultivars of improved quality forage suitable to the savannas of Brazil.

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Growth Characteristics Of Superior Lines Of Zoysia Grass (Zoysia Japonica) And Development Of Its Dna Markers, Y. W. Rim, K. Y. Kim, M. J. Kim, B. R. Sung, Y. C. Lim, E. S. Chung May 2023

Growth Characteristics Of Superior Lines Of Zoysia Grass (Zoysia Japonica) And Development Of Its Dna Markers, Y. W. Rim, K. Y. Kim, M. J. Kim, B. R. Sung, Y. C. Lim, E. S. Chung

IGC Proceedings (1977-2023)

Demand for turf grass has steadily increased for recreation and sport fields after the 2002 Korea-Japan World Cup in Korea. Zoysia grass has the advantage of easy management, including low water and fertiliser requirement, but has limitations such as low recovery, low shoot density and short green period (Kim et al., 1999). Objectives of this research were to select superior lines in the collected clones, compare the superior lines of zoysia grass with other standard cultivars for growth characteristics and to develop the DNA markers of superior lines.

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Quality Legume-Based Forage Systems For Contrasting Environments: Cost Action 852, A. Helgadottir, John Connolly, M. Fothergill, R. P. Collins, Claudio Porqueddu, Andreas Lüscher, M. T. Sebastia, Michael Kreuzer, M. Wachendorf May 2023

Quality Legume-Based Forage Systems For Contrasting Environments: Cost Action 852, A. Helgadottir, John Connolly, M. Fothergill, R. P. Collins, Claudio Porqueddu, Andreas Lüscher, M. T. Sebastia, Michael Kreuzer, M. Wachendorf

IGC Proceedings (1977-2023)

Agricultural systems that reduce environmental degradation, sustain agricultural productivity and economic viability, maintain stable rural communities, enhance the quality of life and respond to increasing demand for livestock products are promoted in developed countries. Though major challenges exist, forage legumes, adapted to a wide range of soil types, climatic conditions and management systems, will become increasingly important components of sustainable agricultural production systems in Europe. Temporal and spatial variation in legume performance often occurs. Compared to pure grass systems, legume-based systems may lead to increased N losses. To what extent ruminants can use the protein from forage legumes, and whether …

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New Cultivars For High Quality, Persistent Legume-Grass Pastures In The Southern Usa, J. H. Bouton May 2023

New Cultivars For High Quality, Persistent Legume-Grass Pastures In The Southern Usa, J. H. Bouton

IGC Proceedings (1977-2023)

In the southern USA one cannot depend on perennial legume-grass pasture systems to have persistently high nutritive quality. 'Jesup' tall fescue, a cultivar with better persistence in the region than 'Kentucky 31', was re-infected with a non-ergot alkaloid producing strain of the Neotyphodium coenophialum fungal endophtye (MaxQTM) and found to give persistence equal to Jesup with its endemic strain (E+), but without animal toxins (Bouton et al., 2002). 'Durana' white clover (Bouton et al., 2004) was developed from regional ecotypes of Trifolium repens f. hollandicum germplasm and found to improve animal performance in both E+ and …

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Comparing Yield And Quality Of Milk From Dairy Cows Fed Stockpiled Annual Ryegrass (Lolium Multiflorum L.) And Cereal Rye (Secale Cereale L.), L. E. Meinhardt, R. L. Kallenbach May 2023

Comparing Yield And Quality Of Milk From Dairy Cows Fed Stockpiled Annual Ryegrass (Lolium Multiflorum L.) And Cereal Rye (Secale Cereale L.), L. E. Meinhardt, R. L. Kallenbach

IGC Proceedings (1977-2023)

Stockpiling annual ryegrass and cereal rye provides a low cost substitute to hay and creates an excellent source of feed during winter (Kallenbach et al., 2003). In addition to lowering feed costs, grazing increases the conjugated linoleic acid (CLA) content of milk compared to feeding hay. Previous research suggested that forage species might differ in their ability to alter milk CLA content during the growing season (Wu et al., 1997). However, research is needed to determine if different forage species used for winter and early spring grazing impacts the CLA content of milk. The objective of this experiment …

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A Grazing Method To Solve The Lack Of Pastures In The Dry Season Of Tropical Areas With Long Periods Of Drought, R. O. Martinez, R. Tuero, M. F. Díaz May 2023

A Grazing Method To Solve The Lack Of Pastures In The Dry Season Of Tropical Areas With Long Periods Of Drought, R. O. Martinez, R. Tuero, M. F. Díaz

IGC Proceedings (1977-2023)

Rotational grazing systems used in tropical areas in Latin America do not solve the great difference in pasture availability between the dry and the rainy season. The main studies on rational grazing (Voisin, 1963) were performed in temperate areas where the deficit of feeds in winter may only be solved with external feeds such as forages and silages produced out of the grazing system. The objective of this work was to demonstrate that it is possible to maintain pasture availability throughout the year with the use of a Pennisetum purpureum clone (Cuba CT-115) adapted to grazing (Martínez et al., …

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Accelerating Colorectal Cancer Screening And Follow-Up Through Implementation Science (Accsis) In Appalachia: Protocol For A Group Randomized, Delayed Intervention Trial, Electra D. Paskett, Aaron J. Kruse-Diehr, Jill M. Oliveri, Robin C. Vanderpool, Darrell M. Gray Ii, Michael L. Pennell, Bin Huang, Gregory S. Young, Darla Fickle, Mark Cromo, Mira L. Katz, Paul L. Reiter, Melinda Rogers, David A. Gross, Vickie Fairchild, Wendy Xu, Angela Carman, Jean M. Walunis, Ann Scheck Mcalearney, Timothy R. Huerta, Saurabh Rahurkar, Erika Biederman, Mark B. Dignan May 2023

Accelerating Colorectal Cancer Screening And Follow-Up Through Implementation Science (Accsis) In Appalachia: Protocol For A Group Randomized, Delayed Intervention Trial, Electra D. Paskett, Aaron J. Kruse-Diehr, Jill M. Oliveri, Robin C. Vanderpool, Darrell M. Gray Ii, Michael L. Pennell, Bin Huang, Gregory S. Young, Darla Fickle, Mark Cromo, Mira L. Katz, Paul L. Reiter, Melinda Rogers, David A. Gross, Vickie Fairchild, Wendy Xu, Angela Carman, Jean M. Walunis, Ann Scheck Mcalearney, Timothy R. Huerta, Saurabh Rahurkar, Erika Biederman, Mark B. Dignan

UK CARES Faculty Publications

Appalachian regions of Kentucky and Ohio are hotspots for colorectal cancer (CRC) mortality in the USA. Screening reduces CRC incidence and mortality; however, screening uptake is needed, especially in these underserved geographic areas. Implementation science offers strategies to address this challenge. The aim of the current study was to conduct multi-site, transdisciplinary research to evaluate and improve CRC screening processes using implementation science strategies. The study consists of two phases (Planning and Implementation). In the Planning Phase, a multilevel assessment of 12 health centers (HC) (one HC from each of the 12 Appalachian counties) was conducted by interviewing key informants, …

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Combination Therapy With Trastuzumab And Niraparib: Quantifying Early Proliferative Alterations In Her2+ Breast Cancer Models, Ameer Mansur, Patrick N. Song, Yun Lu, Andrew C. Burns, Luke Sligh, Eddy S. Yang, Anna G. Sorace May 2023

Combination Therapy With Trastuzumab And Niraparib: Quantifying Early Proliferative Alterations In Her2+ Breast Cancer Models, Ameer Mansur, Patrick N. Song, Yun Lu, Andrew C. Burns, Luke Sligh, Eddy S. Yang, Anna G. Sorace

Markey Cancer Center Faculty Publications

HER2–targeted treatments have improved survival rates in HER2+ breast cancer patients, yet poor responsiveness remains a major clinical obstacle. Recently, HER2+ breast cancer cells, both resistant and responsive to HER2–targeted therapies, have demonstrated sensitivity to poly–(ADP– ribose) polymerase (PARP) inhibition, independent of DNA repair deficiencies. This study seeks to describe biological factors that precede cell viability changes in response to the combination of trastuzumab and PARP inhibition. Treatment response was evaluated in HER2+ and HER2– breast cancer cells. Further, we evaluated the utility of 3′–Deoxy–3′–[18F]–fluorothymidine positron emission tomography ([18F]FLT–PET) imaging for early response assessment in a HER2+ patient derived xenograft …

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Spatial Metabolomics Reveals Glycogen As An Actionable Target For Pulmonary Fibrosis, Lindsey R. Conroy, Harrison A. Clarke, Derek B. Allison, Samuel Santos Valenca, Qi Sun, Tara R. Hawkinson, Lyndsay E. A. Young, Juanita E. Ferreira, Autumn V. Hammonds, Jaclyn B. Dunne, Robert J. Mcdonald, Kimberly J. Absher, Brittany Dong, Ronald C. Bruntz, Kia H. Markussen, Jelena A. Juras, Warren J. Alilain, Jinze Liu, Matthew S. Gentry, Peggi M. Angel, Christopher M. Waters, Ramon C. Sun May 2023

Spatial Metabolomics Reveals Glycogen As An Actionable Target For Pulmonary Fibrosis, Lindsey R. Conroy, Harrison A. Clarke, Derek B. Allison, Samuel Santos Valenca, Qi Sun, Tara R. Hawkinson, Lyndsay E. A. Young, Juanita E. Ferreira, Autumn V. Hammonds, Jaclyn B. Dunne, Robert J. Mcdonald, Kimberly J. Absher, Brittany Dong, Ronald C. Bruntz, Kia H. Markussen, Jelena A. Juras, Warren J. Alilain, Jinze Liu, Matthew S. Gentry, Peggi M. Angel, Christopher M. Waters, Ramon C. Sun

Saha Cardiovascular Research Center Faculty Publications

Matrix assisted laser desorption/ionization imaging has greatly improved our understanding of spatial biology, however a robust bioinformatic pipeline for data analysis is lacking. Here, we demonstrate the application of high- dimensionality reduction/spatial clustering and histopathological annotation of matrix assisted laser desorption/ionization imaging datasets to assess tissue metabolic heterogeneity in human lung diseases. Using metabolic features identified from this pipeline, we hypothesize that metabolic channeling between glycogen and N-linked glycans is a critical metabolic process favoring pulmonary fibrosis progression. To test our hypothesis, we induced pulmonary fibrosis in two different mouse models with lysosomal glycogen utilization deficiency. Both mouse models displayed …

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Development And Characterization Of Nanobodies That Specifically Target The Oncogenic Phosphatase Of Regenerating Liver-3 (Prl-3) And Impact Its Interaction With A Known Binding Partner, Cnnm3, Caroline N. Smith, Kyle Kihn, Zachary A. Williamson, K. Martin Chow, Louis B. Hersh, Konstantin V. Korotkov, Daniel Deredge, Jessica S. Blackburn May 2023

Development And Characterization Of Nanobodies That Specifically Target The Oncogenic Phosphatase Of Regenerating Liver-3 (Prl-3) And Impact Its Interaction With A Known Binding Partner, Cnnm3, Caroline N. Smith, Kyle Kihn, Zachary A. Williamson, K. Martin Chow, Louis B. Hersh, Konstantin V. Korotkov, Daniel Deredge, Jessica S. Blackburn

Markey Cancer Center Faculty Publications

Phosphatase of Regenerating Liver-3 (PRL-3) is associated with cancer progression and metastasis. The mechanisms that drive PRL-3’s oncogenic functions are not well under- stood, partly due to a lack of research tools available to study this protein. We have begun to address these issues by developing alpaca-derived single domain antibodies, or nanobo- dies, targeting PRL-3 with a KD of 30–300 nM and no activity towards highly homologous family members PRL-1 and PRL-2. We found that longer and charged N-terminal tags on PRL-3, such as GFP and FLAG, changed PRL-3 localization compared to untagged protein, indicating that the nanobodies may provide …

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