Digital Commons Network^™

Gene Regulatory Networks Of Agl15 A Plant Mads Transcription Factor, Cong Zhu

University of Kentucky Doctoral Dissertations

Plant embryogenesis is an intriguing developmental process that is controlled by many genes. AGAMOUS Like 15 (AGL15) is a MADS-domain transcriptional regulator that accumulates preferentially during this stage. However, at the onset of this work it was unknown which genes are regulated by AGL15 or how AGL15 is regulated. This dissertation is part of the ongoing effort to understand the biological roles of AGL15. To decipher how AGL15 functions during plant development, a chromatin immunoprecipitation (ChIP) approach was adapted to obtain DNA fragments that are directly bound by AGL15 in vivo. Putative AGL15 targets were isolated, and binding and regulation …

Mechanism Of Cancer Selective Apoptosis By Par-4, Sushma Gurumurthy

University of Kentucky Doctoral Dissertations

Despite distinct dissimilarities, diverse cancers express several common pro-tumorigenic traits. We present here evidence that the pro-apoptotic protein Par-4 utilizes one such common tumorigenic trait to become selectively activated and induce apoptosis in cancer cells. Elevated PKA activity noted in cancer cells activated the apoptotic function of ectopic Par-4 or its SAC domain, which induces apoptosis selectively in cancer cells and not in normal or immortalized cells. PKA preferentially phosphorylated Par-4 at the T155 residue within the SAC domain in cancer cells. Moreover, pharmacological-, peptide- or siRNA-mediated inhibition of PKA activity in cancer cells resulted in abrogation of both T155 …

Role Of Matrix Metalloproteinase-2 In Therosclerosis And Abdominal Aortic Aneurysms In Apolipoprotein E Deficient Mice, Jing Huang

University of Kentucky Doctoral Dissertations

Matrix metalloproteinase-2 (MMP-2, gelatinase A, type IV collagenase) is a member of a family of zinc-dependent metalloendopeptidases that functions in the degradation of elastin, collagens, and other components of extracellular matrix (ECM). Both secretion and activation of MMP-2 are elevated in human atherosclerotic lesions and abdominal aortic aneurysms (AAA). In this dissertation project, we sought to test the hypothesis that MMP-2 plays a critical role in both atherosclerosis and AAA. We also sought to determine the detailed mechanism. We first examined the atherosclerosis and AngII-induced AAAs development in MMP-2-/- x apolipoprotein (apoE)-/- mice in vivo. It was surprising that MMP-2 …

Permissiveness Of Selected Cell Lines To Equine Arteritis Virus: Establishment, Characterization, And Significance Of Persistent Infection In Hela Cells, Jianqiang Zhang

University of Kentucky Doctoral Dissertations

A major goal of this research was to evaluate a variety of cell lines for theirpermissiveness to equine arteritis virus (EAV) infection and then identify the mechanismthat restricts EAV infection in certain cell lines. The cell lines BHK-21, RK-13, andC2C12 were found to support productive infection with EAV strain VBS53, whereasHela, Hep-2, and L-M cell lines exhibited limited susceptibility to infection with thisvirus. In the course of the study, it was found that the Hela cell line became moresusceptible to infection with EAV strain VBS53 after extended serial passage. Therespective cell lines were referred to as Hela High (passage 170-221) …

Interactions Between Selenium And Polychlorinated Biphenyls (Pcbs), Divinia Nolasco Stemm

University of Kentucky Doctoral Dissertations

This study investigated the interaction between polychlorinated biphenyls (PCBs) and selenium to explain the mechanism involved that could affect selenium metabolism and its anti-cancer property. PCBs congeners and mixtures were previously found to reduce hepatic Se and Se-dependent glutathione peroxidase activity. I hypothesized that certain PCB congeners affect selenium metabolism in the rat liver resulting in diminished antioxidant capacity of selenoproteins, which could alter the ability of Se to protect against PCBs induced tumor promotion. In the first study, the influence of 3,3,4,4-tetrachlorobiphenyl (PCB 77) on hepatic Se and glutathione peroxidase (GPx1) activity as well as cytochrome P450 1A1 induction …

Kentucky Feeder Cattle Price Analysis: Models For Price Predictions And Grazing Management, Roger Wayne Eldridge

University of Kentucky Master's Theses

Kentucky plays an important role in the complex U.S. beef cattle industry. Thisstudy focused on the feeder cattle production sector of Kentucky's beef cattle industry.Primarily a cow-calf state with a substantial backgrounding sector, Kentucky is a largesupplier of feeder cattle to the cattle finishing sector. Price relationships within themarket for Kentucky feeder cattle were examined using historical price data fromKentucky livestock auction markets. This research revealed many interesting pricerelationships that Kentucky producers may use in order to increase the profitability of thecow-calf and/or backgrounding operations. A segment of this research includes aGrazing Management Decision Tool which was constructed to enable …

Surface Modification Of Plga Biomaterials For Site-Directed Immobilization Of Growth Factors, Jessica Bennett Lynn Sharon

University of Kentucky Master's Theses

Biodegradable polymer materials, specifically poly(lactic-co-glycolide) (PLGA) can be used as bone replacements for bone regeneration. Scaffolds can be prepared to be porous to induce bone growth into a scaffold so that it is replaced with natural tissue as the polymer degrades. However, simply using PLGA will result in formation of scar tissue rather than regeneration of natural bone. Therefore focus has turned to attaching growth factors to the PLGA molecules to elicit a specific cellular response when the implant is placed in the body. Site-directed immobilization utilizes specific groups on both the biomaterial and biomolecule so that growth factors can …

Sexual Conflict And Density Dependence In The Western Mosquitofish, Gambusia Affinis (Poeciliidae), Chad Smith

University of Kentucky Master's Theses

Sexual conflict occurs when individuals of one sex express traits that reduce the fitness of their mates. Males of many species harass females to gain copulations, which benefits males by increasing the number of offspring they sire but imposes energetic and opportunity costs on the females they harass. This thesis examined the fitness costs of sexual harassment to females, the energetic costs of mating to males, and the factors influencing the intensity of male competition for mates in the western mosquitofish Gambusia affinis. I quantified male and female behavior, four female fitness components (number of offspring per female, embryo number, …

Comparative Analysis Of Five Complete Ambystomatid Salamander Mitochondrial Genomes, Amy K. Samuels

University of Kentucky Master's Theses

In this study, mitochondrial transcript information from a recent EST project wasextended to obtain complete mitochondrial genome sequence for 5 tiger salamandercomplex species (Ambystoma mexicanum, A. t. tigrinum, A. andersoni, A. californiense,and A. dumerilii). For the first time, aspects of mitochondrial transcription in arepresentative amphibian are described, and then complete mitochondrial sequencedata are used to examine salamander phylogeny at both deep and shallow levels ofevolutionary divergence. The available mitochondrial ESTs for A. mexicanum (N=2481)and A. t. tigrinum (N=1205) provided 92% and 87% coverage of the mitochondrialgenome, respectively. Complete mitochondrial sequences for all species were rapidlyobtained by using long distance PCR …

An Examination Of Dietary Amendments To Affect Phosphorus Utilization In Growing Pigs, Jorge Hernan Agudelo-Trujillo

University of Kentucky Doctoral Dissertations

For economical and ecological reasons, efficiency and profitability ofswine production relies heavily on the way pigs utilize key nutrients such as P,which is considered a potential pollutant of water ecosystems. Although cerealgrains and oilseed meals contain enough P to fulfill the biological needs of pigs,most of this P is tightly bound as phytate. As pigs do not have enough phytase(PHY) to cleave P from phytate, it is excreted in the feces. To prevent adeficiency, diets have traditionally been supplemented with highly availableinorganic sources of P. Today, an environmentally-friendly alternative is tosupplement diets with PHY.Growth promoting antibiotics are also used to …

Identification And Characterization Of Contact Sites Between Human Follicle Stimulating Hormone And The Follicle Stimulating Hormone Receptor, Johann Sohn

University of Kentucky Doctoral Dissertations

Follicle stimulating hormone (FSH) comprises an ?? subunit and a ?? subunit,whereas the FSH receptor consists of two halves with distinct functions, the N-terminalextracellular exodomain and C-terminal membrane associated endodomain. FSH initiallybinds to exodomain, and the resulting FSH/exodomain complex modulates the endodomainand generates signal. However, it has been difficult to determine which subunit of FSHcontacts the exodomain or endodomain, and in what orientation FSH interacts with them.To address these crucial issues, the receptor was Ala-scanned and the hormone subunitswere probed with photoaffinity labeling with receptor peptides corresponding to the Nterminalregion of the exodomain and exoloop 3 of the endodomain. The …

A Comparison Between Masticatory Muscle And Temporomandibular Joint Pain Patients With Regard To The Prevalence And Impact Of Post-Traumatic Stress Disorder Symptoms, Elizangela Bertoli

University of Kentucky Master's Theses

The purpose of this study was to evaluate masticatory muscle (MM) and temporomandibular joint (TMJ) pain patients regarding the prevalence of Posttraumatic Stress Disorder (PTSD) symptoms, and evaluate the level of psychological dysfunction and its relationship to PTSD symptoms in these patients. This study included 445 adult patients (male = 42, female = 403). Psychological questionnaires included the Symptom Check List-90-Revised (SCL-90-R), the Multidimensional Pain Inventory, the Pittsburgh Sleep Quality Index and the PTSD Check List Civilian. The total sample of patients was divided into two major groups: The MM group (n=242) and TMJ group (n=203). Each group was divided …

Some Effects Of Cadmium On Select Crayfish In The Family Cambaridae, Andrew Joseph Wigginton

University of Kentucky Doctoral Dissertations

A series of acute (96h) toxicity tests were conducted on six species of crayfish inthe family Cambaridae. Toxicity values fell into a sensitive group comprising Orconectesplacidus and Procambarus acutus (LC 50= 0.368mg Cd/L - 0.487mg Cd/L; LC10= 0.048mg Cd/L - 0.092 mg Cd/L) and tolerant group comprising O. juvenilis, O. virilis, P.alleni, and P. clarkii (LC 50= 2.44 mg Cd/L - 3.30 mg Cd/L; LC10= 0.386 mg Cd/L -0.947 mg Cd/L). For juvenile crayfish, the LC50 and LC10 values were as follows: O.juvenilis, 0.060 and 0.014 mg Cd/L; O. placidus, 0.037 and 0.002 mg Cd/L; P. clarkii,0.624 and 0.283 mg …

Insights Into Enzymatic Manipulations Of Nucleic Acids, Rashada Corine Alexander

University of Kentucky Doctoral Dissertations

This dissertation details three studies dealing with the manipulation of nucleicacids. In the first investigation, each of the four natural nucleobases were analyzed for theability to serve as a universal template at the ligation junction of a T4 DNA ligasereaction. This resulted in the first instance of sequence-independent ligation catalyzed byany DNA ligase. Although all of the nucleobases display universal templatingcapabilities, thymidine and guanosine provided the most effective results. In addition,lowered MgCl2 and ATP concentrations, as well as the inclusion of DMSO, also aided inthe sequence-independent ligation reported here. In the course of these studies, currentmethods of removing urea from …

Characterization Of Pollutant Response In Teleosts With Varying Degrees Of Pollutant Sensitivity, Benjamin Frederick Brammell

University of Kentucky Doctoral Dissertations

Cytochrome P4501A (CYP1A), a xenobiotic metabolizing enzyme found in allvertebrates, is highly induced following exposure to a number of organic contaminants.Several populations of teleosts residing in highly contaminated areas have been found toexhibit resistance to the toxic effects of contaminants, a condition characterized byreduced expression of CYP1A.Within this work I demonstrated that expression of CYP1A mRNA, protein, andactivity in caged rainbow trout (Oncorhynchus mykiss) was an effective biomarker ofpolychlorinated biphenyl (PCB) contamination. Furthermore, through the use of bothlaboratory and field studies, I demonstrated that several species inhabiting a PCBcontaminated site exhibited either acquired (Ameiurus natalis) or natural (Lepomiscyanellus) resistance to …

Methods Development In Biological Mass Spectrometry: Applications In Small Molecule Research And Proteomics, Taufika Islam Williams

University of Kentucky Doctoral Dissertations

Technological developments have enabled mass spectrometry (MS) to evolve asone of the most versatile, sensitive and widely used analytical methods. Key areas ofresearch in biological MS include the development of analyte-selective MSmethodologies, along with the design of MS compatible separation technology. Analytesof interest range from small, biologically active molecules in disease progressionresearch, to macromolecules such as proteins, in proteomics investigations. Advances inthese areas are vital to maintaining the level of sophistication that has become thebenchmark for MS analyses.Mass spectrometry has found a permanent station in disease progression studies,particularly in biomarker discovery. This is especially true for Alzheimer's disease (AD),a condition …

Redox Proteomics Identification Of Oxidatively Modified Proteins And Their Pharmacological Modulation: Insight Into Oxidative Stress In Brain Aging, Age-Related Cognitive Impairment, Hung Fai Poon

University of Kentucky Doctoral Dissertations

The studies presented in this work were completed with the goal ofgaining greater insight into the roles of protein oxidation in brain aging and age-relatedcognitive impairment. Aging is associated with the impairment of physiological systemssuch as the central nervous system (CNS), homeostatic system, immune system, etc.Functional impairments of the CNS is associated with increased susceptibility to developmany neurodegenerative diseases such as Alzheimer's diseases (AD), Parkinson's disease(PD), and amyotrophic lateral sclerosis (ALS). One of the most noticeable functionalimpairments of the CNS is manifested by cognitive decline. In the past three decades, thefree radical theory of aging has gained relatively strong support …

Effects Of A Restored Elk Population On Soils, Vegetation, And Water Quality In Eastern Kentucky, Julia M. Ter Beest

University of Kentucky Master's Theses

A restored elk (Cervus elaphus) population in eastern Kentucky may be affecting ecosystem processes in a landscape composed of reclaimed grassland expanses and isolated forest remnants. Elk routinely select forested ridge-tops as resting and ruminating sites. These locations are characterized by sparse or absent leaf litter, trampled and diminished vegetation, large deposits of dung, and urine-saturated soils. In fall 2003, a series of fenced ungulate exclosures were constructed; 8 on highly disturbed forested ridge-tops and 8 on reclaimed grasslands. Soil analyses measured % moisture, pH, total nitrogen, total carbon, ammonium, nitrate, phosphorus, and major extractable cations in 0-10 cm and …

Mediating Indigenous Identity: Video, Advocacy, And Knowledge In Oaxaca, Mexico, Laurel Catherine Smith

University of Kentucky Doctoral Dissertations

In the southern Mexican state of Oaxaca, many indigenous communities further their struggles for greater political and cultural autonomy by working with transnational non-governmental organizations (NGOs). Communication technology (what I call comtech) is increasingly vital to these intersecting socio-spatial relations of activism and advocacy. In this dissertation, I examine how comtech offer indigenous individuals and organizations with the means for visualizing their political-cultural agendas. Approaching the access and use of comtech, especially video technologies, as a partial and situated technoscience, I inquire into how and why these activities reconfigure the production and evaluation of authoritative knowledge about indigenous peoples, places, …

Pragmatism And The Politics Of Rewilding Nature: The Case Of Grizzly Bear Reintroduction In Idaho, John G. Hintz

University of Kentucky Doctoral Dissertations

In 1975, the US Fish and Wildlife Service listed the grizzly bear as a threatened species under the Endangered Species Act. Following the listing, a recovery plan was drafted in which the Bitterroot Ecosystem of central Idaho and extreme western Montana was one of six proposed grizzly bear recovery areas. It was the only one of the six, however, which did not contain a resident population of grizzlies. The Fish and Wildlife Service eventually accepted a proposal submitted by a coalition of environmental and timber industry groups. The coalition proposed to reestablish a population of grizzlies in the Bitterroot by …

The Use Of A Whole Genome Scan To Find A Genetic Marker For Degenerative Suspensory Ligament Desmitis In The Peruvian Paso Horse, Diane I. Strong

University of Kentucky Master's Theses

Degenerative suspensory ligament desmitis (DSLD) is a debilitating disease of connective tissues seen in many breeds but has become prevalent in the Peruvian Pasohorse. DSLD is believed to be a genetic disorder caused by one primary founder and most likely has a recessive mode of inheritance although a dominant or co-dominant mode of inheritance has not been ruled out.

A genome scan using 259 microsatellite markers was used to test for linkage disequilibrium between one or more markers and DSLD. Two groups of Peruvian Pasohorses were selected from one population including the US and Canada. The only difference between the …

The Implied Reader In The Hispanic Children's Literature Of The "Encuento" Series, Genny D. Ballard

University of Kentucky Doctoral Dissertations

This dissertation discusses the implied reader in the EnCuento series illustrated childrens stories. All the stories are written by well-known Hispanic authors. This work elucidates historical, cultural, and semiotic gaps in the reading process. It explores the ways in which textual elements- such as style, focalization, and manipulation of readers expectations - affect the implied readers ability to produce or extract meaning. Our study will add to knowledge of the function of the implied reader in childrens texts. This study is divided into four chapters, each focusing on the implied reader. Chapter 1 provides an introduction to the series discussing …

Optimization Of Machining Performance In Contour Finish Turning Operations, Masaya Hagiwara

University of Kentucky Master's Theses

Unlike straight turning, the effective cutting conditions and tool geometry in contour turning operations are changing with changing workpiece profile. This causes a wide variation in machining performance such as chip flow and chip breakability during the operation. This thesis presents a new methodology for optimizing the machining performance, namely, chip breakability and surface roughness in contour finish turning operations. First, a computer program to calculate the effective cutting conditions and tool geometry along the contour workpiece profile is developed. Second, a methodology to predict the chip side-flow for complex grooved tool inserts is formulated and integrated in the current …

Tsc2 Modulates Actin Cytoskeleton And Focal Adhesion Through Tsc1-Binding Domain And The Rac1 Gtpase, Elena Goncharova, Dmitry Goncharov, Daniel J. Noonan, Vera P Krymskaya

Molecular and Cellular Biochemistry Faculty Publications

Tuberous sclerosis complex (TSC) 1 and TSC2 are thought to be involved in protein translational regulation and cell growth, and loss of their function is a cause of TSC and lymphangioleiomyomatosis (LAM). However, TSC1 also activates Rho and regulates cell adhesion. We found that TSC2 modulates actin dynamics and cell adhesion and the TSC1-binding domain (TSC2-HBD) is essential for this function of TSC2. Expression of TSC2 or TSC2-HBD in TSC2-/- cells promoted Rac1 activation, inhibition of Rho, stress fiber disassembly, and focal adhesion remodeling. The down-regulation of TSC1 with TSC1 siRNA in TSC2-/- cells activated Rac1 and induced loss of …

Forage News [2004-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA in Owensboro
• Kentucky Alfalfa Conference – 25^TH Anniversary
• Groups Form National Alfalfa Coalition
• Eleven Seasons of Pasture Productivity and Heifer Performance Under Rotational Grazing: Data From WICST.
• Hay Storage Update
• Upcoming Events

Monitoring Heart Rate In Drosophila Larvae By Various Approaches, Sameera Dasari, Robin L. Cooper

Biology Faculty Publications

No abstract provided.

Monitoring Carbon Dioxide Production By Drosophila Larvae, Robin L. Cooper, D. Nicholas Mcletchie

Biology Faculty Publications

No abstract provided.

Evaluation Of Us 119 Pine Mountain Safety Improvements: Ihsdm Analysis Of Post Construction, Will Conkin, Nikiforos Stamatiadis

Kentucky Transportation Center Research Report

The spot improvements on US 119 in Letcher County between Whitesburg and Partridge were an attempt to improve safety throughout the corridor. To achieve this goal, the roadway alignment and cross section were changed at various locations. In addition, problems related to truck traffic were considered in redefining the roadway geometries. The total length of the spot improvement locations was approximately 6.9 miles.

Monitoring Activity Of Drosophila Larvae: Impedance & Video Microscopy Measures, Ann Simone Cooper, Robin L. Cooper

Biology Faculty Publications

No abstract provided.

Methods And Apparatus For In Vivo Identification And Characterization Of Vulnerable Atherosclerotic Plaques, Pedro Moreno, Robert A. Lodder, William O'Connor, James E. Muller

Internal Medicine Faculty Patents

Methods and apparatus for analyzing the chemical composition of vulnerable plaques with an intravascular catheter having a near-infrared light source, a fiber-optic probe, a mechanism for directing the light from the light source into a blood vessel, and detectors for detecting light reflected or scattered by the tissue. The light source may be a tunable laser, and may transmit an incident beam having a wavelength ranging from 1400 to 4100 nm. A computer may be included to receive and process the spectral data in the analysis of the vulnerable plaques. A catheter system may be configured to provide near-IR spectrometric …