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Cyclooxygenase-2 Mediates Microglial Activation And Secondary Dopaminergic Cell Death In The Mouse Mptp Model Of Parkinson's Disease, Rattanavijit Vijitruth, Mei Liu, Dong-Young Choi, Xuan V. Nguyen, Randy L. Hunter, Guoying Bing

Neuroscience Faculty Publications

BACKGROUND: Accumulating evidence suggests that inflammation plays an important role in the progression of Parkinson's disease (PD). Among many inflammatory factors found in the PD brain, cyclooxygenase (COX), specifically the inducible isoform, COX-2, is believed to be a critical enzyme in the inflammatory response. Induction of COX-2 is also found in an experimental model of PD produced by administration of 1-methy-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

METHOD: COX-2-deficient mice or C57BL/6 mice were treated with MPTP to investigate the effects of COX-2 deficiency or by using various doses of valdecoxib, a specific COX-2 inhibitor, which induces inhibition of COX-2 on dopaminergic neuronal toxicity and …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Limestone-Based Material for Arsenic Removal from Drinking Water, Chelsea Campbell and others, Dept of Chemistry, WKU
• Opportunistic Water Education, Amanda Abnee Gumbert, Extension Associate for Environmental and Natural Resources Issues, UK
• Ten-Year Solute Concentration Patterns in Two Streams of Contrasting Land-Use in Western Kentucky and Tennessee, Susan P. Hendricks, Hancock Biological Station, Murray State University
• Pathogen TMDL Development using Load Duration Curves for Two Stream Segments in Rockcastle County, Kentucky, Joseph M. Ferguson, Kentucky DOW
• Beargrass Creek Water Quality Tool and TMDLs, Ward Wilson and others, Tetra Tech
• Pathogen and Sediment Transport in Muddy Creek …

Session 1d: Monitoring, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• An Examination of Statewide Watershed Watch Project Monitoring Data for 2005 Using GIS, Ken Cooke, Kentucky Division of Water, Frankfort
• Taking Watershed Watch to the Next Level: Grabbing the Attention of Local Elected Officials, Ken Cooke, Kentucky Division of Water, H. David Gabbard, LFUCG, Lexington
• Water Quality Data from Citizen Monitoring in the Licking River Region Kentucky 1998-2005: Trends and Issues, M.F. Hult, Daniel Carter Beard Environmental Center, and B.C. Reeder, Center for Environmental Education, Morehead State University
• Water Quality in the Upper Licking River Basin 2003-2005, Brian Reeder and others, Dept of Biological and Environmental …

Session 1c: Superfund, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• The University of Kentucky Superfund Basic Research Program: Overview and Examples of Research Projects, Bernhard Hennig, Leonidas Bachas, and Lindell Ormsbee, UK
• Reductive Dechlorination of Toxic Organics by Bimetallic Nanoparticles in Polyacrylic Acid (PAA) Functionalized MF Membranes, Jian Xu and Dibakar Bhattacharyya, Dept Chemical and Materials Engineering, UK
• Chlorinated Organic Compounds Destruction by Modified Fenton Reaction Involving Immobilized Iron-Chelate, YongChao Li, Leonidas Bachas, and Dibakar Bhattacharyya, Dept Chemical and Materials Engineering, UK
• An Outreach Program Translates Basic Research for Superfund Communities to Improve Health through Nutrition, Lisa Gaetke and Sandra Bastin, Dept Nutrition and Food Science, …

Session 1b: Groundwater, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• A Brief History and Current State of Groundwater Protection in Kentucky, James Webb, Beverly Oliver, and Peter Goodmann, Kentucky DOW, Groundwater Branch
• Summary of Groundwater Quality Data in the Jackson Purchase Region, Kentucky, E.G. Beck, J.S. Dinger, and P.C. Inkenbrandt, KGS
• Groundwater-Quality Assessment and Shallow Aquifer Model of Calloway County, Kentucky, P.C. Inkenbrandt, E.G. Beck, and J.S. Dinger, KGS
• Suspended Sediment and Pathogen Transport in Two Inner Bluegrass Karst Ground-Water Basins, A.E. Fryar and others, Dept Earth and Environmental Sciences, UK
• Locating and Mapping Domestic Water Wells in Marshall County, Kentucky, Wendy D. Langhi, Western …

Proceedings Of 2006 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was funded in part by the U.S. Environmental Protection Agency, with Clean Water Act, Section 319(h) grant money through the Kentucky Division of Water and the Kentucky Waterways Alliance, #C9994861-00.

Planning for this conference was conducted as part of the state water resources research annual program with the support and collaboration of the Department of Interior, U.S. Geological Survey and the University of Kentucky Research Foundation, under Grant Agreement No. 01HQGR0133.

The views and conclusions contained in this document are those of the abstract authors and should not be interpreted as necessarily representing the official policies, either expressed …

Evaluation Of Work Zone Safety Operations And Issues, Jerry G. Pigman, Kenneth R. Agent, Eric R. Green

Kentucky Transportation Center Research Report

Work zone activities are one of the areas with a high potential for compromised safety for workers and road users. Various measures have been taken to increase the level of safety in work zones; including the use of double fines for speeding and the application of speed displays based on radar detection. There has been an effort to improve the effectiveness and safety of flaggers by requiring the use of a stop/slow paddle for most work zone conditions, using lights on the paddle, and increasing visibility by using more reflective clothing. It has been suggested that safety in work zones …

Why Dairy Farmers Need And Want High Quality Alfalfa Hay: Quality Of Hay = Performance And Profitability, Donna M. Amaral-Phillips

Kentucky Alfalfa and Stored Forage Conference

Why do dairy farmers want high quality forages, including alfalfa hay, to feed to their milking herds? Feeding high-quality forages results in greater feed intake and as a result, dairy cows produce more milk, and they often times can produce this milk more economically. With advancing stage of plant maturity, fiber digestibility and protein content of the alfalfa plant decreases while the amount of fiber increases. Consequently, less energy is available to the cow when it consumes more mature alfalfa. Energy is the nutrient that most often limits performance in dairy or beef cattle - not protein. The take home …

Alfalfa Hay For Horses, Robert Coleman

Kentucky Alfalfa and Stored Forage Conference

When horse owners consider hay for their animals a number of criteria are generally considered. Of major importance is the hay must be free of mold and dust, it needs to contain nutrients needed by the horse and it must be palatable. If these criteria are met, the type of hay should not matter. However, that is not the case with many horse owners. When discussing hay, many owners first consider the type of hay. Can it be alfalfa or should it be a grass or how about a mix of grass and a legume. If a group of horse …

Interpreting Forage Quality Test Reports, Donna M. Amaral-Phillips

Kentucky Alfalfa and Stored Forage Conference

Forages are the foundation for building diets for beef and dairy cattle, horses, sheep and goats. The quality of these forages directly impacts forage intake, animal performance, and, ultimately, the profitability to their owners. As the quality of forages decline, consumption of that forage decreases and the amount of grain or byproducts which must be fed increases. The cost for a unit of performance increases and/or the animal can not perform (i.e. milk or gain) to the optimum level thus lowering profitability to the animal owner. These relationships are especially true with young and high performance ruminants, such as high …

Alfalfa As A Grazing Crop, Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

Over the past decade we have had several people address “Alfalfa as a Grazing Crop” including Warren Thompson, Jim Moutray, Gary Bates, Ken Johnson, Jason Sandefur, Byron Sleugh, and myself. Each has done an excellent job discussing the merits of this remarkable crop and the opportunities it offers for grazing. At the end of the Conference last year, I (as I always do) asked for your suggestions concerning topics for this years’ conference. As it has been for the past decade, “Grazing” was the most requested topic. In today’s presentation, let’s go back and revisit the question, “Is Grazing Alfalfa …

Principles And Practices Of Successful Alfalfa Establishment, Gary Bates

Kentucky Alfalfa and Stored Forage Conference

Alfalfa is the most widely known forage crop, due to its high quality and the versatility of use. There are few forages that are as widely distributed as alfalfa, and none that can produce the high yields and high quality. Even with all of the positives of alfalfa, growing this crop profitably is often difficult. An evaluation of alfalfa budgets shows that a key factor in the success with alfalfa is the stand life. Because of the establishment expense, an extra year or two of production can increase the lucrativeness of the stand. The start of having a long life …

Cbfbeta Is A Facultative Runx Partner In The Sea Urchin Embryo, Anthony J. Robertson, Carrie Dickey-Sims, Andrew Ransick, Dawn E. Rupp, John J. Mccarthy, James A. Coffman

Physiology Faculty Publications

BACKGROUND: Runx proteins are developmentally important metazoan transcription factors that form a heterodimeric complex with the non-homologous protein Core Binding Factor beta (CBFbeta). CBFbeta allosterically enhances Runx DNA binding but does not bind DNA itself. We report the initial characterization of SpCBFbeta, the heterodimeric partner of SpRunt-1 from the sea urchin Stronylocentrotus purpuratus.

RESULTS: SpCBFbeta is remarkably similar to its mammalian homologues, and like them it enhances the DNA binding of the Runt domain. SpCBFbeta is entirely of zygotic provenance and its expression is similar that of SpRunt-1, accumulating globally at late blastula stage then later localizing to endoderm and …

H2-M3-Restricted Cd8+ T Cells Are Not Required For Mhc Class Ib-Restricted Immunity Against Listeria Monocytogenes, Sarah E. F. D'Orazio, Christine A. Shaw, Michael N. Starnbach

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Studies using major histocompatibility complex (MHC)-Ia–deficient mice have shown that MHC-Ib–restricted CD8⁺ T cells can clear infections caused by intracellular pathogens such as Listeria monocytogenes. M3-restricted CD8⁺ T cells, which recognize short hydrophobic N-formylated peptides, appear to comprise a substantial portion of the MHC-Ib–restricted T cell response in the mouse model of L. monocytogenes infection. In this study, we isolated formyltransferase (fmt) mutant strains of L. monocytogenes that lacked the ability to add formyl groups to nascent polypeptides. These fmt mutant Listeria strains did not produce antigens that could be recognized by M3-restricted T …

Loss Of Sparc-Mediated Vegfr-1 Suppression After Injury Reveals A Novel Antiangiogenic Activity Of Vegf-A, Miho Nozaki, Eiji Sakurai, Brian J. Raisler, Judit Z. Baffi, Jassir Witta, Yuichiro Ogura, Rolf A. Brekken, E Helene Sage, Balamurali K. Ambati, Jayakrishna Ambati

Ophthalmology and Visual Science Faculty Publications

VEGF-A promotes angiogenesis in many tissues. Here we report that choroidal neovascularization (CNV) incited by injury was increased by excess VEGF-A before injury but was suppressed by VEGF-A after injury. This unorthodox antiangiogenic effect was mediated via VEGFR-1 activation and VEGFR-2 deactivation, the latter via Src homology domain 2-containing (SH2-containing) tyrosine phosphatase-1 (SHP-1). The VEGFR-1-specific ligand placental growth factor-1 (PlGF-1), but not VEGF-E, which selectively binds VEGFR-2, mimicked these responses. Excess VEGF-A increased CNV before injury because VEGFR-1 activation was silenced by secreted protein, acidic and rich in cysteine (SPARC). The transient decline of SPARC after injury revealed a temporal …

Forage News [2006-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA
• Heart of America Grazing Conference
• Nitrogen Costs and the Value of Legumes
• It Will Soon Be Calving Season
• UK Beef/Forage Field Day June 15
• Tall Fescue On-Line Monograph" Availability
• Comparative Lamb and Heifer Growth Rates on Non-Toxic and Endophyte-Free Tall Fescue
• Field Experience with Strip Grazing Standing Corn as an Economical Alternative for Wintering Beef Cows in the Midwest
• Free Nitrogen
• Upcoming Events

Perennial Warm Season Grasses In Grazing Programs, Mark Kennedy

Kentucky Grazing Conference

Many warm season perennial grasses were once an important part of the plant community in much of the Midwest. Conversion to cropping systems, overgrazing, lack of regular fire and increased competition from cool-season grasses and legumes have caused many of these grasses to disappear from much of the region. However, warm season grasses can compliment cool-season pastures if managed properly. Midwest stockmen are rediscovering the usefulness of warm season grasses in their overall forage program. Adding these grasses to forage systems has resulted in increased gains and improved livestock performance during the summer months when cool-season grasses are at their …

Non-Traditional Forages For Grazing: Turnips And Other Brassicas, Dave Robison

Kentucky Grazing Conference

Over the past few years there have been a number of production livestock producers that have realized the benefits of grazing turnips and other brassicas. Turnips and other brassicas have traditionally been utilized for late season grazing to help extend the grazing season. But other ways are being found to utilize these highly productive, high quality forage crops. My purpose in this presentation is to introduce the different types of turnips and brassicas and explain how they can be utilized for various systems and different uses.

Corn And Other Annuals, Jeff Mccutcheon

Kentucky Grazing Conference

There are many factors to consider when planting alternative crops. One of the most important factors is deciding if you need additional forage. In our area the basis of any grazing system should be perennial cool-season grasses and legumes. Have you done all that you can to improve that forage production through managed grazing and fertility management? If you have, then you may be ready to consider annual forages in your grazing system.

How I Use My Pastures, Doug Gehner, Russell C. Hackley, Gil Myers

Kentucky Grazing Conference

No abstract provided.

Tall Fescue From 1931-2006, Garry D. Lacefield

Kentucky Grazing Conference

Tall fescue is the most important coolseason grass grown in the “Heart of America”. It is a versatile plant used for animal feed, lawns and turf, and conservation purposes.

Mineral Supplements And Feed Additives--Can They Eliminate Fescue Toxicity?, John Thomas Johns

Kentucky Grazing Conference

Fescue is the predominate cool season grass in much of the United States due to its wide adaptation and hardiness. Many of these acres however, contain an endophytic fungus resulting in production of toxins with negative effects on grazing cattle. The two primary responses in beef cattle are an increase in core body temperature and a decrease in dry matter intake. As a result, decreases in reproductive rates, milk production, weaning weights and ADG are seen (Tables 1 and 2). Many approaches to pasture management have been advocated to overcome or minimize the problem such as incorporation of legumes, rotational …

Developing Watering Systems For Efficient Grazing, Kevin Laurent

Kentucky Grazing Conference

Whether you call it rotational grazing, intensive grazing or management intensive grazing, the economic benefits of controlling how and where your cattle graze are well documented. Increased forage utilization, greater stocking rates, greater legume persistence, reduced hay feeding and more uniform nutrient recycling are just some of the many benefits producers can take advantage of when practicing some form of controlled grazing. However, one of the greatest challenges to implementing a controlled grazing system is the delivery of stock water to the grazing animal.

Grazing Programs: Environmentally Friendly, Economically Sound And Agronomically Feasible, Sid Brantly

Kentucky Grazing Conference

The environmental soundness of our farms is the greatest, long term asset that farmers have. The topsoil, the trees, the cattle, the grass and crops on your farm are like unto a great financial account...and you are the banker. You make the decisions to: A) Use it all up now, B) Lock it away for someone in the future to make the decisions, or C) Utilize wisely in such a way that it is managed for financial success today, and still builds momentum for your future as well as your descendents.

Animal Behavior: Impacts On Grazing, Mark Kennedy

Kentucky Grazing Conference

I am not an expert on this subject and give all credit for my limited understanding of this subject to Dr. Fred Provenza, Utah State University; Katy Voth, Livestock for Landscapes, LLC; Jim Gerrish and others. I have tried to put into practice, both personally and with other producers, many of these principles to aid in improved grazing management. This presentation will address only 2 aspects of animal behavior; 1) How animal behavior impacts grazing distribution and forage utilization over the landscape, and 2) How animal behavior affects diet selection.

Growing Forage Crops For Wildlife, Don Ball

Kentucky Grazing Conference

Numerous forage crops adapted in the Southeast offer benefits in production of various domestic animals including beef and dairy cattle, horses, and sheep (Ball, et al., 2002). Many of the same attributes these plants offer when grown in connection with livestock enterprises are also valuable in wildlife settings. Furthermore, the attitudes of many wildlife managers are changing, and their desire for knowledge of forage crops is on the rise. Consequently, forage crops are becoming more important and more greatly appreciated by wildlife enthusiasts within our region.

Maximizing Production Of Beef Cattle On Pastures, Justin Sexten

Kentucky Grazing Conference

Standardized performance analysis (SPA) of beef cattle operations continues to demonstrate the importance of reducing feed related costs. As producers look for alternative methods to reduce feed costs the benefits of improved forage management become increasingly important due to the opportunity to reduce feed costs while improving animal performance. One note of caution related to maximizing beef production from pasture, maximizing anything must be done carefully, the difference between maximum success and a total disaster is a fine line.

Grazing Program For Goats, Greg Brann

Kentucky Grazing Conference

Goats are often referred to as four legged bush hogs and actually they perform best when consuming browse. Goats eat from the top down so fields have a mown appearance even when forage is three feet tall. The typical browse height for goats is up to four foot however, they will rise up on their hind legs and sometimes prop on others backs to reach higher. Goats have an affinity for high quality forage. Much of the vegetation we have considered weeds as beef producers is excellent forage for goats. Goats select the high quality leaves and tender growing tips …

From Traditional Confinement Dairying To Grazing Replacement Heifers, Bill Payne

Kentucky Grazing Conference

I would like to share an account of a transition from a conventional dairy operation to our current Management Intensive Grazing (MIG) enterprise. In 1974, I joined my father who at that time had been dairying on a 265 acre farm in Lincoln County, Kentucky for twenty five years. We fed our registered Holsteins corn silage and alfalfa haylage and purchased a manufactured feed. Our herd of 70 Holsteins spent most of their time on concrete. We did make an effort to allow access to an exercise lot when weather permitted. However, during the greater part of the 1990’s we …

Pastures For Horses: Challenges And Opportunities, Robert Coleman

Kentucky Grazing Conference

Horses were born to eat grass and how they evolved has allowed them to be efficient users of a high forage diet. In fact, for many of today’s horses, they will survive and thrive on an all forage diet.