Digital Commons Network^™

Monitoring Heart Function In Larval Drosophila Melanogaster For Physiological Studies, Ann S. Cooper, Kylah E. Rymond, Matthew A. Ward, Easter L. Bocook, Robin L. Cooper

Biology Faculty Publications

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained and restrained whole larvae For direct control of the environment around the heart another approach utilizes the dissected larvae and removal of the internal organs in order to bathe the heart in desired compounds. The exposed heart also allows membrane potentials to be monitored which can give insight of the ionic currents generated by the myocytes and for electrical conduction along the heart tube. These approaches have various advantages and disadvantages for future experiments that are discussed. …

Interactions Between Connected Half-Sarcomeres Produce Emergent Mechanical Behavior In A Mathematical Model Of Muscle, Kenneth S. Campbell

Physiology Faculty Publications

Most reductionist theories of muscle attribute a fiber's mechanical properties to the scaled behavior of a single half-sarcomere. Mathematical models of this type can explain many of the known mechanical properties of muscle but have to incorporate a passive mechanical component that becomes approximately 300% stiffer in activating conditions to reproduce the force response elicited by stretching a fast mammalian muscle fiber. The available experimental data suggests that titin filaments, which are the mostly likely source of the passive component, become at most approximately 30% stiffer in saturating Ca2+ solutions. The work described in this manuscript used computer modeling to …

Historical View And Physiology Demonstration At The Nmj Of The Crayfish Opener Muscle, Ann S. Cooper, Robin L. Cooper

Biology Faculty Publications

Here we present some of the key important discoveries made with the opener neuromuscular (NMJ) preparation of crustaceans and illustrate that there is still much to learn from this model preparation. In understanding the history one can appreciate why even today this NMJ still offers a rich playground to address questions regarding pre- and post-synaptic function and plasticity. The viability and ease of access to the terminal for intracellular as well as extracellular electrophysiology and imaging are significant advantages. The mechanisms behind the modulation of vesicular kinetics and fusion within the high- and low-output terminals are begging for investigation. The …

Forage News [2009-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA
• 10th Kentucky Grazing Conference
• KFGC Presents Awards
• KFGC Update
• Caldwell Willig is New Kentucky Forage Spokesman
• Forage Testing A Must for This Years' Hay Crop
• "Growing Alfalfa in the South" & "Understanding Forage Quality" New Available on NAF Website
• It's the Pitts: A Can of Worms
• Estimating Clover Percentage in Your Pasture
• Upcoming Events

The Impact Of Exercise Training Compared To Caloric Restriction On Hepatic And Peripheral Insulin Resistance In Obesity, Robert H. Coker, Rick H. Williams, Sophie E. Yeo, Patrick M. Kortebein, Don L. Bodenner, Philip A. Kern, William J. Evans

Clinical and Translational Science Faculty Publications

Context: It has been difficult to distinguish the independent effects of caloric restriction versus exercise training on insulin resistance.

Objective: Utilizing metabolic feeding and supervised exercise training, we examined the influence of caloric restriction vs. exercise training with and without weight loss on hepatic and peripheral insulin resistance.

Design, Participants, and Intervention: Thirty-four obese, older subjects were randomized to: caloric restriction with weight loss (CR), exercise training with weight loss (EWL), exercise training without weight loss (EX), or controls. Based on an equivalent caloric deficit in EWL and CR, we induced matched weight loss. Subjects in the EX group received …

Analog To Digital: Photography Copyright Then And Now, Kopana Terry

Library Presentations

No abstract provided.

Forage Spokesman Information, Tom Flowers, Vince Rawe, Caldwell Willig

Kentucky Grazing Conference

No abstract provided.

Animal Welfare--Animal Rights: Role Of Grazing, Roy Burris

Kentucky Grazing Conference

The care and handling of farm animals has drawn a lot of media attention lately. Some well-publicized events have given our industry the proverbial black-eye and led to a chain of events that has left livestock producers feeling very vulnerable to negative attacks. Although these events have been very isolated, they have had some very farreaching effects. Could they have been prevented? How? What do we do now?

Extended Grazing And Reduced Stored Feed, Edward N. Ballard

Kentucky Grazing Conference

Feed costs represent the major cost in most livestock production systems. A recently completed analysis of 225 Standardized Performance Analysis (SPA) Beef Cow Records on herds in Illinois and Iowa showed that feed cost was the overriding factor determining profitability, explaining over 57 percent of the herd-to-herd variation. Typically the cost of supplying nutrients to ruminant livestock is much greater using harvested feedstuffs as opposed to grazing pastures or crop residues. The primary function of a grassland farm is to convert solar energy to marketable livestock products in the most efficient manner. The fewer steps between the animal product and …

Stored Feed Needs: How Much? What Quality?, Jeff Lehmkuhler

Kentucky Grazing Conference

Feed resources are the largest variable expense in beef production systems outside the initial investment in the livestock. When we look at Standardized Performance Analysis (SPA) information from Texas and Minnesota, states that are at the extreme from an environment stance, the projected costs for maintaining a beef cow are strikingly similar. It is important to note that these costs include all expenses and opportunity costs. Getting back to the SPA information, in 2007 the annual cost to maintain a cow in Texas was determined to be roughly $590 while the Minnesota value was near $610 or only about $20/cow …

Tall Fescue Grazing Research, Glen Aiken

Kentucky Grazing Conference

‘Kentucky-31’ tall fescue, like it or hate it, covers 5 million acres of Kentucky and if you do not have it on your farm, it is likely that one of your bordering neighbors does! Tall fescue owes its persistence and productivity in large part to a fungal endophyte that infects most plants. This endophyte produces an array of alkaloids that benefit the plant in tolerating heat, drought, and grazing. Unfortunately, the endophyte also produces ergot alkaloids that can cause “fescue toxicosis” in cattle. Cattle inflicted with toxicosis have reduced dry matter intake, elevated body temperature, and often retain their winter …

Weed Control Options For Pastures, Jon Doran

Kentucky Grazing Conference

What is a weed? A pasture or hayfield “weed” is any plant that you deem as being out of place and/or a plant that is decreasing your desired forage production. Weeds may be classified in several ways including broadleaf weeds, woody plants, and undesirable grasses. Besides decreasing forage, weeds can also interfere with forage utilization, grazing distribution, etc. There are several methods available to control weeds and increase production of and access to desirable forages. The three main methods are cultural, mechanical, and chemical. The cultural method is quite simple-a thriving grass stand is an excellent form of weed control …

Our Experiences With Eastern Gamagrass, Tommy R. Yankey

Kentucky Grazing Conference

As livestock producers try to reduce their cost of production, many look at ways to reduce their feed costs. Feed costs have been identified as the largest single cost of livestock production, making up 50 to 70% of the total cost of production. Grazing is the cheapest way to feed cattle on a cost per pound of nutrient basis.

New Developments In Grazing Species And Varieties, S. Ray Smith

Kentucky Grazing Conference

During the last 10 years there have been several new forage species that have emerged as useful in grazing systems and many new varieties have been released of traditional species. Plant breeders continue to make improvement in our traditional pasture species like tall fescue, orchardgrass, and red and white clover. In the following paragraphs, I will focus more on some of the forage species that you may not be as familiar with and highlight the advancements occurring in developing new varieties for grazing. There are many sources of information on this subject. I especially encourage you to read the new …

Why Should I Improve My Grazing Program?, Garry D. Lacefield

Kentucky Grazing Conference

Grazing represents the cheapest way to feed ruminants on a cost per pound of nutrient basis. Stored feed is usually the single largest item in livestock budgets and cost or amount of stored feed is usually the best prediction of potential profitability in most beef cattle operations.

Foreword And Kfgc Award Winners [2009], Garry D. Lacefield, Christi L. Forsythe

Kentucky Grazing Conference

No abstract provided.

Genetic Insulator For Preventing Influence By Another Gene Promoter, Susheng Gan, Mingtang Xie

Plant and Soil Sciences Faculty Patents

A 16 bp polynucleotide sequence of Arabidopsis thaliana is a genetic insulator that can effectively isolate a transgene from positional effects of neighboring gene activities in transgenic plant cells.

An Eiav Field Isolate Reveals Much Higher Levels Of Subtype Variability Than Currently Reported For The Equine Lentivirus Family, Jodi K. Craigo, Shannon Barnes, Baoshan Zhang, Sheila J. Cook, Laryssa Howe, Charles J. Issel, Ronald C. Montelaro

Veterinary Science Faculty Publications

BACKGROUND: Equine infectious anemia virus (EIAV), a lentivirus that infects horses, has been utilized as an animal model for the study of HIV. Furthermore, the disease associated with the equine lentivirus poses a significant challenge to veterinary medicine around the world. As with all lentiviruses, EIAV has been shown to have a high propensity for genomic sequence and antigenic variation, especially in its envelope (Env) proteins. Recent studies have demonstrated Env variation to be a major determinant of vaccine efficacy, emphasizing the importance of defining natural variation among field isolates of EIAV. To date, however, published EIAV sequences have been …

The Leishmania Donovani Lipophosphoglycan Excludes The Vesicular Proton-Atpase From Phagosomes By Impairing The Recruitment Of Synaptotagmin V, Adrien F. Vinet, Mitsunori Fukuda, Salvatore J. Turco, Albert Descoteaux

Molecular and Cellular Biochemistry Faculty Publications

We recently showed that the exocytosis regulator Synaptotagmin (Syt) V is recruited to the nascent phagosome and remains associated throughout the maturation process. In this study, we investigated the possibility that Syt V plays a role in regulating interactions between the phagosome and the endocytic organelles. Silencing of Syt V by RNA interference revealed that Syt V contributes to phagolysosome biogenesis by regulating the acquisition of cathepsin D and the vesicular proton-ATPase. In contrast, recruitment of cathepsin B, the early endosomal marker EEA1 and the lysosomal marker LAMP1 to phagosomes was normal in the absence of Syt V. As Leishmania …

Measures Of Heart And Ventilatory Rates In Freely Moving Crayfish, Sonya M. Bierbower, Robin L. Cooper

Biology Faculty Publications

The fear, flight or fight response serves as the fundamental physiological basis for examining an organism's awareness of its environment under an impending predator attack. Although it is not known whether invertebrates posses an autonomic nervous system identical to that of vertebrates, evidence shows invertebrates have a sympathetic-like response to regulate the internal environment and ready the organism to act behaviorally to a given stimuli. Furthermore, this physiological response can be feasibly measured and it acts as a biological index for the animal's internal state. Measurements of the physiological response can be directly related to internal and external stressors through …

Parallel Metatranscriptome Analyses Of Host And Symbiont Gene Expression In The Gut Of The Termite Reticulitermes Flavipes, Aurélien Tartar, Marsha M. Wheeler, Xuguo Zhou, Monique R. Coy, Drion G. Boucias, Michael E. Scharf

Entomology Faculty Publications

BACKGROUND: Termite lignocellulose digestion is achieved through a collaboration of host plus prokaryotic and eukaryotic symbionts. In the present work, we took a combined host and symbiont metatranscriptomic approach for investigating the digestive contributions of host and symbiont in the lower termite Reticulitermes flavipes. Our approach consisted of parallel high-throughput sequencing from (i) a host gut cDNA library and (ii) a hindgut symbiont cDNA library. Subsequently, we undertook functional analyses of newly identified phenoloxidases with potential importance as pretreatment enzymes in industrial lignocellulose processing.

RESULTS: Over 10,000 expressed sequence tags (ESTs) were sequenced from the 2 libraries that aligned into …

Inhibition Of Rna Recruitment And Replication Of An Rna Virus By Acridine Derivatives With Known Anti-Prion Activities, Zsuzsanna Sasvari, Stéphane Bach, Marc Blondel, Peter D. Nagy

Plant Pathology Faculty Publications

BACKGROUND: Small molecule inhibitors of RNA virus replication are potent antiviral drugs and useful to dissect selected steps in the replication process. To identify antiviral compounds against Tomato bushy stunt virus (TBSV), a model positive stranded RNA virus, we tested acridine derivatives, such as chlorpromazine (CPZ) and quinacrine (QC), which are active against prion-based diseases.

METHODOLOGY/PRINCIPAL FINDINGS: Here, we report that CPZ and QC compounds inhibited TBSV RNA accumulation in plants and in protoplasts. In vitro assays revealed that the inhibitory effects of these compounds were manifested at different steps of TBSV replication. QC was shown to have an effect …

Forage News [2009-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 10th Kentucky Grazing Conference
• 30th "Anniversary" Kentucky Alfalfa Conference
• KFGC Update
• Growing Alfalfa in the South
• Effects of a Freeze on Forages
• Grazing and Eliminative Behavior of Horses Grazing Bermudagrass
• Spatial Distribution of Livestock Concentration Areas and Soil Nutrients in Pastures
• Effect if Humate Application on the Forage Yield and Quality of Bermudagrass and Tall Fescue
• Effect of Repeated or One-Time Grazing of Kudzu by Sheep and Goats in Central Georgia
• Upcoming Events

Market Situation And Outlook For Hogs, Poultry And Small Ruminants, A. Lee Meyer

Agricultural Economics Presentations

No abstract provided.

Beef, Dairy, And Equine, Kenneth H. Burdine

Agricultural Economics Presentations

No abstract provided.

Methods For Cancer Detection, Kimberly W. Anderson, Kimberly M.L. May, Leonidas G. Bachas

Chemical and Materials Engineering Faculty Patents

A method is provided for detecting an analyte indicative of a cancer or a metastatic disease condition, which utilizes the ability of the analyte to induce permeability in a barrier. The method includes providing a biosensor having a barrier which is substantially impermeable to an ion, a permeable membrane which is selective for the ion, and a detector capable of detecting the ion. The biosensor is contacted with a sample including at least one of the ion and the analyte, wherein the analyte causes at least a portion of the ion to pass through the barrier and the membrane. Passage …

Methods And Animal Model For Analyzing Age-Related Macular Degeneration, Jayakrishna Ambati

Ophthalmology and Visual Science Faculty Patents

Methods for testing candidate drugs for treatment of age-related macular degeneration are provided. Ccl2-deficient, and Ccr2-deficient mice are used to determine the effect of candidate drugs and treatments on development of age-related macular degeneration. Also provided is a Ccl2-deficient, Ccr2-deficient dual knockout mouse, which is a useful animal model for age-related macular degeneration.

Compositions Comprising Human Immunodeficiency Virus Tat Adsorbed To The Surface Of Anionic Nanoparticles, Russell J. Mumper, Jerold Woodward, Zhengrong Cui, Avindra Nath

Pharmaceutical Sciences Faculty Patents

Non-denatured, recombinant human immunodeficiency virus (HIV) Tat that is free of bacterial RNA and endotoxin is employed in an anti-HIV vaccine. A process of producing the recombinant Tat protein includes steps for removing bacterial RNA from the recombinant Tat and for removing endotoxin from the recombinant Tat protein. A Tat-adsorbed nanoparticle formulation and method of making the same. A method of vaccinating against and/or treating HIV infection comprises administering to a subject in need of such vaccination or treatment an immune-response inducing effective amount of the recombinant Tat protein.

Transcriptional Profiling Differences For Articular Cartilage And Repair Tissue In Equine Joint Surface Lesions, Michael J. Mienaltowski, Liping Huang, David D. Frisbie, C. Wayne Mcilwraith, Arnold J. Stromberg, Arne C. Bathke, James N. Macleod

Veterinary Science Faculty Publications

BACKGROUND: Full-thickness articular cartilage lesions that reach to the subchondral bone yet are restricted to the chondral compartment usually fill with a fibrocartilage-like repair tissue which is structurally and biomechanically compromised relative to normal articular cartilage. The objective of this study was to evaluate transcriptional differences between chondrocytes of normal articular cartilage and repair tissue cells four months post-microfracture.

METHODS: Bilateral one-cm2 full-thickness defects were made in the articular surface of both distal femurs of four adult horses followed by subchondral microfracture. Four months postoperatively, repair tissue from the lesion site and grossly normal articular cartilage from within the same …

Forage News [2009-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Anderson County to Host Sept. 3 Forage Day
• Enroll in 2009 Kentucky Grazing School
• 10th Kentucky Grazing Conference
• KFGC Update
• Producers Can Receive Up to $45.00/Ton Matching Funds for Growing Biomass
• Forage Spokesman Nominations - Deadline Sept. 15
• Alfalfa Intensive Training Set for Indianapolis
• Performance and Physiology of Yearling Steers Grazing Toxic Tall Fescue as Influenced by Feeding Soybean Hulls and Implanting with Steroidal Hormones
• New Developments in Forage Varieties
• Extending the Grazing Season
• Use Goats to Make Money and Lower Costs
• Upcoming Events