Digital Commons Network^™

Consumed With (And By) Collecting: Museology As A Narrative Strategy, Gretchen E. Henderson

disClosure: A Journal of Social Theory

No abstract provided.

Celebrity Culture And The Rise Of The Ordinary: An Interview With Joshua Gamson, David Hoopes, Drew Heverin

disClosure: A Journal of Social Theory

No abstract provided.

Consuming And Maintaining Difference: American Fans Resisting The Globalization Of Japanese Pop Culture, Laura Beltz Imaoka

disClosure: A Journal of Social Theory

No abstract provided.

Much Ado About Mutton: An Interview With Deborah Gewertz And Frederick Errington, Rebecca Lane, Christine Smith

disClosure: A Journal of Social Theory

No abstract provided.

Care, Cure, And Control: A Politics Of Dietetics, Rick Dolphijn

disClosure: A Journal of Social Theory

No abstract provided.

Invited To The Slaughter In Šurany, Slovakia, Brendan F. R. Edwards

disClosure: A Journal of Social Theory

No abstract provided.

Commodifying My Culture: An “Appalachian” Reflects On Her Role In Sustaining A Limited Discourse Of Appalachia, Amanda Fickey

disClosure: A Journal of Social Theory

No abstract provided.

Carolus Linnaeus (Carl Von Linné), 1707-1778: The Swede Who Named Almost Everything, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

No abstract provided.

Omega-3 Fatty Acids: Potential Role In The Management Of Early Alzheimer's Disease, Gregory A. Jicha, William R. Markesbery

Sanders-Brown Center on Aging Faculty Publications

Omega-3 fatty acids are essential for brain growth and development. They play an important role throughout life, as critical modulators of neuronal function and regulation of oxidative stress mechanisms, in brain health and disease. Docosahexanoic acid (DHA), the major omega-3 fatty acid found in neurons, has taken on a central role as a target for therapeutic intervention in Alzheimer's disease (AD). A plethora of in vitro, animal model, and human data, gathered over the past decade, highlight the important role DHA may play in the development of a variety of neurological and psychiatric disorders, including AD. Cross sectional and prospective …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• River Basins of Kentucky, Dan Carey, Kentucky Geological Survey
• An Initial Prioritization Approach for Potential Agricultural Best Management Practice Implementation Based on Subwatershed Indicators and Expert Knowledge, Brian Lee and Corey Wilson, Dept Landscape Architecture, UK
• Locating Karst Conduits in Cane Run Watershed of Central Kentucky Using Electrical Resistivity Methods, Junfeng Zhu and others, Kentucky Geological Survey
• Identification of DNA Biomarkers for Determining Sources of Fecal Pollution in Water, Rick Fowler and others, WATERS Laboratory, WKU
• Integrating Participatory Communication and Structured Public Involvement Processes to Better Address Superfund Issues: The Paducah Gaseous Diffusion Plant Future State …

Plenary Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Evaluating the Impact of Hemlock Woolly Adelgid Invasion on Headwater Streams, Joshua Adkins and Lynne Reiske-Kinney, Dept of Entomology, UK
• Effects of Roundup^® Exposure on Behavior and Reproductive Function in a Pond-Breeding Salamander, Catherine Aubee and Howard Whiteman, Dept of Biological Sciences, Murray State University

Proceedings Of 2010 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This conference was planned and conducted as part of the state water resources research annual program with the support and collaboration of the Department of the Interior, U.S. Geological Survey and the University of Kentucky Research Foundation, under Grant Agreement Number 06HQGR0087.

The views and conclusions contained in this document and presented at the symposium are those of the abstract authors and presenters and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Government or other symposium organizers and sponsors.

Efficient Activation Of Reconstructed Rat Embryos By Cyclin-Dependent Kinase Inhibitors, Robin L. Webb, Kirk A. Findlay, Michael A. Green, Tina L. Beckett, M. Paul Murphy

Molecular and Cellular Biochemistry Faculty Publications

Background

Over the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its current state, the process of SCNT is very inefficient (<5% success rate), with several technical and biological hurdles hindering development. Yet, SCNT provides investigators with powerful advantages over other approaches, such as allowing for prescreening for the desired level of transgene expression and eliminating the excess production of undesirable wild-type animals. The rat plays a significant role in biomedical research, but SCNT has been problematic for this species. In this study, we address one aspect of the problem by evaluating methods of activation in artificially constructed rat embryos.

Principal Findings

We demonstrate that treatment with a calcium ionophore (ionomycin) combined with a variety of cyclin-dependent kinase inhibitors is an effective way to activate rat embryos. This is in contrast to methods developed for the mouse embryo, which tolerates much less specific chemical treatments. Methods developed to activate …

Dopamine Neuron Stimulating Actions Of A Gdnf Propeptide, Luke H. Bradley, Josh Fuqua, April Richardson, Jadwiga Turchan-Cholewo, Yi Ai, Kristen A. Kelps, John D. Glass, Xiuquan He, Zhiming Zhang, Richard Grondin, O. Meagan Littrell, Peter Huettl, Francois Pomerleau, Don M. Gash, Greg A. Gerhardt

Neuroscience Faculty Publications

BACKGROUND: Neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), have shown great promise for protection and restoration of damaged or dying dopamine neurons in animal models and in some Parkinson's disease (PD) clinical trials. However, the delivery of neurotrophic factors to the brain is difficult due to their large size and poor bio-distribution. In addition, developing more efficacious trophic factors is hampered by the difficulty of synthesis and structural modification. Small molecules with neurotrophic actions that are easy to synthesize and modify to improve bioavailability are needed.

METHODS AND FINDINGS: Here we present the neurobiological actions of dopamine …

Eicosapentaenoic Acid Preserves Diaphragm Force Generation Following Endotoxin Administration, Gerald S. Supinski, Jonas Vanags, Leigh Ann Callahan

Internal Medicine Faculty Publications

INTRODUCTION: Infections produce severe respiratory muscle weakness, which contributes to the development of respiratory failure. An effective, safe therapy to prevent respiratory muscle dysfunction in infected patients has not been defined. This study examined the effect of eicosapentaenoic acid (EPA), an immunomodulator that can be safely administered to patients, on diaphragm force generation following endotoxin administration.

METHODS: Rats were administered the following (n = 5/group): (a) saline, (b) endotoxin, 12 mg/kg IP, (c) endotoxin + EPA (1.0 g/kg/d), and (d) EPA alone. Diaphragms were removed and measurements made of the diaphragm force-frequency curve, calpain activation, caspase activation, and protein carbonyl …

A Monomeric Variant Of Insulin Degrading Enzyme (Ide) Loses Its Regulatory Properties, Eun Suk Song, David W. Rodgers, Louis B. Hersh

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: Insulin degrading enzyme (IDE) is a key enzyme in the metabolism of both insulin and amyloid beta peptides. IDE is unique in that it is subject to allosteric activation which is hypothesized to occur through an oligomeric structure.

METHODOLOGY/PRINCIPAL FINDINGS: IDE is known to exist as an equilibrium mixture of monomers, dimers, and higher oligomers, with the dimer being the predominant form. Based on the crystal structure of IDE we deleted the putative dimer interface in the C-terminal region, which resulted in a monomeric variant. Monomeric IDE retained enzymatic activity, however instead of the allosteric behavior seen with wild …

Molecular Evidence For A Functional Ecdysone Signaling System In Brugia Malayi, George Tzertzinis, Ana L. Egaña, Subba Reddy Palli, Marc Robinson-Rechavi, Chris R. Gissendanner, Canhui Liu, Thomas R. Unnasch, Claude V. Maina

Entomology Faculty Publications

BACKGROUND: Filarial nematodes, including Brugia malayi, the causative agent of lymphatic filariasis, undergo molting in both arthropod and mammalian hosts to complete their life cycles. An understanding of how these parasites cross developmental checkpoints may reveal potential targets for intervention. Pharmacological evidence suggests that ecdysteroids play a role in parasitic nematode molting and fertility although their specific function remains unknown. In insects, ecdysone triggers molting through the activation of the ecdysone receptor: a heterodimer of EcR (ecdysone receptor) and USP (Ultraspiracle).

METHODS AND FINDINGS: We report the cloning and characterization of a B. malayi EcR homologue (Bma-EcR). Bma-EcR dimerizes with …

Forage News [2010-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Alfalfa Conference Reflections After 30 Years
• Kentucky Alfalfa Awards
• Hay Winners
• UK Grazing Alfalfa DVD on YouTube
• Kentucky Grazing School April 14-15
• USDA Issues Final Rule on Organic Access to Pasture
• UK Forage Variety Test Available
• Points to Ponder

Kentucky River Watershed Watch: A Summary Of Volunteer Water Quality Sampling Efforts In The Kentucky River Basin From 1999 To 2009, Malissa Mcalister, Madhu Akasapu, Ben Albritton, Lindell Ormsbee, Dan Carey

Kentucky River Watershed Watch

No abstract provided.

A Conceptual Framework Of Information Requirements For Scientists Using Human Biological Samples, Sujin Kim

Information Science Faculty Publications

Introduction. This study was undertaken to develop an information requirement framework for scientists who use biological samples and related data in their research.

Method. A self-reporting questionnaire completed by 137 respondents was used to collect data regarding demographics, bio-sample management, bio-sample use and requirements, data requirements, and work and research-related roles and activities.

Analysis. Descriptive and TwoStep Cluster analyses were used to analyse the survey data necessary for developing a framework of information requirements.

Results. Two groups of biomedical scientists (clinical group and basic scientist group) were formed by their distinct characteristics. A conceptual framework of information requirements for bio-sample …

Growing Alfalfa For Wildlife, Don Ball

Kentucky Alfalfa and Stored Forage Conference

Alfalfa has long been recognized as a superb forage crop, which is why it is widely grown for dairy cattle, horses, sheep, and many other types of domesticated forage-consuming animals. Reasons for its popularity include wide adaptation, excellent nutritive value, good yield potential, perennial growth habit, a long growing season, and the fact that (in association with Rhizobium bacteria) it is a nitrogen-fixing legume that does not require periodic applications of nitrogen fertilizer.

Alfalfa Varieties For The Future, Joe Bouton

Kentucky Alfalfa and Stored Forage Conference

When breeding tomorrow’s alfalfa varieties, most groups employ a model of combining traditional plant breeding with biotechnology tools in order to incorporate useful traits. In this model, the conventional variety development process will be the method of choice for most traits where breeders have traditionally made progress such as adaptation, heading date, disease and insect resistance, general persistence conditions such as grazing and traffic tolerance, and even yield. It has been very successful in adding economic value to the forage and livestock operations of many producers (Bouton 2007). These traditionally developed varieties will also be used as the germplasm platform …

Alfalfa Hay For Horses, Robert Coleman

Kentucky Alfalfa and Stored Forage Conference

When horse owners consider hay for their animals, a number of criteria are generally considered. Of major importance is the hay must be free of mold and dust. It needs to contain nutrients needed by the horse and it must be palatable. If these criteria are met, the type of hay should not matter. However, that is not the case with many horse owners. When discussing the selection of hay, many owners first consider the type of hay. Can it be alfalfa or should it be a grass or how about a mix of grass and a legume. If a …

Value Of Alfalfa In Rotation, S. Ray Smith

Kentucky Alfalfa and Stored Forage Conference

There are about 23 million acres of alfalfa in the US. Alfalfa plus other hay is the most valuable crop in the US, behind only corn and soybeans. In Kentucky alfalfa is planted on over 300,000 acres and is an economically important crop for beef and dairy farmers, cash hay producers, and provides tremendous benefits for subsequent crops. There are also many non-agricultural benefits to alfalfa.

Microrna In Situ Hybridization In The Human Entorhinal And Transentorhinal Cortex, Peter T. Nelson, James Dimayuga, Bernard R. Wilfred

Pathology and Laboratory Medicine Faculty Publications

MicroRNAs (miRNAs) play key roles in gene expression regulation in both healthy and disease brains. To better understand those roles, it is necessary to characterize the miRNAs that are expressed in particular cell types under a range of conditions. In situ hybridization (ISH) can demonstrate cell- and lamina-specific patterns of miRNA expression that would be lost in tissue-level expression profiling. In the present study, ISH was performed with special focus on the human entorhinal cortex (EC) and transentorhinal cortex (TEC). The TEC is the area of the cerebral cortex that first develops neurofibrillary tangles in Alzheimer's disease (AD). However, the …

Mitochondrial Phylogenomics Of The Bivalvia (Mollusca): Searching For The Origin And Mitogenomic Correlates Of Doubly Uniparental Inheritance Of Mtdna, Hélène Doucet-Beaupré, Sophie Breton, Eric G. Chapman, Pierre U. Blier, Arthur E. Bogan, Donald T. Stewart, Walter R. Hoeh

Entomology Faculty Publications

BACKGROUND: Doubly uniparental inheritance (DUI) is an atypical system of animal mtDNA inheritance found only in some bivalves. Under DUI, maternally (F genome) and paternally (M genome) transmitted mtDNAs yield two distinct gender-associated mtDNA lineages. The oldest distinct M and F genomes are found in freshwater mussels (order Unionoida). Comparative analyses of unionoid mitochondrial genomes and a robust phylogenetic framework are necessary to elucidate the origin, function and molecular evolutionary consequences of DUI. Herein, F and M genomes from three unionoid species, Venustaconcha ellipsiformis, Pyganodon grandis and Quadrula quadrula have been sequenced. Comparative genomic analyses were carried out on these …

Forage News [2010-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Roundup Ready Alfalfa: Comment Period Deadline February 16, 2010
• 30th Anniversary Kentucky Alfalfa Conference
• Plan Now to Attend AGA Annual Conference, Feb. 4-7, Lexington
• Does Amount of "Hard" Alfalfa Seed Really Matter?
• What's Eating your Orchardgrass
• Forages at KCA
• Heart of America Grazing Conference
• Hay and Horses
• Grazing 101: Enroll in 2010 Kentucky Grazing School
• Clovers in Kentucky
• Commercial Hay Dryer Made in Kentucky

Anti-Argonaute Rip-Chip Shows That Mirna Transfections Alter Global Patterns Of Mrna Recruitment To Microribonucleoprotein Complexes, Wang-Xia Wang, Bernard R. Wilfred, Yanling Hu, Arnold J. Stromberg, Peter T. Nelson

Pathology and Laboratory Medicine Faculty Publications

MicroRNAs (miRNAs) play key roles in gene expression regulation by guiding Argonaute (AGO)-containing microribonucleoprotein (miRNP) effector complexes to target polynucleotides. There are still uncertainties about how miRNAs interact with mRNAs. Here we employed a biochemical approach to isolate AGO-containing miRNPs from human H4 tumor cells by co-immunoprecipitation (co-IP) with a previously described anti-AGO antibody. Co-immunoprecipitated (co-IPed) RNAs were subjected to downstream Affymetrix Human Gene 1.0 ST microarray analysis. During rigorous validation, the "RIP-Chip" assay identified target mRNAs specifically associated with AGO complexes. RIP-Chip was performed after transfecting brain-enriched miRNAs (miR-107, miR-124, miR-128, and miR-320) and nonphysiologic control miRNA to identify …

Methods To Impair Hematologic Cancer Progenitor Cells And Compounds Related Thereto, Craig Jordan

Pharmaceutical Sciences Faculty Patents

Primitive or progenitor hematologic cancer cells have been implicated in the early stages and development of leukemia and malignant lymphoproliferative disorders, including acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML) and chronic lymphoid leukemia (CLL). Interleukin-3 receptor alpha chain (IL-3Rα or CD123) is strongly expressed on progenitor hematologic cancer cells, but is virtually undetectable on normal bone marrow cells. The present invention provides methods of impairing progenitor hematologic cancer (e.g., leukemia and lymphomic) cells by selectively targeting cells expressing CD123. These methods are useful in the detection and treatment of leukemias and malignant lymphoproliferative disorders. Also provided are compounds useful …

Genomic Profiling Of Messenger Rnas And Micrornas Reveals Potential Mechanisms Of Tweak-Induced Skeletal Muscle Wasting In Mice, Siva K. Panguluri, Shephali Bhatnagar, Akhilesh Kumar, John J. Mccarthy, Apurva K. Srivastava, Nigel G. Cooper, Robert F. Lundy, Ashok Kumar

Physiology Faculty Publications

BACKGROUND: Skeletal muscle wasting is a devastating complication of several physiological and pathophysiological conditions. Inflammatory cytokines play an important role in the loss of skeletal muscle mass in various chronic diseases. We have recently reported that proinflammatory cytokine TWEAK is a major muscle-wasting cytokine. Emerging evidence suggests that gene expression is regulated not only at transcriptional level but also at post-transcriptional level through the expression of specific non-coding microRNAs (miRs) which can affect the stability and/or translation of target mRNA. However, the role of miRs in skeletal muscle wasting is unknown.

METHODOLOGY/PRINCIPAL FINDINGS: To understand the mechanism of action of …