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Stem Cells For The Treatment Of Neurodegenerative Diseases, Elise Dantuma, Stephanie Merchant, Kiminobu Sugaya Jan 2010

Stem Cells For The Treatment Of Neurodegenerative Diseases, Elise Dantuma, Stephanie Merchant, Kiminobu Sugaya

Faculty Bibliography 2010s

Stem cells off er an enormous pool of resources for the understanding of the human body. One proposed use of stem cells has been as an autologous therapy. The use of stem cells for neurodegenerative diseases has become of interest. Clinical applications of stem cells for Alzheimer disease, Parkinson disease, amyotrophic lateral sclerosis, and multiple sclerosis will increase in the coming years, and although great care will need to be taken when moving forward with prospective treatments, the application of stem cells is highly promising.

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Chloroplast-Derived Vaccine Antigens Confer Dual Immunity Against Cholera And Malaria By Oral Or Injectable Delivery, Abdoreza Davoodi-Semiromi, Melissa Schreiber, Samson Nalapalli, Dheeraj Verma, Nameirakpam D. Singh, Robert K. Banks, Debopam Chakrabarti, Hnery Daniell Jan 2010

Chloroplast-Derived Vaccine Antigens Confer Dual Immunity Against Cholera And Malaria By Oral Or Injectable Delivery, Abdoreza Davoodi-Semiromi, Melissa Schreiber, Samson Nalapalli, Dheeraj Verma, Nameirakpam D. Singh, Robert K. Banks, Debopam Chakrabarti, Hnery Daniell

Faculty Bibliography 2010s

Cholera and malaria are major diseases causing high mortality The only licensed cholera vaccine is expensive, immunity is lost in children within 3 years and adults are not fully protected No vaccine is yet available for malaria Therefore, in this study, the cholera toxin-B subunit (CTB) of Vibrio cholerae fused to malarial vaccine antigens apical membrane antigen-1 (AMA1) and merozoite surface protein-1 (MSP1) was expressed in lettuce and tobacco chloroplasts Southern blot analysis confirmed homoplasmy and stable integration of transgenes CTB-AMA1 and CTB-MSP1 fusion proteins accumulated up to 13 17% and 10 11% (total soluble protein, TSP) in tobacco and …

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Stat3-Mediated Metabolic Switch Is Involved In Tumour Transformation And Stat3 Addiction, Marco Demaria, Carlotta Giorgi, Magdalena Lebiedzinska, Giovanna Esposito, Luca D'Angeli, Antonietta Bartoli, Daniel J. Gough, James Turkson, David E. Levy, Christine J. Watson, Mariusz R. Wieckowski, Paolo Provero, Paolo Pinton, Valeria Poli Jan 2010

Stat3-Mediated Metabolic Switch Is Involved In Tumour Transformation And Stat3 Addiction, Marco Demaria, Carlotta Giorgi, Magdalena Lebiedzinska, Giovanna Esposito, Luca D'Angeli, Antonietta Bartoli, Daniel J. Gough, James Turkson, David E. Levy, Christine J. Watson, Mariusz R. Wieckowski, Paolo Provero, Paolo Pinton, Valeria Poli

Faculty Bibliography 2010s

The pro-oncogenic transcription factor STAT3 is constitutively activated in a wide variety of tumours that often become addicted to its activity, but no unifying view of a core function determining this widespread STAT3-dependence has yet emerged. We show here that constitutively active STAT3 acts as a master regulator of cell metabolism, inducing aerobic glycolysis and down-regulating mitochondrial activity both in primary fibroblasts and in STAT3-dependent tumour cell lines. As a result, cells are protected from apoptosis and senescence while becoming highly sensitive to glucose deprivation. We show that enhanced glycolysis is dependent on HIF-1a up-regulation, while reduced mitochondrial activity is …

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A Study Of Factors Contributing To Self-Reported Anomalies In Civil Aviation, Chris Andrzejczak Jan 2010

A Study Of Factors Contributing To Self-Reported Anomalies In Civil Aviation, Chris Andrzejczak

Electronic Theses and Dissertations

A study investigating what factors are present leading to pilots submitting voluntary anomaly reports regarding their flight performance was conducted. The study employed statistical methods, text mining, clustering, and dimensional reduction techniques in an effort to determine relationships between factors and anomalies. A review of the literature was conducted to determine what factors are contributing to these anomalous incidents, as well as what research exists on human error, its causes, and its management. Data from the NASA Aviation Safety Reporting System (ASRS) was analyzed using traditional statistical methods such as frequencies and multinomial logistic regression. Recently formalized approaches in text …

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Branched And Spiral Organic Nanotubes Based On The Self-Assembly Of Bile Acids, Xuejun Zhang Jan 2010

Branched And Spiral Organic Nanotubes Based On The Self-Assembly Of Bile Acids, Xuejun Zhang

Electronic Theses and Dissertations

The self-assembly of chiral amphiphilic molecules in aqueous solutions is of particular interest because the chirality of individual molecules is often expressed in their supermolecular structures. Self-assembled tubes made of chiral amphiphilic molecules represent useful supramolecular architectures which hold promise as controlled release vehicles for drug delivery, encapsulates for functional molecules, and nanoreactors for chemical reactions. Lithocholic acid (LCA) is a secondary bile acid with the concentration being identical to that of cholesterol in the hepatic bile and gallbladder. It has a rigid, nearly planar hydrophobic steroid nucleus, with four hydrogen atoms and one hydroxyl group directed toward the concave …

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Tuning The Properties Of Nanomaterials As Function Of Surface And Environment, Ajay Karakoti Jan 2010

Tuning The Properties Of Nanomaterials As Function Of Surface And Environment, Ajay Karakoti

Electronic Theses and Dissertations

Nanotechnology has shaped the research and development in various disciplines of science and technology by redefining the interdisciplinary research. It has put the materials science at the forefront of technology by allowing the researchers to engineer materials with properties ranging from electronics to biomedical by using materials as diverse as ceramics to just plain carbon. These exceptional properties are achieved by minimizing the dimension of particles in such smaller domains that the boundary between the individual atoms, ions or cluster of particles is very small. This results in a change in conventional properties of particles from continuum physics to quantum …

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Study Of The Interactions Of Proteins, Cells And Tissue With Biomaterials, Abhijeet Bhalkikar Jan 2010

Study Of The Interactions Of Proteins, Cells And Tissue With Biomaterials, Abhijeet Bhalkikar

Electronic Theses and Dissertations

Bioengineering is the application of engineering principles to address challenges in the fields of biology and medicine. Biomaterials play a major role in bioengineering. This work employs a three level approach to study the various interactions of biomaterials with proteins, cells and tissue in vitro. In the first study, we qualitatively and quantitatively analyzed the process of protein adsorption of two enzymes to two different surface chemistries, which are commonly used in the field. In the second study, we attempted to engineer a tissue construct to build a biocompatible interface between a titanium substrate and human skin. In the third …

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Mechanical Properties Of Carbon Nanotube/Metal Composites, Ying Sun Jan 2010

Mechanical Properties Of Carbon Nanotube/Metal Composites, Ying Sun

Electronic Theses and Dissertations

Carbon nanotubes (CNTs) have captured a great deal of attention worldwide since their discovery in 1991. CNTs are considered to be the stiffest and strongest material due to their perfect atomic arrangement and intrinsic strong in-plane sp 2—sp 2 covalent bonds between carbon atoms. In addition to mechanical properties, CNTs have also shown exceptional chemical, electrical and thermal properties. All these aspects make CNTs promising candidates in the development of novel multi-functional nanocomposites. Utilizing CNTs as fillers to develop advanced nanocomposites still remains a challenge, due to the lack of fundamental understanding of both material processing at the nanometer scale …

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Mitigating Misbehavior In Wireless Networks: A Game Theoretic Approach, Wenjing Wang Jan 2010

Mitigating Misbehavior In Wireless Networks: A Game Theoretic Approach, Wenjing Wang

Electronic Theses and Dissertations

In a distributed wireless system, multiple network nodes behave cooperatively towards a common goal. Though such assumptions on cooperation are desirable (e.g., controlling the transmit power level, reducing interference for each other, revealing private information, adhering to network policies) for analyzing and modeling, certain nodes belonging to a real-world system have often shown to deviate. These nodes, known as misbehaving nodes, bring more challenges to the design of the wireless network because the unreliable channel makes the actions of the nodes hidden from each other. In this dissertation, we analyze two types of misbehavior, namely, selfish noncooperation and malicious attacking. …

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Comparison Of Traditional Standard Drainfield With Innovative B&G Treatment Bed For Nutrient Removal From Septic Tank Wastewater, Fahim Hossain Jan 2010

Comparison Of Traditional Standard Drainfield With Innovative B&G Treatment Bed For Nutrient Removal From Septic Tank Wastewater, Fahim Hossain

Electronic Theses and Dissertations

Nowadays people are more alert about conservation of water and water scarcity. The amount of usable water is decreasing due to unavailability of pure water for day to day use. Both surface and groundwater is contaminated by untreated wastewater discharged from improper onsite wastewater treatment system, nutrient laden agricultural runoff and increasing use of fertilizer in fields. This elevated nutrient level is increasing the maintenance and operation cost of water treatment plant. So it is an important task to remove those nutrients from wastewater and other water bodies by applying environmental friendly process. In the USA, about 25% homes are …

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Control Of Hydrogen Sulfide Emissionsusing Autotrophic Denitrificationlandfill Biocovers, Daoroong Sungthong Jan 2010

Control Of Hydrogen Sulfide Emissionsusing Autotrophic Denitrificationlandfill Biocovers, Daoroong Sungthong

Electronic Theses and Dissertations

Hydrogen sulfide (H2S), a major odorous component emitted from construction and demolition debris landfills, has received increasing attention. Besides its unpleasant odor, long-term exposure to a very low concentration of H2S can cause a public health issue. Although cover materials such as soil and compost are recommended to be used routinely to control an odor problem from the landfills, the problem still remains. Autotrophic denitrification may have environmental applications including treatment of water, groundwater, wastewater or gaseous streams contaminated with sulfur and/or nitrogen compounds. However, there have been no studies reported in the literature on H2S removal using autotrophic denitrification …

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Assessment Of Aquifer Storage And Recovery Impact On Phosphorus Stability In Lake Sediment, Sha Liu Jan 2010

Assessment Of Aquifer Storage And Recovery Impact On Phosphorus Stability In Lake Sediment, Sha Liu

Electronic Theses and Dissertations

Lake Okeechobee, the second largest natural freshwater lake in the United States, had experienced a historical drought in 2007-2008 and the inflow to Lake Okeechobee has been reduced by 40% of the average daily mean between warm phase and cold phase due to the impact of Atlantic Multidecadal Oscillation in the past six decades. To cope with this water resources management problem, the US Army Corps of Engineers (USACE) proposed the largest national implementation plan of aquifer storage and recovery (ASR) project in the Kissimmee River Basin. Routine operation of ASR will deliver recovered water from ASR wells into the …

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Evaluation Of Oxidized Media Filtration Processes For The Treatment Of Hydrogen Sulfide In Groundwater, Vito Trupiano Jan 2010

Evaluation Of Oxidized Media Filtration Processes For The Treatment Of Hydrogen Sulfide In Groundwater, Vito Trupiano

Electronic Theses and Dissertations

This study evaluated alternative sulfide treatment processes for potable water systems that rely on groundwater supplies. Research for this study was conducted at the Imperial Lakes (IL) and Turner Road (TR) water treatment plants (WTPs) in Polk County, Florida. These WTPs are in the process of refurbishment and expansion, and will require the installation of a new groundwater well. The IL and TR WTPs both rely upon groundwater sources that contain total sulfide at concentrations ranging from 1.4 to 2.6 mg/L. Sulfide is a concern because if left untreated it can impact finished water quality, corrosivity, create undesirable taste and …

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A Study Of The Strength Of Pervious Pavement Systems, Ikenna Uju Jan 2010

A Study Of The Strength Of Pervious Pavement Systems, Ikenna Uju

Electronic Theses and Dissertations

This thesis presents a study on the strength properties of the different pervious pavement systems installed at the Stormwater Management Academy field laboratory at University of Central Florida (UCF), Orlando. The strength tests were performed both in the laboratory and in the field. Laboratory testing was conducted to determine the compressive strength and flexural strength of the various pavement surfaces. Evaluation of field pavement performance was performed by comparing the deflection basins using the Falling Weight Deflectometer test on pervious concrete and porous asphalt with conventional impervious concrete and asphalt pavements of similar layer profile and thickness, respectively. From literature …

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A Field Investigation For The Wind Load Performance Of Vegetated Greenroofs Using Monitoring Systems, Melike Minareci Jan 2010

A Field Investigation For The Wind Load Performance Of Vegetated Greenroofs Using Monitoring Systems, Melike Minareci

Electronic Theses and Dissertations

Greenroof systems have been shown to be an environmentally friendly alternative based on various factors; such as, reduced lifecycle cost, improved air quality, ambient temperature reduction, stormwater management credit, sustainability and preservation of the environment. Recent research studies attempt to determine the construction methods of an ideal greenroof for environmental purposes, yet there is an absence of standards for the best design required to achieve acceptable structural performance and sustainability under wind loads. As a result, there is a need to document the effectiveness of greenroofs under high wind events by addressing the following questions: Do winds have an effect …

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Parallel Adolescents, Tricia Windowmaker Jan 2010

Parallel Adolescents, Tricia Windowmaker

HIM 1990-2015

The general intent of my thesis is to write two novellas that show the differences in ways of life in two completely dissimilar states, and the conflicts that occurred therein. Therefore, the novellas will include a variation in gender, setting, and conflict. The main characters will be roughly the same age, but I will explore how the setting they live in has affected conflicts they have to deal with. I will explore writing these two novellas in the category of young adult fiction, as well as, first person narration for a close psychic distance.

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Ethnographic Reflection On Group Formation In Blizzard's "World Of Warcraft", John Christopher Spottke Jan 2010

Ethnographic Reflection On Group Formation In Blizzard's "World Of Warcraft", John Christopher Spottke

Electronic Theses and Dissertations

Cyberanthropology or the anthropology of cyber space/culture is an emerging subfield of cultural anthropology that deals with the varied integration of human beings and technology. This specialized area of study focuses on topics ranging from new technologies used in ethnographic research to information and communication technologies utilized by specific societal groups. Communication technology encompasses the World Wide Web, email, and online multiperson interactive spaces such as chatrooms and video games. In this work, I ethnographically investigate human social interactions in the online gaming realm of World of Warcraft. On the whole, the expanding numbers of virtual communities in existence today …

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Taxonomy Versus Phylogeny Phylogeography Of Marsh Rabbits Without Hopping To Conclusions, Rosanna M. Tursi Jan 2010

Taxonomy Versus Phylogeny Phylogeography Of Marsh Rabbits Without Hopping To Conclusions, Rosanna M. Tursi

Electronic Theses and Dissertations

Subspecific taxonomic designations solely based on morphological characters can often lead to erroneous assumptions about the evolutionary history of populations. This study sought to investigate evolutionary questions and conservation implications associated with morphological subspecific designations of island populations. To this end, I focused my attention on the Lower Keys of Florida, a unique chain of islands with well-known geologic history and rich in endemic, endangered subspecies. I employed genetic analyses to evaluate historical variation and contemporary restriction of gene flow between the endangered Lower Keys marsh rabbit (Sylvilagus palustris hefneri) and its sister mainland taxa. A Bayesian phylogeny using 1063 …

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Prospects For Change: Creating A Blended Learning Program Through A Culture Of Support, Bill Leach Jan 2010

Prospects For Change: Creating A Blended Learning Program Through A Culture Of Support, Bill Leach

Electronic Theses and Dissertations

Blended learning, a combination of traditional face to face (F2f) instruction and computer-mediated communication (CMC), is a popular trend in many universities and corporate settings today. Most universities provide faculty members course management systems, such as Blackboard, Angel, and others as a way to organize and transmit course materials to students. In order to assess the pedagogical value of blended learning in a university-level first year composition (FYC) environment, it is necessary to view the environment through a critical lens and adequately train faculty in the need for and use of the features of the learning management software (LMS). The …

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Multi-Modal Reading For Low Level Readers, Jamie O'Neal Jan 2010

Multi-Modal Reading For Low Level Readers, Jamie O'Neal

Electronic Theses and Dissertations

The value of this research hinges on the idea that exchanging illustrations for descriptive text can provide appropriate schemas for students with reading difficulties and thereby improve their comprehension and vocabulary acquisition. The research in this dissertation is based on theories and earlier research in the fields of psychology, education, reading, and narratology. A review of these fields offers a variety of perspectives on the processes involved in reading and comprehension. These processes range from the physical systems involved in reading (e.g., early childhood development, eye movement) to the psychological systems, which include cognitive load theory as well as image …

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A Screen Of One's Own The Tpec And Feminist Technological Textuality In The 21st Century, Amy J. Barnickel Jan 2010

A Screen Of One's Own The Tpec And Feminist Technological Textuality In The 21st Century, Amy J. Barnickel

Electronic Theses and Dissertations

In this dissertation, I analyze the 20th century text, A Room of One's Own, by Virginia Woolf (2005), and I engage with Woolf's concept of a woman's need for a room of her own in which she can be free to think for herself, study, write, or pursue other interests away from the oppression of patriarchal societal expectations and demands. Through library-based research, I identify four screens in Woolf's work through which she viewed and critiqued culture, and I use these screens to reconceptualize "a room of one's own" in 21st Century terms. I determine that the new "room" is …

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Synthesis And Applications Of Ring Opening Metathesis Polymerization Based Functional Block Copolymers, Sanchita Biswas Jan 2010

Synthesis And Applications Of Ring Opening Metathesis Polymerization Based Functional Block Copolymers, Sanchita Biswas

Electronic Theses and Dissertations

Ring opening metathesis polymerization (ROMP) is established as one of the efficient controlled living polymerization methods which have various applications in polymer science and technology fields. The research presented in this dissertation addresses several applications of multifunctional well-defined norbornene-based block copolymers synthesized by ROMP using ruthenium-based Grubbs catalysts. These novel block copolymers were applied to stabilize maghemite nanoparticles, creating the superparamagnetic polymeric nanocomposites. The Jaggregation properties of the porphyrin dyes were improved via self-assembly with a customized norbornene polymer. Novel multimodal copolymer probes were synthesized for two-photon fluorescence integrin-targeted bioimaging. In Chapter 1 a brief overview of ROMP along with …

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The Influence Of High School Dramatic Arts Classes On Remedial Readers On The Florida Comprehensive Assessment Test, Michelle Backel Jan 2010

The Influence Of High School Dramatic Arts Classes On Remedial Readers On The Florida Comprehensive Assessment Test, Michelle Backel

Electronic Theses and Dissertations

This is the age of accountability in public schools. The public wants to know that the schools are producing high achieving students who are ready for the future. With this push for accountability the rise in standardized testing should not be surprising. However, it is difficult to test an abstract course such as the arts. With the increase of standardized testing and the recent tough economic times, it is no wonder that performing arts classes in our public schools are often the first to be pared down or dissolved (Mendels, 2008). It is the presiding feeling that these courses, while …

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The Efficacy Of Effort: Differences In Teachers' Sense Of Efficacy Based On Type Of Teacher Training And Number Of Years Of Experience, Julie Shane Jan 2010

The Efficacy Of Effort: Differences In Teachers' Sense Of Efficacy Based On Type Of Teacher Training And Number Of Years Of Experience, Julie Shane

Electronic Theses and Dissertations

Current federal reforms require a highly-qualified teacher in every classroom to promote higher levels of student performance. In an attempt to provide a sufficient and sustainable number of highly qualified teachers in the workforce, alternative certification training programs have come alongside traditional college of education training programs. Proponents of alternative certification programs contend the process of on-the-job training will potentially address the problem of teacher shortages. However, opponents see these programs as an inadequate training process with future ramification for both teachers and students. As more and more classroom teachers are choosing alternative certification routes, there is growing uncertainty as …

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Addressing Anger Managment In A Middle School Setting: Initiating A Leadership Drive Anger Management Group, Isaac Burt Jan 2010

Addressing Anger Managment In A Middle School Setting: Initiating A Leadership Drive Anger Management Group, Isaac Burt

Electronic Theses and Dissertations

This study examined the effect of a leadership driven anger management group on angry middle school students. Twenty-five participants from a local middle school were nominated by school faculty to be participants in the study. Twenty individuals participated and eighteen completed the study. The eighteen participants were assessed at pre and post test on measures of anger, anger control and personal relationships. Three research questions were tested: (1) Does a short term leadership focused anger management group reduce youths' overall anger? (2) Does such a group increase angry youths' ability to control anger urges? (3) Does this group reduce youths' …

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Surface Plasmon Hosts For Infrared Waveguides And Biosensors, And Plasmons In Gold-Black Nano-Structured Films, Justin Cleary Jan 2010

Surface Plasmon Hosts For Infrared Waveguides And Biosensors, And Plasmons In Gold-Black Nano-Structured Films, Justin Cleary

Electronic Theses and Dissertations

Applications of surface plasmon polaritons (SPPs) have thus far emphasized visible and near-infrared wavelengths. Extension into the long-wave infrared (LWIR) has numerous potential advantages for biosensors and waveguides, which are explored in this work. A surface plasmon resonance (SPR) biosensor that operates deep into the infrared (3-11 µm wavelengths) is potentially capable of biomolecule recognition based on both selective binding and characteristic vibrational modes. The goal is to operate such sensors at wavelengths where biological analytes are strongly differentiated by their IR absorption spectra and where the refractive index is increased by dispersion, which will provide enhanced selectivity and sensitivity. …

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Increasing Parental Awareness And Monitoring: The Development And Evaluation Of A Web-Based Program To Empower Parents To Reduce, Pamela Brown Jan 2010

Increasing Parental Awareness And Monitoring: The Development And Evaluation Of A Web-Based Program To Empower Parents To Reduce, Pamela Brown

Electronic Theses and Dissertations

Parent attitudes about underage alcohol use and parent monitoring of the activities of their adolescent children have been found to be directly related to the likelihood of underage alcohol use. Unfortunately, there are relatively few programs or resources available to parents to assist them to reduce their children's potential for early and problematic alcohol involvement. In an effort to address this need, the present project entailed the development and evaluation of a web-based psychoeducational program entitled, Increasing Parental Awareness and Monitoring (iPAM). This online program begins to fill the gap in effective and convenient programming focused on development of parent …

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Near-Field Optical Interactions And Applications, David Haefner Jan 2010

Near-Field Optical Interactions And Applications, David Haefner

Electronic Theses and Dissertations

The propagation symmetry of electromagnetic fields is affected by encounters with material systems. The effects of such interactions, for example, modifications of intensity, phase, polarization, angular spectrum, frequency, etc. can be used to obtain information about the material system. However, the propagation of electromagnetic waves imposes a fundamental limit to the length scales over which the material properties can be observed. In the realm of near-field optics, this limitation is overcome only through a secondary interaction that couples the high-spatial-frequency (but non-propagating) field components to propagating waves that can be detected. The available information depends intrinsically on this secondary interaction, …

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Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-Cells: Implications For Immunotherapy, Christina Kittipatarin Jan 2010

Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-Cells: Implications For Immunotherapy, Christina Kittipatarin

Electronic Theses and Dissertations

Interleukin-7 (IL-7) is an essential lymphocyte growth factor required for the survival and proliferation of mature T-cells. As a therapeutic agent, IL-7 has the potential to restore T-cell numbers following immune depletion and to promote immunity against cancers. While the survival function of IL-7 is well established, less is known about how it supports T-cell expansion, a critical feature of the immune response. To study the biological effects of IL-7 on T-cell growth, we developed an in vitro culture technique to expand T-cells ex vivo. A significant finding from our studies is that IL-7 did not induce the expansion of …

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Low Cost Production Of Proinsulin In Tobacco And Lettuce Chloroplasts For Injectable Or Oral Delivery Of Functional Insulin And, Diane Burberry Jan 2010

Low Cost Production Of Proinsulin In Tobacco And Lettuce Chloroplasts For Injectable Or Oral Delivery Of Functional Insulin And, Diane Burberry

Electronic Theses and Dissertations

Current treatment for type I diabetes includes delivery of insulin via injection or pump, which is highly invasive and expensive. The production of chloroplast-derived proinsulin should reduce cost and facilitate oral delivery. Therefore, tobacco and lettuce chloroplasts were transformed with the cholera toxin B subunit fused with human proinsulin (A, B, and C peptides) containing three furin cleavage sites (CTB-PFx3). Transplastomic lines were confirmed for site-specific integration of transgene and homoplasmy. Old tobacco leaves accumulated proinsulin up to 47% of total leaf protein (TLP). Old lettuce leaves accumulated proinsulin up to 53% TLP. Accumulation was so stable that up to …

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