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Development Of A Non-Human Primate Model For Staphylococcus Aureus Nasal Carriage, Amanda Lasseter

Electronic Theses and Dissertations

Staphylococcus aureus nasal carriage (SANC) is largely asymptomatic, but presents a risk of autoinfection and dissemination to new immunocompromised hosts. SA disease states range from mild cutaneous infections to life-threatening bacteremia. Historically utilized rodent models do not naturally carry SA in the nose, are insufficient in longitudinal SANC experimentation, and lack immune factors that are vital in human clearance of SA. The nasal passages of non-human primates are similar anatomically and histologically, and reproductive mucosal studies have shown similar immune responses to pathogens and human-relevant microbial profiles. Seventeen captive pigtailed macaques (Macaca nemestrina) were found to naturally carry SA in …

Psychophysiology Meets Computer Science: Predicting The Magnitude Of Participant Physiological Response With Machine Learning, Avonie Parchment

Electronic Theses and Dissertations

The present inquiry uses methods from psychophysiology and machine learning to reduce overall error in classification models. The field of psychophysiology, though rooted in decades of experimentation, has never reached the same level of precision as some aspects of medical inquiry. In fact, while some medical regression models, when determining some way to classify a patient's illness based on certain symptoms, can result in highly significant results with large effect sizes, equal levels are virtually unheard of in psychophysiology. The present investigation attempts to unravel some part of this mystery and determines some possible reasons for the difficulty in finding …

Touching The Unreal: The Definition, Narrative Strategies, And Aesthetics Of 3d Cartoon Narratives., Nathan Snow

Electronic Theses and Dissertations

"Touching the Unreal" follows the structure set out by Scott McCloud in Understanding Comics to argue that understanding cartoons is serious business and requires that we define the art form, outline its basic tenets, and theorize how the mind understands it. The dissertation argues for a new definition of 3D computer generated cartoons, beginning with the most basic definition applicable to all forms of animation and taking into account new technological developments before arriving at the 3D cartoon narratives of today. The dissertation outlines the basic facets of 3D cartoon narratives in terms of narrative and aesthetics, arguing that, in …

Regulation Of Extra-Pituitary Prolactin In Monocytes And Macrophages, Richard Barrett

Electronic Theses and Dissertations

Recently it has been shown that leukocytes are capable of producing prolactin (PRL). Evidence of extra-pituitary PRL (ePRL) production is so far been limited to primates and is not shared across other mammal species such as mice and rats. While ePRL is characterized as an identical protein to traditional pituitary PRL, it is controlled under an alternative promoter and is thus regulated differently from pituitary PRL. Little is known about what regulates ePRL or its direct role in human physiology, but given that PRL has well over 300 described functions, it is likely that the autocrine and paracrine effects of …

Characterization Of Neural Cells Derived From Reelin-Deficient Schizophrenic Patient Ips Cells, Nicole Roberts

Electronic Theses and Dissertations

Reelin is a large, extracellular glycoprotein that binds to several membrane receptors on neural stem cells (HNSCs), neural progenitor cells (NPCs), and neuroblasts of mammals to direct their migration. Previously, our lab established the presence of Reelin increased migration of wild-type fetal-derived HNSC's, both in vitro and in vivo. In addition, we demonstrated that Reelin protein treatment also increases the formation of radial glia via Notch-1 signaling, in vitro. Radial glia are precursors to NPCs, as well as a scaffold for neuroblast migration during cortical lamination. Reelin has long been associated with Schizophrenia (SZ). Because post-mortem brains are limited to …

Retention Of Cardiopulmonary Resuscitation Knowledge And Psychomotor Skill Among Undergraduate Nursing Students: An Integrative Review Of Literature, Fernanda Tirado

Honors Undergraduate Theses

Purpose: The purpose of this integrative literature review is to explore the effectiveness of different training modalities on the acquisition and retention of CPR knowledge and psychomotor skill among undergraduate nursing students. Background: It is well known that standard CPR-training is ineffective at preparing nurses for the rigors of a cardiac arrest event. Survival rates for in-hospital cardiac arrests remain low and the proportion of neurobehavioral sequelae among survivors is very high. Methods: A review of relevant literature published between 2006 and 2016 was conducted using the CINAHL and MEDLINE databases. The following key terms were used in the search: …

Slough: Revealing The Animal, Clay Dunklin

Honors Undergraduate Theses

When making my work I constantly reflect on past mythologies, images, and objects. These served people as a way to make sense of and understand the dynamics of the world around them. As we continue to alter and shape the world into one designed for exclusively human benefit, we need new models that reveal the dynamics of our relationship to the world around us. This is what artists have been doing for centuries, and I specifically look to those using animals and animal imagery in their work to further mythologize our contemporary understanding of the human-other animal relationship. My body …

A Neuroergonomics Study Of Brain Eeg's Activity During Manual Lifting Tasks, Awad Aljuaid

Electronic Theses and Dissertations

Electroencephalography (EEG) has been shown to be a reliable tool in neuroergonomics studies due to the relatively low cost of brain data collection and limited body invasion. The application of EEG frequency bands (including theta, alpha and beta), enjoyed a wide range of interest in physical and cognitive ergonomics. The psychophysical approach has been used for decades to improve safe work practices by understanding human limitations in manual materials handling. The main objective of this research project was to study the brain's EEG activity expressed by the power spectral density during manual lifting tasks related to: 1) the maximum acceptable …

Patterns In Dental Health: Investigating Dental Disease At The Archaeological Site Of Kuelap In Chachapoyas, Peru, Vu Tran

Electronic Theses and Dissertations

Social organization influences individual well-being and overall community health, which may lead to health disparities that manifest in teeth. The research in this thesis explores social variability by analyzing patterns in dental disease at the archaeological site of Kuelap in Chachapoyas, Peru. The ancient Chachapoya (AD 900-1535) were a complex society but the nature of that complexity is not well understood based on traditional archaeological correlates. Since burials at Kuelap lack grave goods that are often used to discriminate variation in social status or identity, bioarchaeological dental proxies were tested. The dental remains of 106 individuals (7 subadults, 54 adult …

Role Of Adrenergic Neurons In Motor Control: Examination Of Cerebellar Purkinje Neurons In Mice Following Selective Adrenergic Cell Ablation In Vivo, Monica Mansour

Honors Undergraduate Theses

Phenylethanolamine-N-methyltransferase (Pnmt) is the enzyme that catalyzes the conversion of noradrenaline to adrenaline. These catecholamines are synthesized in the medulla of the adrenal gland and by some neurons of the central nervous system. The precise location of Pnmt action in the brain and its physiological significance are unknown. Prior studies led by Aaron Owji, a graduate student in Dr. Ebert’s laboratory, showed that mice with selectively ablated Pnmt cells show signs of neurological defects such as abnormal gait, weakened grip strength, lack of balance, reduced movement, and defective reflexes during tail suspension tests.

The cerebellum is a small section of …

Developing Methods For The Estimation Of Stature And Their Use As A Proxy For Health Among The Ancient Chachapoya Of Peru, Armando Anzellini

Electronic Theses and Dissertations

Population mean stature and patterns of health are often linked in anthropological studies, yet few studies control for the multifactorial nature of achieving adult standing height. This thesis explores the intersection of health and stature by analyzing the skeletal remains of 161 adult individuals from the archaeological site of Kuelap, in the eastern slopes of the northern Peruvian Andes, and also tests current biometric methods for estimating stature from skeletal remains. This Chachapoya site dates to the Late Intermediate Period (AD 900 – 1470) and Late Horizon (AD 1470 – 1536) and resides in the high altitude sub-tropical forests of …

Cognitive Flexibility: Using Mental Simulation To Improve Script Adaptation, Javier Rivera

Electronic Theses and Dissertations

Human behavior and decision-making depend largely on past experiences that generate specific action patterns (i.e., scripts, Gioia & Manz, 1985) for specific situations. In an ideal world, in which changes in the environment do not conflict with these action patterns, humans would be able to operate consistently, efficiently, and automatically. However, real-world environments are dynamic and fluid, thus altering behavior and forcing changes in scripts. Research suggests that to implement alternate solutions to changing situations, humans select from a "library" of learned scripts. Since humans tend to implement scripts to the degree that these are successful over a period of …

The Effect Of Color On Working Memory Performance, Michelle Galvez

HIM 1990-2015

This paper explores the effect of color on working memory performance. Interest in Baker-Miller pink surged with the finding by Schauss (1979) that it reduced aggression in aggravated detainees. However, research behind Baker-Miller pink has been influenced by biases and methodological errors. Its effects are likely overstated. Red and blue have also been studied for their effects on creativity, approach-avoidance conflict, detail-orientation, and most importantly, stress. Further research has been conducted on the effects of relaxation on cognition, with the conclusion that increased relaxation leads to improvements in working memory performance. This paper tests the effect of color on working …

Understanding And Modeling Pathways To Thrombosis, John Seligson

HIM 1990-2015

This research will investigate techniques to create a sensor that is responsive to methane at 23°C. The approach will use the integration of a very thin film, which changes its resistive properties when methane gas is applied, deposited atop the surface of a piezoelectric substrate. An aluminum thin film interdigital transducer will launch a surface acoustic wave (SAW) that travels under the sensor’s gas-sensitive resistive thin film. The SAW/resistive film interaction changes the SAW amplitude, phase and delay. For this work, three films, tin dioxide (SnO2), zinc oxide (ZnO) and palladium (Pd) [1, 2] will be studied. Gas detection will …

Differential Expression Of Proteins Involved In Vldl Trafficking Causes Reduced Vldl Secretion In Male Ames Dwarf Mice, Faisal Ahmed Moinuddin

Electronic Theses and Dissertations

Cardiovascular diseases (CVDs) have been recorded as the number one cause of death worldwide, accounting for 32% of total deaths annually. More than two-thirds of all CVD cases are associated with atherosclerosis, which is the accumulation of fats and other substances causing plaque formation in the interior walls of major arteries. This leads to narrowing of the lumen and hardening of the arteries, ultimately resulting in angina, heart attack and/or stroke. Studies have shown that the pathogenesis of atherosclerosis and associated CVDs is strongly linked to elevated secretion of liver-specific lipoproteins called very-low-density-lipoprotein (VLDL). VLDLs are crucial lipoproteins responsible for …

An Investigation Of Physiological Measures In A Marketing Decision Task, Nelson Lerma

Electronic Theses and Dissertations

The objective of the present study was to understand the use of physiological measures as an alternative to traditional market research tools, such as self-reporting measures and focus groups. For centuries, corporations and researchers have relied almost exclusively on traditional measures to gain insights into consumer behavior. Oftentimes, traditional methods have failed to accurately predict consumer demand, and this has prompted corporations to explore alternative methods that will accurately forecast future sales. One the most promising alternative methods currently being investigated is the use of physiological measures as an indication of consumer preference. This field, also referred to as neuromarketing, …

Identification Of The Domain(S) In Protein Disulfide Isomerase Required For Binding And Disassembly Of The Cholera Holotoxin, Laura Herndon

HIM 1990-2015

Cholera, caused by the secretion of cholera toxin (CT) by Vibrio cholerae within the intestinal lumen, triggers massive secretory diarrhea which may lead to life-threatening dehydration. CT is an AB5-type protein toxin that is comprised of an enzymatically active A1 chain, an A2 linker, and a cell-binding B pentamer. Once secreted, the CT holotoxin moves from the cell surface to the endoplasmic reticulum (ER) of a host target cell. To cause intoxication, CTA1 must be displaced from CTA2/CTB5 in the ER and is then transferred to the cytosol where it induces a diarrheal response by stimulating the efflux of chloride …

Rapid Response Teams Versus Critical Care Outreach Teams: Unplanned Escalations In Care And Associated Outcomes, Valerie Danesh

Electronic Theses and Dissertations

The incidence of unplanned escalations during hospitalization is undocumented, but estimates may be as high as 1.2 million occurrences per year in the United States. Rapid Response Teams (RRT) were developed for the early recognition and treatment of deteriorating patients to deliver time-sensitive interventions, but evidence related to optimal activation criteria and structure is limited. The purpose of this study is to determine if an Early Warning Score-based Critical Care Outreach (CCO) model is related to the frequency of unplanned intra-hospital escalations in care compared to a RRT system based on staff nurse identification of vital sign derangements and physical …

Critical Velocity Is Associated With Combat Specific Performance Measures In A Special Forces Unit, Mattan Hoffman

Electronic Theses and Dissertations

Over recent years, military research has focused on ways of being able to predict operational success and readiness through the development of simulated operational tasks measuring the physical limits of the soldier. Therefore, to properly prepare the tactical athlete for the demands and rigor of combat, accurate assessment of baseline physical abilities and limitations are necessary. Currently, western armies use a basic physical fitness test, which has been heavily argued to have no bearing on operational readiness, thus they are in the process of transitioning to a more specific combat readiness test. However, specific assessments to predict operational success/readiness are …

The Effect Of Moderate Altitude On High Intensity Running Performance During A Game With Collegiate Female Soccer Players, Jonathan Bohner

Electronic Theses and Dissertations

Competition often requires teams that reside at sea level to compete against opponents whose residence is at a moderate altitude. This may pose a potential competitive disadvantage considering that moderate altitude may cause decrements in VO2max, distance covered, and time to exhaustion in endurance athletes. The purpose of this study was to examine the effect of altitude on game performance measures. Six NCAA Division I female soccer players (20.33 ± 1.21 y; 168 ± 6.45 cm; 62.5 ± 6.03 kg) were retrospectively examined. Comparisons were made between two competitions that were played at sea level (SL) within two weeks of …

Manipulating Aktivated Metabolism Via Mtorc1, Ivan Von Hack Prestinary

HIM 1990-2015

Although poorly understood, normal cells and cancerous cells of the same type exhibit different patterns of nutrient consumption, processing and utility of metabolic substrates. Differences in substrate uptake, preference, and alternately emphasized metabolic pathways offer opportunities for selective targeting of cancer versus stroma. This may be accomplished by using a sequential approach of nutrient deprivation and pharmaceutical perturbation of metabolic pathways to inhibit cellular proliferation. The purpose of this study was to investigate the effects of restricting glucose and glutamine concentrations, in vitro, to levels that resemble a potential human fasting state. The mammalian target of rapamycin (mTOR), a mediator …

A Computed Tomography-Based Model Of The Infant Hip Anatomy For Dynamic Finite Element Analysis Of Hip Dysplasia Biomechanics, Kyle Snethen

HIM 1990-2015

Newborns diagnosed with hip dysplasia face severe consequences when treatments fail. The Pavlik harness presents the most common worldwide treatment for correcting this medical hip abnormality in newborns, but becomes increasingly ineffective as subluxation increases. A dynamic finite element analysis on the hip joint would yield results that could provide insight to physicians as to how the Pavlik harness could be optimized to increase its success rate and develop patient-specific treatment plans. The study completes the first step in such an analysis by generating a three-dimensional model of an infant hip joint directly derived from computed tomography imaging in order …

Simulation-Based Cognitive Workload Modeling And Evaluation Of Adaptive Automation Invoking And Revoking Strategies, Christina Rusnock

Electronic Theses and Dissertations

In human-computer systems, such as supervisory control systems, large volumes of incoming and complex information can degrade overall system performance. Strategically integrating automation to offload tasks from the operator has been shown to increase not only human performance but also operator efficiency and safety. However, increased automation allows for increased task complexity, which can lead to high cognitive workload and degradation of situational awareness. Adaptive automation is one potential solution to resolve these issues, while maintaining the benefits of traditional automation. Adaptive automation occurs dynamically, with the quantity of automated tasks changing in real-time to meet performance or workload goals. …

Immunomodulation And T Helper Th1/Th2 Response Polarization By Ceo2 And Tio2 Nanoparticles, Brian C. Schanen, Soumen Das, Christopher M. Reilly, William L. Warren, William T. Self, Sudipta Seal, Donald R. Drake Iii

Faculty Bibliography 2010s

Immunomodulation by nanoparticles, especially as related to the biochemical properties of these unique materials, has scarcely been explored. In an in vitro model of human immunity, we demonstrate two catalytic nanoparticles, TiO2 (oxidant) and CeO2 (antioxidant), have nearly opposite effects on human dendritic cells and T helper (T-H) cells. For example, whereas TiO2 nanoparticles potentiated DC maturation that led towards T(H)1-biased responses, treatment with antioxidant CeO2 nanoparticles induced APCs to secrete the anti-inflammatory cytokine, IL-10, and induce a T(H)2-dominated T cell profile. In subsequent studies, we demonstrate these results are likely explained by the disparate capacities of the nanoparticles to …

Role Of Host Immune Response And Bacterial Autolysin Atl In Human Nasal Colonization By Staphylococcus Aureus, Vanathy Paramanandam

Electronic Theses and Dissertations

Staphylococcus aureus (SA) is a major human pathogen that colonizes the anterior nares in 30% of the human population. Though nasal carriage of SA is a known risk factor for the subsequent spread of SA infections, the dynamics of SA nasal colonization is poorly understood. Our research focuses on understanding the host and bacterial factors that might contribute to the human nasal colonization by SA. In an attempt to elucidate the host response to SA, we performed an autologous human in vivo nasal colonization study, which showed decreased survival rates of SA in hosts who elicited a robust immune response. …

Investigation Of Multiscale Fluid Structure Interaction Modeling Of Flow In Arterial Systems, Sebastian Sotelo

Electronic Theses and Dissertations

The study of hemodynamic patterns in large blood vessels, such as the ascending aortic artery, brachiocephalic trunk, right carotid artery and right subclavian artery presents the challenging complexity of vessel wall compliance induced by the high levels of shear stress gradients and blood flow pulsatility. Accurate prediction of hemodynamics in such conditions requires a complete Fluid Structure Interaction (FSI) analysis that couples the fluid flow behavior throughout the cardiac cycle with the structural response of the vessel walls. This research focuses on the computational study of a Multiscale Fluid-Structure Interaction on the arterial wall by coupling Finite Volumes Method (FVM) …

Gender Differences And Fast Food Preferences Among U.S. College Students, Donald Persaud

Electronic Theses and Dissertations

The intent of this research is to examine the relationship of corporate fast food and health within the context of gender. It tests the hypothesis that Western perceptions of masculinity and femininity inform a differentiated pattern of food preference, which will correlate with health as measured by Body Mass Index (BMI). The targeted sample population for the study is the undergraduate community of the University of Central Florida, and fast food is a chosen dietary medium because it is an increasingly ubiquitous source of caloric energy in the American foodscape, representative of the mass production model applied to cuisine in …

The Effect Of Music On Physiological Responses And Self-Perceived Mood, Robert-Christian Sanchez

HIM 1990-2015

Music is often studied in terms of its artistic value and expressiveness. While these are important characteristics, there are other observations we can make of scientific value, such as the effects of music on the human anatomy. At present, however, there is a general lack of scientific studies focusing on the effect music makes on specific physiological responses in the body. A limited range of these studies has included examinations of music preferences and correlating personality characteristics of participants, while some others have investigated the effects of music lessons on intelligence. While the previously mentioned research has contributed to some …

Adding Cerebral Autoregulation To A Lumped Parameter Model Of Blood Flow, Russell Gentile

HIM 1990-2015

A mathematical model of blood flow in infants with hypoplastic left heart syndrome (HLHS) was improved by adding cerebral autoregulation. This is the process by which blood vessels constrict or dilate to keep blood flow steady in certain organs during pressure changes. The original lumped parameter model transformed the fluid flow into an electrical circuit. Its behavior is described using a system of thirty-three coupled differential equations that are solved numerically using a fourth-order Runge-Kutta method implemented in MATLAB. A literature review that includes a discussion of autoregulation mechanisms and approaches to modeling them is followed by a description of …

Rafts, Nanoparticles And Neural Disease, Vishal Gulati, Ron Wallace

Faculty Bibliography 2010s

This review examines the role of membrane rafts in neural disease as a rationale for drug targeting utilizing lipid-based nanoparticles. The article begins with an overview of methodological issues involving the existence, sizes, and lifetimes of rafts, and then examines raft function in the etiologies of three major neural diseases-epilepsy, Parkinson's disease, and Alzheimer's disease-selected as promising candidates for raft-based therapeutics. Raft-targeting drug delivery systems involving liposomes and solid lipid nanoparticles are then examined in detail.