Digital Commons Network^™

Monitoring Shifts In Estuarine Microbial Communities: A Dna-Metabarcoding Approach, Ashley M. Reaume

Graduate Studies Theses and Dissertations 2026

Estuarine microbial communities, including plankton, algae, and bacteria, play important roles in nutrient cycling and food web structure. They are sensitive to changes in abiotic conditions, and shifts in community composition can provide insight into estuarine ecosystem functioning in the face of various physiochemical and anthropogenic stressors. Therefore, monitoring these communities is essential for understanding and maintaining estuarine health. DNA metabarcoding is an emerging technique for enhancing biological monitoring, allowing for the detection of multiple species within a single sample. This dissertation first focuses on how methodological decisions impact DNA metabarcoding results and then implements these optimized methods to perform …

Detection And Discrimination Of Targets In Infrared Imagery, Adam T. Cuellar

Graduate Studies Theses and Dissertations 2026

Automated infrared (IR) imagery analysis is essential for persistent surveillance, defense, and security, yet it remains difficult when sensors move and when unknown objects appear. This dissertation addresses two fundamental problems: (1) detecting small moving targets amid platform-motion induced parallax and (2) distinguishing between known stationary targets and out-of-distribution (OOD) objects.   For detecting moving targets while the platform itself is in motion, auxiliary Global Positioning System and Inertial Navigation System data are combined with image analysis. Direction Cosine Matrices from calibrated inertial measurements enable sub-pixel frame alignment unattainable with purely image-based registration. The stabilized sequence is processed by a Reed–Xiaoli …

Establishment Of An In Vitro Dss-Induced Epithelial Injury Model To Investigate Il-6 Signaling And Microbial Metabolite Mediated Barrier Recovery, Srijal Kunwar

Graduate Studies Theses and Dissertations 2026

Dextran sodium sulphate (DSS) is widely used in vivo to induce epithelial injury; however, the molecular effects of DSS on intestinal epithelial cells, that investigate inflammatory and metabolic repair mechanisms remain unclear. Here, we establish an in vitro DSS-induced epithelial damage model using differentiated Caco-2 cell monolayer. Exposure to 2.5%(w/v) DSS for 24 hours resulted in significant upregulation of pore forming protein Claudin-2(2.677-fold) and oxidative marker, NOX-1(1.866-fold), accompanied by increased ROS production confirmed via DHE staining. Increased epithelial permeability was observed by TEER analysis, without significant cytotoxicity. Surprisingly, ELISA analysis revealed significant downregulation of IL-6 (p-value …

Careless Responding: Testing The Theory Of Vigilance, Rusty Wilson

Graduate Studies Theses and Dissertations 2026

Careless responding (CR) has been identified as a threat to the psychometric integrity of cognitive and non-cognitive tests, with much of the current research focusing on the identification and removal of carelessness from dataset. While this research has proved fruitful in improving data quality, there has been a recent push to move towards preventing carelessness as opposed to post-hoc removal, which harms statistical power. However, the most common prevention strategy to prevent carelessness (pre-survey warnings) has shown equivocal effects. Additionally, the literature lacks an agreed upon theory to explain why carelessness occurs. To address these gaps I introduce theory from …

Evaluating Novel Targeted Combination Therapies For Gastrointestinal Cancers, Joseph A. Goode

Graduate Studies Theses and Dissertations 2026

Gastrointestinal (GI) cancers remain among the leading causes of cancer-related mortality worldwide, with therapeutic resistance continuing to limit the effectiveness of current treatments. Three complementary studies were conducted to identify therapeutic vulnerabilities and evaluate novel combination strategies in pancreatic ductal adenocarcinoma (PDAC) and colorectal cancer (CRC).  First, dual inhibition of fibroblast growth factor receptor 4 (FGFR4) and phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) signaling was investigated in PDAC models. A combination of targeted drug treatments reduced viability, clonogenic survival, migration, and suppressed signaling pathways involved in translational control. This supported a functional interaction between FGFR4 and PI3K-mTOR co-activation in …

Investigating The Impact Of School Leaders’ Leadership Style And Teachers’ Intrinsic Motivation For Improved Science Instruction In Elementary Schools, Leila M. Greene

Graduate Studies Theses and Dissertations 2026

A report by the National Academies of Sciences, Engineering, and Medicine, determined that 69% of U.S. elementary teachers are not prepared to teach science, especially in the areas of earth/space and physical sciences. As a result, some elementary teachers avoid teaching science. School administrators play a key role in providing a supportive environment to inspire and motivate elementary science teachers. For this reason, the study utilized the Self-Determination Theory (SDT), to examine three types of school leadership styles (Transformational, Transactional, and Laissez-Faire) and how they impact the intrinsic motivation for elementary science teachers in three areas including autonomy, competency, and …

Unmasking Pentastomes: Generating Genomic Resources For Enigmatic Parasites, Stephanie Lyn Villella

Graduate Studies Theses and Dissertations 2026

The introduction of invasive species can disrupt ecosystems not only through competition and predation but also by introducing novel pathogens to naïve host populations. In the southeastern United States, the invasive Burmese python (Python bivittatus) has been implicated in the introduction of the pentastome parasite Raillietiella orientalis, which has been associated with increased parasite burdens and mortality in native snakes. Despite their ecological significance and ancient evolutionary history, pentastomes remain poorly represented in genomic databases, limiting efforts to understand their evolutionary origins, host–parasite interactions, and mechanisms underlying parasite emergence. This study addresses these gaps by generating the …

Site Use And Demographic Changes In A Northwest Atlantic Sea Turtle Foraging Ground, Ryan C. Welsh

Graduate Studies Theses and Dissertations 2026

and site fidelity, making mortality difficult to distinguish from redistribution. We developed a behaviorally explicit framework to separate true mortality from changes in availability caused by movement and redistribution. We evaluated this problem for juvenile green turtles (Chelonia mydas) and loggerhead turtles (Caretta caretta) captured at the St. Lucie Nuclear Power Plant intake canal on Florida’s Atlantic coast using a multi-decade capture–mark–recapture record spanning 1977 - 2023. Hidden Markov Models classified individuals into latent habitat-use strategies (Nomadic, Resident, and Transient) based on capture timing and body size. Across both species, these strategies captured consistent differences in site fidelity and recapture …

Ecological And Evolutionary Responses To Environmental Change In Two Large North American Mammals, George Zaragoza

Graduate Studies Theses and Dissertations 2026

Climate change and urbanization are resulting in rapid shifts in ecosystems. The effects of these changes are complex, and understanding how species respond is critical for predicting their impacts on genetic diversity and evolution, as well as changes in habitat suitability. Species can respond to these changes in two major ways: through behavioral or genetic adaptation, or by moving to more suitable habitat as conditions change. Adaptive genetic change, in particular, can support behavioral and physiological responses that enable species to persist in novel or changing environments, and understanding these changes can help predict how species will respond to anthropogenic …

The Uncanny And Uneasy: An Exploration Into Using Puppets To Share Dark Stories, Grace Auls

Graduate Studies Theses and Dissertations 2026

In this thesis, I will explore the role of puppetry and practical effects in evoking the uncanny and the uneasy as well as questioning the implications they have in dark theater and cinematic contexts. This paper will examine how puppetry and practical effects can contribute to potentially disturbing and dark imagery both on screen and in live theater. I will be focusing on looking at Jim Henson’s The Dark Crystal, as well as at different puppetry performances used in Voyage of the Little Mermaid (1992), Don’t Hug Me I’m Scared, Kirikou and the Sorceress (Kirikou et la Sorciere), and even …

Reclaiming The Self: A Visual Narrative Of A Process Of Individuation Through Artistic Practice, Stacie Becker

Graduate Studies Theses and Dissertations 2026

Reclaiming the Self: A Visual Narrative of a Process of Individuation Through Artistic Practice features a multidisciplinary practice that uses visual narrative, metaphor, and symbolism to reconcile the complex intersections of internal and external identity-shaping experiences, informed by lived experience as a woman. Working across painting, mixed media, printmaking, book arts, sculpture, and restorative medical tattooing, the artist dissects the tension between internal identity and external expectations, addressing themes of gender roles, relationships, trauma, and agency. Various artistic methods serve not only as a process but also as a parallel for reclaiming, healing, and restoring an authentic, unashamed sense of …

The Place Between Places, Harrison Burke

Graduate Studies Theses and Dissertations 2026

The Place Between Places is a collection of essays centered on belonging. Focusing on the composition of city streets, theme parks, and hidden pockets of wonder, belonging is explored as the product of place and culture. The perspective of the collection shifts between reflection and active exploration, using present moment journeys as catalysts to unravel the past. In every new discovery there is a ghost, past and present journeys fighting for their place in the constructed narrative called “self.”

The collection primarily makes use of braided essays and nonlinear storytelling. “Animal” blends together three night walks through New York, Seoul, …

Taming Wildfires, Bianca Dagostino

Graduate Studies Theses and Dissertations 2026

Taming Wildfires is a novella immersed in the mind of a young woman named Lilla with intense, undiagnosed mental illness attempting to reenter society after losing the crutch of her long-term partner. The novella engages with the intersection of queerness, mental illness, interracial relationships, and the society of recent past (2023-2024), and deals with themes of isolation, loss, self-image, and emotional healing. Readers are invited fully into Lilla’s mind as she navigates a long-term friendship strained by proximity and a new romance marred by omission. These relationships are overcast by her loss, her skewed reality, and her conflicting desires to …

Improvisational Theatre And Theatre Of The Oppressed: Fostering Growth In Myself And Beyond, Leigh S. Green

Graduate Studies Theses and Dissertations 2026

This thesis examines how improvisational theatre and Augusto Boal’s Theatre of the Oppressed function independently and how these practices have shaped me as both an artist and educator. Having grown up with improvisation and continued working professionally in performance and education, I have observed how it creates conditions that support development. Guided by a qualitative practice-as-research methodology, in which my own artistic and pedagogical practice serves as both site and subject of inquiry, this study explores three central questions: How does improvisational theatre contribute to youth development? In what ways can Theatre of the Oppressed deepen or reshape improvisation’s developmental …

Centered A Story Of Family And Compassion In The Face Of Parkinson's Disease, Savannah Hill

Graduate Studies Theses and Dissertations 2026

Centered is a three-dimensional (3D) animated short film that explores the topic of parental illness and how families must respond with compassion and adaptability when loved ones face mental or physical challenges. The story is inspired by the director’s own experience with her father’s diagnosis of Parkinson’s Disease when she was in high school. The main characters, a father and daughter, must navigate the father’s changing needs after his diagnosis by prioritizing their connection and adapting to his new physical challenges. In the film, they do this through the creation of pottery. The imperfect, handmade design style of the film …

Specters, Fred Lambert

Graduate Studies Theses and Dissertations 2026

Specters is a collection of interconnected short fiction that explores the lives of various characters affected by the Iraq War. Touching upon themes of mortality, dehumanization, guilt, and the trauma inflicted by senseless violence, the stories in this collection aim to blur the line between the concrete and the phantasmagoric. The titular story follows a young Marine whose grip on reality is called into question during the Second Battle of Fallujah. In “Demons,” a recruit undergoing the chaotic and brutal grind of Parris Island begins to wonder if his drill instructors are possessed. “Kill Bodies” chronicles the repeated killing of …

Cell Type-Specific Fatty Acid Signalling And Its Sex Dependence, Emeline Masterson

Graduate Studies Theses and Dissertations 2026

Dietary fat is a highly palatable, energy-dense nutrient whose oral detection powerfully shapes ingestion patterns and metabolic outcomes. Despite clear behavioral and physiological evidence that fat can evoke distinct taste signals, the cellular and molecular mechanisms underlying its transduction remain poorly understood. A critical gap exists in our understanding of the cell-type-specific molecular pathways and how these signals are modulated by hormonal status. This dissertation delineates estrogen-dependent plasticity in FA transduction within the peripheral taste system, revealing coordinated, sex and cell-type-specific modulation of these pathways. To understand the missing mechanisms, FA-evoked responses were compared in Type II cells from wild-type …

Exploring The Role Of Hes4 During Retroviral Infection, Raphael Opoku

Graduate Studies Theses and Dissertations 2026

Human immunodeficiency virus (HIV) infection disrupts immune homeostasis and impairs antiviral defenses, partly by compromising natural killer (NK) cell activity, with simian immunodeficiency virus (SIV) infection in rhesus macaques serving as a valuable model to investigate these mechanisms. This research examines the regulatory role of HES4 and its isoforms in NK cell function during retroviral infection, with particular focus on how Notch signaling–dependent regulation of HES4 shapes NK effector responses. HES4, a downstream effector of the Notch signaling pathway, encodes transcriptional repressors central to immune cell differentiation, yet its contribution to NK cell biology remains poorly understood. Using molecular and …

Magic In The Margins: A Painter's Ode To Love, Loss, And Laughter, Angelina B. Palmisano

Graduate Studies Theses and Dissertations 2026

In art, there lives a gift of transcendence with power to transport the viewer into another realm of thought and imagination beyond the limitations of our physical world. This thesis, Magic in the Margins: A Painter’s Ode to Love, Loss, and Laughter, is an exploration of the ways art can serve as both sanctuary and vessel for storytelling. Satire is used to criticize vices, and subtle humor is my tool to resonate serious content necessary for authentic self-reflection. I am investigating how to use painting as a means to make deeply intimate and visceral connections. Each canvas exploring the innermost, …

Source Credibility And Chatbots: How Design Factors Impact Chatbot Effectiveness, Kristen M. Schmidt

Graduate Studies Theses and Dissertations 2026

Source credibility theory states that the more credible a source appears to be, the more likely one is to believe both true and untrue information from it. In the modern day, misinformation is considered a major problem, particularly with virtual communication, meaning it is essential to study what factors influence a system’s source credibility. Virtual agents are a particularly relevant subject of study given that their source credibility can be impacted by both technological and social factors. Across three experiments, this dissertation utilized a novel chatbot interaction design to test how varying design factors would impact a chatbot’s source credibility …

Forest Island Reforestation In The Llanos De Moxos, Bolivia, Ethan Tam

Graduate Studies Theses and Dissertations 2026

Within the Llanos de Moxos of Bolivia thousands of forest islands dot the landscape. These forest islands have been shown to have been integral aspects of the pre-Contact indigenous cultures which existed there for thousands of years. Based on 1967 CORONA imagery and statistical analysis, this work provides insight into how these forest islands are integrated as infrastructure features within their landscape and surrounding network of other archaeological features. This research is carried out through investigations into changes in the size of the forest islands across an interval of 57 years, statistical attempts to categorize the forest island into smaller …

Building Eden Where We Can, Ireland Thomas

Graduate Studies Theses and Dissertations 2026

Building Eden Where We Can is a nonfiction memoir told in vignettes that come together to build a wider story. Through these vignettes, we are shown a narrator who is simultaneously surviving an abusive family, navigating a rare diagnosis, and coming to terms with their queerness. By braiding narratives and maintaining these various threads, this collection examines the intersections of sickness and religion, tradition and freedom, as well as fear and love. The lattermost dichotomy is represented in the narrator’s ultimate choice to leave behind a life of fear in the mother figure’s safe cage. In exchange, they receive a …

The Effects Of Episodic Facilitation And Immersion On Memory Formation During Learning Of An Exterior Preflight Inspection Procedure, Nathan A. Sonnenfeld

Graduate Studies Theses and Dissertations 2026

This research examined the use of episodic facilitation—instructional elements aligned with episodic memory (EM) processes—to affect learners’ memory of simulation training for an aviation procedure. Despite its assumed benefits in industry and theory, virtual reality (VR) has shown limited advantages over conventional media for training procedures, in part because prevailing frameworks emphasize semantic and procedural outcomes, leaving memory of training experiences as events underexamined. To address this gap, this work advances human factors research by examining EM as a design consideration, an outcome, and an explanatory mechanism for how simulation training experiences are remembered. Grounded in theories of EM and …

Using Inferences From Natural Language Narratives To Improve Video Question Answering, Hoang Bao Truong

Graduate Studies Theses and Dissertations 2026

The purpose of this thesis is to examine how structured narrative representation and reasoning strategies jointly affect the performance of Video Question Answering (VideoQA). Although recent advances in large multimodal and language models have improved video description and retrieval, causal and counterfactual reasoning remain challenging. This thesis studies an inference-only pipeline in which videos are converted into natural-language narratives, and those narratives may optionally be transformed into a structured NarrativeML format before multiple-choice questions are answered without task-specific fine-tuning. NarrativeML is a machine-readable narrative representation that explicitly encodes entities, events, and their causal and temporal relations. Three representational contexts are …

Sonic Costumes For Immersive Theme Park Storytelling: Developing Interactive Wearables To Enhance Performer, Audience, And Environment Interaction, Rachel Yee

Graduate Studies Theses and Dissertations 2026

Costumes enhance the themed experience through visual storytelling while also allowing performers to interact fluidly with a physical space. These garments are typically passive storytellers in theme parks, even if the performers interact directly with guests. Some garments coordinate with and react to elements of the themed environment, predominantly when worn by performers within live shows, parades, or streetmosphere settings. However, this interactivity is often available only at a distance from guests, causing a separation between audience and performer. For instance, lights on parade costumes, such as those throughout Disney’s Magic Kingdom Park, often synchronize only with parade floats and …

Fgf19 As A Colorectal Cancer Serum Biomarker And Fgfr4 As A Mediator Of Anti-Her2 Resistance In Breast Cancer, Jordan Beardsley

Graduate Studies Theses and Dissertations 2026

The fibroblast growth factor 19 (FGF19)-fibroblast growth factor receptor 4 (FGFR4) signaling pathway regulates hepatic metabolism, bile acid (BA) synthesis, and hepatocyte proliferation. This signaling axis also is implicated in promoting tumor progression, therapeutic resistance, and metastasis in certain cancers.

In colorectal cancer (CRC) there is a critical need for non-invasive biomarkers for disease detection and monitoring. We identified FGF19 as a candidate CRC serum marker through meta-transcriptomics and found FGF19 to be expressed and secreted by CRC cell lines. Tumor-derived FGF19 was detectable in mouse serum at levels correlating with tumor volume in a subcutaneous xenograft model. RNA sequencing …

Pioglitazone Uncouples Macrophage Inflammatory Signaling From Epithelial Oxidative Stress During Map Infection Mimicking Crohn’S Disease Treatment, Sabera Akter Bushra

Graduate Studies Theses and Dissertations 2026

Crohn’s disease (CD) is a chronic inflammatory bowel disease characterized by intestinal inflammation, epithelial barrier dysfunction, and abnormal immune signaling. CD pathophysiology is increasingly linked to Mycobacterium avium subspecies paratuberculosis (MAP) infection, which increases chronic macrophage activation and the production of inflammatory mediators that lead to epithelial damage. Among these mediators, CXCL10, TNF-α, and IL-6 contribute in inflammation, while NOX-1 and SERPINE-1 indicate epithelial oxidative stress and damage. Despite the role of macrophage-epithelial interaction in CD, selective pharmaceutical regulation of this axis remains unclear. Pioglitazone, an FDA-approved PPARγ agonist used for type 2 diabetes, is an appealing repurposing candidate, given …

Integrated Optical Probes For Confocal Scanning Imaging And Adjustable Coherent-Gated Dynamic Sensing, Yonglin Huang

Graduate Studies Theses and Dissertations 2026

Optics and photonics have been one of the most important sciences and technologies that impact modern human life in a big way. For example, fiber-optics for communications and artificial intelligence. Optical probes are critical components for optical imaging and optical sensing technologies that have been actively researched and developed in the past decades. Advanced fiber-optic sensor probes with smaller size, better performance, lower noise, higher photon efficiency, rapid sensing time, and lower cost are needed in many applications, such as nanoscale material science, chemistry, and biomedical fields, etc.  In this project, new fiber-optic sensor probe technologies and integrated micro-optic devices …

Towards An Understanding Of Learning In Immersive Virtual Reality: Investigating Learning Affordances To Bridge Theory And Practice, Gregory Mcgowin

Graduate Studies Theses and Dissertations 2026

This dissertation examines how modality-specific scaffolding shapes performance, presence, and transfer in immersive virtual reality (I-VR). Framed through theories of extended cognition, real-time guidance is conceptualized as cognitive scaffolding that structures perception and action by embedding task-relevant information within the environment. Situated within the learning sciences and informed by 4E cognition (embodied, embedded, enactive, extended), this work develops a framework for understanding how I-VR affordances relate to learning and performance.

The empirical study investigates how scaffolding modality (audio vs. visual) influences presence and performance in a VR racing simulation. Using a 3 × 2 × 2 mixed factorial design, 111 …

“How Far Do These Roots Go Down”: An Exploration Of Latinx And Latina Fans On Representation In Disney's Encanto, Noemi Nunez

Graduate Studies Theses and Dissertations 2026

In this dissertation, I examine how Latinx and Latina audiences engaged with and interpreted Disney’s 2021 Encanto, an animated film celebrated for its representation of Colombian and broader Latinx culture. While scholarship on Latinx representation in media has expanded, less attention has been given to how Latinx audiences themselves respond to narratives that are meant to represent them, particularly within fan studies. Focusing on both a broader Latinx fan community and first-generation Latinas in the United States, this project explores how audiences negotiate representation and recognition using Encanto as a media text that centered a Latinx story. Drawing from …