Digital Commons Network^™

Mitigating Toxic Metal Exposure Through Leafy Greens: A Comprehensive Review Contrasting Cadmium And Lead In Spinach, Angelia L. Seyfferth, Matthew A. Limmer, Benjamin R. Runkle, Rufus L. Chaney

Biological Sciences Faculty Publications and Presentations

Metals and metalloids (hereafter, metal(loid)s) in plant-based foods are a source of exposure to humans, but not all metal(loid)-food interactions are the same. Differences exist between metal(loid)s in terms of their behavior in soils and in how they are taken up by plants and stored in the edible plant tissue/food. Thus, there cannot be one consistent solution to reducing toxic metal(loid)s exposure to humans from foods. In addition, how metal(loid)s are absorbed, distributed, metabolized, and excreted by the human body differs based on both the metal(loid), other elements and nutrients in the food, and the nutritional status of the human. …

A Cost-Effective Enzyme Kinetics And Inhibition Model For Biochemistry Education And Research, Lauren Vandee, Alyssa Teague, Taylor East, Kim Rhona Jacinto, Macie Carter, Jacey Totty, Paul D. Adams, Djamali Muhoza

Chemistry & Biochemistry Faculty Publications and Presentations

Enzyme kinetics and inhibition studies are crucial in biochemistry education and research. Conventional methods often require expensive equipment and reagents, potentially limiting their accessibility in limited resource settings. Our approach sought to develop a cost-effective experimental design for studying enzyme kinetics and inhibition. Lactase was chosen as a protein model and its activity was investigated by measuring glucose production from lactose hydrolysis. In the study, commercially available lactase pills were used as an enzyme source, while milk was used as a substrate. Instead of scientific equipment, glucometers were used to measure lactase activity. Enzyme kinetics were evaluated using Michaelis–Menten and …

Current And Future Environmental Suitability For Bats Hosting Potential Zoonotic Pathogens In Rural Kenya, Ruut Uusitalo, Reilly T. Jackson, Tamika J. Lunn, Essi M. Kohonen, Joseph G. Ogala, Paul W. Wabala, Tarja A. Sironen, Kristian M. Forbes

Biological Sciences Faculty Publications and Presentations

Synanthropic bats live in close proximity to humans and domestic animals, creating opportunities for potential pathogen spillover. We explored environmental correlates of occurrence for a widely distributed synanthropic African bat, Mops pumilus—a species associated with potential zoonotic viruses—and estimated current and future environmental suitability in the Taita Hills region and surrounding plains in Taita–Taveta County in southeast Kenya. To project future environmental suitability, we used four Coupled Model Intercomparison Project Phase 6 general circulation models that capture temperature and precipitation changes for East Africa. The models were parameterized with empirical capture data of M. pumilus collected …

Slime Molds (Myxomycetes) Causing A “Disease” In Crop Plants And Cultivated Mushrooms, Zhaojuan Zhang, Chao Zhi, Yu Li, Steven L. Stephenson, Pu Liu

Biological Sciences Faculty Publications and Presentations

Myxomycetes (plasmodial slime molds) are eukaryotic protist predators that are associated with wood, leaf litter, and soil in forests, where they feed on bacteria, protozoans, and (to a more limited extent) fungi. The health of crop plants is essential because they represent a primary food source for humans. However, when myxomycetes produce numerous fruiting bodies on the stems and leaves of crop plants, which is herein referred to as a myxomycete colonization, this has the potential of interfering with plant photosynthesis, transpiration and respiration by blocking out light and covering stomata. Myxomycetes are not pathogens, but their occurrence on plants …

The Effects Of Occupational Disruption During Covid-19 Lockdowns On Health: A Cross-Sectional Study, Bernard Austin Kigunda Muriithi, R. Curtis Bay

Communication Disorders and Occupational Therapy Faculty Publications and Presentations

The disruption in daily activity performance during COVID-19 lockdowns is widely understood to have impacted health, but a better understanding of how restricted performance of specific activities are associated with health is needed. This cross-sectional study answers the following question: How were changes in the performance of 16 daily activities associated with health during COVID-19 lockdowns? A total of 116 participants completed an online survey rating their health before and during COVID-19 lockdowns and comparing their recollection of the performance of 16 activities before COVID-19 with their performance during lockdowns. Multiple stepwise linear regression analysis was used to estimate the …

A Multi-Tiered Mentoring Community Approach To Research Experiences For Local Students From Disadvantaged And Underrepresented Minority Backgrounds, Thomas Mckean, Lashall Bates, Gary Bates, Jacquelyn D. Wiersma-Mosley, S. Ranil Wickramasinghe

Chemical Engineering Faculty Publications and Presentations

Introduction: A research and mentoring program was developed to provide local first-generation students, students returning to school after a professional experience, and underrepresented minority students resources and relationships to guide them toward a STEM degree from a four-year university.

Methods: A multi-tiered mentoring community was formed including direct mentoring from graduate students and faculty advisors, peer mentoring among undergraduate students from different colleges and universities, and high school students to increase the accessibility of research opportunities for this demographic. Local students were recruited from Northwest Arkansas Community College and Upward Bound to combine community college and high school students in …

Identifying Dietary Timing Of Organic Trace Minerals To Reduce The Incidence Of Osteomyelitis Lameness In Broiler Chickens Using The Aerosol Transmission Model, Khawla Alharbi, Andi Asnayanti, Anh Dang Trieu Do, Ruvindu Perera, Layla Al-Mitib, Abdulkarim Shwani, Marco A. Rebollo, Michael T. Kidd, Adnan Ali Khalaf Alrubaye

Poultry Science Faculty Publications and Presentations

Our prior research demonstrated a 20% to 25% reduction in bacterial chondronecrosis with osteomyelitis (BCO) lameness in broilers with organic Zn, Mn, and Cu (Availa^® ZMC) supplementation. Expanding on this, we investigated the optimal timing for Availa^® ZMC feeding to mitigate BCO lameness and reduce feed additive costs in the poultry industry. In this study, we compared the application of 0.15% Availa^® ZMC for 56 days, the first 28 days, and the last 28 days. The experimental design was a randomized block design involving 1560 one-day-old chicks distributed across two wire-floor pens as BCO source infection and …

The Effects Of Nano-Curcumin On Growth Performance, Feed Utilization, Blood Biochemistry, Disease Resistance, And Gene Expression In European Seabass (Dicentrarchus Labrax) Fingerlings, Samyah Justaniah, Ahd. A. Mansour, Ayat H. Al-Tarawni, Ahab El-Haroun, Mohhamed Bodrul Munir, Sultan Ayesh M. Saghir, Zulhisyam Abdul Kari, G. Tellez-Isaias, Walter G. Bottje, Ammar Mohammed Hamood Al-Farga, El-Sayed Hemdan Eissa

Poultry Science Faculty Publications and Presentations

Phytochemicals are used in fish farming to reduce stress and combat diseases during intensification. Recently, nanotechnology has represented a paradigm shift in the aquatic feed industry to improve the solubility, availability, and efficacy of phytochemicals. The purpose of this research was to investigate the effects of dietary supplementation with nano-curcumin (CUNE) on the growth, feed utilization, body composition, blood biochemistry, antioxidant status, disease resistance to Vibrio parahaemolyticus (V. parahaemolyticus), and the expression of insulin-like growth factor 1 (IGF-1), growth hormone (GH), interleukin-10 (IL-10), and interlukin-1β (IL-1β) genes in European Seabass (Dicentrarchus …

Microbial Symbiont-Based Detoxification Of Different Phytotoxins And Synthetic Toxic Chemicals In Insect Pests And Pollinators, Olivia Kline, Neelendra K. Joshi

Entomology and Plant Pathology Faculty Publications and Presentations

Insects are the most diverse form of life, and as such, they interact closely with humans, impacting our health, economy, and agriculture. Beneficial insect species contribute to pollination, biological control of pests, decomposition, and nutrient cycling. Pest species can cause damage to agricultural crops and vector diseases to humans and livestock. Insects are often exposed to toxic xenobiotics in the environment, both naturally occurring toxins like plant secondary metabolites and synthetic chemicals like herbicides, fungicides, and insecticides. Because of this, insects have evolved several mechanisms of resistance to toxic xenobiotics, including sequestration, behavioral avoidance, and enzymatic degradation, and in many …

Occurrence Of Lotmaria Passim In Africanized And European Honey Bee, Apis Mellifera, Lineages From The United States, Mary-Kate Williams, Dylan Cleary, Allen Szalanski

Entomology and Plant Pathology Faculty Publications and Presentations

Honey bee populations in the United States have been under stress for the past several decades. Several internal parasites may contribute to this, including the trypanosome Lotmaria passim. It is unknown how widespread the honey bee parasite, L. passim, is in Africanized honey bee (AHB) populations in the United States. A total of 321 feral honey bee colony samples which had been previously recorded to be of Africanized origin using molecular diagnostics from California (n=3), New Mexico (n=46), Oklahoma (n=57), Texas (n=106), and Utah (n=109) were used in this study. Of these samples, a total of 15 (4.7%) …

Recombinant Bile Salt Hydrolase Enhances The Inhibition Efficiency Of Taurodeoxycholic Acid Against Clostridium Perfringens Virulence, Tahrir Alenezi, Bilal Alrubaye, Ying Fu, Janashrit Shrestha, Samar Algehani, Hong Wang, Rohana Liyanage, Xiaolun Sun

Chemistry & Biochemistry Faculty Publications and Presentations

Clostridium perfringens is the main pathogen of chicken necrotic enteritis (NE) causing huge economic losses in the poultry industry. Although dietary secondary bile acid deoxycholic acid (DCA) reduced chicken NE, the accumulation of conjugated tauro-DCA (TDCA) raised concerns regarding DCA efficacy. In this study, we aimed to deconjugate TDCA by bile salt hydrolase (BSH) to increase DCA efficacy against the NE pathogen C. perfringens. Assays were conducted to evaluate the inhibition of C. perfringens growth, hydrogen sulfide (H₂S) production, and virulence gene expression by TDCA and DCA. BSH activity and sequence alignment were conducted to select the …

Summaries Of Arkansas Cotton Research 2023, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

With current production costs and cotton prices, either record or near-record yields are needed for profitable cotton production in Arkansas. As usual, price volatility in 2023 added another level of difficulty in the quest for being profitable. Cotton futures traded between 80 and 90 cents per pound in 2023, with brief times below 80 cents and above 90 cents. In April, cotton futures dropped below 80 cents per pound, the lowest level in almost four months. This was due to an increase in certificated stocks, declining demand, and a growing world carryover. The average cotton price received by U.S. growers …

Agronomic And Economic Productivity Of Summer Annual Forage Systems Under Different Poultry Litter Application Methods, Ivanelle Meyer, Michael P. Popp, Christine C. Nieman, Amanda J. Ashworth, Phillip R. Owens

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Poultry litter (litter) is a nutrient dense fertilizer that increases nutritive value and yield in pastures in the mid-southern US. Nutrient losses due to runoff and nitrogen volatilization are common when broadcasting litter. As such, incorporating litter below the soil surface (subsurface) was evaluated in comparison to broadcasting in 2021 and 2022 by quantifying yield and nutritive value of annual forages. The study was a randomized complete block design with three forage treatments—sorghum-sudangrass only (Sorghum bicolor L.), cowpea only (Vigna unguiculata L.), and their mixture, and three litter application methods (broadcast, subsurface, and a no litter control). Litter …

Soil Profile Distribution Of Nutrients In Contrasting Soils Amended With Struvite And Other Conventional Phosphorus Fertilizers, Tatum Simms, Kristofor R. Brye, Trenton L. Roberts, Lauren F. Greenlee

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Phosphorus (P) can be recovered from wastewater and used as an alternative fertilizer, namely, struvite [MgNH₄PO₄·6(H₂O)]. However, the soil mobility of wastewater-derived P and other nutrients needs to be evaluated. The objective of this study was to compare the vertical distribution of water-soluble (WS) soil P and other nutrients from a synthetic-wastewater-derived electrochemically precipitated struvite (ECST) to that from a chemically precipitated struvite (CPST), triple superphosphate (TSP), monoammonium phosphate (MAP), and a control in six soils from Arkansas (AR; loam [L] and silt loam [SiL]), Missouri (MO; SiL 1 and SiL 2), and Nebraska …

A Review Of The Interaction Between Potassium Nutrition And Plant Disease Control, C. C. Ortel, T. L. Roberts, J. C. Rupe

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Potassium (K) is an essential macronutrient involved in many physiological and biochemical functions that affect a plant's susceptibility to disease. These processes include stomatal regulation, enzyme activation, and solute transport, each of which is often discussed through the lens of either plant nutrition or plant disease control. However, the interaction between these stresses and the resulting physiological and agronomic impact is an important consideration when managing a cropping system as a whole and is scarcely addressed in the literature. Therefore, a review of the literature was focused on the interaction of K nutrition and the resulting impact on plant disease …

Weed Control And Rice Response To Microencapsulated Acetochlor And A Fenclorim Seed Treatment On A Clay Soil, Tristen H. Avent, Jason K. Norsworthy

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Recent research has demonstrated the ability of a fenclorim seed treatment to reduce rice (Oryza sativa L.) injury to acetochlor. However, all studies were conducted on silt loam soils and have not evaluated rice tolerance or weed control on clay soils. Experiments were initiated in 2021 and 2022 at the Northeast Research and Extension Center near Keiser, AR, to determine rice response and the effectiveness of delayed-preemergence (DPRE)-applied microencapsulated (ME) acetochlor (1.1, 1.7, and 2.3 lb ai acre⁻¹) when applied to a clay soil with and without a fenclorim seed treatment at 0 or 2.5 lb ai …

Photodegradation Of Microplastics Through Nanomaterials: Insights Into Photocatalysts Modification And Detailed Mechanisms, Yiting Xiao, Yang Tian, Wenbo Xu, Jun Zhu

Biological and Agricultural Engineering Faculty Publications and Presentations

Microplastics (MPs) pose a profound environmental challenge, impacting ecosystems and human health through mechanisms such as bioaccumulation and ecosystem contamination. While traditional water treatment methods can partially remove microplastics, their limitations highlight the need for innovative green approaches like photodegradation to ensure more effective and sustainable removal. This review explores the potential of nanomaterial-enhanced photocatalysts in addressing this issue. Utilizing their unique properties like large surface area and tunable bandgap, nanomaterials significantly improve degradation efficiency. Different strategies for photocatalyst modification to improve photocatalytic performance are thoroughly summarized, with a particular emphasis on element doping and heterojunction construction. Furthermore, this review …

Optimizing Recombinant Cas9 Expression: Insights From E. Coli Bl21(De3) Strains For Enhanced Protein Purification And Genome Editing, Shilpi Agrawal, Made Harumi Padmaswari, Abbey L. Stokes, Daniel Maxenberger, Morgan Reese, Adila Khalil, Christopher E. Nelson

Biomedical Engineering Faculty Publications and Presentations

The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, while CRISPR-Cas9 presents notable benefits and encouraging outcomes as a molecular tool and a potential therapeutic agent, the process of producing and purifying recombinant Cas9 protein remains a formidable hurdle. In this study, we systematically investigated the expression of recombinant SpCas9-His in four distinct Escherichia coli (E. coli) strains (Rosetta2, BL21(DE3), BL21(DE3)-pLysS, and BL21(DE3)-Star). Through optimization of culture conditions, including temperature and …

Scaffold-Free Development Of Multicellular Tumor Spheroids With Spatial Characterization Of Structure And Metabolic Radial Profiles, Shelby N. Bess, Gaven K. Smart, Matthew J. Igoe, Timothy J. Muldoon

Biomedical Engineering Faculty Publications and Presentations

Purpose

In vitro assays are essential for studying cellular biology, but traditional monolayer cultures fail to replicate the complex three-dimensional (3D) interactions of cells in living organisms. 3D culture systems offer a more accurate reflection of the cellular microenvironment. However, 3D cultures require robust and unique methods of characterization.

Methods

The goal of this study was to create a 3D spheroid model using cancer cells and macrophages, and to demonstrate a custom image analysis program to assess structural and metabolic changes across spheroid microregions.

Results

Structural characterization shows that cells at the necrotic core show high normalized fluorescence intensities of …

Toxic Love: Mandating Standards In Arkansas’S Domestic Violence Laws, Alia B. Reddell

Arkansas Law Review

This Comment addresses the current scheme of domestic violence statutes, highlighting the inadequate state of domestic violence remedies and the ineffective law enforcement guidelines currently in place; it argues that the Arkansas State Legislature is in need of reforming its domestic violence policies. This recommendation is three-fold and proposes that Arkansas should abandon its current discretionary regime and adopt: (1) mandatory arrest policies; (2) statutory post-arrest procedures; and (3) mandatory prosecutorial policies to effectively protect domestic violence survivors. Part I will discuss the various types of domestic violence arrest statutes. Further, this Part will briefly highlight the significance of an …

A Simple Sandwich Electrochemical Immunosensor For Rapid Detection Of The Alzheimer’S Disease Biomarker Tau Protein, Mingzhu Yang, Yihong Chen, Hongyu Sun, Dujuan Li, Yanbin Li

Biological and Agricultural Engineering Faculty Publications and Presentations

As a typical biomarker of Alzheimer’s disease, rapid and specific detection of tau protein can help improve the early diagnosis and prognosis of the disease. In this study, a simple sandwich electrochemical immunosensor was developed for rapid detection of tau protein. Primary monoclonal antibodies (mAb1) against the middle domain of tau protein (amino acids 189–195) were immobilized on the gold electrode surface through a self-assembled monolayer (SAM) of 3,3′-dithiobis (sulfosuccinimidyl propionate) (DTSSP). Then the tau protein was captured through the specific adsorption between the antigen and the antibody, resulting in a change in the impedance. Secondary monoclonal antibodies (mAb2) against …

Investigating The Relationship Between Hypoxia, Hypoxia-Inducible Factor 1, And The Optical Redox Ratio In Response To Radiation Therapy, Jesse D. Ivers, Nagavenkatasai Puvvada, Charles M. Quick, Narasimhan Rajaram

Biomedical Engineering Faculty Publications and Presentations

Significance

Radiation resistance is a major contributor to cancer treatment failure and is likely driven by multiple pathways. Multivariate visualization that preserves the spatial co-localization of factors could aid in understanding mechanisms of resistance and identifying biomarkers of response.

Aim

We aim to investigate the spatial and temporal relationship between hypoxia, hypoxia-inducible factor 1 (HIF-1α), and metabolism in response to radiation therapy in two cell lines of known radiation resistance and sensitivity.

Approach

Two-photon excited fluorescence and fluorescence lifetime imaging microscopy were used to quantify the optical redox ratio (ORR) and NAD(P)H fluorescent lifetime and bound fraction in frozen tumor …

How Do Demographic Characteristics Of Homeschooling Households Influence The Way Homeschooling Is Practiced?, Albert Cheng, Angela Watson

Education Reform Faculty and Graduate Students Publications

The legitimization of homeschooling during the COVID-19 pandemic and the emergence of new schooling models (i.e., hybrid homeschooling) potentially change the composition of homeschooling families and the way homeschooling is practiced. This study establishes pre-pandemic trends of the demographic composition of U.S. households who homeschool their children. This study also articulates a theory that the way families practice homeschooling is based on practical constraints imposed by household demographic characteristics and, to a lesser extent ideological reasons. These theories are empirically tested using the 2012, 2016, and 2019 waves of the National Household Education Survey and a sample of 1,468 homeschooling …

Revealing Microbial Community Composition And Disinfection Efficacy Evaluation In Wholesale Pork Markets Through 16s Rdna Sequencing, Miao He, Lingyan Ma, Wentao Lv, Yingping Xiao, Hua Yang, Xiofan Wang, Jiangchao Zhao, Hangzhen Lan, Wen Wang, Xingning Xiao

Animal Science Faculty Publications and Presentations

This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p <  .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p <  .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection …

Flexible Biosensors For Food Pathogen Detection, Sonatan Biswas, Md Shariful Islam, Fei Jia, Yunteng Cao, Yanbin Li, Changyong Cao

Biological and Agricultural Engineering Faculty Publications and Presentations

Food contamination poses a significant threat to public health, the economy, and human health worldwide, occurring at any stage of the food supply chain, from farm to fork. Efficient and effective real-time monitoring methods for the early identification and rapid detection of pathogen contamination are critical to preventing possible food safety issues. In the past decade, flexible electrochemical biosensors have rapidly expanded in the detection of foodborne pathogens, owing to their ability to function well at biological interfaces that may be soft, intrinsically curvy, irregular, or deformable. The most important features of flexible sensors are their flexibility, multifunctionality, low cost, …

Effects Of Fast And Slow-Wilting Soybean Genotypes On Fall Armyworm (Spodoptera Frugiperda) Growth And Development, Jessica Ayala, Alejandro Vasquez, Devi Balakrishnan, Evelyn Madrigal, Justin George, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Soybean (Glycine max) is the most important plant protein source, and Fall Armyworm (FAW, Spodoptera frugiperda) is considered a major pest. This study aimed to examine the impact of FAW feeding on soybean accessions that vary in their water use efficiency (WUE) traits, by examining FAW growth and life history parameters along with plant growth response to pest damage. Soybean accessions were grown in a greenhouse and exposed to FAW larval feeding for 48 h at three different soybean growth stages: V3, R3, and R6. The growth and development of the FAW and soybeans were monitored. Results showed that faster …

Life Being Experienced, Amelia Hirsch

Student Literary Anthologies

No abstract provided.

Subject Ninety-Four, Sophie Trist

Student Literary Anthologies

No abstract provided.

Global-To-Local Dependencies In Phosphorus Mass Flows And Markets: Pathways To Improving System Resiliency In Response To Exogenous Shocks, Justin Baker, Nathan Schunk, Matt Scholtz, Ashton Merck, Rebecca Muenich, Paul Westerhoff, James J. Elser, Owen W. Duckworth, Luke Gatiboni, Minhazul Islam, Anna-Maria Marshall, Rosangela Sozzani, Brooke K. Meyer

Biological and Agricultural Engineering Faculty Publications and Presentations

Uneven global distribution of phosphate rock deposits and the supply chains to transport phosphorus (P) make P fertilizers vulnerable to exogenous shocks, including commodity market shocks; extreme weather events or natural disasters; and geopolitical instability, such as trade disputes, disruption of shipping routes, and war. Understanding bidirectional risk transmission (global-to-local and local-to-global) in P supply and consumption chains is thus essential. Ignoring P system interdependencies and associated risks could have major impacts on critical infrastructure operations and increase the vulnerability of global food systems. We highlight recent unanticipated events and cascading effects that have impacted P markets globally. We discuss …

Contributors, Diamond Line Editors

Diamond Line Undergraduate Literary Magazine

No abstract provided.