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Expression Of Ha1 Fragment Of H1n1 Swine Influenza Viral Protein, Hemagglutinin, On The Surface Of Yeast Saccharomyces Cerevisiae Using Pyd5 Shuttle Vector, Ismael Mojica

Biological and Agricultural Engineering Undergraduate Honors Theses

Influenza viruses are enveloped viruses with segmented RNA genome surrounded by a helical symmetry shell. Due to genetic reassortment between avian, swine, and human influenza viruses, a global pandemic may emerge, calling for new methods of mass vaccine production. Yeast is an ideal organism to express viral antigens (e.g. hemagglutinin) because of its natural adjuvant activity, making the expressed proteins more immunogenic when administered to the human body [1,2]. In addition to the purpose of vaccination, yeast is economically convenient by expressing proteins in a fast, inexpensive manner [3,4]. In this study, the goal is to express the HA1 fragment …

Phosphorus Flux From Bottom Sediments In New Spiro Lake, Oklahoma, Katherine Smith

Biological and Agricultural Engineering Undergraduate Honors Theses

Phosphorus loads to water bodies consist of external flux from the watershed and internal flux from the bottom sediments. In this study, the specific objectives were to measure the internal phosphorus flux from bottom sediments under aerobic and anaerobic conditions and determine the effectiveness of various rates of alum treatment on sediment phosphorus release. Eight intact sediment cores from New Spiro Lake were incubated under aerobic and anaerobic conditions for 41 days at room temperature. The cores were treated in the middle of incubation with alum (aluminum sulfate) and sodium aluminate at a ratio of 2:1, ranging from 0.05 to …

The Study Of The Formation Of Oligomers And Amyloid Plaques From Amylin By Capillary Electrophoresis And Fluorescent Microchip Electrophoresis, Shane Weindel

Biological and Agricultural Engineering Undergraduate Honors Theses

Amylin, a pancreatic β-cell hormone, was the focus of this research project. This hormone is co-localized and co-secreted with insulin in response to nutrient stimuli. The hormone inhibits food intake, gastric emptying and glucagon secretion. Insulin and amylin appear to complement each other in the control of plasma glucose levels. Human amylin has a propensity to self-aggregate and to form insoluble bodies. In type 2 diabetes, amylin forms pancreatic islet amyloid plaques, which are found in 90% of people with the disease. It is still uncertain whether or not the deposition of amylin is a primary, or a secondary, event …

The Effects Of Algae Pre-Treatment On The Biomethane Potential Of Swine Wastewater, Joe Barrett Carter

Biological and Agricultural Engineering Undergraduate Honors Theses

Anaerobic digestion is a common method of waste treatment in the agro-industrial and municipal sectors, which utilizes microbial metabolisms that take place in an environment closed to the atmosphere to convert the organic content of wastewater into gas composed of approximately 65% methane and 35% carbon dioxide. This gas can be used as a combustible fuel for the production of heat and/or electricity. Anaerobic digestion is not typically used to treat swine waste because of its low carbon to nitrogen ratio being below the ideal range of 20-30:1. This high nitrogen content of swine waste results in ammonia inhibition of …

Antimicrobial Effects Of Pine Essential Oil Against Listeria Monocytogenes, Elizabeth Louise Marhefka

Biological and Agricultural Engineering Undergraduate Honors Theses

Short leaf and loblolly pine were harvested in May 2014 and June 2014, respectively, at the University of Arkansas Monticello in Monticello, Arkansas. Short leaf and loblolly essential oils were prepared by hydrodistillation, using a Clevenger apparatus. Essential oils were extracted at separate times and showed different concentrations of the components α-pinene, β- pinene, 3-carene, limonene, and terpineol. Pine essential oil samples were tested through disc diffusion assay for antimicrobial activity against Listeria monocytogenes. This was done by placing ten μL of each essential oil sample on a paper disc on an agar plate that was inoculated with L. monocytogenes …

Comparison Of Microwear On Rodent Molars From Differing Species And A Wide Range Of Environments, Mikko Joiner

Anthropology Undergraduate Honors Theses

Dental microwear analysis is a very useful tool when trying to infer the diet of a particular organism. By studying the use-wear scars left on the enamel of the tooth due to eaten objects, one can infer the diet of the organism because certain types of food leave certain types of scars. For example, the consumption of tree parts produces pits, while the consumption of grasses produces striated scratches (Ungar et al., 2007). Thus, based on the type of microwear, the diet of the organism in question can be deduced, which indicates the type of environment that it lives in. …

"V Is For Violin" - An Original Playscript, Brittany Taylor

Graduate Theses and Dissertations

As and MFA playwrighting candidate, I was given the opportunity to witness a fully realized production of my work. For this academic opportunity I chose the "V is for Violin." I will discuss the genesis of the idea, the evolution of the play, research conducted, and what I learned in the production process of the show.

Predicting The Future: Our Food System In 2025, Susan A. Schneider

Journal of Food Law & Policy

It has been inspiring participate in the development of food law and policy as a recognized discipline. Over the last ten years, the Journal of Food Law & Policy and the LL.M. Program in Agricultural & Food Law have each played a significant role in that development. And, the landscape continues to evolve. The impact of changing attitudes toward our food system, environmental challenges, public health concerns and other integrated influences are certain to cause a continued evolution in our food system and the legal system that frames it. My task with this essay is to consider our food system …

Journal Of Food Law & Policy - Spring 2015, Journal Editors

Journal of Food Law & Policy

No abstract provided.

Methanogen Metabolism In The Presence Of Iron Compounds: A Martian Environment Simulation, Monroe Phillip Cowan

Chemistry & Biochemistry Undergraduate Honors Theses

The climate on the face of Mars is hostile towards life. Thus, any organisms potentially living on Mars would likely inhabit a subsurface environment, in which conditions are wetter and warmer. However, organisms living beneath the surface would be significantly deprived of light and some organic compounds necessary for the sustenance of most life. The discovery of methane gas in Mars’ atmosphere has led to speculation that methanogens, obligate anaerobes which produce methane as waste, are the organisms most likely to survive in a subsurface environment. These members of domain Archaea subsist on water, hydrogen, and carbon dioxide. In addition, …

Opportunity, Temporal Patterns, And Successful Outcomes Of Far-Right Terrorism Incidents In The United States, Brent Ryan Klein

Graduate Theses and Dissertations

This thesis examines the impact that far-right terrorism opportunity structures and temporal patterns of precursor activity have on incident outcomes. Data from the American Terrorism Study (ATS) are extracted for several attributes of far-right opportunity, in addition to measures for temporal patterns of planning and preparatory behaviors. Bivariate and multivariate findings generally support expectations that target attractiveness and vulnerability, far-right group structures, and patterns of precursor activity are significantly associated with incident outcomes. The paper concludes with suggestions for future research and several implications for homeland security policy.

A Descriptive Study Of The Use Of Neurofeedback In Counseling, Khwlah Jamil Al-Ansari

Graduate Theses and Dissertations

This study examines neuro-counseling in the mental health field and the concept of neurofeedback training (NFB) as an intervention in the counseling profession. Numbers of studies have indicated that NFB may be effective in the field of counseling. The purpose of this survey study is to explore the use of neurofeedback in the field of counseling.

Additionally, the study used a quantitative descriptive survey research method and descriptive statistics. The survey was sent to members of the American Counseling Association (ACA), and 93 professionals participated. The majority of the sample was licensed professional counselors, professionals with a degree in counseling, …

The Role Of Stress: Low Birth Weight And Preterm Birth For African American Women, Tionna Latrice Jenkins

Graduate Theses and Dissertations

This population-based study evaluates the impact that psychoSocial stress has on adverse birth outcomes of low birth weight (LBW) and pre-term birth (PTB) among African American mothers in Arkansas. The relationship between adverse birth outcomes in African American women and stress in comparison to non-Hispanic Caucasian women data was evaluated from the Pregnancy Risk Assessment Monitoring System (PRAMS) quantitative survey. Data from 2005 through 2010 was reviewed to show the impact that psychoSocial stress has on adverse birth outcomes. The study sample was comprised of 14,196 participants.

Ethnic group status is the key maternal-level independent variable in this study. Of …

Management Of A Primordial Problem: Redox-Sensitive Transcriptional Regulation In Methanosarcina Acetivorans, Ryan Christopher Sheehan

Graduate Theses and Dissertations

The primordial Earth which hosted the first forms of life was an environment free of oxygen. Early organisms utilized metabolisms dependent upon anaerobic conditions and incorporated systems to which oxygen is deleterious. As the content of oxygen in Earth's atmosphere increased, anaerobic organisms had to acquire methods to sense and combat oxygen and reactive oxygen species. Several mechanisms were advantageous to such anaerobic organisms which correlated transcriptional processes with the redox state of the cell so that energy may be conserved and oxygen stress recovery genes activated during periods of oxidative stress. Iron sulfur (Fe-S) cluster cofactors incorporated within RNA …

Defining Primary And Academic Discourse Through Instructional Methods In A Single Junior High Classroom, Ashley Nicole Gerhardson

Graduate Theses and Dissertations

In this study, I examine the use of academic discourse in the school setting and discuss the relation of the primary discourse to academic discourse. I focus on the verbal exchanges between a teacher and her students as these students acquire the academic discourse of the English classroom. The study focuses on the ideas of primary discourse and secondary or academic discourse as presented by Gee (1996) and focuses on his idea of Social languages.

Using a microethnographic study, I develop the idea of how the teacher related to her students and how a single educator felt about the purpose …

Impacts Of Micrornas On Skeletal Muscle Protein Synthesis And Mitochondrial Quality, David Lee

Graduate Theses and Dissertations

microRNA (miRNA) post-transcriptional modification is becoming a well-established mechanism for controlling mRNA translation. microRNAs -1, -133, and -206 are under the control of skeletal muscle promoters and affect muscle plasticity and metabolic health. A detailed review on the generation and processing of miRNAs with a view to skeletal muscle brings up intriguing connections in the transcriptional connections between multiple miRNAs. Additionally, exciting new research has defined a role of miRNAs in skeletal muscle mitochondria showing an additional, direct link to metabolic function. Multiple investigations in models of exercise, aging, hypertrophy, and injury have shown how these interventions can affect miRNA …

A Phylogenetic Analysis Of Dictyostelium Purpureum Based On Nuclear Rdna Sequences, Mahmoud Suliman

Graduate Theses and Dissertations

Dictyostelids (cellular slime molds) are eukaryotic microorganisms that have both unicellular and multicellular stages during their life cycle. In this study, a molecular phylogenetic analysis was conducted for isolates of one species (Dictyostelium purpureum) based DNA sequences of the ITS, 5.8S and SSU regions of nuclear rDNA. Moreover, a detailed morphological study was carried out using images obtained with both dissecting and compound microscopes. Mating experiments were carried out to assess macrocysts formation between each pair of isolates. The constructed molecular phylogenetic trees indicate that (1) D. purpureum isolates are more closely related to each other than to other species …

Controls On Dissolution Rate Variation At A Pair Of Underflow-Overflow Springs At The Savoy Experimental Watershed, Kiefer Allen Vaughn

Graduate Theses and Dissertations

Physical dissolution experiments and numerical modeling have been used in the past to study limestone dissolution rates. Numerical models have typically used constant dissolution rates, whereas rates in nature vary in time. Limestone tablets allow natural estimation of rates over month time scales, but these rates cannot necessarily be extrapolated to geologic timescales and also do not aid our understanding of short term variability. This study characterizes natural variability in these rates and examines potential causes of that variability from first principles. This may enable more accurate projections of dissolution rates within models. This study combines measurement of physical and …

Mapping Ancient Baldcypress Forests For Conservation At Black River, North Carolina, Jordan Nichole Burns

Graduate Theses and Dissertations

A few ancient baldcypress-bottomland hardwood forests survive across the southeastern United States in a mosaic of remnant old-growth stands left untouched by extensive logging during the early 20th century. Uncut stands in the Southeast that survived centuries of disturbance following European settlement tended to be too senescent and non-commercial to justify logging. Remnant ancient baldcypress forests at Black River, North Carolina, appear to contain the oldest living trees in eastern North America and The Nature Conservancy has protected several of these stands. However, the full extent of ancient bottomland forests along Black River is not known and many valuable tracts …

An Examination Of Psychoeducation And Its Potential Modifying Influence On Alcohol Use Patterns Among Adults Reporting Co-Occurring Posttraumatic Stress Symptoms And Hazardous Alcohol Consumption, Sarah Jo Bujarski

Graduate Theses and Dissertations

Research has suggested that consumption of alcohol in the presence of elevated posttraumatic stress symptom (PTSS) may serve an avoidant function to cope with negative emotions. These coping-related motives for use are theorized to both maintain PTSS and relate to poorer prognoses in treatment for alcohol use disorders (AUDs). Treatments utilizing coping skills training, which typically also involves educating clients about the negative consequences of drinking alcohol to cope, suggest the utility of targeting coping behaviors to reduce alcohol use. These studies, however, have not attempted to isolate the effects of psychoeducation on alcohol-related factors. The current study investigated the …

The Paleoepidemiology Of Malaria In The Ancient Near East, Nicole Elizabeth Smith

Graduate Theses and Dissertations

The end of the Late Bronze Age in the Near East (1300 - 1200 BCE) saw the widespread collapse of several large cultural centers, the reasons for which are a subject of continued debate. Evidence from events leading up to this cultural collapse suggest epidemic disease may have factored into the eventual downfall of these early civilizations. Recent DNA analysis from Egyptian mummies who lived during the period leading up to the Late Bronze Age collapse identified malaria in several elite individuals, suggesting the widespread prevalence of this infectious disease in Egypt. However, the exact prevalence, antiquity, and dynamics of …

Nutrient Management For Growing Dandelion (Taraxacum Officinale L.) In Nutrient Film And Deep Flow Hydroponics, Reetinder Gill

Graduate Theses and Dissertations

This research study was conducted to determine an optimal nutrient strategy for dandelion production in nutrient film technique (NFT) and deep flow technique (DFT) systems of hydroponics. It was achieved by growing dandelion at varying levels of nutrient solution concentration and pH in both NFT and DFT systems. Additionally, an optimal nutrient solution concentration and timing of application for dandelion seedling production was also determined.

Inducing And Characterizing M2c Macrophages At A Non-Degradable Implant In Vivo, Geoffrey David Keeler

Graduate Theses and Dissertations

The implantation of biomaterials in the body leads to a reaction known as the foreign body response (FBR) which leads to the eventual encapsulation of the biomaterial. This encapsulation proves to be detrimental to certain biomaterials, such as implanted glucose sensors, which rely on interacting with the extracellular space for proper function. Altering the FBR has become of interest in an effort to increase the longevity and integration of biomaterials. One strategy for altering the FBR is by targeting the macrophage, shown to play an important role in the FBR. In this work, the microdialysis sampling technique was used to …

Influence Of A Potentially Destabilizing Central Tryptophan On Transmembrane Helix Domains, Vasupradha Suresh Kumar

Chemistry & Biochemistry Undergraduate Honors Theses

The vital role that membrane proteins play in cell mechanisms and the inner workings of disease in our bodies has been universally accepted. Thus, the study of protein-membrane and transmembrane interactions has become increasingly significant in understanding these membrane proteins. Synthetic model peptides, such as GWALP23 (acetyl-GGALW5LALALALALALALW19LAGA-amide)—designed at the University of Arkansas and composed of the amino acid residues of glycine (G), alanine (A), leucine (L) and tryptophan (W) —provide a favorable “host” framework for investigations of the influence of chosen “guest” amino acids. For example, it is of interest to know the consequences of having a third, centrally located, …

Chymotrypsin Digestion Analysis To Characterize Site-Specific Incorporation Of An Extrinsic Fluorescent Probe On A Ras-Related Protein., Akash D. Patel

Chemistry & Biochemistry Undergraduate Honors Theses

Ras Homolog Enriched in Brain (RHEB) is a member of Ras GTPase family and plays an important role in regulation of cell growth and cell cycle proliferation. RHEB is regulated by cycling between active (GTP-bound) and inactive (GDP-bound) state. Guanidine Nucleotide Exchange Factors (GEFs) and GTPase-activationg proteins (GAPs) are the key regulators of RHEB, and it is of interest to closely study their interactions with RHEB. Towards that end, this project was designed to label RHEB with an environmentally sensitive extrinsic fluorophore, succinimidyl 6-(N-(7-nitrobenz-2-oxa-1.3-diazol-4-yl)amino)hexanoate (sNBD), to be used to monitor RHEB-protein interactions. Due to its extreme sensitivity to the surrounding …

Increased Microdialysis Recovery Of Large Molecular Weight Analytes Via Ultrafiltration, Michael C. Elkins

Chemistry & Biochemistry Undergraduate Honors Theses

Microdialysis is a sampling method based on the passive diffusion of solutes across a semi-permeable hollow-fiber membrane that is driven by a concentration gradient. The membrane has a defined molecular weight cutoff, which causes larger molecular solutes such as proteins with molecular weights of approximately 8-80 kDa to have low recoveries. The purpose of this research is to utilize ultrafiltration across the membrane through push-pull and vacuum ultrafiltration methods as a means to increase recovery of large molecular weight analyte. These experiments were carried out using Methyl Orange (MO), Fluorescein Isothiocyanate 4 kDa (FITC-4), and Fluorescein Isothiocyanate 40 kDa (FITC-40). …

Developing Graphene-Based Supercapacitors For High Temperature Applications, Lea Rachel Rogers

Chemistry & Biochemistry Undergraduate Honors Theses

Supercapacitors are ideal tools for energy storage due to their wide range of operation temperatures, long cycle-lives, high power densities of up to 13,000 W/kg, and fast rates of charge-discharge thereby having a high specific capacitance. This research reports synthesis of grapheme-based materials using a modified Hummer’s method. Graphene oxide synthesized first and then converted to reduced graphene oxide sheets and graphene-powder, which both increase the ionic conductivity due to sp2 bonding and a high surface area. These materials were then constructed into a button-cell supercapacitor for testing. For the first supercapacitors, a porous polypropylene membrane (Celgard) that acted as …

Design Of Antimicrobial Peptide Based On The Heparin Binding Segment Of Fgf-1, Alice Margaret Power

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast Growth Factors (FGFs) are heparin-binding proteins known for their involvement in various biological processes such as cell differentiation and wound healing.1 The heparin binding site of FGF-1 displays a unique stretch of positive amino acid sequence and facilitates a very strong binding to negatively charged heparin due to the formation of electrostatic interaction. The aim of this study is to analyze and modify the novel antimicrobial peptide sequence (GST-HB) designed based on the heparin-binding region of FGF-1 as well as other polycationic microbial sequences. These aspects will be examined using various experimental techniques including overexpression of GST-HB, purification using …

Additional Sex Combs-Like 2 (Asxl2): A Psychological Stress-Related Gene In A Chicken Stress Model, Emily Sandra Jacobson

Chemistry & Biochemistry Undergraduate Honors Theses

Chronic stress is a problem not only in terms of its psychological effects but also due to the harmful physiological changes induced in the individual. Although chronic stress is recognized as detrimental and rampant throughout society, the stress system itself remains poorly understood in terms of comprehending which genes are involved in regulating and carrying out stress-induced signals. In a previous study, 57 candidate genes in the stress pathway had been identified based on the presence of stress-associated single nucleotide polymorphisms (SNPs) and differential expression from microarray data. The objective of this study was to investigate Additional Sex Combs-Like 2 …

Devlopment Toward Fluorescence Imaging Of Microdialysis Sampling Diffusion Profiles, Nicholas Edward Baioni

Chemistry & Biochemistry Undergraduate Honors Theses

Microdialysis is a diffusion-based method used to collect analytes from specific locations in the body. Matrix Metalloproteinases (MMPs) are important enzymes located throughout the body that play a role in the breakdown of extracellular matrix. Microdialysis sampling in conjunction with appropriate analysis methods can be used to observe the reactions of MMPs in real time. This project is aimed at determining the activity of porcine pancreatic enzyme, a known substitute for MMP-12, using nanodrop spectroscopy. This is a necessary pre-requisite toward using microdialysis in an in vivo imaging setup. These experiments were performed using Succinyl(Ala)_3-p-nitroanilide (suc(Ala)3-p-NA), 4-nitroaniline, and porcine pancreatic …