Digital Commons Network^™

Effects Of Filtration Conditions On Clearance Of Bacteriophage, Rigen Te

Graduate Theses and Dissertations

Virus Clearance is one of the major challenges in biopharmaceutical industry, especially in the manufacturing of drugs. U.S. Food and Drug Administration (FDA) recommends using several model bacteriophages as surrogates of pathogenic mammalian virus for process validation. In this work, two bacteriophages were tested against 30 kDa, 100 kDa, 300 kDa polyethersulfone (PES) membranes to investigate the effects of pore size on virus clearance. Virus particles were spiked into the protein feed solutions containing bovine serum albumin (BSA) or lysozyme at different concentrations. Besides protein concentration, the effects of feed pH on the filtration performance and virus rejection were also …

Supplemental Trace Minerals (Zn, Cu, And Mn) As Sulfates Or Hydroxy Trace Mineral Sources For Beef Heifers, Randy Hunter Burnett

Graduate Theses and Dissertations

Crossbred heifers (n = 286, 255 ± 4.5 kg initial BW, 295 ± 16.5 d of age) were used over a 2-yr period to determine the effects of mineral source on beef heifer development at 2 locations (n = 71 and n = 72, Fayetteville, blocks 1 and 4; n = 72 in each of 2 breeding groups, Batesville, blocks 2 and 3). Heifers were stratified based on initial BW, age, health, prior research projects, and sire, and then assigned to 6 groups of 12 heifers, that were assigned randomly to 1 of 2 trace mineral treatments. The 2 treatments …

A Comparative Analysis Of Feed And Environmental Factors On Broiler Growth In The United States, Alexandra Gulli

Graduate Theses and Dissertations

Broiler production in the United States has become an important industry within the last half century. As the demand for poultry continues to increase and the concern of climate change also increases, it will become an important part of the industry to become more sustainable. This study uses a feed optimization model, a broiler growth model, and a life cycle assessment (LCA) to determine how feed ingredients and the barn environment can affect the broiler’s growth and production emissions. A multi-criteria ration optimization model is used to produce feeds based on carbon footprint and cost. A growth model is used …

Phylogenetic Diversity Of The Bacterial Communities In Craft Beer, Lindsey Rodhouse

Graduate Theses and Dissertations

The craft beer industry is increasing in popularity in the United States. The craft brewing process typically does not use a pasteurization step, therefore the boiling process is the primary critical control step. Any microorganisms introduced after boiling, or those that are not killed during boiling, are likely to participate in fermentation and persist in the final product. Previous culture-based studies have isolated bacteria and yeast from craft beers at specific time points, but little research has been done on the process as a whole. The objectives of this research are to (1) track bacteria development throughout the brewing process …

Membrane Surface Engineering For Biochemical Applications, Anh T. Vu

Graduate Theses and Dissertations

Synthetic membranes have been frequently used for many fields, such as, the food and beverage, biopharmaceutical, and biofuel industries. In the beer industry, microfiltration frequently suffers from fouling due to the interaction between different species. It is shown that polyphenols can form cross-links with protein molecules, forming insoluble aggregates. However, by adding an optimal amount of polysaccharides these aggregates can be disrupted thus reducing fouling by the aggregates. Confocal laser scanning microscopy (CLSM) is a powerful technique to locate the foulants inside the wet membrane in order to understand more about the behavior of fouling in microfiltration.

Membrane surface modification …

Natural Air Drying And Storage Of Soybean Seed And Implications On Germination Rates, Vigor, And Oil Quality, Zachary Young

Graduate Theses and Dissertations

Recently introduced technology-comprising cables used for monitoring grain moisture content (MC) and temperature throughout the entire grain mass during drying offers a means to utilize low-temperature natural air-drying for soybean seed. From an electronic monitor and fan control standpoint, the new technology appears to be very promising for managing drying and storage conditions for soybean seed. The objectives for this study were to simulate conditions typically encountered in on-farm, in-bin drying systems and investigate impact of seed cultivar, temperature, moisture content and duration of storage on seed germination rates and vigor (electrical conductivity). In addition this research addresses the problem …

Using Stable Isotope Geochemistry To Determine Changing Paleohydrology And Diagenetic Alteration In The Late Cretaceous Kaiparowits Formation, Ut Usa, Daigo Yamamura

Graduate Theses and Dissertations

The Western Interior Basin of the North America preserves one of the best sedimentary and paleontological records of the Cretaceous in the world. The Upper Cretaceous Kaiparowits Formation is a rapidly deposited fluvial sequence and preserves one of the most complete terrestrial fossil record of the North America. Such a unique deposit provides an opportunity to investigate the interaction between the physical environment and ecology. In an effort to decipher such interaction, stable isotope composition of cements in sedimentary rocks, concretions and vertebrate fossils were analyzed.

Despite the difference in facies and sedimentary architecture, the isotope composition does not change …

The Parent/Teacher Relationship And The Effectiveness Of The Teaching Important Parenting Skills (Tips) Program For Enhancing Parents' Knowledge About Child Development, Meghan Anderson

Graduate Theses and Dissertations

Parent involvement in education has been highly encouraged because of its strong associations with positive developmental outcomes for children. Teaching Important Parenting Skills: TIPS for Great Kids! (TIPS) is a program in Arkansas that relays research-based information cards to parents through the support network of the teachers. This study examines how effective the TIPS program was in enhancing parent’s knowledge on child development and observes the influence of the parent-teacher relationship. Although no significant correlation was found between the parent/teacher relationship and parent knowledge, a significant correlation was discovered between the parent/teacher relationship and whether parents read the TIPS card …

A Numerical Study Of The Interaction Between One Dimensional Carbyne Chain And Single Stranded Dna, Zeina Salman

Graduate Theses and Dissertations

resolution at the single nucleotide level when developing DNA sequencers. The purpose of this research was to numerically study the electrical properties associated with the interaction between 1D carbon chain, known as carbyne, and ssDNA. First, the electrical properties of the carbyne chain were calculated. Second, the electrical properties of the carbyne chain were calculated in the presence of different ssDNA bases. Analyzing the differences between the two cases led to determining the effects of these different bases on the electrical properties. The numerical simulation approach conducted in this research was based on the first-principle simulation. The first-principle simulation was …

Noninvasive Measures Of Stress And Lameness In Broilers, Shawna L. Weimer

Graduate Theses and Dissertations

The concept of broiler chicken welfare has evolved from a component of animal husbandry to a label on a chicken product package. Recent attention from the public has led to the need for higher welfare standards for animal production. A primary concern of broiler production is low activity/locomotion attributed to fast growth rates leading to poor leg health. To collect the scientific data necessary to determine conditions providing the best welfare for the bird, multiple methods of assessment are required. Stress is influenced by external (temperature, humidity, lighting, stocking density) and internal (metabolism, thermoregulation, hormonal balance) factors. This series of …

The University Of Arkansas Institutional Digital Repository Annual Progress Report, July 2017, University Of Arkansas, Fayetteville. Libraries

Institutional Digital Repository Annual Progress Report

Scholarworks@uark : Digital repository at the University of Arkansas.

Annual Report, 2016-2017, University Of Arkansas Fayetteville. J. William Fulbright College Of Arts And Sciences

College of Arts and Sciences Annual Report

No abstract provided.

Annual Report, 2016-2017, University Of Arkansas, Fayetteville. Office Of The Vice Provost For Research And Innovation

Research and Innovation Annual Report

No abstract provided.

Robust Nonparametric Quantification Of Clustering Density Of Molecules In Single-Molecule Localization Microscopy, Shenghang Jiang, Seongjin Park, Sai Divya Challapalli, Jingyi Fei, Yong Wang

Physics Faculty Publications and Presentations

We report a robust nonparametric descriptor, J'(r), for quantifying the density of clustering molecules in single-molecule localization microscopy. J'(r), based on nearest neighbor distribution functions, does not require any parameter as an input for analyzing point patterns. We show that J'(r) displays a valley shape in the presence of clusters of molecules, and the characteristics of the valley reliably report the clustering features in the data. Most importantly, the position of the J'(r) valley ([Formula: see text]) depends exclusively on the density of clustering molecules (ρc). Therefore, it is ideal for direct estimation of the clustering density of molecules in …

Heparin Affinity Tag And Applications Thereof, Thallapuranam Krishnaswamy Suresh Kumar, Srinivas Jayanthi, Jacqueline Morris, Alicia D. Brown, David S. Mcnabb, Ralph Henry

Patents Granted

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids …

Sustainability Assessment Of U.S. Beef Production Systems, Greg Thoma, Ben Putman, Marty Matlock, Jennie Popp, Leah English

Food Systems

With increasing public concern and awareness of agricultural sustainability issues, comprehensive methodologies such as life cycle assessment are required to benchmark the beef industry and identify areas of opportunity for continuous improvement. To that end, the Beef Checkoff completed a retrospective sustainability assessment benchmark in 2013 by using Eco-efficiency analysis to compare the years 2005 and 2011. At the time of the analysis, the methodology used was the most up-to-date and comprehensive – indeed the analysis remains one of the only complete cradle-to-grave assessments of the U.S. beef industry. In 2015, a further refined version of the Eco-efficiency analysis was …

Mole Street Journal, June 2017, University Of Arkansas, Fayetteville. Dept. Of Chemistry And Biochemistry

Mole Street Journal

No abstract provided.

Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther

Horticulture Undergraduate Honors Theses

In 2013, Castro et al., produced the first linkage map for primocane-fruiting blackberries using the cross ‘Prime-Jim®’ x ‘Arapaho.’ This mapping population has been maintained since the original experiment with the hopes of conducting future studies. Further research was determined to be needed on the population to better characterize additional phenotypic traits, and since nine years had passed since the seedlings were established, there was a need to assess the continued genetic purity of the population using molecular methods. Phenotypic data was collected to analyze the variation of the population including soluble solids content, titratable acidity, average weight, shape, and …

Adopting Children With Down Syndrome: A Qualitative Study Of Family Experiences, Emily L. Richter

Rehabilitation, Human Resources and Communication Disorders Undergraduate Honors Theses

The purpose of this study is to qualitatively investigate and compare the family beliefs, values, and perceptions of parenting of families who have adopted children with Down syndrome (DS) to parents who have biological children with DS. Participants in the study included 27 parents of biological children with DS and six parents of adopted children with DS. Participants were interviewed through a digital survey that was distributed through Qualtrics Online Survey Software. Quantitative analysis placed the participants into groups and qualitative analysis was used to identify beliefs, values, and perceptions. Results showed that while both adoptive and biological parents indicated …

An Internationally Adopted Child's Transition: A Family Story, Holly C. Heckmann

Rehabilitation, Human Resources and Communication Disorders Undergraduate Honors Theses

The goal of this study was to contribute to the literature on international adoption by conducting a case study with one adoptive family. Data was collected using a semi-structured, in-depth interview that was audio recorded and transcribed word-for-word. The interview questions asked about family configuration, language background and use, adoptive family decisions about cultural inclusiveness, and the transition from home to school. The analysis was member-checked following coding for the themes that emerged. Results indicated that lingering differences from reduced exposure to language in the first year of life took the form of subtle language differences that continued through the …

Simulating Foodborne Pathogens In Poultry Production And Processing To Defend Against Intentional Contamination, Silas B. Lankford

Computer Science and Computer Engineering Undergraduate Honors Theses

There is a lack of data in recent history of food terrorism attacks, and as such, it is difficult to predict its impact. The food supply industry is one of the most vulnerable industries for terrorist threats while the poultry industry is one of the largest food industries in the United States. A small food terrorism attack against just a single poultry processing center has the potential to affect a much larger population than its immediate consumers. In this work, the spread of foodborne pathogens is simulated in a poultry production and processing system to defend against intentional contamination. An …

Understanding N-Nitrosodimethylamine Formation In Water: Chloramine Chemistry, Kinetics, And A Proposed Reaction Pathway, Huong Thu Pham

Graduate Theses and Dissertations

The formation of N-nitrosodimethylamine (NDMA) in drinking water systems is a concern because of its potential carcinogenicity and occurrence at toxicologically relevant levels. The postulated mechanism for NDMA formation involves a substitution between dichloramine and amine-based precursors to form an unsymmetrical dimethylhydrazine (UDMH), which is then oxidized by ground-state molecular oxygen to form NDMA. However, this latter reaction is spin forbidden, thus likely occurs at a slow rate. It is hypothesized that the reaction between monochloramine and hydroxylamine (a nitrification product) may form an intermediate, which is involved in the NDMA formation pathway. This intermediate may also be generated from …

Total N-Nitrosamine Precursor Adsorption With Carbon Nanotubes: Elucidating Controlling Physiochemical Properties And Developing A Size-Resolved Precursor Surrogate, Erin Needham

Graduate Theses and Dissertations

As drinking water sources become increasingly impaired with nutrients and wastewater treatment plant (WWTP) effluent, formation of disinfection byproducts (DBPs) – such as trihalomethanes (THMs), dihaloacetonitriles (DHANs), and N-nitrosamines – during water treatment may also increase. N-nitrosamines may comprise the bulk of the chronic toxicity in treated drinking waters despite forming at low ng/L levels. This research seeks to elucidate physicochemical properties of carbon nanotubes (CNTs) for removal of DBP precursors, with an emphasis on total N-nitrosamines (TONO).

Batch experiments with CNTs were completed to assess adsorption of THM, DHAN, and TONO precursors; physiochemical properties of CNTs were quantified through …

Development Of A Multiband Remote Sensing System For Determination Of Unsaturated Soil Properties, Cyrus D. Garner

Graduate Theses and Dissertations

A multiband system including active microwave sensing and visible-near infrared reflectance spectroscopy was developed to measure unsaturated soil properties in both field and laboratory environments. Remote measurements of soil volumetric water content (θv), soil water matric potential (ψ), and soil index properties (liquid limit [LL], plastic limit [PL], and clay fraction [CF]) were conducted. Field-based measurement of θv was conducted using a ground-based radar system and field measurements within 10 percentage points of measurements acquired with traditional sampling techniques were obtained. Laboratory-based, visible and near infrared spectroscopy was found to be capable of obtaining empirical, soil specific regression functions (partial …

Trihalomethane, Dihaloacetonitrile, And Total N-Nitrosamine Precursor Adsorption By Carbon Nanotubes: The Importance Of Surface Oxides And Pore Volume, Erin Needham

Graduate Theses and Dissertations

As drinking water sources become increasingly impaired, enhanced removal of natural organic matter (NOM) may be required to curb formation of disinfection byproducts (DBPs) upon chlor(am)ination. While carbon nanotubes (CNTs) can adsorb NOM, their properties for DBP precursor adsorption have not been elucidated. Nine types of CNTs were assessed for trihalomethane (THM), dihaloacetonitrile (DHAN), and total N-nitrosamine (TONO) precursor adsorption. Batch isotherm experiments were completed with lake water and, to simulate an impaired condition, effluent from a wastewater treatment plant (WWTP). Adsorption varied with CNT type and dose, with TONO precursors having the highest percent removals from WWTP effluent (up …

Rock Art Management And Landscape Change: Mixed Field Assessment Techniques For Cultural Stone Decay, Kaelin M. Groom

Graduate Theses and Dissertations

As tourism continues to grow as one of the world’s most ubiquitous markets, the development and promotion of non-invasive techniques for cultural stone decay analysis and landscape change are vital to establishing conditional base-lines to best aid cultural heritage management (CRM) efficacy. Using rock art as a medium, this dissertation presents three independent case studies employing the Rock Art Stability Index (RASI) and repeat photography to explore the merits of mixed rapid field assessment techniques in relation to CRM and heritage tourism. While rock art is only one example of irreplaceable world heritage resources, examining how they decay and what …

Spatial Variability Of Seedling Disease Pressure In Cotton Fields, Kyle Douglas Wilson

Graduate Theses and Dissertations

Seedling diseases are important factors in cotton stand establishment, and seedling disease pathogens are widespread in fields in Arkansas. Little is known about the variability of seedling disease pressure within fields. With expanded adoption of site-specific management and other precision agriculture approaches, cotton producers are increasingly interested in predicting seedling disease pressure, particularly in spatially variable fields. The cotton seedling disease pathogens include the soilborne pathogens Thielaviopsis basicola, Rhizoctonia solani, Pythium spp., and Fusarium spp. These pathogens can survive in soil for long periods and, and when the environment is conducive, these pathogens can act individually or in combination to …

Muscle Oxidative Phenotype In The Progression Of Cancer-Cachexia, Haley N. Mccarver

Health, Human Performance and Recreation Undergraduate Honors Theses

Cancer is one of the leading causes of death across the world. Cancer-cachexia is a serious complication induced by cancer resulting in whole body muscle wasting and is responsible for up to 40% of cancer related deaths. Underlying mechanisms of cancer-cachexia are not well understood; however, a loss in oxidative metabolism in skeletal muscle is apparent in cancer-cachexia. PURPOSE: To examine oxidative phenotype of skeletal muscle in tumor bearing mice during progression of cancer-cachexia. METHODS: Mice were implanted with Lewis Lung Carcinoma (LLC) or sham phosphate buffered saline (PBS) at 8 weeks of age. The tumor was allowed to progress …

Eulogy To Architecture: The Three-Dimensional Collage City Of Nostalgia, Molly A. Evans

Architecture Undergraduate Honors Theses

In our time of existence on the Earth, human beings have designed and realized beautiful things. As we face the challenges that confront us today, we begin to understand the fragility of humankind’s creations. Many of the world’s cities and buildings lie in ruins, gazed at by tourists, studied by scholars, while more lie buried in the ground for hundreds of years, some never to be rediscovered. Everything around us is an accumulation of knowledge and ideas built upon for centuries, now facing questionable circumstances. Of course, the more recent Aleppo and other Middle Eastern cities have fallen subject to …

Optical Imaging Of Metabolic Adaptability As A Biomarker For Metastatic Potential In Breast Cancer Cells, Mason G. Harper

Biomedical Engineering Undergraduate Honors Theses

Breast cancer metastasis is the main cause for mortality in breast cancer patients. However, knowledge of metastatic recurrence is limited, and there is a need to understand metastatic recurrence in order to treat breast cancer patients more effectively. Highly invasive metastatic breast cancer has shown to exhibit metabolic adaptability, transitioning from glycolysis to oxidative phosphorylation in the presence of microenvironmental stress. NADH and FAD are naturally occurring cofactor products during glycolysis and oxidative phosphorylation, respectively, and they are of particular importance during these metabolic processes due to their endogenous fluorescence. Measuring the ratio of fluorescence intensities of these cofactors through …