Digital Commons Network^™

Farmer Cooperatives "Take Cover": The Capper-Volstead Exemption Is Under Siege, Donald M. Barnes, Jay L. Levine

Arkansas Law Review

"When tillage begins, other arts follow. The farmers, therefore, are the founders of human civilization." There can be little dispute that food production is of vital interest to any nation’s security and economy. For this reason, the United States Congress, like many other legislatures around the world, has accorded special treatment to the agricultural industry, and particularly to farmers. One example of this special treatment is the Capper-Volstead Act, which provides farmers with immunity from antitrust liability for joint conduct undertaken by and through an “association” of producers.

The Efficacy Of A Communication Guide On Stress Experienced By Family Members Of Patients Admitted In The Intensive Care Unit With Covid-19, Kandace Williams

The Eleanor Mann School of Nursing DNP Capstone Projects

Coronavirus disease (COVID-19), first introduced in the United States on January 20, 2020, has created worldwide panic due insufficient research and understanding of the nature of this new disease. Patients suffering from COVID-19 often require intensive care unit (ICU) admissions, resulting in stress and confusion amongst patients and their families. The high transmissibility of the disease has caused hospitals to adopt firm visitor restrictions to protect the public from exposure and spread. Families experience increased anxiety and stress due to ineffective communication with staff and restricted access to their hospitalized loved ones. A needs assessment revealed a gap in care …

Are Populations Of Economically Important Bonefish And Queen Conch 'Open' Or 'Closed' In The Northern Caribbean Basin?, Marlis R. Douglas, Tyler Chafin, Julie E. Claussen, David P. Philipp, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Demographics of co-occurring species can often be diagnosed through population genomic analyses of single nucleotide polymorphisms (SNPs). These data can define population structure, gene flow, and candidate regions in the genome that potentially reflect local adaptations. They can also gauge whether populations are demographically “open” or “closed” (i.e., with global or local recruitment). We derived SNPs from double-digest restriction-site associated DNA (ddRAD) to test the demographics of commercially important bonefish Albula vulpes (N = 117) and queen conch Lobatus gigas (N = 60) from two northeast Caribbean Basin islands (Grand Bahama to the north and Eleuthera to the …

The Lost “Doe”: A Quality Improvement Project For Unidentified Patients, Brendi Gale

The Eleanor Mann School of Nursing DNP Capstone Projects

A Mass Casualty Incident (MCI) describes any natural or manmade disaster that stresses a community beyond their normal resources (CMS, 2019). Across the globe, populations have grappled with an increased frequency of natural disasters and a surge of critically ill secondary to pandemic SARs-CoV-2 (Cavallo, Donoho, & Forman, 2020; Smith, 2020; US Global Change Research Program, 2018). In addition, the incidence and severity of mass shootings has risen in the United States with a reported 277 active shooter events between the years of 2000-2018 (Federal Bureau of Investigation, 2018; Smith et al., 2019). An Emergency Department (ED) in Northwest Arkansas …

Effect Of Lactylate And Bacillus Subtilis On Growth Performance, Peripheral Blood Cell Profile, And Gut Microbiota Of Nursery Pigs, Xiaofan Wang, Tsungcheng Tsai, Xiaoyuan Wei, Bin Zuo, Ellen Davis, Tom Rehberger, Samantha Hernandez, Evelien J.M. Jochems, Charles Maxwell, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

To evaluate the effects of lactylate and Bacillus subtilis on growth performance, complete blood cell count, and microbial changes, 264 weaning pigs were assigned to four treatments (1) control (Con) basal diets that met the nutrient requirement for each phase, (2) 0.2% lactylate (LA), (3) 0.05% Bacillus subtilis strains mixtures (BM), or (4) the combination of LA and BM (LA+BM) added to the control basal diet at their respective inclusion rates in each of the three phases. Dietary lactylate tended to increase weight gain, significantly increased feed intake, and reduced fecal total E. coli and enterotoxigenic E. coli counts during …

Assessment Of Lippia Origanoides Essential Oils In A Salmonella Typhimurium, Eimeria Maxima, And Clostridium Perfringens Challenge Model To Induce Necrotic Enteritis In Broiler Chickens, Makenly E. Coles, Aaron J. Forga, Roberto Señas-Cuesta, Brittany D. Graham, Callie M. Selby, Alvaro J. Uribe, Blanca C. Martinez, Jaime A. Angel-Isaza, Christine N. Vuong, Xochitl Hernandez-Velasco, Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction …

Use Of Image Analysis To Identify Woody Breast Characteristics In 8-Week-Old Broiler Carcasses, Juan P. Caldas-Cueva, A. Mauromostakos, X. Sun, Casey M. Owens

Poultry Science Faculty Publications and Presentations

Woody breast (WB) condition causes significant economic losses to the global poultry industry, and the lack of an objective and fast tool to identify this myopathy is a contributing factor. The aim of this study was to determine if there are broiler carcass conformation changes that can be used to identify WB characteristics using image analysis. Images of 8-wk-old male broiler carcasses (n = 544) of high breast-yielding strains were captured before evisceration, which were processed and analyzed using ImageJ software. Measurements were as follows: M0, breast length; M1, breast width in the cranial region; M2, one-fifth of …

The Effect Of Feeding Adequate Or Deficient Vitamin B6 Or Folic Acid To Breeders On Methionine Metabolism In 18-Day-Old Chick Embryos, J. Lu, J. T. Weil, P. Maharjan, M. K. Manangi, S. Cerrate, C. N. Coon

Poultry Science Faculty Publications and Presentations

Three isotopic tracers ([2,3,3-²H₃]-L-serine, [²H₁₁]-L-betaine, and [1-¹³C]-L-methionine) were administered by amnion injection into 18-day-old chick embryos to investigate the kinetics of methionine metabolism. The embryos utilized were from eggs collected from 34-week-old Cobb 500 broiler breeders that were fed either a control diet containing folic acid (1.25 mg/kg diet) and pyridoxine HCl (5 mg/kg diet) or diets devoid of supplemental pyridoxine or folic acid. Intermediate metabolites of methionine metabolism and polyamines were analyzed in 18-day-old chick embryos. There were no differences in hepatic [²H₂] methionine or …

Research Note: Application Of An Escherichia Coli Spray Challenge Model For Neonatal Broiler Chickens, C. M. Selby, B. D. Graham, L. E. Graham, K. D. Teague, B. M. Hargis, G. Tellez-Isaias, C. N. Vuong

Poultry Science Faculty Publications and Presentations

Avian pathogenic Escherichia coli (E. coli) is an opportunistic pathogen often introduced to neonatal chicks during the hatching process. This commensal bacterium, particularly as a pioneer colonizer of the gastrointestinal tract, can have substantial implications in the rearing of poultry because of reduced flock performance. In order to mimic the effects of the natural bacterial bloom present during the hatch, a seeder challenge model was developed to expose neonatal chicks to virulent E. coli. On day 20 of embryogenesis, selected early hatched chicks (n = 18/hatcher) were briefly removed and sprayed challenged with saline (vehicle) or E. …

An Analysis Of The Patents Granted To The University Of Arkansas, Fayetteville, 2010-2019, Lutishoor Salisbury, Jeremy S. Smith, Tian Yang

University Libraries Faculty Publications and Presentations

This report provides an analysis of the patents were granted for ten years (2010-2019) where at least one of the inventors is affiliated with a department or college from the University of Arkansas in Fayetteville. It identifies the number of patents with inventors from the various departments; the number of unique inventors in each department; and the college affiliations of the inventors; their inter- and extra-collegiate collaborators from campus and their off-campus campus collaborators.

Sodium Butyrate Modulates Chicken Macrophage Proteins Essential For Salmonella Enteritidis Invasion, Anamika Gupta, Mohit Bansal, Rohana Liyanage, Abhinav Upadhyay, Narayan Rath, Annie Donoghue, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Salmonella Enteritidis is an intracellular foodborne pathogen that has developed multiple mechanisms to alter poultry intestinal physiology and infect the gut. Short chain fatty acid butyrate is derived from microbiota metabolic activities, and it maintains gut homeostasis. There is limited understanding on the interaction between S. Enteritidis infection, butyrate, and host intestinal response. To fill this knowledge gap, chicken macrophages (also known as HTC cells) were infected with S. Enteritidis, treated with sodium butyrate, and proteomic analysis was performed. A growth curve assay was conducted to determine sub-inhibitory concentration (SIC, concentration that do not affect bacterial growth compared …

Effects Of Dietary Fibers, Micronutrients, And Phytonutrients On Gut Microbiome: A Review, Kaleigh E. Beane, Mersady C. Redding, Xiaofan Wang, Jeong Hoon Pan, Brandy Le, Cara Cicalo, Suwon Jeon, Young Jun Kim, Jin Hyup Lee, Eui-Cheol Shin, Ying Li, Jiangchao Zhao, Jae Kyeom Kim

Animal Science Faculty Publications and Presentations

The human gastrointestinal tract harbors a magnitude of bacteria, which are collectively known as the gut microbiome. Research has demonstrated that the gut microbiome significantly impacts the health of its host and alters the host’s risk for various chronic diseases. Many factors, such as diet, could potentially be manipulated to alter the host gut microbiome and induce subsequent preventative and/or therapeutic effects. It has been established that diet partakes in the regulation and maintenance of the gut microbiome; however, specific crosstalk between the microbiome, gut, and host has not been clearly elucidated in relation to diet. In this review of …

Evaluation Of Three Formulations Of Essential Oils In Broiler Chickens Under Cyclic Heat Stress, Jared Ruff, Guillermo Tellez Jr., Aaron Forga, Roberto Señas-Cuesta, Christine N. Vuong, Elizabeth S. Greene, Xochitl Hernandez-Velasco, Alvaro J. Uribe, Blanca C. Martinez, Jaime A. Angel-Isaza, Sami Dridi, Clay J. Maynard, Casey M. Owens, Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The objective of the present research was to assess the dietary supplementation of three formulations of essential oils (EO) in chickens under heat stress (HS). Day-of-hatch Cobb 500 chicks (n = 500) were randomly distributed into four groups: 1. HS control + control diets; 2. HS + control diets supplemented with 37 ppm EO of Lippia origanoides (LO); 3. HS + control diets supplemented with 45 ppm LO + 45 ppm EO of Rosmarinus officinalis (RO) + 300 ppm red beetroot; 4. HS + 45 ppm LO + 45 ppm RO + 300 ppm natural betaine. Chickens that received …

Detection Of Woody Breast Condition In Commercial Broiler Carcasses Using Image Analysis, Juan P. Caldas-Cueva, A. Mauromostakos, X. Sun, Casey M. Owens

Poultry Science Faculty Publications and Presentations

Image analysis could be an objective and rapid method to identify woody breast (WB) myopathy and benefit the global poultry industry. The objective of this study was to determine if there are conformational changes that can be used to detect WB characteristics in commercial broiler carcasses across strains, gender, and ages using image analysis. A total of 900 images of male and female broiler carcasses from commercial standard and high breast-yielding strains and 5 ages (6 through 10 wk) were captured before evisceration. These images were processed and analyzed using ImageJ software. Conformational measurements were M0: breast length; …

Strategies For Change: Social Support, Erin Howie

Diet, Food, Exercise, and Nutrition (D-FEND)

What is Social Support?

Different types of social support.

Tips to improve our social network.

When Justice Should Precede Generosity: The Case Against Charitable Immunity In Arkansas, Courtney Jane Baltz

Arkansas Law Notes

This Comment discusses various aspects of the modern hospital and examines charitable immunity’s incompatibility with modern law.

First, Part II explains the historical justifications for immunity and presents the doctrine’s landscape in the United States. Part III examines the role precedent plays in continuing to adhere to the rule of immunity. Part IV takes an in-depth approach of the big business of hospitals by evaluating various financial aspects of charitable hospitals. Part V explores the reality of charitable immunity falling out of touch with concepts of modern law. Part VI takes a more specific look at the application of the …

Blood Driven Biopower Cells: Acquiring Energy From Reverse Electrodialysis Using Sodium Concentrations From The Flow Of Human Blood, Efecan Pakkaner, Chase Smith, Christina Trexler, Jamie Hestekin, Christa Hestekin

Chemical Engineering Faculty Publications and Presentations

With the growth of the healthcare industry, small scale power sources are needed to support advancements in medical implants, artificial organs, and sensors throughout the body. However, it remains a challenge to find safe, reliable, and stable power sources for these devices. In this study, a miniaturized reverse electrodialysis (RED) biopower cell design using physiological salinity concentration differences between renal bloodstreams is explored for its potential to be used as a power supply for implantable medical devices. Two small scale RED devices produced consistent power for up to three to ten days, depending on the device design. A two-fold decrease …

Conserved And Divergent Aspects Of Robo Receptor Signaling And Regulation Between Drosophila Robo1 And C. Elegans Sax-3, Trant Daiber, Christine J. Vanderzwan-Butler, Greg J. Bashaw, Timonthy A. Evans

Biological Sciences Faculty Publications and Presentations

The evolutionarily conserved Roundabout (Robo) family of axon guidance receptors control midline crossing of axons in response to the midline repellant ligand Slit in bilaterian animals including insects, nematodes, and vertebrates. Despite this strong evolutionary conservation, it is unclear whether the signaling mechanism(s) downstream of Robo receptors are similarly conserved. To directly compare midline repulsive signaling in Robo family members from different species, here we use a transgenic approach to express the Robo family receptor SAX-3 from the nematode Caenorhabditis elegans in neurons of the fruit fly, Drosophila melanogaster. We examine SAX-3’s ability to repel Drosophila axons from the …

Effects Of Dietary Energy Levels On Performance And Carcass Yield Of 2 Meat-Type Broiler Lines Housed In Hot And Cool Ambient Temperatures, Pramir Maharjan, Katie M. Hilton, Garrett Mullenix, Jordan Weil, Antonio Beitia, Nawin Suesuttajit, Cole Umberson, Diego A. Martinez, Justina Caldas, Antonio Kalinowski, Nadia Yacoubi, Victor Naranjo, Judith A. England, Craig N. Coon

Poultry Science Faculty Publications and Presentations

Two meat-type broiler lines, line A and line B were fed experimental diets from 22-42 d with objectives to determine the effects of dietary metabolizable energy (ME) levels on feed intake (FI), performance, body composition, and processing yield as affected by environmental grow-out temperatures. Two thousand fifty male chicks from line A and 2,050 male chicks from line B were reared in 90-floor pens, 45 chicks per pen utilizing primary breeder nutrition and husbandry guidelines for starter (110 d) and grower (11-21 d) phases. Experimental finisher diets consisted of 5 increasing levels of apparent nitrogen corrected …

Identification Of Snps Associated With Stress Response Traits Within High Stress And Low Stress Lines Of Japanese Quail, Steven Shumaker, Bhuwan Khatri, Stephanie Shouse, Dongwon Seo, Seong Kang, Wayne Kuenzel, Byungwhi C. Kong

Poultry Science Faculty Publications and Presentations

Mitigation of stress is of great importance in poultry production, as chronic stress can affect the efficiency of production traits. Selective breeding with a focus on stress responses can be used to combat the effects of stress. To better understand the genetic mechanisms driving differences in stress responses of a selectively bred population of Japanese quail, we performed genomic resequencing on 24 birds from High Stress (HS) and Low Stress (LS) lines of Japanese quail using Illumina HiSeq 2 × 150 bp paired end read technology in order to analyze Single Nucleotide Polymorphisms (SNPs) within the genome of each line. …

Research Note: Phytobiotics Modulate The Expression Profile Of Circulating Inflammasome And Cyto(Chemo)Kine In Whole Blood Of Broilers Exposed To Cyclic Heat Stress, Elizabeth S. Greene, Nima K. Emami, Sami Dridi

Poultry Science Faculty Publications and Presentations

Heat stress (HS) is a critical concern to the poultry industry as it affects both productivity and well-being. Various managerial and nutritional strategies have been proposed to mitigate the negative effects of HS in chickens, with plant-based additives showing promise. Recently, we reported the positive effect of a phytogenic feed additive (PFA) on growth performance in HS birds. Owing to the antioxidant nature of these compounds, we sought to further explore the effect of PFA on whole blood circulating chemokines, cytokines, and inflammasomes in HS broilers. Broilers (600 males, 1 d) were randomly assigned to 12 …

Development Of A Wild-Type Escherichia Coli Environmental Bloom Model To Evaluate Alternatives To Formaldehyde Fumigation In Broiler Chicken Hatch Cabinets, B. D. Graham, C. M. Selby, L. E. Graham, K. D. Teague, G. Tellez-Isaias, B. M. Hargis, C. N. Vuong

Poultry Science Faculty Publications and Presentations

Horizontal transmission of opportunistic Escherichia coli during hatch can have detrimental effects on early performance, particularly as pioneer colonizers. Commercially, formaldehyde is often applied in the United States to combat the bacterial bloom that occurs inside of the hatching environment. The purpose of these experiments was to develop a replicable E. coli horizontal challenge model to evaluate alternatives to formaldehyde sanitation applied to the hatching environment. In experiment 1, two trials were conducted for 2 wild-type (WT) E. coli isolates (isolate 1 [I1] or isolate 2 [I2]) to determine the appropriate in ovo challenge …

Migration Phenology And Patterns Of American Woodcock In Central North America Derived Using Satellite Telemetry, Joseph D. Moore, David E. Andersen, Tom Cooper, Jeffrey P. Duguay, Shaun L. Oldenburger, C. Al Stewart, David G. Krementz

Biological Sciences Faculty Publications and Presentations

American woodcock Scolopax minor (hereafter woodcock) migration ecology is poorly understood, but has implications for population ecology and management, especially related to harvest. To describe woodcock migration patterns and phenology, we captured and equipped 73 woodcock with satellite tracking devices in the Central Management Region (analogous to the Mississippi Flyway) of North America and documented migration paths of 60 individual woodcock and 87 autumn or spring woodcock migrations during 2014-2016. Woodcock migration at the scale of the Central Management Region was more synchronous in spring than in autumn, but unlike most other migratory birds, average duration of autumn migration (31 …

Soil Quality Indices As Affected By Long-Term Burning, Irrigation, Tillage, And Fertility Management, Helen C.S. Amorim, Amanda Ashworth, Kristofor R. Brye, Brian J. Wienhold, Mary C. Savin, Phillip R. Owens, Sergio Silva

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Understanding the impacts of long-term agricultural practices on soil quality (SQ) is key for sustaining agroecosystem productivity. This study investigated conventional and no-tillage (NT), residue burning and no burning, residue level (high and low), and irrigation (irrigated and dryland) effects on soil properties, SQ, and crop yields following 16 yr of a wheat (Triticum aestivum L.)–soybean [Glycine max (L.) Merr.] double-crop system via the Soil Management Assessment Framework (SMAF). A field experiment was conducted in the Lower Mississippi River Delta region on a silt-loam soil. Bulk density, soil organic C (SOC), total N (TN), pH, electrical conductivity (EC), …

Phytogenic Water Additives Improve Broiler Growth Performance Via Modulation Of Intermediary Metabolism-Related Signaling Pathways, Joshua J. Flees, Nima K. Emami, Elizabeth Greene, Bhaskar Ganguly, Sami Dridi

Poultry Science Faculty Publications and Presentations

A ban on the use of antibiotic growth promoters (AGPs) has fueled and promoted scientific research towards the identification of reliable and effective alternatives. The supplementation of phytogenics AV/SSL12 (AVSSL) and Superliv Gold (SG) in water has been shown to improve broiler feed efficiency (FE) via modulation of hypothalamic neuropeptides. However, their effects on peripheral metabolic pathways are still unknown. The present study was undertaken to determine the effects of AVSSL and SG on lipid and protein metabolism-associated pathways in various tissues. Day-old male Cobb 500 chicks (n = 288) were randomly assigned to 3 treatment groups, with 8 …

Phytogenic Feed Additives Improve Broiler Feed Efficiency Via Modulation Of Intermediary Lipid And Protein Metabolism-Related Signaling Pathways, Joshua J. Flees, Bhaskar Ganguly, Sami Dridi

Poultry Science Faculty Publications and Presentations

Diets enriched with phytogenic feed additives (PFA) such as AV/HGP/16 premix (AVHGP), Superliv concentrate premix (SCP), and bacteriostatic herbal growth promotor (BHGP) with essential oils have been shown to improve feed efficiency (FE) in broilers. This FE improvement was achieved via modulation of hypothalamic neuropeptides, which results despite feed intake reduction, in increased breast yield without changes in body weight compared to the control group. To gain further insights into the mode of action of these PFA, the present study aimed to determine the potential involvement of signaling pathways associated …

Using Verb Extension To Gauge Children’S Verb Meaning Construals: The Case Of Chinese, Weiyi Ma, Roberta Michnick Golinkoff, Lulu Song, Kathy Hirsh-Pasek

General Human Environmental Sciences Faculty Publications and Presentations

Verb extension is a crucial gauge of the acquisition of verb meaning. In English, studies suggest that young children show conservative extension. An important test of whether an early conservative extension is a general phenomenon or a function of the input language is made possible by Chinese, a language in which verbs are more frequent and acquired earlier. This study tested whether 3-year-old Chinese children extended a group of familiar verbs that specify various ways to carry objects. Shown videos that portrayed typical, mid-typical, or atypical carrying actions (as verified by Chinese adults), children were asked to judge whether they …

White-Matter Tract Connecting Anterior Insula To Nucleus Accumbens Predicts Greater Future Motivation In Adolescents, Josiah K. Leong, Tiffany C. Ho, Natalie L. Colich, Lucinda Sisk, Brian Knutson, Ian H. Gotlib

Psychological Science Faculty Publications and Presentations

The motivation to approach or avoid incentives can change during adolescence. Advances in neuroimaging allow researchers to characterize specific brain circuits that underlie these developmental changes. Whereas activity in the nucleus accumbens (NAcc) can predict approach toward incentive gain, activity in anterior insula (AIns) is associated with avoidance of incentive loss. Recent research characterized the structural white-matter tract connecting the two brain regions, but the tract has neither been characterized in adolescence nor linked to functional activity during incentive anticipation. In this study, we collected diffusion MRI and characterized the tract connecting the AIns to the NAcc for the first …

Structural Changes In The Oral Microbiome Of The Adolescent Patients With Moderate Or Severe Dental Fluorosis, Qian Wang, Xuelan Chen, Huan Hu, Xiaoyuan Wei, Xiaofan Wang, Zehui Peng, Rui Ma, Qian Zhao, Jiangchao Zhao, Jianguo Liu, Feilong Deng

Animal Science Faculty Publications and Presentations

Dental fluorosis is a very prevalent endemic disease. Although oral microbiome has been reported to correlate with different oral diseases, there appears to be an absence of research recognizing any relationship between the severity of dental fluorosis and the oral microbiome. To this end, we investigated the changes in oral microbial community structure and identified bacterial species associated with moderate and severe dental fluorosis. Salivary samples of 42 individuals, assigned into Healthy (N=9), Mild (N=14) and Moderate/Severe (M&S, N=19), were investigated using the V4 region of 16S rRNA gene. The oral microbial community structure based on Bray Curtis and Weighted …

Instrumental Texture Analysis Of Chicken Patties Prepared With Broiler Breast Fillets Exhibiting Woody Breast Characteristics, Juan P. Caldas-Cueva, A. Mauromostakos, Casey M. Owens

Poultry Science Faculty Publications and Presentations

Potential applications of chicken meat with the woody breast (WB) condition in further processed products could provide processors with alternatives to deal with this meat quality problem. The objective of this study was to evaluate the effect of the use of broiler breast fillets at varying degrees of WB severity and proportions on instrumental texture characteristics of chicken patties. A total of 54 breast fillets were collected from broilers processed as per commercial practices, previously classified based on tactile evaluation in 3 WB categories (normal [NOR]; mild [MIL], and severe [SEV]). Instrumental …