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Nutritional Ecology Of The Western Cottonmouth (Agkistrodon Piscivorus Leucostoma), Jason Ortega

Graduate Theses and Dissertations

Nutritional ecology aims to understand the factors that shape the diets of animals, how these ingested meals are processed, and how the assimilated nutrients are used to shape an organism’s interactions with its environment. As environmental parameters are altered due to anthropogenic changes, the availability and quality of prey may be altered. The alteration of the nutritional landscape can be devastating to nutritional specialists, yet generalist predators may be able to cope with these changes. The western cottonmouth (Agkistrodon piscivorus leucostoma) is a unique semiaquatic pitviper that can both forage along the land-water interface and in upland habitat when faced …

Effects Of Land Use On Soil Microbial Communities In Tropical Montane Forests Of Malaysian Borneo, Yang Kai Tang

Graduate Theses and Dissertations

Land use, such as logging and forest conversion to agriculture, can modify soil physicochemical and biological properties, and affect soil health. To understand how land use change can impact soil properties and canopy structure, we used a land use gradient in Malaysian Borneo consisting of six sites, including old growth forests, mixed forests, and agriculture fields. Specifically, we aimed to answer the following questions: (1) How do soil physicochemical properties vary across land use types? (2) Does bacterial diversity and composition vary across different land use types? (3) Does fungal diversity and composition vary across different land use types? We …

Approaches For Mitigating Woody Breast In Commercial Broilers Through Nutritional And Management Strategies, Clay Maynard

Graduate Theses and Dissertations

Breast myopathies have caused significant economic losses to the poultry industry over the last decade. The induction of woody breast (WB), white striping (WS), and spaghetti meat (SM) have caused textural issues, functional issues, and a decline in consumer acceptance. Of these three myopathies, WB causes the most pronounced effects on product quality and reduces functional use. Woody breast is also commonly associated with expansive breast meat growth/high performing broilers. However, progress in reducing these myopathies has been limited without hindering other aspects of poultry rearing. Generally, production performance reduces as myopathy occurrence or incidence reduces. This response is primarily …

Queer Need In The Natural State: An Exploratory Needs Assessment Of The 2slgbtqia+ Community In Northwest Arkansas, Leea Jean Johnson

Graduate Theses and Dissertations

This thesis explores the current needs of the 2sLGBTQIA+ community in Northwest Arkansas between the ages of 18-40 and what their experiences have been attempting to get their needs met. Eight semi-structured interviews were completed with members of the 2sLGBTQIA+ population, and ethical considerations were made throughout the process. The findings of this study were expansive and included six overarching themes with various subthemes. The findings report on the specific needs outlined by participants, how participants found the resources to meet their needs, and common positive and negative experiences that participants identified in the process. Findings showed that intersectional identities …

Influence Of Pregnant Cattle Grazing Endophyte-Infected Tall Fescue On Offspring Immune Function, Sidney Nicole Dunkel

Graduate Theses and Dissertations

The objective was to evaluate the influence of pregnant cattle grazing endophyte-infected tall, toxic, fescue (E+) during gestation on innate immune response of male offspring to an endotoxin challenge. Crossbred multiparous cows (n = 36) were bred to Red Angus sires and housed in replicated E+ or novel endophyte-infected, non-toxic, fescue (NE+) pastures prior to breeding and throughout gestation. From this calf-crop, a subset of post-weaned steers (n = 8 E+; n = 8 NE+) were selected for a lipopolysaccharide (LPS) challenge. Blood was collected every 30 min beginning 2 h prior to (Pre-LPS) and continuing 9 h following (Post-LPS) …

Molecular And Physiological Aspects Of Glufosinate Resistance In Amaranthus Palmeri, Matheus Machado Noguera

Graduate Theses and Dissertations

Palmer amaranth is one of the most troublesome weeds in worldwide agriculture. Among the traits that confers extreme weediness to this species, the ability to adapt to herbicide selection pressure stands out. The latest herbicide to which Palmer amaranth developed resistance is glufosinate-ammonium (GFA), an inhibitor of the enzyme glutamine synthetase (GS). The main objectives of this work were: 1) evaluate the resistance level of a Palmer amaranth population from Missouri, USA, 2) determine the mechanisms conferring GFA resistance in Palmer amaranth, 3) elucidate the behavior of amplified GS copies in GFA-resistant Palmer amaranth in terms of inheritance, stability and …

Cartographic Analysis Of Earth-Sun Relationships In Ancient Amazonia, Jackson Bennett Critser

Graduate Theses and Dissertations

The alignments of ancient man-made earthworks across the Amazon Basin, known as geoglyphs, have recently been discovered to predate early societal dates. Although much research indicated that the Amazon was uninhabitable until the last 1000 years (Meggers 1971), new evidence suggests this is not the case. The application of advanced cartographic and GIS technologies were implemented to link solar ‘marker’ days (e.g. solstices, equinoxes) with the alignment of geoglyphs, megaliths, stone architecture, and broader city forms to discover and analyze previously unknown Earth-Sun relationships across the Amazon Basin to conceivably sophisticated urban and architectural plans. The study of these geoglyphs …

An Ecological Perspective Of American Rodent-Borne Orthohantavirus Surveillance, Nathaniel Mull

Graduate Theses and Dissertations

Orthohantaviruses are a global group of viruses found primarily in rodents, though several viruses have also been found in shrews and moles. Many rodent-borne orthohantaviruses are capable of causing one of several diseases in humans, and the mortality associated with these diseases ranges from < 0.1% - 50% depending on the specific etiological virus. In North and South America, orthohantavirus research was ignited by an outbreak of severe disease in the Four Corners region of the United States in 1993. However, despite the discovery of over 20 orthohantaviruses in the Americas, our understanding of orthohantavirus ecology and virus-host dynamics in this region is still limited, and orthohantavirus surveillance is generally restricted in scope to select regions and small portions of host distributional ranges. In Chapter I, I present a literature review on the current understanding of American rodent-borne orthohantavirus ecology. This review focused on under-studied orthohantaviruses, addressing gaps in knowledge by extrapolating information from well-studied orthohantaviruses, general rodent ecology, and occassionally from Eurasian orthohantavirus-host ecology. There were several key conclusions generated from this review that warrant further research: 1) the large number of putative orthohantaviruses and gaps in orthohantavirus evolution necessitate further surveillance and characterization, 2) orthohantavirus traits differ and are more generalizable based on host taxonomy rather than geography, and 3) orthohantavirus host species are disproportionately found in grasslands and disturbed habitats. In Chapter II, I present a prioritized list of rodent species to target for orthohantavirus surveillance based on predictive modeling using machine learning. Probable orthohantavirus hosts were predicted based on traits of known orthohantavirus hosts using two different types of evidence: RT-PCR and virus isolation. Predicted host distributions were also mapped to identify geographic hotspots to spatially guide future surveillance efforts. In Chapter III, I present a framework for understanding and predicting orthohantavirus traits based on reservoir host phylogeny, as opposed to the traditional geographic dichotomy used to group orthohantaviruses. This framework establishes three distinct orthohantavirus groups: murid-borne orthohantaviruses, arvicoline-borne orthohantaviruses, and non-arvicoline cricetid-borne orthohantaviruses, which differ in several key traits, including the human disease they cause, transmission routes, and virus-host fidelity. In Chapter IV, I compare rodent communities and orthohantavirus prevalence among grassland management regimes. Sites that were periodically burned had high rodent diversity and a high proportion of grassland species. However, rodent seroprevalence for orthohantavirus was also highest in burned sites, representing a trade-off in habitat management outcomes. The high seroprevalence in burned sites is likely due to the robust populations supported by the high quality habitat resulting from prescribed burning. In Chapters V and VI, I describe Ozark virus and Sager Creek virus, two novel orthohantaviruses discovered from specimens collected during Chapter IV. Both chapters report full genome sequences of the respective viruses and compare both nucleotide and protein phylogenies with related orthohantaviruses. Additionally in Chapter VI, I support the genetic analyses with molecular and ecological characterizations, including seasonal fluctuations in host abundance, correlates of prevalence, evidence of virus shedding, and information on host cell susceptibility to Sager Creek virus.

Factors That Affect Home Range Of Timber Rattlesnakes (Crotalus Horridus) In Northwest Arkansas, Bannon Gallaher

Graduate Theses and Dissertations

Conservation of animal populations requires knowledge of their habitat and spatial needs. Quantifying spatial requirements involves the analysis of home range. We examined the effects of sex, body size (SVL), body condition (log mass/log SVL), and year on home range in Timber Rattlesnakes (Crotalus horridus) in Northwest Arkansas. Individual locality data from an ongoing, 22+ year radio-telemetry study in Madison Co., Arkansas were analyzed using both minimum convex polygon (MCP) and Kernel Density Estimates (KDE). Plots of the number of sequential observations versus home range (MCP and KDE) determined that a minimum of 25 locations per individual per active season …

Potential Control Of Invasive Mesquite Through Improving The Consumer’S Understanding Of Liquid Smoke Applications., Seth W. Ellsworth

Graduate Theses and Dissertations

Mesquite trees continue to invade forests and range lands in many countries across the world. The cost to remove these trees is staggering. In Texas, landowners spent $25 million over a 10- year period to clear 300,000 ha of mesquite trees, a fraction of the 22 million ha of Texas land affected by this invasion. Estimates are that the mesquite continues to negatively impact one to two percent of additional land in selected counties each year in Texas. However, the problem is not unique to Texas, but rather to the 44 species of mesquite trees, belonging to the genus Prosopis …

Effects Of Landscape And Yard Features On Mammals In Residential Yards In Northwest Arkansas, Emily Johansson

Graduate Theses and Dissertations

The human footprint is rapidly expanding, and wildlife habitat is continuously being converted to human residential properties. Most wildlife residing in developing areas are displaced to nearby undeveloped areas. However, some animals can coexist with humans and acquire the necessary resources (food, water, shelter) within the human environment. This may be particularly true when development is low intensity, as in suburban yards. Due to the wide variety in how homeowners utilize their yards, they can be considered individually managed “greenspaces.” These yards can provide a range of food (e.g., bird feeders, compost, gardens), water (bird baths and garden ponds), and …

Dietary Soy Galactooligosaccharides Affect The Performance, Intestinal Function, And Gut Microbiota Composition Of Growing Chicks, K. D. Teague, G. Tellez-Isaias, Jianmin Chai, V. Petrone-Garcia, C. N. Vuong, A. Blanch, S. H. Rasmussen, K. Brown, Jiangchao Zhao, S. J. Rochell

Poultry Science Faculty Publications and Presentations

The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were …

Effects Of Habitat Management On Rodent Diversity, Abundance, And Virus Infection Dynamics, Nathaniel Mull, Amy Schexnayder, Abigail Stolt, Tarja Sironen, Kristian M. Forbes

Biological Sciences Faculty Publications and Presentations

As anthropogenic factors continue to degrade natural areas, habitat management is needed to restore and maintain biodiversity. However, the impacts of different habitat management regimes on ecosystems have largely focused on vegetation analyses, with limited evaluation of downstream effects on wildlife. We compared the effects of grassland management regimes (prescribed burning, cutting/haying, or no active management) on rodent communities and the viruses they hosted. Rodents were trapped in 13 existing grassland sites in Northwest Arkansas, USA during 2020 and 2021. Rodent blood samples were screened for antibodies against three common rodent-borne virus groups: orthohantaviruses, arenaviruses, and orthopoxviruses. We captured 616 …

Transcriptome Profiling Of A Synergistic Volumetric Muscle Loss Repair Strategy, Kevin Roberts, John Taehwan Kim, Jacob Schluns, Grady Dunlap, Jamie Hestekin, Jeffery Wolchok

Biomedical Engineering Faculty Publications and Presentations

Volumetric muscle loss overwhelms skeletal muscle’s ordinarily capable regenerative machinery, resulting in severe functional deficits that have defied clinical repair strategies. In this manuscript we pair the early in vivo functional response induced by differing volumetric muscle loss tissue engineering repair strategies that are broadly representative of those explored by the field (scaffold alone, cells alone, or scaffold + cells) to the transcriptomic response induced by each intervention. We demonstrate that an implant strategy comprising allogeneic decellularized skeletal muscle scaffolds seeded with autologous minced muscle cellular paste (scaffold + cells) mediates a pattern of increased expression for several genes known …

Bioavailability Of Citrulline In Watermelon Flesh, Rind, And Skin Using A Human Intestinal Epithelial Caco-2 Cell Model, Inah Gu, Olugbenga Balogun, Cindi Brownmiller, Hye Won Kang, Sun-Ok Lee

Food Science Faculty Publications and Presentations

Watermelon produces many byproducts (watermelon rind and skin) even though those components contain various bioactive compounds, including citrulline. This study evaluated the citrulline concentration, total phenolic content, and antioxidant activity (DPPH and FRAP assays) of different parts of watermelon and investigated the bioavailability of citrulline from different parts of watermelon using an in vitro human intestinal epithelial Caco-2 cell monolayer model. Solid-phase extracted watermelon flesh, rind, and skin samples were treated on a Caco-2 cell monolayer for 1, 2, and 4 h. The collected basolateral solution at each time point was analyzed for the percentage of citrulline transport. Watermelon flesh …

Ins And Outs Of Rocker Switch Mechanism In Major Facilitator Superfamily Of Transporters, Stephanie Sauve, Joseph Williamson, Adithya Polasa, Mahmoud Moradi

Chemistry & Biochemistry Faculty Publications and Presentations

The major facilitator superfamily (MFS) of transporters consists of three classes of membrane transporters: symporters, uniporters, and antiporters. Despite such diverse functions, MFS transporters are believed to undergo similar conformational changes within their distinct transport cycles, known as the rocker-switch mechanism. While the similarities between conformational changes are noteworthy, the differences are also important since they could potentially explain the distinct functions of symporters, uniporters, and antiporters of the MFS superfamily. We reviewed a variety of experimental and computational structural data on a select number of antiporters, symporters, and uniporters from the MFS family to compare the similarities and differences …

Metagenomic And Meta-Transcriptomic Analysis Reveal The Colonization And Expression Profile Of Probiotic Strains In Humans And Animals, Yunjuan Peng, Routing Chen, Zhihao Zhang, Rui Jin, Ting Xe, Xinting Liu, Jianmin Chai, Samantha Howe, Jiangchao Zhao, Ying Li, Feilong Deng

Animal Science Faculty Publications and Presentations

In humans and animals, probiotics are widely accepted as crucial for host health and growth. The investigation of the probiotic colonization and expression of probiotics in the host is beneficial for proper usage of probiotics and isolation of indigenous probiotics. In this study, we analyzed commonly used probiotic strains in the intestines/rumen of humans and animals by analyzing metagenomic and paired meta-transcriptomic data from the gut or rumen microbiome of humans (n = 13), pigs (n = 6), chickens (n = 6), cattle (n = 14), sheep (n = 10), and mice (n = …

Comparative- And Network-Based Proteomic Analysis Of Bacterial Chondronecrosis With Osteomyelitis Lesions In Broiler's Proximal Tibiae Identifies New Molecular Signatures Of Lameness, Jennifer Cook, Elizabeth Greene, Alison Ramser, Garrett J. Mullenix, Jalila S. Dridi, Rohana Liyanage, Robert F. Wideman Jr., Sami Dridi

Poultry Science Faculty Publications and Presentations

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a specific cause of lameness in commercial fast-growing broiler (meat-type) chickens and represents significant economic, health, and wellbeing burdens. However, the molecular mechanisms underlying the pathogenesis remain poorly understood. This study represents the first comprehensive characterization of the proximal tibia proteome from healthy and BCO chickens. Among a total of 547 proteins identified, 222 were differentially expressed (DE) with 158 up- and 64 down-regulated proteins in tibia of BCO vs. normal chickens. Biological function analysis using Ingenuity Pathways showed that the DE proteins were associated with a variety of diseases including cell death, organismal …

The Role Classifiers Play In Selecting The Referent Of A Word, Weiyi Ma, Peng Zhou, Roberta Michnick Golinkoff

General Human Environmental Sciences Faculty Publications and Presentations

An important cue to the meaning of a new noun is its accompanying classifier. For example, in English, X in “a sheet of X” should refer to a broad, flat object. A classifier is required in Chinese to quantify nouns. Using children’s overt responses in an object/picture selection task, past research found reliable semantic knowledge of classifiers in Mandarin-reared children at around age three. However, it is unclear how children’s semantic knowledge differs across different types of classifiers and how this difference develops with age. Here we use an arguably more sensitive measure of children’s language knowledge (the intermodal preferential-looking …

Characterization Of Growth Patterns And Carcass Characteristics Of Male And Female Broilers From Four Commercial Strains Fed High Or Low Density Diets, C. J. Maynard, C. W. Maynard, A. R. Jackson, M. T. Kidd, S. J. Rochell, C. M. Owens

Poultry Science Faculty Publications and Presentations

Over the last few decades, the poultry industry has seen the emergence of various market segments that are beneficial for rearing various flock sizes. Two concurrent experiments consisting of 1,200 broilers each were conducted to evaluate the effects of broiler size and diet on the performance of four commercially available broiler strains, including 2 standard yielding (SY) and 2 high yielding (HY) strains. Within each experiment (Experiment 1: males, Experiment 2: females), a small bird (38 and 40 d processing) and big bird (47 and 54 d processing) debone market were targeted to give variable carcass …

Cholesterol In Class C Gpcrs: Role, Relevance, And Localization, Ugochi H. Isu, Shadi A. Badiee, Ehsaneh Khodadadi, Mahmoud Moradi

Chemistry & Biochemistry Faculty Publications and Presentations

G-protein coupled receptors (GPCRs), one of the largest superfamilies of cell-surface receptors, are heptahelical integral membrane proteins that play critical roles in virtually every organ system. G-protein-coupled receptors operate in membranes rich in cholesterol, with an imbalance in cholesterol level within the vicinity of GPCR transmembrane domains affecting the structure and/or function of many GPCRs, a phenomenon that has been linked to several diseases. These effects of cholesterol could result in indirect changes by altering the mechanical properties of the lipid environment or direct changes by binding to specific sites on the protein. There are a number of studies and …

Reducing Broiler Breast Myopathies Through Supplementation Of Guanidinoacetic Acid In Broiler Diets, C. J. Maynard, D. S. Nelson, S. J. Rochell, C. M. Owens

Poultry Science Faculty Publications and Presentations

Performance and processing advantages have been noted in meat animal production when excess arginine is available in the diet. In addition, dietary inclusion of guanidinoacetic acid (GAA) has provided insight on sparing arginine for broiler chickens. Therefore, a study was conducted to assess dietary inclusion of GAA on broiler breast myopathy prevalence. Dietary treatments were established using a control basal diet fed over four feeding phases and treatments were allocated as an additional supplementation of 0.06% (GAA 0.06) and 0.12% (GAA 0.12) in the form of CreAMINO (Alzchem Group, Trostberg, Germany). Bi-weekly BW, body weight gain (BWG …

Technology And Care To Promote Students' Success, Adnan Alrubaye

TFSC Publications and Presentations

Join the Wally Cordes Teaching and Faculty Support Center for an informal conversation about teaching with the February Cordes Chairperson, Dr. Adnan Alrubaye. Alrubaye teaches courses in both the poultry science and biological sciences departments, and he serves as the associate director for the cell and molecular biology graduate program. He teaches multiple sections of the General Microbiology course, with courses sizes ranging between 30 and 400 students. Alrubaye’s topic will be about tools he uses to provide the best learning experiences for students to help them achieve their learning goals. His philosophy when it comes to working with students …

Justice For Dogs, Alexander J. Lindvall

Arkansas Law Review

This Essay summarizes the Fourth Amendment’s protection of dogs. The Fourth Amendment protects people from unreasonable seizures. And nearly every circuit has held that it is unreasonable (and therefore unconstitutional) for an officer to shoot (seize) a dog without a very good reason. Killing a nonthreatening family pet is one of the most egregious forms of police misconduct. The courts rightfully recognize that the unjustified harming of a dog violates the Fourth Amendment.

Application Of Eugenol In Poultry To Control Salmonella Colonization And Spread, Mohammed Aljuwayd, Israa Abdullah Malli, Young Min Kwon

Poultry Science Faculty Publications and Presentations

The poultry sector is an essential component of agriculture that has experienced unprecedented growth during the last few decades. It is especially true for the United States, where the average intake of chicken meat increased from 10 pounds (4.5 kg) per person in 1940 to 65.2 pounds (29.6 kg) per person in 2018, while the country produced 113 billion eggs in 2019 alone. Besides providing nutrition and contributing significantly to the economy, chicken is also a natural reservoir of Salmonella, which is responsible for salmonellosis in humans, one of the significant foodborne illnesses around the globe. The increasing use of …

Constituent Loads And Trends In The Upper White River Basin: A Nonpoint Source Management Program Priority Watersheds, Erin M. Grantz, Brian E. Haggard

Arkansas Water Resources Center Technical Reports

The Arkansas Department of Agriculture – Natural Resources Division (ANRD) has identified the Upper White River Basin (UWRB; HUC 11010001) a hydrologic unit code (HUC) 8 watersheds, located in Northwest Arkansas, for prioritization by the Nonpoint Source (NPS) Management Program. UWRB includes Beaver Lake in its borders, the drinking water source for 1 in 6 Arkansans. Nonpoint source pollution concerns in these watersheds are excess nutrients from agriculture and sediment from changes in land use/land cover (LULC).

Local, state, and national groups, including the NPS Source Management Program, have invested in education, best management practices, and streambank restoration in the …

Constituent Loads And Trends In The Upper Illinois River Watershed: A Nonpoint Source Management Program Priority Watershed, Erin M. Grantz, Brian E. Haggard

Arkansas Water Resources Center Technical Reports

The Arkansas Department of Agriculture - Natural Resources Division (ANRD) has identified the Upper Illinois River Watershed (UIRW; 11110103), a hydrologic unit code (HUC) 8 watershed, located in Northwest Arkansas for prioritization by the Nonpoint Source (NPS) Management Program. URIW encompasses the Illinois River from its headwaters to the state line with Oklahoma and has been the subject of interstate disputes over water quality for decades. Nonpoint source pollution concerns in UIRW are excess nutrients from agriculture and sediment from changes in land use/land cover (LULC).

Local, state, and national groups, including the NPS Source Management Program, have invested in …

The Known And Unknowns Of Aphid Biotypes, And Their Role In Mediating Host Plant Defenses, Neetu Khanal, Christopher Vitek, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Insect species are subjected to disparate selection pressure due to various biotic and abiotic stresses. Management practices including the heavy use of chemical insecticides and introduction of insect-resistant plant cultivars have been found to accelerate these processes. Clearly, natural selection coupled with human intervention have led to insect adaptations that alter phenotypes and genetic structure over time, producing distinct individuals with specialized traits, within the populations, commonly defined as biotypes. Biotypes are commonly found to have better fitness in the new environment and, in the case of aphids, the most commonly studied system for biotypes, have the ability to successfully …

Local Il-10 Delivery Modulates The Immune Response And Enhances Repair Of Volumetric Muscle Loss Muscle Injury, Tai Huynh, Cassandra Reed, Zain Blackwell, Peyton Phelps, Luis Carlos Pinzon-Herrera, Jorge Almodovar, David A. Zaharoff, Jeffery Wolchok

Biomedical Engineering Faculty Publications and Presentations

This study was designed to test the hypothesis that in addition to repairing the architectural and cellular cues via regenerative medicine, the delivery of immune cues (immunotherapy) may be needed to enhance regeneration following volumetric muscle loss (VML) injury. We identified IL-10 signaling as a promising immunotherapeutic target. To explore the impact of targeting IL-10 signaling, tibialis anterior (TA) VML injuries were created and then treated in rats using autologous minced muscle (MM). Animals received either recombinant rat IL-10 or phosphate buffered saline (PBS) controls injections at the site of VML repair beginning 7 days post injury (DPI) and continuing …

A Molecular Signature Defining Exercise Adaptation With Ageing And In Vivo Partial Reprogramming In Skeletal Muscle, Ronald G. Jones Iii, Andrea Dimet-Wiley, Amin Haghani, Francielly Morena Da Silva, Camille R. Brightwell, Seongkyun Lim, Sabin Khadgi, Yuan Wen, Corey M. Dungan, Robert T. Brooke, Nicholas P. Greene, Charlotte A. Peterson, John J. Mccarthy, Steve Horvath, Stanley J. Watowich, Christopher S. Fry, Kevin A. Murach

Health, Human Performance and Recreation Faculty Publications and Presentations

Exercise promotes functional improvements in aged tissues, but the extent to which it simulates partial molecular reprogramming is unknown. Using transcriptome profiling from (1) a skeletal muscle-specific in vivo Oct3/4, Klf4, Sox2 and Myc (OKSM) reprogramming-factor expression murine model; (2) an in vivo inducible muscle-specific Myc induction murine model; (3) a translatable high-volume hypertrophic exercise training approach in aged mice; and (4) human exercise muscle biopsies, we collectively defined exercise-induced genes that are common to partial reprogramming. Late-life exercise training lowered murine DNA methylation age according to several contemporary muscle-specific clocks. A comparison of the murine soleus transcriptome …