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Lim Kinase 1 And Twinstar In Synaptic Plasticity Of Drosophila Motoneurons, James Brian Machamer Jan 2010

Lim Kinase 1 And Twinstar In Synaptic Plasticity Of Drosophila Motoneurons, James Brian Machamer

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Modification of the actin cytoskeleton is essential for synaptic plasticity, which is believed to underlie the brain's capacity for learning and memory. Regulation of the actin cytoskeleton by LIM Kinases (LIMK1 and LIMK2) and their downstream targets Actin Depolymerization Factor/Cofilin (ADF/Cofilin) in the mouse forebrain modulates dendritic spine morphology, synaptic function, long-term potentiation (LTP), and learning and memory, but appear dispensable for the regulation of most forms of presynaptic plasticity. However, these analyses were performed in mice mutant for single members of these gene families, and thus the interpretation of these results is confounded by potential compensatory activity of homologous …

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Translocation And Function Of Akt In The Mitochondria, Keri A. Mans Jan 2010

Translocation And Function Of Akt In The Mitochondria, Keri A. Mans

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The ubiquitously expressed kinase Akt is a known survival protein, and is involved in multiple cell signaling cascades, notably the phosphatidylinositol 3-kinase (PI3K) pathway. Active Akt translocates from the plasma membrane to different subcellular compartments, including mitochondria, where it phosphorylates compartment-specific substrates. The mechanism of this translocation and the specific function of Akt within the mitochondria remains a mystery. The goals of this study were to elucidate the mechanism by which Akt enters the mitochondria and examine the role of Akt in mitochondrial function. Finally, the possibilities of using post-mortem human brain tissue to study mitochondrial function in normal and …

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Translation Initiation Signaling Components Altered By Mechanical Load Dictate Skeletal Muscle Hypertrophy, David Lawrence Mayhew Jan 2010

Translation Initiation Signaling Components Altered By Mechanical Load Dictate Skeletal Muscle Hypertrophy, David Lawrence Mayhew

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The regulation of protein synthesis (i.e., mRNA translation) is an energetically costly and extensively regulated process, which is primarily regulated at the initiation step. Mechanical load is a potent stimulus of translation in skeletal muscle, and thus this tissue provides an excellent model system to study the plasticity of the translational apparatus. We investigated the effects of alterations in translation initiation cell signaling pathways in skeletal muscle using a variety of in vitro and in vivo mouse and human model systems. Collectively, our results suggest that, although many proteins in translational signaling pathways are responsive to mechanical load, the response …

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The Environmental And Linguistic Linkages Between Poverty And Education, Susan Mccarroll Jan 2010

The Environmental And Linguistic Linkages Between Poverty And Education, Susan Mccarroll

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Statistically, children who live in poverty are unfairly plagued primarily with a life of educational deficits. Researchers are constantly studying this phenomenon and have attributed several factors to the decrease in quality of life seen by impoverished children. For example, quality of the physical environment, of parental care, and of school systems all can play a large role in the intellectual ability of a child living in poverty. Moreover, children who have lower exposure to toxins, such as lead, have higher IQs and better reading ability than children who have had over-abundant exposure to negative environmental factors. Nationally, early education …

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A Comparative Study Of Radiological Threat Environments And Radiation Control, Lisa C. Mccormick Jan 2010

A Comparative Study Of Radiological Threat Environments And Radiation Control, Lisa C. Mccormick

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Radiation Control Programs (RCPs) differ from state to state as does their integration with public health agencies. The purpose of this study was threefold: to describe and compare across states the environmental radiological attributes of RCPs, to describe how these programs are organized structurally within state government, and to identify if RCPs differ structurally in environments of differing radiological attributes. Publicly available information from the Nuclear Regulatory Commission and state agency websites was used to determine both the environmental attributes and the different approaches states use to organize RCPs. States were grouped based on environmental attributes by employing a principle …

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What It Means For Grandparents To Raise Their Grandchildren As Primary Caregivers, Annette I. Mohan Jan 2010

What It Means For Grandparents To Raise Their Grandchildren As Primary Caregivers, Annette I. Mohan

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ABSTRACT For generations, grandparents have taken a special role in the lives of their grandchildren- to nurture, love, encourage and support them to achieve their goals. However, in the past fifteen years, the definition of family has become more flexible, as it accommodated the increasing number of grandparents raising grandchildren as primary caregivers. This research studies this family structure, which was seen as a solution to raise young children whose parents were unfit to perform their role as parents or because of a crisis. Grandparents who raise their grandchildren as primary caregivers were chosen using purposeful snowball sampling. The grandchildren …

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Genotyping Of Streptococcus Mutans Using Multilocus Sequence Typing, Stephanie Momeni Jan 2010

Genotyping Of Streptococcus Mutans Using Multilocus Sequence Typing, Stephanie Momeni

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ABSTRACT The role of Streptococcus mutans has been extensively studied using a variety of genotyping techniques. Repetitive extragenic palindromic polymerase chain reaction (rep-PCR) is an effective tool for screening large-scale epidemiological studies with high discriminatory power and reproducibility. For this study, multilocus sequence typing (MLST) analysis is used to evaluate genotypes previously identified as unique using rep-PCR. Twenty-two unique S. mutans rep- PCR genotypes were selected from a longitudinal study. Four additional isolates were selected from each genotype group of the 6 most commonly occurring genotypes (n=30) for further analysis. Real-time PCR was performed using eight housekeeping S. mutans gene …

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The Role Of Parental Autonomy Support In The Motivation Of The Health Behaviors Of Diet And Exercise In Older Adolescents, Shannon A. Morrison Jan 2010

The Role Of Parental Autonomy Support In The Motivation Of The Health Behaviors Of Diet And Exercise In Older Adolescents, Shannon A. Morrison

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regardless of the degree of heteroskedasticity. Moreover, in the presence of high leverage

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Effects Of Caregiving Strain On All-Cause Mortality, Martinique Perkins Jan 2010

Effects Of Caregiving Strain On All-Cause Mortality, Martinique Perkins

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Purpose. This study examined how perceived caregiving strain is related to quality of life, mental and emotional health, and mortality in a large, national sample. Methods. We used 3,714 caregivers from the REasons for Geographic and Racial Differences in Stroke (REGARDS) study. Participants had to complete baseline, in-home visit, and follow-up interviews to be included. Results. Approximately 12% of the REGARDS sample reported that they provided on-going care to a family member with chronic illness or a disability. Highly strained caregivers had the worst mental, physical, and emotional health but there was evidence of increased social support lessening the relationships …

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The Contribution Of Different Mechanisms Of Viral Sequence Variation To The Evolution Of Positive-Sense Single-Stranded Rna Viruses, Brett Earl Pickett Jan 2010

The Contribution Of Different Mechanisms Of Viral Sequence Variation To The Evolution Of Positive-Sense Single-Stranded Rna Viruses, Brett Earl Pickett

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The Flaviviridae family of positive-sense single-stranded RNA (+ssRNA) viruses includes viral taxa which greatly impact public health worldwide. To explore how the viruses within the Flaviviridae family evolve, we examined the extent to which these viral taxa use nucleotide covariance, spontaneous mutation, and/or homologous recombination to vary their genotype as well as the resulting phenotype. We developed and used CovarView to assist us in simultaneously viewing and inspecting the results from whole genome covariance analyses. This resulted in the identification of previously-characterized RNA functional structures in the genomes of hepatitis C virus (HCV), as well as a new RNA functional …

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Regulation Of Glycogen Synthase Kinase 3 In The Pathophysiology And Treatment Of Mood Disorders, Abigail Marie Polter Jan 2010

Regulation Of Glycogen Synthase Kinase 3 In The Pathophysiology And Treatment Of Mood Disorders, Abigail Marie Polter

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Mood disorders are devastating psychiatric illnesses that will affect as many as one in every five persons worldwide over the course of their lifetime. Significant gaps still remain in our understanding of these illnesses and outcomes for many patients are far from optimal. Glycogen Synthase Kinase 3 (GSK3) is a protein kinase that is increasingly recognized as playing an important role in a number of neuronal functions, as well as in pathological states such as mood disorders. GSK3 is inhibited in the brain by phosphorylation on an N-terminal serine. Several treatments for mood disorders, including lithium, antidepressants, and atypical antipsychotics …

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Assessing Knowledge, Attitudes, And Beliefs Of Teachers In Alabama Concerning Comprehensive Hiv Education, Steven Brent Powell Jan 2010

Assessing Knowledge, Attitudes, And Beliefs Of Teachers In Alabama Concerning Comprehensive Hiv Education, Steven Brent Powell

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The Alabama State Department of Education has guidelines for educators to insure HIV information is taught by teachers starting in fifth grade (Alabama Course of Study: Health Education, 2009). As assessed by The School Health Profile (Profiles), 55% of Alabama teachers received professional development on HIV/AIDS. In addition, less than half of grades 9-12 teachers taught how condoms work, the importance of consistent condom use, and how to obtain condoms (U.S. Department of Health and Human Services, 2009). The Youth Risk Behavior Surveillance Survey (YRBSS) administered to grades 9-12 determined that 57% of youth in Alabama are sexually active, with …

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Cognitive-Behavioral Intervention To Enhance Communication Skills For Cancer Patients, Heather Prayor-Patterson Jan 2010

Cognitive-Behavioral Intervention To Enhance Communication Skills For Cancer Patients, Heather Prayor-Patterson

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Introduction: With nearly 12 million Americans living with or having a history of cancer, the patient-doctor communication process is especially important for this population. Cancer patients are often dissatisfied with the quality of the information they receive regarding their treatment, side effects, symptom control, and the quality of the relationship with their healthcare provider. A patient's self-efficacy during patient-doctor interactions influences the level of trust in his/her doctor, satisfaction with care, and the type of information exchanged. Studies have focused on intervening in patient communication, but there are limitations in the literature, including a focus on improving self-efficacy, addressing communication …

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Exploring The Effects Of Limited English Language Proficiency On Disability: A Pilot Study Of Adult Chinese-, Japanese-, And Korean-Language Speakers In The United States, Xiaofei Qiao Jan 2010

Exploring The Effects Of Limited English Language Proficiency On Disability: A Pilot Study Of Adult Chinese-, Japanese-, And Korean-Language Speakers In The United States, Xiaofei Qiao

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This is a pilot study contending that limited language proficiency is associated with disability outcomes for Chinese-, Japanese-, and Korean-speaking Americans in the United States, as determined by application of the Disablement Process model. Using the 5 percent public-use micro-data samples (PUMS) from the United States Census 2000, this study explores the relationship by employing a Poisson regression on these three language groups from a comparative perspective. The findings shed light on the significant effects of limited language proficiency as a predisposing risk factor on disability outcomes and also demonstrate the importance of individual, family, and community characteristics as either …

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Response Dynamics Of Retinal Ganglion And Early Visual Cortical Cells To Simulated Optical Blur, Michael Lance Risner Jan 2010

Response Dynamics Of Retinal Ganglion And Early Visual Cortical Cells To Simulated Optical Blur, Michael Lance Risner

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Visual neurons are highly sensitive to changes in luminance around the mean level of light. The effects of changes in luminance contrast on the responses of retinal ganglion cells (RGCs) and V1 neurons have been extensively studied. Sharp transitions in luminance signify edges, which are key features in the visual environment. However, edges are rarely sharp and may be blurred. Edges are generally blurred because eyes have a restricted depth of field, sources of light are rarely point sources, edges are typically smooth, and rapid motion induces blur. The purpose of this research was to understand how RGCs and V1 …

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Heparanase Drives The Aggressive Myeloma Phenotype: Preclinical Development Of A Heparanase Inhibitor For The Treatment Of Multiple Myeloma, Joe Ritchie Jan 2010

Heparanase Drives The Aggressive Myeloma Phenotype: Preclinical Development Of A Heparanase Inhibitor For The Treatment Of Multiple Myeloma, Joe Ritchie

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Heparanase, an endoglycosidase which cleaves heparan sulfate chains at specific sites, is rarely expressed in normal tissues but becomes evident in many human cancers. We have previously shown that heparanase promotes myeloma growth and angiogenesis through modulation of the tumor microenvironment. Recognition that heparanase drives the aggressive myeloma phenotype has led to new strategies designed to therapeutically target this enzyme. SST0001, a non-anticoagulant heparin that is 100% N-acetylated and 25% glycol split, was previously described as a potent inhibitor of heparanase activity in vitro, and, in limited in vivo experiments, SST0001 was identified to have efficacy as an anti-tumor agent …

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Principals Mentoring Teachers To Improve Instructional Practices: A Multiple Case Study, Walter Burke Wren Jan 2010

Principals Mentoring Teachers To Improve Instructional Practices: A Multiple Case Study, Walter Burke Wren

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ABSTRACT The purpose of this multiple case study was to examine the mentoring relationship between principals and their teachers in regards to instructional practice at three demo-graphically diverse elementary schools. Multiple data sources were used to conduct qua-litative research which resulted in extensive data collection and analysis. The collection of data was conducted through direct observations, semi-structured interviews, and a review of documents provided by each research site. This case study used the theories of adult learning, instructional leadership and transformational leadership to explain the findings, provide focused implications for mentoring processes between principals and teachers, and to influence the …

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Therapeutic Interventions For Chlorine Gas Induced Lung Injury, Amit Kumar Yadav Jan 2010

Therapeutic Interventions For Chlorine Gas Induced Lung Injury, Amit Kumar Yadav

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Rationale: Nitrite (NO2-) has been shown to limit injury to the heart, liver and kidneys in various models of ischemia-reperfusion injury. Currently, potential protective effects of systemic NO2- in limiting lung injury or enhancing repair have not been documented. Objectives: To assess the efficacy and mechanisms by which post-exposure intra-peritoneal injections of NO2- mitigate Cl2 induced lung injury in rats. Methods: Rats were exposed to Cl2 (400 ppm) for 30 minutes and returned to room air. Nitrite (1mg/Kg) or saline were administered intraperitoneally at 10 min, 2, 4 and 6 hrs post exposure. Rats were sacrificed at 6 hrs or …

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The Role Of Map Kinase Cascade In Msp Signaling Response, Youfeng Yang Jan 2010

The Role Of Map Kinase Cascade In Msp Signaling Response, Youfeng Yang

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The MSP domain is an evolutionarily conserved immunoglobulin-like structure of about 120 amino acids (Miller et al., 2001). A P56S missense mutation in the MSP domain of the human vapb gene is associated with a dominantly inherited form of Amyotropic Lateral Sclerosis (ALS) or Spinal Muscular Atrophy (SMA) (Nishimura et al., 2004). In C. elegans, secreted MSP binds to the VAB-1 Eph receptor and other receptors on oocyte and ovarian sheath cell surfaces (Miller et al., 2003). We have recently shown that the P56S mutation prevents secretion of the VAPB MSP domain, suggesting that the signaling function is important in …

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Il-21 Dictates The Quality Of The Cd8 T Cell Response During Acute And Chronic Infections, John Seung-Hoon Yi Jan 2010

Il-21 Dictates The Quality Of The Cd8 T Cell Response During Acute And Chronic Infections, John Seung-Hoon Yi

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CD8 T cells contribute to the control of intracellular pathogens including viruses and certain bacteria. Recognition of foreign antigens by antigen-specific CD8 T cells initiates a differentiation process by which CD8 T cells gain effector functions including the ability to rapidly produce cytokines and kill infected cells. Currently, it is not well understood what signals dictate the generation of robust CD8 T cell responses, however it has been demonstrated that without CD4 T cell help CD8 T cell responses are compromised. CD4 T cells are the primary producers of interleukin-21 (IL-21), which has broad immunological actions. Thus for this dissertation, …

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Cardiovascular Endothelial Regeneration On An Electrospun Scaffold, Xing Zhang Jan 2010

Cardiovascular Endothelial Regeneration On An Electrospun Scaffold, Xing Zhang

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A bi-layer electrospun nano-fibrous scaffold comprising synthetic polymers and natural proteins was constructed as the prototype of an arterial substitute for coronary ar-tery disease. This scaffold featured a highly porous microstructure analogous to native extracellular matrix. Uni-axial tensile test suggested that its excellent mechanical strength could restore normal blood flow immediately after implantation. Subsequent biocompati-bility study demonstrated that scaffolding materials, either traditional or degraded, were non-toxic to the regenerated human endothelium on the luminal layer. Moreover, this re-generated endothelium possessed critical functions, such as the formation of tight junc-tions, the secretion of prostacyclin, and the prevention of platelet adhesion. Thereafter, …

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A Ck2-Dependent Mechanism For Activation Of The Jak-Stat Signaling Pathway: Implications For Cancer Biology, Ying Zheng Jan 2010

A Ck2-Dependent Mechanism For Activation Of The Jak-Stat Signaling Pathway: Implications For Cancer Biology, Ying Zheng

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Janus Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) signaling is involved in regulation of cell survival, proliferation and differentiation. JAK tyrosine kinases can be transiently activated by cytokines or growth factors in normal cells, while they become constitutively activated as a result of mutations that affect their function in tumors. Specifically, the JAK2V617F mutation is present in the majority of patients with myeloproliferative disorders (MPDs) and is implicated in the pathogenesis of these diseases. In this dissertation, we report the identification of the serine/threonine kinase CK2 as a novel interactor and important regulator of activation of the JAK-STAT signaling …

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The Influence Of Self-Concept, Physical Activity, And Sedentary Screen Time Activities On Blood Pressure In 11-13 Year Old Middle School Children, Elizabeth Di Benedetto Gulledge Jan 2010

The Influence Of Self-Concept, Physical Activity, And Sedentary Screen Time Activities On Blood Pressure In 11-13 Year Old Middle School Children, Elizabeth Di Benedetto Gulledge

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The aims of this study were to: 1) examine associations between self-concept (SC), physical activity (PA), sedentary screen time (SST), and blood pressure (BP) and 2) determine the amount of variance explained by SC, PA, and SST in BP. The increase in SST along with decreases in PA may contribute to increases in BP. SC, PA and SST have been associated with BP in adolescents but less is known about middle school children. A sample of 116 (36 males; 80 females; 51 African American, 65 White) 6th and 7th grade11-13 year old students were recruited in a southeastern state. Participants …

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Information Technology And Quality Of Care In Pediatric Hospitals, Gouri Gupte Jan 2010

Information Technology And Quality Of Care In Pediatric Hospitals, Gouri Gupte

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The purpose of this dissertation is to examine the relationship between the adoption of information technology by pediatric hospitals and performance on several quality of care measures. This research is aimed at addressing a gap in literature by utilizing a relatively large sample size that examines health information technology adoption across institutions and utilizes validated quality indicators from Agency for Healthcare Research and Quality. The Donabedian evaluation model that encompasses "structure, process, and outcomes" provides the conceptual framework for the organization of this research, selection of variables, and analysis. Three secondary datasets were used, 2005 Information Technology Survey of the …

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A Pilot Study For A Computer-Tailored Program To Increase Calcium And Vitamin D Consumption In Young Women: Does Adding Quantitative Ultrasound Change Outcomes?, Donald Henry Lein Jan 2010

A Pilot Study For A Computer-Tailored Program To Increase Calcium And Vitamin D Consumption In Young Women: Does Adding Quantitative Ultrasound Change Outcomes?, Donald Henry Lein

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Osteoporosis and osteoporosis-related fractures are costly personal and national problems. No cure exists so experts stress early practice of bone protective behaviors, like adequate calcium and vitamin D intake, to prevent osteoporosis and osteoporosis-related fractures. However, young women in the United States do not consume adequate amounts of these two nutrients. Further evaluation to identify cost effective programs to increase calcium and vitamin D consumption in young women is needed. The purpose of this study was to evaluate whether quantitative ultrasound (QUS) with bone strength feedback to a computer-tailored feedback and counseling intervention was needed to increase calcium and vitamin …

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Effect Of Overexpressing Apolipoprotein A-I In An Animal Model Of Alzheimer's Disease, Terry L. Lewis Jan 2010

Effect Of Overexpressing Apolipoprotein A-I In An Animal Model Of Alzheimer's Disease, Terry L. Lewis

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Apolipoprotein A-I (apoA-I) is the major protein component of high density lipoprotein (HDL) in circulation and is expressed mainly by the liver and intestine. The levels of apoA-I/HDL are inversely related to the incidence of cardiovascular disease. Because of the connections between heart disease and Alzheimer's disease (AD), it is conceivable that high levels of apoA-I/HDL may be protective against AD. However, the limited literature shows mixed results on the role of apoA-I/HDL in the development of AD. It is hypothesized that increased expression of human apoA-I will ameliorate the behavioral deficits and characteristic amyloid-ß (Aß) plaque formation in a …

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Protective Antibodies To The Proline-Rich And Alpha Helical Regions Of Pneumococcal Surface Protein A And Their Detection In An In Vitro Surrogate Assay, Calvin Clay Daniels Jan 2010

Protective Antibodies To The Proline-Rich And Alpha Helical Regions Of Pneumococcal Surface Protein A And Their Detection In An In Vitro Surrogate Assay, Calvin Clay Daniels

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Current pneumococcal vaccines containing pneumococcal capsular polysaccharide antigens protect against infection by pneumococci. However, this protection is limited to strains expressing the capsule types included in the vaccine. Since the introduction of these vaccines, the increase in incidence of infection by serotypes not targeted by the vaccine, a process called "serotype replacement," and the growing antibiotic resistance of replacement strains both stress the need for vaccines with broader protection. The alpha-helical region of a protein present on all clinical isolates of pneumococci, Pneumococcal Surface Protein A (PspA), elicits protective immunity. Downstream of the alpha-helical region is a highly conserved region …

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Characterization Of Explanted Endovascular Stents: A Study Of In-Vivo Corrosion And The Role Of Vascular Calcification In Stent Fracture, Dina Halwani Jan 2010

Characterization Of Explanted Endovascular Stents: A Study Of In-Vivo Corrosion And The Role Of Vascular Calcification In Stent Fracture, Dina Halwani

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The electrochemical and mechanical stability of endovascular stents in-vivo is essential for their biocompatibility. Limited information is available regarding the condition of stents following interaction with vascular tissue, as a result, twenty seven stented vessels containing 45 stents (33 bare metal and 12 drug eluting) were retrieved during autopsy and vascular surgery. The stent/tissue components were carefully separated using a rigorous tissue dissolution protocol. Stent surfaces were characterized using 3D digital optical and scanning electron microscopy. Transferred metallic elements in dissolved tissue were measured using mass spectroscopy. Histopathologic assessment of stented tissue, when available, was also performed. Fifteen out of …

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Fertility Enhancing Drugs, Assisted Reproductive Technologies And Effects On Maternal And Infant Outcomes Among Live Births In Texas, Suzanne Jay Roseman Jan 2010

Fertility Enhancing Drugs, Assisted Reproductive Technologies And Effects On Maternal And Infant Outcomes Among Live Births In Texas, Suzanne Jay Roseman

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Most studies evaluating outcomes among infertility treatment users use a spontaneously conceived (SC) group for comparative purposes, despite obvious limitations to this approach. Further, lack of comprehensive U.S. data-sources recording infertility treatments, especially non-assisted reproductive technology (non-ART) infertility treatments, has hampered the ability of U.S. researchers to explore the effects of a particular infertility treatment modality (for example ART) on maternal and infant outcomes while controlling for the effects of infertility itself. The present study makes use of the new data item: "Pregnancy resulted from infertility treatment," further categorized into ART and non-ART infertility treatments--from the 2003 revision of birth …

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Drawing A Bath: Works By Robert Henri And George Bellows At The Birmingham Museum Of Art, Mary K. Russell Jan 2010

Drawing A Bath: Works By Robert Henri And George Bellows At The Birmingham Museum Of Art, Mary K. Russell

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This thesis discussing bathing images by Robert Henri and George Bellows located at the Birmingham Museum of Art.

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