Digital Commons Network^™

Protection Against Neutrophil Extracellular Trap (Net) Toxicity By Antioxidant Monoher, Puji Astuti, Danielle M A Beurskens, Tanja Vajen, Gerry A F Nicolaes, Ming Zhang, Guido R M M Haenen

Makara Journal of Health Research

Background: Neutrophil extracellular traps (NET) are extracellular fibers produced by activated neutrophils to kill bacteria. NET was recently found to be associated with several diseases, such as autoimmune diseases. NET formation, called NETosis, is reactive oxygen species (ROS) dependent, thereby, prompting us to study its inhibition by potent antioxidant monoHER as well as to study monoHER protection against NET toxicity caused by NET constituent histone 3 on endothelial cells. Methods: Freshly isolated neutrophils from male donors were stimulated with PMA to induce NET formation. The effect of monoHER (50 µM) on oxidative burst (O2●− production) and NET formation was determined …

The Effectiveness Of Geographic Information System Training For Surveillance Officers In Sukabumi District, Martya Makful

ASEAN Journal of Community Engagement

Surveillance is the systematic, continuous collection, processing, analysis and interpretation of data and the dissemination of information to units in need to be able to take action. In the field of health, human resources (HR) in an organization is very important role. Because the quality of health services to the public is determined by the human resources working in it. With the number of reports and cases of outbreaks in Sukabumi district, it is necessary a system of information and recording of data that muktahir. The purpose of this research is to know the effectiveness of training of geographic information …

Implementation Of Control Policy Of Diphtheria Outbreak And Outbreak Response Immunization (Ori) In Kota Depok, 2017, Dian Novita Sari, Anhari Achadi

Journal of Indonesian Health Policy and Administration

Diphtheria is a contributor to an outbreak (KLB) for some regions in Indonesia, included West Java Province. Diphtheria cases had increased through 2015 - 2016, the cases increased from 59 cases to 153 cases. Depok City became one of the contributors of diphtheria cases that have fluctuating incidents. Disease trends have decreased in 2013-2015 but then increased in 2016 to 8 cases. This study discusses policy implementation, viewed from policy sources, resource arrangements, the characteristics of implementing agencies, bureaucratic structures, communications, the influence of dispositions and socio-economic and political circumstances in control of Diphtheria Outbreak and Outbreak Response Immunization (ORI) …

Celac: Mexican Foreign Policy Interest On Latin American And Caribbean Regionalism, Demeiati Nur Kusumaningrum

Global: Jurnal Politik Internasional

La Comunidad de Estados Latinoamericanos y Caribeños (CELAC) was initiated in 2011 as a forum for dialogue and consensus between Latin American and Caribbean governments where it forms a trading block that excludes the United States and Canada. The US objected to the establishment of CELAC which seemed to challenges the former regional organization (OAS). This paper aims to analyze the considerations of the Mexican government to join CELAC in the midst of mutual agreement with US and NAFTA. Theory of formulation of foreign policy is used to answer the sources of political consideration of the Mexican government. It is …

Association Between Silica Dust Exposure And Serum Transforming Growth Factor-Β1 In Stone Quarry Workers, I Putu Eka Krisnha Wijaya, Ida Bagus Ngurah Rai, I Putu Andrika

Jurnal Penyakit Dalam Indonesia

Introduction. Silicosis is a disease that most often occurred as a result of silica dust exposure. Silica dust particles will stimulate alveolar macrophages to secrete proinflammatory mediators for fibroblast proliferation and collagen deposition. TGF-ß1 is one of the most extensively involved mediators in the development of fibrosis. To date, several studies have not shown consistent results regarding the association between silica dust exposure and TGF-β1. This study examined silica dust exposure and serum TGF-β1, which aims to determine the risk of silicosis in stone quarry workers. Methods. An analytic cross-sectional study was conducted at Company X, Karangasem in March 2016. …

Correlation Between Proper Antibiotics Administration To Community Acquired Pneumonia Patients Based On Alur Gyssens With Clinical Response, Cleopas Martin Rumende, Lie Khie Chen, Anis Karuniawati, Joyce Bratanata, Rezyta Falasiva, Truly Panca Sitorus, Erwin Christian Susanto

Jurnal Penyakit Dalam Indonesia

Introduction. The proper of antibiotics treatment will increase the improvement of the patient with community-acquired pneumonia (CAP) and also counteract antimicrobial resistance. Community-Acquired Pneumonia is commonly found in Indonesia with an incidence of 2.4%. This study aimed to determine the proper of antibiotic selection for CAP based on IDSA-ATS 2007 guidelines with Gyssens method and evaluate the relationship between the proper of antibiotic treatment with the clinical response of patients. Methods. A retrospective cohort study was conducted with secondary data from the previous study. Study was conducted in three hospitals in Jakarta, namely Cipto Mangunkusumo General Hospital, Budhi Asih Hospital, …

Karakteristik Pasien Tuberkulosis Lost To Follow Up Dari Empat Rs Di Kota Bandung, Azizah Muthiah, Noormarina Indraswari, Budi Sujatmiko

Jurnal Epidemiologi Kesehatan Indonesia

Jumlah kasus tuberkulosis (TB) yang tidak terlaporkan dan tidak terdeteksi di Indonesia sebelum pelaksanaan sistem pengawasan TB nasional pada tahun 2017 mencapai 47% dari total kasus, termasuk di dalamnya kasus TBlost to follow up atau hilang dari pengamatan. Pasien yang termasuk dalam kelompok ini akan meningkatkan risiko perburukan klinis, kambuh, gagal pengobatan, dan menjadi resistan terhadap obat, selain itu mereka juga akan mejadi sumber penularan di masyarakat. Tujuan penelitian ini yaitu untuk mengetahui karakteristik pasien rawat jalan TB yang hilang dari pengamatan dari empat rumah sakit terpilih di Kota Bandung. Penelitian ini menggunakan studi deskriptif dengan rancangan potong lintang. Sampel …

Pengaruh Penerapan Healthcare Infection Control Practices Advisory Committee Terhadap Kejadian Infeksi Saluran Kemih Terkait Penggunaan Kateter, Wendy Isman Sitepu

Jurnal ARSI : Administrasi Rumah Sakit Indonesia

An estimated 0,2-4,8 Catheter-associated Urinary Tract Infection (CAUTI) cases 1.000 catheter-days affected around the world. CAUTI not only has affected the patient but also has became a burden on hospital. Many prevented CAUTI guidelines has developed, one of them is issued by Healthcare Infection Control Practices Advisory Committe (HICPAC). The study aimed to evaluate HICPAC guideline decreasing CAUTI rate. A quasi-experimental study was performed to ass’s effectiveness HICPAC guidelines with two group pretest-posttest approach patients with catheter 150 samples each. Before implementation, all medic staff was explained and trained about the guideline. All medical staff was assessed their knowledge, attitudes, …

Recurrent Perianal Wart Therapy With 90% Trichloroacetate (Tca) Solution In Aids Patients, Sri Linuwih Sw Menaldi, Ika Anggraini, Hanny Nilasari

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Being the most commonly found sexually-transmitted infection (STI), anogenital warts are mainly caused by human papilloma virus (HPV) type 6 and 11. These infections are often recurrent, leading to loss of time and medical expenses. Various therapeutic modalities can be chosen singularly or in combination with varying results. Treatments with less responsive results usually occur when acquired comorbidities are present, such as HIV infection.

Case Illustration: A 28-year-old homosexual male was reported to have a recurrent perianal wart for the duration of eight months. He was also diagnosed with AIDS and pulmonary tuberculosis. Initially, patient was treated …

Dapsone Hypersensitivity Syndrome Overlaps Reversal Reaction In Leprosy Patient, Chesia Christiani Liuwan, Medhi Denisa, M Yulianto Listiawan

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Leprosy is a chronic granulomatous infectious disease that primarily involves the skin and peripheral nerve and is caused by Mycobacterium leprae. Clinical features may vary depending on the patient’s immune response. Reversal reaction due to increased immunity is common after treatment in patients with borderline leprosy. Dapsone hypersensitivity syndrome (DHS) is an adverse effect from dapsone therapy that may occur in 5-6 weeks to 6 months after initial treatment.

Case Illustration: A 47-year-old female complained of a spreading red patches on the skin of her trunk and extremities for three weeks duration. She also felt itchiness and …

Gallstone Ileus In Cipto Mangunkusumo General Hospital, Jakarta: A Case Series, Toar Jm Lalisang, Georgina P. Hehuwat, Arnetta Nl Lalisang, Irfan K. Pratama, Yarman Mazni

The New Ropanasuri Journal of Surgery

Introduction. Gallstone ileus is an uncommon mechanical bowel obstruction caused by a gallstone in the gastrointestinal tract which enters due to bile-enteric fistulae. This study aims to describe gallstone ileus and its management. Method. Data were retrospectively collected from medical records. Clinical manifestations, laboratory data, supporting radiographic examinations, treatment, postoperative care, and outcomes were collected. Results. We report two gallstone ileus cases at Cipto Mangunkusumo General Hospital, Jakarta which admitted in the last 20 years. The first case was a woman 33 years in 2002 and the second was man 45 years in 2017. Ileus was the main clinical symptoms. …

Sequence Variability In The Nramp1 Geneamong Indigenous And Exotic Chicken Types, Abazuh Uchenna Desmond, Adekoya Khalid Olajide, Oboh Bola Olufunmilayo

Makara Journal of Science

The natural resistance-associated macrophage protein 1 (NRAMP1) gene in chickens, which exists on chromosome 7, is thought to play a significant role in disease resistance. Variations in this gene have been documented and have played crucial roles in the variations in the resistance and/or susceptibility that is expressed by individuals and different groups of animal species. In this study, the NRAMP1 gene was studied for single nucleotide polymorphism (SNP) variability between indigenous and exotic chicken breeds. The gene was amplified using the polymerase chain reaction (PCR) method and sequenced and analyzed. Six SNPs, both synonymous and non-synonymous (C3700T, G3702C, A3712C, …

Dengue Haemorrhagic Fever And House Conditions In Kupang City, East Nusa Tenggara Province, Wanti Wanti, Ririh Yudhastuti, Hari Basuki Notobroto, Sri Subekti, Oktofianus Sila, Ragu Harming Kristina, Feby Dwirahmadi

Kesmas

Kupang City has the highest Incidence of DHF in East Nusa Tenggara and also higher than the national figure. This study aims to analyze the relationship between housing conditions and the DHF incidence in the Kupang City. This observational study uses a case-control study in 25 villages with a total sample of 500 people. Data is analyzed using t test and chi square test. The house conditions were significantly related to the DHF incidence (p≤0.05), namely house lighting, water usage, wall construction, ventilation area and ownership of clean water facilities. People are advised to ventilate the house ≥10% of the …

Risk Factor Analysis Of Overnutrition Among Elementary School Children In Pekanbaru: An Urban-Rural Perspective, Arfianti Arfianti

Kesmas

The prevalence of childhood overnutrition is increasing in many parts of the world including Indonesia. National basic health research (RISKESDAS) 2010 showed that over-nutrition in urban children (10.4%) was greater than in rural areas (8.1%). The purpose of this study was to analyse risk factors for overnutrition on elementary students in urban and rural areas in Pekanbaru. This was an observasional analytical study with cross-sectional design. This study involved 137 urban and 113 rural students from six elementary schools in Pekanbaru recruited by quota sampling technique. This study was conducted between April-May 2018. The results showed that the proportions of …

Occurrence Of Natural Vertical Transmission Of “Zika Like Virus” In Aedes Aegypti Mosquito In Jambi City, Tri Baskoro Satoto, Nur Alvira Pasca Wati, Wida Purwaningsih, Hari Kusnanto Josef, Purwono Purwono, Rumbiwati Rumbiwati, Hermanto Hermanto, Roger Frutos

Kesmas

Zika virus can be transmitted through mosquitoes such as Aedes aegypti and Ae. albopictus. During the transition period of 2014–2015, an outbreak of dengue was reported in Jambi City, during which several sufferers were screened positive for Zika virus infection by the Eijkman Institute. It was interesting to note that all of those positive for Zika virus infection were indigenous residents and none of them had a history of international travel. This descriptive analytic study with a cross-sectional design study was conducted to present an overview of Aedes spp. population using ovitrap and egg colonization methods and to detect the …

Factors Related To Adolescent Behavior Towards Hiv/Aids Prevention, Yuliasti Eka Purnamaningrum, Ratyas Ekartika Puspita Candra Nugrahawati, Munica Rita Hernayanti, Anne Vajee

Kesmas

In 2016 Special Region of Yogyakarta was ranked 9th as the province with the highest number of people suffered from HIV/AIDS especially at Sleman regency. Globally, AIDS was the second leading cause of adolescents’s death aged of 10-19 years. The purpose of this study was to find out factors affecting the behaviour of the adolescents towards HIV/AIDS prevention. This research using with cross sectional study design. The sampling technique which used was stratified random sampling resulted in 59 respondents from 11th grade students at SMA Negeri 2 Sleman were selected as sample. Data were collected using questionnaire and analyzed using …

Kajian Narrative Terhadap Profil Farmakokinetik Antibiotik Pada Pasien Kritis: Implikasi Terhadap Ketercapaian Target Farmakokinetik-Farmakodinamik, Eko Setiawan, Widyati, Ferdy Royland Marpaung, Edy Sukandar, Susaniwati, Dwi Lily Lukas, Heru Wijono, Taufin Warindra, Roni Kurniawan, Tjipto Wibowo, Wahyu Hendradi, Menino Osbert Costa, Mohd-Hafiz Abdu Aziz, Jason Roberts

Pharmaceutical Sciences and Research

The severity of diseases, the complexity of treatment, and the use of medical devices in the intensive care unit (ICU) may change the pharmacokinetics (PK) profile of antibiotics among critically ill patients.This narrative review aims to explain the PK profile of critically ill patients compared to other group of patients and to describe the pharmacokinetic-pharmacidynamic (PK-PD) target attainment among this group of patients. Only articles published less than 10 years ago were included in this narrative review. Evidences have indicated that critically ill patients have relatively larger volume distribution (Vd) of hydrophilic antibiotics compared to patients with stable conditions. The …

Profil Of Implementation Of Post Exposure Prophylaxis Of Hepatitis B, Hepatitis C And Human Immunodeficiency Virus To Health Care Worker In Cipto Mangunkusomo Hospital 2014-2016, Dewi Mira Ratih, Evy Yunihastuti, Regina Lestari, Andri Andri Sulaiman, Ikhwan Rinaldi

Jurnal Penyakit Dalam Indonesia

Introduction. Health care workers (HCW) have a high risk of infectious substance exposure. Hepatitis B, hepatitis C, or human immunodeficiency virus (HIV) are some diseases transmitted by body fluid or body tissue. Cipto Mangunkusumo Hospital Jakarta has been implementing post-exposure prophylaxis (PEP)s towards HIV, hepatitis B, and hepatitis C. This study aimed to identify the implementation of post-exposure prophylaxis of HIV, Hepatitis B, and Hepatitis C among HCW in Cipto Mangunkusumo Hospital Jakarta. Methods. A cross-sectional study was conducted to exposed workers in Cipto Mangunkusumo Hospital Jakarta between 2014-2016 who had been recorded by report. Demographic data were collected through …

Perception Of People Living With Hiv/Aids On Social Stigma Of Hiv/Aids In Sukoharjo District, Titik Haryanti, Wartini Wartini

Kesmas

The stigma of wrong society about HIV / AIDS is one of the obstacles to prevention and control of HIV / AIDS in Sukoharjo District. This study aims to determine the relationship of characteristics, causal factors and duration of HIV / AIDS with the perception of people living with HIV to the stigma of society about HIV / AIDS in Sukoharjo District. The research used is analytical descriptive with cross sectional approach. The population is all people with HIV / AIDS until April 2016 amounted to 256 with a sample of 156 people with sampling technique quota sampling. Bivariate analysis …

Influence Of Decentralization And Type Of Patient On Loss To Follow-Up Among Multidrug-Resistant Tuberculosis Patients In Indonesia From 2014 To 2015, Noerfitri Noerfitri, R. Sutiawan, Tri Yunis Miko Wahyono, Pratiwi Ayuningtyas Hartono

Kesmas

Drug-resistant tuberculosis (TB) patients have a greater risk of loss to follow-up (LTFU) than drug-sensitive TB patients, due to their longer treatment duration. This study aimed to determine the influence of decentralization and patient type on LTFU among multidrug-resistant TB (MDR-TB) patients in Indonesia. A retrospective cohort study was conducted at all MDR-TB treatment healthcare facilities in Indonesia from 014 to 2015. Using total sampling technique, 961 patients were examined and sampled. Of these patients, 86.03% were decentralized. Patients were classified into types as follows: 35.17% were “relapse” patients, 5.52% were “new,” 13.94% were classified as “after LTFU” patients, 23.10% …

Knowledge And Practice In Household Waste Management, Agnes Fitria Widiyanto, Suratman Suratman, Nisrina Alifah, Tri Murniati, Oktafiani Catur Pratiwi

Kesmas

Declining environmental quality is one of population caused by household consumption behavior. Some of the highest contaminant contributions are domestic waste, waste, and company waste. Waste contamination will lead to dead fish, decreased water quality and disease transmission. Community approach can be done to solve the waste problem, especially at the household level. This study uses quantitative methods to analyze the effect of intervention and waste management training on changes in household waste management knowledge and practices. This type of research uses quasi experiment with one group of pre and post test design. The results showed that there was an …

Exclusive Breastfeeding And Decrease Of Upper Respiratory Infection Incidence Among Infants Aged 6-12 Months In Kampar District, Riau Province, Musfardi Rustam, Renti Mahkota, Nasrin Kodim

Kesmas

Upper Respiratory Infection (URI) is a major cause of morbidity and mortality of infants and toddlers in developing countries. The high infant morbidity and mortality rates in Indonesia are associated with the low exclusive breastfeeding ability. Breast milk is a natural drink for newborns in the first month of life that is beneficial not only for the babies, but also for mothers. The aim of study was to determine exclusive breastfeeding and decrease in incidence of URI among infants aged 6-12 months. This study was conducted by using case control design. Samples were taken by using cluster random sampling. Subject …

The Many Benefits Of Urban Green Spaces, Junainah Abu Kasim, Mohd Johari Mohd Yusof, Helmi Zulhaidi Mohd Shafri

CSID Journal of Infrastructure Development

Recently, the ideology of greenery city has received global attention which aimed to address the issues of rapid urbanization, population growth and climate change. Urban green spaces (UGS) seem as the most essential asset that could help cities to mitigate the adverse effects of rapid urbanization and urban sprawl in a sustainable way. It plays a vital role in our cities as an urban lung, discharging oxygen to reduce the city’s heat, the wall for harmful air pollution and offer greatest benefits in term of environment, social and economy to a city. However, the presence of UGS as one of …

Comparison Of Absolute Neutrophil Count Between Hospital And Community Acquired Methicillin-Resistant Staphylococcus Aureus Infection, Kurniyanto Kurniyanto, Widayat Djoko Santoso, Leonard Nainggolan, Juferdy Kurniawan

Jurnal Penyakit Dalam Indonesia

Introduction. The virulence factors from community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) mainly due to toxins like Panton Valentin Leukocidin (PVL) and Phenol Soluble Modulin (PSM). Both of toxins cause decrease of value through neutrophil lysis. This study aimed to identify different value of absolute neutrophil count between hospital and community acquired MRSA. Methods. A cross sectional was conducted which included subjects who were infected by MRSA and hospitalized during 2012-2017. Classification of MRSA were divided due to its sensitivity and resistance to non-beta lactam antibiotics. Isolate that resistance to ≤ 2 antibiotics were classified as CA-MRSA. The others with resistance …

Health Care Service For Leprosy Patients In Sitanala Tangerang (A Proposed Approach Of Comprehensive Health Care For Leprosy Patient), Yunia Irawati, Yeni Dwi Lestari, Anna Puspitasari Bani, Sri Linuwih Menaldi, Luh Karunia Wahyuni, Dewinta Retno Kurniawardhani

ASEAN Journal of Community Engagement

Based on WHO data, Indonesia has the third largest leprosy burden in the world, afterIndia and Brazil. Although leprosy was declared to be eliminated in severalprovinces, there are still several leprosy settlements spread in Indonesia. Oneof these settlements is Neglasari village in Tangerang City, West Java, whereleprosy patients have been living since 1981. There is a scarcity ofinformation regarding the features of leprosy in Indonesia, especially aboutthe description of the patients’ posttreatment condition in terms of bothclinical sequelae and health services provided. A collaboration betweenmultiple departments of Ophthalmology, Dermatovenereology, and MedicalRehabilitation from the Cipto Mangunkusumo Hospital/Universitas Indonesia,Jakarta, was established in …

Cumulative Exposure To Solar Ultraviolet A & B Increases Apoptosis Of Peripheral Blood Cutaneous Lymphocyte Antigen (Cla)+ T-Lymphocytes In Outdoor Workers, Afif Nurul Hidayati, Saut Sahat Pohan, Widodo J. Pudjirahardjo, Isaac Effendy

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Exposure to ultraviolet A & B (UVA-UVB) plays a role in the survival of human life, but it may cause negative effects, such as immunosuppression and skin cancer. The effect of solar UVA-UVB exposure on apoptosis (Bax/Bcl-2 ratio) of peripheral blood cutaneous lymphocyte antigen (CLA)+ T-lymphocyte; the immune competent cells in the skin, has not been investigated. Apoptosis of peripheral blood CLA+ Tlymphocyte affects its function; which serves as the skin's resistance and is involved in infectious diseases, skin inflammation, and malignancies. This study observed the effect of solar UVA-UVB to apoptosis (Bax/Bcl2 ratio) of peripheral blood CLA+ …

Faktor-Faktor Yang Berhubungan Dengan Kesuksesan Kesembuhan Dari Pengobatan Regimen Pendek (Short Treatment Regiment) Pada Pasien Tuberkulosis Resistensi Obat Di Indonesia Tahun 2017, Rina Agustina, Rizka Maulida, Yovsyah Yovsyah

Jurnal Epidemiologi Kesehatan Indonesia

TB RO menyebabkan beban pengendalian penyakit TB menjadi bertambah. Adanya penurunan angka keberhasilan pengobatan dari tahun 2010 sebesar 67,9% menjadi 51,1% di tahun 2013 dan peningkatan kasus pasien putus berobat mendorong Indonesia menerapkan pengobatan jangka pendek untuk meningkatkan angka keberhasilan pengobatan TB RO dan menurunkan kasus pasien putus berobat. Penelitian ini bertujuan untuk melihat status kesuksesan kesembuhan dari pengobatan TB RO dan faktor-faktor yang berhubungan dengan status kesuksesan kesembuhan dari pengobatan regimen pendek di Indonesia tahun 2017 dengan menggunakan data pasien TB RO yang tercatat dalam e-TB manager dari pasien-pasien berusia e” 15 tahun yang telah ada status hasil pengobatan …

Evaluasi Ekonomi Penggunaan Antibiotika Profilaksis Cefotaxime Dan Ceftriaxone Pada Pasien Operasi Seksio Sesarea Di Rumah Sakit X, Echa Aisyah, Mardiati Nadjib

Jurnal Ekonomi Kesehatan Indonesia

A prophylactic antibiotic for patients with cesarean section surgery is highly recommended in the clinical guideline. The use of antibiotics is selected by using criteria safety and cost-effectiveness. This partial economic evaluation in the form of cost and outcome analysis was using alongside observational study method, prospective data were collected from April to May 2017 covering 60 patients with cesarean section surgery who received prophylaxis antibiotics, comparing cost-effectiveness between Cefotaxime and Ceftriaxone. The statistical test showed that there were no differences of characteristics in the 60 patients. The analysis based on the hospital perspective carried out in a public hospital …

Optimization Of Pgex System To Express And Isolate Mycobacterium Tuberculosis Inclusion Body Protein In Combining With Modified Refolding Method, Andriansjah Rukmana, Burhanuddin Burhanuddin, Andi Yasmon

Makara Journal of Science

Antigen sub units for vaccine studies are typically isolated from recombinant proteins in an expression system. However, not all protein expression systems are used to express the specific protein. In this study,we optimized the pGEX system combined with the modified protein refolding to express and isolate M. tuberculosis proteins, especially proteins that are expressed as an inclusion body. Resuscitation promoting factor B (RpfB) protein is one of the Resuscitation promoting factor (Rpf) family of proteins that has been studied for its ability to induce cellular immunity in animal tests. Silico analyses demonstrate how RpfB is included in cell wall and …

Molecular Detection For Japanese Encephalitis And Chikungunya Virus As Etiology Agent For Dengue-Like Fever Symptoms, Rizka Ariani, Tjahjani M. Sudiro, Beti E. Dewi

Makara Journal of Health Research

Background: Acute fever with dengue-like fever symptoms with headache, rash, arthritis, perifer bleeding like ptechie and rinhorrhea are symptoms that often complained by patient and it may be caused by arboviruses infection. Arboviruses are very endemic in Indonesia, especially Dengue Virus (DENV), Japanese Encephalisitis Virus (JEV), and Chikungunya Virus (CHIKV) that causing similar symptoms. Molecular detection is very important to confirm the etiology for adequate management. Methods: This study investigated the etiological agent using one step real time RT reverse transcription polymerase chain reaction (RT PCR) method and SYBR Green I as fluorescent binding dye reporter. The viruses that were …