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Empowering Edge Intelligence Through Reparameterized Lightweight Transformers And Distributed Inference, Hosein Esmaeili, Mohammad Ali Afshar Kazemi, Reza Radfar, Nazanin Pilevari
Empowering Edge Intelligence Through Reparameterized Lightweight Transformers And Distributed Inference, Hosein Esmaeili, Mohammad Ali Afshar Kazemi, Reza Radfar, Nazanin Pilevari
Turkish Journal of Electrical Engineering and Computer Sciences
Deploying advanced transformer-based models on resource-constrained edge devices remains a significant challenge due to their high memory footprint and substantial compute requirements. In this paper, we propose a reparameterized transformer framework that integrates High-Rank Factorization (HRF) during training, layer merging at inference, and dynamic, load-balanced distributed inference across multiple devices. To further reduce resource usage, our framework supports mixed-precision quantization down to 4-bit, enabling flexible accuracy–latency–energy trade-offs. Experimental evaluations on the ESC-50 environmental sound dataset demonstrate that our method matches or exceeds the performance of larger baseline models while using 20–30% fewer parameters, achieving up to 48% latency reduction in …
Measurement-Aware Zero-Phase Iterative Learning Control For Robust Regulation Of Nonideal Boost Converters In Electric Vehicle Fast Charging, Aytaç Altan, Mohammed S. Alzaidi, Cağfer Yanarateş
Measurement-Aware Zero-Phase Iterative Learning Control For Robust Regulation Of Nonideal Boost Converters In Electric Vehicle Fast Charging, Aytaç Altan, Mohammed S. Alzaidi, Cağfer Yanarateş
Turkish Journal of Electrical Engineering and Computer Sciences
Integrating battery energy storage with DC-DC boost converters for electric vehicle fast charging exposes the regulator to ageing-induced parameter drift, periodic load pulses, and, critically, the nonidealities in the output-voltage sensing chain. This paper proposes a measurement-aware, zero-phase iterative learning control scheme for robust output-voltage regulation of a nonideal boost converter whose parameters are matched to those of a commercial Texas Instruments TPS6102x battery regulator. The controller combines an inner proportional-integral stabilizing loop with an outer zero-phase learning law that updates a feedforward correction based on the sensor-captured output trajectory; a forward-backward robustness filter suppresses the amplification of measurement noise …
Cover And Contents
Turkish Journal of Electrical Engineering and Computer Sciences
No abstract provided.
A Binary Multiobjective Hippopotamus Optimization Algorithm For Feature Selection In Phishing Website Detection, Fatima Belmessaoud, Sofiane Maza, Djaafar Zouache
A Binary Multiobjective Hippopotamus Optimization Algorithm For Feature Selection In Phishing Website Detection, Fatima Belmessaoud, Sofiane Maza, Djaafar Zouache
Turkish Journal of Electrical Engineering and Computer Sciences
Phishing website detection remains a major challenge in cybersecurity as attackers continuously develop new techniques to deceive users. Identifying the most informative features from large datasets is essential to improve classification accuracy while reducing computational complexity. Feature selection is therefore widely addressed using metaheuristic optimization techniques due to their flexibility and global search capability. In this study, we propose a Binary Multiobjective Hippopotamus Optimization Algorithm (B-MOHOA) for feature selection in phishing website detection. The proposed method simultaneously optimizes two conflicting objectives: maximizing classification accuracy and minimizing the number of selected features. Unlike many existing studies that mainly focus on transfer …
Range–Angle-Dependent Oam Beamforming With A Concentric Helical Circular Fda, Uğur Yeşi̇lyurt
Range–Angle-Dependent Oam Beamforming With A Concentric Helical Circular Fda, Uğur Yeşi̇lyurt
Turkish Journal of Electrical Engineering and Computer Sciences
Secure and spatially selective wireless transmission requires orbital angular momentum (OAM) beams that are confined to a specific range and angle, rather than propagating indefinitely along the beam axis. In this paper, a concentric helical circular frequency diverse array (CHCFDA) is proposed to generate range–angle-dependent OAM beams without requiring external phase shifters. The helical element positioning inherently provides the necessary interelement phase distribution through physical step height, while logarithmically increasing frequency offsets are applied across concentric rings—and optionally across individual elements—to eliminate range periodicity and achieve a single, well-focused OAM beam exclusively at the target location. Both linear and logarithmic …
Zoonotic Fish Parasites That Pose Risks To Human Health In Türkiye, Ahmet Özer
Zoonotic Fish Parasites That Pose Risks To Human Health In Türkiye, Ahmet Özer
Turkish Journal of Zoology
Fish-borne zoonotic diseases have been documented as the causative agents of considerable problems for humans on a global scale. This paper, for the first time, reveals the current zoonotic parasites of fish from wild and cultured hosts in marine, freshwater, and aquarium environments, as well as in native, endemic, and introduced hosts across different geographical areas and seas in Türkiye, based on published data to date. According to the latest global reports, zoonotic fish parasites in Türkiye are dominated by nematodes (17 species), trematodes (11 species), and cestodes (four species), followed by acanthocephalans (two species) and myxozoans (two species). The …
Toxicokinetics And Multibiomarker Assessment Of Coexposure To Sulfamethoxazole And Titanium Dioxide Nanoparticles In Cyprinus Carpio, Nazish Iftikhar, Rabeea Zafar, Imran Hashmi, Asma Jamil, Christopher Joseph Martyniuk, Munib Ahmed Shafique
Toxicokinetics And Multibiomarker Assessment Of Coexposure To Sulfamethoxazole And Titanium Dioxide Nanoparticles In Cyprinus Carpio, Nazish Iftikhar, Rabeea Zafar, Imran Hashmi, Asma Jamil, Christopher Joseph Martyniuk, Munib Ahmed Shafique
Turkish Journal of Zoology
The combined effects of pharmaceuticals and nanoparticles (NPs) on aquatic organisms remain insufficiently characterized. The present study examined toxicokinetics and multibiomarker responses of Cyprinus carpio (common carp) following exposure to sulfamethoxazole (SMX) and titanium dioxide (TiO₂) NPs. Juvenile fish were treated with SMX (25, 50, and 100 μg/L) alone and in combination with 1.5 mg/L TiO₂ NPs (96 h). TiO₂ NPs had a significant effect on the bioaccumulation of SMX in muscle tissues, with SMX reaching peak concentrations of 2286.54 ng/g in the 100 μg/L SMX + 1.5 mg/L TiO₂ NPs group after 72 h, compared to 1477.2 ng/g with …
Involvement Of Melatonin In The Regulation Of Reactive Oxygen Species Levels In Plants, Bariş Uzi̇lday, Oğuzhan Yilmaz, Tolga Yalçinkaya, Zehra Özgecan Tanyolaç, İsmai̇l Türkan, Rengi̇n Özgür Uzi̇lday
Involvement Of Melatonin In The Regulation Of Reactive Oxygen Species Levels In Plants, Bariş Uzi̇lday, Oğuzhan Yilmaz, Tolga Yalçinkaya, Zehra Özgecan Tanyolaç, İsmai̇l Türkan, Rengi̇n Özgür Uzi̇lday
Turkish Journal of Botany
Abiotic stress conditions impair plant metabolism partly by inducing the excessive production of reactive oxygen species (ROS). Melatonin, a multifunctional indoleamine, has emerged as an important regulator of plant responses to oxidative stress. This review highlights recent findings on the interactions among phytomelatonin, ROS homeostasis, antioxidant metabolism, photosynthetic protection, stress priming, and abiotic stress tolerance. We discuss how melatonin modulates the antioxidative response under diverse stress conditions and evaluate its effects on photosynthetic performance, ROS signaling, and membrane stability. Special attention is given to priming studies showing that melatonin pretreatment can improve stress tolerance. Emerging evidence from plant tissue culture …
Drought Priming Improves Waterlogging Tolerance In Alfalfa, But Less Effectively Than Waterlogging Priming, Gürkan Demi̇rkol, Ni̇lüfer Kara
Drought Priming Improves Waterlogging Tolerance In Alfalfa, But Less Effectively Than Waterlogging Priming, Gürkan Demi̇rkol, Ni̇lüfer Kara
Turkish Journal of Botany
Waterlogging severely restricts growth and productivity in alfalfa (Medicago sativa L.), necessitating effective strategies to improve stress tolerance. This study investigated whether drought priming or waterlogging priming could enhance tolerance to subsequent waterlogging stress in the alfalfa cultivar “Bilensoy-80”. Plants were subjected to drought priming, waterlogging priming, or no priming prior to progressive waterlogging stress for 0, 4, 8, 12, 16, and 20 days. Waterlogging priming consistently alleviated growth inhibition, maintained chlorophyll content, and reduced oxidative damage under prolonged stress. Drought priming was associated with partial cross-tolerance, particularly through improved root growth, plant water status, and oxidative stress responses, although …
On Families Of Finsler Metrics, İsmai̇l Sağlam, Ken'ichi Ohshika, Athanase Papadopoulos
On Families Of Finsler Metrics, İsmai̇l Sağlam, Ken'ichi Ohshika, Athanase Papadopoulos
Turkish Journal of Mathematics
In this paper, we answer some natural questions concerning symmetrisation and more general combinations of Finsler metrics, with a view to applications to Funk and Hilbert geometries and metrics on Teichmüller spaces. The metrics on Teichmüller space that we consider are the Thurston metric and the earthquake metric, both introduced by Thurston. The first metric has been thoroughly studied over the last couple of decades, and the second over the last few years. The Funk metric and its symmetrisation, the Hilbert metric, are classical metrics that have been investigated in the contexts of hyperbolic geometry and geometric function theory and, …
A Note On The Periodic Orbits Of Wolbachia Spread Dynamics In Mosquito Populations In Periodic Environments, Jose S. Cánovas
A Note On The Periodic Orbits Of Wolbachia Spread Dynamics In Mosquito Populations In Periodic Environments, Jose S. Cánovas
Turkish Journal of Mathematics
We consider the periodic model introduced by B. Zheng and J. Yu, and disprove the conjectures on the number of periodic orbits the model can have. We rebuild the conjecture to prove that for periodic sequences of maps of any period, the number of nonzero periodic trajectories is bounded by two.
Characterizations Of Bi-Riordan Arrays, Nai̇m Tuğlu, Fatma Yeşi̇l Baran
Characterizations Of Bi-Riordan Arrays, Nai̇m Tuğlu, Fatma Yeşi̇l Baran
Turkish Journal of Mathematics
In this study, we introduce the concept of a bi-Riordan array. As part of this construction, we define new composition and power operations. We then establish an analogue of the well-known fundamental theorem of Riordan arrays (FTRA), which we call the fundamental theorem of bi-Riordan arrays. Next, we show that the product of two bi-Riordan matrices is again a bi-Riordan matrix. Using this product structure, we prove that the set of bi-Riordan matrices forms a monoid.
Exponential Stability For A Strongly Damped Wave Equation With Distributed Internal Delay, Manal Alotaibi, Nasser-Eddine Tatar, Waled Al-Khulaif
Exponential Stability For A Strongly Damped Wave Equation With Distributed Internal Delay, Manal Alotaibi, Nasser-Eddine Tatar, Waled Al-Khulaif
Turkish Journal of Mathematics
This paper investigates the exponential stability of a strongly damped wave equation subject to an internal distributed time delay. Two distinct analytical frameworks are developed: the first employs a modified energy method based on auxiliary functionals, while the second introduces a transformation that rewrites the delay term as the derivative of a convolution integral. Both approaches yield explicit decay conditions and establish exponential convergence of the energy under weaker assumptions on the damping coefficient and the delay kernel. Notably, the transformation method allows for a wider class of admissible delay weights than those permitted by classical techniques. Numerical simulations based …
A Delayed Siamr Prostate Cancer Model With Vertical Transmission And Treatment: Stability Analysis And Numerical Simulation, Hüseyi̇n Demir, İnci̇ Çi̇li̇ngi̇r Süngü, İbrahi̇m Keles
A Delayed Siamr Prostate Cancer Model With Vertical Transmission And Treatment: Stability Analysis And Numerical Simulation, Hüseyi̇n Demir, İnci̇ Çi̇li̇ngi̇r Süngü, İbrahi̇m Keles
Turkish Journal of Mathematics
In this paper, we propose and analyse a delayed-compartmental mathematical model of prostate cancer dynamics that incorporates vertical transmission and therapeutic intervention. The population is stratified into five compartments: susceptible (S), infected/virally activated (I), asymptomatic infectious under treatment (A), malignant untreated (M), and recovered (R). A discrete time delay τ > 0 is introduced in the force-of-infection term to model the age-dependent onset of susceptibility in middle-aged adult males. Vertical transmission of viral etiology at a rate qΛ ensures that a fraction of newborns enter the infected compartment directly, rendering the disease persistent regardless of the basic reproduction number R₀. We …
Hyers-Ulam And Hyers-Ulam Rassias Stability Of Caputo Fractional Hahn Difference Equations, Karima Mohamed Oraby, Alaa E. Hamza, Afrah Al-Bossly
Hyers-Ulam And Hyers-Ulam Rassias Stability Of Caputo Fractional Hahn Difference Equations, Karima Mohamed Oraby, Alaa E. Hamza, Afrah Al-Bossly
Turkish Journal of Mathematics
In this paper, we investigate Hyers–Ulam and Hyers–Ulam–Rassias stability for fractional linear Hahn difference equations of Caputo Type. To the best of our knowledge, this is the first work concerning Hyers–Ulam type stability in the framework of Caputo fractional Hahn difference equations, thereby filling a significant gap in the literature on fractional stability theory. Our approach is based on establishing an equivalence between the fractional Hahn difference initial value problem and a corresponding fractional integral equation, via the Caputo–Hahn inversion formula. These results lay a strong groundwork for analyzing the stability of Hahn fractional systems, which could be useful in …
Stochastic Control Of Inventory And Scheduling Decisions In An Open Network Of Repairables, Erhun Özkan
Stochastic Control Of Inventory And Scheduling Decisions In An Open Network Of Repairables, Erhun Özkan
Turkish Journal of Mathematics
We study maintenance operations for capital goods with repairable components. Because the downtime of a capital good is costly, the maintenance provider keeps an inventory of spare parts to quickly replace broken parts of the capital goods. After the replacement, the broken part is added to the repair facility’s repair queue. Upon a part breakdown, if there is no spare part in the inventory, then the maintenance provider has two options: (i) it can backorder the spare part demand, (ii) it can execute an emergency repair, which is done immediately and quickly, and is followed by a quick installation of …
A Full-Scale Watertight Workflow Numerical Study For A Triangular Fin And Tube Heat Exchanger, Hamdi̇ Selçuk Çeli̇k, Bahadir Doğan, Lati̇fe Berri̇n Erbay
A Full-Scale Watertight Workflow Numerical Study For A Triangular Fin And Tube Heat Exchanger, Hamdi̇ Selçuk Çeli̇k, Bahadir Doğan, Lati̇fe Berri̇n Erbay
Turkish Journal of Mathematics
In this study, the thermo-hydraulic characteristics of an air-cooled triangular finned tube heat exchanger were analyzed numerically. The full-scale heat exchanger was modeled using Ansys Fluent watertight workflow, considering water as the primer fluid to precisely investigate the effects of geometrical factors. The effects of fin height, fin width, and fin spacing of the triangular fins on the performance of the heat exchanger were examined using k-ω turbulence model. A basic finned-tube heat exchanger model, which was manufactured as a monolithic structure without tube-fin contact resistance by milling from aluminum 5083 material, was tested in an air tunnel to verify …
Aptamer–Gold Nanoparticle-Based Lab-In-A-Tube Colorimetric Detection Of Malathion: Evaluation With Classical And Digital Readout Techniques, Emi̇ne Güler Çeli̇k
Aptamer–Gold Nanoparticle-Based Lab-In-A-Tube Colorimetric Detection Of Malathion: Evaluation With Classical And Digital Readout Techniques, Emi̇ne Güler Çeli̇k
Turkish Journal of Chemistry
Colorimetric biosensing devices represent an important class of point-of-care (POC) diagnostics due to their ability to provide visible test outputs, their ease of integration with smartphone-based sensing, and their portability for field testing. In the context of agrifood safety, there is a growing need for portable, robust, and cost-effective detection devices. Pesticide residues can be present in a wide range of environmental and food samples, from water resources to fruits and vegetables, resulting in an increasing global demand for on-site testing. Colorimetric assays, when combined with smartphone-based sensing, can effectively address these needs. In this work, the aggregation-induced color change …
Sensitive Electrochemical Sensor For Simultaneous Determination Of Epinephrine And Norepinephrine By Sio2np@Mwcnt-Cooh Nanoparticle-Modified Spces, Merve Yilmaz Çilçar, Meli̇ke Bi̇lgi̇ Kamaç, Ayşenur Yilmaz Kabaca, Şahi̇n Toparli, Faruk Terzi̇oğlu, Emi̇r Ateş
Sensitive Electrochemical Sensor For Simultaneous Determination Of Epinephrine And Norepinephrine By Sio2np@Mwcnt-Cooh Nanoparticle-Modified Spces, Merve Yilmaz Çilçar, Meli̇ke Bi̇lgi̇ Kamaç, Ayşenur Yilmaz Kabaca, Şahi̇n Toparli, Faruk Terzi̇oğlu, Emi̇r Ateş
Turkish Journal of Chemistry
Epinephrine (EP) and norepinephrine (NEP), both neurotransmitters, are two important biomolecules that play critical roles in the body, from the central nervous system to metabolic processes. They are also important biomarkers for clinical diagnosis and monitoring. In this study, a novel, fast, practical, and high-performance electrochemical sensor based on a SiO2NP@MWCNT-COOH nanocomposite was developed for the simultaneous determination of EP and NEP. The nanocomposite was modified onto the surface of screen-printed electrodes (SPCEs) and its characterization was performed (SEM-EDX, XRD, FTIR, CV, DPV, and EIS). The individual and simultaneous determinations of EP and NEP were performed using square-wave voltammetry, yielding …
Acetylcholinesterase Immobilization On A Natural Thin Membrane For Stable Biocatalyst Design, Si̇nem Öztürk, Ceyhun Işik, Mustafa Teke
Acetylcholinesterase Immobilization On A Natural Thin Membrane For Stable Biocatalyst Design, Si̇nem Öztürk, Ceyhun Işik, Mustafa Teke
Turkish Journal of Chemistry
This study involved the immobilization of acetylcholinesterase (AChE) onto a natural thin membrane extracted from the inner epidermal layer of Allium cepa, with the aim of enhancing the enzyme’s stability, activity, and reusability for potential biotechnological applications. The membrane, composed of biocompatible polymers and naturally occurring functional groups, was used as a sustainable support in a combined strategy involving adsorption followed by glutaraldehyde-mediated cross-linking. Immobilization conditions were optimized by evaluating key factors such as enzyme concentration, membrane quantity, cross-linker dosage, and contact time to achieve maximal catalytic efficiency. Structural and morphological changes on the membrane surface were assessed via scanning …
Folic Acid-Targeted Polymeric Complex Imprinted With Doxorubicin And Quercetin For Controlled Drug Delivery In Cancer Therapy, Ceren Türkcan, Emi̇rhan İşeri̇
Folic Acid-Targeted Polymeric Complex Imprinted With Doxorubicin And Quercetin For Controlled Drug Delivery In Cancer Therapy, Ceren Türkcan, Emi̇rhan İşeri̇
Turkish Journal of Chemistry
Cancer therapy is often limited by the systemic toxicity and dose-dependent adverse effects of conventional chemotherapeutic agents such as doxorubicin (DOX). In this study, a folic acid-functionalized molecularly imprinted polymeric complex based on poly(HEMA) crosslinked with ethylene glycol dimethacrylate was synthesized for the controlled release of DOX in combination with quercetin (QUE), a flavonoid known for its antioxidant and chemosensitizing properties. The aim of this work was to develop and physicochemically characterize a preliminary targeted drug delivery platform at a material-design level. The polymeric complex was synthesized via free-radical polymerization following a DOX–QUE precomplex formation step. Structural characterization was performed …
Electrochemical Determination Of Terbutaline Using A Sno2 Nanoparticle-Modified Carbon Paste Electrode, Rauf Gulamov, Şenol Alpat, Si̇bel Kilinç Alpat
Electrochemical Determination Of Terbutaline Using A Sno2 Nanoparticle-Modified Carbon Paste Electrode, Rauf Gulamov, Şenol Alpat, Si̇bel Kilinç Alpat
Turkish Journal of Chemistry
Terbutaline hemisulfate (THS), a selective beta-2 agonist, is preferred as a bronchodilator for the treatment of asthma and bronchitis, as well as a tocolytic agent to delay labor in acute situations during premature birth. The use of beta-2 agonists above a certain dose is considered doping in sports competitions. Therefore, it is important that they be determined with high sensitivity and selectivity within a short analysis time. In this study, a SnO2 nanoparticle-modified carbon paste electrode was developed for the determination of THS. Cyclic voltammetry and differential pulse voltammetry were employed as electrochemical methods. The operating conditions of the developed …
Chitosan Microparticle-Based Antibacterial Nasal Barrier Formulation And In Vivo Evaluations, Güli̇z Ak, Ayça Erek, Tuğba Karakayali, Şadi̇ye Çeti̇n, Şenay Şanlier
Chitosan Microparticle-Based Antibacterial Nasal Barrier Formulation And In Vivo Evaluations, Güli̇z Ak, Ayça Erek, Tuğba Karakayali, Şadi̇ye Çeti̇n, Şenay Şanlier
Turkish Journal of Chemistry
Microorganisms remain a major cause of infectious diseases, emphasizing the need for alternative and complementary antimicrobial strategies. In this context, plant-derived materials have attracted increasing attention for their antimicrobial and protective properties. Chitosan, a biocompatible and cationic biopolymer, has emerged as an effective carrier system for encapsulating such plant-derived components. In this study, chitosan microparticles were prepared through ionic gelation followed by spray drying and loaded with thyme oil and olive leaf extract. The resulting microparticles exhibited an average size of approximately 1.8 μm and a total phenolic content of 23 mg GAE/g. These microparticles were incorporated into a glycerin–starch …
A Poly-L-Lysine-Functionalized Electrochemical Immunosensor Enables Highly Sensitive Detection Of Tumor Necrosis Factor Receptor-Associated Protein 1, Berfi̇n Vural, Mustafa Kemal Sezgi̇ntürk
A Poly-L-Lysine-Functionalized Electrochemical Immunosensor Enables Highly Sensitive Detection Of Tumor Necrosis Factor Receptor-Associated Protein 1, Berfi̇n Vural, Mustafa Kemal Sezgi̇ntürk
Turkish Journal of Chemistry
Tumor necrosis factor receptor-associated protein 1 (TRAP-1), a member of the mitochondrial heat shock protein family (HSP90), is essential for protein homeostasis, stress response, and cell cycle. TRAP-1 not only optimizes cellular energy production but also prevents cell death, thereby supporting the survival and proliferation of tumor cells. In addition to cancer, TRAP-1 has been associated with Parkinson’s disease, certain autoimmune disorders, and abnormalities in the kidney and urinary tract. In this study, a unique and useful indium tin oxide-polyethylene terephthalate (ITO–PET)-based electrochemical biosensor is described for detecting the biomarker TRAP-1. The ITO–PET electrode, selected as the working electrode, is …
Profiling Arbutin As A Potent Cholinesterase And Gsk-3Β Inhibitor And Evaluating Its Cytotoxicity In Sh-Sy5y Cells For Alzheimer’S Disease Therapy, Shirin Tarbiat, Tuğba Bal, Deni̇z Gülmez
Profiling Arbutin As A Potent Cholinesterase And Gsk-3Β Inhibitor And Evaluating Its Cytotoxicity In Sh-Sy5y Cells For Alzheimer’S Disease Therapy, Shirin Tarbiat, Tuğba Bal, Deni̇z Gülmez
Turkish Journal of Biology
Background/aim: Alzheimer’s disease (AD) is a neurodegenerative disorder linked to cognitive decline and memory loss, marked by hyperphosphorylated tau tangles and decreased acetylcholine levels, which are affected by acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity. Glycogen synthase kinase-3 beta (GSK-3β) also plays a role in AD through abnormal activation. In this study, the potential of β-arbutin to inhibit cholinesterases and GSK-3β was investigated, utilizing in vitro assays, cytotoxicity analysis, and molecular docking simulations to explore its therapeutic implications.
Materials and methods: Ellman’s technique was used to test the inhibitory effects of arbutin on cholinesterase enzymes. An ATP-Glo luminescent assay kit was …
Probiotic Intervention In Colorectal Cancer: The Cytotoxic And Apoptotic Potential Of Lactobacillus Cell-Free Supernatants, Bahar Yilmaz, Berna Erdal
Probiotic Intervention In Colorectal Cancer: The Cytotoxic And Apoptotic Potential Of Lactobacillus Cell-Free Supernatants, Bahar Yilmaz, Berna Erdal
Turkish Journal of Biology
Background/aim: Failures in the treatment of colorectal cancer (CRC) have increased scientific interest in the potential of the bioactive compounds contained in cell-free supernatants (CFSs) obtained from probiotic bacteria for cancer treatment. The aim in this study was to investigate the effects of CFSs obtained from Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus) and Lactobacillus paracasei subsp. paracasei (L. paracasei) on colorectal cancer cell lines (HT-29 and Caco-2) and a healthy colon epithelial cell line (CCD841 CoN).
Materials and methods: Cytotoxic effects on the cell lines were determined by MTT assay. In line with the IC50 values obtained, morphological changes were …
Discovery And Functional Characterization Of Novel Programmed Death-Ligand 1 Monoclonal Antibodies, Nurşah Ersezen, Mai̇de Şeker, Arzu Aysan, Durmuş Akdoğan, Mehmet Ender Avci, Asli Kurden Pekmezci̇, Si̇bel Kalyoncu Uzunlar, Mehmet İnan, Tamer Yağci
Discovery And Functional Characterization Of Novel Programmed Death-Ligand 1 Monoclonal Antibodies, Nurşah Ersezen, Mai̇de Şeker, Arzu Aysan, Durmuş Akdoğan, Mehmet Ender Avci, Asli Kurden Pekmezci̇, Si̇bel Kalyoncu Uzunlar, Mehmet İnan, Tamer Yağci
Turkish Journal of Biology
Background/aim: Targeted therapies with monoclonal antibodies provide cancer patients with better prognosis and diseasefree survival. The blockade of immune checkpoints, including programmed cell death protein-1 (PD-1) and its ligand PD-L1, with monoclonal antibodies may boost immune responses against tumors and is regarded as an effective strategy in cancer immunotherapy. We describe the generation of anti-PD-L1 monoclonal antibodies with high affinity and specificity, and we assess their potential for therapeutic use in cancer.
Materials and methods: Hybridomas were selected for PD-L1 specificity and cross-reactivity with other immune checkpoint proteins and PD-L1 orthologs using indirect ELISA. Immunofluorescence and Western blotting assays were …