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Control Of Trialeurodes Vaporariorum (Westwood) Adults On Glasshouse-Grown Cucumbers In Four Different Growth Substrates: An Efficacy Comparison Of Foliar Application Of Steinernema Feltiae (Filipjev) And Spraying With Thiamethoxamn, Ziga Laznik, Dragan Znidarcic, Stanislav Trdan
Control Of Trialeurodes Vaporariorum (Westwood) Adults On Glasshouse-Grown Cucumbers In Four Different Growth Substrates: An Efficacy Comparison Of Foliar Application Of Steinernema Feltiae (Filipjev) And Spraying With Thiamethoxamn, Ziga Laznik, Dragan Znidarcic, Stanislav Trdan
Turkish Journal of Agriculture and Forestry
In a glasshouse experiment, the effectiveness of the entomopathogenic nematode (EPN) Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) was compared with thiamethoxam for the control of adults of the greenhouse whitefly (GWF), Trialeurodes vaporariorum (Westwood) (Homoptera: Aleyrodidae), on slicer cucumbers. In a period from mid June to the end of August (2007 and 2008), cucumbers were grown in 4 different growth substrates: expanded perlite, expanded vermiculite, light expanded clay, and peat. A suspension of the nematode (2500 infective juveniles mL^{-1}) was applied to cucumbers 5 times in 2007 and twice in 2008, whilst an insecticide thiametoxam at the recommended dose (0.7 g …
Molecular Cloning, Characterization, And Homologous Expression Of An Endochitinase Gene From Bacillus Thuringiensis Serovar Morrisoni, Sezer Okay, Gülay Özcengi̇z
Molecular Cloning, Characterization, And Homologous Expression Of An Endochitinase Gene From Bacillus Thuringiensis Serovar Morrisoni, Sezer Okay, Gülay Özcengi̇z
Turkish Journal of Biology
The endochitinase gene (chi3023) of Bacillus thuringiensis (Bt) serovar morrisoni strain 3023 was amplified via polymerase chain reaction (PCR) and cloned in Escherichia coli. The ORF of chi3023 (GenBank Accession Number: DQ993175) consists of 2031 nucleotides encoding a 676-residue protein with a calculated molecular mass of 74.5 kDa and a pI value of 6.0. The amino acid sequence of Chi3023 was compared with previously sequenced Bt chitinases and the phylogenetic relationships among them were determined. The deduced N-terminal 34 amino acids of the premature Chi3023 exhibited a typical signal peptide. The E. coli-Bt shuttle vector pHT315 was used for homologous …
The Effects Of Uv Radiation On Some Structural And Ultrastructural Parameters In Pepper (Capsicum Longum A.Dc.), Siavash Hosseini Sarghein, Jirair Carapetian, Jalil Khara
The Effects Of Uv Radiation On Some Structural And Ultrastructural Parameters In Pepper (Capsicum Longum A.Dc.), Siavash Hosseini Sarghein, Jirair Carapetian, Jalil Khara
Turkish Journal of Biology
The objective of this study was to evaluate the effects on the structure and ultrastructure of pepper plants (Capsicum longum A.DC.) exposed to UV radiation under greenhouse conditions. The plants were grown in a growth chamber under uniform environmental conditions and after 35 days were exposed to UV-A and UV-C radiation for 15 and 8 days, respectively. Changes in root growth were not significant, but shoot growth decreased in UV-R-exposed plants and decreased significantly in UV-C-exposed plants. Leaf area also decreased in UV-R-exposed plants and decreased significantly in UV-C-exposed plants. Root thickness was not affected by UV treatment, but stem …
Biochemical Factors Involved In The Resistance Of Some Smoked Tropical Fish Samples To Insect Attack, Akinola John Akintola, Oluwatoyin Lawal
Biochemical Factors Involved In The Resistance Of Some Smoked Tropical Fish Samples To Insect Attack, Akinola John Akintola, Oluwatoyin Lawal
Turkish Journal of Biology
The role of mineral elements (ppm), protein and oil content (%), and saponification and free fatty acid values in contributing to resistance against insect infestation was investigated in 4 fish species: Clarias gariepinus, Parachuma obscura, Hydrocynus sp., and Malapterus electricus. Smoked fish samples were purchased from a local market in Ilorin, Kwara State, Nigeria, packaged, and maintained at 4 ^°C for 7 days. They were later brought out to acclimatise, and then observed for 4 months for possible natural insect infestation. Next, crushed samples of each species were analysed using conventional chemical analysis in order to assess the role of …
Antioxidant Properties Of Different Extracts Of Black Mulberry (Morus Nigra L.), Türkan Kutlu, Gökhan Durmaz, Burhan Ateş, İsmet Yilmaz, M. Şevket Çeti̇n
Antioxidant Properties Of Different Extracts Of Black Mulberry (Morus Nigra L.), Türkan Kutlu, Gökhan Durmaz, Burhan Ateş, İsmet Yilmaz, M. Şevket Çeti̇n
Turkish Journal of Biology
In vitro antioxidant properties of 3 different extracts of black mulberry (Morus nigra L.) were investigated. Acidified methanol, acidified water, and non-acidified methanol/water solutions were used to prepare extracts. Different solvents caused different protonation on black mulberry anthocyanin structures, which were predicted according to their UV-VIS spectrum. Extracts indicated 2 main peaks at about ~280 and ~520 nm with different peak areas in their UV-VIS spectrum. The rank of extracts' total phenolic content and reducing power values were both found to be in correlation with their absorbance at ~280 nm. Meanwhile the same relationship was observed between DPPH scavenging activity …
Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l
Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l
Turkish Journal of Biology
We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer.
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Turkish Journal of Biology
Detergent compatible alkaline protease production by a newly isolated Bacillus sp.Y from laundry soil was studied under shake flask conditions in growth medium comprised (g/L): peptone, 1; NaCl, 5; skim milk, 100; Na_2CO_3, 4. Different environmental process parameters such as fermentation period, initial pH, incubation temperature, and nutritional parameters such as supplementation of different starch sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Rice flour showed an almost 2.2-fold increase in protease activity followed by wheat flour, which showed a 1.85-fold increase over the basal media. However, supplementation of potato starch, corn starch, and ragi …
Possible Seasonal Variation Of The Fatty Acid Composition From Melanopsis Praemorsa (L., 1758) (Gastropoda: Prosobranchia), From Southeast Anatolia, Turkey, İhsan Eki̇n, Mehmet Başhan, Ridvan Şeşen
Possible Seasonal Variation Of The Fatty Acid Composition From Melanopsis Praemorsa (L., 1758) (Gastropoda: Prosobranchia), From Southeast Anatolia, Turkey, İhsan Eki̇n, Mehmet Başhan, Ridvan Şeşen
Turkish Journal of Biology
Possible seasonal variations of the fatty acid composition (neutral lipid, phospholipid, and total lipid content) of the freshwater snail Melanopsis praemorsa were investigated over the course of a year. Snail body lipids were fractionated by thin layer chromatography. Fatty acid composition of total body lipid, phospholipid, and neutral lipid of whole snail and total lipid of their algal food were analyzed by capillary gas chromatography and gas chromatography-mass spectrometry. The most abundant fatty acids were C16:0, C18:0, C16:1\omega7, C18:1\omega9, C18:2\omega6, and C18:3\omega3 acids in the lipid analyses of the snail and in its food. Odd-numbered fatty acids and C20 polyunsaturated …
Influence Of Salinity On The Growth And Heavy Metal Accumulation Capacity Of Spirodela Polyrrhiza (Lemnaceae), Zeli̇ha Leblebi̇ci̇, Ahmet Aksoy, Fati̇h Duman
Influence Of Salinity On The Growth And Heavy Metal Accumulation Capacity Of Spirodela Polyrrhiza (Lemnaceae), Zeli̇ha Leblebi̇ci̇, Ahmet Aksoy, Fati̇h Duman
Turkish Journal of Biology
Given that the characteristic of free floating plants to uptake metals can have a positive effect on the efficiency of the remediation process of a wetland, it is important to investigate the factors that may impact the process of uptake of metals by the plants. By conducting this present study over a short duration of time, the effects of salinity on the growth, the content of the photosynthetic pigments (chlorophyll a, b, and carotenoid), and heavy metal uptake by Spirodela polyrrhiza were investigated. It was also observed that at a high level of salinity (100 and 200 mM NaCl) the …
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
Turkish Journal of Biology
Prevention and treatment of various degenerative diseases using traditional medicines is increasingly generating interest, especially in geriatric clinical research. This study aimed to evaluate the free-radical scavenging properties and potential to prevent DNA damage (from oxidative stress) of 56 extracts (polar and non-polar) from 14 medicinal plants (from 12 families) used by diverse Indian tribes and ethnic groups. Although widely known for traditional uses, these plants have not been extensively used for clinical purposes. Scavenging of DPPH^{\bullet} radical by all the extracts was evaluated to estimate their antioxidant potential and rank them accordingly. Extracts occupying the top 10 ranks were …
Evaluation Of Antibacterial, Antifungal, Antiviral, And Antioxidant Potentials Of Some Edible Oils And Their Fatty Acid Profiles, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Bi̇lge Şener
Evaluation Of Antibacterial, Antifungal, Antiviral, And Antioxidant Potentials Of Some Edible Oils And Their Fatty Acid Profiles, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Bi̇lge Şener
Turkish Journal of Biology
In the current study, the oils obtained from the nuts of Corylus avellana L. (hazelnut), Arachis hypogea L. (peanut, groundnut), Pinus pinea L. (umbrella nut), and Juglans regia L. (walnut) were tested for their antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and antimicrobial activity against the standard and isolated strains of Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, and Enterococcus faecalis as well as the fungi Candida albicans and C. parapsilosis by microdilution method, in which inhibition was expressed as minimum inhibition concentrations (MICs). Moreover, antiviral activity of the oils was determined using Herpes simplex (HSV) …
The Composition Of The Essential Oils Of Calamintha Pamphylica Subspecies, Sevi̇m Alan, Mi̇ne Kürkçüoğlu, K.H.C. Başer
The Composition Of The Essential Oils Of Calamintha Pamphylica Subspecies, Sevi̇m Alan, Mi̇ne Kürkçüoğlu, K.H.C. Başer
Turkish Journal of Biology
The essential oils obtained by hydrodistillation of the aerial parts of Calamintha pamphylica Boiss. & Heldr. subsp. pamphylica (A,B), subsp. davisii (Quezel & Contandr.) P.H.Davis (C,D,E), and subsp. alanyense S.Alan & Ocak (F) were analyzed using GC/MS. Menthyl acetate (26.2%-40.7%), menthol (13.5%-18.7%), and pulegone (7.7%-13.0%) were the main constituents in samples A and B; menthone (21.7%), pulegone (19.7%), and menthyl acetate (9.8%) were detected in sample C; isomenthyl acetate (16.7%), isomenthone (13.4%), and menthyl acetate (13.0%) were detected in sample D; menthyl acetate (20.9%), menthol (15.4%), and menthone (14.7%) were detected in sample E; and hexadecanoic acid (30.1%), menthyl acetate …
Molecular Diversity Of Thermophilic Bacteria Isolated From Pasinler Hot Spring (Erzurum, Turkey), Ahmet Adigüzel, Kadri̇ye İnan, Fi̇kretti̇n Şahi̇n, Tüli̇n Arasoğlu, Medi̇ne Güllüce, Ali̇ Osman Beldüz, Özlem Bariş
Molecular Diversity Of Thermophilic Bacteria Isolated From Pasinler Hot Spring (Erzurum, Turkey), Ahmet Adigüzel, Kadri̇ye İnan, Fi̇kretti̇n Şahi̇n, Tüli̇n Arasoğlu, Medi̇ne Güllüce, Ali̇ Osman Beldüz, Özlem Bariş
Turkish Journal of Biology
The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. Totally 9 different bacterial strains were selected based on morphological, physiological, and biochemical tests. These strains were characterized by molecular tests including fatty acid and BOX profiles, and 16S rDNA sequence. The data of fatty acid analysis showed the presence of 14 different fatty acids in the 9 bacterial strains examined. Additionally, 5 of these fatty …
Effects Of Plant Growth Promoting Rhizobacteria On Fruit Set, Pomological And Chemical Characteristics, Color Values, And Vegetative Growth Of Sour Cherry (Prunus Cerasus Cv. Kütahya), Hali̇l Karakurt, Recep Kotan, Fati̇h Dadaşoğlu, Rafet Aslantaş, Fi̇kretti̇n Şahi̇n
Effects Of Plant Growth Promoting Rhizobacteria On Fruit Set, Pomological And Chemical Characteristics, Color Values, And Vegetative Growth Of Sour Cherry (Prunus Cerasus Cv. Kütahya), Hali̇l Karakurt, Recep Kotan, Fati̇h Dadaşoğlu, Rafet Aslantaş, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
This study was performed to determine effects of 4 plant growth promoting rhizobacteria (Bacillus subtilis OSU - 142, Bacillus megaterium M - 3, Burkholderia cepacia OSU - 7, and Pseudomonas putida BA - 8) alone and in combinations on fruit set of sour cherry trees (Prunus cerasus L., cv. Kütahya), and to investigate their resulting pomological and chemical characteristics as well as vegetative growth. All the tested bacterial strains alone or some of their combinations have a great potential to increase especially fruit set and plant vegetative growth, and indirectly affect fruit pomological and chemical characteristics. Therefore, they may be …
Isolation And Identification Of Lactic Acid Bacteria From Boza, And Their Microbial Activity Against Several Reporter Strains, Meri̇h Kivanç, Meral Yilmaz, Erdoğan Çakir
Isolation And Identification Of Lactic Acid Bacteria From Boza, And Their Microbial Activity Against Several Reporter Strains, Meri̇h Kivanç, Meral Yilmaz, Erdoğan Çakir
Turkish Journal of Biology
A total of 45 lactic acid bacteria were isolated from 10 different boza samples consumed in Turkey. The isolates were identified with respect to colonial, cellular morphology, and biochemical characters based on API CHL50 (Biomérieux) kit and also automated riboprinting was used to identify of the isolates. These isolates were tested for inhibitory activity against food-borne bacterial pathogens. Antimicrobial effects of these lactic acid bacteria were determined by the agar spot test and also agar diffusion method. As a result of the RiboPrinter® Microbial Characterization System (DuPont Qualicon) lactic acid bacteria isolates having antimicrobial activity (45 isolates) were identified as …
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Turkish Journal of Biology
DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the …
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Turkish Journal of Biology
The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then …
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Turkish Journal of Biology
Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. …
Comparing Optical Sensing Using Slab Waveguides And Total Internal Reflection Ellipsometry, Sofyan A. Taya, Taher M. El-Agez
Comparing Optical Sensing Using Slab Waveguides And Total Internal Reflection Ellipsometry, Sofyan A. Taya, Taher M. El-Agez
Turkish Journal of Physics
The sensitivity of the effective refractive index of slab waveguide sensors to variations in the refractive index of the cladding is compared to that of the ellipsometric parameters. The changes of the effective refractive index of a waveguide and the ellipsometric parameter \Delta , due to the index change of the cladding, were derived and plotted as a function of the guiding layer thickness and with the index of the cladding. It is found that these changes almost have the same overall feature but the ellipsometric parameters showed considerable higher sensitivity than the effective index of the conventional waveguide optical …
Exotic Localized Structures Based On The Symmetrical Lucas Function Of The (2+1)-Dimensional Generalized Nizhnik-Novikov-Veselov System, Emad A-B. Abdel-Salam, Zeid I. A. Al-Muhiameed
Exotic Localized Structures Based On The Symmetrical Lucas Function Of The (2+1)-Dimensional Generalized Nizhnik-Novikov-Veselov System, Emad A-B. Abdel-Salam, Zeid I. A. Al-Muhiameed
Turkish Journal of Physics
Based on the Lucas Riccati method and a linear variable separation method, new variable separation solutions of the (2+1)-dimensional generalized Nizhnik-Novikov-Veselov system are derived. Then, we give a positive answer for the following question: Are there any localized excitations derived by the use of another functions? For this purpose, some attention will be paid to dromion, peakon, multi dromion-solitoff excitations, regular fractal dromions, stochastic fractal dromion structure and combined structures including bell-like compactons, peakon-like compactons and compacton-like semi-foldons based on the golden main. With the help of the modified Weierstrass function, we discuss the stochastic fractal dromion structure both analytically …
Optical Characterization Of Fe^{3+} Doped Cds Nanoparticles Synthesized By Wet-Chemical Route, Seyed Mohammad Taheri Otaqsara, Mohammad Hassan Yousefi, Ali Azam Khosravi
Optical Characterization Of Fe^{3+} Doped Cds Nanoparticles Synthesized By Wet-Chemical Route, Seyed Mohammad Taheri Otaqsara, Mohammad Hassan Yousefi, Ali Azam Khosravi
Turkish Journal of Physics
Fe^{3+} doped CdS nanoparticles using Mercaptoethanol as a capping agent were prepared by colloidal wet-chemical method. UV-VIS spectra show that Fe-doping can effectively tune energy band structure, i.e. a large blue shift (\sim 30 nm) with increasing Fe concentration. PL measurement of doped CdS nanoparticles shows an additional emission band at \sim 532 nm, which can be due to radiative transition of dopant level to ground state. X-ray diffraction patterns studies revealed cubic crystal phase. TEM micrographs show a spherical shape and nearly well distribution with an average particle size of \sim 4 nm.
Histological Examination Of Testicular Cell Development And Apoptosis In The Ostrich Chick, Wei Lan, Ke-Mei Peng, Huazhen Liu, Hui Song, Yang Wang, Li Tang
Histological Examination Of Testicular Cell Development And Apoptosis In The Ostrich Chick, Wei Lan, Ke-Mei Peng, Huazhen Liu, Hui Song, Yang Wang, Li Tang
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to observe the microstructure and ultrastructure of the testis, demonstrate testicular cell development and apoptosis, and elucidate regularity of development and apoptosis in ostrich chick testes. By employing light microscopy 3 obvious development characteristics were detected with ostrich age increasing: first, many primordial germ cells and a few spermatogonia were found while seminiferous tubule integrity was not evident in testes of 1-day-old ostrich chicks nor were primordial germ cells. In the 30-day-old ostrich chicks spermatogonia were completely differentiated, but very few primary spermatocytes were observed in testes at 45 days of age. Second, the …
Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen, Tao Zhang, Shuang Zhang, Li-Fang Tian, Ge Hu, Zhan-Wei Suo, Wu Chen, Hui-Qin Duan, Xiang Mu, Ke-Dao Teng
Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen, Tao Zhang, Shuang Zhang, Li-Fang Tian, Ge Hu, Zhan-Wei Suo, Wu Chen, Hui-Qin Duan, Xiang Mu, Ke-Dao Teng
Turkish Journal of Veterinary & Animal Sciences
To investigate the global response of microvascular endothelial cells (MVECs) to foot-and-mouth (FMDV)disease vaccine (FMDV) antigen and to explore the role of MVECs in FMD vaccine immunity, the gene expression alterations of rat myocardium microvascular endothelial cells (RMMVECs) after stimulation with FMD virus (FMDV) 146S antigen were assayed using cDNA microarrays. We observed that 18 genes and 67 genes in RMMVECs were respectively altered by FMDV 146S antigen at 6 h and 24 h, respectively. The former were all up-regulated genes, and the latter included 60 up-regulated genes and 7 down-regulated genes. Among the differentially expressed genes, many were associated …
Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania, Steliana Elvira Maria Kevorkian, Mihaela Zaulet, Maria Adina Manea, Sergiu Emil Georgescu, Marieta Costache
Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania, Steliana Elvira Maria Kevorkian, Mihaela Zaulet, Maria Adina Manea, Sergiu Emil Georgescu, Marieta Costache
Turkish Journal of Veterinary & Animal Sciences
Ovine scrapie is considered a neurodegenerative disease characterized by the accumulation of an abnormal, protease-resistant isoform of PrP. The polymorphisms at codons 136, 154, and 171 are associated with susceptibility to scrapie. In order to detect the existing polymorphisms at the 3 codons we used the real-time PCR technique. The obtained genotypes were confirmed by PCR amplification and sequencing of a 402 bp fragment of exon 3 of the prion gene. Known polymorphisms were detected at codons 136, 154, and 171 and 5 different genotypes were determined. The results showed that all animals were homozygous for A at codon 136 …
Effects Of Dietary Fish Oil Replacement By Cottonseed Oil On Growth Performance And Fatty Acid Composition Of Rainbow Trout (Oncorhynchus Mykiss), Müjde Güler, Mustafa Yildiz
Effects Of Dietary Fish Oil Replacement By Cottonseed Oil On Growth Performance And Fatty Acid Composition Of Rainbow Trout (Oncorhynchus Mykiss), Müjde Güler, Mustafa Yildiz
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to determine the effects of replacing fish oil (FO) with cottonseed oil (CSO), as an alternative plant lipid source in diets for rainbow trout (89.3 ± 1.1 g mean individual weight), on the growth and fatty acid composition of fish. As a control diet, 5 experimental diets were formulated with pure FO and with partial or complete (25%, 50%, 75%, or 100% CSO, respectively) replacement of FO with CSO. The fish were fed 2% of their body weight per day for 60 days at an average water temperature of 10.2 °C. At the …
The Increase Of Cd4+Cd25+ T Cells In The Peripheral Blood Of Pigs Persistently Infected With Porcine Reproductive And Respiratory Syndrome Virus, Jingwen Zeng, Danting He, Xin Guo, Xinna Ge, Hanchun Yang
The Increase Of Cd4+Cd25+ T Cells In The Peripheral Blood Of Pigs Persistently Infected With Porcine Reproductive And Respiratory Syndrome Virus, Jingwen Zeng, Danting He, Xin Guo, Xinna Ge, Hanchun Yang
Turkish Journal of Veterinary & Animal Sciences
Regulatory T lymphocytes (T-regs) may be involved in suppressing the host's immunity, which is important for viral clearance, and may therefore contribute to persistent viral infection. However, nothing is known regarding the situation of porcine T-regs during persistent infection with porcine reproductive and respiratory syndrome virus (PRRSV). This study, which used flow cytometry and real-time PCR, determined that the frequencies of CD4+CD25+ T-cells and porcine Forkhead/winged helix transcription factor (FoxP3) mRNA levels in peripheral blood significantly increased during PRRSV persistence, indicating that CD4+CD25+ T-regs might play an important role in PRRSV persistent infection. This study contributes to a better understanding …
Effects Of Queen Rearing Period On Reproductive Features Of Italian (Apis Mellifera Ligustica), Caucasian (Apis Mellifera Caucasica), And Aegean Ecotype Of Anatolian Honey Bee (Apis Mellifera Anatoliaca) Queens, Aytül Uçak Koç, Mete Karacaoğlu
Effects Of Queen Rearing Period On Reproductive Features Of Italian (Apis Mellifera Ligustica), Caucasian (Apis Mellifera Caucasica), And Aegean Ecotype Of Anatolian Honey Bee (Apis Mellifera Anatoliaca) Queens, Aytül Uçak Koç, Mete Karacaoğlu
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of rearing period on the quality characteristics of honey bee queens reared from Italian and Caucasian races and from the Aegean ecotype of Anatolian honey bee under the conditions of the Aegean region. Italian (I), Caucasian (C), and Aegean (A) queens were reared in April, June, and August of 2007 and 2008. The queens were weighed on the second day after emergence (WQ) and at the onset of oviposition (OWQ). The interval from emergence to the onset of oviposition (preoviposition period, POP) was determined in 3 genotype queen groups. The numbers of …
Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic
Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic
Turkish Journal of Veterinary & Animal Sciences
Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, …