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Synthesis, Spectroscopic Characterization, And Genotoxicity Of A New Group Of Azo-Oxime Metal Chelates, Beyza Cabi̇r, Bariş Avar, Mehmet Gülcan, Ahmet Kayraldiz, Mükerrem Kurtoğlu
Synthesis, Spectroscopic Characterization, And Genotoxicity Of A New Group Of Azo-Oxime Metal Chelates, Beyza Cabi̇r, Bariş Avar, Mehmet Gülcan, Ahmet Kayraldiz, Mükerrem Kurtoğlu
Turkish Journal of Chemistry
A new azo-oxime ligand, 2-[(E)-(hydroxyimino)methyl]-4-[(E)- (4-nitrophenyl)diazenyl]phenol, (pxoxH_2) (2), was prepared by condensation of hydroxylamminehydrochloride and 2-hydroxy5- [(4-nitrophenyl)diazenyl]benzaldehyde (1) with treatment of a solution of diazonium salt of p-nitroaniline and 2-hydroxybenzaldehyde in ethanol. The 6 coordination compounds, [Mn(pxoxH)_2(H_2O)_2].H_2O (3), [Co(pxoxH)_2(H_2O)_2].4H_2O (4), [Ni(pxoxH)_2(H_2O)_2].2H_2O (5), [Cu(pxoxH)_2].H_2O (6), [Zn(pxoxH)_2].6H_2O (7), and [Cd(pxoxH)_2(H_2O)_2].H_2O (8), were prepared by reacting Mn(II), Ni(II) Cu(II), Co(II), Zn(II), and Cd(II) ions with the ligand. The structures of the compounds were elucidated from the elemental analysis data and spectroscopic studies. The azo-oxime metal complexes were also characterized by XRD, magnetic moment, molar conductivity, and thermal analyses. Elemental analyses of the chelates …
Flavonoid Constituents Of Sideritis Caesarea, Belkis Halfon, Ece Çi̇ftçi̇, Gülaçti Topçu
Flavonoid Constituents Of Sideritis Caesarea, Belkis Halfon, Ece Çi̇ftçi̇, Gülaçti Topçu
Turkish Journal of Chemistry
The acetone extract of the aerial parts of Sideritis caesarea Duman, Aytaç & Başer (Lamiaceae) afforded the flavonoids penduletin (1) and apigenin (2) and 6 glycosylated flavonoids, 4'-O-methyl-isoscutellarein-7-O-[6'''-O-acetyl-\beta-D-allopyranosyl- (1 \to 2)]-6\"-O-acetyl-\beta -D-glucopyranoside (3), 4'-O-methylhypolaetin- 7-O-[6'''-O-acetyl-\beta -D-allopyranosyl-(1 \to 2)]-6\"-O-acetyl-\beta- D-glucopyranoside (4), isoscutellarein-7-O-[6'''-O-acetyl-\beta- D-allopyranosyl-(1 \to 2)]-6\"-O-acetyl-\beta -D-glucopyranoside (5), isoscutellarein-7-O-[6'''-O-acetyl-\beta -D-allopyranosyl-(1 \to 2)]- \beta-D-glucopyranoside (6), 4'-O-methylhypolaetin-7-O- [6'''-O-acetyl-\beta -D-allopyranosyl-(1 \to 2)]-\beta-D- glucopyranoside (7), and hypolaetin-7-O-[6'''-O-acetyl-\beta- D-allopyranosyl-(1 \to 2)]-\beta -D-glucopyranoside (8). The compounds were identified by the use of 1D- and 2D-NMR and UV spectroscopic techniques and by comparisons with the reported data. The acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of the acetone, methanol, …
Quasielastic Light Scattering Studies Of Polypeptides: Evidence For Chain Extension In Solution, Mehmet Bahatti̇n Baysal, Çi̇ğdem Karadağ
Quasielastic Light Scattering Studies Of Polypeptides: Evidence For Chain Extension In Solution, Mehmet Bahatti̇n Baysal, Çi̇ğdem Karadağ
Turkish Journal of Chemistry
Dynamic light scattering and viscosimetric methods were used to study polypeptides including polyleucine, poly (\gamma-benzyl-L-glutamate), poly (\alpha-L-glutamic acid), and polyproline II. Chain extension showing rod-like and flexible conformations occurred in poly(\gamma -benzyl-L-glutamate) and polyproline II solutions as these solutions were diluted in glacial acetic acid or in a mixed solvent of acetic acid and dichloromethane. The elongation of chains in poly(\gamma -benzyl-L-glutamate) and polyproline II solutions was studied by dynamic light scattering and viscosimetric methods. This nonenzymatic growth of peptide chains provides data on primordial synthesis of protein molecules in the absence of cell machinery and for this reason these …
Synthesis Of Fused Tetrazolone Derivatives, Sevi̇l Özcan, Zeynep Ekmekçi̇, Berk Müjde, Meti̇n Balci
Synthesis Of Fused Tetrazolone Derivatives, Sevi̇l Özcan, Zeynep Ekmekçi̇, Berk Müjde, Meti̇n Balci
Turkish Journal of Chemistry
New fused tetrazolone derivatives were synthesized using homophthalic and maleic anhydrides. Treatment of anhydrides with trimethylsilyl azide opened the lactone rings and formed the corresponding intermediates, which bore 1,3-dipole and dipolarophile functionalities in ortho positions. The intermediates partially underwent internal 1,3-dipolar cycloaddition to produce fused tetrazolone derivatives. When the carbonyl groups in anhydride were not conjugated with any double bond, then a triazine-fused tetrazolone derivative was formed.
Ultrasound-Assisted Emulsification--Solidified Floating Organic Drop Microextraction Combined With Flow Injection--Flame Atomic Absorption Spectrometry For The Determination Of Palladium In Water Samples, Shayessteh Dadfarnia, Ali Mohammad Haji Shabani, Mooud Amirkavei
Ultrasound-Assisted Emulsification--Solidified Floating Organic Drop Microextraction Combined With Flow Injection--Flame Atomic Absorption Spectrometry For The Determination Of Palladium In Water Samples, Shayessteh Dadfarnia, Ali Mohammad Haji Shabani, Mooud Amirkavei
Turkish Journal of Chemistry
The ultrasound-assisted emulsification--solidified floating organic drop microextraction (USAE--SFODME) methodology was combined with flow injection--lame atomic absorption spectrometry (FI--FAAS) for the separation/pre\-concentration and determination of palladium at ultratrace level. In this method, the palladium ion in the aqueous solution was complexed with acetylacetone (6 \times 10^{-3} mol L^{-1}) in the pH range of 1--7 and was extracted into 40 \mu L of 1-undecanol, which was sonically dispersed in the aqueous phase. The vial was then centrifuged and cooled in an ice bath for 5 min. The solidified extract was melted and diluted to 100 \mu L with a solution of hydrochloric …
Synthesis And Biological Activities Of Methylenebis-4h-1,2,4-Triazole Derivatives, Yildiz Uygun, Hacer Bayrak, Havva Özkan
Synthesis And Biological Activities Of Methylenebis-4h-1,2,4-Triazole Derivatives, Yildiz Uygun, Hacer Bayrak, Havva Özkan
Turkish Journal of Chemistry
5,5'-Methylenebis(4--phenyl-4H-1,2,4-triazole-3-thiol) (2) was synthesized starting from hydrazinecarbothioamide compound (1). Treatment of compound 2 with ethyl bromoacetate produced diethyl 5,5'-{methylenebis[(4-phenyl-4H-1,2,4-triazole-5,3-diyl)thio]}diacetate (3), which was converted to the corresponding diacetohydrazide derivative (4) by treatment with hydrazine hydrate. The reaction of compound 4 with several aldehydes produced the corresponding arylidene hydrazides, 5a--d. Syntheses of Mannich bases 6a--c were carried out by the treatment of compound 2 with several amines in the presence of formaldehyde. (4{[5-({5-[(4-Amino-2-chlorophenyl)thio]-4-phenyl-4H-1,2,4-triazol-3-ylmethyl)-4-phenyl-4H-1,2,4-triazol-3-yl]thio}-3-chlorophenyl)amine (8) was prepared by reduction of 2 nitro groups of 3,3' -methylenebis{5-[(2-chloro-4-nitrophenyl)thio]-4-phenyl-4H-1,2,4-triazole} (7) that were obtained from the condensation of 2} with 3,4-dichloronitrobenzene. The newly synthesized compounds were screened …
A New Anthracene Derivative Of Calix[4]Arene As A Fluorescent Chemosensor, Özlem Şahi̇n, Mustafa Şahi̇n, Nuri̇ye Koçak, Mustafa Yilmaz
A New Anthracene Derivative Of Calix[4]Arene As A Fluorescent Chemosensor, Özlem Şahi̇n, Mustafa Şahi̇n, Nuri̇ye Koçak, Mustafa Yilmaz
Turkish Journal of Chemistry
A new anthracene derivative of calix[4]arene was synthesized as a highly fluorescent compound. This compound was examined for its fluorescent properties towards different metal ions (Li^+, Na^+, Mg^{2+}, Ca^{2+}, Ba^{2+}, Ni^{2+}, Cu^{2+}, Zn^{2+}, Pb^{2+}) by UV and fluorescence spectroscopy. On complexation by alkaline earth metal cations and transition metal cations, the fluorescence spectrum was quenched. In particular, Ca^{2+} caused greater than 98% quenching of the anthracene derivative of calix[4]arene.
Synthesis, Characterization, And Dft Calculation Of A Pd(Ii) Schiff Base Complex, Alireza Akbari, Zahra Alinia
Synthesis, Characterization, And Dft Calculation Of A Pd(Ii) Schiff Base Complex, Alireza Akbari, Zahra Alinia
Turkish Journal of Chemistry
The 4-((E)-(2-((E)-2, 4-dihydroxybenzylideneamino) ethylimino) methyl) benzene-1,3-diol tetradentate ligand, H_2L, reacted with PdCl_2 to produce the related complex. The complex was characterized by elemental analysis, infrared and electronic spectroscopy, thermogravimetric study, and molar conductance. Furthermore, the fully optimized geometries were calculated using the ADF 2009.01 package. Comparison between the calculated and experimental results covering molecular structures, assignment of fundamental vibrational modes, and thermodynamic properties were investigated. The optimized molecular geometries were compared with the experimental data obtained from X-ray data of a similar complex, which indicated that the theoretical results agree with the corresponding experimental values. The UV-Vis spectrum of the …
Synthesis, Characterization, And Fluorescence Study Of Phthalhydrazidylazo Derivative Of An Unsaturated Diketone And Its Metal Complexes, Radhika Pallikkavil, Muhammed Basheer Ummathur, Krishnannair Krishnankutty
Synthesis, Characterization, And Fluorescence Study Of Phthalhydrazidylazo Derivative Of An Unsaturated Diketone And Its Metal Complexes, Radhika Pallikkavil, Muhammed Basheer Ummathur, Krishnannair Krishnankutty
Turkish Journal of Chemistry
The coupling of diazotized luminol with the unsaturated diketone 1,7-diphenylhepta-4,6-diene-1,3-dione yielded a new type of tridentate ligand (H_2L). Analytical, IR, ^1H NMR, and mass spectral data indicate the existence of the compound in the intramolecularly hydrogen-bonded keto-hydrazone tautomeric form. Dibasic tridentate coordination of the compound in its [ML(H_2O)] complexes [M = Cu(II), Ni(II) and Zn(II)] was established on the basis of analytical and spectral data. The Ni(II) and Zn(II) chelates are diamagnetic while Cu(II) complex showed normal paramagnetic moment. The fluorescence maxima of the compound in different solvents show that the emission wavelength increases with increases in the polarity of …
Comparative Study Of Microwave-Assisted And Conventional Synthesis Of Ibuprofen-Based Acyl Hydrazone Derivatives, Ayşe Uzgören Baran
Comparative Study Of Microwave-Assisted And Conventional Synthesis Of Ibuprofen-Based Acyl Hydrazone Derivatives, Ayşe Uzgören Baran
Turkish Journal of Chemistry
A series of potential biological active acyl hydrazone derivatives containing ibuprofen moiety (compounds 4a--4p) was synthesized by the condensation of ibuprofen hydrazone with aromatic aldehydes using conventional and microwave irradiation methods. The microwave method was found to be successful with nearly the same or higher yields and shorter reaction time, and it was more environmentally friendly compared to the conventional heating method. The chemical structures of the synthesized compounds were characterized by IR, ^1H NMR, and APT-NMR spectroscopy.
Artificial Immunity-Based Induction Motor Bearing Fault Diagnosis, Hakan Çaliş, Abdülkadi̇r Çakir, Emre Dandil
Artificial Immunity-Based Induction Motor Bearing Fault Diagnosis, Hakan Çaliş, Abdülkadi̇r Çakir, Emre Dandil
Turkish Journal of Electrical Engineering and Computer Sciences
In this study, the artificial immunity of the negative selection algorithm is used for bearing fault detection. It is implemented in MATLAB-based graphical user interface software. The developed software uses amplitudes of the vibration signal in the time and frequency domains. Outer, inner, and ball defects in the bearings of the induction motor are detected by anomaly monitoring. The time instants of the fault occurrence and fault level are determined according to the number of activated detectors. Anomaly detection in the frequency domain is implemented by monitoring the fault indicator bearing frequencies and harmonics, calculated using the bearing dimensions and …
System Designs To Perform Bioinformatics Sequence Alignment, Çağlar Yilmaz, Mustafa Gök
System Designs To Perform Bioinformatics Sequence Alignment, Çağlar Yilmaz, Mustafa Gök
Turkish Journal of Electrical Engineering and Computer Sciences
The emerging field of bioinformatics uses computing as a tool to understand biology. Biological data of organisms (nucleotide and amino acid sequences) are stored in databases that contain billions of records. In order to process the vast amount of data in a reasonable time, high-performance analysis systems are developed. The main operation shared by the analysis tools is the search for matching patterns between sequences of data (sequence alignment). In this paper, we present 2 systems that can perform pairwise and multiple sequence alignment operations. Through the optimized design methods, proposed systems achieve up to 3.6 times more performance compared …
A Comparative Review Of Regression Ensembles On Drug Design Datasets, Mehmet Fati̇h Amasyali, Kadri̇ Okan Ersoy
A Comparative Review Of Regression Ensembles On Drug Design Datasets, Mehmet Fati̇h Amasyali, Kadri̇ Okan Ersoy
Turkish Journal of Electrical Engineering and Computer Sciences
Drug design datasets are usually known as hard-modeled, having a large number of features and a small number of samples. Regression types of problems are common in the drug design area. Committee machines (ensembles) have become popular in machine learning because of their good performance. In this study, the dynamics of ensembles used in regression-related drug design problems are investigated with a drug design dataset collection. The study tries to determine the most successful ensemble algorithm, the base algorithm--ensemble pair having the best/worst results, the best successful single algorithm, and the similarities of algorithms according to their performances. We also …
Antimicrobial Susceptibility Patterns Of Environmental And Hospital Isolations Of Enterococci In Aydın, Erman Oryaşin, Haci Hali̇l Biyik, Gamze Başbülbül, Bülent Bozdoğan
Antimicrobial Susceptibility Patterns Of Environmental And Hospital Isolations Of Enterococci In Aydın, Erman Oryaşin, Haci Hali̇l Biyik, Gamze Başbülbül, Bülent Bozdoğan
Turkish Journal of Biology
The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. The results were compared with the susceptibility patterns of hospital enterococci reported in a previous study. Samples from 9 environmental sources were cultured, and species were identified using 16S rRNA sequencing. Antimicrobial susceptibilities were determined by the agar dilution method. The minimum inhibitory concentrations were tested for vancomycin, tetracycline, teicoplanin, ampicillin, gentamicin, erythromycin, rifampicin, clindamycin, chloramphenicol, and ciprofloxacin. Resistance genes and transposons were determined …
Production And Characterization Of Uricase From Streptomyces Exfoliatus Ur10 Isolated From Farm Wastes, Magda Aly, Sanaa Tork, Saleh Al-Garni, Reda Allam
Production And Characterization Of Uricase From Streptomyces Exfoliatus Ur10 Isolated From Farm Wastes, Magda Aly, Sanaa Tork, Saleh Al-Garni, Reda Allam
Turkish Journal of Biology
Uricase plays an important role in nitrogen metabolism and can be used medically as a diagnostic reagent. From soil, wastewater, and poultry waste samples collected in Jeddah, 49 bacterial isolates were obtained on either nutrient agar or starch nitrate agar. All the obtained isolates were screened on minimal medium containing 0.5% uric acid for uricase production. The most active bacterium (isolate UR10) produced about 0.5 U/mL of intracellular uricase and was identified as a species belonging to the genus Streptomyces using morphological, physiological, and biochemical characters. By 16S rDNA, it was identified as Streptomyces exfoliatus UR10. Maximum uricase production was …
Morphological And Molecular Identification Of Pennate Diatoms Isolated From Urla, İzmir, Coast Of The Aegean Sea, Kutsal Kesi̇ci̇, İnci̇ Tüney, Doğuş Zeren, Mustafa Güden, Atakan Sukatar
Morphological And Molecular Identification Of Pennate Diatoms Isolated From Urla, İzmir, Coast Of The Aegean Sea, Kutsal Kesi̇ci̇, İnci̇ Tüney, Doğuş Zeren, Mustafa Güden, Atakan Sukatar
Turkish Journal of Biology
Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological …
Purification And Biochemical Characterization Of An Extracellular Lipase From Psychrotolerant Pseudomonas Fluorescens Ke38, Aysun Adan Gökbulut, Alper Arslanoğlu
Purification And Biochemical Characterization Of An Extracellular Lipase From Psychrotolerant Pseudomonas Fluorescens Ke38, Aysun Adan Gökbulut, Alper Arslanoğlu
Turkish Journal of Biology
An extracellular lipase producing bacterium was isolated from a soil sample, and identified as a strain of Pseudomonas fluorescens by 16S rRNA gene sequencing. It was named Pseudomonas fluorescens KE38. KE38 showed psychrotolerant properties with an optimum growth temperature of 25 °C. The lipase enzyme secreted by KE38 was purified 41.13-fold with an overall yield of 54.99%, and a specific activity of 337.3 U/mg. The molecular mass of purified lipase was estimated to be approximately 43 kDa by SDS-PAGE. Although the lipase was active at a temperature range of 1565 °C, it exhibited maximum activity at 45 °C, at pH …
Effect Of Asiatic And Ursolic Acids On Growth And Virulence Factors Of Uropathogenic Escherichia Coli Strains, Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia
Effect Of Asiatic And Ursolic Acids On Growth And Virulence Factors Of Uropathogenic Escherichia Coli Strains, Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia
Turkish Journal of Biology
This work was aimed at determining the possible antimicrobial potential of asiatic acid (AA) and ursolic acid (UA) against clinical uropathogenic Escherichia coli strains (UPECs). Phylogenetic affiliations of UPECs and virulence-associated gene carriage have been identified. PCR analysis revealed that the studied E. coli strains belong to phylogenetic group B2. All bacterial isolates were grouped into 4 pathotypes. The following were determined: the minimum inhibitory concentrations (MICs) according to CLSI; P-fimbriae synthesis, based on erythrocyte agglutination; curli fibers, by using YESCA agar with congo red; swimming motility, in motility agar; and alpha-hemolysin, on sheeps-blood agar plates. The differences in antigrowth …
Hepatopancreas Mitochondria Of Mytilus Galloprovincialis: Effect Of Zinc Ions On Mitochondrial Bioenergetics And Metabolism, Antonino De Lisi, Ermelinda Prato, Francesca Biandolino, Giulio Sarli, Donatella Negro, Gianluigi La Piana, Domenico Marzulli
Hepatopancreas Mitochondria Of Mytilus Galloprovincialis: Effect Of Zinc Ions On Mitochondrial Bioenergetics And Metabolism, Antonino De Lisi, Ermelinda Prato, Francesca Biandolino, Giulio Sarli, Donatella Negro, Gianluigi La Piana, Domenico Marzulli
Turkish Journal of Biology
Oxygen uptake, respiratory complexes, and metabolic activities have been studied in mitochondria isolated from the hepatopancreata of Mytilus galloprovincialis collected in the Mar Grande of Taranto (Ionian Sea, Italy). Although exposure to 5.0 µg Zn^{2+}/L resulted in a significant increase of states 3 and 4 respiration with glutamate + pyruvate as respiratory substrate, it was found that the exposure of mussels to different concentrations of Zn^{2+} (2.57.5 µg/L) neither inhibited mitochondrial respiration nor exerted any inhibitory effect on representative mitochondrial dehydrogenases. It rather stimulates these activities, producing an extra synthesis of adenosine triphosphate by hepatopancreas mitochondria and possibly increasing its …
Transcriptional Profiling Of Transferrin Gene From Egyptian Cotton Leaf Worm, Spodoptera Littoralis, Nurper Güz, Asli Dağeri̇, Tuğba Erdoğan, Mouzhgan Mousavi, Şeri̇fe Bayram, Mehmet Oktay Gürkan
Transcriptional Profiling Of Transferrin Gene From Egyptian Cotton Leaf Worm, Spodoptera Littoralis, Nurper Güz, Asli Dağeri̇, Tuğba Erdoğan, Mouzhgan Mousavi, Şeri̇fe Bayram, Mehmet Oktay Gürkan
Turkish Journal of Biology
Iron is an essential nutrient for almost all organisms, but it is also a potent toxin because it can catalyze oxidative reactions that are destructive to cells. Transferrin is an essential protein involved in iron metabolism, immunity, and vitellogenesis in insects. We have characterized a cDNA encoding a putative iron-binding transferrin (SpliTrf) in Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae), an important pest that causes extensive losses in many vegetable, fodder, and fiber crops. The structure of the putative SpliTrf showed significant homology to other insect transferrins. Gene expression of SpliTrf was examined from different adult tissues including the …
Activity Of Brassica Napus And Perilla Frutescens Microsomal \Omega-3 Desaturases Expressed In Yeast (Saccharomyces Cerevisiae), Mohammed Abdelreheem, David Hildebrand
Activity Of Brassica Napus And Perilla Frutescens Microsomal \Omega-3 Desaturases Expressed In Yeast (Saccharomyces Cerevisiae), Mohammed Abdelreheem, David Hildebrand
Turkish Journal of Biology
Yeast transformations with Brassica napus and Perilla frutescens \omega-3 desaturases were carried out. Feeding experiments on exogenous 18:2 at 10 mg/L and 100 mg/L for both genes under this investigation showed the same activity levels over the 3 days of the incubation, producing almost the same amount of 18:3. To further investigate the characteristics of B. napus and P. frutescens \omega -3 desaturases, a time-course in vivo activity study was conducted. Both enzymes showed the same behavior over the time course, desaturating 18:2 into 18:3. Moreover, the use of 2 different temperatures for incubation (30 °C and 20 °C) did …
Differential Expression Analysis Of Defense-Related Genes Responsive To Tilletia Indica Infection In Wheat, Aradhika Tripathi, Rashmi Aggarwal, Anita Yadav
Differential Expression Analysis Of Defense-Related Genes Responsive To Tilletia Indica Infection In Wheat, Aradhika Tripathi, Rashmi Aggarwal, Anita Yadav
Turkish Journal of Biology
Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, …
Plant Micrornas: New Players In Functional Genomics, Vahap Eldem, Sezer Okay, Turgay Ünver
Plant Micrornas: New Players In Functional Genomics, Vahap Eldem, Sezer Okay, Turgay Ünver
Turkish Journal of Agriculture and Forestry
MicroRNAs (miRNAs) are small, endogenously expressed, and nonprotein coding RNAs that regulate gene expression via post-transcriptional inhibition and cleavage. To date, several plant miRNAs have been identified via direct cloning, high-throughput sequencing, and bioinformatics analyses. The miRNAs participate in RNA-induced gene silencing complex, and specifically repress the target gene transcripts. Thus, miRNAs regulate the expression of genes playing diverse roles in plants, such as root initiation, leaf morphology, flower development, and response to environmental stimuli. A number of miRNAs have been identified and functionally characterized in eukaryotes. In this review, we discuss the functional roles of miRNAs in plant development …
Multiple Shoot Regeneration Of Plumular Apices Of Chickpea, Muhammad Aasim, Si̇bel Day, Fereshteh Rezaei, Mortaza Hajyzadeh
Multiple Shoot Regeneration Of Plumular Apices Of Chickpea, Muhammad Aasim, Si̇bel Day, Fereshteh Rezaei, Mortaza Hajyzadeh
Turkish Journal of Agriculture and Forestry
Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies. It is a highly recalcitrant plant and there is an urgent need to develop a regeneration protocol that can ensure easy multiple and qualitative superior shoots that could be rooted and yield fertile plants. Unconditioned plumular apices and plumular apices preconditioned with 10 mg L^{-1} benzylaminopurine (BA) for 10 days were cultured on Murashige and Skoog (MS) medium supplemented with 0.25-2.00 mg L^{-1} BA with …
Micropropagation Of Vaccinium Arctostaphylos L. Via Lateral-Bud Culture, Mustafa Cüce, Ersan Bektaş, Atalay Sökmen
Micropropagation Of Vaccinium Arctostaphylos L. Via Lateral-Bud Culture, Mustafa Cüce, Ersan Bektaş, Atalay Sökmen
Turkish Journal of Agriculture and Forestry
The aim of this study was to determine the most suitable growth medium for rapid and efficient micropropagation of Vaccinium arctostaphylos L. (Ericaceae) via lateral-bud cultures. According to preliminary studies, the best explant collection time was late April and May. Accordingly, cuttings were taken from naturally growing V. arctostaphylos populations, and lateral shoot buds were used as explant sources. Anderson's rhododendron medium (AN), McCown's woody plant medium (WPM), and Murashige and Skoog (MS) basal medium, each supplemented with zeatin/indole-3-butyric acid (IBA) (1.0/0.1 mg L^{-1}), were tested to determine the best basal medium for multiple shoot formation; WPM was the most …
Changes In Mycorrhizal Spore And Root Colonization Of Coastal Dune Vegetation Of The Seyhan Delta In The Postcultivation Phase, Özlem Aytok, Kemal Tulühan Yilmaz, İbrahi̇m Ortaş, Hali̇l Çakan
Changes In Mycorrhizal Spore And Root Colonization Of Coastal Dune Vegetation Of The Seyhan Delta In The Postcultivation Phase, Özlem Aytok, Kemal Tulühan Yilmaz, İbrahi̇m Ortaş, Hali̇l Çakan
Turkish Journal of Agriculture and Forestry
Changes in the abundance of arbuscular mycorrhizal (AM) spores and root colonization that occurred in the postcultivation phase were investigated in the coastal dunes of the Seyhan Delta, Turkey. A sampling method was used to obtain community-level information, which is essential for the evaluation of relations between plant communities and AM colonization. Eight quadrats of 10 × 10 m were selected to describe floristic composition of different plant communities. TWINSPAN was applied to identify the plant communities. In addition, DECORANA was employed to put forward a clear ordination of the communities. Soil samples were collected at depths of 0-20 cm …
Ecology, Toxicity, And Hydrolytic Activities Of Bacillus Thuringiensis In Forests, Fouad Al-Momani, Maher Obeidat
Ecology, Toxicity, And Hydrolytic Activities Of Bacillus Thuringiensis In Forests, Fouad Al-Momani, Maher Obeidat
Turkish Journal of Agriculture and Forestry
The investigation of Bacillus thuringiensis (Bt) in 16 forest soil samples from Ajloun, northern Jordan, involved the isolation of 23 isolates toxic to the third instar dipteran larvae of Drosophila melanogaster and 7 isolates toxic to the third instar lepidopteran larvae of Ephestia kuehniella. The highest viable count of Bt was found in Ebeen forest soils (14.24 × 10^7 CFU g^{-1}), and the lowest viable count was found in Rasoun forest soils. The lethal concentration (LC_{50}) of Bt isolates indicated a variation in their toxicity to D. melanogaster and E. kuehniella larvae, with lower LC_{50} values for D. melanogaster suggesting …