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Molecular Identification And Functional Characterization Of A Stilbene Synthase Gene In Rheum Officinale, Gaoxin Hao, Mengmeng Liu, Qiao Du, Xia Zhao, Feng Yan, Xiaochen Hu, Jing Gao, Liang Peng, Yimin Li, Gang Zhang Jan 2025

Molecular Identification And Functional Characterization Of A Stilbene Synthase Gene In Rheum Officinale, Gaoxin Hao, Mengmeng Liu, Qiao Du, Xia Zhao, Feng Yan, Xiaochen Hu, Jing Gao, Liang Peng, Yimin Li, Gang Zhang

Turkish Journal of Botany

Stilbene synthase (STS), is a pivotal gene involved in the biosynthesis and regulation of stilbenes compounds, playing a crucial role in plant growth, development and stress adaptation. Meantime, stilbene compounds hold promising prospects of the wide-ranging clinical applications. Rheum officinale contains abundant stilbene compounds, yet the precise regulatory genes governing their biosynthesis remain elusive. In this study, the ORF of RoSTS gene was amplified, yielding a length of 1,140 bp and encoding a protein consisting of 379 amino acids with a relative molecular mass of 41 640.25 Da. The RoSTS protein contained the conserved residues (Cys 164, His 303, and …


Effects Of Pericarp Removal And Different Germination Conditions On Seed Germination And Saline-Alkali Tolerance Of Amorpha Fruticosa, Zhan-Wu Gao, Yong-Guang Mu, Feng Chen, Li-Jie He, Xin-Ning Li, Jia-Tong Li, Ke-Jia Ding, Yan-Hui Cu, Chun-Sheng Mu, Adnan Rasheed Jan 2025

Effects Of Pericarp Removal And Different Germination Conditions On Seed Germination And Saline-Alkali Tolerance Of Amorpha Fruticosa, Zhan-Wu Gao, Yong-Guang Mu, Feng Chen, Li-Jie He, Xin-Ning Li, Jia-Tong Li, Ke-Jia Ding, Yan-Hui Cu, Chun-Sheng Mu, Adnan Rasheed

Turkish Journal of Botany

In this paper, neutral salt NaCl and basic salt Na2CO3 were used to simulate salt stress and alkali stress respectively, and the effects of salt stress and alkali stress on seed germination and growth changes in natural saline-alkali soil were studied, in order to provide theoretical basis for making full use of plant resources and improving production efficiency. The fruit peel of Amorpha fruticosa contains a lot of lipids, which has a significant effect on seed germination. Under salt stress, removal of pericarp significantly increased germination rate, germination potential and germination index (P< 0.05), and significantly shortened germination time of seeds (p< 0.05), which accelerated the establishment of seedlings, reduced the stress time during germination, and advanced the transition from heterotrophic to autotrophic, accumulating more anions and organic solutes to adapt to salt stress. Therefore, when planting in neutral salt soil, it can be used to remove the peel for sowing to improve the planting effect. However, under alkali stress, the growth is inhibited by the absorption of a large amount of Na+ and high pH value, so it is not suitable for removing the peel for sowing. Different germination conditions had significant effects on seed germination. The length and biomass of radicle and germ in sand culture were significantly higher than those in paper culture (P< 0.05). Under the condition of sand culture, the concentration of the treatment solution can be well maintained, which is conducive to fixing the seedlings and reducing the interference of other mineral elements. Therefore, sand culture method is recommended for seed germination experiments under stress, especially for plants with developed roots.


Differentiation Of Mullus Barbatus And Mullus Surmuletus (Perciformes, Mullidae) From Turkish Waters Of The Mediterranean Sea Using Otolith Shape Analyses, Semra Saygin Jan 2025

Differentiation Of Mullus Barbatus And Mullus Surmuletus (Perciformes, Mullidae) From Turkish Waters Of The Mediterranean Sea Using Otolith Shape Analyses, Semra Saygin

Turkish Journal of Zoology

Red mullet (Mullus barbatus) and surmullet (Mullus surmuletus) are valuable components of the commercial fishery industry in the Mediterranean Sea in Türkiye. To date, morphological and genetic characteristics have been used to distinguish these species. This study aimed to determine whether it was possible to distinguish between the two species using the morphometric features of their sagittal otoliths. Sagittal otoliths were subjected to elliptic Fourier analysis, wavelet analysis, and analysis of otolith shape indices for 117 specimens from the Mediterranean Sea coast of Türkiye (Mullus barbatus: 53 individuals; Mullus surmuletus: 64 individuals). Overall, canonical discriminant analysis (CDA) demonstrated strong discrimination …


Cover And Contents Jan 2025

Cover And Contents

Turkish Journal of Zoology

No abstract provided.


The Effects Of Active Substances Of Clinically Important Drugs On The Survival, Development, And Some Adult Traits Of Drosophila Melanogaster Meigen (Diptera: Drosophilidae), Gökçe Üstündağ, Kemal Büyükgüzel, Ender Büyükgüzel Jan 2025

The Effects Of Active Substances Of Clinically Important Drugs On The Survival, Development, And Some Adult Traits Of Drosophila Melanogaster Meigen (Diptera: Drosophilidae), Gökçe Üstündağ, Kemal Büyükgüzel, Ender Büyükgüzel

Turkish Journal of Zoology

Drosophila melanogaster Meigen (Diptera: Drosophilidae) was used as a model organism and fed on an artificial diet with different clinically important drugs under laboratory conditions. The effects of fluconazole, acyclovir, and piperazine on the survivorship, development, adult longevity, fecundity, and hatching rate of the insect were investigated. The results showed that the highest concentration of fluconazole (1250 mg/L) significantly decreased both the rate of larvae reaching the 3rd instar and the rate of pupation from 93.00 ± 2.95% to 11.00 ± 2.59% (χ2 = 134.696; df = 1; p = 0.0001). The highest concentration of acyclovir (200 mg/L) significantly reduced …


A Study Of The Dormant Period Of Thaumetopoea Pityocampa (Lepidoptera: Notodontidae) In Bulgaria, Gergana Zaeмdzhikova, Md Habibur Rahman Salman Jan 2025

A Study Of The Dormant Period Of Thaumetopoea Pityocampa (Lepidoptera: Notodontidae) In Bulgaria, Gergana Zaeмdzhikova, Md Habibur Rahman Salman

Turkish Journal of Zoology

The pine processionary moth (PPM), Thaumetopoea pityocampa (Denis et Schiffermuller), is a significant pest affecting conifer forests in the Mediterranean region. This study investigated the diapause mechanisms and survival strategies of PPM in summer and winter feeding populations in Bulgaria. The research was conducted from February to April 2021 at five pine plantation sites known for T. pityocampa activity in southwest Bulgaria. Herein, 1224 specimens were collected across different developmental stages. The sampled populations included those exhibiting typical winter feeding as well as atypical summer feeding. Morphological assessments and monitoring of the development of the individuals were conducted under controlled …


Testing And Optimizing Methodologies For Microplastic Extractions From Owl Pellets, Merve Seyfe, Meli̇ke Seyfe, Tolga Kankiliç, Ülkü Ni̇han Tavşanoğlu Jan 2025

Testing And Optimizing Methodologies For Microplastic Extractions From Owl Pellets, Merve Seyfe, Meli̇ke Seyfe, Tolga Kankiliç, Ülkü Ni̇han Tavşanoğlu

Turkish Journal of Zoology

Microplastic pollution is a pressing global environmental concern, potentially threatening ecosystems and entering the food chain. Owls, as nocturnal carnivorous birds, are at significant risk of ingesting microplastics due to their diverse foraging habits. This study aimed to identify the most suitable procedure for extracting microplastics from owl pellets, focusing on efficiency, functionality, and cost and ensuring the extraction of both prey and microplastics without damage. Experiments were conducted on pellets from long-eared owl (Asio otus) collected from three different habitats in Ankara, Türkiye. The extraction of microplastics was then tested using various treatments (n = 25), involving different durations, …


Effects Of A Diet Supplemented With A Blend Of Protected Organic Acids And Essential Oilson Growth Performance And Cecal Microbiota Composition In Broilers, Mehmet Akan, Kadi̇r Serdar Di̇ker, Serdar Özlü, Murat Çelebi̇, Seyyi̇de Sariçam İnce, Barişhan Doğan, Okan Eli̇bol Jan 2025

Effects Of A Diet Supplemented With A Blend Of Protected Organic Acids And Essential Oilson Growth Performance And Cecal Microbiota Composition In Broilers, Mehmet Akan, Kadi̇r Serdar Di̇ker, Serdar Özlü, Murat Çelebi̇, Seyyi̇de Sariçam İnce, Barişhan Doğan, Okan Eli̇bol

Turkish Journal of Veterinary & Animal Sciences

Organic acids (OAs) and essential oils (EOs) have several positive effects on the gut microbiota of broilers. In this study, weaimed to determine the efficacy of a blend of protected OAs and EOs [P(OA+EO)] on the growth performance and cecal microbiota ofbroiler chickens. A total of 160 Ross 308 broiler chickens, including 80 male and 80 female birds, all 1 day old, were randomly distributedbetween the control group (basal diet) and the treatment group [basal diet + 300 g/t P(OA+EO)] according to an 8-pen arrangementwith 4 replicates of 20 birds. Body weight (BW), body weight gain (BWG), feed consumption (FC), …


Organ-On-A-Chip Platforms For Drug Development, Cellular Toxicity Assessment, And Disease Modelling, Muhammad Maaz Khurram, Göktürk Ci̇nel, Özlem Yeşi̇l Çeli̇ktaş, Erdal Bedi̇r Dec 2024

Organ-On-A-Chip Platforms For Drug Development, Cellular Toxicity Assessment, And Disease Modelling, Muhammad Maaz Khurram, Göktürk Ci̇nel, Özlem Yeşi̇l Çeli̇ktaş, Erdal Bedi̇r

Turkish Journal of Biology

Organ-on-a-chips (OoCs) or microphysiological platforms are biomimetic systems engineered to mimic organ structures onto microfluidic devices for biomedical research. These microdevices can mimic biological environments that enable cell-cell interactions at a small scale by mimicking 3D in vivo microenvironments outside the body. Thus far, numerous single and multiple OoCs that recapitulate organs have been developed, and they have emerged as forerunners for drug efficacy and cytotoxicity testing. This review explores OoC platforms to portray their versatility in investigating drug safety, efficacy, and toxicity. We also reflect upon the potential of OoCs to effectively demonstrate disease models for probable novel therapeutics, …


Mechanistic Insights Into Cardiac Regeneration And Protection Through Meis Inhibition, Aynura Mammadova, Gali̇p Servet Aslan, Li Mo, Qifang Wu, Sebastian Clauss, Fati̇h Kocabaş Dec 2024

Mechanistic Insights Into Cardiac Regeneration And Protection Through Meis Inhibition, Aynura Mammadova, Gali̇p Servet Aslan, Li Mo, Qifang Wu, Sebastian Clauss, Fati̇h Kocabaş

Turkish Journal of Biology

MEIS1, a member of the TALE-type homeobox gene family, has emerged as a pivotal regulator of cardiomyocyte cell cycle arrest and represents a promising therapeutic target. Our study reveals that inhibition of MEIS1 using two novel small molecules, MEISi-1 and MEISi-2, significantly enhances neonatal cardiomyocyte proliferation and cytokinesis. Specifically, MEISi-1 and MEISi-2 increased the proportion of proliferating cardiomyocytes (Ph3+TnnT cells) upto 4.5 fold and the percentage of cytokinetic cardiomyocytes (AuroraB+TnnT cells) by 2 fold, compared to untreated controls. MEIS1 inhibition resulted in a notable downregulation of MEIS1 target genes and cyclin-dependent kinase inhibitors, demonstrating its effect on key regulatory pathways. …


Chelidonic Acid Abrogates Oxidative Stress And Memory Dysfunction In Experimental Aging Rats, Feri̇de Ni̇hal Si̇nan, Emel Serdaroğlu Kaşikçi, Burcu Çevreli̇ Dec 2024

Chelidonic Acid Abrogates Oxidative Stress And Memory Dysfunction In Experimental Aging Rats, Feri̇de Ni̇hal Si̇nan, Emel Serdaroğlu Kaşikçi, Burcu Çevreli̇

Turkish Journal of Biology

Background/aim: In an aging model established in male Wistar albino rats via the administration of D-galactose (D-gal), the aim of this study was to examine the effects of chelidonic acid on cognitive functioning and the levels of glutathione (GSH), malondialdehyde (MDA), Total antioxidant status (TAS), and brain-derived neurotrophic factor (BDNF).Materials and methods: Thirty-two Wistar albino (three month old) male rats (n = 8) were used. Group 1: Control (C), Group 2: Chelidonic acid (CA) (2 mg/kg oral gavage), Group 3: D-galactose (D-gal) (150 mg/kg, s.c.), Group 4: D-gal (150 mg/kg) + CA (2 mg/kg) (DC). Following an overnight fast, the …


Characterization Of Tfiie-Regulated Genes By Transcriptome Analysis, Serdar Baysal Dec 2024

Characterization Of Tfiie-Regulated Genes By Transcriptome Analysis, Serdar Baysal

Turkish Journal of Biology

Previous studies on GTF2E showed that it has association with certain group of diseases such as colon cancer and trichothiodystrophy but what is the global effect of GTF2E on cellular processes is not widely characterized. In this regard, this study aimed to investigate and characterize the affect of GTF2E on transcription level of genes and identify the cellular processes and diseases associated with GTF2E. The HCT116 cell line was used in the study and transfected at 30 nM concentration with siGTF2E1 or non-target negative siRNA. After 72 hours, cells were harvested and prepared for further analysis. Whole transcriptome analysis was …


Cover And Contents Dec 2024

Cover And Contents

Turkish Journal of Biology

No abstract provided.


The Cumulative Effect Of Ellagic Acid And Carnosic Acid Attenuates Oxidative Events During Diabetic Wound Healing: In Different Applications And On Different Days, Eli̇f Naz Gürsoy, Kanuni̇ Barbaros Balabanli, Fatma Nur Tuğcu Demi̇röz, Şule Coşkun Cevher Dec 2024

The Cumulative Effect Of Ellagic Acid And Carnosic Acid Attenuates Oxidative Events During Diabetic Wound Healing: In Different Applications And On Different Days, Eli̇f Naz Gürsoy, Kanuni̇ Barbaros Balabanli, Fatma Nur Tuğcu Demi̇röz, Şule Coşkun Cevher

Turkish Journal of Biology

Background/aim: The hyperglycemic environment in diabetes disrupts normal wound-healing processes, leading to chronic wounds. This study investigated whether the combination of the phenolic compounds Ellagic acid and Carnosic acid shows synergistic effects on diabetic wound healing and oxidative parameters in diabetic rats.Materials and methods: In this study, diabetic rats were divided into control, untreated, Carbopol 974P treated, topical treatment, and oral gavage treatment. Ellagic acid and Carnosic acid combination were applied topically and oral gavage to the full-thickness excisional wounds of all animals except the control group. We investigated oxidative events by malondialdehyde, glutathione, nitric oxide, protein carbonyl, collagen spectrophotometrically, …


Cannabinoid Receptor Ligands Modulate Fibrosis And Inflammation In Idiopathicpulmonary Fibrosis: A Preliminary Study, Sevi̇l Köse, Seli̇n Önen, Merve Gi̇zer, Esi̇n Boduroğlu, Uğur Gönüllü, Petek Korkusuz Dec 2024

Cannabinoid Receptor Ligands Modulate Fibrosis And Inflammation In Idiopathicpulmonary Fibrosis: A Preliminary Study, Sevi̇l Köse, Seli̇n Önen, Merve Gi̇zer, Esi̇n Boduroğlu, Uğur Gönüllü, Petek Korkusuz

Turkish Journal of Biology

Background/aim: There is no specific pharmacological treatment regimen for idiopathic pulmonary fibrosis (IPF). Therefore, new anti-inflammatory therapeutic strategies are needed. Cannabinoids (CBs) that are known for their inflammation-modulating and anti-fibrotic effects may be potential medication candidates for the treatment of IPF. Our aim is to evaluate the inflammation-modulating and anti-fibrotic effects of CB receptor (CBR) agonists and antagonists in lipopolysaccharide (LPS) stimulated human lung healthy fibroblast, epithelial cells, IPF fibroblast cells and monocytes.Materials and methods: We detected CBRs in the human lung healthy (LL24) and IPF fibroblast cells (LL29), epithelial cells (A549) and monocytes (THP-1) by flow cytometry (FC). We …


Development Of An Immunodiagnostic Assay For The Detection Of Sugarcane Mosaic Virus, Hafiz Muhammad Khalid, Najam Us Sahar Sadaf Zaidi, Naeem Rashid, Muhammad Tahir Dec 2024

Development Of An Immunodiagnostic Assay For The Detection Of Sugarcane Mosaic Virus, Hafiz Muhammad Khalid, Najam Us Sahar Sadaf Zaidi, Naeem Rashid, Muhammad Tahir

Turkish Journal of Biology

Background/Aim: Sugarcane mosaic virus (SCMV; genus Potyvirus and family Potyviridae), poses a significant threat to global sugarcane cultivars, including those in Pakistan. The aim of this study was to develop a rapid and effective diagnostic tool for detection of SCMV, enabling timely implementation of control measures to mitigate potential yield losses.Materials and Methods: The study focused on the in silico analysis; physicochemical properties, immunogenicity and subcellular localization of the SCMV coat protein (CP). The SCMV coat protein (CP) gene was synthesized, cloned and expressed in Escherichia coli. The recombinant fusion coat protein (rFCP-SCMV) was purified and used to generate polyclonal …


Combinational Therapy Of All-Trans Retinoic Acid (Atra) And Sphingomyelin Induces Apoptosis And Cell Cycle Arrest In B16f10 Melanoma Cancer Cell, Zeynep İşlek Köklü, Eli̇f Li̇dya Şanverdi̇, Başak Karadağ, Mehmet Hi̇kmet Üçişik, Ezgi̇ Taşkan, Fi̇kretti̇n Şahi̇n Dec 2024

Combinational Therapy Of All-Trans Retinoic Acid (Atra) And Sphingomyelin Induces Apoptosis And Cell Cycle Arrest In B16f10 Melanoma Cancer Cell, Zeynep İşlek Köklü, Eli̇f Li̇dya Şanverdi̇, Başak Karadağ, Mehmet Hi̇kmet Üçişik, Ezgi̇ Taşkan, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Background: Melanoma arises from the uncontrolled multiplication of melanocytes, posing an escalating global health concern. Despite early detection and surgical removal being crucial, metastatic melanoma poses treatment challenges, with limited options like chemotherapy and immunotherapy. All-trans retinoic acid (ATRA), a natural metabolite of vitamin A, has shown promise in melanoma treatment by inducing differentiation, apoptosis, growth arrest, and immune modulation. However, ATRA treatment alone can lead to resistance and relapse. Sphingomyelin (SM) also plays a role in inhibiting melanoma progression by apoptotic cell death.Objective: This study investigated combinational anticancer effects of ATRA and SM on in vitro B16F10 melanoma cells.Method: …


Synthesis, Characterization, And Investigation Of Photochemical And In Vitro Properties Of Novel Zn(Ii) Phthalocyanine, Yasemi̇n Bayğu Yildiz, Ni̇lgün Kabay, Burak Yildiz, İpek Ömeroğlu, Mahmut Durmuş, E. Riza Karagür, Hakan Akça, Çağri Ergi̇n, Yaşar Gök Dec 2024

Synthesis, Characterization, And Investigation Of Photochemical And In Vitro Properties Of Novel Zn(Ii) Phthalocyanine, Yasemi̇n Bayğu Yildiz, Ni̇lgün Kabay, Burak Yildiz, İpek Ömeroğlu, Mahmut Durmuş, E. Riza Karagür, Hakan Akça, Çağri Ergi̇n, Yaşar Gök

Turkish Journal of Chemistry

In our work, a new non-peripheral zinc(II) phthalocyanine bearing octa carboxylic acid ethyl ester derivative substituted triazole attached propylmercaptothiobenzylmercapto derivative was synthesized by the tetramerization reaction of phthalonitrile. The photochemical in vitro photodynamic activity of zinc(II) phthalocyanine (ZnPc-I), such as human non-small cell lung carcinoma cell lines, was investigated in this study. The singlet oxygen generation property of novel zinc(II) phthalocyanine (ZnPc-I) was also examined due to the significantly high singlet oxygen quantum yield of ZnPc-I (ΦΔ = 0.66). The antiproliferative effects of ZnPc-I were also investigated on A549 and H1299 cell lines, our results demonstrate that the ZnPc-I has …


Understanding Ammonia's Role In Mitigating Concentration Polarization In Anion-Exchange Membrane Electrodialysis, Abdallah Timmaoui, Mahmoud Ferhat, Nesri̇ne Souad Ferhat, Ahmed Hamdi Dec 2024

Understanding Ammonia's Role In Mitigating Concentration Polarization In Anion-Exchange Membrane Electrodialysis, Abdallah Timmaoui, Mahmoud Ferhat, Nesri̇ne Souad Ferhat, Ahmed Hamdi

Turkish Journal of Chemistry

In processes such as electrodialysis, the applied electrical potential is constrained by concentration polarization at the membrane/solution interface. This polarization, which intensifies at higher current densities, impedes ion transport efficiency and may lead to problems such as salt precipitation, membrane degradation, and increased energy consumption. Therefore, understanding concentration polarization is essential for enhancing membrane performance, improving efficiency, and reducing operational costs. This study investigates the impact of ammonia buffer (NH₄⁺/NH₃) on sulfate ion transport through anion-exchange membranes, with a particular focus on limiting current density and concentration polarization under constant current conditions. Our findings demonstrate that ammonia effectively eliminates concentration …


New Naphthalene-Linked Pyrazoline-Thiazole Hybrids As Prominent Antilung And Antibreast Cancer Inhibitors, Hali̇li̇brahi̇m Çi̇ftçi̇, Masami Otsuka, Mikako Fujita, Belgi̇n Sever Dec 2024

New Naphthalene-Linked Pyrazoline-Thiazole Hybrids As Prominent Antilung And Antibreast Cancer Inhibitors, Hali̇li̇brahi̇m Çi̇ftçi̇, Masami Otsuka, Mikako Fujita, Belgi̇n Sever

Turkish Journal of Chemistry

Epidermal growth factor receptor (EGFR) and HER2, pioneer members of the receptor tyrosine kinase subfamily, are frequently mutated and/or overexpressed in several types of human cancers, including non-small cell lung cancer (NSCLC) and breast cancer, which are leading causes of cancer-related deaths worldwide. EGFR and HER2-focused anti-NSCLC and anti-breast cancer studies encouraged us to search for new potential agents. For this purpose, in the current work, naphthalene-linked pyrazoline-thiazole hybrids (BTT1-10 and BTP1-10) were synthesized and examined for their antiproliferative effects on A549 NSCLC and MCF-7 breast cancer cell lines. According to the results, MTT assay showed that BTT-5 induced strong …


Preparation Of Manganese-Doped Carbon Quantum Dots@Halloysite Nanotubes Composites Films For Advanced Uv Shielding Properties, Xiao Zhang, Bin Wang Dec 2024

Preparation Of Manganese-Doped Carbon Quantum Dots@Halloysite Nanotubes Composites Films For Advanced Uv Shielding Properties, Xiao Zhang, Bin Wang

Turkish Journal of Chemistry

The development of Ultraviolet (UV) shielding materials are of great importance to protect human's health concerns and prevent the degradation of organic matters. However, the synthesis of highly efficient UV shielding polymer nanocomposites is currently limited by the agglomeration of inorganic anti-UV nanoparticles (NPs) within the polymer matrix and limited absorption spectrum of UV shielding agents. In this study, highly effective manganese doped carbon quantum dots@halloysite nanotubes composites (Mn-CDs@HNTs/PAS) were successfully synthesized by loading manganese doped carbon quantum dot (Mn-CDs) into UV shielding effective halloysite nanotubes (HNTs) via solvothermal method, followed by polymerization modification. The results show that inorganic NPs …


Synthesis, Structure, Hirshfeld Surface Analysis, And Molecular Docking Studies Of The Cocrystal Between The Cu(Ii) Complex Of Salicylic Acid And Uncoordinated Piracetam, Nazokat N. Yuldasheva, Ikram I. Abdullaev, Oybek I. Khudoyberganov, Lola A. Gandjaeva, Pirnazar K. Kodamboev, Elyor Sh. Samandarov, Adkhamjon S. Normamatov, Abror Kh. Ruzmetov, Yuldosh Y. Yakubov, C. Balakrishnan, Bakhtiyar T. Ibragimov, Aziz Bakhti̇yarovich Ibragimov Dec 2024

Synthesis, Structure, Hirshfeld Surface Analysis, And Molecular Docking Studies Of The Cocrystal Between The Cu(Ii) Complex Of Salicylic Acid And Uncoordinated Piracetam, Nazokat N. Yuldasheva, Ikram I. Abdullaev, Oybek I. Khudoyberganov, Lola A. Gandjaeva, Pirnazar K. Kodamboev, Elyor Sh. Samandarov, Adkhamjon S. Normamatov, Abror Kh. Ruzmetov, Yuldosh Y. Yakubov, C. Balakrishnan, Bakhtiyar T. Ibragimov, Aziz Bakhti̇yarovich Ibragimov

Turkish Journal of Chemistry

The cocrystal or supramolecular complex between Cu(II) complex of salicylic acid and uncoordinated piracetam has been synthesized. Its structure is characterized by element analysis, FT-IR, UV-Visible spectroscopy and X-ray crystallography. Spectroscopic methods confirm the formation of the metal complex while X-ray crystallography establishes molecular and crystal structure of the obtained compound. Binuclear Cu(II) complex of salicylic acid (complex molecule) is in the form of “Chinese lantern” and it interacts with uncoordinated piracetam by complicated system of H-bonds. However, according to the Hirshfeld surface analysis a contribution of the O•••H/H•••O contacts is only 24.9% while H•••H and H•••C/C•••H contacts account for …


A Fast And Responsive Turn-On Fluorescent Probe Based On A Quinone Conjugated Alkoxy Derivative For Biothiols And A Cellular Imaging Study, Ulviyye Nemetova, Ayşe Nur Önem, Alev Er, Sefa Çeli̇k, Ayşen E. Özel, Sevi̇m Akyüz, Mustafa Özyürek, Si̇bel Şahi̇nler Ayla Dec 2024

A Fast And Responsive Turn-On Fluorescent Probe Based On A Quinone Conjugated Alkoxy Derivative For Biothiols And A Cellular Imaging Study, Ulviyye Nemetova, Ayşe Nur Önem, Alev Er, Sefa Çeli̇k, Ayşen E. Özel, Sevi̇m Akyüz, Mustafa Özyürek, Si̇bel Şahi̇nler Ayla

Turkish Journal of Chemistry

Detection of intracellular biothiols (cysteine (CYS), N-acetyl cysteine (NAC) and glutathione (GSH)) with high-selectivity and sensitivity is important to reveal biological functions. In this study, 2-(2-methoxy-4-methylphenoxy)-3-chloro-5,8-dihydroxynaphthalene-1,4-dione (3) (DDN-O) compound was newly synthesized and used as a fluorogenic probe (detector molecule) in the fluorometric method for the rapid, highly selective and sensitive determination of biothiols. The intensity values (λex = 260 nm, λem = 620 nm) of the product were measured by adding biotihols to the reaction medium at varying concentrations and the glutathione equivalent thiol content (GETC) values of each biotiol were calculated. Using the compoud (3), the reference biotiol …


Functional Characterization Of Haloarchaeal Udp-Glucose 4-Epimerase And 3-Hydroxybutyryl-Coa Dehydrogenase Genes In Nicotiana Tabacum, Çi̇ğdem Otur, Sezer Okay, Aslihan Kurt Kizildoğan Dec 2024

Functional Characterization Of Haloarchaeal Udp-Glucose 4-Epimerase And 3-Hydroxybutyryl-Coa Dehydrogenase Genes In Nicotiana Tabacum, Çi̇ğdem Otur, Sezer Okay, Aslihan Kurt Kizildoğan

Turkish Journal of Botany

The objective of the studyAbiotic stress poses a significant threat to plant survival. In response to the challenging conditions, plants have evolved various resistance mechanisms. Recently, genetic strategies have become increasingly prevalent in efforts to enhance abiotic stress tolerance and develop stress-resistant plant varieties. In this study, we employed heterologous expression of two metabolism-related haloarchaeal genes in the Nicotiana tabacum genome to improve its mechanism for salt and drought tolerance.Experimental procedure(s)The U2642 and CL165 genes encoding 3-hydroxybutyryl-CoA dehydrogenase and UDP-glucose 4-epimerase, respectively, were subcloned into the pIPKb004 vector under the control of Cauliflower mosaic virus 35S (CaMV35S) promoter using the …


Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges, Bayram Ali̇ Yerli̇kaya, Karam Mostafa, Abdullah Aydin, Seher Yerli̇kaya, Momna Mehmood, Ni̇sa Nur Yilmaz, Kubi̇lay Yildirim, Musa Kavas Dec 2024

Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges, Bayram Ali̇ Yerli̇kaya, Karam Mostafa, Abdullah Aydin, Seher Yerli̇kaya, Momna Mehmood, Ni̇sa Nur Yilmaz, Kubi̇lay Yildirim, Musa Kavas

Turkish Journal of Botany

Developments in genome editing and plant biotechnology in recent years, especially CRISPR-Cas9 and RNA interference (RNAi) technologies, offer essential tools for solving global agricultural problems that are worsened by climate change and the increasing food demand caused by the rising world population. This review focuses on the urgent need to feed a growing global population. It explores how key biotechnological tools can enhance crop resilience and nutritional quality amid climate variability and extreme weather. The applicability of CRISPR-Cas technologies in the development of climate-friendly, more nutritious, and higher-yielding crops has opened a biotechnological avenue for the development of both transgenic …


Advancements In Qtl Mapping And Gwas Application In Plant Improvement, Muhammad Tanveer Altaf, Muhammed Tatar, Amjad Ali, Waqas Liaqat, Parnaz Mortazvi, Ceyhun Kayihan, Fati̇h Ölmez, Muhammad Azhar Nadeem, Jazib Javed, Jin-Ying Gou, Meng-Lu Wang, Ummad Ud Din Umar, Hayri̇ye Yildiz Daşgan, Cemal Kurt, Mehtap Yildiz, Sheikh Mansoor, Abdelfattah A. Dababat, Nafiz Çeli̇ktaş, Faheem Shehzad Baloch Dec 2024

Advancements In Qtl Mapping And Gwas Application In Plant Improvement, Muhammad Tanveer Altaf, Muhammed Tatar, Amjad Ali, Waqas Liaqat, Parnaz Mortazvi, Ceyhun Kayihan, Fati̇h Ölmez, Muhammad Azhar Nadeem, Jazib Javed, Jin-Ying Gou, Meng-Lu Wang, Ummad Ud Din Umar, Hayri̇ye Yildiz Daşgan, Cemal Kurt, Mehtap Yildiz, Sheikh Mansoor, Abdelfattah A. Dababat, Nafiz Çeli̇ktaş, Faheem Shehzad Baloch

Turkish Journal of Botany

Molecular markers play a crucial role in modern plant breeding by enabling the precise identification of genetic loci associated with important agronomic traits. This article focuses on the advancements in Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS), which are pivotal in identifying and dissecting complex traits such as stress tolerance, yield improvement, and nutrient efficiency. Through QTL mapping, significant genetic regions linked to desired traits are identified, while GWAS leverages larger populations for higher resolution in genetic association studies. The integration of next-generation sequencing, CRISPR, and transcriptomics technologies has revolutionized the precision and efficiency of these methods, …


Identification Of Genes Associated With Zinc And Copper Concentrations In Walnut (Juglans Regia L.) By Gwas Analysis, Berfi̇n Nazlican Kaman, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş Dec 2024

Identification Of Genes Associated With Zinc And Copper Concentrations In Walnut (Juglans Regia L.) By Gwas Analysis, Berfi̇n Nazlican Kaman, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Botany

154 walnut genotypes from various geographic regions were analyzed in the study. Micronutrient concentrations of zinc (Zn) ranged from 1.61 to 8.98 mg/100 g, and the concentrations of copper (Cu) varied between 0.12 and 0.38 mg/100 g. 16,473 SNPs were generated using Diversity Array Technology (DArT) for genome-wide association (GWAS) studies. The Mixed Linear Model (MLM) utilizing the Q + K model revealed seven associated markers for the element Zn in 2021 and four in 2022. The MLM (Q + K) model indicated two associated markers for the element Cu in 2021 and three in 2022. The markers found in …


Gene Expression Changes In The Seagrass Cymodocea Nodosa Individuals In Response To Aquatic Acidification, Enes Göksal, Esra Öztürk Yi̇ği̇t Dec 2024

Gene Expression Changes In The Seagrass Cymodocea Nodosa Individuals In Response To Aquatic Acidification, Enes Göksal, Esra Öztürk Yi̇ği̇t

Turkish Journal of Botany

Human activities have caused a rise in atmospheric carbon dioxide (CO2) levels, leading to greater absorption of CO2 by oceans and causing ocean acidification. This phenomenon, marked by a reduction in pH, represents substantial risks to marine ecosystems, including seagrass meadows. Seagrasses are vital elements of coastal ecosystems, performing important functions in carbon storage, stabilizing shorelines, and preserving biodiversity; however, reactions to ocean acidification are not well understood, especially in terms of molecular.This research study examined alterations in gene expression within seagrass meadows, namely the species Cymodocea nodosa, in reaction to simulated ocean acidification conditions. Climate chamber system to adjust …


Cover And Contents Dec 2024

Cover And Contents

Turkish Journal of Botany

No abstract provided.


Selective Indel Marker Identification Across The Peanut (Arachis Hypogaea L.) Genome Using Ddradseq, Moin Qureshi, Bi̇rgül Guden, Hi̇lal Şule Tosun, Mojtaba Kordrostami, Sunil Shiwaji Gangurde, Bülent Uzun, Engi̇n Yol Dec 2024

Selective Indel Marker Identification Across The Peanut (Arachis Hypogaea L.) Genome Using Ddradseq, Moin Qureshi, Bi̇rgül Guden, Hi̇lal Şule Tosun, Mojtaba Kordrostami, Sunil Shiwaji Gangurde, Bülent Uzun, Engi̇n Yol

Turkish Journal of Botany

The advent of next-generation sequencing technologies, mainly double digest restriction site-associated DNA sequencing (ddRADSeq), has significantly advanced the development of molecular markers for crop genetics. This study used ddRADSeq to identify and develop insertion-deletion (InDel) markers in 25 peanut genotypes from diverse geographic regions. The bioinformatic analysis unveiled 62728 InDels across the peanut genome, predominantly between 1-5 bp, which constituted 96% of the total, while InDels of ≥ 6 bp accounted for 3.96%. We focused on 1013 InDels of at least 10 bp for further analysis, representing 1.61% of the total reads, with a distribution of 832 insertions and 181 …