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Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Turkish Journal of Biology
The yeast Pichia pastorisexpression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZ\alphaA expression vector under the control of the methanol-inducible alcohol oxidase (AOX1) promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the alpha-factor mating signal sequence of the expression vector. Analysis of the …
Efficacy Of Trichogramma Evanescens And Bacillus Thuringiensis Var. Kurstaki In Control Of Cydia Pomonella (L.) In Turkey, Sevcan Öztemi̇z, Ali̇ Küden, Serpi̇l Nas, Işilay Lavkor
Efficacy Of Trichogramma Evanescens And Bacillus Thuringiensis Var. Kurstaki In Control Of Cydia Pomonella (L.) In Turkey, Sevcan Öztemi̇z, Ali̇ Küden, Serpi̇l Nas, Işilay Lavkor
Turkish Journal of Agriculture and Forestry
This study was conducted to evaluate the effectiveness of Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae) release with as well as without Bacillus thuringiensis var. kurstaki (Berliner) in biological control of Cydia pomonella (L.) (Lepidoptera: Tortricidae) in the Galaxy Gala apple variety grafted onto M9 rootstock in 2016. Four treatments were analyzed: releasing T. evanescens alone (TE), applying B. thuringiensis var. kurstaki (BT) alone, applying both (TE + BT), and a control (C) without any application at all. The experiment design entailed randomized blocks with four replicates. In each generation of the egg stage, 100,000 parasitoids per hectare were released twice (1440 …
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Turkish Journal of Biology
Mitochondrial dysfunction has been previously identified in neurodegenerative diseases such as Alzheimer disease, Huntington disease, and Parkinson disease. Chemical inhibition of the mitochondrial electron transport chain (ETC) was shown to trigger symptoms in animal models similar to those observed in human neurodegenerative diseases. In order to understand the effect of mitochondrial dysfunction on the proteome level, LC-MSE-based bottom-up, label-free differential proteomics expression analysis was used to monitor protein level changes in SH-SY5Y neuroblastoma cells induced by ETC-specific inhibitors (MPTP, 3-NP, sodium azide, antimycin A, and oligomycin). A total of 379 proteins were identified across the sample set and 75 of …
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Turkish Journal of Biology
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were …
Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary
Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary
Turkish Journal of Biology
The Sargassum species are prospective candidates for marine culture, but there are a limited number of reports on their nutrient requirements and optimum initial stocking biomass, and nothing is published for Sargassum spinuligerum. This study investigated the effects of three commercially available fertilizers (Hortico, Seasol, and Aquasol) and four initial stocking biomass levels of S. spinuligerum on the growth rate and nutrient uptake capacities for 7 weeks. The results showed that S. spinuligerum could be grown under outdoor conditions with the optimum initial stocking biomass of 15.35 g per 113 L. The different commercial fertilizers significantly influenced the specific growth …
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Turkish Journal of Biology
Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …
Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu
Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Biology
Human carbonic anhydrase II (hCA II) enzyme was firstly expressed using a pET-SUMO expression vector in Escherichia coli and the recombinant enzyme was purified using nickel (Ni2+) affinity chromatography. The substitutions of Trp 209 with four amino acid (Val, Leu, Ile, and Pro) in the hydrophobic pocket of hCA II were conducted using site-directed mutagenesis. The p-nitrophenyl esterase activity of hCA II variants correlates with the hydrophobicity and size of residue, suggesting that the hydrophobic character of this residue is important for catalysis. The Trp 209 was forecast as an important residue and was exposed to computational mutagenesis. This forecast …
Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic
Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic
Turkish Journal of Biology
Galectins (gals) are ß-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin …
Natural Dissemination Of Hypovirulent Cryphonectria Parasiticastrain Used For Biological Control Of Chestnut Blight, Naci̇ye Mükerrem Çeli̇ker, Cevdet Kaplan, Emi̇n Ersi̇n Onoğur, Barbaros Çeti̇nel, Di̇lek Poyraz, Ayşe Uysal
Natural Dissemination Of Hypovirulent Cryphonectria Parasiticastrain Used For Biological Control Of Chestnut Blight, Naci̇ye Mükerrem Çeli̇ker, Cevdet Kaplan, Emi̇n Ersi̇n Onoğur, Barbaros Çeti̇nel, Di̇lek Poyraz, Ayşe Uysal
Turkish Journal of Agriculture and Forestry
Chestnut blight, caused by Cryphonectria parasitica Murr. Barr, is the most serious disease of chestnut trees in Turkey on which several studies related to biological control were conducted. The aim of this study was to determine the cause of the occurrence of healing cankers on untreated chestnut trees and the role of carriers in the spread of hypovirulence by laboratory tests, and to discover the effect of some environmental factors (temperature and relative humidity) on the natural spreading of hypovirulence. The study was carried out in a chestnut grove where biological control studies of the disease had been performed in …
In Vitro Production Protocol Of Vaccinium Uliginosum L. (Bog Bilberry)Growing In The Turkish Flora, Mustafa Cüce, Atalay Sökmen
In Vitro Production Protocol Of Vaccinium Uliginosum L. (Bog Bilberry)Growing In The Turkish Flora, Mustafa Cüce, Atalay Sökmen
Turkish Journal of Agriculture and Forestry
This novel approach was designed to increase the production capacity of Vaccinium uliginosum L. via direct organogenesis. Lateral buds containing one or two leaves were initially cultured in McCown woody plant medium (WPM), Anderson's rhododendron medium, and Murashige and Skoog basal media, each supplemented with zeatin/indole-3-butyric acid (IBA) and zeatin/naphthalene acetic acid (NAA) (1.0/0.1 mg L-1). WPM containing the zeatin/IBA combination was the most effective as the basal medium. Various plant growth regulators (PGRs) were then investigated for the best shoot multiplication, and zeatin was found to be most favorable PGR in all cases. The highest shoot length and shoot …
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Turkish Journal of Biology
In the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down- and upregulation of the miRNAs were compared using the ΔΔCT method. At the last stage of the study, we …
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Lateral flow assay (LFA), or the immunochromatographic strip test, is popular to use for rapid and sensitive immunoassays. Gold nanoparticles (GNPs), due to tunable optical characteristics and easy manipulation of size or shape, represent an attractive approach for LFA technology. Since most enterohemorrhagic infections result from water and food contaminations of Escherichia coli O157:H7, selective and rapid detection of this organism in environmental and biological complexes is necessary. In this study, optimized parameters of antibody (Ab)-based LFA for rapid detection of pathogenic E. coli O157:H7 are described. GNPs were used as visualizing agents. The measuring parameters include the Ab concentration …
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Turkish Journal of Biology
The characteristics of adipose-derived mesenchymal stem cells (Ad-MSCs) can be selectively enhanced by altering the culture environment. We evaluated the effects of gelatin on Ad-MSCs when used in combination with culture media. Ad-MSCs were initially cultured in 0%, 0.5%, 1%, 2%, and 4% gelatin in Dulbecco's modified Eagle's medium (DMEM) to evaluate cell proliferation. The expression of inflammatory, antiinflammatory, antioxidant, and osteogenic markers was then assessed by rtPCR in Ad-MSCs cultured in 0.5% gelatin in DMEM (GMSCs), and without gelatin (MSCs), for 5 and 10 days. We found that 0.5% gelatin significantly increased the proliferation rate of Ad-MSCs after 24 …
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Turkish Journal of Biology
For long-term treatment of diabetes type 1, transplantation of insulin-producing beta cells may be a promising method, but the limited number of islets for transplantation requires the development of different approaches. In this study, we aimed to generate beta-like insulin-producing cells. For this purpose, MafA, Pax4, and Ngn3 genes were transferred into pancreatic islet-derived mesenchymal stem cells, and the effect of their ectopic expressions on differentiation efficiency was examined. Stemness properties of pancreatic islet stem cells were characterized. The 3 genes were transfected by electroporation and expressed constitutively. The transfected cells were further stimulated to differentiate by using chemical induction. …
Influence Of Vermicompost Application On Soil Consistency Limits And Soil Compactibility, Serdar Sari, Ekrem Lütfi̇ Aksakal, İlker Angin
Influence Of Vermicompost Application On Soil Consistency Limits And Soil Compactibility, Serdar Sari, Ekrem Lütfi̇ Aksakal, İlker Angin
Turkish Journal of Agriculture and Forestry
Soil organic matter (SOM) is a composite indicator of soil quality. Recycling of organic wastes is one of the environmentally compatible and economically viable options to increase and/or maintain SOM content. Our study was conducted to evaluate the effects of vermicompost (0%, 0.5%, 1%, 2%, and 4% w/w) on the consistency limits and the Proctor compaction test parameters of three texturally different soils (sandy loam, loam, and clay) from similar climatic conditions and cropping management practices. Results showed that vermicompost increased the optimum moisture content (OMC) with an associated decrease in maximum dry soil bulk density (MBD). The increase in …
Response Of Five Triticale Genotypes To Salt Stress In In Vitro Culture, İsmai̇l Bezi̇rğanoğlu
Response Of Five Triticale Genotypes To Salt Stress In In Vitro Culture, İsmai̇l Bezi̇rğanoğlu
Turkish Journal of Agriculture and Forestry
Salinity is one of the important abiotic stresses that restrict plant development. Triticale is a highly adaptable crop, known for its high-quality grain, yield potential, and resistance to disease. Responses of five cultivated genotypes (Ümran Hanım, Mikham 2002, Melez 2001, Tatlıcak, and Alper Bey) of triticale to salt stress were tested in callus and embryogenic callus formation in this study. Cotyledon explants were selected as explants for callus induction and embryogenic callus formation. The five tested triticale genotypes varied in their callus growth and embryogenic callus formation. Ümran Hanım, Tatlıcak, and Alper Bey were observed for better callus induction; similarly, …
Diversity And Genetic Analysis Through Dartseq In Common Bean(Phaseolus Vulgaris L.) Germplasm From Turkey, Seda Nemli̇, Tansel Kaygisiz Aşçioğul, Duygu Ateş, Dursun Eşi̇yok, Muhammed Bahatti̇n Tanyolaç
Diversity And Genetic Analysis Through Dartseq In Common Bean(Phaseolus Vulgaris L.) Germplasm From Turkey, Seda Nemli̇, Tansel Kaygisiz Aşçioğul, Duygu Ateş, Dursun Eşi̇yok, Muhammed Bahatti̇n Tanyolaç
Turkish Journal of Agriculture and Forestry
The common bean (Phaseolus vulgaris L.) is the most consumed food legume in the world and is a major source of dietary protein, carbohydrates, and valuable micronutrients, especially in developing countries. Diversity Arrays Technology (DArTseq), based on genome reduction with restriction enzymes, provides a rapid, high-throughput, and cost-effective tool capable of generating thousands of genotyped single nucleotide polymorphisms (SNPs) for a genome-wide analysis of genetic diversity. In this study, we aimed to characterize common bean accessions using SNPs detected by a DArTseq approach. A total of 43,018 SNPs were identified from 173 common bean accessions, including Andean and Mesoamerican genotypes. …
Chemical Composition And Morphometric Traits And Yield Ofcarrots Grown In Organic And Integrated Farming Systems, Natasa Sink, Maja Mikulic Petkovsek, Robert Veberic, Nina Kacjan Marsic
Chemical Composition And Morphometric Traits And Yield Ofcarrots Grown In Organic And Integrated Farming Systems, Natasa Sink, Maja Mikulic Petkovsek, Robert Veberic, Nina Kacjan Marsic
Turkish Journal of Agriculture and Forestry
Changes in chemical composition and quality traits of carrot roots (Daucus carota L.) grown in organic (ORG) and integrated (INT) farming systems were investigated during two successive years. Three carrot cultivars (Fanal, Rodelika, and Rolanka) were included. Determinations of sugars, organic acids, α- and ß-carotene, and vitamin C were performed with an HPLC system, and a liquid chromatography-mass spectrometry (LC-MS) system was used for the identification and quantification of phenolics. A higher yield of carrot in the ORG system was observed (42-44 t ha-1) as compared with the INT system (37-40 t ha-1). The impact of farming system on morphometric …
Antioxidant Capacity Of Oilseed Rape (Brassica Napus) In Different Soil Types, Dusica Jovicic, Jovica Vasin, Zorica Nikolic, Gordana Petrovic, Gordana Tamindzic, Maja Ignjatov, Dragana Milosevic
Antioxidant Capacity Of Oilseed Rape (Brassica Napus) In Different Soil Types, Dusica Jovicic, Jovica Vasin, Zorica Nikolic, Gordana Petrovic, Gordana Tamindzic, Maja Ignjatov, Dragana Milosevic
Turkish Journal of Agriculture and Forestry
This work considers the results obtained by testing the parameters of antioxidant status in leaves and roots of oilseed rape at different growth stages (phase I: formation of leaf rosettes and preparing for winter hibernation; phase II: after the winter hibernation; phase III: flowering) grown in semicontrolled field conditions on different soil types. The experiment was conducted on four oilseed rape genotypes (Banacanka, Jasna, Kata, and Zlatna) in semicontrolled conditions in containers with three soil types: chernozem, solonetz, and solonchak. The germination failed on solonchak due to the excessive amount of salt, but results were obtained for plant material on …
The Effects Of Different Plant Densities And Silage Corn Varieties On Silage Yield And Some Yield Parameters In No-Till Seeding, Mehmet Zahi̇d Malasli, Ali̇ Khatami̇, Ahmet Çeli̇k
The Effects Of Different Plant Densities And Silage Corn Varieties On Silage Yield And Some Yield Parameters In No-Till Seeding, Mehmet Zahi̇d Malasli, Ali̇ Khatami̇, Ahmet Çeli̇k
Turkish Journal of Agriculture and Forestry
In this study, the possibility of growing silage corn, which is used as an important nutrient in livestock and whose production is increasing in recent years, by no-till seeding method under Erzurum conditions was investigated. For this purpose, three silage corn varieties (DKC-5783, Prestige, and ADA-9510) suitable for Erzurum's climatic conditions in terms of vegetation period were selected from a large number of silage corn seeds widely grown throughout Turkey and their development conditions under no-till seeding conditions were investigated. A precision vacuum no-till seeder with double disk type furrow opener was used for silage corn seeding. Based on three …
Aftermath Of The Human Genome Project: An Era Of Struggle And Discovery, Zainab Hamdoun, Hashimul Ehsan
Aftermath Of The Human Genome Project: An Era Of Struggle And Discovery, Zainab Hamdoun, Hashimul Ehsan
Turkish Journal of Biology
The collaboration between the Human Genome Project and the Sanger Institute, spurred on by their contemporary- Celera Genomics, in the race to sequence the human genome, opened the door to a generation of research concerned with the study of the nucleic manual. Following this monumental achievement, the genomic era was born, heralding the development of gene therapy, personalized medicine, pharmacogenomics, and CRISPR/Cas9 gene-editing systems. These 'omic' disciplines offer a wide range of applications in the treatment of disease and the betterment of the quality of human life. Their notability, however, manifests in the unique mix of sociological and scientific discourse …
Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le
Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le
Turkish Journal of Biology
Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis. In the following investigation, a novel extracellular protein with antifungal activity against Rhizoctonia solani and Fusarium oxysporum was optimally produced, purified, and characterized from a native Serratia marcescens DT3. Under optimal conditions (medium containing 1.25% peptone, 0.5% yeast extract; temperature of 28 °C; initial pH of 7; culture time of 28 h), the antifungal activity of the …
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Turkish Journal of Biology
The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture. Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitro-raised plantlets as compared to 35 and 20 DAP. At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery …
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
Turkish Journal of Biology
Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs …
Genetic Variation And Potential Genetic Resources Of Several Romanian Larch Populations, Alina Vilcan (Truta), Lucica Mihalte (Crisan), Adriana F. Sestras, Liviu Holonec, Radu E. Sestras
Genetic Variation And Potential Genetic Resources Of Several Romanian Larch Populations, Alina Vilcan (Truta), Lucica Mihalte (Crisan), Adriana F. Sestras, Liviu Holonec, Radu E. Sestras
Turkish Journal of Agriculture and Forestry
RAPD molecular markers and phenotypic growth traits were joined using different genotypes of Larix decidua (Mill.) to analyze genetic variation. Individual samples of vegetative clones were analyzed from Baciu orchard (obtained through a breeding program from regional stands from Romania) and pooled samples from places of origin (regional stands). Based on coefficients of variation for tree height, diameter, and volume, the greatest genetic variation in the orchard was observed at the Gura Humorului and Anina provenances (24.4%, 27.5%, and 14.4% for Gura Humorului and 26.9%, 29.46%, and 15.5% for Anina). In addition, the trees whose origins are Gura Humorului and …
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
Turkish Journal of Biology
This study reports the generation of a new human muscle tissue equivalent from skeletal muscle-derived stem cells and human umbilical vein endothelial cells (HUVECs). Skeletal muscle stem cells were isolated by the preplate technique and differentiated into neuron-like cells that were positive for neuronal beta-tubulin3 and nestin and negative for the astrocyte marker glial fibrillary acidic protein (GFAP). Coculture of skeletal muscle stem cells with the HUVECs under optimized fetal bovine serum and media conditions resulted in formation of a capillary network among the multinucleated myotubes. The neuron-like cells derived from the human skeletal muscle stem cells were seeded onto …
Tree-Induced Changes In A Terra Rossa Soil Under Olive.Observations From An Integrated Field Study, Salvatore Madrau, Claudio Zucca, İhsan Akşi̇t, Zülküf Kaya, Seli̇m Kapur
Tree-Induced Changes In A Terra Rossa Soil Under Olive.Observations From An Integrated Field Study, Salvatore Madrau, Claudio Zucca, İhsan Akşi̇t, Zülküf Kaya, Seli̇m Kapur
Turkish Journal of Agriculture and Forestry
A range of indicators including chemical, physical, morphological, and clay mineral properties were investigated to support an integrated evaluation of the changes induced by a long established olive grove on a red Mediterranean soil. The study area was located in Sardinia (Italy). Two soil profiles were studied in a site where land use had remained unchanged for the previous 150 years: one under the canopy and around the trunk of an olive tree and one between the trees. Chemical analyses including organic matter fractions were performed by horizon. Undisturbed soil samples were collected from aggregates for thin section and scanning …
Recent Advances In Thymidine Phosphorylase Inhibitors: Syntheses And Prospective Medicinal Applications, Muhammad Aamir Sajid, Zulfiqar Ali Khan, Sohail Anjum Shahzad, Syed Ali̇ Raza Naqvi, Muhammad Usman, Ahsan Iqbal
Recent Advances In Thymidine Phosphorylase Inhibitors: Syntheses And Prospective Medicinal Applications, Muhammad Aamir Sajid, Zulfiqar Ali Khan, Sohail Anjum Shahzad, Syed Ali̇ Raza Naqvi, Muhammad Usman, Ahsan Iqbal
Turkish Journal of Chemistry
This review paper covers recent advances in the synthetic strategies for novel TPase inhibitors and their potential medicinal applications over the last few decades. A brief introduction covering the structural aspects of TPase inhibitors is also included to facilitate understanding of diverse approaches to monitor the design of new inhibitors. TPase is an essential enzyme and its inhibition is a potential target in the development of anticancer drugs.
Sonochemical Degradation Of Basic Red 29 In Aqueous Media, Zineb Boutamine, Slimane Merouani, Oualid Hamdaoui
Sonochemical Degradation Of Basic Red 29 In Aqueous Media, Zineb Boutamine, Slimane Merouani, Oualid Hamdaoui
Turkish Journal of Chemistry
In this work, the degradation of Basic Red 29 (BR29), an azo dye, was investigated using a high-frequency ultrasonic reactor (300 kHz). The influence of several parameters such as initial substrate concentration (5--200 mg L$^{-1})$, pH (3-10.1), ultrasonic power (20-80 W), and nature of the dissolved gas (Ar, air, and N$_{2})$ on the degradation of BR29 was assessed. Additionally, the impact of natural matrices (seawater and natural and river waters) on the degradation rate of BR29 was clarified. Degradation experiments with radical scavengers, tert-butyl alcohol and glucose, showed that BR29 degraded mainly through HO$^{\bullet }$ radical attack at the gas/liquid …
Voltammetric Analysis Of Disulfiram In Pharmaceuticals With A Cyclic Renewable Silver Amalgam Film Electrode, Sylwia Smarzewska, Natalia Festinger, Monika Skowron, Dariusz Guziejewski, Radovan Metelka, Mariola Brycht, Witold Ciesielski
Voltammetric Analysis Of Disulfiram In Pharmaceuticals With A Cyclic Renewable Silver Amalgam Film Electrode, Sylwia Smarzewska, Natalia Festinger, Monika Skowron, Dariusz Guziejewski, Radovan Metelka, Mariola Brycht, Witold Ciesielski
Turkish Journal of Chemistry
Electrochemical properties of disulfiram, representative of highly significant bioactive compounds, were studied with a cyclic renewable silver amalgam film electrode (Hg(Ag)FE) using square wave cathodic stripping voltammetry (SWCSV). The influence of various factors such as pH, buffer concentration, buffer composition, and SWCSV parameters on current response was investigated. The optimum results in terms of signal shape and intensity were recorded in Britton--Robinson buffer (pH 7.5) at -0.5 V versus Ag/AgCl/3 mol L$^{-1}$ KCl. An elaborated electroanalytical procedure was used to determine disulfiram at the Hg(Ag)FE in the concentration range from 0.05 to 5.00 $\mu $M. Precision, repeatability, and accuracy of …