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Validation Of Hplc Method For The Determination Of Chemical And Radiochemical Purity Of A 68ga-Labelled Euk-Sub-Kf-(3-Iodo-Y-) Dotaga, Ayşe Uğur, Şükrü Gökhan Elçi̇, Doğangün Yüksel
Validation Of Hplc Method For The Determination Of Chemical And Radiochemical Purity Of A 68ga-Labelled Euk-Sub-Kf-(3-Iodo-Y-) Dotaga, Ayşe Uğur, Şükrü Gökhan Elçi̇, Doğangün Yüksel
Turkish Journal of Chemistry
The prostate-specific membrane antigen (PSMA) represents an ideal biomarker for molecular imaging. Various PSMA-targeted radioligands are available for prostate cancer imaging. In this study, labeling of PSMA I&T with 68Ga, as well as validation of the radiochemical purity of the synthesis product by reverse phase radio high-performance liquid chromatography (HPLC) method are intended. Since the standard procedure for the quality control (QC) was not available, definition of chemical and radiochemical purity of 68Ga-PSMA I&T was carried out according to the Q2 (R1) ICH guideline. The standard QC tests were analyzed with Scintomics 8100 radio-HPLC system equipped with a radioactivity detector. …
Fabrication And Characterization Of Amidoxime-Functionalized Silica Decorated Withcopper: A Catalytic Assembly For Rapid Reduction Of Dyes, Ijaz Ahmed Khan, Muhammad Inaam-Ul-Hassan, Hazrat Hussain, Syed Mujtaba Shah, Tariq Yasin
Fabrication And Characterization Of Amidoxime-Functionalized Silica Decorated Withcopper: A Catalytic Assembly For Rapid Reduction Of Dyes, Ijaz Ahmed Khan, Muhammad Inaam-Ul-Hassan, Hazrat Hussain, Syed Mujtaba Shah, Tariq Yasin
Turkish Journal of Chemistry
In this study, amidoxime-functionalized silica decorated with copper (AFS-Cu) was fabricated and tested for its catalytic application. Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction were employed to characterize its structure and morphology. The application of AFS-Cu as a catalyst for the catalytic reduction of methylene blue (MB) in aqueous media using NaBH$_{4}$ as reductant was evaluated. The ability to reuse as well as the effect of catalyst dose and pH of solution on the catalytic activity was investigated. The reduction of MB followed pseudo-first-order kinetics and the rate constant (k) was 0.6224 min$^{-1}$. …
The Protective Role Of Melatonin Under Heavy Metal-Induced Stress In Melissa Officinalis L., Elvisa Hodzic, Semira Galijasevic, Milica Balaban, Sebila Rekanovic, Halid Makic, Biljana Kukavica, Dijana Mihajlovic
The Protective Role Of Melatonin Under Heavy Metal-Induced Stress In Melissa Officinalis L., Elvisa Hodzic, Semira Galijasevic, Milica Balaban, Sebila Rekanovic, Halid Makic, Biljana Kukavica, Dijana Mihajlovic
Turkish Journal of Chemistry
Heavy metals, due to their inability to degrade, pose a serious environmental and nutritional problem. The accumulation of essential and non-essential heavy metals in living organisms reduces normal growth and development, resulting in acute poisoning, disease and even death of organisms. Melatonin is a very important multifunctional molecule in protecting plants from oxidative stress due to its ability to directly neutralize reactive oxygen species (ROS). Also, melatonin has a chelating property, which may contribute in reducing metal-induced toxicity. In this paper, the protective role of melatonin in counteracting metal-induced free radical generation was highlighted. Using the HPLC-FLD technique melatonin was …
Evaluation Of The Surface Properties Of 4-(Decyloxy) Benzoic Acid Liquid Crystal And Its Use In Structural Isomer Separation, Bi̇rol Işik, Fati̇h Çakar, Hüsnü Cankurtaran, Özlem Cankurtaran
Evaluation Of The Surface Properties Of 4-(Decyloxy) Benzoic Acid Liquid Crystal And Its Use In Structural Isomer Separation, Bi̇rol Işik, Fati̇h Çakar, Hüsnü Cankurtaran, Özlem Cankurtaran
Turkish Journal of Chemistry
The selectivity of 4-(Decyloxy) benzoic acid (DBA) liquid crystal in surface adsorption region (303.2-328.2 K) and thermodynamic region (423.2 - 433.2 K) was investigated by inverse gas chromatography at infinite dilution (IGC-ID). The selectivity parameters of the structural isomer series named butyl acetate, butyl alcohol, and amyl alcohol series were calculated for the DBA using IGC-ID technique. Additionally, the surface properties including dispersive surface energy (γ SD), free energy (ΔGAS), enthalpy (ΔHAS), and acidity-basicity constants were calculated with net retention volumes obtained from IGC-ID experiment results. When the ΔHAS and ΔGAS are constants, DBA surface was found to be an …
One-Pot Green Synthesized Protein-Based Silver Nanocluster As Prooxidant Biosensor, Esi̇n Akyüz
One-Pot Green Synthesized Protein-Based Silver Nanocluster As Prooxidant Biosensor, Esi̇n Akyüz
Turkish Journal of Chemistry
In this study, silver nanoclusters as prooxidant biosensor were eco-friendly synthesized using chicken egg white protein without any chemical reducing agents for measuring copper(II)-induced prooxidant activities of catechin, epicatechin, epigallocatechin gallate, resveratrol, gallic acid, chlorogenic acid, and rutin. The prooxidant activities were evaluated via measuring the absorption at 450 nm wavelength of the Cu(I)-neocuproine chelate formed by extraction of protein-bound Cu(I) with neocuproine reagent. Accuracy was determined by evaluating recovery values of wine, grape and apple samples and the obtained values were between 97.2%-98.9%. Intraday precision and inter-day reproducibility experiments were studied with three different experiments in a day and …
Synthesis Of Imidazo-1,4-Oxazinone Derivatives And Investigation Of Reaction Mechanism, Volkan Taşdemi̇r
Synthesis Of Imidazo-1,4-Oxazinone Derivatives And Investigation Of Reaction Mechanism, Volkan Taşdemi̇r
Turkish Journal of Chemistry
In this study, nine different C-2 aroyl imidazole derivatives were synthesized in a one pot reaction with two steps, and the reduction reactions of these derivatives with NaBH4 were carried out under mild conditions. Substitution reaction of obtained imidazo methanol derivatives with chloroacetylchloride reagent and ring reaction of substitution products were investigated. It was determined that 1,4-imidazoxazinone derivative was obtained as a result of the cyclization reaction. The intermediate products obtained during the cyclization reaction were isolated, and the path of the reaction under different conditions was discussed.
The Involvement Of The Induction Of Anthocyanin Biosynthesis And Transport In Toxic Boron Responsive Regulation In Arabidopsis Thaliana, Ceyhun Kayihan
The Involvement Of The Induction Of Anthocyanin Biosynthesis And Transport In Toxic Boron Responsive Regulation In Arabidopsis Thaliana, Ceyhun Kayihan
Turkish Journal of Botany
Recently, boron (B) has been found to form a complex with anthocyanin, which could be evidence for the B tolerance mechanisms that reduce free B in the leaf tissues of plants. However, the molecular mechanism of anthocyanin biosynthesis and transport has not been satisfactorily elucidated in plants exposed to toxic B. Therefore, the changes in expression levels of some of the phenylpropanoid pathway genes, early and late flavonoid biosynthetic genes, and transcription factors related to anthocyanin biosynthesis and transport were determined in Arabidopsis thaliana under B toxicity. Accordingly, 1 mM boric acid treatment did not cause any significant change in …
Genetic Structure Of Zannichellia L. Populations According To Mountain Barriers In Iranas Revealed By Issr And Srap Markers, Shabnam Abbasi, Saeed Afsharzadeh, Mehri Dinarvand
Genetic Structure Of Zannichellia L. Populations According To Mountain Barriers In Iranas Revealed By Issr And Srap Markers, Shabnam Abbasi, Saeed Afsharzadeh, Mehri Dinarvand
Turkish Journal of Botany
Zannichellia L. (Potamogetonaceae) is a widespread aquatic genus that is distributed in temperate and subtropical aquatic ecosystems worldwide. Because the biogeographical location of Iran includes mountain ranges and deserts that may cause different habitat types, it was assumed that these Zannichellia populations would be stronger differentiated than observed in Zannichellia from temperate regions. The present study aimed to test whether Z. palustris would demonstrate high levels of genetic differentiation over long distances and across mountain ranges. The genetic structure of the populations of Zannichellia was investigated using inter simple sequence repeats (ISSR) and sequence-related amplified polymorphism (SRAP) as molecular markers. …
Chaetocin Enhances Callus Induction By Decreasing The Expression Of Major Leaf Polarity Genes In Nicotiana Tabacum, Ayyub Ebrahimi, Nagi̇han Özsoy, Saadet Deni̇z Gürle, Baki̇ Yaman, Şule Ari
Chaetocin Enhances Callus Induction By Decreasing The Expression Of Major Leaf Polarity Genes In Nicotiana Tabacum, Ayyub Ebrahimi, Nagi̇han Özsoy, Saadet Deni̇z Gürle, Baki̇ Yaman, Şule Ari
Turkish Journal of Botany
Using chemicals known as inhibitors of chromatin remodeling enzymes is a useful approach in understanding the regulation machinery that contributes to the maintenance of or conversion to a pluri- and totipotent states of the cells in multicellular organisms. They can serve as tools to reveal the molecular and cellular mechanisms required for reprogramming. A deeper understanding of reprogramming pathways that drive the cell into a dedifferentiated state might help us enhance plant micropropagation and green biotechnology applications. Chaetocin is one of the histone methyltransferase inhibitors known as key drivers in several cellular processes. It was shown that chaetocin enhanced the …
Puerarin-Coated Gold Nanoparticles (Pue-Aunps) Synthesized Via Green Synthetic Route: A New Colorimetric Probe For The Detection Of Ciprofloxacin, Tasneem Zehra, Faiqa Ahsan, Muhammad Ali Versiani, Sana Wahid, Sajid Jahangir, Muhammad Raza Shah
Puerarin-Coated Gold Nanoparticles (Pue-Aunps) Synthesized Via Green Synthetic Route: A New Colorimetric Probe For The Detection Of Ciprofloxacin, Tasneem Zehra, Faiqa Ahsan, Muhammad Ali Versiani, Sana Wahid, Sajid Jahangir, Muhammad Raza Shah
Turkish Journal of Chemistry
Puerarin-coated gold nanoparticles (PUE-AuNPs) synthesized via green synthetic route, a new colorimetric sensor, efficiently detected the ciprofloxacin (CP) in tap water and cow milk samples. The PUE-AuNPs were characterized by UV-visible, FTIR, AFM, and DLS techniques and were found to be spherical with an average size of approximately 19-20 nm. FTIR spectrum confirms that functional groups such as -OH, -C=O, -CO and -C=C were responsible for the reduction of gold (III) chloride trihydrate. These functional groups acted as capping agents to form AuNPs. The PUE-AuNPs sensor was proved to be selective and sensitive for the detection of CP through colorimetric …
A Fluorescent Sensor-Based Tripodal-Bodipy For Cu (Ii) Ions: Bio-Imaging On Cells, Ahmed Nuri̇ Kurşunlu, Mustafa Özmen, Ersi̇n Güler
A Fluorescent Sensor-Based Tripodal-Bodipy For Cu (Ii) Ions: Bio-Imaging On Cells, Ahmed Nuri̇ Kurşunlu, Mustafa Özmen, Ersi̇n Güler
Turkish Journal of Chemistry
A general synthetic method was improved to synthesize a chemosensor based on a tripodal Bodipy (t-BODIPY) structure. The product and its intermediate products were successfully prepared and fully characterized. The metal ion sensing performance of the tripodal compound was evaluated by UV/Vis and fluorescence spectroscopies. According to the obtained data, t-BODIPY is a selective and sensitive fluorescence probe for detection of Cu2+ ions in the presence and in the absence of competing ions. This chemosensor presents relatively a low detection limit of 5.4 x10-7 M for Cu2+. Bio-imaging studies on living yeast cells suggest that t-BODIPY has some advantages over …
Detailed Evaluation Of Cancer Sequencing Pipelines In Different Microenvironments And Heterogeneity Levels, Batuhan Kisakol, Şahi̇n Sarihan, Mehmet Ari̇f Ergün, Mehmet Baysan
Detailed Evaluation Of Cancer Sequencing Pipelines In Different Microenvironments And Heterogeneity Levels, Batuhan Kisakol, Şahi̇n Sarihan, Mehmet Ari̇f Ergün, Mehmet Baysan
Turkish Journal of Biology
The importance of next generation sequencing (NGS) rises in cancer research as accessing this key technology becomes easier for researchers. The sequence data created by NGS technologies must be processed by various bioinformatics algorithms within a pipeline in order to convert raw data to meaningful information. Mapping and variant calling are the two main steps of these analysis pipelines, and many algorithms are available for these steps. Therefore, detailed benchmarking of these algorithms in different scenarios is crucial for the efficient utilization of sequencing technologies. In this study, we compared the performance of twelve pipelines (three mapping and four variant …
Targeting The Tumor Stroma: Integrative Analysis Reveal Gata2 And Toryaip1 As Novel Prognostic Targets In Breast And Ovarian Cancer, Ömer Faruk Erceylan, Ayşe Savaş, Esra Göv
Targeting The Tumor Stroma: Integrative Analysis Reveal Gata2 And Toryaip1 As Novel Prognostic Targets In Breast And Ovarian Cancer, Ömer Faruk Erceylan, Ayşe Savaş, Esra Göv
Turkish Journal of Biology
Tumor stroma interaction is known to take a crucial role in cancer growth and progression. In the present study, it was performed gene expression analysis of stroma samples with ovarian and breast cancer through an integrative analysis framework to identify common critical biomolecules at multiomics levels. Gene expression datasets were statistically analyzed to identify common differentially expressed genes (DEGs) by comparing tumor stroma and normal stroma samples. The integrative analyses of DEGs indicated that there were 59 common core genes, which might be feasible to be potential marks for cancer stroma targeted strategies. Reporter molecules (i.e. receptor, transcription factors and …
Positive Regulation Of Tnfr1 Signaling Via Sh3 Recognition Motif, Fatma Ece Çopuroğlu, Fatma Zehra Hapi̇l Zevkli̇ler, Şükran Burçak Yoldaş, Osman Ni̇dai̇ Özeş
Positive Regulation Of Tnfr1 Signaling Via Sh3 Recognition Motif, Fatma Ece Çopuroğlu, Fatma Zehra Hapi̇l Zevkli̇ler, Şükran Burçak Yoldaş, Osman Ni̇dai̇ Özeş
Turkish Journal of Biology
TNF is a pleiotropic cytokine and shows its biological function by binding to its receptors called TNFR1 and TNFR2. While TNFR1 induces apoptosis by activation of caspase-8 via the "death domain", it also activates IKKα/β, MKK3/6, MKK4/7 by activation of TAK1. Although the TNFR1 signaling pathway is known by in large, it is not known how AKT and MAPKs p38, ERK1/2, and JNK1/2 are activated. The presence of a proline-rich PPAP region, (P448PAP451, a binding site for the SH3 domain-containing proteins) very close to the C-terminus promoted us to determine whether this region has any role in the TNFR1 signal …
Negative Regulation Of Diminutive Cancer Regulator Through Differentiation And Microrna Pathway Components In Drosophila Cells, Sumira Malik
Negative Regulation Of Diminutive Cancer Regulator Through Differentiation And Microrna Pathway Components In Drosophila Cells, Sumira Malik
Turkish Journal of Biology
Drosophila model is intensively studied for the development of cancer. The diminutive (dMyc), a homolog of the human MYC gene, is responsible for cell- apoptosis and its upregulation is responsible for determining the fate of cancerous growth in humans and Drosophila model. This work implores the requirement of dMyc and its expression as one of the major regulator of cancer with other proteins and repression of dMyc mRNA in Drosophila S2 cells. Here we report protein complex of Argonaute 1 (AGO1), Bag of marbles (Bam), and Brain tumor (Brat) proteins and not the individual factor of this complex repression of …
Compensatory Expression Regulation Of Highly Homologous Proteins Hnrnpa1 Andhnrnpa2, Yan Chang, Xiao Feng Lu, Jia Ying Qiu
Compensatory Expression Regulation Of Highly Homologous Proteins Hnrnpa1 Andhnrnpa2, Yan Chang, Xiao Feng Lu, Jia Ying Qiu
Turkish Journal of Biology
Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains. Transfection of HEK293 cells with small interfering RNA and overexpression vectors of HNRNPA1 and HNRNPA2 demonstrated that HNRNPA1 and HNRNPA2 paralogs regulate each other?s expression in a compensatory manner at …
Synthesis Of Intracellular And Extracellular Gold Nanoparticles With A Green Machine And Its Antifungal Activity, Nurbanu Gürsoy, Betül Yilmaz Öztürk, İlknur Dağ
Synthesis Of Intracellular And Extracellular Gold Nanoparticles With A Green Machine And Its Antifungal Activity, Nurbanu Gürsoy, Betül Yilmaz Öztürk, İlknur Dağ
Turkish Journal of Biology
Green synthesis method is being increasingly used in the development of safe, stable, and eco-friendly nanostructures with biological resources. In this study, extracellular and intracellular synthesis of gold nanoparticles (AuNPs) was carried out using green algae Chlorella sorokiniana Shihira & R.W. Fresh algae were isolated and identified from Musaözü Pond located in the province of Eskişehir and then extraction process were performed. Optimization studies were studied using pH value, metal salt concentration, and time parameters for extracellular synthesis and using only time parameter for intrasellular synthesis. Since more controlled and optimum conditions can be achieved in the production of AuNPs …
Il-1r1 Deficiency Impairs Liver Regeneration After 2/3 Partial Hepatectomy In Aged Mice, Deming Li, Ze Wang, Chunyan Zhang, Cunshuan Xu
Il-1r1 Deficiency Impairs Liver Regeneration After 2/3 Partial Hepatectomy In Aged Mice, Deming Li, Ze Wang, Chunyan Zhang, Cunshuan Xu
Turkish Journal of Biology
Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the IL-1R1 knockout (KO, n = 36) 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative realtime polymerase chain reaction (qRT-PCR) and Western blotting methods, respectively. Compared with WT mice, liver coefficient …
Reconstitution Of Pol Ii (G) Responsive Form Of The Human Mediator Complex, Murat Alper Cevher
Reconstitution Of Pol Ii (G) Responsive Form Of The Human Mediator Complex, Murat Alper Cevher
Turkish Journal of Biology
RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription …
Polyhydroxyalkanoate Accumulation In Streptomyces Coelicolor Affected By Sco7613 Gene Region, Zeynep Demi̇r Öksüz, Tuğrul Doruk, Nevi̇n Yağci, Sedef Tunca Gedi̇k
Polyhydroxyalkanoate Accumulation In Streptomyces Coelicolor Affected By Sco7613 Gene Region, Zeynep Demi̇r Öksüz, Tuğrul Doruk, Nevi̇n Yağci, Sedef Tunca Gedi̇k
Turkish Journal of Biology
Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome. BLAST search results of the GenBank database argued that SCO7613 could specify a putative polyhydroxyalkanoate synthase (PhaC) responsible for PHA biosynthesis. Deduced amino acid sequence of SCO7613 showed the presence of conserved lipase box like sequence, …
Lipase From New Isolate Bacillus Cereus Ata179: Optimization Of Production Conditions, Partial Purification, Characterization And Its Potential In The Detergent Industry, Eli̇f Demi̇rkan, Aynur Aybey Çeti̇nkaya, Maoulida Abdou
Lipase From New Isolate Bacillus Cereus Ata179: Optimization Of Production Conditions, Partial Purification, Characterization And Its Potential In The Detergent Industry, Eli̇f Demi̇rkan, Aynur Aybey Çeti̇nkaya, Maoulida Abdou
Turkish Journal of Biology
In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NH4)2HPO4 as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45°C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h. ATA179 strain showed a 51% increase in enzyme production in the modified medium created by optimizing nutritional …
Transcriptome Profiling Of Stevia Rebaudiana Ms007 Revealed Genes Involved In Flower Development, Nurul Hidayah Samsulrizal, Khairul Shahyidi Khadzran, Tamil Sundram Meenakshi Sundram, Zarina Zainuddin, Siti Hajar Nor Shaarani, Nur Sabrina Ahmad Azmi, Sarahani Harun
Transcriptome Profiling Of Stevia Rebaudiana Ms007 Revealed Genes Involved In Flower Development, Nurul Hidayah Samsulrizal, Khairul Shahyidi Khadzran, Tamil Sundram Meenakshi Sundram, Zarina Zainuddin, Siti Hajar Nor Shaarani, Nur Sabrina Ahmad Azmi, Sarahani Harun
Turkish Journal of Biology
Stevia rebaudiana is a medicinal plant recommended to diabetic or obese patients as an alternative sweetener owing to its low-calorie property. Previous studies have found that the stevioside level is highest at the time of flower bud formation and lowest at the time of preceding and following flower bud formation. Hence, this study aims to identify the genes involved in the flowering of local S. rebaudiana accession MS007 by investigating the transcriptomic data of two stages of growth, before flowering (BF) and after flowering (AF) that were deposited under accession number SRX6362785 and SRX6362784 at the NCBI SRA database. The …
Linkage Of Nanosecond Protein Motion With Enzymatic Methyl Transfer By Nicotinamide N-Methyltransferase, Yahui Jing, Yiting Cheng, Fangya Li, Yuping Li, Fan Liu, Jianyu Zhang
Linkage Of Nanosecond Protein Motion With Enzymatic Methyl Transfer By Nicotinamide N-Methyltransferase, Yahui Jing, Yiting Cheng, Fangya Li, Yuping Li, Fan Liu, Jianyu Zhang
Turkish Journal of Biology
Nicotinamide N-methyltransferase (NNMT), a key cytoplasmic protein in the human body, is accountable to catalyze the nicotinamide (NCA) N1-methylation through S-adenosyl-L-methionine (SAM) as a methyl donor, which has been linked to many diseases. Although extensive studies have concerned about the biological aspect, the detailed mechanism study of the enzyme function, especially in the part of protein dynamics is lacking. Here, wild-type nicotinamide N-methyltransferase together with the mutation at position 20 with Y20F, Y20G, and free tryptophan were carried out to explore the connection between protein dynamics and catalysis using time-resolved fluorescence lifetimes. The results show that wild-type nicotinamide N-methyltransferase prefers …
Characteristics And Clinical Significance Of Cd163+/Cd206+M2 Mono-Macrophage In The Bladder Cancer Microenvironment, Xiangjie Kong, Ming Zhu, Zhirong Wang, Zhuoqun Xu, Jianfeng Shao
Characteristics And Clinical Significance Of Cd163+/Cd206+M2 Mono-Macrophage In The Bladder Cancer Microenvironment, Xiangjie Kong, Ming Zhu, Zhirong Wang, Zhuoqun Xu, Jianfeng Shao
Turkish Journal of Biology
The tumor microenvironment may recruit monocytes, with a protumoral macrophage phenotype (M2) that plays an important role in solid tumor progression and metastasis. Therefore, it is necessary to understand the characteristics of these cells for cancer prevention and treatment. Bladder cancer tissue samples and paracarcinoma tissues samples were collected, and the expression of CD163+ cells in tumor tissues was observed. Then, we observed the expression of infiltrating CD45+CD14+CD163+ cell subset and analyzed the molecular expressions related to immunity and angiogenesis. C57/BL6 mice were inoculated subcutaneously, and dynamic changes of CD11b+F4/80+CD206+ mononuclear macrophages expression for tumor-bearing mice were detected. The results …
Screening Of The Small Molecule Library Of Meinox Enables The Identification Of Anticancer Compounds In Pathologically Distinct Cancers, Aynura Mammadova, Ari̇f Mermer, Fati̇h Kocabaş
Screening Of The Small Molecule Library Of Meinox Enables The Identification Of Anticancer Compounds In Pathologically Distinct Cancers, Aynura Mammadova, Ari̇f Mermer, Fati̇h Kocabaş
Turkish Journal of Biology
Small molecules are widely used for the modulation of the molecular basis of diseases. This makes them the perfect tool for discovering and developing new therapeutics. In this work, we have established a library of small molecules in house and characterized its molecular and druglike properties. We have shown that most small molecules have molecular weights less than 450. They have pharmaceutically relevant cLogP, cLogS, and druglikeness value distributions. In addition, Meinox's small molecule library contained small molecules with polar surface areas that are less than 60 square angstroms, suggesting their potent ability to cross the blood-brain barrier. Meinox's small …
Investigation Of Neuro-Inflammatory Parameters In A Cuprizone Induced Mouse Model Of Multiple Sclerosis, Ti̇muçi̇n Avşar, Gökçe Erdem, Gökhan Terzi̇oğlu, Eda Tahi̇r Turanli
Investigation Of Neuro-Inflammatory Parameters In A Cuprizone Induced Mouse Model Of Multiple Sclerosis, Ti̇muçi̇n Avşar, Gökçe Erdem, Gökhan Terzi̇oğlu, Eda Tahi̇r Turanli
Turkish Journal of Biology
Cuprizone, copper chelator, treatment of mouse is a toxic model of multiple sclerosis (MS) in which oligodendrocyte death, demyelination and remyelination can be observed. Understanding T and B cell subset as well as their cytokines involved in MS pathogenesis still requires further scrutiny to better understand immune component of MS. The study presented here, aimed to evaluate relevant cytokines, lymphocytes, and gene expressions profiles during demyelination and remyelination in the cuprizone mouse model of MS. Eighty male C57BL/6J mice fed with 0.2% cuprizone for eight weeks. Cuprizone has been removed from the diet in the following eight weeks. Cuprizone treated …