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Influence Of Different Proteinaceous Diets On Consumption, Brood Rearing,And Honey Bee Quality Parameters Under Isolation Conditions, Abdulraouf Amro, Mohamed Omar, Ahmed Al-Ghamdi
Influence Of Different Proteinaceous Diets On Consumption, Brood Rearing,And Honey Bee Quality Parameters Under Isolation Conditions, Abdulraouf Amro, Mohamed Omar, Ahmed Al-Ghamdi
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to evaluate the efficacy of 5 proteinaceous diets on honey bees under isolation conditions. The diets were soybean meal, mesquite pod powder, date paste, Feedbee®, and corn gluten, along with control colonies (bees fed naturally on pollens). These 5 diets were supplied to honey bee colonies under isolation conditions, except for the control group. Food consumption amount, brood rearing activity, and some physiological parameters of the honey bees were measured. The best consumption amount was for date paste, followed by Feedbee, mesquite, and corn gluten, respectively. Concerning brood rearing activity, the highest mean of sealed …
Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam
Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the effects of diet and exogenous enzymes on growth responses and micronutrient digestibility of broilers. Day-old broilers (n = 256) were distributed into a 2 x 2 factorial design and were fed on two basal diets: soybean (T1) or canola (T2) meals as such, or supplemented with enzymes up to 21 days. Feed intake (FI) and live weight (LW) to 21 days on the T2 diet was higher (P < 0.001) than that on T1. Feed conversion ratio (FCR) was better (P < 0.01) on T1 than on T2 at 21 days. Enzymes generally improved (P < 0.001) FI, FCR, and LW. The digestibility of histidine was higher (P < 0.05) on T2, but lysine digestibility was higher (P < 0.01) on T1 at 21 days. Histidine, threonine, lysine, valine, isoleucine, and phenylalanine digestibility was improved (P < 0.05) in the supplemented diets. Mineral digestibility was unaffected (P > 0.05) by diet, but enzymes increased (P < 0.05) digestibility of P, K, Mn, and Cu at 21 days. The digestibility of Cu, Zn, and Mg was higher (P < 0.05) on T2, whereas Ca digestibility was greater (P < 0.05) on the T1 diet. Broilers' growth responded positively to enzyme diets, probably due to improvement in nutrient digestibility.
Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu
Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu
Turkish Journal of Veterinary & Animal Sciences
Metabolic diseases such as enzootic ataxia, white muscle disease, and white liver disease are among the most important problems of livestock for sheep. The aim of this study was to analyze whether tears can be used as an alternative diagnostic method in determination of diseases that result from lack or excess of copper, selenium, and cobalt. Samples of serum and tears taken from 45 healthy sheep of the Kivircik breed aged between 2 and 4 years were analyzed. The concentrations of copper, selenium, and cobalt were measured in serum and tears as 118.0 ± 36.0, 58.0 ± 15.0, and 0.5 …
Investigation Of Serum Testosterone Level, Scrotal Circumference, Body Mass,Semen Characteristics, And Their Correlations In Developing Mis Lambs, Nevena Maksimovic, Slavca Hristov, Branislav Stankovic, Milan P. Petrovic, Cvijan Mekic, Dragana Ruzic-Muslic, Violeta Caro-Petrovic
Investigation Of Serum Testosterone Level, Scrotal Circumference, Body Mass,Semen Characteristics, And Their Correlations In Developing Mis Lambs, Nevena Maksimovic, Slavca Hristov, Branislav Stankovic, Milan P. Petrovic, Cvijan Mekic, Dragana Ruzic-Muslic, Violeta Caro-Petrovic
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate changes of serum testosterone, scrotal circumference, body weight, and semen characteristics in a Meat Institute Sheep (MIS) population during their developing period (between 3 and 17 months). Correlative relationships among parameters were also tested. Body weight and scrotal circumference were measured monthly, blood samples for testosterone levels were taken bimonthly (3-17 months), and semen samples were collected by an electroejaculator between the ages of 9 and 17 months. The analysis showed that the average serum testosterone levels ranged from 1.83 to 13.28 ng/mL and significantly depended on the age of the lambs …
Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala
Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala
Turkish Journal of Veterinary & Animal Sciences
In vitro fertilization is a common method of fertilization in the case of both humans and animals. Although this method has developed very rapidly, there are still many unanswered questions about the optimal characteristics that oocytes should have for performing this procedure. The object of this study was to describe the correlation between the diameter and size of swine oocytes in relation to pubescence. The research was conducted on ovaries of piglets (n = 65) and adult swine (n = 69). Each oocyte was examined with and without the zona pellucida. The analysis showed that the average size of the …
Chemical And Tissue Composition Of Meat From Carcass Cuts Of Localgoats Affected By Different Feeding In Tunisian Arid Lands, Naziha Ayeb, Asma Ghrab, Ahmed Barmat, Touhami Khorchani
Chemical And Tissue Composition Of Meat From Carcass Cuts Of Localgoats Affected By Different Feeding In Tunisian Arid Lands, Naziha Ayeb, Asma Ghrab, Ahmed Barmat, Touhami Khorchani
Turkish Journal of Veterinary & Animal Sciences
An experiment was conducted to find out the nutritive value of different cuts of goat carcass. Meat samples were obtained from goat kids in Tunisia. Twenty-six indigenous kids were divided into three groups: oat hay (control), dried olive leaves + dried Stipa tenacissima, and grass hay. The cuts were the shoulder, chest, and leg of each goat carcass. Tissue compositions of different cuts and meat physicochemical parameters were determined. Percentage of muscle was higher in shoulders (61.57%) and legs (58.89%). The percentage of fat was higher in the chest (24.74%). Percentage of bone was higher in legs and shoulders. The …
Effect Of Oxytocin On Milk Proteins And Fatty Acid Profile Insahiwal Cows During Lactation Periods, Aneela Hameed, Faqir Muhammad Anjum, Tahir Zahoor, Zia Ur-Rahman, Saeed Akhtar, Majid Hussain
Effect Of Oxytocin On Milk Proteins And Fatty Acid Profile Insahiwal Cows During Lactation Periods, Aneela Hameed, Faqir Muhammad Anjum, Tahir Zahoor, Zia Ur-Rahman, Saeed Akhtar, Majid Hussain
Turkish Journal of Veterinary & Animal Sciences
Bovine milk, a rich source of proteins and fatty acids, has a beneficial impact on physicochemical and organoleptic properties. The current study was planned to identify oxytocin's effects on protein and fatty acid profiles of cow milk (the Sahiwal breed) at various lactation stages (early, middle, and late) under a controlled atmosphere and feeding inputs. Examination of milk protein and fatty acid profiles by urea polyacrylamide gel electrophoresis and gas chromatography-mass spectrometry respectively revealed the significant effect (P < 0.01) of oxytocin during the cow's lactation stages. In electrophoretic patterns, casein (αs1-CN, αs2-CN, β-CN, and k-CN) and whey protein fractions (Ig, BSA, β-Lg, and α-La) appeared as low-intensity bands in oxytocin-injected milk when compared to the control. Reductions in concentrations of fatty acids C16:0 (25.00%) and C18:1 (21.60%) were observed in oxytocin-treated milk as compared to the control group (29.30% and 29.10%, respectively) at late lactation.
The Treatment Of Distal Metacarpus Fracture With Locking Compression Plate In Calves*, Ali̇ Belge, İbrahi̇m Akin, Ali̇ Gülaydin, Muhammed Fati̇h Yazici
The Treatment Of Distal Metacarpus Fracture With Locking Compression Plate In Calves*, Ali̇ Belge, İbrahi̇m Akin, Ali̇ Gülaydin, Muhammed Fati̇h Yazici
Turkish Journal of Veterinary & Animal Sciences
In this study, we aimed to evaluate the effect of locking compression plates for the treatment of distal metacarpal fractures in calves. The metacarpus was stabilized using a stainless steel locking compression plate with fully threaded locking head screws. The calves started weight-bearing partially after the first postoperative day and resumed complete weight-bearing after day 10. Callus formation was obtained at the end of the 3rd week and completed at the end of the 8th week, and complete healing was seen at the 12th week in radiographs. The limbs were in good alignment, the calves were fully weight-bearing, and client …
Cryopreservation Of Seabream (Sparus Aurata) Semen And Fertility, Mehmet Borga Tirpan, Erkut Dervi̇ş Özgöray, Havva Alemdar, Ergün Akçay
Cryopreservation Of Seabream (Sparus Aurata) Semen And Fertility, Mehmet Borga Tirpan, Erkut Dervi̇ş Özgöray, Havva Alemdar, Ergün Akçay
Turkish Journal of Veterinary & Animal Sciences
In this study, the aim was to reveal spermatological parameters of seabream (Sparus aurata), freezing ability of semen in different extenders, and fertilization capacity of frozen seabream semen. The study was conducted with 10 male and 6 female brooder seabreams. Female fish were synchronized by photoperiod and water temperature in the breeding season. Four different extenders [Mounib, Salty Mounib, Ringer, and sea water (1% NaCl)] including 10% dimethyl sulfoxide were used to assess the freezing ability of seabream semen. After postthaw spermatological examinations, three successful extenders (Mounib, Ringer, 1% NaCl) were chosen for the fertilization stage and 2 control and …
Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial
Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial
Turkish Journal of Veterinary & Animal Sciences
The activity of enzymes that digest carbohydrates at various points in the gastrointestinal tract of sheep was examined. In addition, weight and contents of digestive tract segments were examined. The experiment was performed on six adult ewes fed an 85% hay diet. The morphometric measurements revealed that the alimentary tract constituted 32% of the sheep's body mass and wet rumen contents constituted 70% of wet mass of the total digestive tract content. The protozoal population in the rumen varied from 37.2 × 104 to 54.0 × 104/mL rumen fluid. In the examined samples, ciliates were identified as belonging to the …
Chromium Redox Speciation In Food Samples, Krystyna Pyrzynska
Chromium Redox Speciation In Food Samples, Krystyna Pyrzynska
Turkish Journal of Chemistry
Chromium is an element with important biological characteristics, depending on its different species. Cr(III) is considered as essential; however, the Cr(VI) form is classified as carcinogenic.~For this reason, speciation analysis in food samples is a very important question. The proposed data procedures for chromium redox speciation in the literature can be divided into those with the use of online hyphenated techniques and those where appropriate sample pretreatment is necessary. The strategies for nonchromatographic speciation are mainly based on selective liquid--liquid extraction procedures, coprecipitation, selective separation of chromium species using a solid phase extraction column, or complexation reactions. In this paper, …
Comparative Density Functional Study Of Antioxidative Activity Of The Hydroxybenzoic Acids And Their Anions, Zoran Markovic, Jelena Djorovic, Jasmina M. Dimitric Markovic, Radomir Biocanin, Dragan Amic
Comparative Density Functional Study Of Antioxidative Activity Of The Hydroxybenzoic Acids And Their Anions, Zoran Markovic, Jelena Djorovic, Jasmina M. Dimitric Markovic, Radomir Biocanin, Dragan Amic
Turkish Journal of Chemistry
Hydroxybenzoic acids (HBAs) and their anions play an important role in the food and pharmaceutical industries because of their antioxidant activity. In this study, we examined the mechanisms of the free radical scavenging action of HBAs and their anions using density functional theory (DFT) methods. Reaction enthalpies related to the mechanisms of free radical scavenging by the investigated species were calculated by DFT methods in water, DMSO, pentylethanoate, and benzene. Hydrogen atom transfer (HAT) is a preferred reaction pathway in benzene, while sequential proton loss electron transfer (SPLET) is a predominant reaction pathway in polar solvents, water, and DMSO for …
Speciation Of Inorganic Selenium By Solid Phase Extraction Using Nanozirconium Oxide/Boron Oxide Composite Material, Hakan Erdoğan, Özcan Yalçinkaya, Ali̇ Rehber Türker
Speciation Of Inorganic Selenium By Solid Phase Extraction Using Nanozirconium Oxide/Boron Oxide Composite Material, Hakan Erdoğan, Özcan Yalçinkaya, Ali̇ Rehber Türker
Turkish Journal of Chemistry
The speciation and preconcentration of selenium were carried out by solid phase extraction using nanozirconium oxide/boron oxide (ZrO$_{2}$/B$_{2}$O$_{3})$. Electrothermal atomic absorption spectrometry (ETAAS) was used for detection. This preconcentration and/or speciation method depends on the retention of Se(IV) ions selectively as its ammonium pyrrolidine dithiocarbamate (APDC) complex on the sorbent. The effects of pH, flow rate, and volume of sample solution and eluent type were investigated for determining the optimum preconcentration and speciation conditions. The effects of other ions that may interfere with the determination and speciation of selenium were also investigated. Moreover, the analytical performance criteria of the method …
Mn-Doped Zns Quantum Dots As A Room-Temperature Phosphorescent Probe For Analysis Of Glutamic Acid In Foodstuffs, Hayri̇ye Eda Şatana Kara, Burak Demi̇rhan, Buket Er Demi̇rhan
Mn-Doped Zns Quantum Dots As A Room-Temperature Phosphorescent Probe For Analysis Of Glutamic Acid In Foodstuffs, Hayri̇ye Eda Şatana Kara, Burak Demi̇rhan, Buket Er Demi̇rhan
Turkish Journal of Chemistry
L-cysteine-capped Mn-doped ZnS quantum dots (QDs) were used for the determination of glutamic acid in foodstuffs. This method is based on measurement of the quenching of the phosphorescence intensity of the QDs after interacting with glutamic acid. A linear response was observed from 50 to 500 ng mL$^{-1}$ glutamic acid with a limit of detection of 6.79 ng mL$^{-1}$. Room temperature phosphorescence (RTP) intensity of the QDs was quenched rapidly upon the addition of the quencher and the reaction reached equilibrium within 2 min. The quenching mechanism of phosphorescence of Mn-doped ZnS QDs by glutamic acid is dynamic and the …
Determination Of Hexavalent Chromium In Aqueous And Alkaline Soil Extracts, Tsvetomil Voyslavov, Stefan Tsakovski, Sonya Ganeva
Determination Of Hexavalent Chromium In Aqueous And Alkaline Soil Extracts, Tsvetomil Voyslavov, Stefan Tsakovski, Sonya Ganeva
Turkish Journal of Chemistry
A fast, simple, and reliable method for determination of hexavalent chromium species in soil aqueous and alkaline extracts has been developed. The procedure is based on the ion-pair extraction of Cr(VI) with methyltrioctylammonium chloride (Aliquat 336) into xylene. The extract is analyzed by flame (FAAS) or electrothermal atomic absorption spectrometry (ETAAS). The method allows the detection of up to 2 mg kg$^{-1}$ Cr(VI) by FAAS and up to 25 $\mu $g kg$^{-1}$ Cr(VI) by ETAAS. The relative standard deviation varied in the range 2%-12% depending on the Cr(VI) levels in the samples. The analysis of certified reference material CRM041-30G Chromium …
Synthetic Protocols On 6$H$-Benzo[$C$]Chromen-6-Ones: A Review, Ofentse Mazimba
Synthetic Protocols On 6$H$-Benzo[$C$]Chromen-6-Ones: A Review, Ofentse Mazimba
Turkish Journal of Chemistry
6$H$-Benzo[$c$]chromen-6-ones serve as core structures of secondary metabolites and are of considerable pharmacological importance. Natural sources produce limited quantities, hence the need for synthetic procedures for 6$H$-benzo[$c$]chromen-6-ones, which are herein reviewed. The literature describes protocols such as the Suzuki coupling reactions for the synthesis of biaryl, which then undergoes lactonization, reactions of 3-formylcoumarin (chromenones) with 1,3-bis(silylenol ethers), radical mediated cyclization of arylbenzoates, metal or base catalyzed cyclization of phenyl-2-halobenzoates and 2-halobenzyloxyphenols, and benzoic acid coupling with benzoquinone using electrophilic metal-based catalyst. The efficient and simple procedures are those involving the reactions of Michael acceptor (chromenones and chalcones) with 1,3- and …
Machilus Thunbergii Extract Inhibits Inflammatory Response Inlipopolysaccharide-Induced Raw264.7 Murine Macrophages Viasuppression Of Nf-$\Kappa$B And P38 Mapk Activation, Shu-Ju Wu, Wen-Bin Len, Chien-Yi Huang, Chian-Jiun Liou, Wen-Chung Huang, Chwan-Fwu Lin
Machilus Thunbergii Extract Inhibits Inflammatory Response Inlipopolysaccharide-Induced Raw264.7 Murine Macrophages Viasuppression Of Nf-$\Kappa$B And P38 Mapk Activation, Shu-Ju Wu, Wen-Bin Len, Chien-Yi Huang, Chian-Jiun Liou, Wen-Chung Huang, Chwan-Fwu Lin
Turkish Journal of Biology
Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells. The cells were pretreated with various doses (12.5 μg/mL-100 μg/mL) of M. thunbergii extract and activated with LPS. We found that 25 μg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor …
Epigallocatechin 3-Gallate Applications On Ht-29 And Mcf-7 Cell Lines Andevaluation Of Tumor Suppressor Gene Methylation, Yunus Kasim Terzi̇, Özge Özer Kaya, Özlem Darcansoy İşeri̇, Zerri̇n Çeli̇k, Feri̇de İffet Şahi̇n
Epigallocatechin 3-Gallate Applications On Ht-29 And Mcf-7 Cell Lines Andevaluation Of Tumor Suppressor Gene Methylation, Yunus Kasim Terzi̇, Özge Özer Kaya, Özlem Darcansoy İşeri̇, Zerri̇n Çeli̇k, Feri̇de İffet Şahi̇n
Turkish Journal of Biology
No abstract provided.
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Turkish Journal of Biology
No abstract provided.
The Effect Of Mart Gene On Biofilm Production Of Salmonella Typhimurium, Burcu Yener İlçe, Nefi̇se Akçeli̇k
The Effect Of Mart Gene On Biofilm Production Of Salmonella Typhimurium, Burcu Yener İlçe, Nefi̇se Akçeli̇k
Turkish Journal of Biology
No abstract provided.
Effects Of A Western-Style Diet High In Cholesterol And Saturated Faton The Rabbit Exocrine Pancreas, María Alba Martínez Burgos, Maria Dolores Yago Torregrosa, Maria Dolores Mesa García, Maria Belen López Millán, Jesús Alcalá Bejarano Carrillo, Ana Rivas Velasco, Ángel Gil Hernández, Mariano Mañas Almendros, Emilio Martínez De Victoria Muñoz
Effects Of A Western-Style Diet High In Cholesterol And Saturated Faton The Rabbit Exocrine Pancreas, María Alba Martínez Burgos, Maria Dolores Yago Torregrosa, Maria Dolores Mesa García, Maria Belen López Millán, Jesús Alcalá Bejarano Carrillo, Ana Rivas Velasco, Ángel Gil Hernández, Mariano Mañas Almendros, Emilio Martínez De Victoria Muñoz
Turkish Journal of Biology
No abstract provided.
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Turkish Journal of Biology
Therapeutic proteins have shown to be effective against a variety of diseases. Pharmaceutical companies are progressively focusing on research using proteins in search of novel effective therapeutics. The significant attention by researchers has resulted in considerable advances in the production and usage of therapeutic proteins in recent years. In the current study we focused on the understanding of therapeutic proteins and their impact on the human health care system. Advancements in the field of biotechnology have increased and facilitated the production of therapeutically significant proteins to combat various fatal diseases. Although protein-based therapeutics have taken center stage in drug discovery …
Aspects Of Mycorrhizal Colonization In Adaptation Of Sweet Marjoram (Origanum Majorana L.) Grown On Industrially Polluted Soil, Marieta Hristozkova, Maria Geneva, Ira Stancheva, Madlen Boychinova, Efrosina Djonova
Aspects Of Mycorrhizal Colonization In Adaptation Of Sweet Marjoram (Origanum Majorana L.) Grown On Industrially Polluted Soil, Marieta Hristozkova, Maria Geneva, Ira Stancheva, Madlen Boychinova, Efrosina Djonova
Turkish Journal of Biology
The current study reveals the effects of mycorrhization on heavy metal uptake and accumulation, antioxidant potential, and essential oil composition of Origanum majorana L., grown on soil polluted with industrial Cd and Pb. Two strains of Claroideoglomus claroideum (EEZ 54 and EEZ 55) were isolated from soil that is naturally rich in metals. EEZ 35 (Funneliformis mosseae) was isolated from a place with industrial contamination. The percentage of mycorrhizal colonization with the EEZ 54 strain was higher than those of the other strains; there was no significant difference between EEZ 55 and EEZ 35. The highest value of the total …
Influence Of Carbon Sources On Growth And Gc-Ms Based Metabolite Profiling Of Arnica Montana L. Hairy Roots, Maria Petrova, Ely Zayova, Ivayla Dincheva, Ilian Badjakov, Mariana Vlahova
Influence Of Carbon Sources On Growth And Gc-Ms Based Metabolite Profiling Of Arnica Montana L. Hairy Roots, Maria Petrova, Ely Zayova, Ivayla Dincheva, Ilian Badjakov, Mariana Vlahova
Turkish Journal of Biology
Arnica montana L. (Asteraceae) is an economically important herb that contains numerous valuable biologically active compounds accumulated in various parts of the plant. The effects of carbon sources (sucrose, maltose, and glucose) at different concentrations (1%, 3%, 5%, 7%, and 9%) on growth were studied and GC-MS based metabolite profiling of A. montana hairy roots was conducted. The optimal growth and biomass accumulation of transformed roots were observed on an MS nutrient medium containing 3% or 5% sucrose. GC-MS analysis of hairy roots of A. montana showed the presence of 48 compounds in polar fractions and 22 compounds in apolar …
Effects Of Turkish Propolis Extract On Secretion Of Polymorphonuclear Elastase Following Respiratory Burst, Yaşam Barlak, Orhan Değer, Meltem Uçar, Tuğba Ni̇gar Çakiroğlu
Effects Of Turkish Propolis Extract On Secretion Of Polymorphonuclear Elastase Following Respiratory Burst, Yaşam Barlak, Orhan Değer, Meltem Uçar, Tuğba Ni̇gar Çakiroğlu
Turkish Journal of Biology
Propolis is a natural, resinous, hive product with various bioactive properties. Polymorphonuclear leukocytes (PMNLs) use both oxygen-dependent (respiratory burst) and oxygen-independent mechanisms (releasing proteolytic enzymes such as elastase) to destroy infectious agents. The in vitro effects of dimethyl sulfoxide (DMSO) extract of Turkish propolis on the secretion of PMN elastase from PMNLs following respiratory burst was studied. Phorbol myristate acetate was used to stimulate respiratory burst. The Flow Cytometric CellProbe Oxidative Burst Test was used to determinate if respiratory burst occurred or not and what its severity was after a 4 h incubation with propolis extracts (0 mg/mL, 0.625 mg/mL, …
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Turkish Journal of Biology
In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied. Rats weighing approximately 200-250 g were used in this experiment. They were divided into five groups, each one including five rats. Group I served as the normal control group while the others served as the experimental groups. The rats were exposed to oral administrations of lead acetate of 2.5 mg dissolved in 1 mL of drinking water per day. The administration lasted 96, 144, 336, and 432 h. The animals were anesthetized using chloroform inhalation and the peritoneum was stripped open and …
Phytofabrication Of Biomolecule-Coated Metallic Silver Nanoparticles Using Leaf Extracts Of In Vitro-Raised Bamboo Species And Its Anticancer Activity Against Human Pc3 Cell Lines, Kalamegam Kalaiarasi, Govindaraj Prasannaraj, Shivendra Vikram Sahi, Perumal Venkatachalam
Phytofabrication Of Biomolecule-Coated Metallic Silver Nanoparticles Using Leaf Extracts Of In Vitro-Raised Bamboo Species And Its Anticancer Activity Against Human Pc3 Cell Lines, Kalamegam Kalaiarasi, Govindaraj Prasannaraj, Shivendra Vikram Sahi, Perumal Venkatachalam
Turkish Journal of Biology
The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum (absorption peak at 365 nm), FTIR, TEM (spherical shape), SEM (size of 30-90 nm), and EDX analysis. Cell viability assays were carried …
An Improved Pvs2 Cryopreservation Technique For Ascocenda Wangsa Gold Orchid Using Protocorm-Like Bodies, Arulvilee Rajasegar, Asyraf Mansor, Ranjetta Poobathy, Elayabalan Sivalingam, Uma Rani Sinniah, Sreeramanan Subramaniam
An Improved Pvs2 Cryopreservation Technique For Ascocenda Wangsa Gold Orchid Using Protocorm-Like Bodies, Arulvilee Rajasegar, Asyraf Mansor, Ranjetta Poobathy, Elayabalan Sivalingam, Uma Rani Sinniah, Sreeramanan Subramaniam
Turkish Journal of Biology
Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate …
Enhancing The Expression Of Aspergillus Niger Ss-Mannanase In Pichia Pastoris By Coexpression Of Protein Disulfide Isomerase, Xiaoling Chen, Bo Zhou, Meng Xu, Zhiqing Huang, Gang Jia, Jiayun Qiao, Guangmang Liu
Enhancing The Expression Of Aspergillus Niger Ss-Mannanase In Pichia Pastoris By Coexpression Of Protein Disulfide Isomerase, Xiaoling Chen, Bo Zhou, Meng Xu, Zhiqing Huang, Gang Jia, Jiayun Qiao, Guangmang Liu
Turkish Journal of Biology
A gene encoding ß-mannanase from Aspergillus niger GIM3.452 was amplified and inserted into a pPIC9K vector. The resulting recombinant plasmid, pPIC9K-MAN, was transformed into Pichia pastoris GS115. One strain (GSKM-1) having the highest ß-mannanase activity of 26.6 U/mL was obtained. In order to increase the secretion of ß-mannanase in P. pastoris, we constructed a double recombinant yeast and made it coexpress protein disulfide isomerase. One strain (GSKZ\alphaM2) with the highest ß-mannanase activity of 40 U/mL was then obtained and used to optimize expression conditions. When the GSKZ\alphaM2 strain was induced under the optimized conditions (methanol concentration 1.5%, induction time 7 …
Artichoke Compound Cynarin Differentially Affects The Survival, Growth, And Stress Response Of Normal, Immortalized, And Cancerous Human Cells, Ceren Gezer, Sevi̇nç Yücecan, Suresh Inder Singh Rattan
Artichoke Compound Cynarin Differentially Affects The Survival, Growth, And Stress Response Of Normal, Immortalized, And Cancerous Human Cells, Ceren Gezer, Sevi̇nç Yücecan, Suresh Inder Singh Rattan
Turkish Journal of Biology
Cynarin (CYN) is the main derivative of caffeoylquinic acid, found in leaves and heads of artichoke. It may have hepatoprotective, antiatherosclerotic, antioxidative, choleretic, and cholesterol-lowering effects. We tested the effects of various doses of CYN on the proliferative potential, survival, morphology, and stress response (SR) markers heme oxygenase-1 (HO-1) and heat shock protein-70 (HSP70) in normal human skin fibroblasts (FSF-1), telomerase-immortalized mesenchymal stem cells (hTERT-MSC), and cervical cancer cells (HeLa). The effects of CYN on cell proliferation and morphology were dose- and cell type-dependent, with 500 µM CYN as the upper limit for all cell types. While the growth and …