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Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher

Turkish Journal of Biology

Background/aim: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of Chelidonium majus L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.

Materials and methods: In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, …

Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang

Turkish Journal of Biology

Background: Abdominal aortic aneurysm (AAA), a gradual segmental dilatation of the abdominal aorta, is associated with a high mortality rate. The pathophysiological molecular mechanisms underlying AAA remain unclear. In recent years, changes in miRNA levels have been reported to be involved in the development and treatment of AAA. This study aimed to investigate the potential targets and underlying mechanisms of miR-9-5p in attenuating AAA progression by modulating the inflammatory response.

Materials and methods: Biochemical kits were used to measure the levels of inflammatory factors, antioxidant enzyme activity, and serum oxidative stress in normal and AAA model mice. miR-9-5p overexpression was …

Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang

Turkish Journal of Biology

Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.

Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 10⁹ CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.

Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …

Biochemical And Proteomic Analyses Explain The Role Of Thiourea In Improving Tolerance Against Cadmium Toxicity In Maize, Mustafa Yildiz, Hakan Terzi̇, Nurgül Sönmez

Turkish Journal of Botany

Thiourea (TU), a sulfur-containing compound, is recognized for its ability to ameliorate the deleterious impacts of metal ions on plant growth. Nonetheless, the molecular mechanisms by which TU regulates metabolic activities in maize (Zea mays L.) under cadmium (Cd) stress remain largely unexplored. This study seeks to clarify how TU (35 μM) mitigates or enhances tolerance to Cd toxicity (50 μM) through physiological and proteomic methods. Cd exposure resulted in inhibited plant growth, leaf chlorosis, and lipid peroxidation; however, the addition of TU reduced Cd toxicity by decreasing Cd accumulation and modulating antioxidant enzyme activity. The global proteome profile was …

Effects Of Dietary Supplementation With Codium Fragile And Fennel Essential Oil On Growth, Enzyme Activity, And Hepatopancreas Histology In Juvenile Narrow-Clawed Crayfish (Pontastacus Leptodactylus Eschscholtz, 1823), Yavuz Mazlum, Meti̇n Yazici, Şehri̇ban Çek, Mehmet Naz, Musa Türkmen, Oğuz Yaşar Uzunmehmetoğlu, Kemal Dede

Turkish Journal of Zoology

This study investigated the impact of different levels of Codium fragile macroalgae (CF) dietary supplementation combined with a fixed concentration of 0.05% fennel (Foeniculum vulgare Mill. var. dulce Mill.) essential oil (FO) on the growth performance, enzyme activity, and hepatopancreas histological changes of juvenile narrow-clawed crayfish Pontastacus leptodactylus (initial average weight = 0.25 ± 0.04 g). A 90-day feeding trial was conducted using four experimental groups: CF + FO (control), 1% CF + FO, 2% CF + FO, and 4% CF + FO. The essential oil yield obtained from the fennel seeds was 2.4%. Anethole was the most abundant component …

Insights Into Therapeutic Discovery Through The Kelch Domain Structure Of Keap1 At Ambient Temperature, Merve Yilmaz, Belgi̇n Sever, Yi̇ği̇t Kutlu, Mehmet Gül, Ceren Okuducu, Serra Tavli, Masami Otsuka, Mikako Fujita, Turkan Hali̇loğlu, Hali̇li̇brahi̇m Çi̇ftçi̇, Hasan Demi̇rci̇

Turkish Journal of Biology

Background/aim: The Kelch-like-ECH associated protein 1 (Keap1) is an integral component of the E3-ubiquitin ligase complex, which binds to Nuclear factor erythroid 2-related factor 2 (Nrf2) and facilitates its degradation by the 26S proteasome. The Kelch domain of Keap1, composed of six repeated structural motifs, plays a key role in this interaction. This study aims to investigate the dimeric structure of the Keap1 Kelch domain at ambient temperature and to examine its implications for conformational dynamics, particularly in relation to the DMF and Nrf2 binding sites.Materials and methods: The dimeric crystal structure of the Keap1 Kelch domain was determined at …

Discovery And Characterization Of Selective Lipase-Inhibiting Polyheterocyclic Derivatives: A Combined In Silico And In Vitro Study, Madjda Benguechoua, Mebarka Imane Benguechoua, Khedidja Benarous, Houssem Boulebd, Ibtissem Kadi, Ari̇f Mermer, Yakup Şi̇ri̇n, Alaeddine Kaouka, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: Obesity has become a global health crisis with an increasing prevalence, necessitating the search for effective therapies. Schiff base derivatives, known for their broad pharmacological activities, have gained attention as potential antiobesity agents. This study aimed to investigate the lipase inhibitory potential of novel Schiff base derivatives and assess their drug-like properties through in vitro assays and in silico methods. Materials and methods: The lipase inhibitory activity of synthesized Schiff base derivatives was evaluated using in vitro assays, with IC50 values determined for each compound. Additionally, in silico ADMET predictions (absorption, distribution, metabolism, excretion, and toxicity), molecular docking studies, …

Comprehensive Characterization Of Purified Naringinase: Effect On Naringin Content, Antioxidant Activity, Phenolic Profile, And Grapefruit Juice Quality, Arzu İmece, Memnune Şengül, Bülent Çeti̇n, Ömer İrfan Küfrevi̇oğlu, Arzu Öztürk Kesebi̇r

Turkish Journal of Agriculture and Forestry

Bitterness arising from naringin is a problem in citrus juices, especially grapefruit juice, in terms of consumer preference. Naringinase enzyme is used to eliminate the bitterness. In this study, naringinase was extracellularly extracted, isolated, and characterized from an Aspergillus niger strain in different media. The extracted enzyme was added to grapefruit juice at different concentrations (0.1%, 0.5%, 1%) (0.078, 0.39 and 0.78 EU) and stored at different temperatures (25, 35, and 45 °C) for 5 h. At the end of the first and fifth h, the amount of naringin, physicochemical properties, phenolic profiles, and antioxidant activities of the grapefruit juice …

Antiinflammatory Mechanisms Of Barley-Based Functional Foods Beyond Diabetes Management, Hafiz Ghulam Muhu Din Ahmed, Adnan Noor Shah, Muhammad Ahtasham Mushtaq, Xiaomeng Yang, Jiazhen Yang, Xia Li, Li’E Yang, Xiaoying Pu, Yawen Zeng

Turkish Journal of Agriculture and Forestry

This review article investigated the antiinflammatory properties of barley, emphasizing its benefits beyond diabetes management. Barley, historically valued for its nutritional and medicinal properties, is rich in bioactive compounds such as beta-glucans, phenolic acids, and flavonoids. These compounds are known for their significant antioxidant and antiinflammatory activities. This study highlights the multifaceted mechanisms through which barley reduces systemic inflammation, including the enhancement of gut microbiota composition, the production of beneficial short-chain fatty acids, and the modulation of immune responses. Beta-glucans in barley lower cholesterol, and regulate glucose levels, while phenolic acids and flavonoids inhibit proinflammatory enzymes and cytokines, thereby reducing …

Effects Of Commiphora Myrrha Resin Extract/Nanosilver On Immune Cells, Pathogen, And Breast/Colon Cancer Cell Line Growth, Mohammad Y. Alshahrani, Essam H. Ibrahim, Abeer S. Alahmari, Mona Kilany, Ramadan Taha, Haitham I. El-Mekkawy, Amr Ahmed El-Arabey, Ahmed Ezzat Ahmed

Turkish Journal of Agriculture and Forestry

Commiphora myrrha grows in various places of the world and is used for many purposes. The objectives of this study were to examine the biological influences of C. myrrha resin extract (MRE) alone or with its biogenic nanosilver on microbial, immune, and cancer cell growth. C. myrrha resin was boiled to prepare the MRE. Functional groups in the MRE were explored using Fourier-transform infrared spectroscopy (FTIR). MRE was used to synthesize nanosilver that was characterized using an ultraviolet-visible (UV-Vis)-spectrophotometer and X-ray diffraction. In vivo experiments included the untreated group (G1) and MRE-orally treated group (G2). Cancer cell lines, HCT-116 (colon) …

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.

Application Of Vermicompost Extract To Improve The Phytochemical Composition Of Berry Fruits, Jelena Tomić, Žaklina Karaklajić-Stajić, Marijana Pešaković, Svetlana M. Paunović, Boris Rilak, Maja Mikulic-Petkovsek, Metka Hudina, Robert Veberic, Nesi̇be Ebru Kafkas, Sezai̇ Erci̇şli̇, Jerneja Jakopic

Turkish Journal of Agriculture and Forestry

The growing market demand for environmentally friendly products for plant nutrition has led to the development of new fertilizers, particularly liquid compost-based products such as teas, extracts, and leachates. The aim of this research was to compare the effect of chemical fertilization (CF) and its combination with liquid fertilizer based on vermicompost and beneficial microorganisms (CF + VP) on the phytochemical profile of strawberry (Senga Sengana), blackberry (Čačanska Bestrna) and blueberry (Aurora). The results showed that CF + VP positively affected fruit weight (8.8 g), soluble solids (8.9°Bx), total sugars (65.5 g/kg FW), and the sum of all phenolic groups …

In Vitro Regeneration Potential Of Pistacia Integerrima And Pistacia Khinjuk Rootstocks: A Modeling Approach For Optimal Medium Formulation, Heydem Eki̇nci̇, Beki̇r Erol Ak

Turkish Journal of Agriculture and Forestry

Micropropagation involves the development of whole plants from small meristematic tissues by culturing them in vitro under aseptic conditions on a nutrient medium. However, identifying a single nutrient medium suitable for propagating Pistacia, a recalcitrant species, remains challenging. While some species exhibit good growth on Murashige and Skoog (MS) medium, Pistacia species generally yield insufficient results. To address this, a five-dimensional experimental design was developed using Design- Expert software (Stat-Ease Inc., Minneapolis, MN, USA). Thirty-three experimental databases were generated by the software using the modified I-optimal criterion. MS medium served as the control treatment. A response surface model (RSM) employing …

In Vivo Emergence And Seedling Growth Of Ornamental Pepper Seeds Under Diatomaceous Earth Treatments, Deni̇z Hazar

Turkish Journal of Agriculture and Forestry

Propagation by seeds is very useful in ornamental horticulture, especially for flowering plant production. Seed germination and seedling growth are the most sensitive plant developmental stages and, in nurseries, are under the influence of many biotic and abiotic stress factors. Diatomaceous earth is made from the fossilized remains of tiny aquatic organisms called diatoms, and diatomaceous earth as supplemental Si has been shown to be beneficial for plant growth, flowering, and nutrient content under both well-watered and water-stressed conditions. This study aimed to determine the effects of different methods and doses of Si-containing diatomite on in vivo seed emergence and …

A Comprehensive Review Of Fermentation Technologies In Food Processing: From Traditional To Cutting Edge, Pragati Singh, Niharika Arya, Shalini Sharma, Ankita Sharma, Prasad Rasane, Supriya Singh Gaur, Sawinder Kaur, Mahendra Gunjal, Hasan Kiliçgün, Jyoti Singh

Turkish Journal of Agriculture and Forestry

Fermentation technologies play crucial roles in food processing, offering various advantages, including enhanced flavor profiles, extended shelf life, and improved nutritional qualities. This paper provides a comprehensive overview on the major types of fermentation as well as effects of various factors such as substrate composition, temperature, pH, oxygen availability, and microbial strain selection on the process. Despite its widespread application, traditional fermentation processes face limitations related to yield optimization, contamination risks, and process consistency. Recent advancements have significantly enhanced fermentation efficiency, product quality, and scalability, ultimately expanding the applications beyond food and beverage production into pharmaceuticals, nutraceuticals, biofuels, and waste …

Assessment Of Phenolic Composition, Sugars, And Antioxidant Capacity In Turkish Fig (Ficus Carica L.) Cultivars, Ghassan Zahid, Haşi̇m Kelebek, Ayzi̇n Küden, Flavien Shimira, Yildiz Aka Kaçar

Turkish Journal of Agriculture and Forestry

Fig fruits (Ficus carica L.) have significant health benefits. Türkiye is the leading fig producer in the world. Despite their diversity and rich medicinal properties, the biochemical fingerprints and nutritional quality of local figs are poorly understood. This study aimed to analyze the peels and pulps of eight Turkish figs with varying colors for their total phenolic composition, sugars, and antioxidant capacity. Thirty-one phenolic compounds (PCs) were detected using HPLC-MS/MS. The dark-purple 01-1N-58 genotype had the highest amount of colored PCs in fruit peels and pulps (151.25 and 31.57 mg/100 g dw, respectively). Likewise, the maximum quantity of colorless PCs …

The Role Of The Hemoglobin-Albumin-Lymphocyte-Platelet (Halp) Score In Deep Neck Infections And Its Relationship With Clinical Parameters, Ayşe Seçi̇l Kayali Di̇nç, Nagi̇han Gülhan Yaşar, Agah Yeni̇çeri̇, Çeti̇n Şahutoğlu, İbrahi̇m Sevi̇m, Meli̇h Çayönü

Turkish Journal of Medical Sciences

Background/aim: Deep neck infections (DNI) are suppurative infections of the cervical spaces, which may lead to life-threatening complications. The HALP score is a laboratory parameter reflecting a patient’s nutritional and inflammatory status. The aim of this study was to compare the HALP scores of patients with DNI with those of a control group. A secondary objective was to investigate the potential relationship between the HALP score and clinical parameters, and to assess its role in determining disease severity.

Materials and methods: The study retrospectively included patients who were hospitalized and treated for DNI. Data were extracted from the hospital’s electronic …

Expert Opinion On Clinical Presentation, Diagnosis And Treatment Of Infantile Onset Pompe Disease: A Delphi Study In Türkiye, Eki̇n Özsaydi Aktaşoğlu, Asli İnci̇, Ridvan Murat Öktem, Gürsel Bi̇beroğlu, İlyas Okur, Fati̇h Süheyl Ezgü, Fi̇li̇z Başak Cengi̇z Ergi̇n, Abdurrahman Akgün, Nur Arslan, Hali̇l İbrahi̇m Aydin, Ayşe Ergül Bozaci, Mahmut Çoker, Fatma Tuba Emi̇noğlu, Meli̇ke Ersoy, Emi̇ne Göksoy, Banu Kadioğlu Yilmaz, Mehtap Kağnici, Mustafa Kiliç, Nesli̇han Önenli̇ Mungan, Burcu Öztürk Hişmi̇, Pembe Soylu Üstkoyuncu, Ayşegül Tokatli, Ayşe Çi̇ğdem Aktuğlu Zeybek, Berrak Bi̇lgi̇ner Gürbüz, Sevi̇l Dorum, Di̇lek Güneş, Çi̇ğdem Seher Kasapkara, Sebi̇le Kilavuz, Erdoğan Soyuçen, Peli̇n Teke Kisa, Özlem Ünal Uzun, Fatma Derya Bulut, Songül Gökay, Selen Has Özhan, Deni̇z Kor, Aynur Küçükcongar Yavaş, Fi̇gen Özçay, Yilmaz Yildiz, Harun Yildiz, Leyla Tümer

Turkish Journal of Medical Sciences

Background/aim: Pompe disease (acid maltase deficiency, glycogen storage disease type II, OMIM #606800) is an autosomal recessive disorder characterized by lysosomal acid-α-glucosidase deficiency. The infantile-onset type of the disease is mainly characterized by cardiomegaly, hypotonia, and a high mortality rate. This study aimed to create a national consensus about infantile-onset Pompe disease (IOPD) to raise awareness among clinicians and standardize diagnosis and treatment approaches in Türkiye.

Materials and methods: The Gazi University Division of Metabolic Diseases and Nutrition developed this expert opinion consensus and expanded it to include metabolism specialists across Türkiye. A systematic literature review was performed, and the …

Investigation Of The Relationship Between Triglyceride Glucose Index And Metabolic And Hematological Parameters In Obese Individuals, Özge Baş Aksu, Feri̇de Özkara, Rifat Emral, Volkan Genç, Demet Çorapçioğlu, Mustafa Şahi̇n

Turkish Journal of Medical Sciences

Background/aim: This research examined the association between the triglyceride-glucose (TyG) index and metabolic, hepatic, and hematologic variables in a population of severely obese subjects, with a focus on the assessment of glycemic control.

Materials and methods: This retrospective study included 315 adult patients with a body mass index (BMI) ≥ 35 kg/m² who were evaluated for bariatric surgery. Participants were grouped according to HbA1c levels: those with HbA1c ≥ 6.5% and those with HbA1c < 6.5%. TyG, atherogenic index of plasma (AIP), fibrosis-4 (FIB-4), aspartate aminotransferase-to-platelet ratio index (APRI), and hematological inflammation markers were assessed and compared between groups. To investigate associations, correlation and regression analyses were conducted.

Results: Individuals with HbA1c ≥ 6.5% had significantly higher TyG, AIP, APRI, and FIB-4 values than those with lower HbA1c. The TyG index showed a strong …

Health Impacts And Medical Assistance After Libyan Flood Disaster: Emergency Medical Teams’ Responses, Hakan Güner, Okan Maden, Muhammed Saltuk Deni̇z, Kerem Dost Bi̇lmez, Şükrü Yorulmaz, Mehmet Enes Gökler

Turkish Journal of Medical Sciences

Background/aim: Floods are the most frequent natural disasters and pose direct and indirect health risks, some well-documented and others poorly understood. Epidemiological studies help bridge these gaps and guide effective public health responses.In September 2023, Storm Daniel caused severe flooding in Libya, damaging infrastructure, including healthcare facilities. The disaster affected 250,000 people, displaced 48,000, and claimed 15,000 lives, making it the second deadliest natural disaster of the year. This study aims to assess the characteristics of flood-affected patients to improve disaster preparedness.

Materials and methods: This descriptive study examined disaster victims who visited three clinics established as part of an …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Molecular Phylogeny And Population Structure Of Eurygaster Integriceps (Hemiptera: Scutelleridae) In The Southeastern Anatolia Region Of Türkiye, Mehmet Sali̇h Özgökçe, Merve Doğaç, Hi̇lmi̇ Kara

Turkish Journal of Zoology

The aim of this study was to determine the identity and distribution of the sunn pest Eurygaster spp. and to quantify the population density in wheat fields across Southeastern Anatolia, Türkiye. A total of 309 samples were collected from 76 fields in 54 districts across all provinces within the study region, where wheat production is intensive. A combination of morphological and molecular techniques was employed to identify the collected specimens. Morphological identification was based on male genitalia, and PCR amplification of the COI mitochondrial gene region was used for DNA barcoding. Both morphological and molecular analyses confirmed that all collected …

Distribution Pattern And Abundance Dynamics Of Asian Openbill (Anastomus Oscitans) In Rice Field Throughout Peninsular Malaysia, Badrulhadza Amzah, Rosli Ramli, Noorhidayah Mamat

Turkish Journal of Zoology

The Asian Openbill (Anastomus oscitans) plays a crucial role in rice field ecosystems by preying on invasive apple snails (Pomacea spp.), potentially reducing the reliance on chemical molluscicides. This study investigated the distribution and abundance dynamics of the stork across major rice granaries in Peninsular Malaysia. Surveys were conducted at 111 sampling points within 30 localities across 11 states during five consecutive rice seasons (2022–2024), with bird counts obtained through binoculars and digital photography. Results indicate that storks are highly concentrated on the northern and central west coasts, where rice fields provide abundant foraging opportunities. In the northern regions (Perlis, …

Molecular Crosstalk Of Transcription Factors And Hormones In Drought Tolerance Genes In Plants, Cengi̇z Kaya

Turkish Journal of Botany

Water deficit is a critical challenge to global agricultural productivity and food security, which is exacerbated by climate change and increasing water scarcity. To withstand drought stress, plants employ a variety of molecular strategies driven by complex regulatory networks. This review examines the central role of transcription factors (TFs) as master regulators that activate drought responsive genes, which in turn mediate key adaptive processes such as osmoregulation, antioxidative defense, and membrane stability. A distinctive aspect of this review is its focus on the dynamic interplay between TFs and hormonal signaling pathways, including abscisic acid, ethylene, salicylic acid, and jasmonic acid. …

Natural Microbial Consortium Construction Strategy From Rhizospheric Agricultural Soils To Improve Wheat (Triticum Durum Desf.) Seedling Growth, Ni̇da Arslan, Mi̇ne Gül Şeker, Özlem Akkaya, Yelda Özden Çi̇ftçi̇

Turkish Journal of Botany

Microbial consortia of compatible microorganisms play a key role in enhancing agricultural sustainability by optimizing nutrient uptake by promoting plant growth more effectively. This study aimed to identify local isolates with the most effective plant growth-promoting (PGP) properties and to form a consortium of isolates showing the optimal synergistic effect between plant and bacterial components to improve the growth of wheat plants. For this purpose, 48 isolates identified from 145 isolates were screened for their PGP activities. According to the results, 16S rRNA gene sequencing was performed to identify the six isolates exhibiting the most promising activity. Different consortium groups …

Genome-Wide Association Of Gene Regions Controlling Selenium And Magnesium Accumulation In Walnuts (Juglans Regia L.), İrem Büyükalgan, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Botany

Walnut (Juglans regia L.) is one of the most economically important species. It is of great importance to the food industry due to its nutritional value, and high content of many beneficial components such as macro and microelements. In the current study, the aim was to determine markers of single nucleotide polymorphism (SNP) associated with the gene(s) controlling the accumulation of Selenium (Se) and Magnesium (Mg) in walnuts. Se and Mg concentrations were measured in 154 genotypes of Juglans regia in 2021 and 2022. A total of 16,473 SNP markers were used for association mapping to analyze the marker-trait associations. …

Resveratrol Modulates Triosephosphate Isomerase And Mineralization In Osteosarcoma Cells: Potential Target For Novel Therapeutic Strategies, Gonca Tuna, Si̇bel Çinar Asa, Eli̇f Ertürk, Yaren Yildiz, Ferda Ari

Turkish Journal of Biology

Background/aim: Osteosarcoma, a primary malignant bone tumor, is challenging to treat due to its aggressive nature and limited therapies. Resveratrol (RES), a natural polyphenol, has potential anticancer properties. Hence, we investigated RES’s impact on osteosarcoma cells, focusing on triosephosphate isomerase (TPI) and related mechanisms.

Materials and methods: RES was applied to osteosarcoma (SaOS-2) and healthy fetal osteoblast (hFOB 1.19) cells for 48 h, and cell viability was measured by SRB assay. The mode of cell death was examined using Hoechst 33342/annexin V/propidium iodide. TPI and methylglyoxal (MG) enzyme levels were determined by ELISA. The effect of RES on …

Prooxidant Effect Of Uric Acid On Human Leukocytic Dna: An In Vitro And Ex Vivo Study, Yim Tong Savio Szeto, Vincy Sze Wing Li, Yuen Lam Pon

Turkish Journal of Biology

Background/aim: Uric acid is a major contributor to the total antioxidant capacity of human plasma. However, this endogenous substance’s antioxidant and prooxidant properties have not yet been reported.

Conclusion: In this study, the comet assay was employed in vitro to determine the effect of uric acid on DNA damage in human lymphocytes and leukocytic DNA damage in hyperuricemia patients with and without renal failure.

Results: DNA damage in lymphocytes occurred at uric acid concentrations of ≥600 μM. Adding catalase to the uric acid solution diminished the damaging effect, indicating that hydrogen peroxide mediated the prooxidant activity. Moreover, …

Sustainable Forage Yield Improvement Through Perennial Grass–Legume Intercropping In Degraded Grasslands, Alina-Ștefania Stanciu

Turkish Journal of Agriculture and Forestry

Grasslands constitute one of the largest terrestrial biomes, providing essential ecosystem services such as biodiversity preservation, food production, and climate change mitigation. Globally, the desertification and degradation of grasslands have been accelerated by intensified land use and climate change. It is crucial to conserve and restore grasslands to address climate and biodiversity challenges. However, due to their diverse nature and undervalued benefits for humans, grasslands have often been overlooked in terms of preservation, conservation, and restoration compared to forests. In sustainable agriculture, intercropping grasses with legumes can enhance crop nutritional quality, increase productivity, and impact soil microbiological activity, ultimately leading …

Silage Yield And Quality Of Different Grass Pea (Lathyrus Sativus L.) Genotypes, Murat Azak, Erdem Gülümser

Turkish Journal of Agriculture and Forestry

This study aimed to determine the silage yield and quality of different 12 grass pea (Lathyrus sativus L.) genotypes (Gap Mavisi, İptaş, Karadağ, 1603, 2006, 2401, 4301, 4403, 5001, 6408, 6410, and S3). The trial was established in Bilecik’s ecological conditions in 2022 and 2023. It was carried out according to the randomized block design with four replications, and the plants were harvested at the full flowering stage. The harvested plants were chopped to 2 cm in size and ensiled in vacuum bags and left to ferment at 25 ± 2 °C for 45 days. The silage yield, pH, dry …