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Herniaria Caucasica (Caryophyllaceae), Rumex Cyprius (Polygonaceae), And Potentilla Multifida (Rosaceae); 3 New Records For Turkey, Murat Koç, Ergi̇n Hamzaoğlu
Herniaria Caucasica (Caryophyllaceae), Rumex Cyprius (Polygonaceae), And Potentilla Multifida (Rosaceae); 3 New Records For Turkey, Murat Koç, Ergi̇n Hamzaoğlu
Turkish Journal of Botany
Herniaria caucasica Rupr., Rumex cyprius Murb., and Potentilla multifida L. are given as new records for Turkey. The specimens were collected from Van, Mersin, and Kayseri provinces. These new records were compared with similar species in Turkey. Descriptions, distributions, and figures of these new records are given.
Flower Closure In The Field Bindweed (Convolvulus Arvensis): A Field Test Of The Pollination Hypothesis, Pavol Prokop, Dominika Neupauerova
Flower Closure In The Field Bindweed (Convolvulus Arvensis): A Field Test Of The Pollination Hypothesis, Pavol Prokop, Dominika Neupauerova
Turkish Journal of Botany
Although the behavioral plasticity of flower traits has received considerable attention, its adaptive value is not thoroughly understood. We experimentally examined flower opening/closure in field bindweed (Convolvulus arvensis), a species with short flower persistence (1 day) in which adaptive responses to pollination were not previously expected. In line with the pollination hypothesis we suggested that flower exposure in this species should respond to pollination. More specifically, we predicted that flower closure in the pollinated flowers would be quicker than that in unpollinated flowers. As predicted, the unpollinated flowers were open for a longer time than the pollinated flowers. There was …
Ellenberg’S Indicator Values For Soil Nitrogen Concentration And Ph In Selected Swamp Forests In The Central Black Sea Region Of Turkey, Burak Sürmen, Hamdi̇ Güray Kutbay, Dudu Duygu Kiliç, Rena Huseyinova, Mahmut Kilinç
Ellenberg’S Indicator Values For Soil Nitrogen Concentration And Ph In Selected Swamp Forests In The Central Black Sea Region Of Turkey, Burak Sürmen, Hamdi̇ Güray Kutbay, Dudu Duygu Kiliç, Rena Huseyinova, Mahmut Kilinç
Turkish Journal of Botany
Ellenberg developed a system that assigns indicator scores for habitat characteristics of individual plant species. Swamp forests are highly diverse ecological communities and include different plant species, all of which have different ecological requirements. Ellenberg's indicator values (EIVs) were calculated for soil pH and nitrogen (N) concentration in some swamp forest species in the Central Black Sea region of Turkey. In this study, the EIVs for soil pH were usually similar in the swamp forest species studied, while some differences were found with respect to EIVs for soil nitrogen concentration. Tolerance values (TVs) of all of the studied species were …
Phytochemical Importance Of Medicinal Plants As Potential Sources Of Anticancer Agents, Himani Raina, Garima Soni, Nupur Jauhari, Neelam Sharma, Navneeta Bharadvaja
Phytochemical Importance Of Medicinal Plants As Potential Sources Of Anticancer Agents, Himani Raina, Garima Soni, Nupur Jauhari, Neelam Sharma, Navneeta Bharadvaja
Turkish Journal of Botany
The diverse and magnificent plant kingdom of the world is widely known for its medicinal importance. The potential medicinal properties of plant species have contributed significantly in the development of various herbal therapies for a number of diseases across the globe. The benefits of herbal medicine over allopathic medicine have helped medicinal plants to regain their importance in the field of health and medicine. Cancer is one of the major health problems that have widely affected the world's population. There is a great need to combat this disease with better and more effective medication as compared to existing therapies. A …
Subcellular Localization And Developmental Regulation Of Cytosolic, Soluble Pyrophosphatase Isoforms In Arabidopsis Thaliana, Zahi̇de Nesli̇han Öztürk, Steffen Greiner, Thomas Rausch
Subcellular Localization And Developmental Regulation Of Cytosolic, Soluble Pyrophosphatase Isoforms In Arabidopsis Thaliana, Zahi̇de Nesli̇han Öztürk, Steffen Greiner, Thomas Rausch
Turkish Journal of Botany
Pyrophosphate (PP_{I}) is the by-product of several reversible key reactions of primary metabolism. Thus, generated PP_i should be removed by hydrolysis via pyrophosphatases to prevent accumulation and to drive anabolism. In plastids, a plastidic, soluble pyrophosphatase splits PP_i released by ADPG pyrophosphorylase. The cytosolic PP_i pool is believed to be hydrolyzed by tonoplast- and/or Golgi-integral H^{+}-translocating pyrophosphatases. The Arabidopsis thaliana (L.) Heynh. genome encodes 6 soluble pyrophosphatase isoforms (PPas), 1 of which was shown to be localized in plastids. The remaining 5 of those PPas (PPas 1-5) are more similar to each other with highly conserved protein sequences. They all …
Assessment Of Genetic Diversity And Phylogenetic Relationships Of Endangered Endemic Plant Barbarea Integrifolia Dc. (Brassicaceae) In Turkey, Ertuğrul Fi̇li̇z, Etem Osma, Ali̇ Kandemi̇r, Hüseyi̇n Tombuloğlu, Guzi̇n Tombuloglu, Seda Bi̇rbi̇lener, Mehtap Aydin
Assessment Of Genetic Diversity And Phylogenetic Relationships Of Endangered Endemic Plant Barbarea Integrifolia Dc. (Brassicaceae) In Turkey, Ertuğrul Fi̇li̇z, Etem Osma, Ali̇ Kandemi̇r, Hüseyi̇n Tombuloğlu, Guzi̇n Tombuloglu, Seda Bi̇rbi̇lener, Mehtap Aydin
Turkish Journal of Botany
Barbarea integrifolia DC. (Brassicaceae) is an endangered and endemic species located in Erzincan and Gümüşhane provinces of Turkey. In total, 27 individuals from 2 natural populations were assessed using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method coupled with sequence analysis of the internal transcribed spacer (ITS1) rDNA region. Genetic accuracy of RAPD-PCR was tested with 25 RAPD primers, and it resulted in 115 clear and reproducible DNA fragments from 13 RAPD primers. Among these, 76 (66.1%) fragments were found to be polymorphic. According to the ITS1 analysis of B. integrifolia populations, the plants exhibited a relatively poor …
Genotoxic Effects Of Zinc Oxide And Titanium Dioxide Nanoparticles On Root Meristem Cells Of Allium Cepa By Comet Assay, Eşref Demi̇r, Nuray Kaya, Bülent Kaya
Genotoxic Effects Of Zinc Oxide And Titanium Dioxide Nanoparticles On Root Meristem Cells Of Allium Cepa By Comet Assay, Eşref Demi̇r, Nuray Kaya, Bülent Kaya
Turkish Journal of Biology
Nanogenotoxicity is an emergent field, relevant for estimating the potential genotoxic risk of nanomaterials. In this study we investigated the genotoxic potential of zinc oxide (ZnO, Keywords: Zinc oxide nanoparticles, titanium dioxide nanoparticles, Allium cepa, comet assay, genotoxicity, DNA damage
Molecular Characterization Of The Encoding Regions And Tissue Expression Analyses For 3 Novel Buffalo Akt Genes, Akt1, Akt2, And Akt3, Chunfeng Wu, Lixian Liu, Jinlong Huo, Dalin Li, Yueyun Yuan, Feng Yuan, Yongwang Miao
Molecular Characterization Of The Encoding Regions And Tissue Expression Analyses For 3 Novel Buffalo Akt Genes, Akt1, Akt2, And Akt3, Chunfeng Wu, Lixian Liu, Jinlong Huo, Dalin Li, Yueyun Yuan, Feng Yuan, Yongwang Miao
Turkish Journal of Biology
The objective of this study was to obtain the complete coding sequences (CDSs) of 3 buffalo genes (AKT1, AKT2, and AKT3) using reverse-transcriptase polymerase chain reaction and to depict their molecular characterizations and tissue expression patterns in buffalo. The buffalo AKT1, AKT2, and AKT3 CDSs were 1443 bp, 1446 bp, and 1440 bp in length and encoded 480, 481, and 479 amino acids, respectively. Nine, 13, and 3 nucleotide differences were found in the CDSs between buffalo and other bovine species. Phylogenetic analyses showed that buffalo AKT1 and AKT2 have close genetic relationships with other species in the family Bovidae, …
Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi
Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi
Turkish Journal of Biology
Beta-galactosidases (\BETAgals) (EC 3.2.1.23) have been detected in a wide range of plant organs and tissues and are described by their ability to hydrolyze terminal nonreducing \BETA-D-galactosyl residues from \BETA-D-galactosides. In this study, 92 \BETAgal protein sequences from different plants, 7 animal samples including human and mouse, 3 samples from bacteria including Escherichia coli, and 4 samples from insects including Drosophila melanogaster were aligned. Sequences were analyzed by computational tools to predict the protein properties, such as molecular mass, isoelectric point, signal peptide, motifs, transmembrane domain, and secondary and spatial structure. Protein structure analysis revealed there is a high identity …
Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ
Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The aim of this investigation was to determine, purify, and characterize an antibacterial substance produced by Serratia marcescens Mm3 (Sm-Mm3) isolated from a common pest of hazelnut in Turkey, Melolontha melolontha, which is also a significant pest all over the world. A highly active antibacterial substance produced by Sm-Mm3 was purified by ammonium sulfate precipitation and high-performance liquid chromatography, respectively, and identified by spectrometric analysis (TOF-MS). Finally, we showed that this molecule is heat-stable, stable in the pH range of 5-9, and insensitive to organic solvents and proteinase K. The molecular mass was determined as 479 Da. Plasmid elimination demonstrated …
Serum Leptin Profiles, Food Intake, And Body Weight In Melatonin-Implanted Syrian Hamsters (Mesocricetus Auratus) Exposed To Long And Short Photoperiods, Bülent Gündüz
Turkish Journal of Biology
We have shown that circadian changes in leptin concentrations are inversely linked to circulating melatonin levels in the Syrian hamster. The present study aimed to assess the effects of subcutaneous melatonin implants under different photoperiodic conditions: did the implants affect serum leptin levels, body weight, food consumption, and/or testicular weight? Male hamsters were exposed to long and short photoperiods for 10 weeks and received subcutaneous melatonin implants (1 mg in 24 mg beeswax) every 2 weeks. Blood samples were collected every week at midday (1200 hours) or midnight (0000 hours) to assess leptin and melatonin concentrations. Testes were measured to …
Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Nematodes are the principal animal parasites of plants, causing annual crop losses of more than US$100 billion worldwide. Conventional control measures against nematode infection include toxic nematicide application to soil, crop rotation practices, and classical breeding approaches. However, due to the limitations of each technique, biotechnology presents itself as an effective alternative in nematode control. To date, several resistance genes against nematodes have been cloned. One such gene, Hs1^{pro1}, cloned from wild sugar beet, Beta procumbens, shows resistance to the cyst nematode Heterodera schachtii. Recent studies showed that Hs1^{pro1} has a broad target pathogen range. In this study, we transformed …
Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz
Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz
Turkish Journal of Biology
The aim of this study was to introduce antimicrobial activity to stainless steel orthodontic arch wires by coating them with TiO_2 in anatase form. Stainless steel (0.016 × 0.022 inch), D-rect (0.016 × 0.022 inch), and multistranded hammered retainer wires (0.014 × 0.018 inch) were coated with TiO_2 anatase by the sol-gel dip coating method. The wires were assessed for their photocatalytic antimicrobial activity against Streptococcus mutans, Candida albicans, and Enterococcus faecalis. After illumination under UVA (315-400 nm) at 1.0 mW/cm^2 for 1 h, the reduction efficiencies of the anatase-coated arch wires were calculated by using colony-forming unit counts. All …
Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao
Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao
Turkish Journal of Biology
Akirin2 is a recently discovered gene related to immune responses that also plays an important role in skeletal myogenesis. In this study, in order to scale up the production of recombinant porcine Akirin2 (pAkirin2), we report the expression and purification of a His-tagged version of recombinant pAkirin2 in Escherichia coli. The pAkirin2 is a polypeptide of 203 amino acids containing 42 rare codons. The recombinant pAkirin2 was expressed as an N-terminal fusion protein with His6 tag in E. coli Rosetta (DE3) host strain. The protein was purified by Ni-IDA affinity chromatography, yielding over 90% highly purified recombinant pAkirin2, with a …
Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu
Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu
Turkish Journal of Biology
The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosome-specific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform method. PCR products were separated by capillary electrophoresis and marker genotypes were determined. A total of 41 different alleles were observed. The mean allele number was 5.86 and mean F_{IS} value was 0.427 for all populations. The INRA189 locus was monomorphic for all populations except for South Anatolian Red. UMN0307 …
Hepatoprotective Effects Of Malva Sylvestris L. Against Paracetamol-Induced Hepatotoxicity, Liaqat Hussain, Javaria Ikram, Kanwal Rehman, Muhammad Tariq, Muhammad Ibrahim, Muhammad Sajid Hamid Akash
Hepatoprotective Effects Of Malva Sylvestris L. Against Paracetamol-Induced Hepatotoxicity, Liaqat Hussain, Javaria Ikram, Kanwal Rehman, Muhammad Tariq, Muhammad Ibrahim, Muhammad Sajid Hamid Akash
Turkish Journal of Biology
Malva sylvestris is traditionally used for the treatment of liver diseases, but sufficient pharmacological-based scientific literature is not available online to authenticate its use in liver ailments. We aimed to assess the hepatoprotective effects of Malva sylvestris against paracetamol-induced hepatotoxicity in mice. The extract was concentrated using a rotary evaporator and then desired concentrations of extracts were made by dissolving in normal saline. The standard drug silymarin (100 mg/kg) was used as a reference drug to compare the therapeutic effects of Malva sylvestris. Two different doses of Malva sylvestris (300 and 600 mg/kg) were administered intraperitoneally for 7 consecutive days …
Development Of Durable Antimicrobial Surfaces Containing Silver- And Zinc-Ion-Exchanged Zeolites, Zeynep İyi̇gündoğdu, Selami̇ Demi̇rci̇, Nurcan Baç, Fi̇kretti̇n Şahi̇n
Development Of Durable Antimicrobial Surfaces Containing Silver- And Zinc-Ion-Exchanged Zeolites, Zeynep İyi̇gündoğdu, Selami̇ Demi̇rci̇, Nurcan Baç, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
The present work involves development of stable antimicrobial materials containing silver- and zinc-ion-exchanged zeolites. Faujasite X and Linde type A zeolites were synthesized, and following ion exchange with Ag^+ and Zn^{++} ions they were found to exhibit antimicrobial effects against bacteria (E. coli, P. aeruginosa, and S. aureus), yeast (C. albicans and C. glabrata), and fungi (A. niger and P. expansum). Zeolites-X and -A containing silver and zinc ions were then mixed with various coating materials, including paints and polypropylene, to develop antimicrobial composites. The long-term antimicrobial characteristics of zeolite-containing composite materials were investigated by inoculating selected microorganisms onto the …
Peripheral Administration Of Kisspeptin Antagonist Does Not Alter Basal Plasma Testosterone But Decreases Plasma Adiponectin Levels In Adult Male Rhesus Macaques, Tanzeel Huma, Farhad Ulla, Farnaz Hanif, Joshua D. Rizak, Muhammad Shahab
Peripheral Administration Of Kisspeptin Antagonist Does Not Alter Basal Plasma Testosterone But Decreases Plasma Adiponectin Levels In Adult Male Rhesus Macaques, Tanzeel Huma, Farhad Ulla, Farnaz Hanif, Joshua D. Rizak, Muhammad Shahab
Turkish Journal of Biology
Kisspeptin signaling is a potent regulator of the reproductive axis and an elicitor of gonadotropin-releasing hormone secretion. The present study assessed the effect of the peripheral administration of a kisspeptin antagonist (P234) on testosterone secretion in the adult male rhesus monkey. Five monkeys were administered with either P234 (38.8 µg/kg body weight) or a vehicle (0.9% saline). Plasma testosterone and adiponectin levels, as a positive control, were then measured using specific ELISA assays from blood samples collected sequentially at 15-min intervals from 60 min prior to until 360 min after the P234/vehicle injection. In 3 monkeys, the experiment was repeated …
Preconditioning Effect Of Cytokinins On In Vitro Multiplication Of Embryonic Node Of Grass Pea (Lathyrus Sativus L.) Cultivar Gürbüz, Surendra Barpete, Muhammad Aasim, Khalid Mahmood Khawar, Sancar Fati̇h Özcan, Sebahatti̇n Özcan
Preconditioning Effect Of Cytokinins On In Vitro Multiplication Of Embryonic Node Of Grass Pea (Lathyrus Sativus L.) Cultivar Gürbüz, Surendra Barpete, Muhammad Aasim, Khalid Mahmood Khawar, Sancar Fati̇h Özcan, Sebahatti̇n Özcan
Turkish Journal of Biology
Lathyrus sativus L. (grass pea) is an important grain legume crop grown all over the world. It is very recalcitrant and difficult to regenerate under in vitro conditions. The present study determined the effect of preconditioning of cytokinins [10 and 20 mg L^{-1} thidiazuron (TDZ) and 2-isopentenyl adenine (2iP)] and postconditioning of dissimilar levels of TDZ-indole-3-butyric acid (IBA) and 2iP-IBA on in vitro shoot multiplication using embryonic node explants. The results showed that preconditioning followed by postconditioning on Murashige and Skoog (MS) medium with 0.25, 0.50, 0.75, and 1.0 mg L^{-1} 2iP or TDZ combined with 0.10 mg L^{-1} IBA …
Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş
Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş
Turkish Journal of Biology
In the present study, we tried to investigate the effects of melatonin, a novel antioxidant and a potent free radical scavenger, in stress-induced cerebral, cerebellar, cardiac, and hepatic oxidative damage using microscopic and biochemical analysis. A total of 32 male Wistar albino rats were divided into control, stress, stress + saline, and stress + melatonin groups. The rats from the stress groups were exposed to high stress conditions of starvation, immobilization, and cold exposure. The rats from the stress + melatonin group received melatonin daily at 20 mg/kg body weight intraperitoneally for 7 days. At the end of the experiment, …
Styrian Oil Pumpkin Pollen Germinability At Higher Irradiation Doses: Optimization Of The In Vitro Germination Protocol And Irradiation Procedure, Kristina Kosmrlj, Damijana Kastelec, Borut Bohanec
Styrian Oil Pumpkin Pollen Germinability At Higher Irradiation Doses: Optimization Of The In Vitro Germination Protocol And Irradiation Procedure, Kristina Kosmrlj, Damijana Kastelec, Borut Bohanec
Turkish Journal of Biology
Protocols for haploid induction in cucurbits are based on pollination with irradiated pollen, but the induction frequency is low and the majority of obtained embryos are zygotic. The longevity of Cucurbita pepo L. pollen is short even under natural conditions; following irradiation, germinability is decreased even further. This study was initiated to develop an optimal in vitro germination medium for styrian oil pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) pollen, which would enable accurate germination testing. Different pH values and the addition of sucrose, mannitol, and polyethylene glycol to the Brewbaker and Kwack germination medium were tested. The …
Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin
Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin
Turkish Journal of Biology
In human and animal organisms, lead can cause reproductive problems beginning with pregnant females. The reproductive axis is particularly sensitive to lead, its influence resulting in a delayed sexual maturity due to biosynthesis suppression of the sexual steroids. An animal model study was carried out on 28 white Wistar adult female rats, divided into 3 experimental (E) groups that were exposed for 12 months to lead acetate in drinking water as follows: 50 ppb Pb (E_1), 100 ppb Pb (E_2), and 150 ppb Pb (E_3), with a control group (M) that received unleaded tap water. Levels of FSH, LH, estradiol, …
Variation In Soil C And Microbial Functions Across Tree Canopy Projection And Open Grassland Microenvironments, Roberto Lai, Alessandra Lagomarsino, Luigi Ledda, Pier Paolo Roggero
Variation In Soil C And Microbial Functions Across Tree Canopy Projection And Open Grassland Microenvironments, Roberto Lai, Alessandra Lagomarsino, Luigi Ledda, Pier Paolo Roggero
Turkish Journal of Agriculture and Forestry
Mixtures of grasslands and perennial woody crops or vineyards represent a major source of potential carbon storage or release. Understanding the spatial variability of soil properties in these ecosystems is important in determining soil constraints related to the management of soil resources. The aims of the study were 1) to explore the spatial variability associated to the trees for soil C storage and its components and reactivity; and 2) to assess the similarities between microenvironments in terms of microbial functional diversity. Eight microenvironments characterized by different long-term soil management practices and different positions with respect to woody plant canopy soil …