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Articles 1981 - 2010 of 8894

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A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li Jan 2019

A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li

Turkish Journal of Biology

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.


Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru Jan 2019

Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru

Turkish Journal of Biology

Diclofenac sodium (DS), a potent inhibitor of cyclooxygenase, reduces the release of arachidonic acid and formation of prostaglandins. Being a nonsteroid drug that shows antiinflammatory action, the possible side effects of fetal DS administration gain importance in public and medical applications. Herein, the effects of DS administration (1 mg/kg) during gestational days 5-20 were investigated on the performance of Wistar rat pups in a variety of behavioral tasks. Four-week-old pups were subjected to sensory motor tests, a plus maze, an open field, the Morris water maze, and a radial arm maze. Fetal DS disrupted some sensory motor performances, such as …


A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ Jan 2019

A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

This study reports a new Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Heliothis peltigera (Denis & Schiffermuller), collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size. The genome of HearNPV-TR was digested with KpnI and XhoI enzymes and calculated as 130.5 kb. Phylogenetic analysis showed that HearNPV-TR has close relation with …


In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran Jan 2019

In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran

Turkish Journal of Biology

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity …


Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz Jan 2019

Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz

Turkish Journal of Biology

Camphor is widely used in pharmacy, the food industry, and cosmetics. In this study, we evaluate inhibitory and cytotoxic effects of camphor in the fission yeast (Schizosaccharomyces pombe), which presents a unicellular model in mechanistic toxicology and cell biology. Low-dose camphor exposure (0.4 mg/mL) activated autophagy, which was shown by GFP-Atg8 dots and transcriptional upregulation of Atg6 (Beclin-1 ortholog). Autophagy was also confirmed by using autophagy-deficient cells, which showed reduction in GFP-Atg8 dot formation. However, high-dose camphor exposure (0.8 mg/mL) caused dramatic cell death ratios, demonstrated by spot and colony-forming assays, even in autophagy-deficient cells. To unravel the underlying mechanism, …


Identification Of Streptomyces Strains Isolated From Humulus Lupulus Rhizosphere And Determination Of Plant Growth Promotion Potential Of Selected Strains, Fadi̇me Özdemi̇r Koçak Jan 2019

Identification Of Streptomyces Strains Isolated From Humulus Lupulus Rhizosphere And Determination Of Plant Growth Promotion Potential Of Selected Strains, Fadi̇me Özdemi̇r Koçak

Turkish Journal of Biology

In the present study it was aimed to obtain novel strains of actinomycetes that have the ability to promote plant growth. For this, seven soil samples from the rhizosphere of Humulus lupulus (Pazaryeri, Bilecik) were used and potential isolates were obtained. 16S rRNA genes of 30 isolates were amplified by PCR and sequenced. Eighteen isolates were found to be closely related to Streptomycetes spp. and they were tested for their proteolytic activity, cellulase activity, phosphate solubility, IAA production, biofilm formation, and growth in nitrogen-limited medium. Two isolates, HCI 12 and HCI 36, were selected according to the results of these …


Chicken Feather Protein Hydrolysate As A Biostimulant Improves The Growth Of Wheat Seedlings By Affecting Biochemical And Physiological Parameters, Ebru Genç, Ökkeş Atici Jan 2019

Chicken Feather Protein Hydrolysate As A Biostimulant Improves The Growth Of Wheat Seedlings By Affecting Biochemical And Physiological Parameters, Ebru Genç, Ökkeş Atici

Turkish Journal of Botany

We derived a protein hydrolysate with rich content, which includes proteinogenic and nonproteinogenic amino acids, from chicken feathers by using an alkaline hydrolysis procedure with high yield success. After foliar application of aqueous solutions (0.05%, 0.075%, and 0.1%) of chicken feather protein hydrolysate (CFPH) to the seedlings of wheat (Triticum aestivum L. `Altındane? and `Bezostaya?), their effects were evaluated on important physiological and biochemical parameters. Treating plants with CFPH stimulated the expression of RuBisCo and increased root and shoot lengths, fresh and dry weights of the seedlings, and photosynthetic pigment content. In addition, applying CFPH at 0.075% and 0.1% concentrations …


Contributions To The Genus Riccia L. (Ricciaceae) In Turkey, Hati̇ce Özenoğlu, Mesut Kirmaci, Ferhat Ki̇remi̇t Jan 2019

Contributions To The Genus Riccia L. (Ricciaceae) In Turkey, Hati̇ce Özenoğlu, Mesut Kirmaci, Ferhat Ki̇remi̇t

Turkish Journal of Botany

This study provides an identification key to the species of Riccia in Turkey and also gives information about detailed morphological and anatomic characters, reproductive forms, detailed photos of Riccia species and their spores, ecological preferences, and distribution patterns in the Mediterranean, Europe, and Southwest Asia. The number of Riccia taxa in Turkey has reached 27 including R. atromarginata, reported for the first time from Turkey in this study. Additionally, the presence of R. gougetiana var. armatissima and R. trabutiana in Turkey is confirmed here. This study reports other distribution areas of the species R. bicarinata, R. ciliata, R. ciliifera, R. …


Genome Size Variation Among Natural Populations Of Brachypodium Distachyon And B. Hybridum Collected From Different Regions Of Turkey, Gülsemi̇n Savaş Tuna, İsmet Başer, Meti̇n Tuna Jan 2019

Genome Size Variation Among Natural Populations Of Brachypodium Distachyon And B. Hybridum Collected From Different Regions Of Turkey, Gülsemi̇n Savaş Tuna, İsmet Başer, Meti̇n Tuna

Turkish Journal of Botany

Brachypodium distachyon (L.) P.Beauv. is a model grass species that is useful for studying temperate cereal, forage, and energy crops. In this study we aimed to determine the genome size and species identity in the collections of the B. distachyon complex that included samples from 56 different locations in Turkey, applying chromosome counting and nuclear genome size evaluation by flow cytometry method (FCM). Moreover, the study examined whether diverse geographical conditions at the collecting sites influence the genome size. Chromosomal analysis revealed that the number of B. distachyon (x = 5, 2n = 10) genotypes was 48 and the number …


Forage Pea (Pisum Sativum Var. Arvense L.) Landraces Reveal Morphological Andgenetic Diversities, Gürkan Demi̇rkol, Nuri̇ Yilmaz Jan 2019

Forage Pea (Pisum Sativum Var. Arvense L.) Landraces Reveal Morphological Andgenetic Diversities, Gürkan Demi̇rkol, Nuri̇ Yilmaz

Turkish Journal of Botany

The objective of this study was to assess morphological and genetic diversities of 48 forage pea landraces collected from different locations at different altitudes in Turkey. Morphological, quality, and yield features were determined for the landraces and three control cultivars in three subsequent years. Genetic diversities of the landraces and cultivars were also monitored using microsatellite (SSR) markers. Our results revealed that the features of landraces are significantly different. The hay weights and the relative feed values were found to be significantly affected by altitude, with the landraces generally showing significantly higher hay weight and relative feed values at lower …


Syntaxonomic Analysis Of Forests, Shrubs, And Steppes Of Tavşan Mountain(Amasya, Turkey), Cengi̇z Yildirim, Erkan Yalçin, Arzu Cansaran, Hasan Korkmaz Jan 2019

Syntaxonomic Analysis Of Forests, Shrubs, And Steppes Of Tavşan Mountain(Amasya, Turkey), Cengi̇z Yildirim, Erkan Yalçin, Arzu Cansaran, Hasan Korkmaz

Turkish Journal of Botany

The aim of this study is to reveal the syntaxonomical structure of Tavşan Mountain in the Euxinian transition area in northern Turkey. Eight plant associations were determined and classified using the Braun?Blanquet phytosociological approach. Detrended correspondence analysis was also used to reveal the environmental heterogeneity among the plant associations. Raunkiaer life form ratios of syntaxa were calculated. Shannon?Wiener index values also were determined for each plant association. The results of this study suggested that which environmental factors could be decisive in community diversity and syntaxonomical status.


Antinociceptive Effect Of Liposomal Bupivacaine Formulations After Intrathecal Administration In Rats, İlker Yi̇ği̇t, Berri̇n Günaydin, Ömer Hakan Emmez, Meci̇t Orhan Uludağ, Nur Banu Bal, İsmai̇l Tuncer Deği̇m Jan 2019

Antinociceptive Effect Of Liposomal Bupivacaine Formulations After Intrathecal Administration In Rats, İlker Yi̇ği̇t, Berri̇n Günaydin, Ömer Hakan Emmez, Meci̇t Orhan Uludağ, Nur Banu Bal, İsmai̇l Tuncer Deği̇m

Turkish Journal of Medical Sciences

Background/aim: Based on our previous in vitro study with multilamellar liposomal bupivacaine (MLB) versus bupivacaine alone in artificial cerebrospinal fluid, we aimed to investigate in vivo antinociceptive effect of intrathecal MLB by determining tail flick latency (TFL) time after thermal stimulation in rats. Materials and methods: After preparing MLB and high-yield drug entrapment in liposome (HYDEL) bupivacaine, 18 female Wistar rats were assigned to 3 groups as control (bupivacaine) and study groups (MLB and HYDEL bupivacaine) including 6 rats in each group to administer these drugs intrathecally. Antinociceptive activity was determined in terms of TFL time after thermal stimulation. Maximum …


Irak-4 Rs4251481 Gene Variant: As A Risk Factor On Inflammatory Bowel Disease, Gonca Candan, Resul Kahraman, Hayri̇ye Arzu Ergen Jan 2019

Irak-4 Rs4251481 Gene Variant: As A Risk Factor On Inflammatory Bowel Disease, Gonca Candan, Resul Kahraman, Hayri̇ye Arzu Ergen

Turkish Journal of Medical Sciences

Background/aim: Abnormal immune response occurs in individuals who have alleles associated with innate and adaptive immune mechanisms that predispose to inflammatory bowel disease (IBD). Interleukin-1 receptor-associated kinase 4 (IRAK-4) involved in the pathway produces cytokines that initiate and maintain inflammation through Toll-like receptors and interleukin-1 receptors on the membranes of innate immune cells are stimulated with antigens. It was aimed to investigate whether IRAK-4 rs3794262 and rs4251481 polymorphisms predispose to IBD and the possible effects of these polymorphisms by examining these gene polymorphisms with the clinic and prognostic parameters of IBD.Material and methods: Real-time PCR technique was used to detect …


Mid-Dose Losartan Mitigates Diabetes-Induced Hepatic Damage By Regulating Inos, Enos, Vegf, And Nf-?B Expressions, Fati̇h Oltulu, Ayli̇n Buhur, Çevi̇k Gürel, Gökçe Ceren Kuşçu, Meli̇h Dağdevi̇ren, Nefi̇se Ülkü Karabay Yavaşoğlu, Ti̇mur Köse, Altuğ Yavaşoğlu Jan 2019

Mid-Dose Losartan Mitigates Diabetes-Induced Hepatic Damage By Regulating Inos, Enos, Vegf, And Nf-?B Expressions, Fati̇h Oltulu, Ayli̇n Buhur, Çevi̇k Gürel, Gökçe Ceren Kuşçu, Meli̇h Dağdevi̇ren, Nefi̇se Ülkü Karabay Yavaşoğlu, Ti̇mur Köse, Altuğ Yavaşoğlu

Turkish Journal of Medical Sciences

Background/aim: Losartan, an antihypertensive drug, is highly preferred in patients with diabetes mellitus (DM) and hypertension because of its retarding effect on diabetic nephropathy. In this study, we investigated the potential therapeutic effect of different doses of losartan on hepatic damage in a streptozotocin (STZ, 50 mg/kg)-induced DM model in rats.Materials and methods: In this study, five different groups were formed: control, DM, low-dose losartan (5 mg/kg), mid-dose losartan (20 mg/kg), and high-dose losartan (80 mg/kg). Liver tissues of experimental groups were evaluated immunohistochemically for TUNEL, iNOS, eNOS, VEGF, and NF-?B pathways. In addition to immunohistochemical analysis, analyses of SOD …


Blue Sky At Midnight - Earthquake Lightning, Neil Whitehead, Ülkü Ulusoy Jan 2019

Blue Sky At Midnight - Earthquake Lightning, Neil Whitehead, Ülkü Ulusoy

Turkish Journal of Earth Sciences

Earthquake light, emerging from the ground as flashes at night (neglecting other more minor forms), usually has a white hemispherical center and blue outer part. The blue resembles daytime blue sky. Its existence is increasingly verified, with about 80 videos on the web to mid-2017. However, the light must be differentiated from power-grid faults, so sound/color/form/length criteria were developed in this paper through examination of many videos. Light should be coseismic (i.e. when the S-waves arrive), hemispherical, not during a storm, less than 1 s long, without fire or smoke, and without many rapid color changes. Warnings about camera artifacts …


Assessment Of Low Doses Of Eimeria Tenella Sporulated Oocysts On The Biochemical Parameters And Intestinal Microflora Of Chickens, Iva Langrová, Darina Chodova, Eva Tumova, Barbora Horakova, Romana Krejcirova, Martina Saskova, Roman Svejstil, Ivana Jankovska, Jaroslav Vadlejch, Zuzana Cadkova, Ivana Knizkova, Stanislava Nechybova Jan 2019

Assessment Of Low Doses Of Eimeria Tenella Sporulated Oocysts On The Biochemical Parameters And Intestinal Microflora Of Chickens, Iva Langrová, Darina Chodova, Eva Tumova, Barbora Horakova, Romana Krejcirova, Martina Saskova, Roman Svejstil, Ivana Jankovska, Jaroslav Vadlejch, Zuzana Cadkova, Ivana Knizkova, Stanislava Nechybova

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to evaluate the biochemical parameters and intestinal microflora in broilers with low doses of Eimeria tenella. Birds from the experimental groups were orally infected with 500 (GI) or 1000 (GII) sporulated oocysts of E. tenella and then examined for weight gain, biochemical indicators, and microbiota on day 9 and 16 after infection. Experimental infection of E. tenella on day 9 post infection (pi) increased concentrations of uric acid, cholesterol, triacylglycerols, and glucose; however, these measurements did not differ from those of the control group on day 16 pi. On day 16 pi, bifidobacteria counts were …


Chemical, Enzymatic, And Antioxidant Enrichments Of Full-Fat Soybean And Sunflower Meal By Bacillus Subtilis (Atcc$^{®}$ 6633$^{Tm}$) Fermentation Using A Solid-State Bioreactor, Yaşar Karakurt, Damla Güverci̇n, Sercan Önder, Ci̇van Çeli̇k, Ramazan Tosun, Burak Baran, Sulhatti̇n Yaşar Jan 2019

Chemical, Enzymatic, And Antioxidant Enrichments Of Full-Fat Soybean And Sunflower Meal By Bacillus Subtilis (Atcc$^{®}$ 6633$^{Tm}$) Fermentation Using A Solid-State Bioreactor, Yaşar Karakurt, Damla Güverci̇n, Sercan Önder, Ci̇van Çeli̇k, Ramazan Tosun, Burak Baran, Sulhatti̇n Yaşar

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to fortify nutritional and chemical composition of sunflower meal (SFM) and full-fat soybean (FFSB) used as a protein source in farm animal nutrition by Bacillus subtilis ATCC 6633 fermentation. An optimized set of fermentation parameters (60% moisture, 6.5-7.0 pH, 30-35 °C temperature, continuous aeration of 0.5-1.0 L/min and agitation of 20-100 rpm) were used to ferment SFM and FFSB samples with or without using bacterial inoculant for 0, 24, 48, and 72 h. The results indicated that the total organic acids (TOA) contents and crude ash (CA) of SFM and FFSB increased significantly, but there were …


Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina Jan 2019

Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to evaluate the in vivo efficacy of pumpkin seeds as an alternative natural anthelmintic for chickens. Ninety Philippine Jolo native chickens of mixed sexes, aged 4?5 months and weighing 1?2 kg, were randomly distributed into three treatment groups with 30 chickens per group. Control group A was fed basic mash feed, group B received feed mixed with ground pumpkin seeds (2 g/bird per day), and group C received mebendazole-medicated feed (30 mg/kg body weight). Fifteen randomly selected chickens from each group were euthanized and necropsied before treatment, and the remaining fifteen in each group were …


A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi Jan 2019

A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi

Turkish Journal of Veterinary & Animal Sciences

Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …


Management Of Dystocia Due To Unilateral Uterine Torsion In A Labrador Bitch: A Surgical Approach, Sameer Ali Mohammed, Kavitha Kannan, Arunmozhi Narayanasamy, Sankar Palanisamy, Sridevi Purushothaman Jan 2019

Management Of Dystocia Due To Unilateral Uterine Torsion In A Labrador Bitch: A Surgical Approach, Sameer Ali Mohammed, Kavitha Kannan, Arunmozhi Narayanasamy, Sankar Palanisamy, Sridevi Purushothaman

Turkish Journal of Veterinary & Animal Sciences

Uterine torsion is an uncommon disorder of bitch and is more likely to occur in the pregnant animals. A three-year-old Labrador bitch was presented to the Madras Veterinary College Teaching Hospital with the history of having delivered two live puppies in the morning with no further signs of parturition. On abdominal palpation, the fetal mass was palpable. Radiography revealed three fetal skeletons and ultrasound revealed viable fetuses. Based on the history and clinical examination, the case was tentatively diagnosed as primary uterine inertia. Even after two inductions, no puppies were delivered and hence to save the life of puppies, emergency …


Old Age-Associated Impairment Of The Rat Liver Antioxidant Defense System: The Basis For Affirmation Of The Experimental Model, Marko Miler, Vladimir Ajdzanovic, Jasmina Zivanovic, Ivana Jaric, Branka Sosic-Jurjevic, Milica Potrebic, Verica Milosevic Jan 2019

Old Age-Associated Impairment Of The Rat Liver Antioxidant Defense System: The Basis For Affirmation Of The Experimental Model, Marko Miler, Vladimir Ajdzanovic, Jasmina Zivanovic, Ivana Jaric, Branka Sosic-Jurjevic, Milica Potrebic, Verica Milosevic

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.


Sybr Green-Based Real-Time Pcr Detection Of Canine Babesia Spp. In Ixodid Ticks Infesting Dogs In Kerala, South India, Lavinia Wahlang, Bindu Lakshmanan, Naicy Thomas, Anu Bosewell, Jain Jose, Sunanda Chulliyil, Aravindakshan Thazhathuveetil Jan 2019

Sybr Green-Based Real-Time Pcr Detection Of Canine Babesia Spp. In Ixodid Ticks Infesting Dogs In Kerala, South India, Lavinia Wahlang, Bindu Lakshmanan, Naicy Thomas, Anu Bosewell, Jain Jose, Sunanda Chulliyil, Aravindakshan Thazhathuveetil

Turkish Journal of Veterinary & Animal Sciences

Ticks, being hematophagous ectoparasites, are notorious vectors that transmit an array of pathogenic viruses, bacteria, and protozoa while simultaneously exhibiting a zoonotic potential by transmitting pathogens that affect the health of owners in contact. The distribution of ixodid ticks of the genera Rhipicephalus and Haemaphysalis spp. in tropical climate of the state contributes to many serious tick-borne parasitic and rickettsial infections in domestic and wild canines. In south India, canine babesiosis is one of the most prevalent vector-borne parasitic diseases. Detection of parasites in tick vectors has significant epidemiological implications. The present study was designed to identify the presence of …


Determination Of Antioxidants In Bovine Oviduct Epithelial Cell Culture Isolated At Different Periods Of The Estrous Cycle, Aytül Kürüm, Si̇yami̇ Karahan, Hakan Kocamiş, Mi̇yase Çinar, Emel Ergün Jan 2019

Determination Of Antioxidants In Bovine Oviduct Epithelial Cell Culture Isolated At Different Periods Of The Estrous Cycle, Aytül Kürüm, Si̇yami̇ Karahan, Hakan Kocamiş, Mi̇yase Çinar, Emel Ergün

Turkish Journal of Veterinary & Animal Sciences

Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the …


A Preliminary Study On Effects Of Subanesthetic Doses Of Preemptive Ketamine Given Prior To Premedication On Total Intravenous Anesthesia For Short- To Medium-Term Surgical Procedures In Horses, Pallvi Sharma, Aswathy Gopinathan, Kiranjeet Singh, Sherin B. Sarangom, Chelladurai Sowbharenya, Christina John, Med Ram Verma Jan 2019

A Preliminary Study On Effects Of Subanesthetic Doses Of Preemptive Ketamine Given Prior To Premedication On Total Intravenous Anesthesia For Short- To Medium-Term Surgical Procedures In Horses, Pallvi Sharma, Aswathy Gopinathan, Kiranjeet Singh, Sherin B. Sarangom, Chelladurai Sowbharenya, Christina John, Med Ram Verma

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effects of subanesthetic doses of ketamine given prior to premedication on the quality of anesthesia, recovery, and postoperative pain in horses. Eighteen horses were randomly recruited into three equal groups, S, LK, and HK, wherein saline, ketamine at 0.2 mg/kg body weight (bwt), and ketamine at 0.4 mg/kg bwt were given, respectively, i.v. for 30 min as a continuous rate infusion (CRI). Horses were premedicated with xylazine at 1 mg/kg bwt i.v. and butorphanol at 0.05 mg/kg bwt i.v. after 30 min. Anesthesia was induced using ketamine and midazolam and was maintained with …


A Possible Correlation Between Diet, Serum Oxytetracycline Concentration, And Onset Of Reproductive Disturbances In Bitches: Clinical Observations And Preliminary Results, Alessandro Di Cerbo, Gianandrea Guidetti, Sergio Canello, Raffaella Cocco Jan 2019

A Possible Correlation Between Diet, Serum Oxytetracycline Concentration, And Onset Of Reproductive Disturbances In Bitches: Clinical Observations And Preliminary Results, Alessandro Di Cerbo, Gianandrea Guidetti, Sergio Canello, Raffaella Cocco

Turkish Journal of Veterinary & Animal Sciences

Based on the increase of food-related disturbances in animals and the recent findings concerning the toxicity of oxytetracycline (OTC) presence within pet food, we compared the effectiveness of two commercially available diets in 36 bitches suffering from reproductive disturbances. We ran a randomized clinical evaluation over a 90-day period. Bitches were randomly assigned to receive a control diet (CD) or a nutraceutical diet (ND) and were assessed for the presence of OTC in their sera before and at the end of the study. Sixteen of 18 bitches in the ND group went into heat within an average of 35.5 ± …


Phylogenetic Analyses Of P32, Rpo30, Gpcr, Orf117, And Kelch-Like Genes Of Tunisian Sheeppox Virus Isolates, Jihene Lachheb, Aicha Turki, Houssem Mastour, Jihene Nsiri, Imen El Behi, Imen Labri, Abdeljelil Ghram Jan 2019

Phylogenetic Analyses Of P32, Rpo30, Gpcr, Orf117, And Kelch-Like Genes Of Tunisian Sheeppox Virus Isolates, Jihene Lachheb, Aicha Turki, Houssem Mastour, Jihene Nsiri, Imen El Behi, Imen Labri, Abdeljelil Ghram

Turkish Journal of Veterinary & Animal Sciences

Sheeppox is an economically important disease of small ruminants caused by the sheeppox virus (SPPV). Sheeppox virus (SPPV), goatpox virus, and lumpy skin disease virus are capripoxviruses, antigenically related but genetically distinct. Their differentiation requires analysis at a molecular level. Eleven sheeppox virus isolates from five different regions of Tunisia were collected between 2010 and 2016. SPPV was detected by real-time PCR using SYBR Green technology. Primers amplifying the entire Kelch-like gene were designed. Then the GPCR, RPO30, P32, ORF117, and Kelch-like genes were molecularly analyzed to show the different specific lineage patterns of each species. Phylogenetic analyses revealed three …


Impact Of Light-Emitting Diode And Compact Fluorescent Light Source Type And Cage Tier On Layers Reared In An Enriched Cage System Part 1: Production Performance And Egg Quality, Gülsüm Tünaydin, Bi̇lgehan Yilmaz Di̇kmen Jan 2019

Impact Of Light-Emitting Diode And Compact Fluorescent Light Source Type And Cage Tier On Layers Reared In An Enriched Cage System Part 1: Production Performance And Egg Quality, Gülsüm Tünaydin, Bi̇lgehan Yilmaz Di̇kmen

Turkish Journal of Veterinary & Animal Sciences

This study?s aim was to investigate the effects of age, light source type (light-emitting diode (LED), and compact fluorescent (FLO)), and cage tier on production performance and egg quality traits of layers reared in the enriched cage system. A total of 800 Nick Chick White layers were used in the study. A total of 800 eggs were used for egg quality traits at 25 and 45 weeks of age. The enriched cage tiers were coded as I, II, III, and IV from bottom to top. Layer age significantly affected egg mass, hen-day egg production rate, hen-house egg production rate, feed …


Evaluation Of The Correlation Between Quantitative Measurement Of The Foveal Avascular Zone And Retinal Vessel Density And Outer Retinal Disruptions In Diabetic Patients, Nagi̇han Uğurlu, Ayşe Güzi̇n Taşlipinar Uzel, Ahmet Şengün, Fatma Yülek, Demet Özdaş, Abbas Ali̇ Tam, Reyhan Ersoy, Beki̇r Çakir Jan 2019

Evaluation Of The Correlation Between Quantitative Measurement Of The Foveal Avascular Zone And Retinal Vessel Density And Outer Retinal Disruptions In Diabetic Patients, Nagi̇han Uğurlu, Ayşe Güzi̇n Taşlipinar Uzel, Ahmet Şengün, Fatma Yülek, Demet Özdaş, Abbas Ali̇ Tam, Reyhan Ersoy, Beki̇r Çakir

Turkish Journal of Medical Sciences

Background/aim: The aim of the current study was to evaluate the correlation between the integrity of the outer retinal layers on optical coherence tomography (OCT) and objective parameters of retinal microvascular perfusion on optical coherence tomography angiography (OCTA).Materials and methods: A total of 105 eyes of 54 diabetic patients were included in the study. Integrity of the outer retinal layers including the external limiting membrane (ELM), ellipsoid zone (EZ), and interdigitation zone (IZ) was assessed by spectral-domain optical coherence tomography. The foveal avascular zone (FAZ) area and vessel density (VD) measurements in the superficial capillary plexus (SCP) and deep capillary …


Protective Effect Of Cyclosporine A In The Treatment Of Severe Hydronephrosis In A Rabbit Renal Pelvic Perfusion Model, Ben Zheng Zhou, Da Hu Zhang, Wei Min Yu, Jin Zhuo Ning Jan 2019

Protective Effect Of Cyclosporine A In The Treatment Of Severe Hydronephrosis In A Rabbit Renal Pelvic Perfusion Model, Ben Zheng Zhou, Da Hu Zhang, Wei Min Yu, Jin Zhuo Ning

Turkish Journal of Medical Sciences

Background/aim: Cyclosporine A (CsA), a traditional immunosuppressive compound, has been reported to specifically prevent isch-emia reperfusion tissue injury via apoptosis pathway. This study aimed to explore the renoprotective effects of CsA on the kidneys of rabbits undergoing renal pelvic perfusion.Materials and methods: A total of 30 rabbits were randomly assigned into a control group (n = 6) and an experimental group (n = 24). The experimental group underwent a surgical procedure that induced severe hydronephrosis and was then stochastically divided into 4 groups (S1, S1?, S2, and S2?), consisting of 6 rabbits each. Groups S1 and S1? were perfused with …


Thiamin-Induced Variations In Oxidative Defense Processes In White Clover(Trifolium Repens L.) Under Water Deficit Stress, Adeel Ghaffar, Nudrat Aisha Akram, Muhammad Ashraf, Yasin Ashraf, Muhammad Sadiq Jan 2019

Thiamin-Induced Variations In Oxidative Defense Processes In White Clover(Trifolium Repens L.) Under Water Deficit Stress, Adeel Ghaffar, Nudrat Aisha Akram, Muhammad Ashraf, Yasin Ashraf, Muhammad Sadiq

Turkish Journal of Botany

Vitamins are regarded as vital growth substances. They act as cofactors of various key enzymes involved in metabolism and also have vital influence on metabolic activities of plants. They are believed to play a significant role in plants against abiotic stresses, particularly during water stress. The current experiment was conducted to evaluate the role of exogenously applied thiamin on two white clover cultivars, Super Late Fsd and Layalpur Late, at varying [100% (control), 80%, and 60% field capacities] water regimes. A significant suppression was noted in plant biomass, plant height, chlorophyll a and b contents, total soluble proteins, and shoot …