Digital Commons Network^™

The Enhancement Of Crispr/Cas9 Gene Editing Using Metformin, Jaedyn L. Rollins, Raquel M. Hall, Clara J. Lemus, Lauren A. Leisten, Jennifer M. Johnston

Faculty Research, Scholarly, and Creative Activity

The CRISPR/Cas9 technology is a revolutionary tool that can be used to edit the genome. Specifically, the genome of hematopoietic stem cells (HSCs) could be edited to correct monogenic blood disorders as well as produce immunotherapies. However, the efficiency of editing HSCs remains low. To overcome this hurdle, we set out to investigate the use of metformin, an FDA-approved drug, to enhance gene modification. We assessed the effect of metformin on the growth of two hematopoietic cell lines: a myeloid-erythroid leukemic cell line (K562 cells) representative of the myeloid population and an immortalized T lymphocyte cell line (Jurkat cells) representative …

Static Management Presents A Simple Solution To A Dynamic Fishery And Conservation Challenge, Christopher M. Free, Lyall F. Bellquist, Karin A. Forney, Jenn Humberstone, Kate Kauer, Qi Lee, Owen R. Liu, Jameal F. Samhouri, Jono R. Wilson, Darcy Bradley

Faculty Research, Scholarly, and Creative Activity

Dynamic ocean management, which leverages near real-time data to adaptively shift management in response to changing ocean conditions, is gaining attention as an alternative to static approaches for managing dynamic fisheries challenges. While promising, dynamic management can be data-intensive, costly, and difficult to implement, and its value relative to simpler static approaches should be evaluated before being applied, especially when endangered species and economically crucial fisheries are at risk. Here, we use management strategy evaluation to compare static and dynamic management strategies for reducing humpback whale (Megaptera novaeangliae) entanglement risk in the highly lucrative California Dungeness crab (Metacarcinus magister) trap …

The Effect Of Histone Deacetylase Inhibitors On The Efficiency Of The Crispr/Cas9 System, Ymer Björnson, Codey Y. Huang, Jaedyn L. Rollins, Guadalupe Castañeda, Navneet Kaur, Emiko Yamamoto, Jennifer M. Johnston

Faculty Research, Scholarly, and Creative Activity

The CRISPR/Cas9 technology is a prominent genome-editing tool capable of producing a double-strand break in the genome. However, the modification of hematopoietic stem cells via the homology-directed repair pathway is still inefficient. Therefore, we hypothesize that histone deacetylase inhibitors, such as valproic acid (VPA) and sodium butyrate (NaB), could enhance HDR efficiency by increasing the accessibility of the genome-editing machinery. To address the potential utilization of HDAC inhibitors therapeutically, we began by assessing the effect of VPA and NaB on two cell lines representative of the two hematopoietic stem cell lineages. No statistically significant effect on cell growth or viability …

Perspectives On The Doctor Of Public Health (Drph) Education Among Students And Alumni In The United States: A Cross-Sectional National Online Survey, Chulwoo Park, Cindy Delgado, Ans Irfan

Faculty Research, Scholarly, and Creative Activity

Background

This study explored the current and desired identity of the DrPH degree, focusing on whether the competencies set by the Council on Education for Public Health (CEPH) adequately prepare DrPH graduates for effective public health practice. Additionally, the study investigated the necessity of standardization in DrPH training, referring to a consensus-driven approach that equips future public health practitioners with practical skillsets applicable in real-world scenarios.

Methods

A national cross-sectional online survey titled “National DrPH leaders & practitioners needs assessment” was conducted from November 2020 to February 2021. The survey was based on a self-report by DrPH students and DrPH …

Metaversity And Metaworth, Two Important Prerequisites To Prepare For The Metaverse, Mohammad Fathi, Ahmad Shabani, Alireza Mansouri

Library Philosophy and Practice (e-journal)

Currently, the world is in the fourth industrial revolution, where the speed of innovation has accelerated both in terms of development and diffusion; technologies bring more in sync with the needs of societies, and the complex technologies of a new world named “Metaverse” are rapidly increasing, expanding, and spreading. There are many uncertainties and questions in this emerging. This paper, after trying to understand the metaverse, its opportunities, and challenges, has discussed the reasons for the necessity to prepare societies, how to make policies, and finally how to enter this mysterious world. After studying the sources and information available in …

Review, Democracy And Fake News: Information Manipulation And Post-Truth Politics, Peter Krapp

Secrecy and Society

No abstract provided.

Records Management Practices In Government Ministries In Akwa Ibom State, Nigeria, Udoh Isaac Unwana, Angela Okeoghene Bello, Osaze Patrick Ijiekhuamhen

Library Philosophy and Practice (e-journal)

This study was on a survey of records management practices in state ministries in Akwa Ibom State, Nigeria. The survey design was used for the study, with the questionnaire as the instrument for data collection. The population of the study was one hundred and six (106) records management staff of the ministries studied. Two research questions were posed for investigation. The study revealed that various records are created by the ministries which include records on general administration, accounts and finance, sports, health, laws, education and environments. The study also revealed that the ministries used such facilities as wooden shelves, metallic …

A Field Assessment Of Residential Laundry To Landscape Greywater Quality In The San Francisco Bay Area, Sara Khosrowshahi Asl, Katherine Cushing, Rachel O'Malley, Alexandra Dahl, Afshin Rouhani, Sherry Bryan, Justin Burks

Faculty Research, Scholarly, and Creative Activity

Potable water scarcity is a global issue. Recent and ongoing droughts in the Western United States make residential water conservation crucial. Several water agencies have invested in conservation programs that educate residents on non-traditional water sources, such as laundry-to-landscape (L2L) greywater systems, which reuse washing machine water for outdoor irrigation. This study analyzed landscape vegetation and 21 greywater characteristics of 30 households with L2L systems in Santa Clara County, California. Greywater from most residential systems, even ones decades-old and unmaintained, had acceptable reuse values for major water quality parameters tested. Overall, 89% of fecal coliform counts fell within the acceptable …

Multi-Dimensional Surface Water Quality Analyses In The Manawatu River Catchment, New Zealand, Imokhai T. Tenebe, Jason P. Julian, Praise God C. Emenike, Nathaniel Dede-Bamfo, Omeje Maxwell, Samuel E. Sanni, Eunice O. Babatunde, Darlan D. Alves

Faculty Research, Scholarly, and Creative Activity

Land Use and Land Cover (LULC) properties give vital information about pollution signatures in rivers, and they help develop best management practices (BMPs) for effective water resource management. This work employs multivariate statistical methods, receptor modeling, connectivity analysis, and univariate trend analysis to investigate pollution sources across spatiotemporal scales in the Manawatu River, New Zealand. A positive matrix factorization (PMF) method was applied to interpret possible contamination sources. A 25-year dataset (1989–2014) comprising 12 water quality variables from three sites was used. Runoff connectivity analyses identified high-producing grassland (HG) as the most dominant pollution class in all sub-catchments. Univariate analyses …

Abyssal Hydrothermal Springs-Cryptic Incubators For Brooding Octopus, James P. Barry, Steven Y. Litvin, Andrew Devogelaere, David W. Caress, Chris F. Lovera, Amanda S. Kahn, Erica J. Burton, Chad King, Jennifer B. Paduan, C. Geoffrey Wheat, Fanny Girard, Sebastian Sudek, Anne M. Hartwell, Alana D. Sherman, Paul R. Mcgill, Aaron Schnittger, Janet R. Voight, Eric J. Martin

Faculty Research, Scholarly, and Creative Activity

Does warmth from hydrothermal springs play a vital role in the biology and ecology of abyssal animals? Deep off central California, thousands of octopus (Muusoctopus robustus) migrate through cold dark waters to hydrothermal springs near an extinct volcano to mate, nest, and die, forming the largest known aggregation of octopus on Earth. Warmth from the springs plays a key role by raising metabolic rates, speeding embryonic development, and presumably increasing reproductive success; we show that brood times for females are ∼1.8 years, far faster than expected for abyssal octopods. Using a high-resolution subsea mapping system, we created landscapescale maps and …

A Cost-Effective And Smart Sensing Tissue-Like Testbed For Surgical Training, Lysette Zaragoza

McNair Research Journal SJSU

A low-cost tissue-like testbed with six nodes of varying stiffness was developed for surgical training to provide pressure and force feedback data through image reception to human operators. Using SolidWorks, a 3D model of the box trainer housing was created. A pad for the distribution of smartsensing nodes and microcontroller connections was designed with open spaces for the respective components. The pad was 3D-printed with PLA filament. Flat piezoelectric pressure sensors were fabricated with conductive materials and velostat sensor material. Using static and dynamic analyses, three top sensors were chosen to be used in three pressure sensing nodes. A calibration …

Understanding Our Airways: How Lung Structure And Function Is Impacted By Respiratory Infections And Immune Responses, Katherine Coll

McNair Research Journal SJSU

Understanding the underlying functions and interplaying systems that make up the respiratory system is a crucial step in the research and development of treatments for respiratory illnesses. In this literature review, I explore the complex biochemical processes that occur in the pulmonary epithelium and endothelium. As epithelial and endothelial cells serve a multitude of functions such as host protection and nutrient regulation, discussing the interplay between these cells and intercellular junctions and their immediate impact on the respiratory system is essential to understanding the impact of respiratory dysfunctions and diseases. This review further examines the different types of intercellular junctions …

The Impact Of Culture On Health Perceptions And Help-Seeking Behaviors Among Older Latinos With Co-Occurring Diabetes And Depression: A Literature Review, Alejandra M. Aguirre

McNair Research Journal SJSU

Diabetes is one of the most common health disparities in the United States today and disproportionately affects older Latino populations. Increased recognition of the bidirectional relationship between diabetes and mental health has led to more awareness and treatments for diabetes and depression. Despite high prevalence rates, diabetes and depression are commonly undiagnosed and untreated in older Latinos. Existing literature reports that factors including genetic and behavioral factors, low socioeconomic status (SES), limited healthcare access, and language barriers may prevent this population from seeking professional treatment. Prior research also suggests that culturally based stigma toward these conditions can negatively impact health …

Front Matter, Maria Cruz

McNair Research Journal SJSU

No abstract provided.

Full Issue

McNair Research Journal SJSU

No abstract provided.

Research Data Management Practices And Challenges In Academic Libraries: A Comprehensive Review, Subaveerapandiyan A

Library Philosophy and Practice (e-journal)

This comprehensive review article delves into the current landscape of research data management (RDM) practices and challenges faced by academic libraries across various regions. Utilizing a wide range of studies and data collected from different countries, this article aims to provide a comprehensive overview of the state of RDM services, the role of librarians, and the advancements in technology within academic libraries. The review explores the importance of RDM in supporting open science, data sharing, and reproducibility while also shedding light on areas that require further development and improvement.

Causality, Agency, And Moral Responsibility In Nikaya Buddhism, Soo Lam Wong

Comparative Philosophy

In this paper, I aim to examine the relationship between the Buddhist notions of causality and agency, the questions of whether the Buddhist notion of causality affirms causal determinism and whether the Buddhist notion of agency affirms libertarian free will, the implications of the Buddhist notions of causality and agency for moral responsibility, and the implications of the Buddhist rejection of the metaphysical self for agency and moral responsibility. My claim is that although the question of whether the early Buddhist notions of causality and agency affirm causal determinism and libertarian free will respectively remains open, they are compatible with …

Daoist Freedom, Psychological Hygiene, And Social Criticism, Yun Tang

Comparative Philosophy

The article explores the inner logic and defining features of Daoist freedom. It argues that Daoist freedom can be meaningfully understood as psychological hygiene, and it suggests that Daoist xuan-jie (懸解) can be rendered possible only if one can rid oneself of intensional suffering—an idea ultimately inspired by Friedrich Nietzsche. This comparative approach enables the article contribute to the received way of understanding Daoist freedom by stressing its dialectics: by being at ease with one’s social and political environment, Daoist freedom demands social criticism, calling for room for people to be able to act spontaneously so that …

The Dangers Of Re-Colonization: Possible Boundaries Between Latin American Philosophy And Indigenous Philosophy From Latin America, Jorge Sanchez-Perez

Comparative Philosophy

The field of Latin American philosophy has established itself as a relevant subfield of philosophical inquiry. However, there might be good reasons to consider that our focus on the subfield could have distracted us from considering another subfield that, although it might share some geographical proximity, does not share the same historical basic elements. In this paper, I argue for a possible and meaningful conceptual difference between Latin American Philosophy and Indigenous philosophy produced in Latin America. First, I raise what I call Mariátegui’s Solidarity Challenge to show that there might be some neglectful treatment of the philosophical views of …

Analyzing Nietzsche And Darwin In Search Of Origin Of Morality: The Evolving Perspective, Anirban Ghosh, Malabika Chakrabarti

Comparative Philosophy

It is generally believed that the greatest asset of human being is the moral values and according to theist such values have been infused in human by the creator. By accepting such view we simply get rid of any effort of searching the origin of morality or ethics and also transfer the responsibility of being ethical on the almighty. But when atheist denied God, the liability of being moral comes to human and also the significant question arose why we should be moral. Probably more important is the hunt for the origin of our morality. In this article we have …

Global Assessment Of Marine Plastic Exposure Risk For Oceanic Birds, Bethany L. Clark, Ana P.B. Carneiro, Elizabeth J. Pearmain, Marie Morgane Rouyer, Thomas A. Clay, Win Cowger, Richard A. Phillips, Andrea Manica, Carolina Hazin, Marcus Eriksen, Jacob González-Solís, Josh Adams, Yuri V. Albores-Barajas, Joanna Alfaro-Shigueto, Maria Saldanha Alho, Deusa Teixeira Araujo, José Manuel Arcos, Fiona Mcduie, For Full Author List, See Comments Below

Faculty Research, Scholarly, and Creative Activity

Plastic pollution is distributed patchily around the world's oceans. Likewise, marine organisms that are vulnerable to plastic ingestion or entanglement have uneven distributions. Understanding where wildlife encounters plastic is crucial for targeting research and mitigation. Oceanic seabirds, particularly petrels, frequently ingest plastic, are highly threatened, and cover vast distances during foraging and migration. However, the spatial overlap between petrels and plastics is poorly understood. Here we combine marine plastic density estimates with individual movement data for 7137 birds of 77 petrel species to estimate relative exposure risk. We identify high exposure risk areas in the Mediterranean and Black seas, and …

The Development Of The Cognitive Assessment For Tagalog Speakers (Cats): A Culturally And Linguistically Tailored Test Battery For Filipino Americans, Jessica De Leon, Ivan Bondoc, Eugenie Mamuyac, Lainie Posecion, Eduardo Europa, Lolita C. Kintanar, Niecholle Roco, Mikkael Lamoca, Danielle P. Escueta, Van M.Ta Park

Faculty Research, Scholarly, and Creative Activity

INTRODUCTION: Filipino Americans are one of the largest Asian American and Pacific Islander (AAPI) populations in the United States (US). Previous studies suggest that Filipino Americans have one of the highest incidence rates of Alzheimer's disease and related dementias (ADRD) among AAPI subgroups. Despite the expected increase in Filipino Americans with ADRD, no studies to-date have validated neuropsychological measures in the United States for speakers of Tagalog, a major language spoken by Filipino Americans. A significant barrier to dementia care and diagnosis is the lack of linguistically and socioculturally appropriate cognitive tasks for Tagalog speakers. To address this need, we …

Single-Cell Quantification Of Ribosome Occupancy In Early Mouse Development, Hakan Ozadam, Tori Tonn, Crystal M. Han, Alia Segura, Ian Hoskins, Shilpa Rao, Vighnesh Ghatpande, Duc Tran, David Catoe, Marc Salit, Can Cenik

Faculty Research, Scholarly, and Creative Activity

Translation regulation is critical for early mammalian embryonic development1. However, previous studies had been restricted to bulk measurements2, precluding precise determination of translation regulation including allele-specific analyses. Here, to address this challenge, we developed a novel microfluidic isotachophoresis (ITP) approach, named RIBOsome profiling via ITP (Ribo-ITP), and characterized translation in single oocytes and embryos during early mouse development. We identified differential translation efficiency as a key mechanism regulating genes involved in centrosome organization and N 6-methyladenosine modification of RNAs. Our high-coverage measurements enabled, to our knowledge, the first analysis of allele-specific ribosome engagement in early development. These led to the …

Sustainability Of Open-Access Author Fund: A Case Study Of Faculty Usage Patterns And Apc Cost, Jayati Chaudhuri, Adele Dobry

Library Philosophy and Practice (e-journal)

The California State University, Los Angeles Library established a pilot program on Open-Access (OA) Author Fund in 2018. This article presents information about the management of the University Library's Open-Access Author Fund. Particularly, this article focuses on faculty usage of the OA Author Fund by colleges, disciplines, and publishers. Additionally, the authors examined the article processing charges (APCs) and self-archiving policies of the top open-access journals where Cal State LA faculty publish. This analysis will assist the University Library’s Open-Access Group to understand if the University Library needs to provide additional funding and explore new ways to sustain the funding. …

Sleep Is Required To Consolidate Odor Memory And Remodel Olfactory Synapses, Rashmi Chandra, Fatima Farah, Fernando Muñoz-Lobato, Anirudh Bokka, Kelli L. Benedetti, Chantal Brueggemann, Mashel Fatema A. Saifuddin, Julia M. Miller, Joy Li, Eric Chang, Aruna Varshney, Vanessa Jimenez, Anjana Baradwaj, Cibelle Nassif, Sara Alladin, Kristine Andersen, Angel J. Garcia, Veronica Bi, Sarah K. Nordquist, Raymond L. Dunn, Vanessa Garcia, Kateryna Tokalenko, Emily Soohoo, Fabiola Briseno, Sukhdeep Kaur, Malcolm Harris, Hazel Guillen, Decklin Byrd, Brandon Fung, Andrew E. Bykov, Emma Odisho, Bryan Tsujimoto, Alan Tran, Alex Duong, Kevin C. Daigle, Rebekka Paisner, Carlos E. Zuazo, Christine Lin, Aarati Asundi, Matthew A. Churgin, Christopher Fang-Yen, Martina Bremer, Saul Kato, Miri Vanhoven, Noëlle D. L’Étoile

Faculty Research, Scholarly, and Creative Activity

Animals with complex nervous systems demand sleep for memory consolidation and synaptic remodeling. Here, we show that, although the Caenorhabditis elegans nervous system has a limited number of neurons, sleep is necessary for both processes. In addition, it is unclear if, in any system, sleep collaborates with experience to alter synapses between specific neurons and whether this ultimately affects behavior. C. elegans neurons have defined connections and well-described contributions to behavior. We show that spaced odor-training and post-training sleep induce long-term memory. Memory consolidation, but not acquisition, requires a pair of interneurons, the AIYs, which play a role in odor-seeking …

Modeling The Recent Changes Of Phytoplankton Blooms Dynamics In The Arctic Ocean, Manfredi Manizza, Dustin Carroll, Dimitris Menemenlis, Hong Zhang, Charles E. Miller

Faculty Research, Scholarly, and Creative Activity

In recent decades, Arctic Ocean (AO) sea ice has experienced intense climate-related modifications, which coincide with earlier melting in spring and delayed formation in the fall. In this study, we use the ECCO2-Darwin ocean biogeochemistry model to gain a mechanistic understanding of how changes in sea-ice seasonality impact the phenology of AO phytoplankton blooms. We use a 2006–2013 simulation to show that early sea-ice melt in 2007 and 2012 triggers earlier phytoplankton blooms (∼1 month earlier compared to other years) and that the blooms dissipate earlier due to more rapid nutrient depletion in the surface AO. Our simulation also confirms …

Smartlact8: A Bio-Inspired Robotic Breast Pump For Customized And Comfort Milk Expression, Yuying Li, Marlenne Valadez Lozano, David Peña, Ish Kumar Gulati, Lin Jiang

Faculty Research, Scholarly, and Creative Activity

According to the 2018 National Immunization Survey conducted by the Center for Disease Control and Prevention (CDC), 83.9% of the breastfeeding mothers in the United States have used a breast pump at least once. However, the majority of existing products use a vacuum-only mechanism to extract milk. This causes common breast injuries such as nipple soreness, breast-tissue damage, and lactation complications after pumping. The objective of this work was to develop a bio-inspired breast pump prototype, named as SmartLac8, that can mimic infant suckling patterns. The input vacuum pressure pattern and compression forces are inspired from term infants’ natural oral …

Reading On Paper: Exploring Trends Of Using Printed Books Among University Students And Shaping Reading Culture, Md. Nazim Department Of Information Science And Library Management, Md. Ekbal Hossain Department Of Information Science And Library Management, Shakibul Hsasn Department Of Theatre, Faisal Nomani Department Of Information Science And Library Management

Library Philosophy and Practice (e-journal)

Reading is a basic learning tool for an individual that begins early and continues throughout life. It would appear that the world is digitally at one's fingertips, with everything connected to the internet with the increasing dominance of digital reading over paper reading. On the other hand, it is fair to assume that books will continue to be printed for a very long time despite the rise in e-book readers. The aim of this paper is to demonstrate the actual trends in using printed books among university students. The study collected data from 233 undergraduate students from different Four (04) …

Curriculum Evaluation Of The Academy Of Global Logistics Program: Connections To Stem Education, Ann Y. Kim, Tyler Reeb, Jaylee Jordan, Youngjin Song

Mineta Transportation Institute

The Academy of Global Logistics (AGL) is a career technical education program developed in collaboration with the Port of Long Beach and the Long Beach Unified School District and with support from the Center for International Trade and Transportation. Students enrolled in the program, implemented at a high school in Long Beach, CA, learn global logistics and supply chain management over the course of their high school career. The program culminates in a capstone project that is evaluated by industry leaders. This research project applies qualitative coding methods to find connections between the AGL curriculum and CA mathematics and science …

Health Information Provision And Utilization As Predictors Of Safe Health Practices Of Nursing Mothers In Imo State, Nigeria, Pauline Iroeze, Ijeoma I. Bernard

Library Philosophy and Practice (e-journal)

Abstract

The study investigated health information provision and utilization as predictors of safe health practices of nursing mothers in Imo State. Four research purposes and four research questions guided the study. The researcher adopted descriptive design. The population of the study is 9735 nursing mothers in Okigwe Senatorial Zone, Imo State. Using proportionate sampling, 94 nursing mothers were selected from each of the six local government areas in Okigwe Zone, totaling 564. A researcher-made questionnaire “Information Provision and Utilization on Safe Health Practices of Nursing Mothers in Imo State Questionnaire” (IPAUOSHPONIMQ) was used as instrument for data collection. The …