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The Effects Of 712-Dimethylbenz (A) Anthracene On Ovarian Steroidogenesis And Uterine Growth In The Mouse, Jeffrey Lynn Clark

Biological Sciences Theses & Dissertations

It has been previously demonstrated that 7,12-dimethylbenz(a) anthracene (DMBA) is an initiator of ovarian granulosa cell tumors. It is also known that DMBA leads to destruction of primordial oocytes, and that tumor induction may involve the interaction of pituitary gonadotropins. However, the effects of DMBA on ovarian steroidogenesis and uterine growth in relation to the tumorigenic process have not been investigated. Therefore, the present study was conducted to examine these parameters using adult C57Bl/6N mice treated in proestrus and diestrus II with 0.1 ml corn oil with or without 1.5 mg DMBA and killed 44 hours later. The uteri and …

The Effects Of Salinity On The Growth And Survival Of The Crayfish, Procambarus Actus Actus (Girard), Emily Deaver

OES Theses and Dissertations

The white river crayfish, Procambarus acutus acutus was tested in low salinity water for growth and survival. Thirty animals were placed in each test pool at O, 4 and 8 ppt and grown for 75 days. Growth was defined as increase in carapace length and wet weight. Crayfish grew at a faster rate and had the lowest mortality at 4 ppt. Water hardness, defined as the total concentration of calcium and magnesium expressed as the calcium carbonate equivalent, ranged from 35 mg/1 to 195 mg/1. Pools of crayfish tested at concentrations of approximately 100 mg/1 total hardness exhibited the best …

Multiple Forms Of Nicotinamide Adenine Dinucleotide Glycohydrolase From Bull Semen, Stanley D. Yeatts Ii

Chemistry & Biochemistry Theses & Dissertations

Nicotinamide adenine dinucleotide glycohydrolase (NADase) of bull semen is an extracellular, soluble enzyme capable of catalyzing the hydrolysis of the nicotinamide N-ribosidic linkage of NAD⁺. In search for a rapid, simple procedure for the preparation of this enzyme, it was found that Matrex Gel Red A (Amicon) column packing served very well as an affinity gel for NADase. The enzyme was applied to the Matrex Red A column (21 cm x 1.5 cm) in 10 mM sodium phosphate buffer, pH 6.0. Surprisingly, two fractions of NADase activity were obtained when the column was eluted with a sodium chloride …

The Effect Of The Herbicide Metolachlor On Nutrient Levels In Zea Mays L., Tamsey Dawn Warren Ellis

Biological Sciences Theses & Dissertations

An investigation of the effect of metolachlor, a chloracetamide herbicide released for agricultural purpose during the Spring of 1977, on plant tissue levels of phosphorus, potassium, calcium and magnesium in juvenile sweet corn and the recovery rate of sweet corn exposed to metolachlor from any plant nutrient effects when the herbicide was removed.

An increase of metolachlor concentration caused a progressive reduction in growth (height and dry weight) of the leafy portion of juvenile sweet corn. At metolachlor concentrations of 0.16 ppm and higher pronounced stunting and twisting were observed in the plants.

There was a progressive reduction in the …

Germination And Haustorial Development In Orobanche Minor Smith (Orobanchaceae), David L. Spelce

Biological Sciences Theses & Dissertations

Orobanche minor Smith is an introduced root parasite that is known to attack some commercial crops. Germination occurs only after a preconditioning period involving moisture which must be followed by a chemical stimulant produced by the host root. Strigol and strigol analogs (GR-compounds} have been reported to germinate some species of Orobanche. Statistical tests indicate a significant difference between the effectiveness of strigol, GR-7 and GR-45 (P = 0.001).

The embryo is an undifferentiated mass of cells. During germination, the radicular pole of the embryo differentiates into the radicle, while the plumular pole remains undifferentiated. The radicle forms the primary …

The Effects Of Elevated Calcium Chloride And Magnesium Chloride Levels On Larval Growth And Survival Of Macrobrachium Rosenbergii (De Man) (Decapoda Palaemonidae) Reared In The Laboratory, James Thomas Michaels Ii

OES Theses and Dissertations

The effects of elevated CaCl₂ , MgCl₂ , combination CaCl₂ and MgCl₂ concentrations and their associated ionic strengths on larval growth and survival of the freshwater prawn, Macrobrachium rosenbergii were investigated in the laboratory. Larvae were reared in diluted seawater of 15 parts per thousand salinity with elevated calcium concentrations of 330 parts per million and elevated magnesium concentrations of 1275 parts per million. Control larvae were also reared with elevated NaCl concentrations to duplicate the ionic strength increases of the combination CaCl₂ - MgCl₂ test solution which had the highest ionic strength. A …

Filtration Ingestion And Assimilation Rates Of The Mysid Shrimp Neomysis Americana Smith, Fed Three Food Sources, Robert W. Grabb

OES Theses and Dissertations

Laboratory grazing and assimilation experiments were conducted on the mysid shrimp Neomysis americana in an attempt to assess the suitability of three potential food sources. It was hypothesized that the smaller size classes were primarily herbivores, not becoming omnivorous until attaining lengths of approximately 5-6 mm. Four size classes of mysids from the summer generation, juveniles, immature, adult males, and adult ovigerous females were each fed three concentrations of Artemia salina nauplii, the rotifer Brachionus plicatilis, and the diatom Coscinodiscus lineatus. The mean lengths of the size classes utilized, plus or minus one standard deviation, were 2.5 …

Distribution Of The Dinoflagellate Symbiosis In Astragia Danae As Affected By Light Attenuation, Michael Perry Weston

OES Theses and Dissertations

Spatial zonation of symbiotic and aposymbiotic forms of the temperate coral Astrangia danae provides evidence that light attenuation is an important factor in the distribution of this animal. At four locations in the lower Chesapeake Bay and adjacent offshore waters, symbiotic colonies were observed only in well illuminated surface waters, while aposymbiotic colonies were observed in less illuminated deeper waters. A transition zone exists in which both colony types are found. Colonies on substrates forming a plane parallel to the sea-surface receive a greater radiant flux than colonies on vertical substrates. This extends the depth range of symbiotic colonies located …

Sulfatides As Opiate Receptors: An Evaluation Of The Opiate Binding Potential Of Sulfogalactosylgylcerolipid, Darby Geoghegan Hand

Chemistry & Biochemistry Theses & Dissertations

Cerebroside sulfate (CS) has recently been shown to bind opiate drugs in a pharmacologically relevent manner and therefore satisfy the structural requirements of an opiate receptor. Seminolipid (SGG), a novel sulfated galactosyl. glycerolipid that has been isolated from the testes of a variety of mammals, may be viewed as a structural analog of CS. In view of this structural similarity, it is reasonable to presume that SGG may also bind opiate drugs. The isolation of SGG from boar testes was accomplished using silica gel dry column chromotography with a chloroform: methanol water solvent system. The structure and purity of the …

Attitudes Of Students In Selected Virginia Colleges And Universities Related To Death And Dying, Jacqueline J. Rochele

Human Movement Studies & Special Education Theses & Dissertations

The purpose of this study was to determine the attitudes toward death and dying of college students in selected colleges and universi­ties in Virginia. The purpose was to identify attitudinal differences relating to the characteristics of sex, race, age, religious preference, degree of religiosity, socio-economic area, income level, marital status, political viewpoints, imminent personal death, and personal death experience.

The Complete Inventory Price Death Attitude Scale was administered to 886 college students during the 1979-80 academic school year. Data were statistically analyzed by the Discriminant Analysis Technique and the Cattell Coefficient of Profile Similarity.

Data were analyzed in terms of …

The Association Of Semen Factors With The Recovery Of Ureaplasma Urealyticum, Rebecca D. Cintron

Biological Sciences Theses & Dissertations

Fifty-six semen samples from males under examination for suspected subfertility were examined to determine if alterations in specific semen parameters could be associated with the presence or absence or Ureaplasma urealvticum. Twenty-seven (48.2%) of the specimens cultured positive for U· urealyticum in U9B urease broth and A-7 differential agar. Both positive and negative specimens have a normal average motility rating. Overall sperm counts (millions per ml) were slightly lower and ejaculate volumes higher in U. urealvticum positive samples. Morphological abnormalicies were not found more frequently in U. urealyticum positive samples. No statistically significant difference between seminal parameters of U. …

Macrobenthic Communities Of An Industrialized Seaport Ecosystem: The Southern Branch Of The Elizabeth River, Virginia, Steven D. Hawthorne

Biological Sciences Theses & Dissertations

Macrobenthic invertebrates of an industrialized seaport ecosystem were studied seasonally from October, 1977 through July, 1978. Five stations were selected along the Southern Branch of the Elizabeth River, Virginia. The stations ranged from an area of heavy industrialization to a non-urbanized environment. At each station, bottom water was measured for salinity, temperature, dissolved oxygen, and pH. Within each sampling area, macrobenthic infauna! invertebrates were collected from in and out of channel sites along with sediment samples for various types of physicochemical analysis.

Community structure was spatially homogeneous at station sites and throughout the stations along the River. Slightly acidic pH …

The Feasibility Of Commercial Scale Culture Of Atlantic Sturgeon, Acipenser Oxyrhynchus, Christopher John Coffing

Biological Sciences Theses & Dissertations

Abstract unavailable.

Structural Studies Of Preimplantation Rabbit Embryos Using Ftir Atr, Amy R. Moler-Booth

Chemistry & Biochemistry Theses & Dissertations

FTIR was used in conjunction with a surface selective technique, known as attenuated total reflectance (ATR), to study the infrared spectra of preimplantation rabbit embryos. The rabbit embryos ranged in aged from 3 to 6 days. Since an embryo is mostly water, deuteration of the embryo was necessary to eliminate the intense water background present in the mid-infrared region. A set of spectral standards were tested, in order to identify the bands seen in embryo spectra. By analyzing and comparing the spectra from 3, 4, 5 and 6 day embryos, differences could be noted. In particular, bands defined as Amide …

Isolation And Enumeration Of Enteric Viruses From Primary Wastewater Sludge, Michael Wayne Nath

Biological Sciences Theses & Dissertations

The presence of large numbers of enteric viruses in wastewaters has resulted in concern regarding their significance and consequences for humans. Assessments of relative significance or consequences may most accurately be made following precise measurements of the range of virus types and amounts in wastewaters and sludges and after epidemiological considerations. In this study, results are presented pertaining to enteric virus isolation, concentration, and enumeration from primary wastewater sludges.

As an integral part of a comparison of isolation procedures, several anionic surfactants were examined for extraction of enteric viruses from primary wastewater sludges. Plaquing efficiencies for poliovirus type 2 and …

Old Dominion University: A Half Century Of Service, James R. Sweeney

History Faculty Bookshelf

Dr. James R. Sweeney has written an informative account of the university's first half-century. It is a history of growth from a small two-year branch of the College of William and Mary to a state-supported university that has gained its own national reputation.

[From the "Introduction," by Alfred B. Rollins Jr, Aug. 14, 1980]

The Association Of 5.8 S With 28s Ribosomal Rna, Nandita Banerjee

Chemistry & Biochemistry Theses & Dissertations

5.8S rRNA is a low molecular weight ribosomal RNA which is noncovalently bonded to the larger ribosomal subunit 28S rRNA; through its 3' end and through its 5' end. This interaction is an integral part of the ribosome, and plays an important role in the ribosome structure and function.

There is a high degree of homology between the 5.8S rRNA primary structures of rat, turtle and chicken. The base sequence of rat 5.8S rRNA differs only in one position from that of turtle and in three positions fr.om that of cl1.icken. Tl1ere is a single purine substitution at the 5' …

Aspects Of Larval Ecology Of Squilla Empusa (Crustacea, Stomatopoda) In Chesapeake Bay, Steven G. Morgan

OES Faculty Publications

Larvae of Squilla empusa were collected from the plankton and were laboratory-reared in 16 combinations of temperature and salinity to determine their tolerances. Larvae survived longer and molted more frequently when reared at 25%, and 20° or 25° C, which corresponds to the natural conditions of Chesapeake Bay when the larvae were collected.

A 2 year planktonic survey conducted in the lower region of the bay by the Virginia Institute of Marine Sciences was compared with a survey made at the bay mouth in 1976. The seasonal occurrence of Squilla empusa larvae extended from the last week of July until …

The Determination Of The Compatibility Between The Reasons For Attending And The Career Goals Of Secondary Students At The Norfolk Technical-Vocational Center, Judith A. Wiltshire

OTS Master's Level Projects & Papers

The purpose of this study was to answer the following questions: 1. Why do students elect the programs at NTVC? 2. What do these students intend to do after high school graduation?

A Study To Determine The Awareness Of Tidewater Industrial Arts Teachers Of The Procedures For Proper Handling Of Toxic Substances, Brigitte Graudins Valesey

OTS Master's Level Projects & Papers

This study sought to answer the following questions: 1. What are the toxic substances used most frequently in industrial arts laboratories? 2. How are each of these substances stored and handled? 3. Who most frequently handles these substances? 4. Are both students and instructors adequately informed of the potential hazards relating to the use of these substances? 5. What recommendations should be made to increase teacher awareness of the harmful side effects of toxic substances, both on them and their students?

A Study To Determine The Acceptance By Potential Employers Of A Cooperative Education Program For Gifted And Talented Students In Newport News Public Schools, Deborah Smith Buchanan

OTS Master's Level Projects & Papers

In order to plan for this proposed cooperative program, it was necessary to gain specific information from the business community on the Peninsula. The goals of this study were: 1. To determine the job placements for gifted and talented students that would be available on the peninsula; 2. To determine the variety of occupational areas that would provide assistance to the program; 3. To determine the job-entry skills that prospective employers would require of a student in the program.

Ocean Chlorophyll Studies From A U-2 Aircraft Platform, Hongsuk H. Kim, Charles R. Mcclain, Lamdin R. Blaine, William D. Hart, Larry P. Atkinson, James A. Yoder

CCPO Publications

Chlorophyll gradient maps of large ocean areas were generated from U-2/OCS data obtained over test sites in the Pacific and the Atlantic Oceans. The delineation of oceanic features using the upward radiant intensity relies on an analysis method which presupposes that radiation backscattered from the atmosphere and the ocean surface can be properly modeled by using a measurement made at 778 nm. The calculation of atmospheric radiance was performed by using a method developed by J.V. Dave. An estimation of the chlorophyll concentration is performed by properly ratioing radiances measured at 472 and 548 nm after removing the atmospheric effects. …

Phytoplankton Studies Within The Virginia Barrier Islands I. Seasonal Study Of Phytoplankton In Goose Lake, Parramore Island, Harold G. Marshall

Biological Sciences Faculty Publications

The phytoplankton of Goose Lake, an oligohaline lake on Parramore Island, was studied for one year. The populations consisted primarily of ultraplankton and nanoplankton-sized forms with diatoms and chlorophyceans dominant most of the year. A general pattern of seasonally higher cell concentrations in early summer and fall was noted, with an unidentified ultraplankton-sized component prominent throughout the collection period. A list of 154 species is given.

Morphological Variation Among 14 Species Of Callogobius Bleeker (Pisces: Gobiidae), James Francis Mckinney

Biological Sciences Theses & Dissertations

A survey of the variation among the species of the Callogobius complex has been accomplished in order to determine if recognition of more than one genus is warranted for this assemblage of species. Emphasis has been placed on the osteological variation. Relationships among certain species within the Callogobius complex have been exposed. Although there are two well defined species groups within the complex, it is felt that inclusion of all of the examined species within the single genus Callogobius is the most parsimonious course of action.

Multiple Methods Of Analysis Of 2'-O-Methylation In 5.8s Rrna, Sharon H. Ryan

Chemistry & Biochemistry Theses & Dissertations

5.8S rRNA is a low molecular weight ribosomal RNA which is found hydrogen bonded to 28S rRNA in the eucaryotic cell. There are two nucleotides which have 2'-0-methylations; a guanine residue at position 77 is fully methylated and a uridine residue at position 14 which is partially methylated. This partial 2'-0-methylation of the uridine residue has been found to vary with the tissue source, with the highest level in normal tissue and the lowest level in neoplastic tissue. In order to study the significance of this site-specific methylation, a reliable and convenient method of analysis was needed.

Early studies on …

The Synthesis And Evaluation Of The Potential Antineoplastic Activity Of The Semicarbazone And The Thiosemicarbazone Derivatives Of The Di-2-Pyridyl Ketone And The 1,8-Diazafluorenone Ring Systems, Adriene Y. Principe

Chemistry & Biochemistry Theses & Dissertations

A number of' a-(N)-heterocyclic thiosemicarbazones have been synthesized and evaluated for potential antineoplastic activity since Brockman and colleagues2 reported the antineoplastic activity of' 2-f'ormylpyridine thiosemicarbazone (1). The primary objective of' this research project has been the synthesis and characterization of' the semicarbazone and the thiosemicarbazone derivatives of' the di-2-pyridyl ketone, (9-a) and (9-b), and the 1,8-diazaf'luorenone, (10-a) and (10-b), ring systems, as well as the evaluation of' these compounds for both in vitro and in vivo antineoplastic activity against Ehrlich ascites tumor cells in mice. These studies have suggested that di-2-pyridyl ketone thiosemicarbazone (9-b) is as effective as 2-formylpyridine …

Structure Of A Subtidal Benthic Community Near Cape Charles, Virginia, R. Michael Ewing

Biological Sciences Theses & Dissertations

Species composition, distribution, and abundance of benthic invertebrates of a subtidal community were examined at a location on the lower Delmarva Peninsula near Cape Charles, Virginia. Macrobenthos was sampled quarterly between June, 1977 and June, 1978 at nine sites along a transect in Old Plantation Creek and the adjacent offshore area. Spatial and temporal patterns of numerically important species are discussed.

Sampling sites and species were grouped by a numerical classification strategy which consisted of the Bray-Curtis similarity measure and group-average clustering. Three faunal assemblages were identified with the aid of constancy and fidelity nodal analyses: (1) a tidal creek …

The Development Of Techniques For The Enumeration Of Selected Pathogenic Bacteria In Lynnhaven Estuarine Sediments, Mark T. Frank

Biological Sciences Theses & Dissertations

Methodologies for the improved recovery of selected pathogenic bacteria from estuarine sediments were investigated. Laboratory and field studies were conducted to document the sensitivity of the techniques for isolating Salmonella species, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. Vibrio parahaemolyticus-like organisms were enriched in a seawater yeast extract broth and isolated from Thiosulfate Citrate Bile Salts Sucrose plated media. Enrichment procedures with and without elevated temperatures and subsequent transfers to selective and differential plated media have been examined for the isolation of Salmonella species and P. aeruginosa. These pathogens were initially assayed for utilizing primary enrichments of asparagine …

Feeding Morphologies And Distribution Patterns Of Marine Cladocera In The Lower Chesapeake Bay, Sandra Layne Gilchrist

OES Theses and Dissertations

Temporal and spatial distributions of marine cladocera in the lower Chesapeake Bay estuary were observed over a year-long sampling period. The distributions of cladoceran species throughout the Bay were studied in relation to temperature and salinity. A survey of the species over the entire Bay is included to establish distribution patterns of the various species during a short time period.

Theoretical mathematical principles were used to predict feeding abilities of the Bay species. Mouthparts are described qualitatively and quantitatively to infer function. A mandibular index developed by Itoh (1970) was revised and employed in evaluating feeding methods interspecifically and interspecifically. …

The Spatial And Temporal Distribution Of Fish Eggs And Larvae In Hempstead Bay Estuary, Joseph Adelard Lessard

OES Theses and Dissertations

The ichthyoplankton of Hempstead Bay estuary was sampled from December 1972 to mid March 1974. Eleven stations were sampled by towing a 0.5 meter diameter plankton net. Eggs were most abundant during June and July with an average concentration calculated to be above 5,500/100m³. Egg densities were the lowest during the fall and winter. Larvae were most frequently taken from March through July with densities varying greatly among the samples. The eggs of Brevoortia tyrannus, Anchoa mitchilli, Tautoga onitis, Tautogolabrus adspersus, and Scophthalmus aguosus and the larvae of Menidia menidia, Ammodvtes hexapterus and Pseudopleuronectes americanus constituted most …