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A Multivariate Characterization Of Assemblages Of Planktonic Mysids Decapods And Sergestids In The Chesapeake Bay Mouth Area, John Charles Seibel

Biological Sciences Theses & Dissertations

This study is the first to focus on the assemblages of the planktonic mysids, decapods, and sergestids found in the lower Chesapeake Bay. The assemblages of these organisms in the lower Chesapeake Bay and nearby shelf were characterized using three different statistical approaches, and the methods used were evaluated for their effectiveness at delineating ecologically meaningful assemblages. Three stations were sampled across the Chesapeake mouth and one offshore, with oblique and neuston net tows. Twenty-seven larval stages, representing at least 23 species, were found in sufficient numbers to be analyzed statistically. The statistical approaches were each based upon a different …

Evidence For The Sensory Receptor(S) Of The Mounting Sex Pheromone In Two Species Of Ixodid Ticks, Dermacentor Variabilis (Say) And Dermacentor Andersoni Stiles, James Stephen Phillips

Biological Sciences Theses & Dissertations

The foreleg claw sensilla were determined to be the receptors of the female contact sex pheromone, MSP (mounting sex pheromone), in D. variabilis, D. andersoni and A. americanum male ticks. In all three tick species, the claw sensilla consists of six anteriorly-directed setae arranged in three symmetrical pairs, two each on the opposite sides of the apotele of the claw and one on the ventral side. Behavioral bioassays and morphological study of these setae revealed that only the dorsal and middle (= lateral) pairs of claw sensilla are mechanogustatory, while the ventral pair are strictly mechanoreceptors. The dorsal and …

Retention Of Cellular Viability And Growth Potential In Cryopreserved Bovine Arterial Tissue: Implications For Clinical Use Of Cryopreserved Vascular Allografts, Kip G. Gardner

Biological Sciences Theses & Dissertations

Segments of bovine calf aortas and pulmonary arteries were cryopreserved at -196° C for two weeks to 18 months and subsequently thawed. Cellular morphology, viability, and growth potential in these segments were then compared with the same attributes in segments from fresh (control) arteries. Scanning electron microscopy revealed no disruption of intimal surfaces attributable to cryopreservation. Endothelial cells were successfully cultured from all cryopreserved arterial segments. Endothelial monolayers grown from cryopreserved arteries had a cobblestone appearance and expressed Factor VIII antigen but not smooth muscle alpha-actin. Plating efficiency in primary cultures of endothelial cells was lower for cryopreserved arteries than …

Structural Characterization Of Fucoidan And Its Role In The Hemizona Assay, Manish S. Patankar

Chemistry & Biochemistry Theses & Dissertations

Fucoidan is a polysaccharide commonly found in brown algae. It is a sulfated polymer of the monosaccharide L-fucose. Fucoidan is thought to function in water retention by algae. Commercially available fucoidan is extracted from a brown algae called Eurus vesiculosus.

This polysaccharide has immense potential as a biologically active agent: (i) it exhibits anticoagulant action, (ii) it has also been reported to block retroviral replication and (iii) it is capable of blocking sperm-egg binding in sea urchin, mouse and rabbit. Recently, fucoidan has also been shown to block the interaction of human sperm and egg in the hemizona assay …

Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr.

Bioelectrics Publications

The temperature-sensitive Saccharomyces cerevisiae mutant sec59 accumulates inactive and incompletely glycosylated protein precursors in its endoplasmic reticulum at the restrictive temperature. O-mannosylation and glycosyl phosphatidylinositol membrane anchoring of protein are also abolished, consistent with a deficiency in dolichyl phosphate mannose. Membranes prepared from sec59 cells that had been shifted to the restrictive temperature, however, made normal amounts of dolichyl phosphate mannose when exogenous dolichyl phosphate was supplied, but dolichyl phosphate mannose synthesis was severely depressed in the absence of exogenous dolichyl phosphate. Quantitative measurements of dolichyl phosphate in sec59 cells showed that the levels were decreased to 48% of wild …

Increasing International Military Interdependence: Defense Cooperation In The New World Order, Sheila Callaham-Gay

Graduate Program in International Studies Theses & Dissertations

This thesis examines whether military interdependence among states is increasing or decreasing. Although it is impossible to predict the future, it can be deduced that military interaction is increasing as a result of current world events and stated Presidential policy objectives. In order for interdependency to reach fruition governments must create policy which allows mutual goals to be realized. If military-to-military programs contribute to U.S. political objectives then government policy toward military interdependence should allow the U.S. military to act as a catalyst for international cooperation as well as the guardian of U.S. security interests. Whether global peace and security …

An Application Of Neural Networks In Data Communication Real-Time Resource Reallocation, Qing Fan

Electrical & Computer Engineering Theses & Dissertations

This thesis presents an application of artificial neural networks in real-time resource reallocation, a methodology used in the implementation of an intelligent interface node in the Computer Integrated Manufacturing (CIM) environment. In particular, the problem is formulated using a Hopfield neural network model. The real-time reallocation problem is mapped into a two-dimensional matrix of neurons similar to Hopfield and Tank's approach to the traveling salesman problem (TSP) . An energy function is formulated in terms of the hard constraints and the solution cost. The interconnection weights and the input biases are determined by the energy function. It is shown through …

The Effects Of Stroke On The Comprehension Of Affective And Nonaffective Prosody, Mona Matheney Tiernan

Psychology Theses & Dissertations

The purpose of the present study was to examine the effects of stroke on the comprehension of prosody. Thirty two inpatient volunteers (16 with right hemisphere and 16 with left hemisphere stroke) and 16 controls participated in the study. A 3(Group) x 2(Gender) x 2(Task) mixed design was used. The independent variables were the subject group, gender and the prosody task (affective or nonaffective). The dependent variables were the percentage of correct responses to the affective and nonaffective prosody tasks. A 3(Group) x 2(Gender) x 2(Task) AN0VA revealed a significant main effect for group, E

Biochemical Investigation Of Gout And Its Familial Incidences, Chin-Ling Hsu

Chemistry & Biochemistry Theses & Dissertations

Gout is a chronic metabolic disorder caused by deposition of uric acid on the joint. It is categorized into two types: primary and secondary. Primary gout is uric acid overproduction, caused by excessive synthesis of the purine precursors. Secondary gout occurs also as the result of overproduction or decreased renal excretion of uric acid resulting from other disease processes or medication.

The two purine bases, hypoxanthine and xanthine, liberated from ribosides by the action of nucleoside phosphorylase, are degraded to uric acid as the final excretory product in the purine degradation pathway. Hypoxanthine and xanthine are the physiological substrates of …

Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard

Chemistry & Biochemistry Theses & Dissertations

The human immunodeficiency virus (HIV) reverse transcriptase (RT) (EC 2.7.7.49) is the central replication enzyme for HIV. In general, the kinetic mechanism for this and all other polymerases involves the ordered binding of two substrates: a primer-template (PT) followed by a deoxyribonucleoside triphosphate (dNTP). Previous investigations prompted this research when it was discovered that the substrate dNTP, in absence of PT, could protect a recombinant c-terminal mutant HIV-1 RT from inhibition by pyridoxal-5'-monophosphate (PLP), an active-site dNTP inhibitor. In contrast, the non-mutant recombinant HIV-1 RT required both substrates for protection from PLP inhibition. This investigation sought to determine if this …

Mat Movement In Coastal Louisiana Marshes: Effect Of Salinity And Inundation On Vegetation And Nutrient Levels, Christopher Martin Swarzenski

Biological Sciences Theses & Dissertations

The present research compared and contrasted the physical structure of floating and rooted marshes, their differing responses to open-water salinities and inundation, as well as the nutrient distribution in the porewaters and sediment. The effects of the physical differences in the two marsh types on the ocurrence of the dominant emergent vegetation was discussed.

The main difference in physical structure of the two marsh types was the presence of a mineral, non-buoyant layer at 25-45 cm depth in the rooted marsh, which could serve as an anchor for the overlying highly organic mat layer found in both marsh types. Porewater …

The Functional Role Of Peripheral Nerves In Ige-Mediated Inflammation, Gary Wright Miller

Biological Sciences Theses & Dissertations

IgE-mediated inflammation was measured in mouse footpads with and without sciatic innervation. Mice were passively sensitized with IgE anti-dinitrophenol, a monoclonal antibody. Antigen-induced swelling was reduced by 26% by sciatic nerve transection. Whether transection occurred 10 days prior or immediately prior to antigen challenge did not affect the swelling response. The full inflammatory response was restored by antidromic stimulation of the distal portion of the cut nerve, but only in the freshly cut nerve. Antigen-induced swelling was unaffected by rhizotomy or spinal nerve transection. Pre-treatment with capsaicin reduced the swelling to a level similar to sciatic nerve transection. Treatment with …

Seasonal Changes In Brown Adipose Tissue And Pelage Of The Southern Short-Tailed Shrew Blarina Carolinensis, Elisa Michelle Dew

Biological Sciences Theses & Dissertations

Winter survival mechanisms in the southern short-tailed shrew, Blarina carolinensis, were studied by examining cellular changes in interscapular brown adipose tissue (ISBAT) and pelage characteristics in specimens collected each month of the year in eastern Virginia. On capture, each specimen was anesthetized with ether and then perfused with a 2.5% glutaraldehyde-2% formaldehyde solution. ISBAT tissues were examined histologically with the transmission electron microscopy. Because BAT is know to produce heat by nonshivering thermogenesis and is more developed in winter animals than summer animals, it was hypothesized that mitochondrial area of BAT would be relatively greater in winter than in summer. …

Dynamics And Control Of A Five Degree-Of-Freedom Magnetic Suspension System, Anwar Mohammed Haj

Mechanical & Aerospace Engineering Theses & Dissertations

A large-gap magnetic suspension system with five degrees-of-freedom is presented. The system is multi-input multi-output with coupling between degrees-of-freedom. Simulation was performed on this multi degree-of-freedom system in order to control each degree-of-freedom separately. Two types of controllers are considered by adding white noise to a single degree-of-freedom system in order to test their behavior and determine which is the best choice for the system. The responses of the system are produced in continuous and discrete time where a sample interval and delay time was introduced. Using these responses, a comparison between each degree-of-freedom was made and the maximum value …

Differential Utilization Of Allochthonous And Autochthonous Carbon By Aquatic Insects Of Two Shrub-Steppe Desert Spring-Streams: A Stable Carbon Isotope Analysis And Critique Of The Method, Alfred Lynn Mize

Biological Sciences Theses & Dissertations

Stable carbon isotope analysis, a technique that measures the ratio of stable carbon-12 to stable carbon-13 in abiotic and biotic components of ecosystems, was used to assess the differential utilization of autochthonous and allochthonous carbon by aquatic insects of two cold desert spring-streams in the Columbia Basin of Washington State. The aquatic insects of Snively Springs, a heavily overgrown and densely shaded stream, has isotopic values close to that of stream aufwuchs (substrata scrubate) indicating substantial autochthonous carbon dependence. Aquatic insects of Douglas Creek, the more open and less shaded of the two streams, were equally dependent upon autochthonous and …

Making Salt, Lynn Dean Hunter

English Theses & Dissertations

Every day, when reading the paper or watching the six o'clock news, women and men living in the post-World War II era must confront existential issues of powerlessness, meaninglessness, freedom, and death.

Nevertheless, we persist in seeking order, beauty, justice, and eternity.

The seven stories in this collection portray characters caught in conflict between their desires for an ideal world and the poignantly tangible absence thereof.

Penguins Can't Fly And Women Don't Count: Language And Thought, Janet M. Bing

English Faculty Publications

Many people object to sexist and racist language partly because they assume that language not only reflects, but somehow affects attitudes. A one-to-one relationship between language and thought seems obvious to those who never question it, but the issue of whether language influences thought and behavior has been a matter of debate in philosophy even before Berkeley and Wittgenstein. Literary critics, particularly those who call themselves deconstructionists, are still debating to what extent language constructs reality.

The Relationship Of Educational Attainment And Socioeconomic Factors In The Health Behaviors Of An Urban Population, George C. Maihafer

Theses and Dissertations in Urban Services - Urban Education

The purpose of this study was two fold: 1) to investigate the effects that level of educational attainment and income have on the health behaviors of an urban population, and 2) to examine the effects the level of motivation and selected demographic characteristics (e.g., age, race, gender) have on the study sample's perceived satisfaction with the medical encounter. Based on the findings of the study, a model community health education program was proposed. The study sample was comprised of 150 volunteers seeking health care for chronic conditions at a family practice clinic. The Krantz Health Opinion Survey (HOS) measured the …

The American Schoolyard: A Critical Study Of Charles Bukowski's Short Fiction, Jonathan D. Weiss

English Theses & Dissertations

Charles Bukowski is one of America's most prolific writers. He has published forty-five books of poetry and prose and is translated in over a dozen languages, Recently he wrote the screenplay for the movie Barfly. However, despite his accomplishments, Bukowski has received little critical attention from the academic world. It is thought that his works are too violent or too pornographic to be deserving of critical attention. In this thesis, I attempt to show that this is not the case. Using Marxist, feminist, and Freudian analyses of Bukowski's short fiction, I show that Bukowski's work is, without a doubt, worthy …

Characterization Of A Novel Prostate Tumor-Associated Antigen, Grayson B. Lipford

Theses and Dissertations in Biomedical Sciences

The murine monoclonal antibodies (MAb) TURP-27 and HNK-1 have been shown to detect antigens that are heavily expressed by benign prostatic hyperplasia (BPH) and carcinoma of the prostate (CaP). Western blot analysis of prostate extracts, showed that MAb TURP-27 and MAb HNK-1 bound glycoproteins of 180, 140, 120, 100, 90 and 69 kDa. Studies have shown that the HNK-1 carbohydrate epitope may be involved in cell adhesion and that it is a component of several characterized adhesion proteins. TURP-27 was found to bind at least three of these adhesion proteins: neural cell adhesion molecules (N-CAM), myelin-associated glycoprotein (MAG) and a …

Factors Affecting The Kinetics Of Light Intensity Adaptation In Marine Phytoplankton, Chunzhi Guo

OES Theses and Dissertations

It has been suggested that the recent light history of phytoplankton and the kinetics of photoadaptation can be used to provide information about the vertical mixing processes in the upper mixed layer. To be useful as a parameter in a model of photoadaptation and vertical mixing, the response of a photoadaptive variable to changes in growth irradiance must be monotonic, significant, and comparable in time rate scale to mixing processes. Previous studies of photoadaptation kinetics have focused on the response of phytoplankton to changes in light intensity under continuous illumination. This dissertation attempts to elucidate the effects of light:dark cycle, …

The Growth And Bioenergetics Of Callinectes Sapidus Larvae And The Effects Of Diet Quality On Larval Physiology, James J. Pletl

OES Theses and Dissertations

Food quality, especially ω3 polyunsaturated fatty acids (PUFA), can affect the physiology and metabolic efficiency of larvae of the blue crab, Callinectes sapidus. These affects may correlate with success in development and juvenile recruitment.

Larvae were exposed to two diets differing quantitatively in ω3 PUFA and respiration, excretion, growth, and feeding rates were measured for each larval stage and the megalopal stage. The energy partitioned to molts and specific dynamic action was also measured. T

he ω3 PUFA enriched diet resulted in a lower respiration rate when compared to the unsupplemented diet, but there was little differences in excretion …

Optimization Of Cryopreservation Protocols For Cardiovascular Tissue, Jianfei Hu

Theses and Dissertations in Biomedical Sciences

The purpose of this research was to determine the effects of cryopreservation on the physical, biochemical and cellular aspects of heart valve tissue. The present study demonstrated that radiolabeled proline accumulation provides a quantitative estimate of total cellular metabolic viability. Radiolabeled inulin was demonstrated to be a useful marker for extracellular tissue space. Assessment of metabolic viability of the cellular population of leaflet tissue as a function of procurement and processing revealed that proline accumulation declined with increasing warm and cold ischemic times. Amphotericin B and streptomycin were toxic to the cells. Cefoxitin, lincomycin, polymyxin B, vancomycin and penicillin had …

Biochemical Analysis Of Human Seminal Plasma, Jamshed Ayub

Chemistry & Biochemistry Theses & Dissertations

Presently there is no single biochemical criterion for differentiating between a potentially fertile male and an infertile male. However, for evaluating male fertility the biochemical analysis of seminal plasma is a very convenient and quantitative technique if it is combined with careful clinical examination.

In seminal plasma analysis, fructose and proteins are chemical indicators of seminal vesicle activity, citric acid and acid phosphatase indicate prostate function while glycerolphosphorylcholine indicates epididymis activity. In this research these five indicators are studied and an attempt is made to establish their normal ranges.

This study shows that the data obtained from the seminal plasma …

The Relationship Between Gender And Empathy On A Telephone Hotline, George Shepeard

Psychology Theses & Dissertations

The impact of gender upon levels of empathy within the context of a volunteer telephone hotline was examined. Male and female confederates placed fictitious telephone calls to the hotline which were recorded and evaluated for state empathy. Trait empathy of the hotline volunteers was also assessed and results revealed no gender differences. A gender of caller by gender of telephone worker analysis of variance on state empathy ratings revealed no significant main effects or interactions. A significant negative correlation between age of the telephone worker and the empathy levels was found. The lack of expected findings is discussed in terms …

Wising Up The Marks: The Later Works Of William S. Burroughs, Stephen L. Starck

English Theses & Dissertations

The purpose of this paper is to investigate the later works of Williams. Burroughs, focusing on them as they articulate his theories of the individual. Following a brief look at each work (The Wild Boys, Exterminator!, Port of Saints, Cities of the Red Night, The Place of Dead Roads, The Western Lands) is a discussion of Burroughs' concerns about the individual, as expressed in his theories of government, religion, the body, and language. Burroughs instructs individuals, attempting to educate them to all the restraints imposed by society. The overall narrative structure of these six works is also discussed and then …

Biodegradation Of Crude Oil By Marine Higher Fungi, Brian J. Dyer

Biological Sciences Theses & Dissertations

Four experiments were performed to determine the ability of marine higher fungi to grow in and degrade crude oil. Eight species, previously determined to be hydrocarbonoclastic, were grown in artificial sea water medium (ASM) and southern Louisiana crude oil. The dry mycelial weights were measured and consumption of crude oil was measured gravimetrically. The two species with the best growth, Corollospora maritima and Zalerion maritimum, were grown in ASM containing crude oil spiked with three known hydrocarbons. To this was added glucose (0.15%) and incubated 49 days to test for possible cometabolism of hydrocarbons. Hydrocarbons remaining in the growth …

The Cγ Subunit Is A Unique Isozyme Of The Camp-Dependent Protein Kinase, Stephen J. Beebe, Paul Salomonsky, Tore Jahnsen, Yixin Li

Bioelectrics Publications

There are at least three isozymes (Cα, Cβ, and Cγ) of the mammalian catalytic (C) subunit of cAMP-dependent protein kinase (PKA) (Beebe, S., Oyen, O., Sandberg, M., Froysa, A., Hansson, V., and Jahnsen, T. (1990) Mol. Endocrinol. 4, 465-475). To compare the Cγ and Cα isozymes, the respective cDNAs were expressed in permanently transformed Kin-8 PKA-deficient Y1 adrenal cells using the mouse metallothionein promoter. The recombinant C subunits were characterized as immunoreactive, zinc-inducible, cAMP-dependent kinase activities. In contrast to Cα, histone was a better substrate than Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide) for Cγ. Furthermore, Cγ histone kinase activity was not inhibited by the …

Magnesium Regulation Of Calcium In Essential Hypertension, Elaine Pringle Gordon

Theses and Dissertations in Biomedical Sciences

Essential hypertension is a complex disease which is treated by palliative methods, since the causal factors are as yet unknown. A variety of both genetic and environmental factors probably combine to produce chronic high blood pressure. A genetic model of essential hypertension, spontaneously hypertensive rats (SHR), and its control strain Wistar Kyoto (WKY) were used to investigate a possible environmental causal factor, cellular magnesium deficit, and its impact on the functional ability of the calcium ATPase. Rats were fed either a magnesium-deficient or a magnesium-sufficient diet for 10 weeks, after which the calcium ATPase activity was measured in intact red …

Distribution Of Foraminifera And Pollen In Coastal Depositional Environments Of The Southern Delmarva Peninsula, Virginia, U.S.A., Han Jun Woo

OES Theses and Dissertations

The coastal zone of the southern Delmarva Peninsula exhibits a wide variety of barrier island system subenvironments. This study investigates whether 20 a priori subenvironments can be distinguished from each other on the basis of abiotic environmental variables, pollen assemblages, living foraminiferal populations, and total (living plus dead) foraminiferal assemblages.

The physical data collected from the coastal zone were subjected to canonical variate analysis which discriminated 83% of the stations in 19 groups. These groups were clustered into two internally overlapping sets which represented the inside and outside of the inlet.

Twenty-two pollen types were found in low-energy marsh and …