Digital Commons Network^™

Plasma Processes And Cancer - Special Topical Cluster Of The 2nd Iwpct Meeting, Masaru Hori, Mounir Laroussi, Kai Masur, Yuzuru Ikehara

Electrical & Computer Engineering Faculty Publications

(First paragraph) Although the emerging multidisciplinary field of plasma medicine has been around for nearly two decades important advances have already taken place that could one day revolutionize healthcare and the way various challenging diseases can be treated.1-3Amongst these advances the effects of low temperature plasma (LTP) on cancer cells in vitro and in vivo stand out.4-13Current cancer treatment modalities, such as chemotherapy and radiation therapy, have serious side effects and tend to lose their benefits to the patients after a while. Therefore, novel and improved therapies that can be used alone …

Gadolinium Modifies The Cell Membrane To Inhibit Permeabilization By Nanosecond Electric Pulses, Elena C. Gianulis, Andrei G. Pakhomov

Bioelectrics Publications

Lanthanide ions are the only known blockers of permeabilization by electric pulses of nanosecond duration (nsEP), but the underlying mechanisms are unknown. We employed timed applications of Gd³⁺ before or after nsEP (600-ns, 20 kV/cm) to investigate the mechanism of inhibition, and measured the uptake of the membrane-impermeable YO-PRO-1 (YP) and propidium (Pr) dyes. Gd³⁺ inhibited dye uptake in a concentration-dependent manner. The inhibition of Pr uptake was always about 2-fold stronger. Gd³⁺ was effective when added after nsEP, as well as when it was present during nsEP exposure and removed afterward. Pores formed by nsEP in …

Multi-Scale Visualization Of Molecular Architecture Using Real-Time Ambient Occlusion In Sculptor, Manuel Wahle, Willy Wriggers

Mechanical & Aerospace Engineering Faculty Publications

The modeling of large biomolecular assemblies relies on an efficient rendering of their hierarchical architecture across a wide range of spatial level of detail. We describe a paradigm shift currently under way in computer graphics towards the use of more realistic global illumination models, and we apply the so-called ambient occlusion approach to our opensource multi-scale modeling program, Sculptor. While there are many other higher quality global illumination approaches going all the way up to full GPU-accelerated ray tracing, they do not provide size-specificity of the features they shade. Ambient occlusion is an aspect of global lighting that offers great …

Instructional Guidance In Microblogging-Supported Learning: Insights From A Multiple Case Study, Tian Luo

STEMPS Faculty Publications

Microblogging tools such as Twitter show potential to enrich classroom experience and benefit student learning. Research shows that instructional guidance is particularly necessary in computer-assisted learning environments, but no research has been done to study the effects of instructional guidance in microblogging-based learning. Using a multiple-case study design, the researcher examined student learning in terms of the amount of participation, ability to focus on task, and depth of thinking in guided, semi-guided, and unguided modes. The findings suggest that in guided environments, students achieved higher levels of learning, especially with respect to focusing on task and depth of thinking. Variations …

The Learningweb Revolution And The Transformation Of The School By Leonard J. Waks, Helen Crompton

Teaching & Learning Faculty Publications

On examination of the book’s cover, I believed the images and title would be leading me into reading surface level facts about how technology can be used to support education. I was very wrong. This book was designed to deliver a strong message to all stakeholders in education. Leonard Waks has written this book to present a new type of educational organization as an alternative to the high school system that is in place in the North America today. He clearly states that his ideas do not attempt to fix the broken model, but he strongly advocates for a completely …

The Relationship Between Mobile Learning, Instructional Delivery, And Student Motivation In A Large Undergraduate Science Class, Kristen H. Gregory, Helen Crompton

Teaching & Learning Faculty Publications

Science learning at the early undergraduate level provides a challenging context with large classes and many complex topics to unpack with the students. The purpose of this qualitative study was to explore: how students use mobile devices for learning in a large, undergraduate classroom; what types of instructional delivery could be used with the devices in this context; and if students were motivated to learn. Classroom observations and semi-structured interviews with the professor were reported and five patterns emerged from these data: connected, personal, multimodal, engaged, and class management. From the overall findings of these data, it would appear that …

Dose-Dependent Atp Depletion And Cancer Cell Death Following Calcium Electroporation, Relative Effect Of Calcium Concentration And Electric Field Strength, Emilie Louise Hansen, Esin Bengisu Sozer, Stefania Romeo, Stine Krog Frandsen, P. Thomas Vernier, Julie Gehl

Bioelectrics Publications

Background: Electroporation, a method for increasing the permeability of membranes to ions and small molecules, is used in the clinic with chemotherapeutic drugs for cancer treatment (electrochemotherapy). Electroporation with calcium causes ATP (adenosine triphosphate) depletion and cancer cell death and could be a novel cancer treatment. This study aims at understanding the relationship between applied electric field, calcium concentration, ATP depletion and efficacy. Methods: In three human cell lines — H69 (small-cell lung cancer), SW780 (bladder cancer), and U937 (leukaemia), viability was determined after treatment with 1, 3, or 5 mM calcium and eight 99 μs pulses with 0.8, 1.0, …

Presence And Localization Of Pro-And Mature Forms Of Biglycan And Decorin In Human Costal Cartilage Derived From Chest Wall Deformities, Anthony Asmar, A. Werner, R. E. Kelly Jr., A. Fecteau, Michael W. Stacey

Bioelectrics Publications

Costal cartilage is a type of hyaline cartilage that forms rod-like structures that connect the ribs to the sternum. The most common chest wall deformities, pectus excavatum and pectus carinatum involved efective costal cartilage resulting in sternal displacement. Costal cartilage is not widely studied leaving little insight into possible factors involved in the pathogenesis of these pectus deformities. This study focused on the presence and distribution of two important regulators of collagen fibrillogenesis and organization, biglycan and decorin. Immunohistochemical analysis of transverse cross sections of normal and deformed costal cartilage revealed that biglycan and decorin mainly localized in the territorial …

A Novel Cardiac Muscle-Derived Biomaterial Reduces Dyskinesia And Postinfarct Left Ventricular Remodeling In A Mouse Model Of Myocardial Infarction, Daniel M. O'Connor, Nivedita K. Naresh, Bryan A. Piras, Yaqin Xu, Robert S. Smith, Roy C. Ogle

School of Medical Diagnostics & Translational Sciences Publications

Extracellular matrix (ECM) degradation after myocardial infarction (MI) leaves the myocardium structurally weakened and, as a result, susceptible to early infarct zone dyskinesia and left ventricular (LV) remodeling. While various cellular and biomaterial preparations have been transplanted into the infarct zone in hopes of improving post-MI LV remodeling, an allogeneic cardiac muscle-derived ECM extract has yet to be developed and tested in the setting of reperfused MI. We sought to determine the effects of injecting a novel cardiac muscle-derived ECM into the infarct zone on early dyskinesia and LV remodeling in a mouse model of MI. Cardiac muscle ECM was …

Designing A Broad-Spectrum Integrative Approach For Cancer Prevention And Treatment, Keith I. Block, Charlotte Gyllenhaal, Leroy Lowe, Amedeo Amedei, A.R.M. Ruhul Amin, Amr Amin, Katia Aquilano, Jack Arbiser, Alexandra Arreola, Alla Arzumanyan, Patricia Hentosh

School of Medical Diagnostics & Translational Sciences Publications

Targeted therapies and the consequent adoption of “personalized” oncology have achieved notable successes in some cancers; however, significant problems remain with this approach. Many targeted therapies are highly toxic, costs are extremely high, and most patients experience relapse after a few disease-free months. Relapses arise from genetic heterogeneity in tumors, which harbor therapy-resistant immortalized cells that have adopted alternate and compensatory pathways (i.e., pathways that are not reliant upon the same mechanisms as those which have been targeted). To address these limitations, an international task force of 180 scientists was assembled to explore the concept of a low-toxicity “broad-spectrum” therapeutic …

Bioactive Lipid Coating Of Bone Allografts Direct Engraftment And Fate Determination Of Bone Marrow-Derived Cells In Rat Gfp Chimeras, Anusuya Das, Claire E. Segar, Yihsuan Chu, Tiffany W. Wang, Yon Lin, Chunxi Yang, Roy C. Ogle

School of Medical Diagnostics & Translational Sciences Publications

Bone grafting procedures are performed to treat wounds incurred during wartime trauma, accidents, and tumor resections. Endogenous mechanisms of repair are often insufficient to ensure integration between host and donor bone and subsequent restoration of function. We investigated the role that bone marrow-derived cells play in bone regeneration and sought to increase their contributions by functionalizing bone allografts with bioactive lipid coatings. Polymer-coated allografts were used to locally deliver the immunomodulatory small molecule FTY720 in tibial defects created in rat bone marrow chimeras containing genetically-labeled bone marrow for monitoring cell origin and fate. Donor bone marrow contributed significantly to both …

"Don't Talk To Me About Deception": The Necessary Erosion Of The Trans-Panic Defense, Aimee Wodda, Vanessa R. Panfil

Sociology & Criminal Justice Faculty Publications

[Introduction] A woman sits on a stage and tells a talk show host that a man who has "liked" many of her pictures on Instagram has wanted to meet her for a month, but it had not yet happened because they live in different parts of the country. He called the talk show in order to facilitate their meeting, and she obliged. However, she tells the talk show host: "But he doesn't know my secret." When the host asks her what it is, she replies that she is transgender, to which the audience makes loud "ooooooh" noises. She says that …

Characterization Of Terrestrial Dissolved Organic Matter Fractionated By Ph And Polarity And Their Biological Effects On Plant Growth, Rachel L. Sleighter, Paolo Caricasole, Kristen M. Richards, Terry Hanson, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

Background: Humic substances are ubiquitous in the environment, complex mixtures, and known to be beneficial to plant growth. To better understand and identify components responsible for plant growth stimulation, a terrestrial aquatic DOM sample was fractionated according to pH and polarity, obtaining acid-soluble and acid-insoluble portions, as well as acid-soluble hydrophobic and hydrophilic fractions using C18. The various fractions were characterized then evaluated for their biological effects on plant growth using bioassays with corn at two carbon rates.

Results: Approximately 43% and 57% of the carbon, and 31% and 69% of the iron, was found in the acid-insoluble and acid-soluble …

Modeling Risk For Child Abuse And Harsh Parenting In Families With Depressed And Substance-Abusing Parents, Michelle L. Kelley, Hannah R. Lawrence, Robert J. Milletich, Brittany F. Hollis, James M. Henson

Psychology Faculty Publications

Children with substance abusing parents are at considerable risk for child maltreatment. The current study applied an actor–partner interdependence model to examine how father only (n=52) and dual couple (n=33) substance use disorder, as well as their depressive symptomology influenced parents’ own (actor effects) and the partner's (partner effects) overreactivity in disciplinary interactions with their children, as well as their risk for child maltreatment. Parents completed the Center for Epidemiologic Studies Depression Scale (CES-D; Radloff, 1977), the overreactivity subscale from the Parenting Scale (Arnold, O’Leary, Wolff, & Acker, 1993), and the Brief Child Abuse Potential Inventory (Ondersma, Chaffin, Mullins, & …

Spatio-Temporal Patterns Of Water Table And Vegetation Status Of A Deserted Area, Limin Duan, Tingxi Liu, Xixi Wang, Yanyun Luo

Civil & Environmental Engineering Faculty Publications

Understanding groundwater-vegetation interactions is crucial for sustaining fragile environments of desert areas such as the Horqin Sandy Land (HSL) in northern China. This study examined spatio-temporal variations in the water table and the associated vegetation status of a 9.71 km² area that contains meadowland, sandy dunes, and intermediate transitional zones. The depth of the water table and hydrometeorologic parameters were monitored and Landsat Thematic Mapper (TM) and Moderate Resolution Imaging Spectroradiometer (MODIS) data were utilized to assess the vegetation cover. Spatio-temporal variations over the six-year study period were examined and descriptive groundwater-vegetation associations developed by overlaying a water table …

Identification And Comparative Analysis Of Subolesin/Akirin Ortholog From Ornithodoros Turicata Ticks, Hameeda Sultana, Unnati Patel, Daniel E. Sonenshine, Girish Neelakanta

Biological Sciences Faculty Publications

Background: Subolesin is an evolutionary conserved molecule in diverse arthropod species that play an important role in the regulation of genes involved in immune responses, blood digestion, reproduction and development. In this study, we have identified a subolesin ortholog from soft ticks Ornithodoros turicata, the vector of the relapsing fever spirochete in the United States.

Methods: Uninfected fed or unfed O. turicata ticks were used throughout this study. The subolesin mRNA was amplified by reverse transcription polymerase chain reaction (RT-PCR) and sequenced. Quantitative-real time PCR (QRT-PCR) was performed to evaluate subolesin mRNA levels at different O. turicata developmental stages …

Shortfalls And Solutions For Meeting National And Global Conservation Area Targets, Stuart H. M. Butchart, Martin Clarke, Robert J. Smith, Rachel E. Sykes, Jorn P. W. Scharlemann, Mike Harfoot, Graeme M. Buchanan, Ariadne Angulo, Andrew Balmford, Bastian Bertzky, Thomas M. Brooks, Kent E. Carpenter, 28 Others

Biological Sciences Faculty Publications

Governments have committed to conserving greater than or equal to 17% of terrestrial and greater than or equal to 10% of marine environments globally, especially "areas of particular importance for biodiversity" through "ecologically representative" Protected Area (PA) systems or other "area-based conservation measures", while individual countries have committed to conserve 3-50% of their land area. We estimate that PAs currently cover 14.6% of terrestrial and 2.8% of marine extent, but 59-68% of ecoregions, 77-78% of important sites for biodiversity, and 57% of 25,380 species have inadequate coverage. The existing 19.7 million km super(2) terrestrial PA network needs only 3.3 million …

Quantifying Florida Bay Habitat Suitability For Fishes And Invertebrates Under Climate Change Scenarios, Kelly A. Kearney, Mark J. Butler Iv, Robert Glazer, Christopner R. Kelble, Joseph E. Serafy, Erik Stabenau

Biological Sciences Faculty Publications

The Florida Bay ecosystem supports a number of economically important ecosystem services, including several recreational fisheries, which may be affected by changing salinity and temperature due toclimate change. In this paper, we use a combination of physical models and habitat suitability index models to quantify the effects of potential climate change scenarios on a variety of juvenile fish and lobster species in Florida Bay. The climate scenarios include alterations in sea level, evaporation and precipitation rates, coastal runoff, and water temperature. We find that the changes in habitat suitability vary in both magnitude and direction across the scenarios and species, …

Mutations In The Plasmodium Falciparum Chloroquine Resistance Transporter, Pfcrt, Enlarge The Parasite's Food Vacuole And Alter Drug Sensitivities, Serena Pulcini, Henry M. Staines, Andrew H. Lee, Sarah H. Shafik, Guillaume Bouyer, Catherine M. Moore, Daniel A. Daley, Matthew J. Hoke, Lindsey M. Altenhofen, Heather J. Painter, Jainbing Mu, David J.P. Ferguson, Manuel Llinás, Rowena E. Martin, David A. Fidock, Roland A. Cooper, Sanjeev Krishna

Biological Sciences Faculty Publications

Mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, are the major determinant of chloroquine resistance in this lethal human malaria parasite. Here, we describe P. falciparum lines subjected to selection by amantadine or blasticidin that carry PfCRT mutations (C101F or L272F), causing the development of enlarged food vacuoles. These parasites also have increased sensitivity to chloroquine and some other quinoline antimalarials, but exhibit no or minimal change in sensitivity to artemisinins, when compared with parental strains. A transgenic parasite line expressing the L272F variant of PfCRT confirmed this increased chloroquine sensitivity and enlarged food vacuole phenotype. Furthermore, the introduction …

A Popular And Potentially Sustainable Fishery Resource Under Pressure-Extinction Risk And Conservation Of Brazilian Sciaenidae (Teleostei: Perciformes), Ning L. Chao, Flávia L. Frédou, Manuel Haimovici, Monica B. Peres, Beth Polidoro, Marcelo Raseira, Rosana Subira, Kent E. Carpenter

Biological Sciences Faculty Publications

Croakers (Sciaenidae) are major fishery resource in Brazil; constituting 22% of marine and 9% of freshwater fishery landings. Croakers are subject to heavy fishing pressure throughout Brazil, but habitat alteration is also an important threat to regional populations. In this regional Sciaenidae assessment, each species was analyzed for relative risk of extinction, including the identification and quantification of the impact of major threats and existing conservation measures, based on application of the Categories and Criteria of the IUCN Red List of Threatened Species. Of the 52 species of Sciaenid fishes (34 marine and 18 freshwater) present in Brazilian waters, the …

Dynamic Ocean Management: Defining And Conceptualizing Real-Time Management Of The Ocean, Sara M. Maxwell, Elliot L. Hazen, Rebecca L. Lewison, Danial C. Dunn, Helen Bailey, Steven J. Bograd, Dana K. Briscoe, Sabrina Fossette, Alistair J. Hobday, Meredith Bennett

Biological Sciences Faculty Publications

Most spatial marine management techniques (e.g., marine protected areas) draw stationary boundaries around often mobile marine features, animals, or resource users. While these approaches can work for relatively stationary marine resources, to be most effective marine management must be as fluid in space and time as the resources and users we aim to manage. Instead, a shift towards dynamic ocean management is suggested, defined as management that rapidly changes in space and time in response to changes in the ocean and its users through the integration of near real-time biological, oceanographic, social and/or economic data. Dynamic management can refine the …

Comparative Population Genetics Of Two Invading Ticks: Evidence Of The Ecological Mechanisms Underlying Tick Range Expansions, Robyn Nadolny, Holly Gaff, Jens Carlsson, David Gauthier

Biological Sciences Faculty Publications

Two species of ixodid tick, Ixodes affinis Neumann and Amblyomma maculatum Koch, are simultaneously expanding their ranges throughout the mid-Atlantic region of the US. Although we have some understanding of the ecology and life history of these species, the ecological mechanisms governing where and how new populations establish and persist are unclear. To assess population connectivity and ancestry, we sequenced a fragment of the 16S mitochondrial rRNA gene from a representative sample of individuals of both species from populations throughout the eastern US. We found that despite overlapping host preferences throughout ontogeny, each species exhibited very different genetic and geographic …

Transmission-Blocking Vaccines: Focus On Anti-Vector Vaccines Against Tick-Borne Diseases, Girish Neelakanta, Hameeda Sultana

Biological Sciences Faculty Publications

Tick-borne diseases are a potential threat that account for significant morbidity and mortality in human population worldwide. Vaccines are not available to treat several of the tick-borne diseases. With the emergence and resurgence of several tick-borne diseases, emphasis on the development of transmission-blocking vaccines remains increasing. In this review, we provide a snap shot on some of the potential candidates for the development of anti-vector vaccines (a form of transmission-blocking vaccines) against wide range of hard and soft ticks that include Ixodes, Haemaphysalis, Dermacentor, Amblyomma, Rhipicephalus and Ornithodoros species.

Casitas: A Location-Dependent Ecological Trap For Juvenile Caribbean Spiny Lobsters, Panulirus Argus, Benjamin C. Gutzler, Mark J. Butler Iv, Donald C. Behringer

Biological Sciences Faculty Publications

Casitas are artificial shelters used by fishers to aggregate Caribbean spiny lobsters (Panulirus argus) for ease of capture. However, casitas may function as an ecological trap for juvenile lobsters if they are attracted to casitas and their growth or mortality is poorer compared with natural shelters. We hypothesized that juvenile lobsters may be at particular risk if attracted to casitas because they are less able than larger individuals to defend themselves, and do not forage far from shelter. We compared the nutritional condition, relative mortality, and activity of lobsters of various sizes in casitas and natural shelters in adult and …

Advancing Understanding Of The Role Of Belowground Processes In Terrestrial Carbon Sinks Through Ground-Penetrating Radar: Final Report (De-Sc0008099), Frank P. Day

Biological Sciences Faculty Publications

Coarse roots play a significant role in belowground carbon cycling and will likely play an increasingly crucial role in belowground carbon sequestration as atmospheric CO₂ levels continue to rise, yet they are one of the most difficult ecosystem parameters to quantify. Despite promising results with ground-penetrating radar (GPR) as a nondestructive method of quantifying biomass of coarse roots, this application of GPR is in its infancy and neither the complete potential nor limitations of the technology have been fully evaluated. The primary goals and questions of this study fell into four groups: (1) GPR methods: Can GPR detect change …

An Intersectional Perspective On Stigma As A Barrier To Effective Hiv Self-Management And Treatment For Hiv-Infected African American Women, Kimberly Adams Tufts

Ellmer School of Nursing Faculty Publications

Among those who are HIV-infected and striving to live well with HIV, African American women have poorer health outcomes and represent a higher portion of those women who die from HIV-related causes. Those health inequalities have been associated with the presence of social determinants of health such as stigma. This analytical review asserts that stigmas precipitated by gender, race, and class in the context of HIV-related stigma constitute substantial barriers to active engagement in HIV self-management, care, and treatment for HIV-infected African American women. The utility of the intersectionality framework for analyzing how these various stigmas interact to create unique …

Novel Interventions For Hiv Self-Management In African American Women: A Systematic Review Of Mhealth Interventions, Kimberly Adams Tufts, Kaprea F. Johnson, Jewel Goodman Shepherd, Juyoung Lee, Muna S. Bait Ajzoon, Lauren B. Mahan, Miyoung Kim

Ellmer School of Nursing Faculty Publications

The purpose of this systematic review was to assess the quality of interventions using mobile health (mHealth) technology being developed for and trialed with HIV-infected African American (AA) women. We aimed to assess rigor and to ascertain if these interventions have been expanded to include the broad domain of self-management. After an extensive search using the PRISMA approach and reviewing 450 records (411 published studies and 39 ongoing trials atclinicaltrials.gov), we found little completed research that tested mHealth HIV self-management interventions for AA women. Atclinicaltrials.gov, we found several mHealth HIV intervention studies designed for women in general, forecasting a promising …

De Novo Protein Structure Modeling And Energy Function Design, Lin Chen

Computer Science Theses & Dissertations

The two major challenges in protein structure prediction problems are (1) the lack of an accurate energy function and (2) the lack of an efficient search algorithm. A protein energy function accurately describing the interaction between residues is able to supervise the optimization of a protein conformation, as well as select native or native-like structures from numerous possible conformations. An efficient search algorithm must be able to reduce a conformational space to a reasonable size without missing the native conformation. My PhD research studies focused on these two directions.

A protein energy function—the distance and orientation dependent energy function of …

Influence Of Learner Factors On Soldier Attitude Toward Army Serious Gaming, Mitchell L. Bonnett

STEMPS Theses & Dissertations

This study determined the influence of the learner factors on Soldier attitudes toward the use of serious gaming for U.S. Army training and leader development. It extended Selwyn's work (Selwyn, 1997a, 1997b, 2003, 2004, 2006, 2013; Selwyn, Gorard, Furlong, & Madden, 2003) identifying or measuring attitude toward using a technology and Bonanno and Kommers (2008) work extending Selwyn's work to measure the influence of learner factors on those attitude components toward the use of Army serious gaming for instructional purposes. The population studied was 709 Active duty U.S. Army Soldiers.

This quantitative non-experimental descriptive research design methodology used a 21-item …

An Exploratory Study Of Parenting Dimensions And Family Conflict Among Head Start Participants: An Examination Of Hispanic Mothers, Narketta M. Sparkman-Key, Inglish Morgan-Gardner

Counseling & Human Services Faculty Publications

Understanding the dynamics of the relationship between family conflict and parenting is important to human service practice. When assisting clients, human service practitioners must address many different systems including culture, historical era, ethnicity, gender, and other systems in which the individual operates (Martin, 2013). This study explores this phenomenon by examining the relationship between family conflict and the six dimensions of parenting; warmth, rejection, structure, chaos, autonomy support, and coercion among Hispanic mothers who access an inner city Head Start program. Findings are discussed in terms of cultural impact, human service practice and the need for further research.