Digital Commons Network^™

Delayed Hypersensitivity To Nanosecond Pulsed Electric Field In Electroporated Cells, Sarah D. Jensen, Vera A. Khorokhorina, Claudia Muratori, Andrei G. Pakhomov, Olga N. Pakhomova

Bioelectrics Publications

We demonstrate that conditioning of mammalian cells by electroporation with nanosecond pulsed electric field (nsPEF) facilitates their response to the next nsPEF treatment. The experiments were designed to unambiguously separate the electroporation-induced sensitization and desensitization effects. Electroporation was achieved by bursts of 300-ns, 9 kV/cm pulses (50 Hz, n = 3–100) and quantified by propidium dye uptake within 11 min after the nsPEF exposure. We observed either sensitization to nsPEF or no change (when the conditioning was either too weak or too intense, or when the wait time after conditioning was too short). Within studied limits, conditioning never caused desensitization. …

Caption This: Police In Pussyhats, White Ladies, And Carceral Psychology Under Trump, Alison R. Reed

English Faculty Publications

No abstract provided.

A Synergistic Approach For Evaluating Climate Model Output For Ecological Applications, Rachel D. Cavanagh, Eugene J. Murphy, Thomas J. Bracegirdle, John Turner, Cheryl A. Knowland, Stuart P. Corney, Walker O. Smith Jr., Claire M. Waluda, Nadine M. Johnston, Richard G. J. Bellerby, Eileen E. Hofmann

CCPO Publications

Increasing concern about the impacts of climate change on ecosystems is prompting ecologists and ecosystem managers to seek reliable projections of physical drivers of change. The use of global climate models in ecology is growing, although drawing ecologically meaningful conclusions can be problematic. The expertise required to access and interpret output from climate and earth system models is hampering progress in utilizing them most effectively to determine the wider implications of climate change. To address this issue, we present a joint approach between climate scientists and ecologists that explores key challenges and opportunities for progress. As an exemplar, our focus …

Microbial Invasion Vs. Tick Immune Regulation, Daniel E. Sonenshine, Kevin R. Macaluso

Biological Sciences Faculty Publications

Ticks transmit a greater variety of pathogenic agents that cause disease in humans and animals than any other haematophagous arthropod, including Lyme disease, Rocky Mountain spotted fever, human granulocytic anaplasmosis, babesiosis, tick-borne encephalitis, Crimean Congo haemorhagic fever, and many others (Gulia-Nuss et al., 2016). Although diverse explanations have been proposed to explain their remarkable vectorial capacity, among the most important are their blood feeding habit, their long term off-host survival, the diverse array of bioactive molecules that disrupt the host's natural hemostatic mechanisms, facilitate blood flow, pain inhibitors, and minimize inflammation to prevent immune rejection (Hajdušek et al., 2013). Moreover, …

Interaction Between Air Plasma-Produced Aqueous 1o2 And The Spin Trap Dmpo In Electron Spin Resonance, Chen Chen, Fanying Li, Hai-Lan Chen, Michael G. Kong

Bioelectrics Publications

A series of electron spin resonance (ESR) experiments is done to quantitatively measure the concentrations of aqueous ¹O₂ and OH produced by a surface micro-discharge air plasma device. ¹O₂ is tested to be existed in the plasma treated solution by using the spin trap of TEMP. However, the unexpected DMPOX spectrum is observed in measuringOH by the spin trap of 5,5-Dimethyl-1-Pyrroline-N-Oxide (DMPO). With more chemical scavenger experiments, it is found that removal of aqueous ¹O₂ leads to the disappearance of DMPOX in ESR. Therefore, the generation of DMPOX is directly related to the oxidation …

Enhancing The Teaching And Learning Of Biometeorology In Higher Education, David R. Perkins Iv, Jennifer Vanos, Christopher Fuhrmann, Michael Allen, David Knight, Cameron C. Lee, Angela Lees, Andrew Leung, Rebekah Lucas, Hamed Mehdipor

Political Science & Geography Faculty Publications

Information about the annual meeting organized by the organizations the International Society of Biometeorology (ISB) and the Students and New Professionals (SNP) held in Norfolk, Virginia from July 28 to August 1, 2016 is presented. The event was organized to improve the teaching methods of teachers and learning of students on high education biometeorology and the presentations, practical sessions and group discussions participated by attendees.

State Of The College Address, 2017: College Of Sciences, Gail Dodge

College of Sciences Reports and Documents

PDF of powerpoint presentation for the Old Dominion University State of the College of Sciences address on August 29, 2017.

Impact Of Inpatient Care In Emergency Department On Outcomes: A Quasi-Experimental Cohort Study, Aisha Lateef, Soo-Hoon Lee, Dale Andrew Fisher, Wei-Ping Goh, Hui Fen Han, Uma Chandra Segara, Tiong Beng Sim, Malcolm Mahadehvan, Khean Teik Goh, Noel Cheah, Aymeric Yt Lim, Phillip H. Phan, Reshma A. Merchant

Management Faculty Publications

BACKGROUND: Hospitals around the world are faced with the issue of boarders in emergency department (ED), patients marked for admission but with no available inpatient bed. Boarder status is known to be associated with delayed inpatient care and suboptimal outcomes. A new care delivery system was developed in our institution where boarders received full inpatient care from a designated medical team, acute medical team (AMT), while still residing at ED. The current study examines the impact of this AMT intervention on patient outcomes.

METHODS: We conducted a retrospective quasi-experimental cohort study to analyze outcomes between the AMT intervention and conventional …

Advances In Developing Therapies To Combat Zika Virus: Current Knowledge And Future Perspectives, Ashok Munjal, Rekha Khandia, Kuldeep Dharma, Swati Sachan, Kumaragurubaran Karthik, Ruchi Tiwari, Yashpal S. Malik, Deepak Kumar, Raj K. Singh, Hafiz M. N. Iqbal, Sunil K. Joshi

Bioelectrics Publications

Zika virus (ZIKV) remained largely quiescent for nearly six decades after its first appearance in 1947. ZIKV reappeared after 2007, resulting in a declaration of an international “public health emergency” in 2016 by the World Health Organization (WHO). Until this time, ZIKV was considered to induce only mild illness, but it has now been established as the cause of severe clinical manifestations, including fetal anomalies, neurological problems, and autoimmune disorders. Infection during pregnancy can cause congenital brain abnormalities, including microcephaly and neurological degeneration, and in other cases, Guillain-Barré syndrome, making infections with ZIKV a substantial public health concern. Genomic and …

Distributions, Sources, And Transformations Of Dissolved And Particulate Iron On The Ross Sea Continental Shelf During Summer, Chris M. Marsay, Pamela M. Barrett, Dennis J. Mcgillicuddy Jr., Peter N. Sedwick

OES Faculty Publications

We report water column dissolved iron (dFe) and particulate iron (pFe) concentrations from 50 stations sampled across the Ross Sea during austral summer (January-February) of 2012. Concentrations of dFe and pFe were measured in each of the major Ross Sea water masses, including the Ice Shelf Water and off-shelf Circumpolar Deep Water. Despite significant lateral variations in hydrography, macronutrient depletion, and primary productivity across several different regions on the continental shelf, dFe concentrations were consistently low (<0.1 nM) in surface waters, with only a handful of stations showing elevated concentrations (0.20-0.45 nM) in areas of melting sea ice and near the Franklin Island platform. Across the study region, pFe associated with suspended biogenic material approximately doubled the inventory of bioavailable iron in surface waters. Our data reveal that the majority of the summertime iron inventory in the Ross Sea resides in dense shelf waters, with highest concentrations within 50 m of the seafloor. Higher dFe concentrations near the seafloor are accompanied by an increased contribution to pFe from authigenic and/or scavenged iron. Particulate manganese is also influenced by sediment resuspension near the seafloor but, unlike pFe, is increasingly associated with authigenic material higher in the water column. Together, these results suggest that following depletion of the dFe derived from wintertime convective mixing and sea ice melt, recycling of pFe in the upper water column plays an important role in sustaining the summertime phytoplankton bloom in the Ross Sea polynya.

Optimal Control-Based Inverse Determination Of Electrode Distribution For Electroosmotic Micromixer, Yuan Li, Yongbo Deng, Zhenyu Liu, Teng Zhou, Yihui Wu, Shizhi Qian

Mechanical & Aerospace Engineering Faculty Publications

This paper presents an optimal control-based inverse method used to determine the distribution of the electrodes for the electroosmotic micromixers with external driven flow from the inlet. Based on the optimal control method, one Dirichlet boundary control problem is constructed to inversely find the optimal distribution of the electrodes on the sidewalls of electroosmotic micromixers and achieve the acceptable mixing performance. After solving the boundary control problem, results are also provided to demonstrate the effectiveness of the proposed method; the step-shaped distribution of the external electric potential imposed on the sidewalls is obtained, and the electrodes with an interlaced arrangement …

Supervised Classification Using Finite Mixture Copula, Sumen Sen, Norou Diawara

Mathematics & Statistics Faculty Publications

Use of copula for statistical classification is recent and gaining popularity. For example, statistical classification using copula has been proposed for automatic character recognition, medical diagnostic and most recently in data mining. Classical discrimination rules assume normality. But in this data age time, this assumption is often questionable. In fact features of data could be a mixture of discrete and continues random variables. In this paper, mixture copula densities are used to model class conditional distributions. Such types of densities are useful when the marginal densities of the vector of features are not normally distributed and are of a mixed …

Anaplasma Phagocytophilum Infection Modulates Expression Of Megakaryocyte Cell Cycle Genes Through Phosphatidylinositol-3-Kinase Signaling, Supreet Khanal, Hameeda Sultana, John D. Catravas, Jason A. Carlyon, Girish Neelakanta

Biological Sciences Faculty Publications

Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis infects neutrophils and other cells from hematopoietic origin. Using human megakaryocytic cell line, MEG-01, we show that expression of cell cycle genes in these cells are altered upon A. phagocytophilum infection. Expression of several cell cycle genes in MEG-01 cells was significantly up regulated at early and then down regulated at later stages of A. phagocytophilum infection. Lactate dehydrogenase (LDH) assays revealed reduced cellular cytotoxicity in MEG-01 cells upon A. phagocytophilum infection. The levels of both PI3KCA (p110 alpha, catalytic subunit) and PI3KR1 (p85, regulatory subunit) of Class …

Large Marine Protected Areas Represent Biodiversity Now And Under Climate Change, T. E. Davies, S. M. Maxwell, K. Kaschner, C. Garilao, N. C. Ban

Biological Sciences Faculty Publications

Large marine protected areas (>30,000 km²) have a high profile in marine conservation, yet their contribution to conservation is contested. Assessing the overlap of large marine protected areas with 14,172 species, we found large marine protected areas cover 4.4% of the ocean and at least some portion of the range of 83.3% of the species assessed. Of all species within large marine protected areas, 26.9% had at least 10% of their range represented, and this was projected to increase to 40.1% in 2100. Cumulative impacts were significantly higher within large marine protected areas than outside, refuting the …

Angular Distribution Of Single-Photon Superradiance In A Dilute And Cold Atomic Ensemble, A. S. Kuraptsev, I. M. Sokolov, M. D. Havey

Physics Faculty Publications

On the basis of a quantum microscopic approach we study the dynamics of the afterglow of a dilute Gaussian atomic ensemble excited by pulsed radiation. Taking into account the vector nature of the electromagnetic field we analyze in detail the angular and polarization distribution of single-photon superradiance of such an ensemble. The dependence of the angular distribution of superradiance on the length of the pulse and its carrier frequency as well as on the size and the shape of the atomic clouds is studied. We show that there is substantial dependence of the superradiant emission on the polarization and the …

Designing For Urban Coastal Resilience, Phoebe Crisman

July 28, 2017: The Next Step to Resilience- Projects and Ideas

No abstract provided.

Career Pathways Program Annual Report For Ay 2016/17, Wie Yusuf, Chris Osgood

Career Pathways

It is the mission of the Preparing Future Faculty (PFF) Program at Old Dominion University (ODU) to introduce graduate students and post-doctoral fellows to the diverse roles, the responsibilities, and the rewards of an academic career. In doing so, it focuses particularly on the teaching and service roles sometimes not included in doctoral training. Fundamental research skills, such as grant writing, publishing, and academic job searching, are also addressed.

The PFF program offers a ‘Preparing Future Faculty Certificate’ to graduate students who document completion of specific activities related to preparing for an academic career. Any ODU and NSU degree-seeking graduate …

Circulation, Vol. 22, No. 3, Center For Coastal Physical Oceanography, Old Dominion University, Brynn Davis

CCPO Circulation

Summer 2017 issue of CCPO Circulation featuring article "Graduate School Reflections" by Brynn Davis.

The Relationship Of Early Class Start Times On Sleepiness And Driving Behaviors In An Emerging Adult Population, Jessica L. Fry

Psychology Theses & Dissertations

Teenage driver sleepiness is a recent concern for preventing motor vehicle fatalities. Early school start times limit the amount of sleep teenage high school students acquire during the week and have been related to increased crash risk. The current study extends this finding to teenage and emerging adult college students. The author examined the link between sleepiness and teenage driving behaviors, including the relationship between school start times and sleepiness. In all, 536 participants were recruited to participate in an online survey assessing driving and sleep behaviors. Correlations and path analysis found that sleepiness fully mediated the relationship between early …

When It Is Troublesome To Do Right: A Narrative Analysis Of The Continual Censorship And “Sivilizing” Of Huckleberry Finn, Angela P. Branyon

Teaching & Learning Theses & Dissertations

This qualitative dissertation is a part of a broader program of research that investigates intellectual freedom. The study focuses on developing understanding in three distinct, but related, research areas – the American historical and cultural narrative of race, the historical discourse of intellectual freedom, and the role The Adventures of Huckleberry Finn can play in adding to and perhaps changing these historic stories. By using historical narrative inquiry, data was examined from each story to find correlations among the discourses Where previous research centers on and develops the reasons why Huck Finn has been challenged, this research focuses on how …

The Chondrocyte Channelome: A Novel Ion Channel Candidate In The Pathogenesis Of Pectus Deformities, Anthony J. Asmar

Biological Sciences Theses & Dissertations

Costal cartilage is a type of rod-like hyaline cartilage connecting the ribs to the sternum. The chest wall deformities pectus excavatum (PE) and pectus carinatum (PC) involve displacement of the sternum causing a depression or protrusion of the chest. There is little knowledge about costal cartilage and pectus deformities with much of its understanding based on assumptions from articular cartilage. Chondrocytes are subjected to a constantly changing environment with fluctuations in pH and osmolarity. Ion channels detect these changes and in turn regulate proliferation, differentiation, and extracellular matrix production. Using ion channel qPCR arrays, we produced expression profiles for normal, …

Developing A Career Development Assessment For Predicting Young Stem Graduates’ Employability And Career Barriers, Yi-Ching Lin

STEMPS Theses & Dissertations

The increased concern of declining STEM candidates could negatively impact the U.S. economy (Kelic & Zagnoel, 2009; Maltese & Tai, 2010). Previous studies suggest that the gap between the supply of STEM students in higher education and workface demand is not reflected merely in the number of STEM graduates but instead in the number of qualified STEM graduates who could satisfy STEM workforce demands (Kelic & Zagnoel, 2009; Lowell & Salzman, 2007). The current study used Rae’s employability theory (Rae, 2007) to develop an assessment for evaluating student’s career development in STEM during their higher education. Unlike other instruments focusing …

Finite Element Modeling Driven By Health Care And Aerospace Applications, Fotios Drakopoulos

Computer Science Theses & Dissertations

This thesis concerns the development, analysis, and computer implementation of mesh generation algorithms encountered in finite element modeling in health care and aerospace. The finite element method can reduce a continuous system to a discrete idealization that can be solved in the same manner as a discrete system, provided the continuum is discretized into a finite number of simple geometric shapes (e.g., triangles in two dimensions or tetrahedrons in three dimensions).

In health care, namely anatomic modeling, a discretization of the biological object is essential to compute tissue deformation for physics-based simulations. This thesis proposes an efficient procedure to convert …

Determining Critical Internesting And Foraging Habitats For The Conservation Of Sea Turtles In Gabon, Africa Using Satellite Tracking And Stable Isotope Analysis, Tiffany M. Dawson

Biological Sciences Theses & Dissertations

Understanding the horizontal and vertical habitat of olive ridley sea turtles (Lepidochelys olivacea), a threatened species, is critical for determining regions of protection that may effectively reduce bycatch, the largest threat to this species. Satellite transmitters were used to determine the movement and dive behavior of 21 female olive ridley turtles tagged in Pongara National Park, Gabon during the 2012, 2013, and 2015 nesting seasons. A switching state space model was used to filter the tracking data and categorize the internesting and post-nesting movements. Gridded utilization distribution (UD) home range analysis of tracking data revealed that the entire core habitat …

Unmet Needs And Service Utilization By Informal Caregivers, Ryan Marie Diduk-Smith

Health Services Research Dissertations

The number of Americans expected to live into their 80’s and 90’s is growing at an increased rate in the United States, and the numbers of those aging adults with multiple chronic conditions also continues to grow. It is estimated that by 2050, there will be 89 million adults over the age of 65 years living in the United States. For a variety of reasons, including policy, personal, and financial reasons, many aging adults are choosing to “age in place” or to remain in the community. With the increase in the numbers of aging adults in the community, the ability …

Nanosecond Electric Pulses Differentially Affect Inward And Outward Currents In Patch Clamped Adrenal Chromaffin Cells, Lisha Yang, Gale L. Craviso, P. Thomas Vernier, Indira Chatterjee, Normand Leblanc

Bioelectrics Publications

This study examined the effect of 5 ns electric pulses on macroscopic ionic currents in whole-cell voltage-clamped adrenal chromaffin cells. Current-voltage (I-V) relationships first established that the early peak inward current was primarily composed of a fast voltage-dependent Na⁺ current (I_Na), whereas the late outward current was composed of at least three ionic currents: a voltage-gated Ca²⁺ current (I_Ca), a Ca²⁺-activated K⁺ current (I_K(Ca)), and a sustained voltage-dependent delayed rectifier K⁺ current (I_KV). A constant-voltage step protocol was next used to monitor peak inward and late …

Perspective: The Physics, Diagnostics, And Applications Of Atmospheric Pressure Low Temperature Plasma Sources Used In Plasma Medicine, M. Laroussi

Electrical & Computer Engineering Faculty Publications

Low temperature plasmas have been used in various plasma processing applications for several decades. But it is only in the last thirty years or so that sources generating such plasmas at atmospheric pressure in reliable and stable ways have become more prevalent. First, in the late 1980s, the dielectric barrier discharge was used to generate relatively large volume diffuse plasmas at atmospheric pressure. Then, in the early 2000s, plasma jets that can launch cold plasma plumes in ambient air were developed. Extensive experimental and modeling work was carried out on both methods and much of the physics governing such sources …

Population-Level Coordination Of Pigment Response In Individual Cyanobacterial Cells Under Altered Nitrogen Levels, Jaclyn Murton, Aparna Nagarajan, Amelia Y. Nguyen, Michelle Liberton, Harmony A. Hancock, Himadri B. Pakrasi, Jerilyn A. Timlin

Biological Sciences Faculty Publications

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. We characterized the response of four key photosynthetic pigments to nitrogen depletion and repletion at the subcellular level in individual, live Synechocystis sp. PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. Our results revealed that PBS degradation and re-synthesis comprise a rapid response to …

The Effect Of "Casitas" On Lobster Biology And Fishery Sustainability In The Bahamas, Lester George Gittens

Biological Sciences Theses & Dissertations

“Casitas” (artificial table-like structures) are a commercial fishing gear used to harvest Caribbean spiny lobster (Panulirus argus) in the Caribbean and in The Bahamas, where lobster is the most valuable fishery. Yet, casitas are largely unregulated in The Bahamas and they may threaten fishery sustainability through alteration of lobster growth, disease, or mortality rates and due to insufficient information concerning their number and location. Focusing on the lobster fishery in The Bahamas, my objectives were to: (1) investigate the mortality, growth, and susceptibility to disease of lobsters collected in casitas compared to wooden traps and those living in …

Efficient Algorithms For Solving Size-Shape-Topology Truss Optimization And Shortest Path Problems, Gelareh B. Sanjabi

Civil & Environmental Engineering Theses & Dissertations

Efficient numerical algorithms for solving structural and Shortest Path (SP) problems are proposed and explained in this study. A variant of the Differential Evolution (DE) algorithm for optimal (minimum) design of 2-D and 3-D truss structures is proposed. This proposed DE algorithm can handle size-shape-topology structural optimization. The design variables can be mixed continuous, integer/or discrete values. Constraints are nodal displacement, element stresses and buckling limitations.

For dynamic (time dependent) networks, two additional algorithms are also proposed in this study. A heuristic algorithm to find the departure time (at a specified source node) for a given (or specified) arrival time …