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High-Throughput Single-Molecule Telomere Characterization, Jennifer Mccaffrey, Eleanor Young, Katy Lassahn, Justin Sibert, Steven Pastor, Harold Riethman, Ming Xiao

School of Medical Diagnostics & Translational Sciences Publications

We have developed a novel method that enables global subtelomere and haplotype-resolved analysis of telomere lengths at the single-molecule level. An in vitro CRISPR/Cas9 RNA-directed nickase system directs the specific labeling of human (TTAGGG) n DNA tracts in genomes that have also been barcoded using a separate nickase enzyme that recognizes a 7bp motif genome-wide. High-throughput imaging and analysis of large DNA single molecules from genomes labeled in this fashion using a nanochannel array system permits mapping through subtelomere repeat element (SRE) regions to unique chromosomal DNA while simultaneously measuring the (TTAGGG) n tract length at the end of each …

Motor Learning Guided Treatment For Acquired Apraxia Of Speech: Factors That Influence Treatment Outcomes, Rachel K. Johnson, Joanne P. Lasker, Julie A.G. Stierwalt, Megan K. Macpherson, Leonard L. Lapointe

Communication Disorders & Special Education Faculty Publications

Purpose: The purpose of this study was to examine factors that might influence the treatment effectiveness of motor learning guided (MLG) treatment approach for apraxia of speech (AOS). Specifically, this study examined the effects home practice and the stimuli selection on speech production.

Method: This is a case study across two treatment cycles involving a 52 year-old male five months post left CVA (due to a carotid artery dissection). Each treatment cycle used three conditions of practice to investigate the influence of practice frequency on treatment outcomes. The personal relevance of stimuli within and across treatment conditions differed in the …

Circulation, Vol. 23, No. 1, Center For Coastal Physical Oceanography, Old Dominion University, Bem Hamlington

CCPO Circulation

Fall 2017 issue of CCPO Circulation featuring article "Leading the Way Toward Better Projections of Regional Sea Level Rise" by Ben Hamlington.

Activation Of The Phospholipid Scramblase Tmem16f By Nanosecond Pulsed Electric Field (Nspef) Facilitates Its Diverse Cytophysiological Effects, Claudia Muratori, Andrei G. Pakhomov, Elena Gianulis, Jade Meads, Maura Casciola, Peter A. Mollica, Olga N. Pakhomova

Bioelectrics Publications

Nanosecond pulsed electric fields (nsPEF) are emerging as a novel modality for cell stimulation and tissue ablation. However, the downstream protein effectors responsible for nsPEF bioeffects remain to be established. Here we demonstrate that nsPEF activate TMEM16F (or Anoctamin 6), a protein functioning as a Ca²⁺-dependent phospholipid scramblase and Ca²⁺-activated chloride channel. Using confocal microscopy and patch clamp recordings, we investigated the relevance of TMEM16F activation for several bioeffects triggered by nsPEF, including phosphatidylserine (PS) externalization, nanopore-conducted currents, membrane blebbing, and cell death. In HEK 293 cells treated with a single 300-ns pulse of 25.5 kV/cm, …

Microstructure Of Attachment Mechanisms Of Newly Hatched Larvae Of Four Cyprinid Species With Comments On Terminology, George E. Maurakis, Eugene G. Maurakis

Virginia Journal of Science

An adhesive organ is a prominent, protruding mucus secreting gland that is used by newly hatched tadpoles and larvae of some fishes to attach to aquatic vegetation.  The objective of this research is to test the hypothesis that newly hatched cyprinid larvae of Hybognathus hankinsoni, Notemigonus crysoleucas, Cyprinus carpio and Gila atraria contain cephalic adhesive organs.  Newly hatched larvae of Semotilus atromaculatus, which do not attach to submerged aquatic vegetation, were used as the control.  SEM examination of newly hatched larvae indicate there were no adhesive organs on the control species (S. atromaculatus) or test species …

Habitat Use By Bats On The Upper Coastal Plain Of Virginia, A. Scott Bellows, Joseph C. Mitchell

Virginia Journal of Science

We monitored bat activity on the upper Coastal Plain of Virginia using mist nets and acoustic detection (ANABAT) during April-October 2000 and April-August 2001. We classified forty sites into three forest-cover classes (pine forest, mixed pine, and hardwood forest) and three landscape-feature classes (permanent water, riparian corridor, and upland). We captured 406 bats (8 species) in mist nets; red bats (Lasiurus borealis; n = 281), big brown bats (Eptesicus fuscus; n = 47), and eastern pipistrelles (Pipistrellus subflavus; n = 36) were the most commonly captured species. We captured fewer than 30 individuals of …

Predation On Artificial Turkey Nests At Radford Army Ammunition Plant In Western Virginia, Shane Brandes, Karen E. Powers, Len L. Diioia Jr.

Virginia Journal of Science

Because ground-nesting wild turkeys (Meleagris gallopavo) may sustain high incidences of nest predation in western Virginia, determining their predators is essential to understanding risk and managing the birds.  Our study investigated potential predators of wild turkey nests at the Radford Army Ammunition Plant, New River Unit (RFAAP; Pulaski Co., in western Virginia). Here, we established 8 artificial nests during the breeding season for wild turkey (March-April, 2017), and documented predators via game cameras.  Thirty-one species of mammals and birds visited the nests over the 31-day study. Nest predation was verified 56 times across 6 species, including coyotes ( …

A Near-Global Atmospheric Distribution Of N2o Isotopologues, Peter F. Bernath, Mahdi Yousefi, Eric Buzan, Chris D. Boone

Chemistry & Biochemistry Faculty Publications

The distributions of the four most abundant isotopologues and isotopomers (N₂O, ¹⁵NNO, N¹⁵NO, and NN¹⁸O of nitrous oxide have been measured in the Earth's stratosphere by infrared remote sensing with the Atmospheric Chemistry Experiment (ACE) Fourier transform spectrometer. These satellite observations have provided a near-global picture of N₂O isotopic fractionation. The relative abundances of the heavier species increase with altitude and with latitude in the stratosphere as the air becomes older. The heavy isotopologues are enriched by 20-30% in the upper stratosphere and even more over the poles. These observations are …

Effects Of Vacuuming Pervious Concrete On Infiltration Rate, Demetrios E. Maurakis, Eugene G. Maurakis

Virginia Journal of Science

The objectives of this research were twofold: test the infiltration rate of pervious concrete before and after vacuum cleaning and assess infiltration variability over time. Infiltration tests were performed on a 558.2 m2 area of pervious concrete, divided into sixteen 0.35 m2 test areas in a parking area at the Science Museum of Virginia in Richmond, Virginia on five dates from November 4-December 30, 2013. Average infiltration rates (avg.= 22.8 and 36.3 L/m²/min) immediately after vacuuming were lower than those (avg.=30.7 and 41.3 L/m²/min) before vacuuming. We hypothesize that the vacuuming lifted materials towards the surface …

The Effects Of Climate On Radial Growth Of Disjunct Northern White Cedar (Thuja Occidentalis L.) In Virginia, Joshua A. Kincaid

Virginia Journal of Science

Understanding the geographic range and growth of species is essential for effective land management in a landscape affected by anthropogenic activity and climate change.  Climate change is expected to alter the distribution and growth of many tree species in eastern North America, including northern white cedar (Thuja occidentalis L.).  This research examined the effects of climate on radial growth of T. occidentalis in disjunct populations south of its continuous range margin in eastern North America.  A T. occidentalis tree-ring chronology was developed and examined for growth-climate interactions. Mean sensitivity of the T. occidentalis chronology was within the range of …

A Social-Ecological Framework Of Theory, Assessment, And Prevention Of Suicide, Robert J. Cramer, Nester D, Kapusta

Community & Environmental Health Faculty Publications

The juxtaposition of increasing suicide rates with continued calls for suicide prevention efforts begs for new approaches. Grounded in the Centers for Disease Control and Prevention (CDC) framework for tackling health issues, this personal views work integrates relevant suicide risk/protective factor, assessment, and intervention/prevention literatures. Based on these components of suicide risk, we articulate a Social-Ecological Suicide Prevention Model (SESPM) which provides an integration of general and population-specific risk and protective factors. We also use this multi-level perspective to provide a structured approach to understanding current theories and intervention/prevention efforts concerning suicide. Following similar multi-level prevention efforts in interpersonal violence …

Healthcare Outcomes And Resource Utilization Associated With Neonatal Hypoglycemia: Analysis Of Data From The Hcup Kid’S Inpatient Database, Brook T. Alemu

Health Services Research Dissertations

Neonatal hypoglycemia is the most common metabolic abnormality in infants and is associated with neurological damage and death. The risk of developing hypoglycemia among infants born from diabetic mothers is even higher. Although much work has been performed addressing issues for treatment and care, research related to neonatal hypoglycemia has been focused on the clinical or individual level risk factors. Contextual risk factors such as hospital characteristics, neighborhood economic status, and regional variations were not considered in earlier studies. Additionally, although healthcare resources utilization of hypoglycemia has been adequately addressed in the adult population, this topic has not been studied …

Quantifying Pollutant Removal Rates Of Bioretention Basins As A Stormwater Best Management Practice, Evan Nathanial Waagen

Civil & Environmental Engineering Theses & Dissertations

Water pollution is an ongoing problem that can be attributed to human activities. As world population increases and countries become more developed, this problem intensifies. Fortunately, the causes and solutions of water pollution are documented and have been implemented with various levels of success. These solutions, or Best Management Practices (BMPs), vary in type and function and remove pollutants from runoff prior to it reaching rivers, lakes, and other bodies of water. This study investigates bioretention basins, a specific group of BMPs, and presents analysis and prediction of their performance, of which our knowledge is incomplete in the existing literature. …

Marine Reserves Promote Coral Reef Resilience By Mitigating Human Impacts Through The Restoration Of Parrotfish Populations, Increasing Their Reproductive Output And Seeding Neighboring Overfished Reefs, Brian L. Stockwell

Biological Sciences Theses & Dissertations

Coral reefs are some of the most productive ecosystems on the planet, providing fisheries resources for over a billion people with billions of dollars in revenue from tourism for developing nations. Coral reefs are under threat from overfishing and water pollution, resulting in less productive algae dominated reefs. Marine reserves are widely expected to promote the resilience of reefs by protecting and increasing the abundance of herbivorous fishes that can graze on algae, therefore directly or indirectly preventing coral to algal phase shifts. However, the ability of marine reserves to mitigate human impacts, restore herbivorous fish populations and seed nearby …

Using Optimality Theory And Reference Points To Improve The Diversity And Convergence Of A Fuzzy-Adaptive Multi-Objective Particle Swarm Optimizer, Amit A. Kulkarni

Mechanical & Aerospace Engineering Theses & Dissertations

Particle Swarm Optimization (PSO) has received increasing attention from the evolutionary optimization research community in the last twenty years. PSO is a metaheuristic approach based on collective intelligence obtained by emulating the swarming behavior of bees. A number of multi-objective variants of the original PSO algorithm that extend its applicability to optimization problems with conflicting objectives have also been developed; these multi-objective PSO (MOPSO) algorithms demonstrate comparable performance to other state-of-the-art metaheuristics. The existence of multiple optimal solutions (Pareto-optimal set) in optimization problems with conflicting objectives is not the only challenge posed to an optimizer, as the latter needs to …

Human Rickettsial Pathogen Modulates Arthropod Organic Anion Transporting Polypeptide And Tryptophan Pathway For Its Survival In Ticks, Vikas Taank, Shovan Dutta, Amrita Dasgupta, Durland Fish, John F. Anderson, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

The black-legged tick Ixodes scapularis transmits the human anaplasmosis agent, Anaplasma phagocytophilum. In this study, we show that A. phagocytophilum specifically up-regulates I. scapularis organic anion transporting polypeptide, isoatp4056 and kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), for its survival in ticks. RNAi analysis revealed that knockdown of isoatp4056 expression had no effect on A. phagocytophilum acquisition from the murine host but affected the bacterial survival in tick cells. Knockdown of the expression of kat mRNA alone or in combination with isoatp4056 mRNA significantly affected A. phagocytophilum survival …

Thermally Assisted Pulsed Electric Field Ablation For Cancer Therapy, James Michael Hornef

Biomedical Engineering Theses & Dissertations

Pulsed Electric Fields (PEF) have promised improved treatment results in a variety of cancer types including melanoma, pancreatic and lung squamous cancer. Recent studies show that PEF-based cancer therapy may be improved further with the assistance of moderate heating of the target. Experiments were performed to design, calibrate and implement a feedback-looped infrared laser irradiation system that could maintain specified temperatures during the treatment. The exact treatment area, penetration depth and thermal distribution of a 980-nm laser fiber were quantified using several methods, including the knife-edge technique and a tissue optical property study. In vivo and in vitro experiments using …

Biology And Impacts Of Leech Infestation In Largemouth Bass (Micropterus Salmoides) In Back Bay, Virginia, Amanda Nicole Pomposini

Biological Sciences Theses & Dissertations

Back Bay is an oligohaline coastal bay in Southeast Virginia, USA. The Oregon Inlet along the Outer Banks of North Carolina is the only significant salinity source to the Bay via the Pamlico and Currituck Sounds of North Carolina. Today Back Bay is considered a brackish ecosystem with salinity of 2004, leeches have been observed in the oral cavities of Largemouth Bass (Micropterus salmoides) in Back Bay. Leeches (Myzobdella lugubris) have previously been documented in the oral cavities of largemouth bass in the Currituck Sound. In 2009, the Virginia Department of Game and Inland Fisheries (VDGIF) …

Infusing Complex Problem-Solving And Systems Thinking In College Leadership Development Programs, Dionicia Mahler-Rogers

Educational Leadership & Workforce Development Theses & Dissertations

Present day leaders grapple with a dynamic environment of evolving challenges. Prior leadership studies have afforded revisions of leadership theories in an attempt to remain relevant with the prominently shifting environment. The leadership models that underscore college leadership development programs should reflect the updated theories and practices which expose student leaders to solving complex problems they will experience. This study explores the knowledge, skills, and abilities needed for college students to be able to conceptualize and address system’s level leadership challenges. Foremost, the study examined instructional tools and methods that students found impactful for improving conceptualization and achievement of systems-level …

Population Structure Of Lethrinus Lentjan (Lethrinidae, Percoidei) Across The South China Sea And The Philippines Is Detected With Lane-Affected Radseq Data, Ellen E. Biesack

Biological Sciences Theses & Dissertations

Southeast Asia includes the Coral Triangle, a marine biodiversity hotspot that supports important fishery resources experiencing varied threats. Patterns of speciation and population structure in the Coral Triangle have been examined to test hypotheses relating to the historical geologic processes that may have influenced this biodiversity phenomenon. This study investigates the genetic population structure of the Pink-ear Emperor Snapper, Lethrinus lentjan (Lacepède, 1802), across the Philippines and the South China Sea. The species is fished throughout the Coral Triangle by subsistence and commercial fishers and their landings have been in decline for several years, which could be indicative of depleted …

Lifespan Communication And Career Development Of Black Teachers: A Socio-Ecological Approach, Veronica Whinnett Hurd

Communication & Theatre Arts Theses

This thesis unlocks the lifespan story of nine Black participants as they reflected on the communicative practices that guided their career journey towards becoming a teacher. Through the lens of Bronfenbrenner’s (1979) socio-ecological development model, the microsystem, mesosystem, exosystem, macrosystem, and chronosystem were examined to understand the content of career-related memories and with whom or what the communicative experiences occurred with across the participants’ lifespan. This study also takes an in-depth look at how the content of the memories evolved across Erikson’s (1964) childhood, adolescence, and young adulthood developmental periods, and the influence of the proximal and distal socio-ecological environments …

Selenium Distribution And Cycling In The Eastern Equatorial Pacific Ocean, Zoe Wambaugh

OES Theses and Dissertations

Oxygen minimum zones in oceanic waters have become increasingly important to the marine environment and society. Low oxygen waters affect not only the distribution and abundance of marine organisms, but also impact the solubility and transport of trace elements that are of biological importance, with the chemical speciation and solubility depending on the actual redox poise of the waters. One redox sensitive trace element of interest is selenium, which can be both toxic and essential for organisms, depending on its chemical speciation. In 2013, the US GEOTRACES program completed the GP16 transect from Peru to Tahiti, going through the oxygen …

Tracing The Genetic Footprints Of The Redbelly Yellowtail Fusilier, Caesio Cuning, Across Multiple Spatial And Evolutionary Scales, Amanda Susanne Ackiss

Biological Sciences Theses & Dissertations

Overfishing is one of the most pervasive threats to coral reef ecosystems, and management of these multi-species resources is hampered by limited species-specific population level information. The reefs in the western tropical Pacific Ocean, including the Coral Triangle, are the most bio-diverse in the world. Home to more than 400 million people, this region contains some of the most threatened coral reef ecosystems. Presented here is the first comprehensive analysis of the genetic structure of Caesio cuning, planktivorous fish inhabiting reefs in the Coral Triangle and western Pacific Ocean. Data from both classical Sanger and next-generation sequencing were analyzed …

Efficient Machine Learning Approach For Optimizing Scientific Computing Applications On Emerging Hpc Architectures, Kamesh Arumugam Karunanithi

Computer Science Theses & Dissertations

Efficient parallel implementations of scientific applications on multi-core CPUs with accelerators such as GPUs and Xeon Phis is challenging. This requires - exploiting the data parallel architecture of the accelerator along with the vector pipelines of modern x86 CPU architectures, load balancing, and efficient memory transfer between different devices. It is relatively easy to meet these requirements for highly-structured scientific applications. In contrast, a number of scientific and engineering applications are unstructured. Getting performance on accelerators for these applications is extremely challenging because many of these applications employ irregular algorithms which exhibit data-dependent control-flow and irregular memory accesses. Furthermore, these …

Synthesis, Characterization And Application Of Sugar-Based Low Molecular Weight Gelators, Ifeanyi Simeon Okafor

Chemistry & Biochemistry Theses & Dissertations

Low molecular weight gelators (LMWGs) are an interesting class of compound that have gained considerable attention especially because of their potential applications for soft biomaterials. Carbohydrate based low molecular weight hydrogelators and organogelators are able to selfassemble and form ordered supramolecular structures, which are useful for exploring biomedical applications. The resulting gelators are responsive to external stimuli because of the weak noncovalent intermolecular forces and interaction that influences self-assembly. Stimuli-responsive supramolecular organogels with interesting properties in response to external environmental stimuli have gained considerable attention due to their applications in biomaterials, sensors and for drug delivery.

One of the major …

Effects Of Carboxylated Nanodiamonds On Macrophages During And After Differentiation, Maisoun E. Bani Hani

Biological Sciences Theses & Dissertations

Nanodiamonds (ND) are a carbon-based nanomaterial that are increasingly being proposed for developing novel imaging techniques, as carriers of biomolecules and therapeutic drugs, as coatings for implants, and for other biomedical applications. The exceptional chemical, mechanical, and optical properties of ND make this material suitable in a wide range of fields. The application of ND in the biomedical field is attractive but requires more in-depth investigation into the safety of ND and its interactions with different cells and systems. The effects of ND on the immune system are not fully understood or investigated and there are several controverting reports regarding …

Life History And Management Methods For Blueline Tilefish (Caulolatilus Microps) From The United States Mid-Atlantic Region, Michael A. Schmidtke

OES Theses and Dissertations

Blueline tilefish (Caulolatilus microps) have recently undergone an increase in landings from waters off the US Mid-Atlantic region (Virginia-Massachusetts). Currently, life history characteristics of blueline tilefish in this area are undescribed. Additionally, appropriate indices of population abundance are not available for this region, necessitating the use of data-limited management methods to set restrictions for harvest of blueline tilefish in this area. This dissertation describes growth and reproductive characteristics of blueline tilefish caught off the coast of Virginia and explores improvements of data-limited management methods that have been applied to blueline tilefish in the US Mid-Atlantic. Blueline tilefish from …

Is Every Tweet Created Equal? A Framework To Identify Relevant Tweets For Business Research, Thad Chee

Information Technology & Decision Sciences Theses & Dissertations

It is a life or death matter for a firm to observe its environment and identify new threats or opportunities quickly. Information technology has increased firm’s speed and agility in responding to environmental changes. Social media offers a vast and timely source of environmental information that firms can readily use gauge public sentiment. Twitter is a high-speed service that allows anyone to “tweet” a message to any interested parties. Firms can access near instantaneous changes in the public mood about any topic by using Sentiment Analysis. These topics range from predicting equities prices to predicting election outcomes. A gap exists …

Exploring The Relationship Between Media Influence And Policy Output: A State Comparative Study, Stephanie Savas Joannou Menefee

School of Public Service Theses & Dissertations

Through the years, various forms of media have become political institutions able to influence the public policy agenda. Analyzing policy helps to promote a better understanding of how the policy process works, how problems appear on the political agenda, and why governments choose to act or not to act on particular policy problems. This research utilizes Baumgartner and Jones’s (1993) Theory of Punctuated Equilibrium (PET) to examine the ways in which media attention influenced public policy on marijuana in the states of Kentucky, Massachusetts, and Vermont between 1996 and 2016.

Using qualitative research methods, the researcher conducted an exploratory, comparative …

Graduate News, Graduate School, Old Dominion University

Graduate News

September 2017 issue of Graduate News from the ODU Graduate School.