Digital Commons Network^™

Identifying Marine Key Biodiversity Areas In The Greater Caribbean Region, Michael S. Harvey

Biological Sciences Theses & Dissertations

Anthropocene biodiversity extinction rates are increasing, suggesting a possible sixth global mass extinction event. Biological conservation planners are consequently seeking ways to more effectively protect species at national, regional and global scales. In 2010, the Convention on Biological Diversity (CBD) issued a number of conservation goals (Aichi Targets), including the establishment of protected areas (PA) in terrestrial, freshwater and marine areas of eminent conservation concern by 2020 to reduce and eventually eliminate species’ extinctions, as well as preserve hotspots of biodiversity and dynamic ecosystems. While well-established, adequately enforced PAs increase the likelihood of preserving species and habitats most at risk …

Examining The Association Of Environmental Degradation And Poor Cardiovascular And/Or Respiratory Health Outcomes In A Disadvantaged Community, Khyati Niranjan Kantaria

Health Services Research Dissertations

Air pollution is associated with poor cardiovascular and/or respiratory health outcomes. The poor air quality in certain communities due to the emission of toxic air pollutants from industries and major roadways has been a growing concern. The main objective of this study was to examine whether residential proximity to environmental air pollution sources, individual-level risk factors (age, gender, and body mass index (BMI)) and number of years at same residence are associated with observed poor cardiovascular and/or respiratory health outcomes in the residents of Southeast community in Newport News, Virginia. Logistic regression was conducted to assess this association using the …

Biotime: A Database Of Biodiversity Time Series For The Anthropocene, Maria Dornelas, Laura H. Antão, Faye Moyes, Amanda E. Bates, Anne E. Magurran, Dušan Adams, Asem A. Akhmetzhanova, Ward Appeltans, José Manuel Adam, Frank P. Day

Biological Sciences Faculty Publications

Motivation: The BioTIME database contains raw data on species identities and abundances in ecological assemblages through time. These data enable users to calculate temporal trends in biodiversity within and amongst assemblages using a broad range of metrics. BioTIME is being developed as a community-led open-source database of biodiversity time series. Our goal is to accelerate and facilitate quantitative analysis of temporal patterns of biodiversity in the Anthropocene.

Main types of variables included: The database contains 8,777,413 species abundance records, from assemblages consistently sampled for a minimum of 2 years, which need not necessarily be consecutive. In addition, the database contains …

A Survey Of Matrix Completion Methods For Recommendation Systems, Andy Ramlatchan, Mengyun Yang, Quan Liu, Min Li, Jianxin Wang, Yaohang Li

Computer Science Faculty Publications

In recent years, the recommendation systems have become increasingly popular and have been used in a broad variety of applications. Here, we investigate the matrix completion techniques for the recommendation systems that are based on collaborative filtering. The collaborative filtering problem can be viewed as predicting the favorability of a user with respect to new items of commodities. When a rating matrix is constructed with users as rows, items as columns, and entries as ratings, the collaborative filtering problem can then be modeled as a matrix completion problem by filling out the unknown elements in the rating matrix. This article …

The Importance Of Keeping The Big Ones: Harvest Slot Limits And Marine Protected Areas For The Management Of The Caribbean Spiny Lobster, Gayathiri Gnanalingam

Biological Sciences Theses & Dissertations

Fishing typically removes the oldest and/or largest individuals from populations undermining stability and reproductive success. Traditional fisheries management tools fail to protect these oldest and/or largest individuals, but two less conventional tools: marine protected areas (MPAs), and harvest slot limits have the potential to do so. Here I tested the possible use of these tools for the Caribbean spiny lobster, Panulirus argus, an iconic and economically valued species. After decades of intense fishing, the largest lobsters have largely been wiped out. The loss of the largest lobsters is significant as large lobsters have considerably greater reproductive potential than their …

Generation Of Large-Volume Diffuse Plasma By An External Ionization Wave From A Single-Electrode Plasma Jet, Seyed Hamid Razavi Barzoki

Electrical & Computer Engineering Theses & Dissertations

A non-thermal transient diffuse plasma can be generated remotely in a nonconductive reduced pressure chamber by an external guided fast ionization wave (FIW). We found that an atmospheric-pressure low-temperature plasma jet (APPJ) can be a source of FIW which transfers an enhanced electric field at the wave front across a reduced pressure Pyrex glass chamber with no electrical connection to the chamber. Here, we studied the formation and propagation of the APPJ plasma, the interaction of atmospheric-pressure guided FIW with a dielectric surface which forms the wall of the reduced-pressure system, and the formation and propagation of the reduce-pressure FIW …

Local Adaptation Signatures In Thermal Performance Of The Temperate Coral Astrangia Poculata, Hannah Elise Aichelman

Biological Sciences Theses & Dissertations

The Northern Star Coral (Astrangia poculata) is an understudied temperate scleractinian coral that provides unique opportunities to understand the roles of phenotypic plasticity and local adaptation in coral physiological tolerance limits. Astrangia poculata inhabits hard bottom ecosystems from the northwestern Atlantic to the Gulf of Mexico and withstands an annual temperature range up to 20°C. Additionally, A. poculata is facultatively symbiotic and co-occurs in both symbiotic (“brown”) and aposymbiotic (“white”) states. Here, brown and white A. poculata were collected from Virginia (VA) and Rhode Island (RI), USA and exposed to heat (18-32°C) and cold (18-6°C) temperature assays during …

Plastics And Microplastics As Vectors For Bacteria And Human Pathogens, Amanda Lee Laverty

OES Theses and Dissertations

Since plastics degrade very slowly, they remain in the environment on much longer timescales than most natural substrates and can thus provide a novel habitat for colonization by bacterial communities. The full spectrum of relationships between plastics and bacteria, however, is little understood. The objective of this study was to examine marine plastic pollution as a substrate for bacteria, with particular focus on Vibrio spp., including the human pathogens, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.

Colonization experiments were set up in a tributary of the lower Chesapeake Bay to follow Vibrio spp. colonization and …

Potential Interaction Analysis Of Offshore Wind Energy Areas And Breeding Avian Species On The Us Mid-Atlantic Coast, Jeri Lynn Wisman

Biological Sciences Theses & Dissertations

Due to increasing US interest in developing wind energy sites in offshore waters, we synthesized existing data on colonial breeding seabird populations with the potential risk of interacting with lease areas in the mid-Atlantic. Previous efforts by BOEM and NOAA have predicted avian density using at-sea survey data; we seek to complement this work by focusing specifically on birds during the critical and energetically demanding breeding life history stage. We combined colony size and location for each species along the mid-Atlantic coast with buffers around the colonies that correlate with the species’ foraging range. We integrated population size, vulnerability to …

Characterizing The Activity Of Antimicrobial Peptides Against The Pathogenic Bacterium Clostridium Difficile In An Anaerobic Environment, Adenrele Mojeed Oludiran

Chemistry & Biochemistry Theses & Dissertations

Clostridium difficile is an anaerobic Gram-positive pathogen with high treatment costs and mortality and very high antibiotic tolerance. Antimicrobial host-defense peptides (HDPs) produced naturally by animal immune systems are promising candidates to develop novel therapies for bacterial infection because they cause oxidative stress that damages multiple targets in bacterial cells, so it is difficult for bacteria to evolve resistance to these attacks.

Piscidins, fish-derived HDPs that can also form complexes with copper (Cu) to enhance their activities, are very active against multiple bacterial species in an aerobic environment. We examined their activity against C. difficile and other species in an …

Developmental Steps For A Functional Three-Dimensional Cell Culture System For The Study Of Asymmetrical Division Of Neural Stem Cells, Martina Zamponi

Biomedical Engineering Theses & Dissertations

Stem cells are a cell type present during and following development, which possess self- renewal properties, as well as the ability to differentiate into specific cells. Asymmetrical division is the cellular process that allows stem cells to produce one differentiated and one un-differentiated daughter cell during the same mitotic event. Insights in the molecular mechanisms of such process are minimal, due to the absence of effective methods for its targeted study. Currently, traditional methods of investigation include monolayer cell culture and animal models. The first poses structural limitations to the accurate representation of human tissue and cell structures, while animal …

Deep Learning For Segmentation Of 3d Cryo-Em Images, Devin Reid Haslam

Computer Science Theses & Dissertations

Cryo-electron microscopy (cryo-EM) is an emerging biophysical technique for structural determination of protein complexes. However, accurate detection of secondary structures is still challenging when cryo-EM density maps are at medium resolutions (5-10 Å). Most existing methods are image processing methods that do not fully utilize available images in the cryo-EM database. In this paper, we present a deep learning approach to segment secondary structure elements as helices and β-sheets from medium- resolution density maps. The proposed 3D convolutional neural network is shown to detect secondary structure locations with an F1 score between 0.79 and 0.88 for six simulated test cases. …

The Effect Of Soxhlet Extraction And Synthesis Temperature On Properties Of Polyaniline, Samuel Watson Stahl

Chemistry & Biochemistry Theses & Dissertations

Polyaniline was synthesized by the chemical oxidation of aniline in an HCl solution by ammonium persulfate. The temperature of the synthesis was varied to increase the molecular weight of the polymer from 37,600 Da to 52,400 Da. Soxhlet extraction using methanol as the primary solvent was performed on half the sample in order to further increase the average molecular weight by approximately 3150 Da and decrease the polydispersity index by 0.8. Films were cast from 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone (DMPU) and N-methyl-2-pyrrolidone (NMP) and then doped with trifluoromethanesulfonic acid.

The effect of the soxhlet extraction and synthesis temperature on the polymer’s properties …

Advances In Amino Acid Analysis For Marine Related Matrices And Its Application To Coastal Shelf Settings In The Canadian Arctic, Rachel M. Mcmahon

OES Theses and Dissertations

Amino acids comprise up to 50% of organic matter in cellular material and are a major fraction of oceanic organic carbon. Amino acids are also considered highly labile during organic matter recycling, making them useful proxies for organic carbon cycling. Nevertheless, analysis of individual amino acids has been burdened by lengthy derivatization and complex analysis since the 1950s. In this thesis, I describe the modification of advanced analytical techniques, developed in the biomedical field, for analysis of marine matrices which allow the determination of at least 40 amino acids without the need for lengthy sample preparation and derivatization, twice the …

A Multi Case Study Of Community College Discipline Faculty’S Participation In A Disciplinary Literacy Professional Learning Community, Kristen Howell Gregory

Teaching & Learning Theses & Dissertations

Many students enter college with inadequate reading, writing, and critical thinking skills to successfully navigate discipline-specific college-level coursework (Duff, 2010; Hyland, 2006; Lea & Street, 1998; Tsui, 2002). As such, college faculty, and specifically community college faculty, are challenged to meet the multiple literacy needs of their students while still maintaining high expectations within their discipline-specific courses. One option is for discipline faculty (e.g., history) to integrate disciplinary literacy instruction within their courses. As discipline faculty are deemed experts in their content area and often not trained in literacy, professional development focused on disciplinary literacy could provide the knowledge and …

Extended Poisson Models For Count Data With Inflated Frequencies, Monika Arora

Mathematics & Statistics Theses & Dissertations

Count data often exhibits inflated counts for zero. There are numerous papers in the literature that show how to fit Poisson regression models that account for the zero inflation. However, in many situations the frequencies of zero and of some other value k tends to be higher than the Poisson model can fit appropriately. Recently, Sheth-Chandra (2011), Lin and Tsai (2012) introduced a mixture model to account for the inflated frequencies of zero and k. In this dissertation, we study basic properties of this mixture model and parameter estimation for grouped and ungrouped data. Using stochastic representation we show …

Sequence-Based Simulation-Optimization Framework With Application To Port Operations At Multimodal Container Terminals, Mariam Aladdin Kotachi

Engineering Management & Systems Engineering Theses & Dissertations

It is evident in previous works that operations research and mathematical algorithms can provide optimal or near-optimal solutions, whereas simulation models can aid in predicting and studying the behavior of systems over time and monitor performance under stochastic and uncertain circumstances. Given the intensive computational effort that simulation optimization methods impose, especially for large and complex systems like container terminals, a favorable approach is to reduce the search space to decrease the amount of computation.

A maritime port can consist of multiple terminals with specific functionalities and specialized equipment. A container terminal is one of several facilities in a port …

Illuminator, Volume 1, Issue 9, Batten College Of Engineering & Technology

College of Engineering & Technology Newsletter

June 2018 issue of Illuminator, a monthly publication of ODU's Batten College of Engineering and Technology.

Mechanism Of Virus Inactivation By Cold Atmospheric-Pressure Plasma And Plasma-Activated Water, Li Guo, Ruobing Xu, Lu Gou, Zhichao Liu, Yiming Zhao, Dingxin Liu, Lei Zhang, Hailan Chen, Michael G. Kong

Bioelectrics Publications

ABSTRACT Viruses cause serious pathogenic contamination that severely affects the environment and human health. Cold atmospheric-pressure plasma efficiently inactivates pathogenic bacteria; however, the mechanism of virus inactivation by plasma is not fully understood. In this study, surface plasma in argon mixed with 1% air and plasma-activated water was used to treat water containing bacteriophages. Both agents efficiently inactivated bacteriophages T4, ϕ174, and MS2 in a time-dependent manner. Prolonged storage had marginal effects on the antiviral activity of plasma-activated water. DNA and protein analysis revealed that the reactive species generated by plasma damaged both nucleic acids and proteins, consistent with the …

Ms Analysis Of A Dilution Series Of Bacteria: Phytoplankton To Improve Detection Of Low Abundance Bacterial Peptides, Emma Timmins-Schiffman, Molly P. Mikan, Ying Sonia Ting, H. Rodger Harvey, Brook L. Nunn

OES Faculty Publications

Assigning links between microbial activity and biogeochemical cycles in the ocean is a primary objective for ecologists and oceanographers. Bacteria represent a small ecosystem component by mass, but act as the nexus for both nutrient transformation and organic matter recycling. There are limited methods to explore the full suite of active bacterial proteins largely responsible for degradation. Mass spectrometry (MS)-based proteomics now has the potential to document bacterial physiology within these complex systems. Global proteome profiling using MS, known as data dependent acquisition (DDA), is limited by the stochastic nature of ion selection, decreasing the detection of low abundance peptides. …

Arthropod Evs Mediate Dengue Virus Transmission Through Interaction With A Tetraspanin Domain Containing Glycoprotein Tsp29fb, Ashish Vora, Wenshuo Zhou, Berlin Londono-Renteria, Michael Woodson, Michael B. Sherman, Tonya M. Collpitts, Girish Neelakanta, Hameeda Sultana

Biological Sciences Faculty Publications

Dengue virus (DENV) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. Using in vitro cell lines derived from Aedes albopictus and Aedes aegypti, the primary vectors of DENV, we report that DENV2/DENV3-infected cells secrete extracellular vesicles (EVs), including exosomes, containing infectious viral RNA and proteins. A full-length DENV2 genome, detected in arthropod EVs, was infectious to naïve mosquito and mammalian cells, including human-skin keratinocytes and blood endothelial cells. Cryo-electron microscopy showed mosquito EVs with a size range from 30 to 250 nm. Treatments with RNase A, Triton X-100, and 4G2 antibody-bead binding assays showed that infectious …

Graduate News, Graduate School, Old Dominion University

Graduate News

May 2018 issue of Graduate News from the ODU Graduate School.

College Of Health Sciences E-Newsletter, May 2018, Irvin B. Harrell (Editor)

Ellmer College of Health Sciences Newsletters

May 2018 issue of Old Dominion University's College of Health Sciences Newsletter.

Light Availability And Phytoplankton Growth Beneath Arctic Sea Ice: Integrating Observations And Modeling, Victoria J. Hill, Bonnie Light, Michael Steele, Richard C. Zimmerman

OES Faculty Publications

Observations of the seasonal light field in the upper Arctic Ocean are critical to understanding the impacts of changing Arctic ice conditions on phytoplankton growth in the water column. Here we discuss data from a new sensor system, deployed in seasonal ice cover north‐east of Utqiaġvik, Alaska in March 2014. The system was designed to provide observations of light and phytoplankton biomass in the water column during the formation of surface melt ponds and the transition from ice to open water. Hourly observations of downwelling irradiance beneath the ice (at 2.9, 6.9, and 17.9 m depths) and phytoplankton biomass (at …

Characterization And Analysis Of Ultrathin Cigs Films And Solar Cells Deposited By 3-Stage Process, Grace Rajan, Krishna Aryal, Shankar Karki, Puruswottam Aryal, Robert W. Collins, Sylvain Marsillac

Electrical & Computer Engineering Faculty Publications

In view of the large-scale utilization of Cu(In,Ga)Se₂ (CIGS) solar cells for photovoltaic application, it is of interest not only to enhance the conversion efficiency but also to reduce the thickness of the CIGS absorber layer in order to reduce the cost and improve the solar cell manufacturing throughput. In situ and real-time spectroscopic ellipsometry (RTSE) has been used conjointly with ex situ characterizations to understand the properties of ultrathin CIGS films. This enables monitoring the growth process, analyzing the optical properties of the CIGS films during deposition, and extracting composition, film thickness, grain size, and surface roughness which …

Proceedings, Msvscc 2018, Old Dominion University, Department Of Modeling, Simulation & Visualization Engineering, Old Dominion University, Virginia Modeling, Analysis & Simulation Center

Modeling, Simulation and Visualization Student Capstone Conference

Proceedings of the 12th Annual Modeling, Simulation & Visualization Student Capstone Conference held on April 19, 2018 at VMASC in Suffolk, Virginia. 155 pp.

College Of Health Sciences E-Newsletter, April 2018, Irvin B. Harrell (Editor)

Ellmer College of Health Sciences Newsletters

April 2018 issue of Old Dominion University's College of Health Sciences Newsletter.

Circulation, Vol. 23, No. 2, Center For Coastal Physical Oceanography, Old Dominion University, Michelle Covi

CCPO Circulation

Spring 2018 issue of CCPO Circulation featuring article "Connecting Science to Stakeholders for Community Resilience" by Michelle Covi.

Museum Of Unforeseen Circumstances, Ethan Forrest Ross

English Theses & Dissertations

On the day of his mother’s death, Charlie Agate abandons his current life and returns to his childhood home in Milwaukee, Wisconsin. For months, this new residency enables his thirst for isolation and comfort, but at last Charlie ventures back into the public world where he forms an unlikely bond with an elderly woman, Virginia Day. Virginia is strange and impoverished, broken and yet hopeful as she maintains an assertion so outlandish Charlie can’t help but pursue the truth of this mystery alongside her. This novella is about faith and mystery, guilt that leads to opportunity, and the persistence of …

We Are The Bobcats, Jacquelyn Mohan

English Theses & Dissertations

We are the Bobcats is a novella that follows a group of high school seniors as they undergo the loss of two of their peers. Its central themes include grief, guilt, loss, and the complications between the three in the aftermath of tragedy. The story also concerns ideas of group identity, individualism, and the disparity between external presentation and internal struggles.