Digital Commons Network^™

Validating Endosomal Antibodies For Use In Immunofluorescence Assays In A Cell-Based Model Of Prader Willi Syndrome, Hayden O'Dell

Miners Solving for Tomorrow Research Conference

Prader-Willi syndrome (PWS) is a rare progressive metabolic disease characterized by a wide range of behavioral and cognitive defects, most notably severe hyperphagia. The disorder is caused by the complete loss of some paternally inherited genes on chromosome 15q11-q13, resulting in endocrine dysfunction and a loss of control over feeding behaviors. Among the deleted genes within this region is MAGEL2. Our lab recently characterized a paternally deficient MAGEL2 rat model of PWS, discovering a defect in pituitary secretory capacity that is consistent with the impaired vesicular trafficking and secretion observed in PWS patients. To further characterize the defects in vesicular …

Ai Adoption Tensions For Organ Procurement Organizations, Joely Grace Hall

Miners Solving for Tomorrow Research Conference

Artificial intelligence (AI) has the potential to improve efficiency in healthcare, yet its adoption remains limited, with only 22% of healthcare organizations having implemented domain-specific AI tools. Adoption may be especially complex in specialized domains such as organ transplantation, where ethical, legal, and operational challenges are dominant. This study examined factors influencing AI acceptance within Organ Procurement Organizations (OPOs), focusing on technological, organizational, and environmental contexts.

Semi-structured interviews with 16 OPO executives from 10 OPOs revealed key tensions shaping AI adoption. We identified five tensions that are holding back OPO leaders from AI adoption, (1) misconceptions, (2) training approach, (3) …

Community Dynamics In Urban Ponds Could Be Influenced By Artificial Habitats, Keagan Miller, Uma Adrianna Misra

Miners Solving for Tomorrow Research Conference

Urban ponds are subject to nutrient influxes from stormwater runoff, and blooms of harmful algae can develop in response. One management strategy includes deployment of Floating Treatment Wetlands (FTWs) or floating mats of aquatic plants designed to maximize uptake of nutrients to plant biomass. An understudied component of these artificial systems is their potential to provide microhabitat for aquatic biota. This study focused on the intersection of aquatic insect communities and fish presence between natural and artificial habitat types. Insect communities were found to be weakly dissimilar between habitat types (ANOSIM: R=0.254, p=0.048). Specifically, Anisoptera and Zygoptera were absent or …

2nd Annual Miners Solving For Tomorrow Research Conference - Undergraduate Schedule, Blake Bowman, Carter Lawson, Celia Freed, Adam Merchiori, Allie Dingfield, Catherine Skaggs, Drake O'Leary, Quinten Bachman, Elizabeth Bone, Joely Grace Hall, William Wisnasky, James Meyer, Sindhujaa Jaiganesh, Sophia Nicolette Petilla Militante, Brantley Carter, Adrianna Sasser, Alec Gamache, Micah Gargrave, Keagen Miller, Uma Adrianna Misra, Collin Gentry, Christy Johnson, Brady Carlson, Logan Williamson, Jivanji Sumariwalla, Hayden O'Dell, Elijah Brakensiek, Katharine Gray, Lukas Farthing, Jacob Harl, Landon Meyer, Nehemiah Milton, Lana Herkenhoff, Jenna Mueller, Lucas Ethington, Maya Southard, Aster Davidson, Ethan Beane, Jacob Penn, Connor Jordan, Amanda Hodges, Ethan Keuhn, Milan Jebaraj, Ariel Pilger, Sydney Clark, Maris Reinkemeyer, Manav Raja Vinotha, Punit Sesha Sai Turlapati, Benjamin Sullins, Benjamin Biehl, Jamie Koester, Aidan Sengupta

Miners Solving for Tomorrow Research Conference

No abstract provided.

2nd Annual Miners Solving For Tomorrow Research Conference, Blake Bowman, Carter Lawson, Celia Freed, Adam Merchiori, Allie Dingfield, Catherine Skaggs, Drake O'Leary, Quinten Bachman, Elizabeth Bone, Joely Grace Hall, William Wisnasky, James Meyer, Sindhujaa Jaiganesh, Sophia Nicolette Petilla Militante, Brantley Carter, Adrianna Sasser, Alec Gamache, Keagan Miller, Uma Adrianna Misra, Collin Gentry, Christy Johnson, Brady Carlson, Logan Williamson, Jivanji Sumariwalla, Hayden O'Dell, Elijah Brakensiek, Katharine Gray, Lukas Farthing, Jacob Harl, Landon Meyer, Nehemiah Milton, Lana Herkenhoff, Jenna Mueller, Lucas Ethington, Maya Southard, Aster Davidson, Ethan Beane, Jacob Penn, Connor Jordan, Amanda Hodges, Ethan Keuhn, Milan Jebaraj, Ariel Pilger, Sydney Clark, Maris Reinkemeyer, Manav Raja Vinotha, Punit Sesha Sai Turlapati, Benjamin Sullins, Benjamin Biehl, Jamie Koester, Aidan Sengupta, Antai Dong, Connor Bell, Dennis Dadzie, Joshua Gary, Adebayo Olayinka Oke, Deeshani Mitra, Yejun Kim, Josiah Mcdermott, Samiksha Aryal, Nicholas Brenner, Timothy Ennis, Maryam Sharifi Paroushi, Sujan Maharjan, Md Saiduzzaman, Hari Dhital, Manuela Isabel Arenas Alvarez, Eyuel A Getahun, Lucas Greiner, Amaneh Babaee, Shane Cairns, Nazish Khalid, Joseph Walton, Jie Shi, Ehsan Asheghianamiri, Mariam Elazhary, Makuach James Makeny Panther Athach, Rasman Mubtasaim Swargo, Shiva Kumar Goud Kasani, Pablo Jara, Sara Mccauley, Hartzell Weston, Al Mojahid Afridi, Matik Heskin, Nima Mahmoudzadeh, Effat Eskandari, Amitav Sen, Rabin Pandey, Amirhossein Habibi, Nuzaer Omar, Mizanur Rahman Jewel, Nathan Tibbetts, Manoj Twarakavi, Fahara Bristy, Akhrorbek Narmatov, Mahnaz Asgari Sooran, Tafang Hei, Nathan Roberts, Khosro Ghorbani Zadeh

Miners Solving for Tomorrow Research Conference

No abstract provided.

April 2026 Cafe Newsletter, Missouri University Of Science And Technology

CAFE Faculty Newsletters

Center for Advancing Faculty Excellence (CAFE) newsletter April 2026

The Kaczmarz Algorithm In Hilbert C∗-Modules, Daniel Alpay, Chad Berner, Eric S. Weber

Mathematics and Statistics Faculty Research & Creative Works

The Kaczmarz algorithm in Hilbert spaces is a classical iterative method for stably recovering vectors from inner product data. In this paper, we extend the algorithm to the setting of Hilbert C^∗-modules and establish analogues of its effectiveness in both finite-dimensional and stationary cases. Consequently, we demonstrate that continuous families of elements in a Hilbert space can be uniformly recovered using the Kaczmarz algorithm. Additionally, we develop a normalized Cauchy transform for continuous families of measures and use it to provide sufficient conditions under which standard frames in Hilbert C(X)-modules can be generated by the Kaczmarz algorithm and …

A Fully Discrete Decoupled Scheme And Applications For Non-Isothermal Two-Phase Flow Model With Different Viscosities And Thermal Diffusivities, Jian Li, Chunhao Chen, Xiaoyong Chen, Rui Li, Xiaoming He

Mathematics and Statistics Faculty Research & Creative Works

To study natural convection problems in two-phase flows, a non-isothermal two-phase flow model incorporating differential viscosities and thermal diffusivities is considered and analyzed via the phase-field method. This modeling framework involves the Multiphysics coupling of the Cahn-Hilliard phase field equations, heat transfer equation, and Navier-Stokes equations, resulting in a strongly nonlinear system. To efficiently solve the sophisticated system, we develop, analyze, and demonstrate a decoupled linear fully discrete scheme, which leverages the invariant energy quadratization strategy for the Cahn-Hilliard phase field system, the artificial compressibility method without artificial pressure boundary condition, an explicit-implicit treatment of nonlinear terms, and the addition …

Sequences That Do Frame Reconstruction, Chad Berner

Mathematics and Statistics Faculty Research & Creative Works

Frames allow all elements of a Hilbert space to be reconstructed by inner product data in a stable manner. Recently, there is interest in relaxing the definition of frames to understand the implications for stable signal recovery. In this paper, we relax the definition of a frame by allowing the operator in the frame decomposition formula to not be invertible. We provide a complete classification of sequences that allow this decomposition via a type of frame operator. In addition, we provide several examples of sequences that allow this reconstruction property that are not frames and illustrate in which ways they …

Correlated Many-Body Quantum Dynamics Of The Peregrine Soliton, D. Diplaris, G. A. Bougas, P. G. Kevrekidis, C. L. Hung, P. Schmelcher, S. I. Mistakidis

Physics Faculty Research & Creative Works

We explore the correlated dynamics underlying the formation of the quantum Peregrine soliton, a prototypical rogue-wave excitation, utilizing interaction quenches from repulsive to attractive couplings in an ultracold bosonic gas confined in a one-dimensional box trap. The latter emulates the so-called semiclassical initial conditions and the associated gradient catastrophe scenario facilitating the emergence of a high-density, doubly localized waveform. The ensuing multiorbital variant of the Peregrine soliton features notable deviations from its mean-field sibling, including a reduced peak amplitude, wider core, absence of the side density dips, and earlier formation times. Moreover, Peregrine soliton generation yields coherence losses, while experiencing …

Additive Manufacturing Of Ti-Ni Based Ternary Shape Memory Alloys, Yitao Chen, Frank Liou

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Metal additive manufacturing has become a powerful tool to develop customized metal alloys and to discover more advanced properties for novel extended applications. Ti-Ni based shape memory alloy is a group of intriguing smart functional materials, and adding a small amount of a third element can promote and induce more attractive functions. Due to the difficulty in traditional processing and the unique feature of material flexibility of in-situ alloying in additive manufacturing processes, not only Ti-Ni binary shape memory alloys but also Ti-Ni-X ternary shape memory alloys can be developed, manufactured, and investigated in-depth by additive manufacturing. This paper provides …

An Ultrafine-Resolution Numerical Investigation Of The Influence Of Terrain On Tornado Behavior, Jiamin Dang, Jana Houser, Leigh Orf, Peng Yue, Guirong Yan

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

This study investigates the effects of idealized and realistic terrain on tornado characteristics and behavior. It uses a novel simulation approach, nesting a high-fidelity, ultrafine-resolution, tornado-scale, engineering large-eddy simulation (LES) within a Cloud Model 1 (CM1) simulation of a tornadic supercell. We analyze the effects of terrain on the tornado's central pressure, horizontal and vertical velocities, vortex shape, and path. Seven idealized terrain configurations are used including 1) a control run with flat ground, 2) and 3) an idealized hill with steep and gradual slopes having the height of 25.4 m, 4) and 5) an idealized escarpment with steep and …

Analysis Of Live Load Distribution Factors In Prestressed Concrete Girder-Bridges With Asymmetric Losses In Prestressing Strands, Mohamed T. Elshazli, Mohamed Elgawady, Ahmed Ibrahim

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Asymmetric loss of prestressing strands in concrete bridge girders presents a critical yet underrecognized threat to structural integrity, particularly in exterior girders subjected to impact or localized deterioration. This form of localized and unbalanced damage leads to eccentric reductions in prestressing force, rotation of the principal axis, and diminished flexural capacity, effects not explicitly addressed in current design standards such as the AASHTO LRFD Bridge Design Specifications. This study presents a comprehensive numerical investigation into the structural implications of such asymmetric damage, focusing on its effect on live load distribution factors (LLDFs) across prestressed girder bridges. A parametric study of …

Near-Wall Void Distribution Characterization In Pebble Bed Reactor Using Gamma-Ray Ct And Dem Simulation, Ahmed Jasim, Mauricio Maestri, Abdullah Al Zubaidi, Omar Farid, Muthanna Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Accurate characterization of void-fraction distributions in pebble-bed reactors (PBRs) is essential for predicting flow, heat transfer, and neutronic behavior. High-fidelity experimental benchmark data for validating such predictions remain scarce, largely due to the challenges of non-invasive measurements. In this study, gamma-ray computed tomography (CT) was employed to measure radial and cross-sectional porosity in a laboratory-scale pebble bed containing 6cm graphite pebbles. A Discrete Element Method (DEM) simulation was implemented and validated against these measurements, then applied to the full-scale geometry of the Xe-100 high-temperature gas-cooled pebble-bed reactor. Analyses included radial and axial void-fraction profiles in the cylindrical section and conical …

Graphene Synthesis: A Reactor-Oriented Review Of Conventional And Emerging Production Methods, Paul C. Ani, Zeyad Zeitoun, Hasan J. Al-Abedi, Joseph D. Smith

Chemical and Biochemical Engineering Faculty Research & Creative Works

Graphene's unparalleled electrical, mechanical, and thermal properties have positioned it as a transformative material across diverse sectors, including electronics, energy storage, biomedicine, and environmental remediation. However, scalable, cost-effective, and high-quality production remains a critical challenge. This review presents a comprehensive, reactor-oriented analysis of both conventional and emerging graphene synthesis methods, categorized into top-down and bottom-up approaches. Special emphasis is placed on the role of reactor configurations in determining product quality, layer control, scalability, and economic viability. Key synthesis techniques explored include chemical vapor deposition (CVD), epitaxial growth, electrochemical exfoliation, ultrasonic-assisted methods, microwave reactors, combustion synthesis, and plasma-enhanced processes. By evaluating …

A Dataset Of Precipitate-Containing Multi-Principal Element Alloys, Anshu Raj, Xin Wang, Matthew Luebbe, Haiming Wen, Kun Lu, Shuozhi Xu

Materials Science and Engineering Faculty Research & Creative Works

We report a curated dataset that brings together composition, processing conditions, microstructural details, and mechanical properties for 396 combinations of alloy composition and processing condition drawn from 100 peer-reviewed research articles on precipitate-containing multi-principal element alloys (MPEAs). The dataset was created by first utilizing a generative large language model for information extraction, followed by expert review to ensure accurate recovery of materials data. Compositional information was taken directly from tables and text, while processing routes — including homogenization, rolling, recrystallization, and aging — were converted into uniform temperature and time metrics. Microstructural descriptors, including precipitate phases and sizes, were consolidated …

Ac Magnetic Measurements With A Self-Oscillating Lc Circuit And Its Application To University Education, Harshit Agarwal, Oleksandra Uralska, Jasmin Billingsley, Maxim Yamilov, Hyunsoo Kim

Physics Faculty Research & Creative Works

Editor's Note: This paper is a welcome tutorial on a technique for AC magnetometry, allowing the measurement of a sample's magnetic susceptibility with considerable sensitivity. The authors present a low-cost setup based on an LC self-oscillator circuit, which has an operating frequency that is rather sensitive to the susceptibility of a sample introduced within its inductor. After characterizing the performance of the circuit, data taken on a high-temperature superconducting sample are presented, demonstrating how the samples superconducting transition can be detected by this noncontact magnetic method, independent of the usual resistivity-based detection. This project will be of interest to instructors …

Corrigendum To “Advanced Techniques In Quartz Wafer Precision Processing: Stealth Dicing Based On Filament-Induced Laser Machining” [Opt. Laser Technol. 171 (2024) 110474] (Optics And Laser Technology (2024) 171, (S0030399223013671), (10.1016/J.Optlastec.2023.110474)), Yun Wang, Yutang Dai, Farhan Mumtaz, Kaiyan Luo

Electrical and Computer Engineering Faculty Research & Creative Works

The authors regret, that the affiliation for author Yun Wang was incomplete. To accurately reflect both the author's academic affiliation and the research platform where the work was conducted. The correct affiliation for Yun Wang is updated as above. The authors would like to apologize for any inconvenience caused.

Visual Pattern Mining With Similarity Metrics For Model-Free Trading In The Korean Futures Market, Juhyeon Jang, Jaeyun Kim, David Enke

Engineering Management and Systems Engineering Faculty Research & Creative Works

The fractal market hypothesis highlights multi-scale dynamics in financial time series and provides a theoretical foundation for pattern-based analysis. This study proposes a model-free visual pattern mining framework that transforms high-frequency market data into image representations to support intelligent decision-making. By converting 1-minute KOSPI200 futures data into candlestick chart and Bollinger band images, the method effectively captures structural patterns and volatility dynamics. The framework applies similarity metrics and Intersection over Union (IoU)-based visual comparison to identify historically similar patterns and generate intelligent trading signals without model training or complex parameter tuning. Experimental results demonstrate that combining visual features of candlestick …

Dendrimer Nanogels With Built-In Free Radical Scavenging Enable Efficient Topical Delivery Of A Hydrophilic Antioxidant To Restore Lens Redox Balance For Cataract Treatment, Lin Qi, Huari Kou, Anna Chernatynskaya, Da Huang, Vimalin Jeyalatha Mani, Humeyra Karacal, Nuran Ercal, Hu Yang

Chemistry Faculty Research & Creative Works

Cataract is a leading cause of vision impairment worldwide and are primarily caused by oxidative stress that damages and aggregates lens proteins, leading to lens opacification. However, the eye's anatomical barriers limit the penetration and bioavailability of antioxidant therapies. To address this challenge, a dendrimer-based nanogel with a built-in reactive oxygen species (ROS)-scavenging capability developed by us was employed to deliver the antioxidant N-acetylcysteine (NAC) to the lens. NAC was loaded into a generation-5 PEGylated poly(amidoamine) dendrimer (G5-PEG-TK, termed the GPT) nanogel. The resulting NAC-GPT was characterized for its ROS-scavenging activity, bioavailability, and corneal permeability. The efficacy of NAC-GPT was …

Efficiency Comparison Of Reduced Graphene Oxide (Rgo) Composite-Incorporated Polyacrylonitrile Nanofibrous Filters For Respirable Crystalline Silica Dust And Coal Dust, Mahsa Moradi, Ashish Kakoria, Lina Zheng, Guang Xu

Mining Engineering Faculty Research & Creative Works

The health of miners working in underground mines is greatly threatened by their exposure to respirable crystalline silica (RCS) found in coal dust. While coal dust includes RCS, it is important to know the specific filtration efficiency for RCS particles compared to coal dust to improve protective measures. This study compares the filtration efficiency of reduced graphene oxide (rGO) composite-incorporated Polyacrylonitrile (PAN) nanofibrous filters at four different concentrations of rGO (0, 2, 6, and 10 wt.%) for RCS particles and coal dust. The filters were made using the electrospinning method. They were tested separately with RCS particles and coal dust …

Propagation And Interaction Of Gravity Currents Induced By Successive Release Of Two Lock Fluids, Khem Prasad Bhattarai, Subhas Karan Venayagamoorthy

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

This study investigates the dynamics of sequentially released gravity currents in a lock-exchange configuration consisting of two lock fluids using high-resolution numerical simulations and compares them with the classical single-lock exchange. The results demonstrate that, in a two-lock-fluid configuration, the overtaking of the lighter lock current by the heavier lock current alters the current's front dynamics, leading to complex velocity transitions. Before overtaking occurs, the front propagation is slower than in the classical single-lock-fluid case because of lower density contrast, but after overtaking, the heavier lock fluid accumulates at the head region of the current and enhances its speed. The …

Voltage Breakdown Strength And Energy Density Of Ceramic Srtio₃ At Cryogenic Temperatures From 295 K To 77 K, Hung Trinh, Alan Devoe, Fatih Dogan

Materials Science and Engineering Faculty Research & Creative Works

The low-temperature dependence of the DC dielectric breakdown strength (EBD) and the energy storage density (W) of capacitors, fabricated from high purity polycrystalline strontium titanate, were investigated in the temperature range from 295 K to 77 K. Test samples were constructed with a process similar to that used for multilayer ceramic capacitors (MLCCs). The permittivity, loss tangent, leakage current, and breakdown strength were measured as a function of temperature and voltage. Weibull statistical analysis was used to quantify breakdown distributions. Despite electric-field suppression of permittivity, breakdown strength increased significantly with decreasing temperatures, leading to enhanced energy density. The breakdown field …

Flaked Paraffin Wax-Based Thermally Enhanced Composite Phase Change Materials: Toward A Highly Efficient Thermally Responsive Temperature-Regulating Asphalt Pavement, Feng Ma, Meng Pang, Zhen Fu, Xinye Jiang, Qiang An, Yingjie Hou, Wenhao Dong, Jenny Liu, Jiasheng Dai

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

To mitigate high-temperature distress in asphalt pavements and enhance thermal regulation, flaked paraffin wax (FPW) was selected as the PCM and incorporated into asphalt. To prevent leakage, FPW was microencapsulated (FPW-MPCM). To boost thermal conductivity, FPW-MPCM was combined with expanded graphite (EG) via two methods: One method involves using EG as an adsorption carrier to prepare EG-adsorbed FPW phase change microcapsules (FPW-MPCM-EG); the other method uses EG as the core material to prepare FPW/EG graphite phase change microcapsules (FPW/EG-MPCM). These four PCMs were added to asphalt at 3 %, 6 %, 9 %, and 12 % (wt.%) to prepare phase-change …

Precision Spectroscopy Of 2s-Ns Transitions In Atomic Hydrogen: A Determination Of The Proton Charge Radius, R. G. Bullis, W. L. Tavis, M. R. Weiss, Orellana O. Cisneros, A. J. Cheeseman, Ulrich D. Jentschura, D. C. Yost

Physics Faculty Research & Creative Works

We present absolute frequency measurements of 2S_1/2-nS_1/2 two-photon transitions with n=8, 9, and 10 in a cryogenic beam of atomic hydrogen. Each transition has been measured with a fractional uncertainty of ≈2.6x10^-12. Combining the results from this Letter and the 1S_1/2-2S_1/2 transition frequency, we extract a root-mean-square proton radius of rp=0.8433(31) fm and a Rydberg frequency of cR_∞=3 289 841 960 252.9(9.7) kHz. These are in good agreement with the CODATA 2022 recommended values.

Wasting The Risk, Or Risking The Waste? Understanding The Trends Of Critical Raw Material Loss Into Waste Streams During Copper And Aluminium Processing, Ella Lausberg, Joël Brugger, Rahul Ram, John R. Owen, Deanna Kemp, Micheal S. Moats, Jonathan Hamisi, Vanessa N.L. Wong

Materials Science and Engineering Faculty Research & Creative Works

Many critical raw materials (CRM) necessary in the transition to carbon-neutral energy reside in the waste streams of mining projects, as they are by-products and often not recovered alongside the primary metal. This work aims to document (i) the loss and potential recovery of by-product metals during the host commodity processing and (ii) the consequences of non-recovery, via a multi-scale risk–reward analysis. The information on the deportment of by-product metals through processing circuits is crucial for treating them as a resource, without which they risk near-permanent loss when treated as 'waste'. We review the deportment of tellurium and selenium as …

Ionic Liquid Pilocarpine Serves As Therapeutic Cosolvent And Permeation Enhancer For Glaucoma Medication, Ashish Trital, Lei Xu, Burhan Ates, Tzu Chen Wang, Vimalin Mani, Hu Yang

Chemical and Biochemical Engineering Faculty Research & Creative Works

The efficacy of hydrophobic ocular drugs is significantly hindered by their low bioavailability, for which poor water solubility is a major contributing factor. In this work, we synthesized pilocarpine-derived ionic liquid [Pilo-OEG] Cl (PO) and evaluated its cosolvent properties for the codelivery of the hydrophobic antiglaucoma drug brimonidine (BM) for glaucoma therapy. Pilocarpine was quaternized with 2-[2-(2-chloroethoxy) ethoxy] ethanol in a one-pot reaction to yield PO, and its structure was confirmed using ¹H NMR and FT-IR spectroscopy methods and further characterized for its rheological property and thermal stability. The HET-CAM assay and cell viability study showed that PO was …

Guiding Fast Ion Beam By Suppressing Secondary Ions, Yingli Xue, Junliang Liu, Mingwu Zhang, Daniel Fischer, Guoxing Xia, Nikolaus Stolterfoht, Reinhold Schuch, Yehong Wu, Bian Yang, Xiaoxiao Li, Caojie Shao, Wei Wang, Zhangyong Song, Xing Fang

Physics Faculty Research & Creative Works

We demonstrate that secondary ions sputtered from a macrocapillary's inner surface by the primary beam induce premature saturation of the guiding field, hindering fast ion guiding. By suppressing secondary ion sputtering with grooved surfaces, we achieve a guiding-field potential difference exceeding 1 kV, 2 orders of magnitude larger than previously achieved in stable ion guiding. This enables stable guiding of a 20-keV/q O5+ beam at offset angles up to 15°, with transmitted ions retaining their initial energy and charge state. A self-consistent field model accurately reproduces these results, revealing that guiding performance relies on managing secondary ions and the channel's …

Fluorescent Biosensor-Guided Engineering Of Enzyme Cascades For Electrochemical Applications, Nathan J. Ricks, Monica Brachi, Fnu Arun, Egor Baiarashov, Shelley D. Minteer, Ming C. Hammond

Chemistry Faculty Research & Creative Works

Substrate channeling is a strategy for enhancing flux and yield in enzymatic cascades and is increasingly relevant for applications in biocatalysis, biotechnology, and bioelectrochemical systems. However, efforts to engineer channeling are limited by the lack of high-throughput methods to evaluate and optimize channeling efficiency. Here, we present a fluorescence-based screening assay to rapidly assess substrate channeling in a model system involving fumarase and malate dehydrogenase, two sequential enzymes from the Krebs cycle. By expressing genetic fusions in Escherichia coli, quantifying intermediate (malate) and product (NADH) formation in lysate using orthogonal fluorescent readouts, and comparing product-to-intermediate ratios, we screened a library …

An Adaptive Immersive Training Framework For Miner Self-Escape Readiness In Underground Mining Emergencies, Muhammad Azeem Raza, Samuel Frimpong, Saima Ghazal

Mining Engineering Faculty Research & Creative Works

Underground mining environments are complex and hazardous operations where emergencies continue to happen. Underground mine emergencies require rapid, high-stakes decision-making under conditions of uncertainty, stress, and limited visibility. Conventional mine emergency training largely relies on instruction-based approaches which provide insufficient exposure to the cognitive and behavioral demands of real underground emergency situations. There has been an identified need to train miners for knowledge, skills, abilities, and other characteristics (KSAOs). This study proposes an Adaptive Immersive Training Framework (AITF), a cognitively grounded architecture that integrates cognitive task analysis (CTA), KSAOs, and situational awareness assessment for miner self-escape training and readiness. The …