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Money And Tragedy In The Nineteenth-Century Novel, Clany Soileau Jan 2006

Money And Tragedy In The Nineteenth-Century Novel, Clany Soileau

LSU Doctoral Dissertations

The nineteenth-century novelists studied in this dissertation used tragic form to investigate economic and social changes taking place around them. Honoré de Balzac’s Le Père Goriot (1834), William Dean Howells’ The Rise of Silas Lapham (1884-1885), Giovanni Verga’s Mastro-don Gesualdo (1888), Benito Pérez Galdós’s Miau, (1888), and Thomas Mann’s Buddenbrooks (1901) reflect the interest of writers in France, the United States, Italy, Spain, and Germany in questions concerning how money in an evolving capitalist society not only had a major role in shaping the behavior and personalities of specific individuals but also affected such institutions as the family. Under these …

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Numerical Techniques For Fresnel Diffraction In Computational Holography, Richard Patrick Muffoletto Jan 2006

Numerical Techniques For Fresnel Diffraction In Computational Holography, Richard Patrick Muffoletto

LSU Doctoral Dissertations

Optical holography can produce very realistic virtual images due to its capability to properly convey the depth cues that we use to interpret three-dimensional objects. Computational holography is the use of digital representations plus computational methods to carry out the holographic operations of construction and reconstruction. The large computational requirements of holographic simulations prohibit present-day existence of real-time holographic displays comparable in size to traditional two-dimensional displays. Fourier-based approaches to calculate the Fresnel diffraction of light provide one of the most efficient algorithms for holographic computations because this permits the use of the fast Fourier transform (FFT). The limitations on …

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Effects Of Diesel-Fuel And Copper Contaminants On Benthic Microalgae, Soraya Silva Jan 2006

Effects Of Diesel-Fuel And Copper Contaminants On Benthic Microalgae, Soraya Silva

LSU Doctoral Dissertations

Salt marshes are dynamic, highly productive habitats and serve as nursery grounds for many commercially and economically important species. Benthic microalgae (BMA) are considered an important food source for benthos and provide the principal source of nutrition that fuels secondary production. Estuarine sediments around the world are a repository for many contaminants from anthropogenic sources. In particular, hydrocarbons and metals are ubiquitous contaminants in coastal systems. The primary goal of this study was to study the effects of diesel fuel and copper, alone and in combination, on the BMA assemblage from a coastal salt marsh. To achieve this objective, salt …

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Factors Influencing The Participation In Environmental Stewardship Programs: A Case Study Of The Agricultural And Forestry Sectors In Louisiana, Carrie Castille Mendoza Jan 2006

Factors Influencing The Participation In Environmental Stewardship Programs: A Case Study Of The Agricultural And Forestry Sectors In Louisiana, Carrie Castille Mendoza

LSU Doctoral Dissertations

Considerable research has been conducted to evaluate the adoption of agricultural best management practices and their overall impact on improving environmental quality. However, limited studies have been conducted to evaluate the behavioral factors that influence the adoption of these practices in the context of educational programs The goal of this study is to determine the factors that influence farmer conservation behavior that might lead to an increased probability of improving agriculture and forestry watersheds. A conceptual model was developed to identify the: 1) identify landowner participation in watershed conservation projects, and 2) determine the factors influencing agriculture and forestry landowners …

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Phylogeny Of Hinterhubera, Novenia And Related Genera Based On The Nuclear Ribosomal (Nr) Dna Sequence Data (Asteraceae: Astereae), Vesna Karaman Jan 2006

Phylogeny Of Hinterhubera, Novenia And Related Genera Based On The Nuclear Ribosomal (Nr) Dna Sequence Data (Asteraceae: Astereae), Vesna Karaman

LSU Doctoral Dissertations

Analyses of ITS, ETS and matK sequences for genera of the subtribe Hinterhuberinae, including representatives of 17 subtribes from the tribe Astereae, rooted on Anthemideae, Calenduleae and Inuleae, provide no support for the monophyly of the Hinterhuberinae. Chiliotrichum and Celmisia groups, Olearia in part, Novenia, Oritrophium and Pteronia are in the basal group that also includes subtribe Felicinae. Madagaster, Remya, Olearia in part, Achnophora and Hinterhubera group lie in the Southern Hemisphere grade, with Asterinae, Bellidinae, Brachycominae, Grangeinae, Podocominae, Lagenophorinae and Baccharidinae. Monophyly of Asterinae, Grangeinae, Podocominae and Lagenophorinae is not supported. Instead South American representatives of Lagenophorinae, Grangeinae and …

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The Epidemiology Of West Nile Virus In Louisiana, Karen Ruth Gruszynski Jan 2006

The Epidemiology Of West Nile Virus In Louisiana, Karen Ruth Gruszynski

LSU Doctoral Dissertations

West Nile virus (WNV), a member of the genus Flavivirus transmitted by mosquitoes, first appeared in the New York in 1999. Within five years WNV was detected throughout the contiguous 48 states causing disease in reservoirs and accidental hosts alike. In Louisiana, WNV was first detected in 2001 with one human case, ten equine cases, and six dead birds reported. The introduction of WNV into Louisiana presented an unique opportunity to observe an emerging disease unfold, so a study was launched to gain insight into the epidemiology of WNV in Louisiana. The first component, an environmental predictive model for West …

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A Comparative Study Of Household Demand For Meats By U.S. Hispanics, Carlos Ignacio Garcia Jan 2006

A Comparative Study Of Household Demand For Meats By U.S. Hispanics, Carlos Ignacio Garcia

LSU Master's Theses

The general objective of this research project was to study the consumption behavior of U.S. Hispanics for meat products. A comprehensive review of literature on the studies of U.S. Hispanic consumers was performed by means of a systematic survey; different sources of information useful in studying Hispanic consumers were documented. The simultaneous increase in population and income of Hispanics in the United States is attractive to food companies, and makes more relevant the study of their consumer behavior patterns for discovering future market opportunities. Censored incomplete demand systems of the LinQuad form were used for recognizing the consumption patterns by …

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A Thermodynamic Model For Wear In Sliding Contact, Susheel Brahmeshwarkar Jan 2006

A Thermodynamic Model For Wear In Sliding Contact, Susheel Brahmeshwarkar

LSU Master's Theses

Consideration of wear, an irreversible phenomenon, is a very important criterion in design. The knowledge of wear and its behavior enables one to make major considerations to conceptualize and design efficient machinery components with enhanced performance and reliability. The present work deals with the introduction of a novel approach of correlating wear with the thermodynamic properties of the system. The approach involves relating wear to thermodynamic entropy flow in the system using the laws of thermodynamics. This relation is verified experimentally and theoretically by considering a sliding contact in a disk-on-disk configuration for two sets of contacting materials namely Bronze …

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Green Hydrogen: Site Selection Analysis For Potential Biomass Hydrogen Production Facility In The Texas-Louisiana Coastal Region, Bryan Michael Landry Jan 2006

Green Hydrogen: Site Selection Analysis For Potential Biomass Hydrogen Production Facility In The Texas-Louisiana Coastal Region, Bryan Michael Landry

LSU Master's Theses

Hydrogen and the “Hydrogen Economy” are increasingly becoming buzzwords in discussions regarding future U.S. energy scenarios. Hydrogen energy offers a multitude of economic and environmental advantages over the current world energy structure. Despite this attention, there have been very few geographical studies of a possible transition to a hydrogen system. Even these studies have been limited in scope to demand-side analyses. This thesis attempts to rectify this situation by broadening the scope of geographical studies of hydrogen through the analysis of supply-side scenario. This study is a site selection model for a biomass hydrogen facility in the Gulf Coast of …

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The Impact Of Hypoxia On Mercury Methylation In Bottom Sediment Of Northern Gulf Of Mexico, Mei Huey Tan Jan 2006

The Impact Of Hypoxia On Mercury Methylation In Bottom Sediment Of Northern Gulf Of Mexico, Mei Huey Tan

LSU Master's Theses

Widespread concern has developed about high mercury content in fish in the Gulf of Mexico and adjacent estuaries and bays. Among the areas implicated as possible sources of the mercury that moves up the food chain from the methylmercury formed in sediments and anoxic waters is the seasonal hypoxic zone in the northern Gulf. This research was designed to determine if methylmercury formation is stimulated by the anaerobic sediment conditions accompanying the onset of summer hypoxia in the Gulf. Both field and laboratory studies were carried out. For the field study sediment samples were collected at three stations (i.e. C4, …

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Evaluating The Spatial Ecology Of Anthrax In North America: Examining Epidemiological Components Across Multiple Geographic Scales Using A Gis-Based Approach, Jason Kenna Blackburn Jan 2006

Evaluating The Spatial Ecology Of Anthrax In North America: Examining Epidemiological Components Across Multiple Geographic Scales Using A Gis-Based Approach, Jason Kenna Blackburn

LSU Doctoral Dissertations

This dissertation explores the spatial ecology and potential pathways of infection of anthrax, Bacillus anthracis, in North America. A multi-scale approach was used to evaluate the components required for disease agent survival in the environment, interactions with wildlife, and the potential role that vectors play in anthrax transmission. First, ecological niche modeling with the Genetic Algorithm for Rule-set Production (GARP) was used to predict the geographic distribution of anthrax in the continental U.S. using case data from outbreaks between 1957 and 2005. These results were then used to produce the first quantitative, continental scale predictions of anthrax in Mexico. At …

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Links Between Environmental Mercury, Special Education, And Autism In Louisiana, Jessica Rury Jan 2006

Links Between Environmental Mercury, Special Education, And Autism In Louisiana, Jessica Rury

LSU Master's Theses

The number of children born every year with neurological disorders is increasing and some of this increase may be attributed to mercury exposure. Pregnant women ingest contaminated fish, which transfers high mercury concentrations to the unborn fetus. This exposure may result in methyl mercury buildup in the brain of the unborn fetus. Symptoms ranging from minor to severe may be observed as special educational needs in school systems. These include learning disorders, developmental delays, and autism. Louisianans are especially susceptible to mercury contamination because fish and shellfish are a major portion of their cultural diet. This study, through the use …

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Coastal Marsh Restoration Using Terraces: Effects On Waterbird Habitat In Louisiana's Chenier Plain, Jessica L. O'Connell Jan 2006

Coastal Marsh Restoration Using Terraces: Effects On Waterbird Habitat In Louisiana's Chenier Plain, Jessica L. O'Connell

LSU Master's Theses

Terracing is a novel technique used to combat coastal marsh loss in Louisiana and Texas. Terraces are assumed to slow marsh erosion, decrease pond depth, and encourage vegetation expansion. Terraced ponds have never been evaluated as habitat for waterbirds, which heavily depend on Louisiana�s coastal marshes. From April 2005 to April 2006, I monitored waterbird species richness and density through time to estimate effects that terracing has on habitat quality. Water quality (turbidity, salinity, conductivity, water temperature, and water depth) also was measured. Submerged aquatic vegetation (SAV) biomass and nekton density were measured from April 2005 to September 2005. I …

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The Meaning Of Masculinity For Male Baccalaureate Nursing Program Graduates, Kenneth Tillman Jan 2006

The Meaning Of Masculinity For Male Baccalaureate Nursing Program Graduates, Kenneth Tillman

LSU Doctoral Dissertations

Previous researchers have reported that societal definitions of masculinity may act as a barrier to men entering the nursing profession, and that men who do enter nursing may hold a different view of masculinity. The purpose of this study was to describe the meaning of masculinity for recent male baccalaureate nursing program graduates. In addition, the study explored how the participants’ perceived meaning of masculinity shaped or influenced their nursing education experiences, and in turn how their nursing education experiences may have shaped or influenced their perceived meaning of masculinity. A phenomenological research design was used to study eight male …

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Liminality In Gender, Race, And Nation In Les Quarteronnes De La Nouvelle-Orléans By Sidonie De La Houssaye, Christine Koch Harris Jan 2006

Liminality In Gender, Race, And Nation In Les Quarteronnes De La Nouvelle-Orléans By Sidonie De La Houssaye, Christine Koch Harris

LSU Doctoral Dissertations

This project examines themes of race, gender, and nation in a series of four novels by nineteenth-century Louisiana author Sidonie de la Houssaye. The series, called Les Quarteronnes de la Nouvelle-Orléans (The Quadroons of New Orleans), is based on the system of plaçage. Plaçage, a system of concubinage in which white men took women of mixed racial heritage (such as “quadroons”) as mistresses, becomes a source of conflict and contradiction in the series. The author sees plaçage as a tragic necessity for some educated and morally “upright” quarteronnes. For others, those quarteronnes depicted as libidinous and avaricious, it is a …

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Belowground Biomass Of Spartina Alterniflora: Seasonal Variability And Response To Nutrients, Faith Armand Darby Jan 2006

Belowground Biomass Of Spartina Alterniflora: Seasonal Variability And Response To Nutrients, Faith Armand Darby

LSU Doctoral Dissertations

Spartina alterniflora is a salt marsh macrophyte found from Canada to the Gulf of Mexico which often provides the dominant plant cover. Although S. alterniflora is well known for its high aboveground productivity, fifty to ninety percent of the total plant production occurs belowground. No previous studies address the seasonal variation of belowground biomass or the response of above-and belowground biomass to nutrients at the southern limits of its U. S. range. The objectives of this study were to: 1) document the seasonal variability of its above- and belowground biomass and test for responses to various combinations of N, P, …

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Evolutionary Patterns In Snake Mitochondrial Genomes, Zhijie Jiang Jan 2006

Evolutionary Patterns In Snake Mitochondrial Genomes, Zhijie Jiang

LSU Doctoral Dissertations

In this dissertation I describe a number of patterns and interesting aspects associated with the evolution of snake mitochondrial genomes (mtDNA). I also attempt to resolve the phylogeny of squamates, focusing on the relationship between the snakes and lizards. The results of this study indicate that snakes and worm lizards (amphisbaenians) appear to share an exclusive common ancestor, and snakes appear to have undergone strong selective pressure that shaped snake mtDNAs. Snake mtDNAs have several unique features, including a compact size, duplicated control regions, and an elevated evolutionary rate. Based on the correlation resulting from the asymmetric replication of mtDNA, …

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Determinants Of Local Calcium Signaling In Retinal Amacrine Cells, Madhumita Sen Jan 2006

Determinants Of Local Calcium Signaling In Retinal Amacrine Cells, Madhumita Sen

LSU Doctoral Dissertations

Amacrine cells mediate complex lateral signaling in the inner plexiform layer of the vertebrate retina. Glutamate is the primary excitatory neurotransmitter that shapes the retinal circuitry by activating ionotropic glutamate receptors (iGluRs)and metabotropic glutamate receptors (mGluRs). One step in understanding the signaling in the inner retina is to understand the role and expression patterns of these glutamate receptors. The expression of iGluRs in amacrine cells is well documented in the literature. This study addresses the localization of Group I mGluRs in the retina. Pre-embedding immunocytochemistry combined with electron microscopy was used to study the expression of Group I mGluRs in …

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Self-Assembly And Its Inhibition In Bolaform Amphiphilic Dendrimers, Jirun Sun Jan 2006

Self-Assembly And Its Inhibition In Bolaform Amphiphilic Dendrimers, Jirun Sun

LSU Doctoral Dissertations

Self-assembly is scientifically interesting, technologically important, and sometimes life-threatening. A striking example: Alzheimer’s disease is caused by the self-assembly of the â-amyloid fragment of amyloid precursor protein. Such natural amphiphiles normally are very expensive and complicated to isolate. Synthetic mimics that are much cheaper and easier to prepare can facilitate the development of efficient characterization methods, even as they suggest new materials science applications. Two-directional arborols are synthetic bolaform amphiphilic dendrimers. They dissolve in hot water and form reversible gels at room temperature. Bundles of fibrils, which are made by self-assembly of arborols, are found in the gels. The properties …

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A Computational Framework For The Structural Change Analysis Of 3d Volumes Of Microscopic Specimens, Madhusudhanan Balasubramanian Jan 2006

A Computational Framework For The Structural Change Analysis Of 3d Volumes Of Microscopic Specimens, Madhusudhanan Balasubramanian

LSU Doctoral Dissertations

Glaucoma, commonly observed with an elevation in the intraocular pressure level (IOP), is one of the leading causes of blindness. The lamina cribrosa is a mesh-like structure that provides axonal support for the optic nerves leaving the eye. The changes in the laminar structure under IOP elevations may result in the deaths of retinal ganglion cells, leading to vision degradation and loss. We have developed a comprehensive computational framework that can assist the study of structural changes in microscopic structures such as lamina cribrosa. The optical sectioning property of a confocal microscope facilitates imaging thick microscopic specimen at various depths …

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Development Of Spanish L2 Competence In A Synchronous Cmc (Chat Room) Environment: The Role Of Visually-Enhanced Recasts In Fostering Grammatical Knowledge And Changes In Communicative Language Use, Francisco Ramon Lluna-Mateu Jan 2006

Development Of Spanish L2 Competence In A Synchronous Cmc (Chat Room) Environment: The Role Of Visually-Enhanced Recasts In Fostering Grammatical Knowledge And Changes In Communicative Language Use, Francisco Ramon Lluna-Mateu

LSU Doctoral Dissertations

Taking into consideration some gaps observed in SLA research –noticing, recasts, input enhancement (IE),…– and in CALL/CMC research, a study was conducted among 12 advanced FL Spanish learners to assess whether and how, by communicating with a Spanish native speaker in 5 chat-room sessions, their language competence would develop in the following areas: 1) communication strategies; 2) communicative acts; and 3) grammatical knowledge of verb tense-aspect-mood (TAM) assignation. Subjects were assigned to a specific feedback condition/group (A: +recast, -enhancement; B: +recast, +enhancement; and C: no feedback) under which their TAM errors were treated in the sessions. Few research studies have …

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Reliability Of Determining Adults From Juvenile Ducks By Presence Or Absence Of Notched Tail Feathers In Various Species Of North American Ducks, Thomas Michael Siwarski Jan 2006

Reliability Of Determining Adults From Juvenile Ducks By Presence Or Absence Of Notched Tail Feathers In Various Species Of North American Ducks, Thomas Michael Siwarski

LSU Master's Theses

I estimated when juvenile tail molt occurs for several commonly harvested duck species, mallards (Anas platyrhynchos), northern pintails (A. acuta) (hereafter referred to as pintails), gadwalls (A. strepera), lesser scaup (Aythya affinis), and redheads (A. americana). Tests showed that aging mallards by notched tail feathers became unreliable the earliest (early September), followed closely by pintails (mid-late September), with gadwalls being the latest of the dabbling ducks to lose reliability in aging by notched tail feathers (late November). Lesser scaup and redheads retained their notched tail feathers throughout January and the completion of hunting season. Based on initial banding and band …

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Studies Of Epigenetic Instability In Human Normal And Diseased Vulva Skin, Zhengyu Zhang Jan 2006

Studies Of Epigenetic Instability In Human Normal And Diseased Vulva Skin, Zhengyu Zhang

LSU Master's Theses

Epigenetic modification is another mechanism involved in the cancer development besides classic mutations such as deletion. Aberrant promoter methylation and associated chromatin modification have been frequently reported in various tumors of different clinical stages. Hypermethylation has been frequently observed in tumor suppressor genes and causes reduced transcripts of these genes. Hypomethylation also has been reported to be involved in activation of oncogenes. Inactivation of the X chromosome and the imprinting of gamete DNA depend on the methylation patterns in the promoter region. Understanding methylation mechanisms could be helpful to diagnosis of early stage tumorgenesis or offer molecular markers for detecting …

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Lock'em Up And Throw Away The Key: Racial Attitudes And The Structural Determinants Of Support For Crime Policy Among White Americans, Amanda J. Abraham Jan 2006

Lock'em Up And Throw Away The Key: Racial Attitudes And The Structural Determinants Of Support For Crime Policy Among White Americans, Amanda J. Abraham

LSU Doctoral Dissertations

This study investigates support for the death penalty and federal crime spending among white Americans. Data from the American National Election Studies (ANES) series (1992-2000) are matched with census tract level indicators of demographic and community characteristics from the 1990 and 2000 Census Bureau Summary File Tape 3A and county level crime data supplied by the Federal Bureau of Investigation�s Uniform Crime Reports (UCR). Ordered logistic regression is used to investigate five general research questions: (1) Are racial attitudes the most salient individual level predictors of support for crime policy among whites as suggested by prior research? (2) Are whites� …

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Resilient Optical Multicasting Utilizing Cycles In Wdm Optical Networks, Abhishek Satyedranath Jan 2006

Resilient Optical Multicasting Utilizing Cycles In Wdm Optical Networks, Abhishek Satyedranath

LSU Master's Theses

High capacity telecommunications of today is possible only because of the presence of optical networks. At the heart of an optical network is an optical fiber whose data carrying capabilities are unparalleled. Multicasting is a form of communication in wavelength division multiplexed (WDM) networks that involves one source and multiple destinations. Light trees, which employ light splitting at various nodes, are used to deliver data to multiple destinations. A fiber cut has been estimated to occur, on an average, once every four days by TEN, a pan-European carrier network. This thesis presents algorithms to make multicast sessions survivable against component …

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River, Tidal And Wind Interactions In A Deltaic Estuarine System, Gregg Snedden Jan 2006

River, Tidal And Wind Interactions In A Deltaic Estuarine System, Gregg Snedden

LSU Doctoral Dissertations

The balance between river and marine influences is important in governing landscape sustainability in river deltas. River- and atmospherically driven sea level variability, sediment loading, and estuary-ocean exchange in the Mississippi River delta are examined in this study. Subtidal estuarine sea level variability in the Breton Sound estuary was driven by a combination of remote atmospheric forcing outside the estuary over the continental shelf and controlled river inputs through a gated diversion structure at the estuary head. The highly-frictional deltaic landscape acted as a low-pass filter to coastal fluctuations near the estuary mouth. When substantial quantities river water were discharged …

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Retention And Transport Of Arsenic In Soils, Hua Zhang Jan 2006

Retention And Transport Of Arsenic In Soils, Hua Zhang

LSU Doctoral Dissertations

Arsenic transport in soils and aquifers is highly dependent on the adsorption-desorption reactions in the solid phase. Results from our kinetic batch experiments indicated that adsorption of arsenate [As(V)] was highly nonlinear and strongly kinetic. Desorption of As(V) were hysteretic in nature and a significant amount of As(V) was irreversibly adsorbed on all soils. Results from column experiments indicated strong As(V) retardation followed by slow release or extensive tailing of the breakthrough curves (BTCs). Sharp decrease in As(V) concentration during flow interruption verified the extensive non-equilibrium condition which was likely due to the dominance of kinetic retention processes. We evaluated …

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Cultivar, Juice Extraction, Ultra Violet Irradiation And Storage Influence The Stilbene Content Of Muscadine Grapes (Vitis Rotundifolia Michx.), Mark Rene' Leblanc Jan 2006

Cultivar, Juice Extraction, Ultra Violet Irradiation And Storage Influence The Stilbene Content Of Muscadine Grapes (Vitis Rotundifolia Michx.), Mark Rene' Leblanc

LSU Doctoral Dissertations

Stilbene concentration was analyzed in juice and tissue of muscadine grape (Vitis rotundifolia Michx.) and bunch grape (Vitis labrusca L.) from fresh fruit, after processing and after postharvest treatments. Five filter types were evaluated for percent resveratrol recovery when filtering standard and spiked juice samples in preparation for HPLC analysis. Only two (polycarbonate and Anopore) of the five filter types had more than 90 percent recovery. Polycarbonate was chosen for sample preparation since it was more durable during handling. Eight muscadine grape cultivars and three bunch grape cultivars were evaluated. Skin tissue had approximately 100 times higher stilbene concentration than …

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Characterization Of Beta-Amyloid Peptide Aggregation And Acceleration With Non-Fibrillar Forming Peptide-Based Mediators, Nadia J. Edwin Jan 2006

Characterization Of Beta-Amyloid Peptide Aggregation And Acceleration With Non-Fibrillar Forming Peptide-Based Mediators, Nadia J. Edwin

LSU Doctoral Dissertations

Protein aggregation occurs under many circumstances, from the dynamic assembly of tubulin to form microtubules, the aggregation of actin into filaments, as well as plaque formation by amyloid precipitation. One important requirement in studying the mechanism of amyloid aggregation is the ability to monitor the growth kinetics over a wide range in size scales (10 nm to microns) with time that spans microseconds to seconds. Understanding the mechanisms of the aggregation may then lead to improved design of drugs to help control or suppress the aggregation process. In this dissertation, the physical characterization of the â-amyloid peptide and its interaction …

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Accelerating Micro-Scale Pcr (Polymerase Chain Reactor) For Modular Lab-On-A-Chip System, Pin-Chuan Chen Jan 2006

Accelerating Micro-Scale Pcr (Polymerase Chain Reactor) For Modular Lab-On-A-Chip System, Pin-Chuan Chen

LSU Master's Theses

The polymerase chain reaction (PCR) is a powerful technique used to exponentially amplify specific DNA sequences of interest through repetitive temperature cycling. The temperatures used in PCR are typically 90°C - 94°C for denaturation, 50°C - 70°C for renaturation, and 70°C - 75°C for extension. The double-stranded helix of nucleotides, carrying the genetic information, is separated during denaturation, reacts with chemical primers during renaturation, and becomes a complete, replica, double-stranded DNA helix structure during extension. The primary drawback of current commercial benchtop PCR machine is its cycle time due to its large thermal capacitance, and micro PCR is under developing …

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